Rh7DG474400
NAC Family

Inactive rhomboid protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0014538
Physical Location & Seq
Forward (+)
67842418 .. 67845822
3405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG474400.1

Sequence Viewer

Length: 1074 bp
ATGGCCGCCGAGCCACCACCGTCGAAGCTCCACACCCAGATCGACATCACCGCTCCTCCGCCGTGCTCCGCCGACGACTCCTCCTTGGCCACCGAGACCCTCCGCGAACAGAGGCTCTCCTTCTTCGCCTCCCGCTACACTCGCAGCCGCCGCCGCCGGAGCGACACGTGGATCATCTCGCTCTTCGTCATCCTCCACGTCCTGGCCTTCGCTTTGACCATGGCGGTCAACGACTGCTGGCGCAAGTCTCACGGCGACTGCGCTCTCGGAGCCTTTGGGAGACTGTCGTTTCAGCCGCTCCGGGAAAACCCTCTCCTCGGTCCTTCGGCTTCCACGCTTGATGAAGTAGGAGCTCTTCGACGGACTTTTTTGACAGAGAATCATAAAACTTGGCGCCTCTTCACATTTCCATGTCTGCATGCTGGAGCCATCCACCTCGTGATCAACCTTTGCAGTGTTGTCTTCATTGGAATTCACTTGGAGCAAGAGTTCGGAACATTAATTGCAGGGTTAATCTATGTACTGTCTTCTTTCAATGGTACATTGGTGGCTGCACTTTTTGTTGAGAAACGCCCAGCAGTGGGTTCTTCCGGGGCTCTATATGGGTTACTTGGAGCTACACTTTCTGCTCTGATTCAGAACTGGGACTTATACACCAACAAGTTCACAGCTCTCACATCAGTATTACTTGTCGCCATTGGAAATTTCCTCCTTGGTTTGCTACCTTATGTGGATAACTTTTCAAACCTTGGGGGTTTTCTATCAGGGTTCCTACTCGGATCTGCGCTTTTATCCAGTCCTCAAGTCAGAGAAGTGGTGCAAGGAAAAGGAGGCCTCCTGGAGAATGATCTTAAAAGCTCTATCAAGTCGAGGTTCAGGCAGAAGCTGGACAGACCAGTCTATAGAAGTGTCTGTCTTTTTCTCTTTGTACTTATGCTTGCAGGATGTCTTGTAGCAGTTTTGCAGGGCATTGACATGAACCAGTACTGCAAATGGTGTCGTTATGTTGACTGCGTGCCTTCCAAAAGATGGAGCTGCAGTGAAATGGAAGCTTTTTGTGAGGTATGCAACTGA

Protein Analysis

357

Amino Acids

39.43

Weight (kDa)

8.67

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 128 - 264 2.2e-31 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014538)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77860 AT1G77860
fragaria_vesca FvH4_5g38430
malus_domestica MD08G1173800.v1.1
prunus_persica Prupe.1G507800_v2.0.a1 Prupe.1G507800_v2.0.a1 Prupe.1G507800_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0240461
rosa_laevigata RLG00000000677
rosa_multiflora Rmu_sc0005327.1_g000015
rosa_roxburghii Rroxscaffold_3G00219970
rosa_rugosa Rorug07G0336300
rosa_samantha Rh7AG490600 Rh7BG463300 Rh7CG506800 Rh7DG474400
rosa_wichuraiana Rw7G041120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 896
AccB1I GGYRCC 1 cut(s) 393
AccB7I CCANNNNNTGG 2 cut(s) 202, 1029
