Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0004358

Physical Location & Seq

Genomic Coordinates Forward (+)

26571239 .. 26578888

Gene Structure 7650 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1205100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2643 bp

ATGGAAAACTCAGACTCTGACGAGCCAGAGAAGAAGAGGCCTCATTTGAACTCTCTTTCCCCCACCATGGCCCGTAGCTCCTCCACCTCTCCGCTCAAGAACCGCAGCGTCGATGCAGCAGCTCTCCAGCTCCAGAACCAGCAACTTAATCAGCAGGTAGACAAACAGAAGCTTGACTTGCAAGATCTTGAAGCAAAGATGCAGGAATTAAAGGATAAGCAAGGTTCTTATGATGATTTCTTAATCACAATGAATCAACTATGGAATCAGTTGGTTGATGATCTAATTCTTCTTGGACTATGTGCTGGAGGAGACCAAAATGCTTTAGAAATTTTAGATGGTGCTGATTATTCTCGTGGTTCAATTCCATCATGTTCTGCGGAAGAGATGTTTCTTTTTAGACTCCTACAACAAGATTCTCTAGAAGCTAATGGCAATGATGAAATTGTTAAATATGTTGAAGAAACTCTCGCTTTGCGGCATACGTCTACTATGAACTTGTTAGAACTCTTAGAACATGCCATTCATGCTCATAGGGAAAAAACAATGAACATATCTCATACTTTGGATGGAAAGATATCTTCAGAAGATGCTATCATCCAGTTTTCTAAAATTGATGATATGATGGAAAGGGAGGTTAAAAATCTACGCGAAGGGATTGATTTTCTTCATCTGAAGCATAAAGAGTATGCTAATGTAATTCGGACTTACCTCAGCAGCCATTCAAGAGATCAGTCTGAAATTAGGCGCATCACAGGTGAGCTGGATGACAAAATGGCTGACCTAGAGTTGAAGAGAAGGAAACTAATTAATCTGAAAATGCAAAAGGATGTTGGACCTGGGGTGCAGAACCATACTTCTAGTGCACTTAATGGAACCCCGTCCCCTGAAAAATCAACAGAAAGGACAATCAACTTGCAAGAACTTAGAAATTCAATTGAGGAAACAAGGATACTAGAAGCCGACCGCCTTTCTGAGTATCAAGAGGCAAATGAAGAAAATTTAGCACTGTCAAAACAATTGCAAGAATTTCAGGATGAAGTGAAGGATGACAAATATGTACTTTCATCAAGACTGTACTCTATGCTAAACGATCAACTCCAACATTGGAATGCAGAATTGGACCGATACAAAGCATTGACTGATTCTTTGCAGGCTGACAGGACCCTTGTTGTGAGAAGGGAGAAGGACCTAAATGTCAAATTAGAGTCAGCAGATGCAGTTAGGAATGCCATTGACAATTCTGATTCTAGGATTGAAGAACTAGAACTTCAGCTGCAGAAGTGTATAATTGCAAAAAATGACTTTGAGATAAACATGGAAGAAGCTGTCCAGGATTCAGGAAGAAAGGACATTATAGCAGAGTTTCGTGTGATGGCCTCATCTTTGTGTAAAGAAATGGGAATGGTGGAAACTCAGTTGAAGCGGTGGAAGGAAACAGCTCATGAAACTGTTTCTTTACGTGACAAAGCCCAATCATTGAAAGCTTCATTAAGTACAAAGACAAATGAGCAAAAGATCCTTGTGGACAAGTGTGGTGAACAGGTGATAGAGATAAAGTCTCTTAAGGCATTGATTGAGAAGTTGCAGAAAGAAAAACTGGAACTGCAGATCTTCTTAGACATGTATGTACAGGAAAGCTATGAAAACAGGGACTTGATGGAAATCAAAGAATCAGAACGCAGAGCTTACTTTCAAGCTGAAATGCTCAAGAATGCATTGGATGAACATAGTCTAGAGTTGAGGGTGAAAGCAGCCAATGAAGCAGAAGCTGCTTGTCAGCAAAGGCTTTCTGCTACCGAAGCTGAAATAACTGAATTAAGTGCCAAACTTGATGCATCAGAGCGGGATGTTATGGAGCTTACTGAGGCTATTGGAATTAAAGACAAGGAGGCAGAGGCTTATATATCTGAAATTGAGACCATTGGTCAGGCATATGAAGATATGCAGATGCAAAATCAGCATCTGTTGCAGCAGGTGACTGAGAGGGATGACTACAATATCAAGCTCGTTTCTGAGAGTGTGAAGGCAAAGCAAGCACAGAGCTTTGTAGTCTCCGATAAGCAGGCATTGGCGAAGCAACTTCAACAAGTAAACACATCATTAGAATCTCTAAAAATTAGAGTTTCCCATGGTGAAGAGCAGATGATGGCTCTCCTGATAGATGCCATAAAAACTGCTGAGGAAGATAGGCAACTCGCACTCAACGTGGAAACTGTCAAGTGGGAATTGGCTGATTCTGAGAAGGAGTTGCAGTGGTTGAAATCTGCCTTTGCATCTTCTGAGAAGGAACATGTGCATATTGAAAAGGATATTGATGACATCCGATTGGAATTAGACAATGAAAGAAGCTCAAGGAAGAACCTTGAGGAACAACTCGAGGAATTGAACGGCATAGTTGCTGAGATGAGTTCTGAAACCGGAGAGGCTGCAATACAGAAACTCCAAACTGAAATAAAATTCTGCAAAAATATTTTGCAGTGCAGCGTTTGTACTGATAGGCCGAAGGAGGTGGTAATCGTTAAGTGTTATCATCTATTTTGCAATTACTGCGTTCAAAAAAATCTAGAGATACGACATCGAAAGTGTCCTGCCTGTGGAACTCCGTTTGGTCAAAATGACATCCGGTTTGTAAAGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0002376 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006464 GO:0006513 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009791 GO:0009965 GO:0009987 GO:0010016 GO:0010154 GO:0010162 GO:0010228 GO:0010390 GO:0010431 GO:0016043 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0019538 GO:0021700 GO:0022414 GO:0022611 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0033523 GO:0036211 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045087 GO:0046983 GO:0048316 GO:0048366 GO:0048367 GO:0048608 GO:0048609 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051276 GO:0061458 GO:0070647 GO:0071695 GO:0071704 GO:0071840 GO:0099402 GO:1901564 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_3

