Rh7BG448900

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0010696
Physical Location & Seq
Forward (+)
52450541 .. 52454276
3736 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG448900.1

Sequence Viewer

Length: 768 bp
ATGGAGAAGGATAGGGAGAGTCATGTTTACGCGGCCAAACTTGCTGAGCAAGCCGAAAGATATGATGAAATGATTGAAGTAATGAAAAAAGTTGCTAAGCTGGATGTGGAATTGACCGTGGATGAGCGAAATTTGTTGTCTGTTGGGTATAAGAATGTGATTGGGGCAAGAAGGGCATCATGGCGGATACTGTCTTCCATTGAACAGAAGGAGGAGGCCAAGGGAAATGAGCAGAATGTGAAGCGAATAAAGGAGTACATGCAAAGGGTTGAAGATGAGCTTGCAACTATTTGCAAGGACATGTTATCAGTGATTGATTATCACCTCCTCCCTTCTTCATCAACCGGGGAATCGACTGTATTCTACCATAAGATGAAAGGAGATTACTATCGTTATTTAGCTGAATTCAAAGCAGGTGATGATCGTGCAGAAGCTGCTGATCAGTCGCTTAAAGCCTATGAGGCTGCCAGTAGTAGTGCGGCCTCTCAGTTGCCCCCAACTCATCCAATCAGACTGGGCCTTGCTCTGAACTACTCAGTCTTTTACTATGAAATTCTTAAATCCTCTGAGAGGGCATGCGATCTTGCTAAGCAGGCATTTGATGATGCCGTTGCTGAGGTTGACAGTCTTGATCAAGAATCCTACAAGGACAGCACCCTCATTATGCAGCTTCTTAGGGATAATCTCACATTGTGGAACTCGGATCTGTCAGAGGGAGGAGGTAATCTTATGAGTCGTTTCATTTCCTTCCACCTTATTTTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

255

Amino Acids

29.04

Weight (kDa)

5.13

Isoelectric Point (pI)

