MD15G1370500.v1.1

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010696
Physical Location & Seq
Reverse (-)
45033182 .. 45036653
3472 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1370500.v1.1.491

Sequence Viewer

Length: 768 bp
ATGGAGATAGAGAGAGAGAAGCATGCTTACCAGGCAAAGCTTGCTGAGCAAGCCGAAAGATACGATGAGATGGTTGAAGAAATGAAGAAAGTTGCCGAGTTGGATGTGGAACTGAGCGTGGAGGAGAGACATTTGGTGTCGGTTGGGTATAAGAATGTGATTGGGGCAAGACGGGCGTCATGGCGGATACTGTCTTCAATTGAACAGAAGGAGGAGGCAAAGGGAAGTGAACAATATGCGAAGAGGATAAAGGAGTACAGGAAGAGGGTTGAAGACGAGCTTGCAAAAATTTGCCATGATATACTGACAGTTGTTGATAATCACCTCCTCCCATCAGCGTCCACAGGGGAATCCACTGTTTTTTACCAGAAGATGAAAGGAGATTACTATCGATATCTAGCTGAATTCAAAGCTGGTGATGATCGTAAAGAAGCTGCTGATCAGTCGCTCAAAGCCTATGTGAGTGCCATTGATACTGCTGCCTCTGAGTTTCCCCCAACACACCCAATCAGGCTTGGCCTGGCTCTAAACTTTTCTGTTTTCTACTACGAAATTCAAAACTCCCCTGAGAGGGCGTGTCATGTAGCTAAGAAGGCATTCGATGAGGCTATTGCCGAACTTGACAGCCTCGACGAAGAATCCTACAAAGACAGTTCCCTCATTATGCAGCTTCTCAAAGATAATCTCACATTGTGGACCTCGGATCTGCCAGAGGGAGGTGAGCAATTTAAAGTTGAAGAACCCCATCCAGAGGTAAGGGAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

256

Amino Acids

29.3

Weight (kDa)

4.98

Isoelectric Point (pI)