Protein Analysis

881

Amino Acids

100.87

Weight (kDa)

4.96

Isoelectric Point (pI)

51.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_3	PF13920	826 - 869	4.8e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2	PF13923	827 - 866	5e-06	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	828 - 866	3.3e-08	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004358)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55250 AT1G55250 AT1G55250 AT1G55250 AT1G55250
fragaria_vesca	FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811
malus_domestica	MD08G1205100.v1.1 MD15G1388900.v1.1
prunus_persica	Prupe.1G537100_v2.0.a1
pyrus_communis	pycom08g17730 pycom15g34870
rosa_chinensis	RchiOBHm_Chr5g0081511
rosa_laevigata	RLG00000036943
rosa_multiflora	Rmu_sc0007702.1_g000003
rosa_roxburghii	Rroxscaffold_1G00002040
rosa_rugosa	Rorug05G0476800
rosa_samantha	Rh5AG528300 Rh5BG553000 Rh5CG575900 Rh5DG563100
rosa_wichuraiana	Rw5G049140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1966
AasI	GACNNNNNNGTC	1 cut(s)	1196
Acc36I	ACCTGC	2 cut(s)	145, 1966
AccBSI	CCGCTC	2 cut(s)	94, 1846
AccI	GTMKAC	2 cut(s)	159, 488
AccII	CGCG	1 cut(s)	651
AciI	CCGC	7 cut(s)	92, 103, 380, 478, 967, 1426, 1846
AclWI	GGATC	1 cut(s)	1513
AcsI	RAATTY	5 cut(s)	330, 931, 1000, 1028, 2459
AcuI	CTGAAG	3 cut(s)	567, 695, 1256
AfaI	GTAC	5 cut(s)	1062, 1079, 1498, 1632, 2494
AfiI	CCNNNNNNNGG	2 cut(s)	67, 2597
AflII	CTTAAG	1 cut(s)	1565
AflIII	ACRYGT	2 cut(s)	1623, 2293
AhdI	GACNNNNNGTC	1 cut(s)	1926
AjnI	CCWGG	2 cut(s)	838, 1332
AjuI	GAANNNNNNNTTGG	2 cut(s)	1103, 1135
AloI	GAACNNNNNNTCC	2 cut(s)	41, 73
Alw21I	GWGCWC	1 cut(s)	868
Alw26I	GTCTC	4 cut(s)	306, 1566, 1913, 2059
Alw44I	GTGCAC	1 cut(s)	864
AlwI	GGATC	1 cut(s)	1513
AlwNI	CAGNNNCTG	3 cut(s)	17, 1772, 1966
Ama87I	CYCGRG	1 cut(s)	2378
AoxI	GGCC	4 cut(s)	38, 69, 1377, 2501
ApaLI	GTGCAC	1 cut(s)	864
ApoI	RAATTY	5 cut(s)	330, 931, 1000, 1028, 2459
ArsI	GACNNNNNNTTYG	3 cut(s)	1132, 1164, 2612
AseI	ATTAAT	1 cut(s)	810
AspLEI	GCGC	1 cut(s)	750
AspS9I	GGNCC	5 cut(s)	70, 836, 1123, 1164, 1189
AsuHPI	GGTGA	6 cut(s)	770, 1550, 1558, 1759, 1990, 2147
AvaI	CYCGRG	1 cut(s)	2378
AvaII	GGWCC	4 cut(s)	836, 1123, 1164, 1189
BaeGI	GKGCMC	1 cut(s)	868
BauI	CACGAG	1 cut(s)	354
Bbv12I	GWGCWC	1 cut(s)	868
BbvCI	CCTCAGC	2 cut(s)	713, 2181
BccI	CCATC	7 cut(s)	332, 376, 563, 619, 1369, 1654, 2143
BceAI	ACGGC	1 cut(s)	2407
BciT130I	CCWGG	2 cut(s)	840, 1334
BciVI	GTATCC	1 cut(s)	945
