Rh7DG462600

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0010696
Physical Location & Seq
Forward (+)
65869060 .. 65872862
3803 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG462600.1

Sequence Viewer

Length: 774 bp
ATGGAGAAGGAGAAGGATAGGGAGAGTCATGTTTACCGGGCCAAACTTGCTGAGCAAGCCGAAAGATATGATGAAATGATTGAAGTAATGAAAAAAGTTGCTAAGCTGGATGTGGAATTGACCGTGGATGAGCGAAATTTGTTGTCTGTTGGGTATAAGAATGTGATTGGGGCAAGAAGGGCATCATGGCGGATACTGTCTTCCATTGAACAGAAGGAGGAGGCCAAGGGAAATGAGCAGAATGTGAAGCGAATAAAGGAGTACATGCAAAGGGTTGAAGATGAGCTTGCAACTATTTGCAAGGACATGTTATCAGTGATTGATTATCACCTCCTCCCTTCTTCATCAACCGGGGAATCGACTGTATTCTACCATAAGATGAAAGGAGATTACTATCGTTATTTAGCTGAATTCAAAGCAGGTGATGATCGTGCAGAAGCTGCTGATCAGTCGCTTAAAGCCTATGAGGCTGCCAGTAGTAGTGCGGCCTCTCAGTTGCCCCCAACTCATCCAATCAGACTGGGCCTTGCTCTGAACTACTCAGTCTTTTACTATGAAATTCTTAAATCCTCTGAGAGGGCATGCGATCTTGCTAAGCAGGCATTTGATGATGCCGTTGCTGAGGTTGACAGTCTTGATCAAGAATCCTACAAGGACAGCACCCTCATTATGCAGCTTCTTAGGGATAATCTCACATTGTGGAACTCGGATCTGTCGGAGGGAGGAGGTAATCTTATGAGTTGTTTCATTTCCTTCCACCTTATTTTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

257

Amino Acids

29.33

Weight (kDa)

5.14

Isoelectric Point (pI)

