Rroxscaffold_3G00219970
NAC Family

Inactive rhomboid protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014538
Physical Location & Seq
Reverse (-)
2143003 .. 2146398
3396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00219970.1

Sequence Viewer

Length: 1077 bp
ATGGCCGCCGAGCCACCGCCGTTGAAGCTCCACACCCAGGTCGACATCAACGCTCCTCCGCCGTGCTCCGCCGACGACTCCTCCTCCTTGGCCACCGAAACCCTCCGCGAACAGAGGCTCTCCTTCTTCGCCTCCCGCTACACTCGCCGCCGCCGCCGCCGGCGGAGCGACACGTGGATCATCTCTCTCTTCGTCATCCTCCACGTCCTGGCCTTCGCGTTGACCATGGCCGTCAACGACTGCTGGCGCAAGTCTCACGGCGACTGCGCTCTCGGAGCCTTTGGGAGACTGTCGTTTCAGCCGCTCCGGGAAAACCCTCTCCTCGGCCCTTCGGCTTCCACATTTAGGGTTTACTTGATGAATCTTTCAGTAATTTCTTTGATGTCTGGAGTGATTGCAGGGTTAATCTATGTACTGTCTTCTTTCAATGGTACATTGGTGGCTGCACTTTTTGTTGAGAAACGCCCAGCAGTGGGTTCTTCCGGGGCTCTATATGGGTTACTTGGAGCTACACTTTCTGCTCTGATTCAGAACTGGGACTTATACACCAACAAGTTCACAGCTCTCACATCAGTTTTACTTGTCACCATTGGAAATTTCCTCCTTGGTTTGCTACCTTATGTGGATAACTTTTCAAACCTTGGGGGTTTTCTATCAGGGTTCCTACTCGGATCTGCACTTTTATCCAGTCCTCAAGTCAGAGAAGTGGTGCAAGGAAAAGGAGGCCTCCTGGACAATGATCTTAAAAGCTCTATCAAGTCGAGGTTCAGGCAGAAGCTGGACAGACCAGTCTATAGAAGTGTCTGTCTTTTTCTCTTTGTACTTATGCTTGCAGGATGTCTTGTAGCAGTTTTGCAGGGCATTGACATGAACCAGTACTGCAAATGGTGTCGTTATGTTGACTGCATGCCTTCCAAAAGATGGAGCTGCAGCGAAATGGAAGCTTTTTGTGAGACCATGGTGAGCCATGAACAGATGACTTTGACCTGTATGGGTAATGGGAACTTCAAGGTTCTTCCTTTCACCAACATTTCTCAAGCCAGGATGAATGACTTGTGCAGCATGATATGCTCATAG

Protein Analysis

358

Amino Acids

39.59

Weight (kDa)

