MD08G1184000.v1.1

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010696
Physical Location & Seq
Reverse (-)
22921142 .. 22936861
15720 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1184000.v1.1.491

Sequence Viewer

Length: 759 bp
ATGGAGATAGAGAGAGAGAAGCATGCTTACCAGGCGAAACTTGCCGAGCAAGCCGAAAGATACGACGAGATGATTGAAGAAATGAAGAAAGTTGCCAAGTTGGATGTGGAATTGAGCGTGGAGGAGCGAAATTTGTTGTCAGTTGGGTATAAGAGTGTGATCGGGGCAAGAAGGGCGTCATGGCGGATACTGTCTTCCATTGAACAAAAGGAAGAGGCCAAGGGTGGTGAACAAAATGTGAAGAGGATAAAGGAGTACAGGCAGAGGGTTGAAGACGAGCTTGCGAAAATTTGCCATGACATACTAACAGTTGTTGATTATCACCTTCTCCCCTCTTCGTCCACTGGGGAATCTACTGTTTTTTACCATAAGATGAAAGGAGATTACTATCGATATCTAGCTGAATTCAAATCTGGTGATGATCGCAAACAAGCTGCTGATCACTCGCTTAAAGCCTATTTGACTGCGGTTGATACTGCTGCCTCTGAGTTGCCCCCAACACATCCAATCAGGCTCGGCCTGGCTCTAAACTTCTCTGTTTTTTACTATGAAATTCTCAACTCCCCGGAGAGGGCGTGTCATCTAGCTAAGAAGGCATTTGATGAGACTTTTGCCGAACTTGACAGCATCGACCAAGAATCTAGCAAGGACAGCACCCTCATTATGCAGCTTCTCAAAGATAATCTCACATTGTGGACCTCAGATCTGCCAGAGGGAGGTGAGAAACTTAAAGTTGAAGAACCCCTTCCAGAGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

253

Amino Acids

28.84

Weight (kDa)

5.18

Isoelectric Point (pI)