AccBSI CCGCTC 2 cut(s) 53, 298
AccII CGCG 1 cut(s) 105
AclWI GGATC 2 cut(s) 179, 787
AcoI YGGCCR 2 cut(s) 3, 87
AcsI RAATTY 2 cut(s) 471, 703
AcvI CACGTG 1 cut(s) 168
AcyI GRCGYC 1 cut(s) 394
AdeI CACNNNGTG 1 cut(s) 439
AfaI GTAC 4 cut(s) 522, 541, 930, 986
AfiI CCNNNNNNNGG 5 cut(s) 202, 317, 580, 581, 1029
AflIII ACRYGT 1 cut(s) 165
AgsI TTSAA 2 cut(s) 535, 744
AjiI CACGTC 1 cut(s) 199
AjnI CCWGG 2 cut(s) 201, 837
AloI GAACNNNNNNTCC 2 cut(s) 473, 505
AluBI AGCT 8 cut(s) 28, 353, 617, 671, 858, 886, 1035, 1052
AluI AGCT 8 cut(s) 28, 353, 617, 671, 858, 886, 1035, 1052
Alw21I GWGCWC 2 cut(s) 68, 355
Alw26I GTCTC 3 cut(s) 89, 252, 274
AlwI GGATC 2 cut(s) 179, 787
AlwNI CAGNNNCTG 1 cut(s) 886
AoxI GGCC 4 cut(s) 3, 87, 204, 832
ApeKI GCWGC 3 cut(s) 144, 551, 1035
ApoI RAATTY 2 cut(s) 471, 703
AseI ATTAAT 1 cut(s) 500
AspLEI GCGC 4 cut(s) 243, 263, 396, 787
AspS9I GGNCC 1 cut(s) 320
AsuC2I CCSGG 2 cut(s) 302, 592
AsuHPI GGTGA 1 cut(s) 40
AvaII GGWCC 1 cut(s) 320
BaeI ACNNNNGTAYC 2 cut(s) 531, 564
BalI TGGCCA 1 cut(s) 89
BanI GGYRCC 1 cut(s) 393
BanII GRGCYC 2 cut(s) 355, 598
BarI GAAGNNNNNNTAC 2 cut(s) 339, 371
BauI CACGAG 1 cut(s) 437
BbrPI CACGTG 1 cut(s) 168
BbsI GAAGAC 2 cut(s) 454, 519
Bbv12I GWGCWC 2 cut(s) 68, 355
BbvI GCAGC 3 cut(s) 156, 538, 1022
BccI CCATC 2 cut(s) 437, 1023
BceAI ACGGC 2 cut(s) 46, 268
BciT130I CCWGG 2 cut(s) 203, 839
BclI TGATCA 1 cut(s) 441
BcnI CCSGG 2 cut(s) 302, 592
BcoDI GTCTC 3 cut(s) 89, 252, 274
BfmI CTRYAG 2 cut(s) 901, 1036
BfoI RGCGCY 1 cut(s) 397
BisI GCNGC 8 cut(s) 6, 145, 148, 151, 154, 296, 552, 1036
BlsI GCNGC 8 cut(s) 7, 146, 149, 152, 155, 297, 553, 1037
BmcAI AGTACT 1 cut(s) 986
Bme1390I CCNGG 4 cut(s) 203, 302, 592, 839
Bme18I GGWCC 1 cut(s) 320
BmgBI CACGTC 1 cut(s) 199
BmgT120I GGNCC 1 cut(s) 320
BmiI GGNNCC 4 cut(s) 271, 395, 427, 770
BmrFI CCNGG 4 cut(s) 203, 302, 592, 839
BmrI ACTGGG 1 cut(s) 652
BmuI ACTGGG 1 cut(s) 652
BpiI GAAGAC 2 cut(s) 454, 519
BpmI CTGGAG 2 cut(s) 444, 860
BpuEI CTTGAG 1 cut(s) 786
BpuMI CCSGG 2 cut(s) 302, 592
BsaAI YACGTR 1 cut(s) 168
BsaBI GATNNNNATC 1 cut(s) 44
BsaHI GRCGYC 1 cut(s) 394