9.06

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 128 - 228 2.4e-16 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014538)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77860 AT1G77860
fragaria_vesca FvH4_5g38430
malus_domestica MD08G1173800.v1.1
prunus_persica Prupe.1G507800_v2.0.a1 Prupe.1G507800_v2.0.a1 Prupe.1G507800_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0240461
rosa_laevigata RLG00000000677
rosa_multiflora Rmu_sc0005327.1_g000015
rosa_roxburghii Rroxscaffold_3G00219970
rosa_rugosa Rorug07G0336300
rosa_samantha Rh7AG490600 Rh7BG463300 Rh7CG506800 Rh7DG474400
rosa_wichuraiana Rw7G041120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 788
AccB7I CCANNNNNTGG 2 cut(s) 208, 921
AccBSI CCGCTC 1 cut(s) 304
AccI GTMKAC 1 cut(s) 42
AccII CGCG 2 cut(s) 108, 218
AclWI GGATC 2 cut(s) 185, 679
AcoI YGGCCR 3 cut(s) 3, 90, 228
AcsI RAATTY 1 cut(s) 595
AcvI CACGTG 1 cut(s) 174
AfaI GTAC 4 cut(s) 414, 433, 822, 878
AfiI CCNNNNNNNGG 7 cut(s) 37, 208, 323, 345, 472, 473, 921
AflIII ACRYGT 1 cut(s) 171
AgsI TTSAA 4 cut(s) 25, 427, 636, 1009
AjiI CACGTC 1 cut(s) 205
AjnI CCWGG 4 cut(s) 36, 207, 729, 1040
AluBI AGCT 7 cut(s) 28, 509, 563, 750, 778, 927, 944
AluI AGCT 7 cut(s) 28, 509, 563, 750, 778, 927, 944
Alw21I GWGCWC 1 cut(s) 68
Alw26I GTCTC 3 cut(s) 258, 280, 947
AlwI GGATC 2 cut(s) 185, 679
AlwNI CAGNNNCTG 1 cut(s) 778
AoxI GGCC 6 cut(s) 3, 90, 210, 228, 325, 724
ApeKI GCWGC 4 cut(s) 443, 927, 930, 1059
ApoI RAATTY 1 cut(s) 595
AspLEI GCGC 2 cut(s) 249, 269
AspS9I GGNCC 1 cut(s) 326
AsuC2I CCSGG 2 cut(s) 308, 484
AsuHPI GGTGA 3 cut(s) 577, 973, 1015
BaeI ACNNNNGTAYC 2 cut(s) 423, 456
BalI TGGCCA 1 cut(s) 92
BanII GRGCYC 1 cut(s) 490
BbrPI CACGTG 1 cut(s) 174
BbsI GAAGAC 1 cut(s) 411
Bbv12I GWGCWC 1 cut(s) 68
BbvI GCAGC 4 cut(s) 430, 914, 942, 1071
BccI CCATC 1 cut(s) 915
BceAI ACGGC 4 cut(s) 4, 46, 215, 274
BciT130I CCWGG 4 cut(s) 38, 209, 731, 1042
BcnI CCSGG 2 cut(s) 308, 484
BcoDI GTCTC 3 cut(s) 258, 280, 947
BfmI CTRYAG 2 cut(s) 793, 928
BmcAI AGTACT 1 cut(s) 878
Bme1390I CCNGG 6 cut(s) 38, 209, 308, 484, 731, 1042
BmgBI CACGTC 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 326
BmiI GGNNCC 2 cut(s) 277, 662
BmrFI CCNGG 6 cut(s) 38, 209, 308, 484, 731, 1042
BmrI ACTGGG 1 cut(s) 544
BmuI ACTGGG 1 cut(s) 544
BpiI GAAGAC 1 cut(s) 411
BpmI CTGGAG 1 cut(s) 408
BpuEI CTTGAG 2 cut(s) 678, 1020
BpuMI CCSGG 2 cut(s) 308, 484
BsaAI YACGTR 1 cut(s) 174
BsaI GGTCTC 1 cut(s) 947
BsaJI CCNNGG 8 cut(s) 36, 87, 225, 322, 483, 604, 640, 957
BsaXI ACNNNNNCTCC 4 cut(s) 65, 68, 95, 98
