MD08G1174100.v1.1

DnaJ domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011707
Physical Location & Seq
Reverse (-)
21141166 .. 21143610
2445 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1174100.v1.1.491

Sequence Viewer

Length: 807 bp
ATGAGCAATTTGAGAACCACGTGTAGGCCGCACACGGTCTTCACCTCCTTCGTGTATTGCAGATACCAAGCTCGCTTCAGCTCTAGGGTTTCGTTTCGAAACCCTAATTGCAGCCCTCGCTTTCCGCCGGTGTCCGATTTCGCGAGATTGAATTGGGGCGGGTCGTGGTTCCGGGTCAATCAGAGACGGAGCGTGATTCGGGCTTCCAGTTGGGACCACCCTAAATCTCCCTACGAAACTCTTGAATTAGAGAGGGACGCTGACGATGACCAGATAAAGATTGCTTACAGACGGCTGGCCAAGTTTTATCATCCGGATGTTTACGATGGTAGAGGGACTCTAGAGGAGGGGGAAACAGCTGAAGCTAGATTTATTAAGATTCAAGCTGCTTATGAGTTGCTCATGGATGACGAGAAGAGGGGACAATATGATAGGGATAACCGACTCAACCCAATGAAAGCATCTCAGGCATGGATGGAATGGCTAATTAAAAAGCGAAAAGCTTTTGATCAGCGAGGTGATATGGCAATAGCAGCTTGGCAAGAGCAACAGCAGCGTGAGATGAATATTCGCGCGCGTCGACTTTCTCGTTCCAAGGTTGACCCAGATGAAGAGAAGAGAATCCTGGCAAGAGAAAAAAAGGCCTCGGCAGAGTATTTCTCCAGTACCCTCAAGCGACACACGCTAGTCTTAAAGAAAAGGGACCTAATGCGAAGGAAAGCAGAGGAAGACAAGAAAAAGCTCATCGGTCAGCTTTTAGCCGCAGAGGGACTTGAGCTCGACACAGAGGATGAAGAAAAACGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

269

Amino Acids

31.82

Weight (kDa)

9.6

Isoelectric Point (pI)