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 6 cut(s) 84, 219, 316, 591, 712, 748
BsaXI ACNNNNNCTCC 8 cut(s) 40, 65, 70, 95, 473, 503, 1024, 1054
Bsc4I CCNNNNNNNGG 5 cut(s) 202, 317, 580, 581, 1029
Bse1I ACTGG 4 cut(s) 647, 795, 896, 982
Bse8I GATNNNNATC 1 cut(s) 44
BseBI CCWGG 2 cut(s) 203, 839
BseDI CCNNGG 6 cut(s) 84, 219, 316, 591, 712, 748
BseGI GGATG 3 cut(s) 189, 429, 950
BseJI GATNNNNATC 1 cut(s) 44
BseLI CCNNNNNNNGG 5 cut(s) 202, 317, 580, 581, 1029
BseNI ACTGG 4 cut(s) 647, 795, 896, 982
BseRI GAGGAG 3 cut(s) 45, 70, 305
BseXI GCAGC 3 cut(s) 156, 538, 1022
BseYI CCCAGC 1 cut(s) 574
BsgI GTGCAG 1 cut(s) 537
Bsh1236I CGCG 1 cut(s) 105
BshFI GGCC 4 cut(s) 5, 89, 206, 834
BshNI GGYRCC 1 cut(s) 393
BsiHKAI GWGCWC 2 cut(s) 68, 355
BsiSI CCGG 3 cut(s) 157, 301, 591
BslFI GGGAC 1 cut(s) 659
BslI CCNNNNNNNGG 5 cut(s) 202, 317, 580, 581, 1029
BsmAI GTCTC 3 cut(s) 89, 252, 274
BsmFI GGGAC 1 cut(s) 659
BsnI GGCC 4 cut(s) 5, 89, 206, 834
Bso31I GGTCTC 1 cut(s) 89
Bsp1286I GDGCHC 3 cut(s) 68, 355, 598
Bsp143I GATC 5 cut(s) 39, 171, 441, 779, 847
Bsp19I CCATGG 1 cut(s) 219
BspANI GGCC 4 cut(s) 5, 89, 206, 834
BspFNI CGCG 1 cut(s) 105
BspLI GGNNCC 4 cut(s) 271, 395, 427, 770
BspMAI CTGCAG 1 cut(s) 1040
BspPI GGATC 2 cut(s) 179, 787
BspQI GCTCTTC 2 cut(s) 188, 360
BspT107I GGYRCC 1 cut(s) 393
BspTNI GGTCTC 1 cut(s) 89
BsrBI CCGCTC 2 cut(s) 53, 298
BsrI ACTGG 4 cut(s) 647, 795, 896, 982
BssECI CCNNGG 6 cut(s) 84, 219, 316, 591, 712, 748
BssMI GATC 5 cut(s) 39, 171, 441, 779, 847
BssNI GRCGYC 1 cut(s) 394
BssSI CACGAG 1 cut(s) 437
BssT1I CCWWGG 4 cut(s) 84, 219, 712, 748
Bst2BI CACGAG 1 cut(s) 437
Bst2UI CCWGG 2 cut(s) 203, 839
Bst4CI ACNGT 3 cut(s) 21, 285, 525
Bst6I CTCTTC 3 cut(s) 188, 360, 404
BstACI GRCGYC 1 cut(s) 394
BstBAI YACGTR 1 cut(s) 168
BstC8I GCNNGC 4 cut(s) 239, 420, 939, 1016
BstDSI CCRYGG 1 cut(s) 219
BstF5I GGATG 3 cut(s) 189, 429, 950
BstFNI CGCG 1 cut(s) 105
BstH2I RGCGCY 1 cut(s) 397
BstHHI GCGC 4 cut(s) 243, 263, 396, 787
BstKTI GATC 5 cut(s) 42, 174, 444, 782, 850
BstMAI GTCTC 3 cut(s) 89, 252, 274
BstMBI GATC 5 cut(s) 39, 171, 441, 779, 847
BstMWI GCNNNNNNNGC 5 cut(s) 141, 150, 153, 159, 269
BstNI CCWGG 2 cut(s) 203, 839