Bsc4I CCNNNNNNNGG 7 cut(s) 37, 208, 323, 345, 472, 473, 921
Bse118I RCCGGY 1 cut(s) 159
Bse1I ACTGG 4 cut(s) 539, 687, 788, 874
BseBI CCWGG 4 cut(s) 38, 209, 731, 1042
BseDI CCNNGG 8 cut(s) 36, 87, 225, 322, 483, 604, 640, 957
BseGI GGATG 3 cut(s) 195, 842, 1050
BseLI CCNNNNNNNGG 7 cut(s) 37, 208, 323, 345, 472, 473, 921
BseNI ACTGG 4 cut(s) 539, 687, 788, 874
BseRI GAGGAG 4 cut(s) 45, 70, 73, 311
BseXI GCAGC 4 cut(s) 430, 914, 942, 1071
BseYI CCCAGC 1 cut(s) 466
BsgI GTGCAG 2 cut(s) 429, 660
Bsh1236I CGCG 2 cut(s) 108, 218
BshFI GGCC 6 cut(s) 5, 92, 212, 230, 327, 726
BsiHKAI GWGCWC 1 cut(s) 68
BsiSI CCGG 3 cut(s) 160, 307, 483
BslFI GGGAC 1 cut(s) 551
BslI CCNNNNNNNGG 7 cut(s) 37, 208, 323, 345, 472, 473, 921
BsmAI GTCTC 3 cut(s) 258, 280, 947
BsmFI GGGAC 1 cut(s) 551
BsnI GGCC 6 cut(s) 5, 92, 212, 230, 327, 726
Bso31I GGTCTC 1 cut(s) 947
Bsp1286I GDGCHC 2 cut(s) 68, 490
Bsp143I GATC 3 cut(s) 177, 671, 739
Bsp19I CCATGG 2 cut(s) 225, 957
BspANI GGCC 6 cut(s) 5, 92, 212, 230, 327, 726
BspFNI CGCG 2 cut(s) 108, 218
BspLI GGNNCC 2 cut(s) 277, 662
BspMAI CTGCAG 1 cut(s) 932
BspPI GGATC 2 cut(s) 185, 679
BspTNI GGTCTC 1 cut(s) 947
BsrBI CCGCTC 1 cut(s) 304
BsrFI RCCGGY 1 cut(s) 159
BsrI ACTGG 4 cut(s) 539, 687, 788, 874
BssAI RCCGGY 1 cut(s) 159
BssECI CCNNGG 8 cut(s) 36, 87, 225, 322, 483, 604, 640, 957
BssMI GATC 3 cut(s) 177, 671, 739
BssT1I CCWWGG 5 cut(s) 87, 225, 604, 640, 957
Bst2UI CCWGG 4 cut(s) 38, 209, 731, 1042
Bst4CI ACNGT 2 cut(s) 291, 417
Bst6I CTCTTC 1 cut(s) 194
BstAPI GCANNNNNTGC 1 cut(s) 1068
BstBAI YACGTR 1 cut(s) 174
BstC8I GCNNGC 4 cut(s) 161, 245, 831, 908
BstDSI CCRYGG 2 cut(s) 225, 957
BstF5I GGATG 3 cut(s) 195, 842, 1050
BstFNI CGCG 2 cut(s) 108, 218
BstHHI GCGC 2 cut(s) 249, 269
BstKTI GATC 3 cut(s) 180, 674, 742
BstMAI GTCTC 3 cut(s) 258, 280, 947
BstMBI GATC 3 cut(s) 177, 671, 739
BstMWI GCNNNNNNNGC 7 cut(s) 25, 144, 153, 156, 165, 275, 1068
BstNI CCWGG 4 cut(s) 38, 209, 731, 1042
BstNSI RCATGY 1 cut(s) 910
BstSCI CCNGG 6 cut(s) 36, 207, 306, 482, 729, 1040
BstSFI CTRYAG 2 cut(s) 793, 928
BstUI CGCG 2 cut(s) 108, 218
BstV1I GCAGC 4 cut(s) 430, 914, 942, 1071
BstV2I GAAGAC 1 cut(s) 411
BstX2I RGATCY 1 cut(s) 671
BstYI RGATCY 1 cut(s) 671
BsuRI GGCC 6 cut(s) 5, 92, 212, 230, 327, 726
BtgI CCRYGG 2 cut(s) 225, 957
BtrI CACGTC 1 cut(s) 205
BtsCI GGATG 3 cut(s) 195, 842, 1050
BtsI GCAGTG 1 cut(s) 477
BtsIMutI CAGTG 1 cut(s) 477
Cac8I GCNNGC 4 cut(s) 161, 245, 831, 908
CaiI CAGNNNCTG 1 cut(s) 778
CfoI GCGC 2 cut(s) 249, 269
Cfr10I RCCGGY 1 cut(s) 159
Cfr13I GGNCC 1 cut(s) 326
Csp6I GTAC 4 cut(s) 413, 432, 821, 877
