MD15G1362100.v1.1

Membrane-anchored ubiquitin-fold protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016524
Physical Location & Seq
Reverse (-)
43670151 .. 43672926
2776 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1362100.v1.1.491

Sequence Viewer

Length: 360 bp
ATGGCGGCAGGTCAAGATTTGATTGAGCTGAAATTCAGGCTAATTGATGGTACCGACATAGGACCACAGAAGTATAGCCCATCTACCACTGTACAATCACTCAAGGAGAAAATACTGGCACAATGGCCTAAAGAGAAAGAAAATGGTCCAAGGACAATAAATGATTTGAAGCTTATTAATGCGGGAAAGATACTGGAAAACAACAGGACACTTGCTGATTCCAGAAATCCAGTTGGAGAACTACCAGGAGGTCTCATCACTATGCATGTGGTTGTGCGTCCTCCGATTGCAGACAAAAAGAATGATAAGTTGATGAATGATTCTCCAGAGCAGCCTCGATGTTCATGTTCAATATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rad60-SLD_2

Protein Analysis

120

Amino Acids

13.15

Weight (kDa)

8.6

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD_2 PF13881 6 - 117 5.1e-44 Ubiquitin-2 like Rad60 SUMO-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016524)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22050
fragaria_vesca FvH4_5g38170
malus_domestica MD08G1176300.v1.1 MD15G1362100.v1.1
prunus_persica Prupe.1G510100_v2.0.a1
pyrus_communis pycom08g15130 pycom15g32470
rosa_chinensis RchiOBHm_Chr7g0240171
rosa_laevigata RLG00000000693
rosa_multiflora Rmu_sc0005008.1_g000005
rosa_roxburghii Rroxscaffold_3G00220350
rosa_rugosa Rorug07G0333900
rosa_samantha Rh7CG504200
rosa_wichuraiana Rw7G040850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 50
AccB1I GGYRCC 1 cut(s) 50
AciI CCGC 2 cut(s) 5, 182
AcsI RAATTY 1 cut(s) 32
AfaI GTAC 2 cut(s) 52, 93
AgsI TTSAA 2 cut(s) 169, 351
AjnI CCWGG 1 cut(s) 244
AluBI AGCT 2 cut(s) 28, 172
AluI AGCT 2 cut(s) 28, 172
Alw26I GTCTC 1 cut(s) 257
AoxI GGCC 1 cut(s) 125
ApeKI GCWGC 1 cut(s) 331
ApoI RAATTY 1 cut(s) 32
AseI ATTAAT 1 cut(s) 177
Asp718I GGTACC 1 cut(s) 50
AspS9I GGNCC 2 cut(s) 62, 146
AvaII GGWCC 2 cut(s) 62, 146
BanI GGYRCC 1 cut(s) 50
BbvI GCAGC 1 cut(s) 343
BccI CCATC 2 cut(s) 41, 88
BciT130I CCWGG 1 cut(s) 246
BcoDI GTCTC 1 cut(s) 257
BisI GCNGC 2 cut(s) 6, 332
BlsI GCNGC 2 cut(s) 7, 333
Bme1390I CCNGG 1 cut(s) 246
Bme18I GGWCC 2 cut(s) 62, 146
BmgT120I GGNCC 2 cut(s) 62, 146
BmiI GGNNCC 1 cut(s) 52
BmrFI CCNGG 1 cut(s) 246
BpmI CTGGAG 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 86
BsaI GGTCTC 1 cut(s) 257
BsaJI CCNNGG 1 cut(s) 149
Bse1I ACTGG 3 cut(s) 120, 198, 230
BseBI CCWGG 1 cut(s) 246
BseDI CCNNGG 1 cut(s) 149
BseNI ACTGG 3 cut(s) 120, 198, 230
BseXI GCAGC 1 cut(s) 343
BshFI GGCC 1 cut(s) 127
BshNI GGYRCC 1 cut(s) 50
BsmAI GTCTC 1 cut(s) 257
BsnI GGCC 1 cut(s) 127
Bso31I GGTCTC 1 cut(s) 257
Bsp1407I TGTACA 1 cut(s) 91
BspACI CCGC 2 cut(s) 5, 182
BspANI GGCC 1 cut(s) 127
BspLI GGNNCC 1 cut(s) 52
BspT107I GGYRCC 1 cut(s) 50
BspTNI GGTCTC 1 cut(s) 257
BsrGI TGTACA 1 cut(s) 91
BsrI ACTGG 3 cut(s) 120, 198, 230
BssECI CCNNGG 1 cut(s) 149