BstNSI RCATGY 1 cut(s) 422
BstSCI CCNGG 4 cut(s) 201, 300, 590, 837
BstSFI CTRYAG 2 cut(s) 901, 1036
BstUI CGCG 1 cut(s) 105
BstV1I GCAGC 3 cut(s) 156, 538, 1022
BstV2I GAAGAC 2 cut(s) 454, 519
BstX2I RGATCY 1 cut(s) 779
BstYI RGATCY 1 cut(s) 779
BsuRI GGCC 4 cut(s) 5, 89, 206, 834
BtgI CCRYGG 1 cut(s) 219
BtrI CACGTC 1 cut(s) 199
BtsCI GGATG 3 cut(s) 189, 429, 950
BtsI GCAGTG 3 cut(s) 460, 585, 1045
BtsIMutI CAGTG 3 cut(s) 460, 585, 1045
Cac8I GCNNGC 4 cut(s) 239, 420, 939, 1016
CaiI CAGNNNCTG 1 cut(s) 886
CfoI GCGC 4 cut(s) 243, 263, 396, 787
Cfr13I GGNCC 1 cut(s) 320
Csp6I GTAC 4 cut(s) 521, 540, 929, 985
CviAII CATG 4 cut(s) 220, 411, 419, 976
CviQI GTAC 4 cut(s) 521, 540, 929, 985
DinI GGCGCC 1 cut(s) 395
DpnI GATC 5 cut(s) 41, 173, 443, 781, 849
DpnII GATC 5 cut(s) 39, 171, 441, 779, 847
DraIII CACNNNGTG 1 cut(s) 439
DrdI GACNNNNNNGTC 1 cut(s) 896
DseDI GACNNNNNNGTC 1 cut(s) 896
EaeI YGGCCR 2 cut(s) 3, 87
Eam1104I CTCTTC 3 cut(s) 188, 360, 404
EarI CTCTTC 3 cut(s) 188, 360, 404
EciI GGCGGA 2 cut(s) 48, 58
Ecl136II GAGCTC 1 cut(s) 353
Eco130I CCWWGG 4 cut(s) 84, 219, 712, 748
Eco147I AGGCCT 1 cut(s) 834
Eco24I GRGCYC 2 cut(s) 355, 598
Eco31I GGTCTC 1 cut(s) 89
Eco47I GGWCC 1 cut(s) 320
Eco53kI GAGCTC 1 cut(s) 353
Eco72I CACGTG 1 cut(s) 168
EcoICRI GAGCTC 1 cut(s) 353
EcoRI GAATTC 1 cut(s) 471
EcoRII CCWGG 2 cut(s) 201, 837
EcoT14I CCWWGG 4 cut(s) 84, 219, 712, 748
EcoT38I GRGCYC 2 cut(s) 355, 598
EgeI GGCGCC 1 cut(s) 395
EheI GGCGCC 1 cut(s) 395
ErhI CCWWGG 4 cut(s) 84, 219, 712, 748
FaeI CATG 4 cut(s) 223, 414, 422, 979
FaqI GGGAC 1 cut(s) 659
FatI CATG 4 cut(s) 219, 410, 418, 975
FauI CCCGC 1 cut(s) 140
FbaI TGATCA 1 cut(s) 441
Fnu4HI GCNGC 8 cut(s) 6, 145, 148, 151, 154, 296, 552, 1036
FokI GGATG 3 cut(s) 176, 416, 957
FriOI GRGCYC 2 cut(s) 355, 598
Fsp4HI GCNGC 8 cut(s) 6, 145, 148, 151, 154, 296, 552, 1036
GlaI GCGC 4 cut(s) 242, 262, 395, 786
GluI GCNGC 8 cut(s) 6, 145, 148, 151, 154, 296, 552, 1036
GsaI CCCAGC 1 cut(s) 578
GsuI CTGGAG 2 cut(s) 444, 860
HaeII RGCGCY 1 cut(s) 397
HaeIII GGCC 4 cut(s) 5, 89, 206, 834
HapII CCGG 3 cut(s) 157, 301, 591
HhaI GCGC 4 cut(s) 243, 263, 396, 787