BcoDI	GTCTC	4 cut(s)	306, 1566, 1913, 2059
BfaI	CTAG	8 cut(s)	422, 785, 861, 956, 1251, 1265, 1736, 2567
BfmI	CTRYAG	2 cut(s)	1277, 1607
BfrI	CTTAAG	1 cut(s)	1565
BfuAI	ACCTGC	2 cut(s)	145, 1966
BfuI	GTATCC	1 cut(s)	945
BglII	AGATCT	2 cut(s)	184, 1611
Bme1390I	CCNGG	2 cut(s)	840, 1334
Bme18I	GGWCC	4 cut(s)	836, 1123, 1164, 1189
BmeRI	GACNNNNNGTC	1 cut(s)	1926
BmeT110I	CYCGRG	1 cut(s)	2378
BmgT120I	GGNCC	5 cut(s)	70, 836, 1123, 1164, 1189
BmiI	GGNNCC	2 cut(s)	877, 1166
BmrFI	CCNGG	2 cut(s)	840, 1334
BpmI	CTGGAG	3 cut(s)	110, 116, 327
Bpu10I	CCTNAGC	2 cut(s)	713, 2181
BpuEI	CTTGAG	4 cut(s)	80, 1694, 2338, 2387
BsaAI	YACGTR	1 cut(s)	1463
BsaBI	GATNNNNATC	1 cut(s)	1664
BsaI	GGTCTC	2 cut(s)	306, 1913
BsaJI	CCNNGG	3 cut(s)	66, 839, 2131
BsaWI	WCCGGW	2 cut(s)	2420, 2625
BsaXI	ACNNNNNCTCC	4 cut(s)	2240, 2270, 2427, 2457
Bsc4I	CCNNNNNNNGG	2 cut(s)	67, 2597
Bse1I	ACTGG	2 cut(s)	601, 1605
Bse3DI	GCAATG	1 cut(s)	442
Bse8I	GATNNNNATC	1 cut(s)	1664
BseBI	CCWGG	2 cut(s)	840, 1334
BseDI	CCNNGG	3 cut(s)	66, 839, 2131
BseJI	GATNNNNATC	1 cut(s)	1664
BseLI	CCNNNNNNNGG	2 cut(s)	67, 2597
BseMI	GCAATG	1 cut(s)	442
BseNI	ACTGG	2 cut(s)	601, 1605
BseRI	GAGGAG	2 cut(s)	70, 324
BseSI	GKGCMC	1 cut(s)	868
BsgI	GTGCAG	2 cut(s)	866, 2503
Bsh1236I	CGCG	1 cut(s)	651
Bsh1285I	CGRYCG	1 cut(s)	967
BshFI	GGCC	4 cut(s)	40, 71, 1379, 2503
BsiEI	CGRYCG	1 cut(s)	967
BsiHKAI	GWGCWC	1 cut(s)	868
BsiHKCI	CYCGRG	1 cut(s)	2378
BsiSI	CCGG	2 cut(s)	2421, 2626
BslFI	GGGAC	2 cut(s)	868, 1667
BslI	CCNNNNNNNGG	2 cut(s)	67, 2597
BsmAI	GTCTC	4 cut(s)	306, 1566, 1913, 2059
BsmFI	GGGAC	2 cut(s)	868, 1667
BsmI	GAATGC	3 cut(s)	1117, 1234, 1720
BsnI	GGCC	4 cut(s)	40, 71, 1379, 2503
Bso31I	GGTCTC	2 cut(s)	306, 1913
BsoBI	CYCGRG	1 cut(s)	2378
Bsp1286I	GDGCHC	1 cut(s)	868
Bsp1407I	TGTACA	1 cut(s)	1630
Bsp143I	GATC	6 cut(s)	184, 280, 730, 1093, 1518, 1611
Bsp19I	CCATGG	2 cut(s)	66, 2131
BspACI	CCGC	7 cut(s)	92, 103, 380, 478, 967, 1426, 1846
BspANI	GGCC	4 cut(s)	40, 71, 1379, 2503
BspFNI	CGCG	1 cut(s)	651
BspHI	TCATGA	1 cut(s)	1444
BspLI	GGNNCC	2 cut(s)	877, 1166
BspMAI	CTGCAG	2 cut(s)	1281, 1611
BspMI	ACCTGC	2 cut(s)	145, 1966
BspPI	GGATC	1 cut(s)	1513
BspQI	GCTCTTC	1 cut(s)	2133
BspTI	CTTAAG	1 cut(s)	1565
BspTNI	GGTCTC	2 cut(s)	306, 1913
BsrBI	CCGCTC	2 cut(s)	94, 1846
BsrDI	GCAATG	1 cut(s)	442
BsrGI	TGTACA	1 cut(s)	1630
BsrI	ACTGG	2 cut(s)	601, 1605
BssECI	CCNNGG	3 cut(s)	66, 839, 2131
BssMI	GATC	6 cut(s)	184, 280, 730, 1093, 1518, 1611
BssSI	CACGAG	1 cut(s)	354
BssT1I	CCWWGG	2 cut(s)	66, 2131
