Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0004358

Physical Location & Seq

Genomic Coordinates Forward (+)

81558659 .. 81567104

Gene Structure 8446 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG575900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2253 bp

ATGTTTCTTTGTAGAATCCTACAAAGAGACGCTATAGAAGCTAACAGCGTTGATGAAGTTGTTAACTATGTTGAAGAATCTCTCACTTTGCGTCATACATCAACTAGGGAGTTGATGAAACTCTTGGAACATACAATTAATGTTGAGAGGGAAAAAACGGAGAACATAGCCCGTACTTTGAATGGAAAGATATCTTCAGAAGATGCTATTATTGAATTGTCCAAGATTGATGATATGATTGAAAAGGAGGCCAACAATCTACGTCAAGTGATTGATATCCTTCATTTGAAGCATAAAGAGTATGCTGATGTCATTCACACTCGCGCTAGTAGCGATTCAACAGATCAGTCTGAAATTAGACGAATTACAGGTGATCTCGATGACAGCATGGCTGAACTAGAGGAGAGTAGGAGAAAACTAGTCAATCTAAAAATGCAAAAGGATGTTGCATCTGGGATGCATAACCTTACTTCTGGTGCCGTGAATGGGACTTTGTCACCTGAAAAATCAACAGAAAGGACAATAAGCTTGCAGGAACTTAAAACTTCAATTGAGGAAACAAAGATACTTGCAGCCAGCCGCCTCTCTGAATTTCAAGAAGCACAGAAAGAAAATCTAGCATTGTCAAAACAATTGCAAGACTTTCAGAATGAAGTGAAGGATGAAAAATATGTACACTCATCTAGACTGTACACTATGCTAAATGATCAACTCCAGCATTGGAATGCTGAAGTGGAGAGATACAAATCATTGACTGGTTCTTTACAGGCTGACAGGGCTGTTGTCATGAGAAGGGAGAAGGAGTTAAATCTCAAACTAGAGTCAGCAGAGGCATTCAGGACCATAAATGAAAATGACTTTAGGATTGAGGAACTAGAGCTCCAGCTGCAGAAGTGCTTAATTGAAAAAAATGACTTAGAGATAAGCATGGAGGCAGCTGTTCAGGATTCAGGAAGAAAGGACATTACATCAGAGTTCCATGTGATGTCCTCATCTTTGTCGAAAGAGATGGAAATGATGGAAACTCAGTTGAAGCAGTGGAAGGAAACGGCTCACGGAACACTTTCCTTGCGTGAGAAATCCCAGTCTTTAAAAGCTTCATTAAGTACGAAGACAAATGAGCGGAACAGCTTAGCAAACAAATGTGCTGAACAGATGACAGAGATCAAGTCTCTCAAGGAATTGATTGAGAAGTTGCAGAAGGAGAAACTGGAACTTCAGATCTTCTTGGACTTGTACGCTCAGGAAAGTTACGTAAACAGAGACTTGTTGGAAATCAAAGAATCAGAACGCAGAGCTCACTCCCAAGCCGCTATGTTCAAAAATGCATTAGATGAACATAGTCTAGAGTTGAGGGTAAAAGCAGCCAATGAAGCTGAAGCTGCTTGTCAGCAAAGGCTTGCAGCCACTGAAGCTGAAATAACTGAATCAAGGGCCAAACTTGATGCATCTGAGAGGGATGTTCTGGAGCTTACCGAAGCTATTAAAATTAAAGACAAGGAGGCAGAGGCTTATATATCTGAAATTGAGACCATTGGTCAGGCATATGAAGATATGCAGACACAAAACCAGCATCTGTTGCAGCAGGTGACTGAGAGGGATGAATATAATATCAAGCTTGTTTCTGAGAGTGTGAAGACAAAGCAAGCACAGAACTTTCTACTTTCAGAGAAGCAGGCATTGGCAAAACAACTTCAACAAGTAAATACATCAGTAGAATCTCTGAAAATGAGAATTTCTCAAGATGAAGAGCAGATGAAGGCTGTCTTAACAGAAGCCTTGAAATCTACCGAGGAAGACAGACACCTTTCAGTCAATCTAGAAGCTGGAAAATGGGAACTGGCTGATTCTGAGAAGGAGTTGCAGTGGCTTAAATATGCTGTTGCTTCTTCTGAAAAGGAATATGCACGTATTAAACAGGATATTGAGGATATCCAATTGGAATTAGACAATGAGAGGAGCTTGAGGAAGAATATTGAGGAAGAACTATGGGAATTAAACAGCAGGGTTGTTGAGATGAGTTCTGAAACTGGAGAGGCTGCAATACAGAAACTCCAATCAGAAATCAAATTCTATAAGAATATTTTGCAGTGTAGTGTTTGTACTGACAGACCGAAGGAGGTGGTAATTGTGAAGTGCTTTCATCTATTTTGCAATTATTGCGTTCAAAAGAATTTAGAGATCCGTCACCGAAAGTGTCCTGCTTGTGGAACTCCGTTCGGTCAGAGTGACATACGCTTTGTAAAGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0002376 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006464 GO:0006513 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009791 GO:0009965 GO:0009987 GO:0010016 GO:0010154 GO:0010162 GO:0010228 GO:0010390 GO:0010431 GO:0016043 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0019538 GO:0021700 GO:0022414 GO:0022611 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0033523 GO:0036211 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045087 GO:0046983 GO:0048316 GO:0048366 GO:0048367 GO:0048608 GO:0048609 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051276 GO:0061458 GO:0070647 GO:0071695 GO:0071704 GO:0071840 GO:0099402 GO:1901564 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_3

