MD08G1176300.v1.1

Membrane-anchored ubiquitin-fold protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0016524
Physical Location & Seq
Reverse (-)
21390530 .. 21393422
2893 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1176300.v1.1.491

Sequence Viewer

Length: 360 bp
ATGGCGGCAGGTCAAGATTTGATTGAGCTGAAATTCAGGCTATTTGATGGGACCGACATAGGACCTCAGAAGTATAGCCCATCTACCACTGTACAATCACTCAAGGAGAAAATACTTGCACAATGGCCTAAAGAGAAAGAAAATGGTCCAAGGACAATAAATGATTTGAAGCTTATTAATGCGGGAAAGATACTGGAAAACAACAGGACACTTGCTGATTCCAGAAATCCAGTTGGTGAACTCCCTGGAAGTCTCATCACTATGCACGTGGTTGTGCGCCCTCCTATTGCAGACAAAAAGAATGATAAATTGATGAATGATTCTCCAGAGCAGCCTCGATGTTCATGTTCAATATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rad60-SLD_2

Protein Analysis

120

Amino Acids

13.21

Weight (kDa)

8.6

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD_2 PF13881 6 - 117 8.3e-44 Ubiquitin-2 like Rad60 SUMO-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016524)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22050
fragaria_vesca FvH4_5g38170
malus_domestica MD08G1176300.v1.1 MD15G1362100.v1.1
prunus_persica Prupe.1G510100_v2.0.a1
pyrus_communis pycom08g15130 pycom15g32470
rosa_chinensis RchiOBHm_Chr7g0240171
rosa_laevigata RLG00000000693
rosa_multiflora Rmu_sc0005008.1_g000005
rosa_roxburghii Rroxscaffold_3G00220350
rosa_rugosa Rorug07G0333900
rosa_samantha Rh7CG504200
rosa_wichuraiana Rw7G040850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 5, 182
AcsI RAATTY 1 cut(s) 32
AcvI CACGTG 1 cut(s) 268
AfaI GTAC 1 cut(s) 93
AgsI TTSAA 2 cut(s) 169, 351
AjnI CCWGG 1 cut(s) 244
AluBI AGCT 2 cut(s) 28, 172
AluI AGCT 2 cut(s) 28, 172
Alw26I GTCTC 1 cut(s) 257
AoxI GGCC 1 cut(s) 125
ApeKI GCWGC 1 cut(s) 331
ApoI RAATTY 1 cut(s) 32
AseI ATTAAT 1 cut(s) 177
AspLEI GCGC 1 cut(s) 279
AspS9I GGNCC 3 cut(s) 51, 62, 146
AsuHPI GGTGA 1 cut(s) 248
AvaII GGWCC 3 cut(s) 51, 62, 146
BbrPI CACGTG 1 cut(s) 268
BbvI GCAGC 1 cut(s) 343
BccI CCATC 2 cut(s) 41, 88
BciT130I CCWGG 1 cut(s) 246
BcoDI GTCTC 1 cut(s) 257
BisI GCNGC 2 cut(s) 6, 332
BlsI GCNGC 2 cut(s) 7, 333
Bme1390I CCNGG 1 cut(s) 246
Bme18I GGWCC 3 cut(s) 51, 62, 146
BmgT120I GGNCC 3 cut(s) 51, 62, 146
BmiI GGNNCC 1 cut(s) 52
BmrFI CCNGG 1 cut(s) 246
BpmI CTGGAG 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 86
BsaAI YACGTR 1 cut(s) 268
BsaJI CCNNGG 2 cut(s) 149, 244
Bse1I ACTGG 2 cut(s) 198, 230
BseBI CCWGG 1 cut(s) 246
BseDI CCNNGG 2 cut(s) 149, 244
BseMII CTCAG 1 cut(s) 80
BseNI ACTGG 2 cut(s) 198, 230
BseXI GCAGC 1 cut(s) 343
BshFI GGCC 1 cut(s) 127
BslFI GGGAC 1 cut(s) 64
BsmAI GTCTC 1 cut(s) 257
BsmFI GGGAC 1 cut(s) 64
BsnI GGCC 1 cut(s) 127
Bsp1407I TGTACA 1 cut(s) 91
BspACI CCGC 2 cut(s) 5, 182
BspANI GGCC 1 cut(s) 127
BspCNI CTCAG 1 cut(s) 79
BspLI GGNNCC 1 cut(s) 52
BsrGI TGTACA 1 cut(s) 91
BsrI ACTGG 2 cut(s) 198, 230
BssECI CCNNGG 2 cut(s) 149, 244
BssT1I CCWWGG 1 cut(s) 149
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 1 cut(s) 91
BstAUI TGTACA 1 cut(s) 91
BstBAI YACGTR 1 cut(s) 268
BstDEI CTNAG 1 cut(s) 66
BstHHI GCGC 1 cut(s) 279
BstMAI GTCTC 1 cut(s) 257
