RLG00000000816

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0010696
Physical Location & Seq
Reverse (-)
5916145 .. 5919337
3193 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000816

Sequence Viewer

Length: 816 bp
ATGGAGAAGGATAGGGAGAGTCATGTTTACAGGGCCAGACTTGCTGAGCAAGCCGAAAGATATGATGAAATGATTGAAGTAATGAAAAAAGTTGCTAAGCTGGATGTGGAATTGACCGTGGAAGAGCGAAATTTGTTGTCTGTTGGGTATAAGAATGTGATTGGGGCAAGAAGGGCATCATGGCGGATACTGTCTTCCATTGAACAGAAGGAGGAGGCCAAGGGAAATGAGCAGAATGTGAAGCGAATAAAGGAGTACATGCAAAGGATTGAAGATGAGCTTGCAATTATTTGCAAGGACATGTTATCAGTGATTGATCATCACCTCCTCCCTTCTTCATCAACTGGGGAATCGACTGTATTCTACCATAAGATGAAAGGAGATTACTATCGTTATTTAGCTGAATTCAAAGCAGGTGATGATCGTGCAGAAGCTGCTGATCAGTCGCTTAAAGCCTATGAGGTATGTTTAGCTGCCAGTAGTAGTGCGGCCTCTCAGTTGCCCCCAACTCATCCAATCAGACTGGGCCTTGCTCTGAACTACTCAGTCTTTTACTATGAAATTCTTAAATCCTCTGAGAGGTTGCTTAAATGTCCCCCCCCTGATTATTCTACATTCTACATTCTTCAGTCTATTTCTTTTACTTTAGCATTTGATGATGCCGTTGCTGAGGTTGACAGTCTTGATCAAGAATCCTACAAGGACAGCACCCTCATTATGCAGCTTCTTAGGGATAATCTCACATTGTGGAACTCGGATCTGCCGGAGCGAGGAGGTGAGCACCCTAAGGTTGATCAATCCCAGCCGGAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

272

Amino Acids

31.06

Weight (kDa)

5.16

Isoelectric Point (pI)