48.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 77 - 144 5.2e-19 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011707)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77930 AT1G77930 AT1G77930
fragaria_vesca FvH4_5g38391 FvH4_5g38391
malus_domestica MD08G1174100.v1.1 MD15G1360500.v1.1 MD15G1360600.v1.1
prunus_persica Prupe.1G508000_v2.0.a1
pyrus_communis pycom15g32240
rosa_chinensis RchiOBHm_Chr7g0240411
rosa_laevigata RLG00000000679
rosa_roxburghii Rroxscaffold_3G00220140
rosa_rugosa Rorug07G0335800
rosa_samantha Rh7AG490000 Rh7BG462900 Rh7CG506300 Rh7DG473900
rosa_wichuraiana Rw7G040990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 580
AccII CGCG 4 cut(s) 143, 573, 575, 577
AccIII TCCGGA 1 cut(s) 313
AciI CCGC 4 cut(s) 29, 125, 159, 762
AcoI YGGCCR 1 cut(s) 297
AcuI CTGAAG 2 cut(s) 61, 381
AcvI CACGTG 1 cut(s) 21
AfaI GTAC 1 cut(s) 667
AfiI CCNNNNNNNGG 1 cut(s) 24
AflIII ACRYGT 1 cut(s) 20
AgsI TTSAA 3 cut(s) 151, 245, 383
AjnI CCWGG 1 cut(s) 624
Alw21I GWGCWC 1 cut(s) 780
Alw26I GTCTC 1 cut(s) 178
Aor13HI TCCGGA 1 cut(s) 313
AoxI GGCC 3 cut(s) 26, 297, 642
ApeKI GCWGC 4 cut(s) 111, 386, 533, 553
AspLEI GCGC 2 cut(s) 575, 577
AspS9I GGNCC 2 cut(s) 214, 703
AsuC2I CCSGG 1 cut(s) 173
AsuHPI GGTGA 2 cut(s) 34, 530
AsuII TTCGAA 1 cut(s) 97
AvaII GGWCC 2 cut(s) 214, 703
BalI TGGCCA 1 cut(s) 299
BanII GRGCYC 1 cut(s) 780
BbrPI CACGTG 1 cut(s) 21
BbsI GAAGAC 2 cut(s) 31, 735
Bbv12I GWGCWC 1 cut(s) 780
BbvI GCAGC 4 cut(s) 123, 373, 545, 565
BccI CCATC 2 cut(s) 320, 469
BceAI ACGGC 1 cut(s) 308
BciT130I CCWGG 1 cut(s) 626
BclI TGATCA 1 cut(s) 508
BcnI CCSGG 1 cut(s) 173
BcoDI GTCTC 1 cut(s) 178
BfaI CTAG 4 cut(s) 84, 341, 366, 686
BisI GCNGC 6 cut(s) 29, 112, 387, 534, 554, 762
BlsI GCNGC 6 cut(s) 30, 113, 388, 535, 555, 763
Bme1390I CCNGG 2 cut(s) 173, 626
Bme18I GGWCC 2 cut(s) 214, 703
BmgT120I GGNCC 2 cut(s) 214, 703
BmiI GGNNCC 3 cut(s) 170, 215, 704
BmrFI CCNGG 2 cut(s) 173, 626
BmsI GCATC 1 cut(s) 470
BpiI GAAGAC 2 cut(s) 31, 735
BplI GAGNNNNNCTC 2 cut(s) 644, 676
BpmI CTGGAG 1 cut(s) 646
Bpu14I TTCGAA 1 cut(s) 97
BpuEI CTTGAG 2 cut(s) 656, 794
BpuMI CCSGG 1 cut(s) 173
BsaAI YACGTR 1 cut(s) 21
BsaJI CCNNGG 2 cut(s) 594, 645
BsaWI WCCGGW 1 cut(s) 313
Bsc4I CCNNNNNNNGG 1 cut(s) 24
Bse118I RCCGGY 1 cut(s) 127
Bse1I ACTGG 2 cut(s) 207, 663
BseAI TCCGGA 1 cut(s) 313
BseBI CCWGG 1 cut(s) 626
BseDI CCNNGG 2 cut(s) 594, 645