48.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 12 - 233 3e-104 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010696)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22300 AT1G22300 AT1G22300 AT1G34760 AT1G34760
fragaria_vesca FvH4_5g37150
malus_domestica MD08G1184000.v1.1 MD15G1370500.v1.1
prunus_persica Prupe.1G518200_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0238741
rosa_laevigata RLG00000000816
rosa_multiflora Rmu_sc0010914.1_g000010
rosa_roxburghii Rroxscaffold_3G00221590
rosa_rugosa Rorug07G0323200.1 Rorug07G0323300.1
rosa_samantha Rh7AG477600 Rh7BG448900 Rh7CG495600 Rh7DG462600
rosa_wichuraiana Rw7G039860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 184
AclWI GGATC 1 cut(s) 711
AcsI RAATTY 3 cut(s) 288, 404, 552
AcyI GRCGYC 1 cut(s) 176
AdeI CACNNNGTG 1 cut(s) 693
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 4 cut(s) 570, 571, 716, 751
AgsI TTSAA 7 cut(s) 77, 198, 203, 272, 409, 557, 737
AjnI CCWGG 2 cut(s) 30, 519
AluBI AGCT 7 cut(s) 40, 280, 401, 413, 434, 587, 670
AluI AGCT 7 cut(s) 40, 280, 401, 413, 434, 587, 670
Alw26I GTCTC 1 cut(s) 121
AlwI GGATC 1 cut(s) 711
AoxI GGCC 1 cut(s) 517
ApeKI GCWGC 3 cut(s) 434, 479, 667
ApoI RAATTY 3 cut(s) 288, 404, 552
Asp700I GAANNNNTTC 1 cut(s) 596
AspS9I GGNCC 1 cut(s) 696
AsuHPI GGTGA 3 cut(s) 314, 428, 731
AvaII GGWCC 1 cut(s) 696
BarI GAAGNNNNNNTAC 2 cut(s) 11, 43
BbsI GAAGAC 2 cut(s) 186, 279
BbvI GCAGC 3 cut(s) 421, 466, 679
BccI CCATC 3 cut(s) 64, 340, 753
BciT130I CCWGG 2 cut(s) 32, 521
BciVI GTATCC 1 cut(s) 180
BclI TGATCA 1 cut(s) 439
BcoDI GTCTC 1 cut(s) 121
BfaI CTAG 1 cut(s) 398
BfuI GTATCC 1 cut(s) 180
BisI GCNGC 3 cut(s) 435, 480, 668
BlpI GCTNAGC 1 cut(s) 45
BlsI GCNGC 3 cut(s) 436, 481, 669
Bme1390I CCNGG 2 cut(s) 32, 521
Bme18I GGWCC 1 cut(s) 696
BmgT120I GGNCC 1 cut(s) 696
BmrFI CCNGG 2 cut(s) 32, 521
BoxI GACNNNNGTC 1 cut(s) 175
BpiI GAAGAC 2 cut(s) 186, 279
Bpu1102I GCTNAGC 1 cut(s) 45
Bsa29I ATCGAT 1 cut(s) 391
BsaBI GATNNNNATC 1 cut(s) 387
BsaHI GRCGYC 1 cut(s) 176
BsaJI CCNNGG 1 cut(s) 699
Bsc4I CCNNNNNNNGG 4 cut(s) 570, 571, 716, 751
Bse8I GATNNNNATC 1 cut(s) 387
BseBI CCWGG 2 cut(s) 32, 521
BseCI ATCGAT 1 cut(s) 391
BseDI CCNNGG 1 cut(s) 699
BseGI GGATG 2 cut(s) 109, 745
BseJI GATNNNNATC 1 cut(s) 387
BseLI CCNNNNNNNGG 4 cut(s) 570, 571, 716, 751
BseMII CTCAG 4 cut(s) 36, 104, 477, 558
BseRI GAGGAG 3 cut(s) 137, 227, 317
BseXI GCAGC 3 cut(s) 421, 466, 679
BshFI GGCC 1 cut(s) 519
BshVI ATCGAT 1 cut(s) 391
BslI CCNNNNNNNGG 4 cut(s) 570, 571, 716, 751
BsmAI GTCTC 1 cut(s) 121
BsmI GAATGC 1 cut(s) 596
BsnI GGCC 1 cut(s) 519
Bsp143I GATC 3 cut(s) 421, 439, 703
Bsp1720I GCTNAGC 1 cut(s) 45
BspACI CCGC 1 cut(s) 184
BspANI GGCC 1 cut(s) 519
BspCNI CTCAG 4 cut(s) 37, 105, 478, 559
BspDI ATCGAT 1 cut(s) 391
BspPI GGATC 1 cut(s) 711
BssECI CCNNGG 1 cut(s) 699
BssMI GATC 3 cut(s) 421, 439, 703
BssNI GRCGYC 1 cut(s) 176
Bst2UI CCWGG 2 cut(s) 32, 521
Bst4CI ACNGT 4 cut(s) 192, 310, 358, 653
Bst6I CTCTTC 2 cut(s) 236, 257
BstACI GRCGYC 1 cut(s) 176
BstAPI GCANNNNNTGC 1 cut(s) 41
BstC8I GCNNGC 4 cut(s) 24, 42, 51, 282
BstDEI CTNAG 5 cut(s) 45, 113, 486, 567, 588
BstF5I GGATG 2 cut(s) 109, 745
BstKTI GATC 3 cut(s) 424, 442, 706
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 3 cut(s) 421, 439, 703
BstMWI GCNNNNNNNGC 6 cut(s) 32, 41, 46, 50, 173, 593
BstNI CCWGG 2 cut(s) 32, 521
BstNSI RCATGY 1 cut(s) 26
BstPAI GACNNNNGTC 1 cut(s) 175
BstSCI CCNGG 2 cut(s) 30, 519
BstV1I GCAGC 3 cut(s) 421, 466, 679
BstV2I GAAGAC 2 cut(s) 186, 279
BstX2I RGATCY 1 cut(s) 703
BstYI RGATCY 1 cut(s) 703
Bsu15I ATCGAT 1 cut(s) 391
BsuI GTATCC 1 cut(s) 180