BstNI CCWGG 1 cut(s) 246
BstSCI CCNGG 1 cut(s) 244
BstV1I GCAGC 1 cut(s) 343
BsuRI GGCC 1 cut(s) 127
BtsIMutI CAGTG 1 cut(s) 87
CfoI GCGC 1 cut(s) 279
Cfr13I GGNCC 3 cut(s) 51, 62, 146
Csp6I GTAC 1 cut(s) 92
CviAII CATG 1 cut(s) 345
CviJI RGCY 6 cut(s) 28, 40, 78, 127, 172, 334
CviKI_1 RGCY 6 cut(s) 28, 40, 78, 127, 172, 334
CviQI GTAC 1 cut(s) 92
DdeI CTNAG 1 cut(s) 66
Eco130I CCWWGG 1 cut(s) 149
Eco47I GGWCC 3 cut(s) 51, 62, 146
Eco72I CACGTG 1 cut(s) 268
EcoO109I RGGNCCY 1 cut(s) 62
EcoRII CCWGG 1 cut(s) 244
EcoT14I CCWWGG 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 149
FaeI CATG 1 cut(s) 348
FaiI YATR 4 cut(s) 59, 75, 263, 346
FaqI GGGAC 1 cut(s) 64
FatI CATG 1 cut(s) 344
FauI CCCGC 1 cut(s) 175
Fnu4HI GCNGC 2 cut(s) 6, 332
Fsp4HI GCNGC 2 cut(s) 6, 332
GlaI GCGC 1 cut(s) 278
GluI GCNGC 2 cut(s) 6, 332
GsuI CTGGAG 1 cut(s) 309
HaeIII GGCC 1 cut(s) 127
HhaI GCGC 1 cut(s) 279
Hin1II CATG 1 cut(s) 348
Hin6I GCGC 1 cut(s) 277
HinP1I GCGC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 2 cut(s) 218, 320
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 1 cut(s) 239
Hpy188I TCNGA 1 cut(s) 69
Hpy188III TCNNGA 3 cut(s) 14, 222, 326
Hpy8I GTNNAC 1 cut(s) 239
HpyCH4III ACNGT 1 cut(s) 91
HpyCH4IV ACGT 1 cut(s) 267
HpyCH4V TGCA 3 cut(s) 119, 265, 290
HpyF3I CTNAG 1 cut(s) 66
HpySE526I ACGT 1 cut(s) 267
Hsp92II CATG 1 cut(s) 348
HspAI GCGC 1 cut(s) 277
LpnPI CCDG 8 cut(s) 22, 179, 190, 231, 235, 243, 258, 339
Lsp1109I GCAGC 1 cut(s) 343
MaeII ACGT 1 cut(s) 267
MluCI AATT 2 cut(s) 32, 308
MnlI CCTC 3 cut(s) 75, 291, 345
MseI TTAA 1 cut(s) 177
MslI CAYNNNNRTG 1 cut(s) 260
MspR9I CCNGG 1 cut(s) 246
MvaI CCWGG 1 cut(s) 246
NlaIII CATG 1 cut(s) 348
NlaIV GGNNCC 1 cut(s) 52
PfeI GAWTC 2 cut(s) 218, 320
PkrI GCNGC 2 cut(s) 7, 333
PmaCI CACGTG 1 cut(s) 268
PmlI CACGTG 1 cut(s) 268
Ppu21I YACGTR 1 cut(s) 268
PpuMI RGGWCCY 1 cut(s) 62
PshBI ATTAAT 1 cut(s) 177
Psp5II RGGWCCY 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 244
PspCI CACGTG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 1 cut(s) 52
PspPI GGNCC 3 cut(s) 51, 62, 146
PspPPI RGGWCCY 1 cut(s) 62
RsaI GTAC 1 cut(s) 93
RsaNI GTAC 1 cut(s) 92
RseI CAYNNNNRTG 1 cut(s) 260
SaqAI TTAA 1 cut(s) 177
SatI GCNGC 2 cut(s) 6, 332
Sau96I GGNCC 3 cut(s) 51, 62, 146
ScrFI CCNGG 1 cut(s) 246
SetI ASST 5 cut(s) 13, 30, 67, 174, 270
SinI GGWCC 3 cut(s) 51, 62, 146
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
Sse9I AATT 2 cut(s) 32, 308
SsiI CCGC 2 cut(s) 5, 182
SspI AATATT 1 cut(s) 354
StyD4I CCNGG 1 cut(s) 244
StyI CCWWGG 1 cut(s) 149
TaaI ACNGT 1 cut(s) 91
TaiI ACGT 1 cut(s) 270
TaqI TCGA 1 cut(s) 337
TaqII GACCGA 1 cut(s) 68
TasI AATT 2 cut(s) 32, 308
TatI WGTACW 1 cut(s) 91
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 218, 320
Tru1I TTAA 1 cut(s) 177
Tru9I TTAA 1 cut(s) 177
TscAI CASTG 1 cut(s) 94
TseI GCWGC 1 cut(s) 331
TspDTI ATGAA 2 cut(s) 329, 333
TspRI CASTG 1 cut(s) 94
VpaK11BI GGWCC 3 cut(s) 51, 62, 146
VspI ATTAAT 1 cut(s) 177
XapI RAATTY 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.