52.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 12 - 233 8.9e-104 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010696)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22300 AT1G22300 AT1G22300 AT1G34760 AT1G34760
fragaria_vesca FvH4_5g37150
malus_domestica MD08G1184000.v1.1 MD15G1370500.v1.1
prunus_persica Prupe.1G518200_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0238741
rosa_laevigata RLG00000000816
rosa_multiflora Rmu_sc0010914.1_g000010
rosa_roxburghii Rroxscaffold_3G00221590
rosa_rugosa Rorug07G0323200.1 Rorug07G0323300.1
rosa_samantha Rh7AG477600 Rh7BG448900 Rh7CG495600 Rh7DG462600
rosa_wichuraiana Rw7G039860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 184, 467
AcsI RAATTY 4 cut(s) 130, 288, 404, 552
AcyI GRCGYC 1 cut(s) 176
AdeI CACNNNGTG 1 cut(s) 693
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 3 cut(s) 570, 571, 716
AgsI TTSAA 5 cut(s) 77, 203, 272, 409, 737
AjnI CCWGG 2 cut(s) 30, 519
AluBI AGCT 5 cut(s) 280, 401, 434, 587, 670
AluI AGCT 5 cut(s) 280, 401, 434, 587, 670
Alw26I GTCTC 1 cut(s) 599
AoxI GGCC 2 cut(s) 216, 517
ApeKI GCWGC 3 cut(s) 434, 479, 667
ApoI RAATTY 4 cut(s) 130, 288, 404, 552
Asp700I GAANNNNTTC 1 cut(s) 744
AspS9I GGNCC 1 cut(s) 696
AsuC2I CCSGG 1 cut(s) 566
AsuHPI GGTGA 4 cut(s) 239, 314, 428, 731
AvaII GGWCC 1 cut(s) 696
BarI GAAGNNNNNNTAC 2 cut(s) 11, 43
BbsI GAAGAC 2 cut(s) 186, 279
BbvI GCAGC 3 cut(s) 421, 466, 679
BciT130I CCWGG 2 cut(s) 32, 521
BciVI GTATCC 1 cut(s) 180
BclI TGATCA 1 cut(s) 439
BcnI CCSGG 1 cut(s) 566
BcoDI GTCTC 1 cut(s) 599
BfaI CTAG 3 cut(s) 398, 584, 642
BfuI GTATCC 1 cut(s) 180
BglII AGATCT 1 cut(s) 703
BisI GCNGC 3 cut(s) 435, 480, 668
BlsI GCNGC 3 cut(s) 436, 481, 669
Bme1390I CCNGG 3 cut(s) 32, 521, 566
Bme18I GGWCC 1 cut(s) 696
BmgT120I GGNCC 1 cut(s) 696
BmrFI CCNGG 3 cut(s) 32, 521, 566
BmrI ACTGGG 1 cut(s) 354
BmsI GCATC 1 cut(s) 636
BmuI ACTGGG 1 cut(s) 354
BpiI GAAGAC 2 cut(s) 186, 279
BpuMI CCSGG 1 cut(s) 566
Bsa29I ATCGAT 1 cut(s) 391
BsaBI GATNNNNATC 1 cut(s) 387
BsaHI GRCGYC 1 cut(s) 176
BsaJI CCNNGG 2 cut(s) 219, 564
BsaXI ACNNNNNCTCC 2 cut(s) 560, 590
Bsc4I CCNNNNNNNGG 3 cut(s) 570, 571, 716
Bse1I ACTGG 1 cut(s) 349
Bse8I GATNNNNATC 1 cut(s) 387
BseBI CCWGG 2 cut(s) 32, 521
BseCI ATCGAT 1 cut(s) 391
BseDI CCNNGG 2 cut(s) 219, 564
BseGI GGATG 2 cut(s) 109, 502
BseJI GATNNNNATC 1 cut(s) 387
BseLI CCNNNNNNNGG 3 cut(s) 570, 571, 716
BseMII CTCAG 2 cut(s) 477, 714
BseNI ACTGG 1 cut(s) 349
BseRI GAGGAG 1 cut(s) 137
BseXI GCAGC 3 cut(s) 421, 466, 679
BshFI GGCC 2 cut(s) 218, 519
BshVI ATCGAT 1 cut(s) 391
BsiSI CCGG 1 cut(s) 566
BslI CCNNNNNNNGG 3 cut(s) 570, 571, 716
BsmAI GTCTC 1 cut(s) 599
BsnI GGCC 2 cut(s) 218, 519
Bsp143I GATC 4 cut(s) 159, 421, 439, 703
BspACI CCGC 2 cut(s) 184, 467
BspANI GGCC 2 cut(s) 218, 519
BspCNI CTCAG 2 cut(s) 478, 713
BspDI ATCGAT 1 cut(s) 391
BsrI ACTGG 1 cut(s) 349
BssECI CCNNGG 2 cut(s) 219, 564
BssMI GATC 4 cut(s) 159, 421, 439, 703
BssNI GRCGYC 1 cut(s) 176
BssT1I CCWWGG 1 cut(s) 219
Bst2UI CCWGG 2 cut(s) 32, 521
Bst4CI ACNGT 3 cut(s) 192, 310, 358
Bst6I CTCTTC 3 cut(s) 207, 236, 340
BstACI GRCGYC 1 cut(s) 176
BstC8I GCNNGC 3 cut(s) 24, 51, 282
BstDEI CTNAG 3 cut(s) 486, 588, 700
BstF5I GGATG 2 cut(s) 109, 502
BstKTI GATC 4 cut(s) 162, 424, 442, 706
BstMAI GTCTC 1 cut(s) 599
BstMBI GATC 4 cut(s) 159, 421, 439, 703
BstMWI GCNNNNNNNGC 6 cut(s) 32, 41, 50, 173, 593, 651
BstNI CCWGG 2 cut(s) 32, 521
BstNSI RCATGY 1 cut(s) 26
BstSCI CCNGG 3 cut(s) 30, 519, 564
BstV1I GCAGC 3 cut(s) 421, 466, 679
BstV2I GAAGAC 2 cut(s) 186, 279
BstX2I RGATCY 1 cut(s) 703
BstYI RGATCY 1 cut(s) 703
Bsu15I ATCGAT 1 cut(s) 391
BsuI GTATCC 1 cut(s) 180
BsuRI GGCC 2 cut(s) 218, 519
BsuTUI ATCGAT 1 cut(s) 391
BtsCI GGATG 2 cut(s) 109, 502
BtsIMutI CAGTG 1 cut(s) 342
Cac8I GCNNGC 3 cut(s) 24, 51, 282