Hin1I GRCGYC 1 cut(s) 394
Hin1II CATG 4 cut(s) 223, 414, 422, 979
Hin6I GCGC 4 cut(s) 241, 261, 394, 785
HinP1I GCGC 4 cut(s) 241, 261, 394, 785
HincII GTYRAC 2 cut(s) 229, 1009
HindII GTYRAC 2 cut(s) 229, 1009
HindIII AAGCTT 1 cut(s) 1050
HinfI GANTC 3 cut(s) 77, 379, 634
HpaII CCGG 3 cut(s) 157, 301, 591
HphI GGTGA 1 cut(s) 40
Hpy166II GTNNAC 3 cut(s) 229, 666, 1009
Hpy188I TCNGA 6 cut(s) 269, 494, 633, 639, 779, 809
Hpy188III TCNNGA 1 cut(s) 439
Hpy8I GTNNAC 3 cut(s) 229, 666, 1009
Hpy99I CGWCG 3 cut(s) 25, 77, 363
HpyAV CCTTC 4 cut(s) 130, 217, 333, 1029
HpyCH4III ACNGT 3 cut(s) 21, 285, 525
HpyCH4IV ACGT 2 cut(s) 167, 198
HpyF10VI GCNNNNNNNGC 5 cut(s) 141, 150, 153, 159, 269
HpySE526I ACGT 2 cut(s) 167, 198
Hsp92I GRCGYC 1 cut(s) 394
Hsp92II CATG 4 cut(s) 223, 414, 422, 979
HspAI GCGC 4 cut(s) 241, 261, 394, 785
KasI GGCGCC 1 cut(s) 393
Ksp22I TGATCA 1 cut(s) 441
Kzo9I GATC 5 cut(s) 39, 171, 441, 779, 847
LguI GCTCTTC 2 cut(s) 188, 360
Lsp1109I GCAGC 3 cut(s) 156, 538, 1022
MaeII ACGT 2 cut(s) 167, 198
MaeIII GTNAC 1 cut(s) 606
MalI GATC 5 cut(s) 41, 173, 443, 781, 849
MbiI CCGCTC 2 cut(s) 53, 298
MboI GATC 5 cut(s) 39, 171, 441, 779, 847
MboII GAAGA 7 cut(s) 115, 175, 347, 391, 454, 519, 579
MflI RGATCY 1 cut(s) 779
MhlI GDGCHC 3 cut(s) 68, 355, 598
MlsI TGGCCA 1 cut(s) 89
MluCI AATT 3 cut(s) 471, 501, 703
MluNI TGGCCA 1 cut(s) 89
Mly113I GGCGCC 1 cut(s) 394
MlyI GAGTC 1 cut(s) 71
Mox20I TGGCCA 1 cut(s) 89
MscI TGGCCA 1 cut(s) 89
MseI TTAA 3 cut(s) 500, 512, 852
MslI CAYNNNNRTG 2 cut(s) 409, 974
Msp20I TGGCCA 1 cut(s) 89
MspI CCGG 3 cut(s) 157, 301, 591
MspR9I CCNGG 4 cut(s) 203, 302, 592, 839
MvaI CCWGG 2 cut(s) 203, 839
MvnI CGCG 1 cut(s) 105
MwoI GCNNNNNNNGC 5 cut(s) 141, 150, 153, 159, 269
NarI GGCGCC 1 cut(s) 394
NciI CCSGG 2 cut(s) 302, 592
NcoI CCATGG 1 cut(s) 219
NdeII GATC 5 cut(s) 39, 171, 441, 779, 847
NlaIII CATG 4 cut(s) 223, 414, 422, 979
NlaIV GGNNCC 4 cut(s) 271, 395, 427, 770
NmeAIII GCCGAG 1 cut(s) 34
NspI RCATGY 1 cut(s) 422
PaeI GCATGC 1 cut(s) 422
PceI AGGCCT 1 cut(s) 834
PciSI GCTCTTC 2 cut(s) 188, 360
PfeI GAWTC 2 cut(s) 379, 634
PflMI CCANNNNNTGG 2 cut(s) 202, 1029