47.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 14 - 235 4.3e-104 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010696)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22300 AT1G22300 AT1G22300 AT1G34760 AT1G34760
fragaria_vesca FvH4_5g37150
malus_domestica MD08G1184000.v1.1 MD15G1370500.v1.1
prunus_persica Prupe.1G518200_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0238741
rosa_laevigata RLG00000000816
rosa_multiflora Rmu_sc0010914.1_g000010
rosa_roxburghii Rroxscaffold_3G00221590
rosa_rugosa Rorug07G0323200.1 Rorug07G0323300.1
rosa_samantha Rh7AG477600 Rh7BG448900 Rh7CG495600 Rh7DG462600
rosa_wichuraiana Rw7G039860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 410
Acc36I ACCTGC 1 cut(s) 410
AciI CCGC 2 cut(s) 190, 485
AclWI GGATC 1 cut(s) 717
AcsI RAATTY 3 cut(s) 136, 410, 558
AdeI CACNNNGTG 1 cut(s) 699
AfaI GTAC 1 cut(s) 263
AfiI CCNNNNNNNGG 1 cut(s) 576
AflIII ACRYGT 1 cut(s) 306
AgsI TTSAA 5 cut(s) 83, 209, 278, 415, 769
AluBI AGCT 5 cut(s) 106, 286, 407, 440, 676
AluI AGCT 5 cut(s) 106, 286, 407, 440, 676
AlwI GGATC 1 cut(s) 717
AlwNI CAGNNNCTG 1 cut(s) 440
AoxI GGCC 4 cut(s) 39, 222, 486, 523
ApeKI GCWGC 3 cut(s) 440, 470, 673
ApoI RAATTY 3 cut(s) 136, 410, 558
AspS9I GGNCC 2 cut(s) 39, 523
AsuC2I CCSGG 2 cut(s) 38, 352
AsuHPI GGTGA 2 cut(s) 320, 434
BbsI GAAGAC 1 cut(s) 192
BbvCI CCTCAGC 1 cut(s) 621
BbvI GCAGC 3 cut(s) 427, 457, 685
BceAI ACGGC 1 cut(s) 599
BciVI GTATCC 1 cut(s) 186
BclI TGATCA 2 cut(s) 445, 637
BcnI CCSGG 2 cut(s) 38, 352
BfuAI ACCTGC 1 cut(s) 410
BfuI GTATCC 1 cut(s) 186
BglI GCCNNNNNGGC 1 cut(s) 467
BisI GCNGC 4 cut(s) 441, 471, 486, 674
BlpI GCTNAGC 3 cut(s) 51, 102, 594
BlsI GCNGC 4 cut(s) 442, 472, 487, 675
Bme1390I CCNGG 2 cut(s) 38, 352
BmgT120I GGNCC 2 cut(s) 39, 523
BmrFI CCNGG 2 cut(s) 38, 352
BmrI ACTGGG 1 cut(s) 530
BmsI GCATC 2 cut(s) 191, 601
BmuI ACTGGG 1 cut(s) 530
BpiI GAAGAC 1 cut(s) 192
Bpu10I CCTNAGC 1 cut(s) 621
Bpu1102I GCTNAGC 3 cut(s) 51, 102, 594
BpuMI CCSGG 2 cut(s) 38, 352
BsaBI GATNNNNATC 1 cut(s) 393
BsaJI CCNNGG 3 cut(s) 123, 225, 351
Bsc4I CCNNNNNNNGG 1 cut(s) 576
Bse1I ACTGG 2 cut(s) 474, 525
Bse8I GATNNNNATC 1 cut(s) 393
BseDI CCNNGG 3 cut(s) 123, 225, 351
BseGI GGATG 3 cut(s) 115, 133, 508
BseJI GATNNNNATC 1 cut(s) 393
BseLI CCNNNNNNNGG 1 cut(s) 576
BseMII CTCAG 5 cut(s) 42, 506, 555, 564, 612
BseNI ACTGG 2 cut(s) 474, 525
BseRI GAGGAG 3 cut(s) 233, 323, 738
BseXI GCAGC 3 cut(s) 427, 457, 685
BsgI GTGCAG 1 cut(s) 453
BshFI GGCC 4 cut(s) 41, 224, 488, 525
BsiSI CCGG 2 cut(s) 37, 351
BslI CCNNNNNNNGG 1 cut(s) 576
BsnI GGCC 4 cut(s) 41, 224, 488, 525
Bsp143I GATC 5 cut(s) 427, 445, 586, 637, 709
Bsp1720I GCTNAGC 3 cut(s) 51, 102, 594
BspACI CCGC 2 cut(s) 190, 485
BspANI GGCC 4 cut(s) 41, 224, 488, 525
BspCNI CTCAG 5 cut(s) 43, 505, 554, 565, 613
BspMI ACCTGC 1 cut(s) 410
BspPI GGATC 1 cut(s) 717
BsrI ACTGG 2 cut(s) 474, 525
BssECI CCNNGG 3 cut(s) 123, 225, 351
BssMI GATC 5 cut(s) 427, 445, 586, 637, 709
BssT1I CCWWGG 1 cut(s) 225
Bst4CI ACNGT 4 cut(s) 124, 198, 364, 632
BstAPI GCANNNNNTGC 1 cut(s) 440
BstC8I GCNNGC 4 cut(s) 57, 288, 583, 600
BstDEI CTNAG 8 cut(s) 51, 102, 492, 541, 573, 594, 621, 680
BstDSI CCRYGG 1 cut(s) 123
BstENI CCTNNNNNAGG 1 cut(s) 574
BstF5I GGATG 3 cut(s) 115, 133, 508
BstKTI GATC 5 cut(s) 430, 448, 589, 640, 712
BstMBI GATC 5 cut(s) 427, 445, 586, 637, 709
BstMWI GCNNNNNNNGC 6 cut(s) 47, 56, 179, 440, 467, 599
BstNSI RCATGY 3 cut(s) 268, 310, 585
BstSCI CCNGG 2 cut(s) 36, 350
BstV1I GCAGC 3 cut(s) 427, 457, 685
BstV2I GAAGAC 1 cut(s) 192
BstX2I RGATCY 1 cut(s) 709
BstYI RGATCY 1 cut(s) 709
BsuI GTATCC 1 cut(s) 186
BsuRI GGCC 4 cut(s) 41, 224, 488, 525
BtgI CCRYGG 1 cut(s) 123