Cfr13I GGNCC 1 cut(s) 696
ClaI ATCGAT 1 cut(s) 391
CseI GACGC 1 cut(s) 165
Csp6I GTAC 1 cut(s) 256
CviAII CATG 3 cut(s) 23, 180, 296
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 3 cut(s) 486, 588, 700
DpnI GATC 4 cut(s) 161, 423, 441, 705
DpnII GATC 4 cut(s) 159, 421, 439, 703
DraIII CACNNNGTG 1 cut(s) 693
Eam1104I CTCTTC 3 cut(s) 207, 236, 340
EarI CTCTTC 3 cut(s) 207, 236, 340
EciI GGCGGA 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 219
Eco32I GATATC 1 cut(s) 395
Eco47I GGWCC 1 cut(s) 696
EcoRI GAATTC 1 cut(s) 404
EcoRII CCWGG 2 cut(s) 30, 519
EcoRV GATATC 1 cut(s) 395
EcoT14I CCWWGG 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 3 cut(s) 26, 183, 299
FaiI YATR 8 cut(s) 24, 150, 181, 297, 302, 369, 549, 665
FalI AAGNNNNNCTT 3 cut(s) 264, 296, 729
FatI CATG 3 cut(s) 22, 179, 295
FbaI TGATCA 1 cut(s) 439
Fnu4HI GCNGC 3 cut(s) 435, 480, 668
FokI GGATG 2 cut(s) 116, 489
Fsp4HI GCNGC 3 cut(s) 435, 480, 668
FspBI CTAG 3 cut(s) 398, 584, 642
GluI GCNGC 3 cut(s) 435, 480, 668
HaeIII GGCC 2 cut(s) 218, 519
HapII CCGG 1 cut(s) 566
HgaI GACGC 1 cut(s) 165
Hin1I GRCGYC 1 cut(s) 176
Hin1II CATG 3 cut(s) 26, 183, 299
HinfI GANTC 2 cut(s) 350, 638
HpaII CCGG 1 cut(s) 566
HphI GGTGA 4 cut(s) 239, 314, 428, 731
Hpy166II GTNNAC 3 cut(s) 230, 342, 696
Hpy188I TCNGA 2 cut(s) 487, 703
Hpy188III TCNNGA 1 cut(s) 749
Hpy8I GTNNAC 3 cut(s) 230, 342, 696
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 4 cut(s) 165, 335, 586, 755
HpyCH4III ACNGT 3 cut(s) 192, 310, 358
HpyCH4V TGCA 1 cut(s) 667
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 41, 50, 173, 593, 651
HpyF3I CTNAG 3 cut(s) 486, 588, 700
Hsp92I GRCGYC 1 cut(s) 176
Hsp92II CATG 3 cut(s) 26, 183, 299
Ksp22I TGATCA 1 cut(s) 439
Kzo9I GATC 4 cut(s) 159, 421, 439, 703
LmnI GCTCC 1 cut(s) 124
Lsp1109I GCAGC 3 cut(s) 421, 466, 679
LweI GCATC 1 cut(s) 636
MaeI CTAG 3 cut(s) 398, 584, 642
MalI GATC 4 cut(s) 161, 423, 441, 705
MboI GATC 4 cut(s) 159, 421, 439, 703
MboII GAAGA 8 cut(s) 89, 97, 186, 224, 253, 284, 327, 749
MflI RGATCY 1 cut(s) 703
MluCI AATT 5 cut(s) 110, 130, 288, 404, 552
MmeI TCCRAC 1 cut(s) 81
MroXI GAANNNNTTC 1 cut(s) 744
MseI TTAA 2 cut(s) 450, 729
MspI CCGG 1 cut(s) 566
MspR9I CCNGG 3 cut(s) 32, 521, 566
MvaI CCWGG 2 cut(s) 32, 521
MwoI GCNNNNNNNGC 6 cut(s) 32, 41, 50, 173, 593, 651
NciI CCSGG 1 cut(s) 566
NdeII GATC 4 cut(s) 159, 421, 439, 703
NlaIII CATG 3 cut(s) 26, 183, 299
NmeAIII GCCGAG 2 cut(s) 70, 495
NspI RCATGY 1 cut(s) 26
PaeI GCATGC 1 cut(s) 26
PdmI GAANNNNTTC 1 cut(s) 744
PfeI GAWTC 2 cut(s) 350, 638
PkrI GCNGC 3 cut(s) 436, 481, 669
Psp6I CCWGG 2 cut(s) 30, 519
PspGI CCWGG 2 cut(s) 30, 519
PspPI GGNCC 1 cut(s) 696
PsuI RGATCY 1 cut(s) 703
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
SaqAI TTAA 2 cut(s) 450, 729
SatI GCNGC 3 cut(s) 435, 480, 668
Sau3AI GATC 4 cut(s) 159, 421, 439, 703
Sau96I GGNCC 1 cut(s) 696
ScrFI CCNGG 3 cut(s) 32, 521, 566
SetI ASST 8 cut(s) 282, 327, 403, 436, 589, 672, 701, 721
SfaNI GCATC 1 cut(s) 636
SinI GGWCC 1 cut(s) 696
SphI GCATGC 1 cut(s) 26
Sse9I AATT 5 cut(s) 110, 130, 288, 404, 552
SsiI CCGC 2 cut(s) 184, 467
SspMI CTAG 3 cut(s) 398, 584, 642
StyD4I CCNGG 3 cut(s) 30, 519, 564
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 3 cut(s) 192, 310, 358
TaqI TCGA 2 cut(s) 391, 630
TasI AATT 5 cut(s) 110, 130, 288, 404, 552
TatI WGTACW 1 cut(s) 255
TfiI GAWTC 2 cut(s) 350, 638
Tru1I TTAA 2 cut(s) 450, 729
Tru9I TTAA 2 cut(s) 450, 729
TscAI CASTG 1 cut(s) 349
TseI GCWGC 3 cut(s) 434, 479, 667
TspDTI ATGAA 3 cut(s) 98, 389, 564
TspRI CASTG 1 cut(s) 349
VpaK11BI GGWCC 1 cut(s) 696
XapI RAATTY 4 cut(s) 130, 288, 404, 552
XceI RCATGY 1 cut(s) 26
XcmI CCANNNNNNNNNTGG 1 cut(s) 103
XmnI GAANNNNTTC 1 cut(s) 744
XspI CTAG 3 cut(s) 398, 584, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.