Bst2BI	CACGAG	1 cut(s)	354
Bst2UI	CCWGG	2 cut(s)	840, 1334
Bst4CI	ACNGT	4 cut(s)	1011, 1077, 1453, 2218
Bst6I	CTCTTC	4 cut(s)	29, 378, 788, 2133
BstAFI	CTTAAG	1 cut(s)	1565
BstAPI	GCANNNNNTGC	3 cut(s)	1772, 1969, 2550
BstAUI	TGTACA	1 cut(s)	1630
BstBAI	YACGTR	1 cut(s)	1463
BstC8I	GCNNGC	3 cut(s)	1155, 2037, 2067
BstDSI	CCRYGG	2 cut(s)	66, 2131
BstFNI	CGCG	1 cut(s)	651
BstHHI	GCGC	1 cut(s)	750
BstKTI	GATC	6 cut(s)	187, 283, 733, 1096, 1521, 1614
BstMAI	GTCTC	4 cut(s)	306, 1566, 1913, 2059
BstMBI	GATC	6 cut(s)	184, 280, 730, 1093, 1518, 1611
BstMCI	CGRYCG	1 cut(s)	967
BstMWI	GCNNNNNNNGC	9 cut(s)	178, 527, 1763, 1772, 1802, 1960, 1969, 2036, 2550
BstNI	CCWGG	2 cut(s)	840, 1334
BstNSI	RCATGY	3 cut(s)	521, 1627, 2297
BstSCI	CCNGG	2 cut(s)	838, 1332
BstSFI	CTRYAG	2 cut(s)	1277, 1607
BstSLI	GKGCMC	1 cut(s)	868
BstUI	CGCG	1 cut(s)	651
BstX2I	RGATCY	3 cut(s)	184, 1518, 1611
BstYI	RGATCY	3 cut(s)	184, 1518, 1611
BsuI	GTATCC	1 cut(s)	945
BsuRI	GGCC	4 cut(s)	40, 71, 1379, 2503
BtgI	CCRYGG	2 cut(s)	66, 2131
BtsI	GCAGTG	2 cut(s)	2261, 2486
BtsIMutI	CAGTG	3 cut(s)	1007, 2261, 2486
BveI	ACCTGC	2 cut(s)	145, 1966
Cac8I	GCNNGC	3 cut(s)	1155, 2037, 2067
CaiI	CAGNNNCTG	3 cut(s)	17, 1772, 1966
CciI	TCATGA	1 cut(s)	1444
CfoI	GCGC	1 cut(s)	750
Cfr13I	GGNCC	5 cut(s)	70, 836, 1123, 1164, 1189
CseI	GACGC	1 cut(s)	97
Csp6I	GTAC	5 cut(s)	1061, 1078, 1497, 1631, 2493
CviAII	CATG	9 cut(s)	67, 372, 518, 527, 1318, 1445, 1624, 2132, 2294
CviQI	GTAC	5 cut(s)	1061, 1078, 1497, 1631, 2493
DpnI	GATC	6 cut(s)	186, 282, 732, 1095, 1520, 1613
DpnII	GATC	6 cut(s)	184, 280, 730, 1093, 1518, 1611
DrdI	GACNNNNNNGTC	1 cut(s)	1196
DriI	GACNNNNNGTC	1 cut(s)	1926
DseDI	GACNNNNNNGTC	1 cut(s)	1196
Eam1104I	CTCTTC	4 cut(s)	29, 378, 788, 2133
Eam1105I	GACNNNNNGTC	1 cut(s)	1926
EarI	CTCTTC	4 cut(s)	29, 378, 788, 2133
Eco130I	CCWWGG	2 cut(s)	66, 2131
Eco147I	AGGCCT	1 cut(s)	40
Eco31I	GGTCTC	2 cut(s)	306, 1913
Eco32I	GATATC	1 cut(s)	579
Eco47I	GGWCC	4 cut(s)	836, 1123, 1164, 1189
Eco57I	CTGAAG	3 cut(s)	567, 695, 1256
Eco88I	CYCGRG	1 cut(s)	2378
EcoO109I	RGGNCCY	2 cut(s)	1164, 1189
EcoRII	CCWGG	2 cut(s)	838, 1332
EcoRV	GATATC	1 cut(s)	579
EcoT14I	CCWWGG	2 cut(s)	66, 2131
EcoT22I	ATGCAT	2 cut(s)	1720, 1840
ErhI	CCWWGG	2 cut(s)	66, 2131
FaeI	CATG	9 cut(s)	70, 375, 521, 530, 1321, 1448, 1627, 2135, 2297
FalI	AAGNNNNNCTT	2 cut(s)	161, 193
FaqI	GGGAC	2 cut(s)	868, 1667
FatI	CATG	9 cut(s)	66, 371, 517, 526, 1317, 1444, 1623, 2131, 2293
FauI	CCCGC	1 cut(s)	1839