48.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 12 - 233 5e-104 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010696)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22300 AT1G22300 AT1G22300 AT1G34760 AT1G34760
fragaria_vesca FvH4_5g37150
malus_domestica MD08G1184000.v1.1 MD15G1370500.v1.1
prunus_persica Prupe.1G518200_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0238741
rosa_laevigata RLG00000000816
rosa_multiflora Rmu_sc0010914.1_g000010
rosa_roxburghii Rroxscaffold_3G00221590
rosa_rugosa Rorug07G0323200.1 Rorug07G0323300.1
rosa_samantha Rh7AG477600 Rh7BG448900 Rh7CG495600 Rh7DG462600
rosa_wichuraiana Rw7G039860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 404
Acc36I ACCTGC 1 cut(s) 404
AccII CGCG 1 cut(s) 32
AciI CCGC 3 cut(s) 32, 184, 479
AclWI GGATC 1 cut(s) 711
AcoI YGGCCR 1 cut(s) 33
AcsI RAATTY 3 cut(s) 130, 404, 552
AdeI CACNNNGTG 1 cut(s) 693
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 1 cut(s) 570
AflIII ACRYGT 1 cut(s) 300
AgsI TTSAA 5 cut(s) 77, 203, 272, 409, 763
AluBI AGCT 5 cut(s) 100, 280, 401, 434, 670
AluI AGCT 5 cut(s) 100, 280, 401, 434, 670
AlwI GGATC 1 cut(s) 711
AlwNI CAGNNNCTG 1 cut(s) 434
AoxI GGCC 4 cut(s) 33, 216, 480, 517
ApeKI GCWGC 3 cut(s) 434, 464, 667
ApoI RAATTY 3 cut(s) 130, 404, 552
AspS9I GGNCC 1 cut(s) 517
AsuC2I CCSGG 1 cut(s) 346
AsuHPI GGTGA 2 cut(s) 314, 428
BbsI GAAGAC 1 cut(s) 186
BbvCI CCTCAGC 1 cut(s) 615
BbvI GCAGC 3 cut(s) 421, 451, 679
BceAI ACGGC 1 cut(s) 593
BciVI GTATCC 1 cut(s) 180
BclI TGATCA 2 cut(s) 439, 631
BcnI CCSGG 1 cut(s) 346
BfuAI ACCTGC 1 cut(s) 404
BfuI GTATCC 1 cut(s) 180
BglI GCCNNNNNGGC 1 cut(s) 461
BisI GCNGC 5 cut(s) 33, 435, 465, 480, 668
BlpI GCTNAGC 3 cut(s) 45, 96, 588
BlsI GCNGC 5 cut(s) 34, 436, 466, 481, 669
Bme1390I CCNGG 1 cut(s) 346
BmgT120I GGNCC 1 cut(s) 517
BmrFI CCNGG 1 cut(s) 346
BmrI ACTGGG 1 cut(s) 524
BmsI GCATC 2 cut(s) 185, 595
BmuI ACTGGG 1 cut(s) 524
BpiI GAAGAC 1 cut(s) 186
Bpu10I CCTNAGC 1 cut(s) 615
Bpu1102I GCTNAGC 3 cut(s) 45, 96, 588
BpuMI CCSGG 1 cut(s) 346
BsaBI GATNNNNATC 1 cut(s) 387
BsaJI CCNNGG 3 cut(s) 117, 219, 345
Bsc4I CCNNNNNNNGG 1 cut(s) 570
Bse1I ACTGG 2 cut(s) 468, 519
Bse8I GATNNNNATC 1 cut(s) 387
BseDI CCNNGG 3 cut(s) 117, 219, 345
BseGI GGATG 3 cut(s) 109, 127, 502
BseJI GATNNNNATC 1 cut(s) 387
BseLI CCNNNNNNNGG 1 cut(s) 570
BseMII CTCAG 5 cut(s) 36, 500, 549, 558, 606
BseNI ACTGG 2 cut(s) 468, 519
BseRI GAGGAG 3 cut(s) 227, 317, 732
BseXI GCAGC 3 cut(s) 421, 451, 679
BsgI GTGCAG 1 cut(s) 447
Bsh1236I CGCG 1 cut(s) 32
BshFI GGCC 4 cut(s) 35, 218, 482, 519
BsiSI CCGG 1 cut(s) 345
BslI CCNNNNNNNGG 1 cut(s) 570
BsnI GGCC 4 cut(s) 35, 218, 482, 519
Bsp143I GATC 5 cut(s) 421, 439, 580, 631, 703
Bsp1720I GCTNAGC 3 cut(s) 45, 96, 588
BspACI CCGC 3 cut(s) 32, 184, 479
BspANI GGCC 4 cut(s) 35, 218, 482, 519
BspCNI CTCAG 5 cut(s) 37, 499, 548, 559, 607
BspFNI CGCG 1 cut(s) 32
BspMI ACCTGC 1 cut(s) 404
BspPI GGATC 1 cut(s) 711
BsrI ACTGG 2 cut(s) 468, 519
BssECI CCNNGG 3 cut(s) 117, 219, 345
BssMI GATC 5 cut(s) 421, 439, 580, 631, 703
BssT1I CCWWGG 1 cut(s) 219
Bst4CI ACNGT 4 cut(s) 118, 192, 358, 626
BstAPI GCANNNNNTGC 1 cut(s) 434
BstC8I GCNNGC 4 cut(s) 51, 282, 577, 594
BstDEI CTNAG 8 cut(s) 45, 96, 486, 535, 567, 588, 615, 674
BstDSI CCRYGG 1 cut(s) 117
BstENI CCTNNNNNAGG 1 cut(s) 568
BstF5I GGATG 3 cut(s) 109, 127, 502
BstFNI CGCG 1 cut(s) 32
BstKTI GATC 5 cut(s) 424, 442, 583, 634, 706
BstMBI GATC 5 cut(s) 421, 439, 580, 631, 703
BstMWI GCNNNNNNNGC 6 cut(s) 41, 50, 173, 434, 461, 593
BstNSI RCATGY 3 cut(s) 262, 304, 579
BstSCI CCNGG 1 cut(s) 344
BstUI CGCG 1 cut(s) 32
BstV1I GCAGC 3 cut(s) 421, 451, 679
BstV2I GAAGAC 1 cut(s) 186
BstX2I RGATCY 1 cut(s) 703
BstYI RGATCY 1 cut(s) 703
BsuI GTATCC 1 cut(s) 180