BseGI GGATG 5 cut(s) 310, 322, 412, 480, 796
BseLI CCNNNNNNNGG 1 cut(s) 24
BseMII CTCAG 1 cut(s) 479
BseNI ACTGG 2 cut(s) 207, 663
BsePI GCGCGC 1 cut(s) 573
BseRI GAGGAG 1 cut(s) 359
BseXI GCAGC 4 cut(s) 123, 373, 545, 565
Bsh1236I CGCG 4 cut(s) 143, 573, 575, 577
BshFI GGCC 3 cut(s) 28, 299, 644
BsiHKAI GWGCWC 1 cut(s) 780
BsiSI CCGG 3 cut(s) 128, 172, 314
BslFI GGGAC 6 cut(s) 227, 269, 349, 435, 716, 783
BslI CCNNNNNNNGG 1 cut(s) 24
BsmAI GTCTC 1 cut(s) 178
BsmBI CGTCTC 1 cut(s) 178
BsmFI GGGAC 6 cut(s) 227, 269, 349, 435, 716, 783
BsnI GGCC 3 cut(s) 28, 299, 644
Bsp119I TTCGAA 1 cut(s) 97
Bsp1286I GDGCHC 1 cut(s) 780
Bsp13I TCCGGA 1 cut(s) 313
Bsp143I GATC 1 cut(s) 508
Bsp68I TCGCGA 1 cut(s) 143
BspACI CCGC 4 cut(s) 29, 125, 159, 762
BspANI GGCC 3 cut(s) 28, 299, 644
BspCNI CTCAG 1 cut(s) 478
BspEI TCCGGA 1 cut(s) 313
BspFNI CGCG 4 cut(s) 143, 573, 575, 577
BspLI GGNNCC 3 cut(s) 170, 215, 704
BspT104I TTCGAA 1 cut(s) 97
BsrFI RCCGGY 1 cut(s) 127
BsrI ACTGG 2 cut(s) 207, 663
BssAI RCCGGY 1 cut(s) 127
BssECI CCNNGG 2 cut(s) 594, 645
BssHII GCGCGC 1 cut(s) 573
BssMI GATC 1 cut(s) 508
BssT1I CCWWGG 1 cut(s) 594
Bst2UI CCWGG 1 cut(s) 626
Bst4CI ACNGT 2 cut(s) 37, 804
Bst6I CTCTTC 3 cut(s) 410, 606, 611
BstBAI YACGTR 1 cut(s) 21
BstBI TTCGAA 1 cut(s) 97
BstC8I GCNNGC 3 cut(s) 73, 297, 575
BstDEI CTNAG 1 cut(s) 465
BstF5I GGATG 5 cut(s) 310, 322, 412, 480, 796
BstFNI CGCG 4 cut(s) 143, 573, 575, 577
BstHHI GCGC 2 cut(s) 575, 577
BstKTI GATC 1 cut(s) 511
BstMAI GTCTC 1 cut(s) 178
BstMBI GATC 1 cut(s) 508
BstMWI GCNNNNNNNGC 5 cut(s) 117, 467, 533, 553, 682
BstNI CCWGG 1 cut(s) 626
BstSCI CCNGG 2 cut(s) 171, 624
BstUI CGCG 4 cut(s) 143, 573, 575, 577
BstV1I GCAGC 4 cut(s) 123, 373, 545, 565
BstV2I GAAGAC 2 cut(s) 31, 735
BsuRI GGCC 3 cut(s) 28, 299, 644
BtsCI GGATG 5 cut(s) 310, 322, 412, 480, 796
BtuMI TCGCGA 1 cut(s) 143
Cac8I GCNNGC 3 cut(s) 73, 297, 575
CfoI GCGC 2 cut(s) 575, 577
Cfr10I RCCGGY 1 cut(s) 127
Cfr13I GGNCC 2 cut(s) 214, 703
CseI GACGC 2 cut(s) 266, 566
Csp6I GTAC 1 cut(s) 666
CviAII CATG 2 cut(s) 403, 471
CviQI GTAC 1 cut(s) 666
DdeI CTNAG 1 cut(s) 465
DpnI GATC 1 cut(s) 510
DpnII GATC 1 cut(s) 508
EaeI YGGCCR 1 cut(s) 297
Eam1104I CTCTTC 3 cut(s) 410, 606, 611
EarI CTCTTC 3 cut(s) 410, 606, 611
EciI GGCGGA 1 cut(s) 114
Ecl136II GAGCTC 1 cut(s) 778
Eco130I CCWWGG 1 cut(s) 594
Eco147I AGGCCT 1 cut(s) 644
Eco24I GRGCYC 1 cut(s) 780