FauNDI	CATATG	1 cut(s)	1936
FblI	GTMKAC	2 cut(s)	159, 488
FspBI	CTAG	8 cut(s)	422, 785, 861, 956, 1251, 1265, 1736, 2567
GlaI	GCGC	1 cut(s)	749
GsuI	CTGGAG	3 cut(s)	110, 116, 327
HaeIII	GGCC	4 cut(s)	40, 71, 1379, 2503
HapII	CCGG	2 cut(s)	2421, 2626
HgaI	GACGC	1 cut(s)	97
HhaI	GCGC	1 cut(s)	750
Hin1II	CATG	9 cut(s)	70, 375, 521, 530, 1321, 1448, 1627, 2135, 2297
Hin6I	GCGC	1 cut(s)	748
HinP1I	GCGC	1 cut(s)	748
HindIII	AAGCTT	2 cut(s)	170, 1485
HpaII	CCGG	2 cut(s)	2421, 2626
HphI	GGTGA	6 cut(s)	770, 1550, 1558, 1759, 1990, 2147
Hpy166II	GTNNAC	6 cut(s)	160, 489, 866, 1528, 1541, 2095
Hpy8I	GTNNAC	6 cut(s)	160, 489, 866, 1528, 1541, 2095
Hpy99I	CGWCG	1 cut(s)	113
HpyCH4III	ACNGT	4 cut(s)	1011, 1077, 1453, 2218
HpyCH4IV	ACGT	3 cut(s)	485, 1462, 2208
HpyF10VI	GCNNNNNNNGC	9 cut(s)	178, 527, 1763, 1772, 1802, 1960, 1969, 2036, 2550
HpySE526I	ACGT	3 cut(s)	485, 1462, 2208
Hsp92II	CATG	9 cut(s)	70, 375, 521, 530, 1321, 1448, 1627, 2135, 2297
HspAI	GCGC	1 cut(s)	748
Kzo9I	GATC	6 cut(s)	184, 280, 730, 1093, 1518, 1611
LguI	GCTCTTC	1 cut(s)	2133
LmnI	GCTCC	3 cut(s)	83, 135, 1858
MaeI	CTAG	8 cut(s)	422, 785, 861, 956, 1251, 1265, 1736, 2567
MaeII	ACGT	3 cut(s)	485, 1462, 2208
MaeIII	GTNAC	2 cut(s)	1463, 1978
MalI	GATC	6 cut(s)	186, 282, 732, 1095, 1520, 1613
MbiI	CCGCTC	2 cut(s)	94, 1846
MboI	GATC	6 cut(s)	184, 280, 730, 1093, 1518, 1611
MfeI	CAATTG	2 cut(s)	936, 1019
MflI	RGATCY	3 cut(s)	184, 1518, 1611
MhlI	GDGCHC	1 cut(s)	868
MlyI	GAGTC	3 cut(s)	8, 396, 1217
MmeI	TCCRAC	2 cut(s)	814, 1126
Mph1103I	ATGCAT	2 cut(s)	1720, 1840
MslI	CAYNNNNRTG	2 cut(s)	1110, 1387
MspA1I	CMGCKG	1 cut(s)	1276
MspCI	CTTAAG	1 cut(s)	1565
MspI	CCGG	2 cut(s)	2421, 2626
MspR9I	CCNGG	2 cut(s)	840, 1334
MunI	CAATTG	2 cut(s)	936, 1019
Mva1269I	GAATGC	3 cut(s)	1117, 1234, 1720
MvaI	CCWGG	2 cut(s)	840, 1334
MvnI	CGCG	1 cut(s)	651
MwoI	GCNNNNNNNGC	9 cut(s)	178, 527, 1763, 1772, 1802, 1960, 1969, 2036, 2550
NcoI	CCATGG	2 cut(s)	66, 2131
NdeI	CATATG	1 cut(s)	1936
NdeII	GATC	6 cut(s)	184, 280, 730, 1093, 1518, 1611
NlaIII	CATG	9 cut(s)	70, 375, 521, 530, 1321, 1448, 1627, 2135, 2297
NlaIV	GGNNCC	2 cut(s)	877, 1166
NmuCI	GTSAC	2 cut(s)	1463, 1978
NsiI	ATGCAT	2 cut(s)	1720, 1840
NspI	RCATGY	3 cut(s)	521, 1627, 2297
PaeR7I	CTCGAG	1 cut(s)	2378
PagI	TCATGA	1 cut(s)	1444
PaqCI	CACCTGC	1 cut(s)	1966
PceI	AGGCCT	1 cut(s)	40
PciI	ACATGT	2 cut(s)	1623, 2293
PciSI	GCTCTTC	1 cut(s)	2133
PcsI	WCGNNNNNNNCGW	1 cut(s)	2205
PctI	GAATGC	3 cut(s)	1117, 1234, 1720