BsuRI GGCC 4 cut(s) 35, 218, 482, 519
BtgI CCRYGG 1 cut(s) 117
BtsCI GGATG 3 cut(s) 109, 127, 502
BtsIMutI CAGTG 1 cut(s) 315
BveI ACCTGC 1 cut(s) 404
Cac8I GCNNGC 4 cut(s) 51, 282, 577, 594
CaiI CAGNNNCTG 1 cut(s) 434
Cfr13I GGNCC 1 cut(s) 517
Csp6I GTAC 1 cut(s) 256
CviAII CATG 5 cut(s) 23, 180, 259, 301, 576
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 8 cut(s) 45, 96, 486, 535, 567, 588, 615, 674
DpnI GATC 5 cut(s) 423, 441, 582, 633, 705
DpnII GATC 5 cut(s) 421, 439, 580, 631, 703
DraIII CACNNNGTG 1 cut(s) 693
EaeI YGGCCR 1 cut(s) 33
EciI GGCGGA 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 219
EcoNI CCTNNNNNAGG 1 cut(s) 568
EcoRI GAATTC 1 cut(s) 404
EcoT14I CCWWGG 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 5 cut(s) 26, 183, 262, 304, 579
FalI AAGNNNNNCTT 2 cut(s) 264, 296
FatI CATG 5 cut(s) 22, 179, 258, 300, 575
FbaI TGATCA 2 cut(s) 439, 631
Fnu4HI GCNGC 5 cut(s) 33, 435, 465, 480, 668
FokI GGATG 3 cut(s) 116, 134, 489
Fsp4HI GCNGC 5 cut(s) 33, 435, 465, 480, 668
GluI GCNGC 5 cut(s) 33, 435, 465, 480, 668
HaeIII GGCC 4 cut(s) 35, 218, 482, 519
HapII CCGG 1 cut(s) 345
Hin1II CATG 5 cut(s) 26, 183, 262, 304, 579
HincII GTYRAC 1 cut(s) 622
HindII GTYRAC 1 cut(s) 622
HinfI GANTC 4 cut(s) 19, 350, 638, 733
HpaII CCGG 1 cut(s) 345
HphI GGTGA 2 cut(s) 314, 428
Hpy166II GTNNAC 2 cut(s) 28, 622
Hpy188I TCNGA 5 cut(s) 512, 528, 568, 703, 712
Hpy188III TCNNGA 2 cut(s) 629, 635
Hpy8I GTNNAC 2 cut(s) 28, 622
HpyAV CCTTC 4 cut(s) 165, 202, 342, 757
HpyCH4III ACNGT 4 cut(s) 118, 192, 358, 626
HpyCH4V TGCA 5 cut(s) 262, 284, 294, 428, 667
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 50, 173, 434, 461, 593
HpyF3I CTNAG 8 cut(s) 45, 96, 486, 535, 567, 588, 615, 674
Hsp92II CATG 5 cut(s) 26, 183, 262, 304, 579
Ksp22I TGATCA 2 cut(s) 439, 631
Kzo9I GATC 5 cut(s) 421, 439, 580, 631, 703
LpnPI CCDG 6 cut(s) 86, 358, 399, 481, 500, 578
Lsp1109I GCAGC 3 cut(s) 421, 451, 679
LweI GCATC 2 cut(s) 185, 595
MalI GATC 5 cut(s) 423, 441, 582, 633, 705
MboI GATC 5 cut(s) 421, 439, 580, 631, 703
MboII GAAGA 3 cut(s) 186, 284, 327
MflI RGATCY 1 cut(s) 703
MluCI AATT 4 cut(s) 110, 130, 404, 552
MlyI GAGTC 2 cut(s) 28, 742
MseI TTAA 2 cut(s) 450, 558
MspI CCGG 1 cut(s) 345
MspR9I CCNGG 1 cut(s) 346
MvnI CGCG 1 cut(s) 32
MwoI GCNNNNNNNGC 6 cut(s) 41, 50, 173, 434, 461, 593
NciI CCSGG 1 cut(s) 346
NdeII GATC 5 cut(s) 421, 439, 580, 631, 703
NlaIII CATG 5 cut(s) 26, 183, 262, 304, 579
NspI RCATGY 3 cut(s) 262, 304, 579
PaeI GCATGC 1 cut(s) 579
PaqCI CACCTGC 1 cut(s) 404
PciI ACATGT 1 cut(s) 300
PfeI GAWTC 2 cut(s) 350, 638
PkrI GCNGC 5 cut(s) 34, 436, 466, 481, 669
PleI GAGTC 2 cut(s) 27, 741
PpsI GAGTC 2 cut(s) 27, 741
PscI ACATGT 1 cut(s) 300
PspPI GGNCC 1 cut(s) 517
PstNI CAGNNNCTG 1 cut(s) 434
PsuI RGATCY 1 cut(s) 703
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
SaqAI TTAA 2 cut(s) 450, 558
SatI GCNGC 5 cut(s) 33, 435, 465, 480, 668
Sau3AI GATC 5 cut(s) 421, 439, 580, 631, 703
Sau96I GGNCC 1 cut(s) 517
SchI GAGTC 2 cut(s) 28, 742
ScrFI CCNGG 1 cut(s) 346
SfaNI GCATC 2 cut(s) 185, 595
SphI GCATGC 1 cut(s) 579
Sse9I AATT 4 cut(s) 110, 130, 404, 552
SsiI CCGC 3 cut(s) 32, 184, 479
StyD4I CCNGG 1 cut(s) 344
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 4 cut(s) 118, 192, 358, 626
TaqI TCGA 1 cut(s) 353
TasI AATT 4 cut(s) 110, 130, 404, 552
TatI WGTACW 1 cut(s) 255
TauI GCSGC 2 cut(s) 35, 482
TfiI GAWTC 2 cut(s) 350, 638
Tru1I TTAA 2 cut(s) 450, 558
Tru9I TTAA 2 cut(s) 450, 558
TscAI CASTG 1 cut(s) 315
TseI GCWGC 3 cut(s) 434, 464, 667
TspDTI ATGAA 6 cut(s) 81, 98, 327, 389, 564, 730
TspRI CASTG 1 cut(s) 315
XagI CCTNNNNNAGG 1 cut(s) 568
XapI RAATTY 3 cut(s) 130, 404, 552
XceI RCATGY 3 cut(s) 262, 304, 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.