Eco47I GGWCC 2 cut(s) 214, 703
Eco53kI GAGCTC 1 cut(s) 778
Eco57I CTGAAG 2 cut(s) 61, 381
Eco72I CACGTG 1 cut(s) 21
EcoICRI GAGCTC 1 cut(s) 778
EcoO109I RGGNCCY 1 cut(s) 703
EcoRII CCWGG 1 cut(s) 624
EcoT14I CCWWGG 1 cut(s) 594
EcoT38I GRGCYC 1 cut(s) 780
ErhI CCWWGG 1 cut(s) 594
Esp3I CGTCTC 1 cut(s) 178
FaeI CATG 2 cut(s) 406, 474
FaiI YATR 5 cut(s) 393, 404, 429, 472, 524
FaqI GGGAC 6 cut(s) 227, 269, 349, 435, 716, 783
FatI CATG 2 cut(s) 402, 470
FauI CCCGC 1 cut(s) 152
FbaI TGATCA 1 cut(s) 508
FblI GTMKAC 1 cut(s) 580
Fnu4HI GCNGC 6 cut(s) 29, 112, 387, 534, 554, 762
FokI GGATG 5 cut(s) 297, 329, 419, 487, 803
FriOI GRGCYC 1 cut(s) 780
Fsp4HI GCNGC 6 cut(s) 29, 112, 387, 534, 554, 762
FspBI CTAG 4 cut(s) 84, 341, 366, 686
GlaI GCGC 2 cut(s) 574, 576
GluI GCNGC 6 cut(s) 29, 112, 387, 534, 554, 762
GsuI CTGGAG 1 cut(s) 646
HaeIII GGCC 3 cut(s) 28, 299, 644
HapII CCGG 3 cut(s) 128, 172, 314
HgaI GACGC 2 cut(s) 266, 566
HhaI GCGC 2 cut(s) 575, 577
Hin1II CATG 2 cut(s) 406, 474
Hin6I GCGC 2 cut(s) 573, 575
HinP1I GCGC 2 cut(s) 573, 575
HincII GTYRAC 2 cut(s) 581, 601
HindII GTYRAC 2 cut(s) 581, 601
HindIII AAGCTT 1 cut(s) 501
HinfI GANTC 5 cut(s) 196, 337, 379, 444, 621
HpaII CCGG 3 cut(s) 128, 172, 314
HphI GGTGA 2 cut(s) 34, 530
Hpy166II GTNNAC 3 cut(s) 322, 581, 601
Hpy188I TCNGA 2 cut(s) 136, 183
Hpy188III TCNNGA 4 cut(s) 142, 242, 314, 341
Hpy8I GTNNAC 3 cut(s) 322, 581, 601
Hpy99I CGWCG 1 cut(s) 582
HpyAV CCTTC 2 cut(s) 58, 708
HpyCH4III ACNGT 2 cut(s) 37, 804
HpyCH4IV ACGT 1 cut(s) 20
HpyCH4V TGCA 2 cut(s) 60, 111
HpyF10VI GCNNNNNNNGC 5 cut(s) 117, 467, 533, 553, 682
HpyF3I CTNAG 1 cut(s) 465
HpySE526I ACGT 1 cut(s) 20
Hsp92II CATG 2 cut(s) 406, 474
HspAI GCGC 2 cut(s) 573, 575
Kpn2I TCCGGA 1 cut(s) 313
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 1 cut(s) 508
LmnI GCTCC 1 cut(s) 189
Lsp1109I GCAGC 4 cut(s) 123, 373, 545, 565
LweI GCATC 1 cut(s) 470
MaeI CTAG 4 cut(s) 84, 341, 366, 686
MaeII ACGT 1 cut(s) 20
MalI GATC 1 cut(s) 510
MauBI CGCGCGCG 1 cut(s) 573
MboI GATC 1 cut(s) 508
MboII GAAGA 6 cut(s) 31, 427, 623, 628, 740, 806
MhlI GDGCHC 1 cut(s) 780
MlsI TGGCCA 1 cut(s) 299
MluCI AATT 5 cut(s) 7, 106, 151, 245, 486
MluNI TGGCCA 1 cut(s) 299
MlyI GAGTC 2 cut(s) 331, 438
Mox20I TGGCCA 1 cut(s) 299
MroI TCCGGA 1 cut(s) 313
MscI TGGCCA 1 cut(s) 299
MseI TTAA 3 cut(s) 375, 489, 692
MslI CAYNNNNRTG 1 cut(s) 315
Msp20I TGGCCA 1 cut(s) 299