BssT1I CCWWGG 1 cut(s) 149
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 1 cut(s) 91
BstAUI TGTACA 1 cut(s) 91
BstMAI GTCTC 1 cut(s) 257
BstNI CCWGG 1 cut(s) 246
BstNSI RCATGY 1 cut(s) 269
BstSCI CCNGG 1 cut(s) 244
BstV1I GCAGC 1 cut(s) 343
BsuRI GGCC 1 cut(s) 127
BtsIMutI CAGTG 1 cut(s) 87
Cfr13I GGNCC 2 cut(s) 62, 146
CseI GACGC 1 cut(s) 266
Csp6I GTAC 2 cut(s) 51, 92
CviAII CATG 2 cut(s) 266, 345
CviJI RGCY 6 cut(s) 28, 40, 78, 127, 172, 334
CviKI_1 RGCY 6 cut(s) 28, 40, 78, 127, 172, 334
CviQI GTAC 2 cut(s) 51, 92
Eco130I CCWWGG 1 cut(s) 149
Eco31I GGTCTC 1 cut(s) 257
Eco47I GGWCC 2 cut(s) 62, 146
EcoRII CCWGG 1 cut(s) 244
EcoT14I CCWWGG 1 cut(s) 149
EcoT22I ATGCAT 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 149
FaeI CATG 2 cut(s) 269, 348
FaiI YATR 5 cut(s) 59, 75, 263, 267, 346
FatI CATG 2 cut(s) 265, 344
FauI CCCGC 1 cut(s) 175
Fnu4HI GCNGC 2 cut(s) 6, 332
Fsp4HI GCNGC 2 cut(s) 6, 332
GluI GCNGC 2 cut(s) 6, 332
GsuI CTGGAG 1 cut(s) 309
HaeIII GGCC 1 cut(s) 127
HgaI GACGC 1 cut(s) 266
Hin1II CATG 2 cut(s) 269, 348
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 2 cut(s) 218, 320
Hpy188I TCNGA 1 cut(s) 285
Hpy188III TCNNGA 3 cut(s) 14, 222, 326
HpyCH4III ACNGT 1 cut(s) 91
HpyCH4V TGCA 2 cut(s) 265, 290
Hsp92II CATG 2 cut(s) 269, 348
KpnI GGTACC 1 cut(s) 54
LpnPI CCDG 9 cut(s) 22, 101, 179, 190, 231, 235, 243, 258, 339
Lsp1109I GCAGC 1 cut(s) 343
MluCI AATT 2 cut(s) 32, 42
MmeI TCCRAC 1 cut(s) 214
MnlI CCTC 3 cut(s) 242, 291, 345
Mph1103I ATGCAT 1 cut(s) 267
MseI TTAA 1 cut(s) 177
MslI CAYNNNNRTG 1 cut(s) 260
MspR9I CCNGG 1 cut(s) 246
MvaI CCWGG 1 cut(s) 246
NlaIII CATG 2 cut(s) 269, 348
NlaIV GGNNCC 1 cut(s) 52
NsiI ATGCAT 1 cut(s) 267
NspI RCATGY 1 cut(s) 269
PfeI GAWTC 2 cut(s) 218, 320
PkrI GCNGC 2 cut(s) 7, 333
PshBI ATTAAT 1 cut(s) 177
Psp6I CCWGG 1 cut(s) 244
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 1 cut(s) 52
PspPI GGNCC 2 cut(s) 62, 146
RsaI GTAC 2 cut(s) 52, 93
RsaNI GTAC 2 cut(s) 51, 92
RseI CAYNNNNRTG 1 cut(s) 260
SaqAI TTAA 1 cut(s) 177
SatI GCNGC 2 cut(s) 6, 332
Sau96I GGNCC 2 cut(s) 62, 146
ScrFI CCNGG 1 cut(s) 246
SetI ASST 4 cut(s) 13, 30, 174, 253
SinI GGWCC 2 cut(s) 62, 146
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
Sse9I AATT 2 cut(s) 32, 42
SsiI CCGC 2 cut(s) 5, 182
SspI AATATT 1 cut(s) 354
StyD4I CCNGG 1 cut(s) 244
StyI CCWWGG 1 cut(s) 149
TaaI ACNGT 1 cut(s) 91
TaqI TCGA 1 cut(s) 337
TasI AATT 2 cut(s) 32, 42
TatI WGTACW 1 cut(s) 91
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 218, 320
Tru1I TTAA 1 cut(s) 177
Tru9I TTAA 1 cut(s) 177
TscAI CASTG 1 cut(s) 94
TseI GCWGC 1 cut(s) 331
TspDTI ATGAA 2 cut(s) 329, 333
TspRI CASTG 1 cut(s) 94
VpaK11BI GGWCC 2 cut(s) 62, 146
VspI ATTAAT 1 cut(s) 177
XapI RAATTY 1 cut(s) 32
XceI RCATGY 1 cut(s) 269
Zsp2I ATGCAT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.