PfeI	GAWTC	9 cut(s)	253, 265, 416, 1145, 1247, 1337, 1673, 2108, 2237
PfoI	TCCNGGA	1 cut(s)	1332
PleI	GAGTC	3 cut(s)	8, 396, 1216
PpsI	GAGTC	3 cut(s)	8, 396, 1216
Ppu21I	YACGTR	1 cut(s)	1463
PpuMI	RGGWCCY	2 cut(s)	1164, 1189
PscI	ACATGT	2 cut(s)	1623, 2293
PshBI	ATTAAT	1 cut(s)	810
Psp5II	RGGWCCY	2 cut(s)	1164, 1189
Psp6I	CCWGG	2 cut(s)	838, 1332
PspGI	CCWGG	2 cut(s)	838, 1332
PspN4I	GGNNCC	2 cut(s)	877, 1166
PspPI	GGNCC	5 cut(s)	70, 836, 1123, 1164, 1189
PspPPI	RGGWCCY	2 cut(s)	1164, 1189
PspXI	VCTCGAGB	1 cut(s)	2378
PstI	CTGCAG	2 cut(s)	1281, 1611
PstNI	CAGNNNCTG	3 cut(s)	17, 1772, 1966
PsuI	RGATCY	3 cut(s)	184, 1518, 1611
PvuII	CAGCTG	1 cut(s)	1276
RsaI	GTAC	5 cut(s)	1062, 1079, 1498, 1632, 2494
RsaNI	GTAC	5 cut(s)	1061, 1078, 1497, 1631, 2493
RseI	CAYNNNNRTG	2 cut(s)	1110, 1387
SapI	GCTCTTC	1 cut(s)	2133
Sau3AI	GATC	6 cut(s)	184, 280, 730, 1093, 1518, 1611
Sau96I	GGNCC	5 cut(s)	70, 836, 1123, 1164, 1189
SchI	GAGTC	3 cut(s)	8, 396, 1217
ScrFI	CCNGG	2 cut(s)	840, 1334
SduI	GDGCHC	1 cut(s)	868
SfcI	CTRYAG	2 cut(s)	1277, 1607
Sfr274I	CTCGAG	1 cut(s)	2378
SinI	GGWCC	4 cut(s)	836, 1123, 1164, 1189
SlaI	CTCGAG	1 cut(s)	2378
SmiMI	CAYNNNNRTG	2 cut(s)	1110, 1387
SmlI	CTYRAG	6 cut(s)	95, 1565, 1709, 2353, 2366, 2378
SmoI	CTYRAG	6 cut(s)	95, 1565, 1709, 2353, 2366, 2378
SseBI	AGGCCT	1 cut(s)	40
SsiI	CCGC	7 cut(s)	92, 103, 380, 478, 967, 1426, 1846
SspI	AATATT	1 cut(s)	2473
SspMI	CTAG	8 cut(s)	422, 785, 861, 956, 1251, 1265, 1736, 2567
StuI	AGGCCT	1 cut(s)	40
StyD4I	CCNGG	2 cut(s)	838, 1332
StyI	CCWWGG	2 cut(s)	66, 2131
TaaI	ACNGT	4 cut(s)	1011, 1077, 1453, 2218
TaiI	ACGT	3 cut(s)	488, 1465, 2211
TaqI	TCGA	3 cut(s)	111, 2379, 2581
TaqII	GACCGA	1 cut(s)	1140
TatI	WGTACW	5 cut(s)	1060, 1077, 1496, 1630, 2492
TauI	GCSGC	1 cut(s)	481
TfiI	GAWTC	9 cut(s)	253, 265, 416, 1145, 1247, 1337, 1673, 2108, 2237
TscAI	CASTG	3 cut(s)	1014, 2261, 2486
TseFI	GTSAC	2 cut(s)	1463, 1978
Tsp45I	GTSAC	2 cut(s)	1463, 1978
TspGWI	ACGGA	1 cut(s)	2595
TspRI	CASTG	3 cut(s)	1014, 2261, 2486
Vha464I	CTTAAG	1 cut(s)	1565
VneI	GTGCAC	1 cut(s)	864
VpaK11BI	GGWCC	4 cut(s)	836, 1123, 1164, 1189
VspI	ATTAAT	1 cut(s)	810
XapI	RAATTY	5 cut(s)	330, 931, 1000, 1028, 2459
XbaI	TCTAGA	3 cut(s)	421, 1735, 2566
XceI	RCATGY	3 cut(s)	521, 1627, 2297
XhoI	CTCGAG	1 cut(s)	2378
XmiI	GTMKAC	2 cut(s)	159, 488
XspI	CTAG	8 cut(s)	422, 785, 861, 956, 1251, 1265, 1736, 2567
Zsp2I	ATGCAT	2 cut(s)	1720, 1840

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.