MspA1I CMGCKG 1 cut(s) 359
MspI CCGG 3 cut(s) 128, 172, 314
MspR9I CCNGG 2 cut(s) 173, 626
MvaI CCWGG 1 cut(s) 626
MvnI CGCG 4 cut(s) 143, 573, 575, 577
MwoI GCNNNNNNNGC 5 cut(s) 117, 467, 533, 553, 682
NciI CCSGG 1 cut(s) 173
NdeII GATC 1 cut(s) 508
NlaIII CATG 2 cut(s) 406, 474
NlaIV GGNNCC 3 cut(s) 170, 215, 704
NmeAIII GCCGAG 1 cut(s) 626
NruI TCGCGA 1 cut(s) 143
NspV TTCGAA 1 cut(s) 97
PauI GCGCGC 1 cut(s) 573
PceI AGGCCT 1 cut(s) 644
PcsI WCGNNNNNNNCGW 2 cut(s) 577, 586
PfeI GAWTC 3 cut(s) 196, 379, 621
PkrI GCNGC 6 cut(s) 30, 113, 388, 535, 555, 763
PleI GAGTC 2 cut(s) 331, 438
PmaCI CACGTG 1 cut(s) 21
PmlI CACGTG 1 cut(s) 21
PpsI GAGTC 2 cut(s) 331, 438
Ppu21I YACGTR 1 cut(s) 21
PpuMI RGGWCCY 1 cut(s) 703
Psp124BI GAGCTC 1 cut(s) 780
Psp5II RGGWCCY 1 cut(s) 703
Psp6I CCWGG 1 cut(s) 624
PspCI CACGTG 1 cut(s) 21
PspGI CCWGG 1 cut(s) 624
PspN4I GGNNCC 3 cut(s) 170, 215, 704
PspPI GGNCC 2 cut(s) 214, 703
PspPPI RGGWCCY 1 cut(s) 703
PteI GCGCGC 1 cut(s) 573
PvuII CAGCTG 1 cut(s) 359
RruI TCGCGA 1 cut(s) 143
RsaI GTAC 1 cut(s) 667
RsaNI GTAC 1 cut(s) 666
RseI CAYNNNNRTG 1 cut(s) 315
SacI GAGCTC 1 cut(s) 780
SalI GTCGAC 1 cut(s) 579
SaqAI TTAA 3 cut(s) 375, 489, 692
SatI GCNGC 6 cut(s) 29, 112, 387, 534, 554, 762
Sau3AI GATC 1 cut(s) 508
Sau96I GGNCC 2 cut(s) 214, 703
SchI GAGTC 2 cut(s) 331, 438
ScrFI CCNGG 2 cut(s) 173, 626
SduI GDGCHC 1 cut(s) 780
SfaNI GCATC 1 cut(s) 470
SfuI TTCGAA 1 cut(s) 97
SgrAI CRCCGGYG 1 cut(s) 127
SinI GGWCC 2 cut(s) 214, 703
SmiMI CAYNNNNRTG 1 cut(s) 315
SmlI CTYRAG 2 cut(s) 671, 773
SmoI CTYRAG 2 cut(s) 671, 773
Sse9I AATT 5 cut(s) 7, 106, 151, 245, 486
SseBI AGGCCT 1 cut(s) 644
SsiI CCGC 4 cut(s) 29, 125, 159, 762
SspI AATATT 1 cut(s) 568
SspMI CTAG 4 cut(s) 84, 341, 366, 686
SstI GAGCTC 1 cut(s) 780
StuI AGGCCT 1 cut(s) 644
StyD4I CCNGG 2 cut(s) 171, 624
StyI CCWWGG 1 cut(s) 594
TaaI ACNGT 2 cut(s) 37, 804
TaiI ACGT 1 cut(s) 23
TaqI TCGA 3 cut(s) 97, 580, 780
TaqII GACCGA 1 cut(s) 737
TasI AATT 5 cut(s) 7, 106, 151, 245, 486
TauI GCSGC 2 cut(s) 31, 764
TfiI GAWTC 3 cut(s) 196, 379, 621
Tru1I TTAA 3 cut(s) 375, 489, 692
Tru9I TTAA 3 cut(s) 375, 489, 692
TseI GCWGC 4 cut(s) 111, 386, 533, 553
TspDTI ATGAA 4 cut(s) 470, 578, 624, 807
TspGWI ACGGA 1 cut(s) 202
VpaK11BI GGWCC 2 cut(s) 214, 703
XbaI TCTAGA 1 cut(s) 340
XmiI GTMKAC 1 cut(s) 580
XspI CTAG 4 cut(s) 84, 341, 366, 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.