BsuRI GGCC 1 cut(s) 519
BsuTUI ATCGAT 1 cut(s) 391
BtsCI GGATG 2 cut(s) 109, 745
BtsIMutI CAGTG 1 cut(s) 354
Cac8I GCNNGC 4 cut(s) 24, 42, 51, 282
Cfr13I GGNCC 1 cut(s) 696
ClaI ATCGAT 1 cut(s) 391
CseI GACGC 2 cut(s) 165, 327
Csp6I GTAC 1 cut(s) 256
CviAII CATG 4 cut(s) 23, 180, 296, 581
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 5 cut(s) 45, 113, 486, 567, 588
DpnI GATC 3 cut(s) 423, 441, 705
DpnII GATC 3 cut(s) 421, 439, 703
DraI TTTAAA 1 cut(s) 730
DraIII CACNNNGTG 1 cut(s) 693
Eam1104I CTCTTC 2 cut(s) 236, 257
EarI CTCTTC 2 cut(s) 236, 257
EciI GGCGGA 1 cut(s) 199
Eco32I GATATC 1 cut(s) 395
Eco47I GGWCC 1 cut(s) 696
EcoRI GAATTC 1 cut(s) 404
EcoRII CCWGG 2 cut(s) 30, 519
EcoRV GATATC 1 cut(s) 395
FaeI CATG 4 cut(s) 26, 183, 299, 584
FaiI YATR 9 cut(s) 24, 150, 181, 237, 297, 302, 459, 582, 665
FalI AAGNNNNNCTT 2 cut(s) 264, 296
FatI CATG 4 cut(s) 22, 179, 295, 580
FbaI TGATCA 1 cut(s) 439
Fnu4HI GCNGC 3 cut(s) 435, 480, 668
FokI GGATG 2 cut(s) 116, 732
Fsp4HI GCNGC 3 cut(s) 435, 480, 668
FspBI CTAG 1 cut(s) 398
GluI GCNGC 3 cut(s) 435, 480, 668
HaeIII GGCC 1 cut(s) 519
HgaI GACGC 2 cut(s) 165, 327
Hin1I GRCGYC 1 cut(s) 176
Hin1II CATG 4 cut(s) 26, 183, 299, 584
HindIII AAGCTT 1 cut(s) 38
HinfI GANTC 2 cut(s) 350, 638
HphI GGTGA 3 cut(s) 314, 428, 731
Hpy166II GTNNAC 3 cut(s) 230, 342, 696
Hpy188I TCNGA 2 cut(s) 487, 703
Hpy188III TCNNGA 1 cut(s) 749
Hpy8I GTNNAC 3 cut(s) 230, 342, 696
Hpy99I CGWCG 1 cut(s) 635
HpyAV CCTTC 2 cut(s) 202, 586
HpyCH4III ACNGT 4 cut(s) 192, 310, 358, 653
HpyCH4V TGCA 2 cut(s) 284, 667
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 41, 46, 50, 173, 593
HpyF3I CTNAG 5 cut(s) 45, 113, 486, 567, 588
Hsp92I GRCGYC 1 cut(s) 176
Hsp92II CATG 4 cut(s) 26, 183, 299, 584
Ksp22I TGATCA 1 cut(s) 439
Kzo9I GATC 3 cut(s) 421, 439, 703
Lsp1109I GCAGC 3 cut(s) 421, 466, 679
MaeI CTAG 1 cut(s) 398
MalI GATC 3 cut(s) 423, 441, 705
MboI GATC 3 cut(s) 421, 439, 703
MboII GAAGA 9 cut(s) 89, 97, 186, 253, 274, 284, 382, 647, 749
MfeI CAATTG 1 cut(s) 198
MflI RGATCY 1 cut(s) 703
MluCI AATT 5 cut(s) 198, 288, 404, 552, 725
MmeI TCCRAC 1 cut(s) 81
MroXI GAANNNNTTC 1 cut(s) 596
MseI TTAA 1 cut(s) 729
MspR9I CCNGG 2 cut(s) 32, 521
MunI CAATTG 1 cut(s) 198
Mva1269I GAATGC 1 cut(s) 596
MvaI CCWGG 2 cut(s) 32, 521
MwoI GCNNNNNNNGC 6 cut(s) 32, 41, 46, 50, 173, 593
NdeII GATC 3 cut(s) 421, 439, 703
NlaIII CATG 4 cut(s) 26, 183, 299, 584
NmeAIII GCCGAG 1 cut(s) 121
NspI RCATGY 1 cut(s) 26
PaeI GCATGC 1 cut(s) 26
PctI GAATGC 1 cut(s) 596
PdmI GAANNNNTTC 1 cut(s) 596
PfeI GAWTC 2 cut(s) 350, 638
PkrI GCNGC 3 cut(s) 436, 481, 669
PshAI GACNNNNGTC 1 cut(s) 175
Psp6I CCWGG 2 cut(s) 30, 519
PspGI CCWGG 2 cut(s) 30, 519
PspPI GGNCC 1 cut(s) 696
PsuI RGATCY 1 cut(s) 703
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
SaqAI TTAA 1 cut(s) 729
SatI GCNGC 3 cut(s) 435, 480, 668
Sau3AI GATC 3 cut(s) 421, 439, 703
Sau96I GGNCC 1 cut(s) 696
ScrFI CCNGG 2 cut(s) 32, 521
SinI GGWCC 1 cut(s) 696
SphI GCATGC 1 cut(s) 26
Sse9I AATT 5 cut(s) 198, 288, 404, 552, 725
SsiI CCGC 1 cut(s) 184
SspMI CTAG 1 cut(s) 398
StyD4I CCNGG 2 cut(s) 30, 519
TaaI ACNGT 4 cut(s) 192, 310, 358, 653
TaqI TCGA 3 cut(s) 391, 600, 630
TasI AATT 5 cut(s) 198, 288, 404, 552, 725
TatI WGTACW 1 cut(s) 255
TfiI GAWTC 2 cut(s) 350, 638
Tru1I TTAA 1 cut(s) 729
Tru9I TTAA 1 cut(s) 729
TscAI CASTG 1 cut(s) 361
TseI GCWGC 3 cut(s) 434, 479, 667
TspDTI ATGAA 2 cut(s) 98, 389
TspRI CASTG 1 cut(s) 361
VpaK11BI GGWCC 1 cut(s) 696
XapI RAATTY 3 cut(s) 288, 404, 552
XceI RCATGY 1 cut(s) 26
XmnI GAANNNNTTC 1 cut(s) 596
XspI CTAG 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.