PfoI TCCNGGA 2 cut(s) 300, 837
PkrI GCNGC 8 cut(s) 7, 146, 149, 152, 155, 297, 553, 1037
PleI GAGTC 1 cut(s) 71
PluTI GGCGCC 1 cut(s) 397
PmaCI CACGTG 1 cut(s) 168
PmlI CACGTG 1 cut(s) 168
PpsI GAGTC 1 cut(s) 71
Ppu21I YACGTR 1 cut(s) 168
PshBI ATTAAT 1 cut(s) 500
Psp124BI GAGCTC 1 cut(s) 355
Psp6I CCWGG 2 cut(s) 201, 837
PspCI CACGTG 1 cut(s) 168
PspFI CCCAGC 1 cut(s) 574
PspGI CCWGG 2 cut(s) 201, 837
PspN4I GGNNCC 4 cut(s) 271, 395, 427, 770
PspPI GGNCC 1 cut(s) 320
PstI CTGCAG 1 cut(s) 1040
PstNI CAGNNNCTG 1 cut(s) 886
PsuI RGATCY 1 cut(s) 779
RsaI GTAC 4 cut(s) 522, 541, 930, 986
RsaNI GTAC 4 cut(s) 521, 540, 929, 985
RseI CAYNNNNRTG 2 cut(s) 409, 974
SacI GAGCTC 1 cut(s) 355
SapI GCTCTTC 2 cut(s) 188, 360
SaqAI TTAA 3 cut(s) 500, 512, 852
SatI GCNGC 8 cut(s) 6, 145, 148, 151, 154, 296, 552, 1036
Sau3AI GATC 5 cut(s) 39, 171, 441, 779, 847
Sau96I GGNCC 1 cut(s) 320
ScaI AGTACT 1 cut(s) 986
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 4 cut(s) 203, 302, 592, 839
SduI GDGCHC 3 cut(s) 68, 355, 598
SfcI CTRYAG 2 cut(s) 901, 1036
SfoI GGCGCC 1 cut(s) 395
SinI GGWCC 1 cut(s) 320
SmiMI CAYNNNNRTG 2 cut(s) 409, 974
SmlI CTYRAG 1 cut(s) 801
SmoI CTYRAG 1 cut(s) 801
SphI GCATGC 1 cut(s) 422
Sse9I AATT 3 cut(s) 471, 501, 703
SseBI AGGCCT 1 cut(s) 834
SspDI GGCGCC 1 cut(s) 393
SstI GAGCTC 1 cut(s) 355
StuI AGGCCT 1 cut(s) 834
StyD4I CCNGG 4 cut(s) 201, 300, 590, 837
StyI CCWWGG 4 cut(s) 84, 219, 712, 748
TaaI ACNGT 3 cut(s) 21, 285, 525
TaiI ACGT 2 cut(s) 170, 201
TaqI TCGA 4 cut(s) 23, 42, 358, 869
TaqII GACCGA 1 cut(s) 308
TasI AATT 3 cut(s) 471, 501, 703
TatI WGTACW 3 cut(s) 520, 928, 984
TauI GCSGC 5 cut(s) 8, 150, 153, 156, 298
TfiI GAWTC 2 cut(s) 379, 634
Tru1I TTAA 3 cut(s) 500, 512, 852
Tru9I TTAA 3 cut(s) 500, 512, 852
TscAI CASTG 3 cut(s) 460, 585, 1045
TseI GCWGC 3 cut(s) 144, 551, 1035
TspDTI ATGAA 3 cut(s) 357, 454, 992
TspGWI ACGGA 1 cut(s) 376
TspRI CASTG 3 cut(s) 460, 585, 1045
Van91I CCANNNNNTGG 2 cut(s) 202, 1029
VpaK11BI GGWCC 1 cut(s) 320
VspI ATTAAT 1 cut(s) 500
XapI RAATTY 2 cut(s) 471, 703
XceI RCATGY 1 cut(s) 422
ZrmI AGTACT 1 cut(s) 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.