CspCI CAANNNNNGTGG 1 cut(s) 38
CviAII CATG 6 cut(s) 226, 868, 907, 958, 968, 1063
CviQI GTAC 4 cut(s) 413, 432, 821, 877
DpnI GATC 3 cut(s) 179, 673, 741
DpnII GATC 3 cut(s) 177, 671, 739
DrdI GACNNNNNNGTC 1 cut(s) 788
DseDI GACNNNNNNGTC 1 cut(s) 788
EaeI YGGCCR 3 cut(s) 3, 90, 228
Eam1104I CTCTTC 1 cut(s) 194
EarI CTCTTC 1 cut(s) 194
EciI GGCGGA 3 cut(s) 48, 58, 178
Eco130I CCWWGG 5 cut(s) 87, 225, 604, 640, 957
Eco147I AGGCCT 1 cut(s) 726
Eco24I GRGCYC 1 cut(s) 490
Eco31I GGTCTC 1 cut(s) 947
Eco72I CACGTG 1 cut(s) 174
EcoRII CCWGG 4 cut(s) 36, 207, 729, 1040
EcoT14I CCWWGG 5 cut(s) 87, 225, 604, 640, 957
EcoT38I GRGCYC 1 cut(s) 490
ErhI CCWWGG 5 cut(s) 87, 225, 604, 640, 957
FaeI CATG 6 cut(s) 229, 871, 910, 961, 971, 1066
FaqI GGGAC 1 cut(s) 551
FatI CATG 6 cut(s) 225, 867, 906, 957, 967, 1062
FauI CCCGC 1 cut(s) 143
FblI GTMKAC 1 cut(s) 42
FokI GGATG 3 cut(s) 182, 849, 1057
FriOI GRGCYC 1 cut(s) 490
GlaI GCGC 2 cut(s) 248, 268
GsaI CCCAGC 1 cut(s) 470
GsuI CTGGAG 1 cut(s) 408
HaeIII GGCC 6 cut(s) 5, 92, 212, 230, 327, 726
HapII CCGG 3 cut(s) 160, 307, 483
HhaI GCGC 2 cut(s) 249, 269
Hin1II CATG 6 cut(s) 229, 871, 910, 961, 971, 1066
Hin6I GCGC 2 cut(s) 247, 267
HinP1I GCGC 2 cut(s) 247, 267
HincII GTYRAC 4 cut(s) 43, 222, 235, 901
HindII GTYRAC 4 cut(s) 43, 222, 235, 901
HindIII AAGCTT 1 cut(s) 942
HinfI GANTC 3 cut(s) 77, 361, 526
HpaII CCGG 3 cut(s) 160, 307, 483
HphI GGTGA 3 cut(s) 577, 973, 1015
Hpy166II GTNNAC 6 cut(s) 43, 222, 235, 352, 558, 901
Hpy188I TCNGA 5 cut(s) 275, 525, 531, 671, 701
Hpy188III TCNNGA 1 cut(s) 387
Hpy8I GTNNAC 6 cut(s) 43, 222, 235, 352, 558, 901
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 4 cut(s) 133, 223, 339, 921
HpyCH4III ACNGT 2 cut(s) 291, 417
HpyCH4IV ACGT 2 cut(s) 173, 204
HpyF10VI GCNNNNNNNGC 7 cut(s) 25, 144, 153, 156, 165, 275, 1068
HpySE526I ACGT 2 cut(s) 173, 204
Hsp92II CATG 6 cut(s) 229, 871, 910, 961, 971, 1066
HspAI GCGC 2 cut(s) 247, 267
KroI GCCGGC 1 cut(s) 159
KroNI GCCGGC 1 cut(s) 161
Kzo9I GATC 3 cut(s) 177, 671, 739
LmnI GCTCC 8 cut(s) 33, 58, 71, 165, 275, 309, 506, 924
Lsp1109I GCAGC 4 cut(s) 430, 914, 942, 1071
MaeII ACGT 2 cut(s) 173, 204
MaeIII GTNAC 2 cut(s) 498, 583
MalI GATC 3 cut(s) 179, 673, 741
MbiI CCGCTC 1 cut(s) 304
MboI GATC 3 cut(s) 177, 671, 739
MboII GAAGA 5 cut(s) 118, 181, 411, 471, 1007
MflI RGATCY 1 cut(s) 671
MhlI GDGCHC 2 cut(s) 68, 490
MlsI TGGCCA 1 cut(s) 92
MluCI AATT 2 cut(s) 372, 595
MluNI TGGCCA 1 cut(s) 92
MlyI GAGTC 1 cut(s) 71
Mox20I TGGCCA 1 cut(s) 92
MreI CGCCGGCG 1 cut(s) 159
MroNI GCCGGC 1 cut(s) 159