52.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 12 - 251 5.1e-90 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010696)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22300 AT1G22300 AT1G22300 AT1G34760 AT1G34760
fragaria_vesca FvH4_5g37150
malus_domestica MD08G1184000.v1.1 MD15G1370500.v1.1
prunus_persica Prupe.1G518200_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0238741
rosa_laevigata RLG00000000816
rosa_multiflora Rmu_sc0010914.1_g000010
rosa_roxburghii Rroxscaffold_3G00221590
rosa_rugosa Rorug07G0323200.1 Rorug07G0323300.1
rosa_samantha Rh7AG477600 Rh7BG448900 Rh7CG495600 Rh7DG462600
rosa_wichuraiana Rw7G039860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 404
Acc36I ACCTGC 1 cut(s) 404
AciI CCGC 2 cut(s) 184, 488
AclWI GGATC 1 cut(s) 765
AcsI RAATTY 3 cut(s) 130, 404, 561
AcuI CTGAAG 1 cut(s) 611
AdeI CACNNNGTG 1 cut(s) 747
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 2 cut(s) 579, 770
AflIII ACRYGT 1 cut(s) 300
AgsI TTSAA 4 cut(s) 77, 203, 272, 409
AluBI AGCT 6 cut(s) 100, 280, 401, 434, 473, 724
AluI AGCT 6 cut(s) 100, 280, 401, 434, 473, 724
Alw21I GWGCWC 1 cut(s) 783
AlwI GGATC 1 cut(s) 765
AlwNI CAGNNNCTG 1 cut(s) 434
AoxI GGCC 4 cut(s) 33, 216, 489, 526
ApeKI GCWGC 3 cut(s) 434, 473, 721
ApoI RAATTY 3 cut(s) 130, 404, 561
AspS9I GGNCC 2 cut(s) 33, 526
AsuHPI GGTGA 3 cut(s) 314, 428, 788
AxyI CCTNAGG 1 cut(s) 786
BbsI GAAGAC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 783
BbvCI CCTCAGC 1 cut(s) 669
BbvI GCAGC 3 cut(s) 421, 460, 733
BceAI ACGGC 1 cut(s) 647
BciVI GTATCC 1 cut(s) 180
BclI TGATCA 4 cut(s) 316, 439, 685, 793
BfuAI ACCTGC 1 cut(s) 404
BfuI GTATCC 1 cut(s) 180
BisI GCNGC 4 cut(s) 435, 474, 489, 722
BlpI GCTNAGC 2 cut(s) 45, 96
BlsI GCNGC 4 cut(s) 436, 475, 490, 723
BmgT120I GGNCC 2 cut(s) 33, 526
BmrI ACTGGG 2 cut(s) 354, 533
BmsI GCATC 2 cut(s) 185, 649
BmuI ACTGGG 2 cut(s) 354, 533
BpiI GAAGAC 1 cut(s) 186
Bpu10I CCTNAGC 1 cut(s) 669
Bpu1102I GCTNAGC 2 cut(s) 45, 96
BsaBI GATNNNNATC 1 cut(s) 387
BsaJI CCNNGG 2 cut(s) 117, 219
Bsc4I CCNNNNNNNGG 2 cut(s) 579, 770
Bse1I ACTGG 3 cut(s) 349, 477, 528
Bse21I CCTNAGG 1 cut(s) 786
Bse8I GATNNNNATC 1 cut(s) 387
BseDI CCNNGG 2 cut(s) 117, 219
BseGI GGATG 2 cut(s) 109, 511
BseJI GATNNNNATC 1 cut(s) 387
BseLI CCNNNNNNNGG 2 cut(s) 579, 770
BseMII CTCAG 5 cut(s) 36, 509, 558, 567, 660
BseNI ACTGG 3 cut(s) 349, 477, 528
BseRI GAGGAG 3 cut(s) 227, 317, 786
BseXI GCAGC 3 cut(s) 421, 460, 733
BseYI CCCAGC 1 cut(s) 801
BsgI GTGCAG 1 cut(s) 447
BshFI GGCC 4 cut(s) 35, 218, 491, 528
BsiHKAI GWGCWC 1 cut(s) 783
BsiSI CCGG 2 cut(s) 764, 806
BslFI GGGAC 1 cut(s) 579
BslI CCNNNNNNNGG 2 cut(s) 579, 770
BsmFI GGGAC 1 cut(s) 579
BsnI GGCC 4 cut(s) 35, 218, 491, 528
Bsp1286I GDGCHC 1 cut(s) 783
Bsp143I GATC 6 cut(s) 316, 421, 439, 685, 757, 793
Bsp1720I GCTNAGC 2 cut(s) 45, 96
BspACI CCGC 2 cut(s) 184, 488
BspANI GGCC 4 cut(s) 35, 218, 491, 528
BspCNI CTCAG 5 cut(s) 37, 508, 557, 568, 661
BspMI ACCTGC 1 cut(s) 404
BspPI GGATC 1 cut(s) 765
BspQI GCTCTTC 1 cut(s) 117
BsrI ACTGG 3 cut(s) 349, 477, 528
BssECI CCNNGG 2 cut(s) 117, 219
BssMI GATC 6 cut(s) 316, 421, 439, 685, 757, 793
BssT1I CCWWGG 1 cut(s) 219
Bst4CI ACNGT 4 cut(s) 118, 192, 358, 680
Bst6I CTCTTC 1 cut(s) 117
BstAPI GCANNNNNTGC 1 cut(s) 434
BstC8I GCNNGC 2 cut(s) 51, 282
BstDEI CTNAG 8 cut(s) 45, 96, 495, 544, 576, 669, 728, 786
BstDSI CCRYGG 1 cut(s) 117
BstENI CCTNNNNNAGG 1 cut(s) 577
BstF5I GGATG 2 cut(s) 109, 511
BstKTI GATC 6 cut(s) 319, 424, 442, 688, 760, 796
BstMBI GATC 6 cut(s) 316, 421, 439, 685, 757, 793
BstMWI GCNNNNNNNGC 4 cut(s) 41, 50, 173, 434
BstNSI RCATGY 2 cut(s) 262, 304
BstV1I GCAGC 3 cut(s) 421, 460, 733
BstV2I GAAGAC 1 cut(s) 186
BstX2I RGATCY 1 cut(s) 757
BstYI RGATCY 1 cut(s) 757
Bsu36I CCTNAGG 1 cut(s) 786
BsuI GTATCC 1 cut(s) 180
BsuRI GGCC 4 cut(s) 35, 218, 491, 528
BtgI CCRYGG 1 cut(s) 117