BtsCI GGATG 3 cut(s) 115, 133, 508
BtsIMutI CAGTG 1 cut(s) 321
BveI ACCTGC 1 cut(s) 410
Cac8I GCNNGC 4 cut(s) 57, 288, 583, 600
CaiI CAGNNNCTG 1 cut(s) 440
Cfr13I GGNCC 2 cut(s) 39, 523
Csp6I GTAC 1 cut(s) 262
CviAII CATG 5 cut(s) 29, 186, 265, 307, 582
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 8 cut(s) 51, 102, 492, 541, 573, 594, 621, 680
DpnI GATC 5 cut(s) 429, 447, 588, 639, 711
DpnII GATC 5 cut(s) 427, 445, 586, 637, 709
DraIII CACNNNGTG 1 cut(s) 699
EciI GGCGGA 1 cut(s) 205
Eco130I CCWWGG 1 cut(s) 225
EcoNI CCTNNNNNAGG 1 cut(s) 574
EcoRI GAATTC 1 cut(s) 410
EcoT14I CCWWGG 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 225
FaeI CATG 5 cut(s) 32, 189, 268, 310, 585
FalI AAGNNNNNCTT 2 cut(s) 270, 302
FatI CATG 5 cut(s) 28, 185, 264, 306, 581
FbaI TGATCA 2 cut(s) 445, 637
Fnu4HI GCNGC 4 cut(s) 441, 471, 486, 674
FokI GGATG 3 cut(s) 122, 140, 495
Fsp4HI GCNGC 4 cut(s) 441, 471, 486, 674
GluI GCNGC 4 cut(s) 441, 471, 486, 674
HaeIII GGCC 4 cut(s) 41, 224, 488, 525
HapII CCGG 2 cut(s) 37, 351
Hin1II CATG 5 cut(s) 32, 189, 268, 310, 585
HincII GTYRAC 1 cut(s) 628
HindII GTYRAC 1 cut(s) 628
HinfI GANTC 3 cut(s) 25, 356, 644
HpaII CCGG 2 cut(s) 37, 351
HphI GGTGA 2 cut(s) 320, 434
Hpy166II GTNNAC 2 cut(s) 34, 628
Hpy188I TCNGA 5 cut(s) 518, 534, 574, 709, 718
Hpy188III TCNNGA 2 cut(s) 635, 641
Hpy8I GTNNAC 2 cut(s) 34, 628
HpyAV CCTTC 5 cut(s) 7, 171, 208, 348, 763
HpyCH4III ACNGT 4 cut(s) 124, 198, 364, 632
HpyCH4V TGCA 5 cut(s) 268, 290, 300, 434, 673
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 56, 179, 440, 467, 599
HpyF3I CTNAG 8 cut(s) 51, 102, 492, 541, 573, 594, 621, 680
Hsp92II CATG 5 cut(s) 32, 189, 268, 310, 585
Ksp22I TGATCA 2 cut(s) 445, 637
Kzo9I GATC 5 cut(s) 427, 445, 586, 637, 709
LpnPI CCDG 7 cut(s) 50, 92, 364, 405, 487, 506, 584
Lsp1109I GCAGC 3 cut(s) 427, 457, 685
LweI GCATC 2 cut(s) 191, 601
MalI GATC 5 cut(s) 429, 447, 588, 639, 711
MboI GATC 5 cut(s) 427, 445, 586, 637, 709
MboII GAAGA 3 cut(s) 192, 290, 333
MflI RGATCY 1 cut(s) 709
MluCI AATT 4 cut(s) 116, 136, 410, 558
MlyI GAGTC 1 cut(s) 34
MmeI TCCRAC 1 cut(s) 696
MseI TTAA 2 cut(s) 456, 564
MspI CCGG 2 cut(s) 37, 351
MspR9I CCNGG 2 cut(s) 38, 352
MwoI GCNNNNNNNGC 6 cut(s) 47, 56, 179, 440, 467, 599
NciI CCSGG 2 cut(s) 38, 352
NdeII GATC 5 cut(s) 427, 445, 586, 637, 709
NlaIII CATG 5 cut(s) 32, 189, 268, 310, 585
NspI RCATGY 3 cut(s) 268, 310, 585
PaeI GCATGC 1 cut(s) 585
PaqCI CACCTGC 1 cut(s) 410
PciI ACATGT 1 cut(s) 306
PfeI GAWTC 2 cut(s) 356, 644
PkrI GCNGC 4 cut(s) 442, 472, 487, 675
PleI GAGTC 1 cut(s) 33
PpsI GAGTC 1 cut(s) 33
PscI ACATGT 1 cut(s) 306
PspPI GGNCC 2 cut(s) 39, 523
PstNI CAGNNNCTG 1 cut(s) 440
PsuI RGATCY 1 cut(s) 709
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
SaqAI TTAA 2 cut(s) 456, 564
SatI GCNGC 4 cut(s) 441, 471, 486, 674
Sau3AI GATC 5 cut(s) 427, 445, 586, 637, 709
Sau96I GGNCC 2 cut(s) 39, 523
SchI GAGTC 1 cut(s) 34
ScrFI CCNGG 2 cut(s) 38, 352
SfaNI GCATC 2 cut(s) 191, 601
SphI GCATGC 1 cut(s) 585
Sse9I AATT 4 cut(s) 116, 136, 410, 558
SsiI CCGC 2 cut(s) 190, 485
StyD4I CCNGG 2 cut(s) 36, 350
StyI CCWWGG 1 cut(s) 225
TaaI ACNGT 4 cut(s) 124, 198, 364, 632
TaqI TCGA 1 cut(s) 359
TasI AATT 4 cut(s) 116, 136, 410, 558
TatI WGTACW 1 cut(s) 261
TauI GCSGC 1 cut(s) 488
TfiI GAWTC 2 cut(s) 356, 644
Tru1I TTAA 2 cut(s) 456, 564
Tru9I TTAA 2 cut(s) 456, 564
TscAI CASTG 1 cut(s) 321
TseI GCWGC 3 cut(s) 440, 470, 673
TspDTI ATGAA 6 cut(s) 87, 104, 333, 395, 570, 736
TspRI CASTG 1 cut(s) 321
XagI CCTNNNNNAGG 1 cut(s) 574
XapI RAATTY 3 cut(s) 136, 410, 558
XceI RCATGY 3 cut(s) 268, 310, 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.