MscI TGGCCA 1 cut(s) 92
MseI TTAA 2 cut(s) 404, 744
MslI CAYNNNNRTG 1 cut(s) 866
Msp20I TGGCCA 1 cut(s) 92
MspI CCGG 3 cut(s) 160, 307, 483
MspR9I CCNGG 6 cut(s) 38, 209, 308, 484, 731, 1042
MvaI CCWGG 4 cut(s) 38, 209, 731, 1042
MvnI CGCG 2 cut(s) 108, 218
MwoI GCNNNNNNNGC 7 cut(s) 25, 144, 153, 156, 165, 275, 1068
NaeI GCCGGC 1 cut(s) 161
NciI CCSGG 2 cut(s) 308, 484
NcoI CCATGG 2 cut(s) 225, 957
NdeII GATC 3 cut(s) 177, 671, 739
NgoMIV GCCGGC 1 cut(s) 159
NlaIII CATG 6 cut(s) 229, 871, 910, 961, 971, 1066
NlaIV GGNNCC 2 cut(s) 277, 662
NmeAIII GCCGAG 2 cut(s) 34, 303
NmuCI GTSAC 1 cut(s) 583
NspI RCATGY 1 cut(s) 910
PaeI GCATGC 1 cut(s) 910
PceI AGGCCT 1 cut(s) 726
PdiI GCCGGC 1 cut(s) 161
PfeI GAWTC 2 cut(s) 361, 526
PflMI CCANNNNNTGG 2 cut(s) 208, 921
PfoI TCCNGGA 2 cut(s) 306, 729
PleI GAGTC 1 cut(s) 71
PmaCI CACGTG 1 cut(s) 174
PmlI CACGTG 1 cut(s) 174
PpsI GAGTC 1 cut(s) 71
Ppu21I YACGTR 1 cut(s) 174
Psp6I CCWGG 4 cut(s) 36, 207, 729, 1040
PspCI CACGTG 1 cut(s) 174
PspFI CCCAGC 1 cut(s) 466
PspGI CCWGG 4 cut(s) 36, 207, 729, 1040
PspN4I GGNNCC 2 cut(s) 277, 662
PspPI GGNCC 1 cut(s) 326
PstI CTGCAG 1 cut(s) 932
PstNI CAGNNNCTG 1 cut(s) 778
PsuI RGATCY 1 cut(s) 671
RsaI GTAC 4 cut(s) 414, 433, 822, 878
RsaNI GTAC 4 cut(s) 413, 432, 821, 877
RseI CAYNNNNRTG 1 cut(s) 866
SalI GTCGAC 1 cut(s) 41
SaqAI TTAA 2 cut(s) 404, 744
Sau3AI GATC 3 cut(s) 177, 671, 739
Sau96I GGNCC 1 cut(s) 326
ScaI AGTACT 1 cut(s) 878
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 6 cut(s) 38, 209, 308, 484, 731, 1042
SduI GDGCHC 2 cut(s) 68, 490
SfcI CTRYAG 2 cut(s) 793, 928
SgrAI CRCCGGYG 1 cut(s) 159
SmiMI CAYNNNNRTG 1 cut(s) 866
SmlI CTYRAG 2 cut(s) 693, 1035
SmoI CTYRAG 2 cut(s) 693, 1035
SphI GCATGC 1 cut(s) 910
Sse9I AATT 2 cut(s) 372, 595
SseBI AGGCCT 1 cut(s) 726
StuI AGGCCT 1 cut(s) 726
StyD4I CCNGG 6 cut(s) 36, 207, 306, 482, 729, 1040
StyI CCWWGG 5 cut(s) 87, 225, 604, 640, 957
TaaI ACNGT 2 cut(s) 291, 417
TaiI ACGT 2 cut(s) 176, 207
TaqI TCGA 2 cut(s) 42, 761
TasI AATT 2 cut(s) 372, 595
TatI WGTACW 3 cut(s) 412, 820, 876
TauI GCSGC 6 cut(s) 8, 150, 153, 156, 159, 304
TfiI GAWTC 2 cut(s) 361, 526
Tru1I TTAA 2 cut(s) 404, 744
Tru9I TTAA 2 cut(s) 404, 744
TscAI CASTG 1 cut(s) 477
TseFI GTSAC 1 cut(s) 583
TseI GCWGC 4 cut(s) 443, 927, 930, 1059
Tsp45I GTSAC 1 cut(s) 583
TspDTI ATGAA 4 cut(s) 374, 884, 984, 1061
TspRI CASTG 1 cut(s) 477
Van91I CCANNNNNTGG 2 cut(s) 208, 921
XapI RAATTY 1 cut(s) 595
XceI RCATGY 1 cut(s) 910
XmiI GTMKAC 1 cut(s) 42
ZrmI AGTACT 1 cut(s) 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.