BtsCI GGATG 2 cut(s) 109, 511
BtsIMutI CAGTG 1 cut(s) 315
BveI ACCTGC 1 cut(s) 404
Cac8I GCNNGC 2 cut(s) 51, 282
CaiI CAGNNNCTG 1 cut(s) 434
Cfr13I GGNCC 2 cut(s) 33, 526
Csp6I GTAC 1 cut(s) 256
CviAII CATG 4 cut(s) 23, 180, 259, 301
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 8 cut(s) 45, 96, 495, 544, 576, 669, 728, 786
DpnI GATC 6 cut(s) 318, 423, 441, 687, 759, 795
DpnII GATC 6 cut(s) 316, 421, 439, 685, 757, 793
DraIII CACNNNGTG 1 cut(s) 747
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
EciI GGCGGA 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 219
Eco57I CTGAAG 1 cut(s) 611
Eco81I CCTNAGG 1 cut(s) 786
EcoNI CCTNNNNNAGG 1 cut(s) 577
EcoRI GAATTC 1 cut(s) 404
EcoT14I CCWWGG 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 4 cut(s) 26, 183, 262, 304
FalI AAGNNNNNCTT 2 cut(s) 264, 296
FaqI GGGAC 1 cut(s) 579
FatI CATG 4 cut(s) 22, 179, 258, 300
FbaI TGATCA 4 cut(s) 316, 439, 685, 793
Fnu4HI GCNGC 4 cut(s) 435, 474, 489, 722
FokI GGATG 2 cut(s) 116, 498
Fsp4HI GCNGC 4 cut(s) 435, 474, 489, 722
GluI GCNGC 4 cut(s) 435, 474, 489, 722
GsaI CCCAGC 1 cut(s) 805
HaeIII GGCC 4 cut(s) 35, 218, 491, 528
HapII CCGG 2 cut(s) 764, 806
Hin1II CATG 4 cut(s) 26, 183, 262, 304
HincII GTYRAC 1 cut(s) 676
HindII GTYRAC 1 cut(s) 676
HinfI GANTC 3 cut(s) 19, 350, 692
HpaII CCGG 2 cut(s) 764, 806
HphI GGTGA 3 cut(s) 314, 428, 788
Hpy166II GTNNAC 2 cut(s) 28, 676
Hpy188I TCNGA 4 cut(s) 521, 537, 577, 757
Hpy188III TCNNGA 2 cut(s) 683, 689
Hpy8I GTNNAC 2 cut(s) 28, 676
HpyAV CCTTC 3 cut(s) 165, 202, 342
HpyCH4III ACNGT 4 cut(s) 118, 192, 358, 680
HpyCH4V TGCA 5 cut(s) 262, 284, 294, 428, 721
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 50, 173, 434
HpyF3I CTNAG 8 cut(s) 45, 96, 495, 544, 576, 669, 728, 786
Hsp92II CATG 4 cut(s) 26, 183, 262, 304
Ksp22I TGATCA 4 cut(s) 316, 439, 685, 793
Kzo9I GATC 6 cut(s) 316, 421, 439, 685, 757, 793
LguI GCTCTTC 1 cut(s) 117
LmnI GCTCC 1 cut(s) 766
LpnPI CCDG 9 cut(s) 16, 49, 86, 330, 399, 490, 509, 615, 777
Lsp1109I GCAGC 3 cut(s) 421, 460, 733
LweI GCATC 2 cut(s) 185, 649
MalI GATC 6 cut(s) 318, 423, 441, 687, 759, 795
MboI GATC 6 cut(s) 316, 421, 439, 685, 757, 793
MboII GAAGA 5 cut(s) 134, 186, 284, 327, 617
MflI RGATCY 1 cut(s) 757
MhlI GDGCHC 1 cut(s) 783
MluCI AATT 5 cut(s) 110, 130, 285, 404, 561
MlyI GAGTC 1 cut(s) 28
MseI TTAA 3 cut(s) 450, 567, 588
MspI CCGG 2 cut(s) 764, 806
MwoI GCNNNNNNNGC 4 cut(s) 41, 50, 173, 434
NdeII GATC 6 cut(s) 316, 421, 439, 685, 757, 793
NlaIII CATG 4 cut(s) 26, 183, 262, 304
NspI RCATGY 2 cut(s) 262, 304
PaqCI CACCTGC 1 cut(s) 404
PciI ACATGT 1 cut(s) 300
PciSI GCTCTTC 1 cut(s) 117
PfeI GAWTC 2 cut(s) 350, 692
PkrI GCNGC 4 cut(s) 436, 475, 490, 723
PleI GAGTC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 27
PscI ACATGT 1 cut(s) 300
PspFI CCCAGC 1 cut(s) 801
PspPI GGNCC 2 cut(s) 33, 526
PstNI CAGNNNCTG 1 cut(s) 434
PsuI RGATCY 1 cut(s) 757
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
SapI GCTCTTC 1 cut(s) 117
SaqAI TTAA 3 cut(s) 450, 567, 588
SatI GCNGC 4 cut(s) 435, 474, 489, 722
Sau3AI GATC 6 cut(s) 316, 421, 439, 685, 757, 793
Sau96I GGNCC 2 cut(s) 33, 526
SchI GAGTC 1 cut(s) 28
SduI GDGCHC 1 cut(s) 783
SfaNI GCATC 2 cut(s) 185, 649
Sse9I AATT 5 cut(s) 110, 130, 285, 404, 561
SsiI CCGC 2 cut(s) 184, 488
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 4 cut(s) 118, 192, 358, 680
TaqI TCGA 1 cut(s) 353
TasI AATT 5 cut(s) 110, 130, 285, 404, 561
TatI WGTACW 1 cut(s) 255
TauI GCSGC 1 cut(s) 491
TfiI GAWTC 2 cut(s) 350, 692
Tru1I TTAA 3 cut(s) 450, 567, 588
Tru9I TTAA 3 cut(s) 450, 567, 588
TscAI CASTG 1 cut(s) 315
TseI GCWGC 3 cut(s) 434, 473, 721
TspDTI ATGAA 5 cut(s) 81, 98, 327, 389, 573
TspRI CASTG 1 cut(s) 315
XagI CCTNNNNNAGG 1 cut(s) 577
XapI RAATTY 3 cut(s) 130, 404, 561
XceI RCATGY 2 cut(s) 262, 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.