Protein Analysis

750

Amino Acids

86.41

Weight (kDa)

5.17

Isoelectric Point (pI)

58.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_3	PF13920	696 - 740	3.7e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2	PF13923	697 - 736	6.4e-06	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	698 - 736	1.9e-07	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004358)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55250 AT1G55250 AT1G55250 AT1G55250 AT1G55250
fragaria_vesca	FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811
malus_domestica	MD08G1205100.v1.1 MD15G1388900.v1.1
prunus_persica	Prupe.1G537100_v2.0.a1
pyrus_communis	pycom08g17730 pycom15g34870
rosa_chinensis	RchiOBHm_Chr5g0081511
rosa_laevigata	RLG00000036943
rosa_multiflora	Rmu_sc0007702.1_g000003
rosa_roxburghii	Rroxscaffold_1G00002040
rosa_rugosa	Rorug05G0476800
rosa_samantha	Rh5AG528300 Rh5BG553000 Rh5CG575900 Rh5DG563100
rosa_wichuraiana	Rw5G049140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1576
Acc36I	ACCTGC	1 cut(s)	1576
AccB1I	GGYRCC	1 cut(s)	476
AccBSI	CCGCTC	1 cut(s)	1123
AccII	CGCG	1 cut(s)	324
AciI	CCGC	3 cut(s)	580, 1123, 1311
AclWI	GGATC	1 cut(s)	2176
AcsI	RAATTY	4 cut(s)	590, 1734, 2069, 2173
AcuI	CTGAAG	5 cut(s)	180, 750, 1202, 1398, 1431
AfaI	GTAC	6 cut(s)	175, 675, 692, 1108, 1238, 2104
AfiI	CCNNNNNNNGG	2 cut(s)	486, 2207
AhdI	GACNNNNNGTC	1 cut(s)	1536
AhlI	ACTAGT	1 cut(s)	418
AjuI	GAANNNNNNNTTGG	2 cut(s)	1664, 1696
AloI	GAACNNNNNNTCC	2 cut(s)	864, 896
Alw21I	GWGCWC	2 cut(s)	882, 1300
Alw26I	GTCTC	4 cut(s)	21, 1176, 1257, 1523
AlwI	GGATC	1 cut(s)	2176
AlwNI	CAGNNNCTG	2 cut(s)	1409, 1576
AoxI	GGCC	2 cut(s)	249, 1434
ApeKI	GCWGC	8 cut(s)	572, 886, 935, 1364, 1382, 1403, 1582, 2039
ApoI	RAATTY	4 cut(s)	590, 1734, 2069, 2173
ArsI	GACNNNNNNTTYG	1 cut(s)	2222
AseI	ATTAAT	1 cut(s)	138
Asp700I	GAANNNNTTC	1 cut(s)	1063
AspLEI	GCGC	1 cut(s)	326
AspS9I	GGNCC	2 cut(s)	840, 1434
AsuHPI	GGTGA	4 cut(s)	383, 489, 1600, 2180
AvaII	GGWCC	1 cut(s)	840
BanI	GGYRCC	1 cut(s)	476
BanII	GRGCYC	2 cut(s)	882, 1300
BbsI	GAAGAC	3 cut(s)	1118, 1643, 1803
Bbv12I	GWGCWC	2 cut(s)	882, 1300
BbvI	GCAGC	8 cut(s)	584, 873, 947, 1369, 1376, 1415, 1594, 2026
BccI	CCATC	2 cut(s)	1003, 1012
BceAI	ACGGC	2 cut(s)	464, 1065
BclI	TGATCA	1 cut(s)	706
BcoDI	GTCTC	4 cut(s)	21, 1176, 1257, 1523
BcuI	ACTAGT	1 cut(s)	418
BfmI	CTRYAG	2 cut(s)	33, 887
BfuAI	ACCTGC	1 cut(s)	1576
BglII	AGATCT	1 cut(s)	1221
BlpI	GCTNAGC	1 cut(s)	1132
Bme18I	GGWCC	1 cut(s)	840
BmeRI	GACNNNNNGTC	1 cut(s)	1536
BmgT120I	GGNCC	2 cut(s)	840, 1434
BmiI	GGNNCC	1 cut(s)	478
BmrI	ACTGGG	1 cut(s)	1078
BmsI	GCATC	6 cut(s)	193, 447, 458, 1435, 1457, 1582
BmuI	ACTGGG	1 cut(s)	1078
BpiI	GAAGAC	3 cut(s)	1118, 1643, 1803
BplI	GAGNNNNNCTC	2 cut(s)	1723, 1755
BpmI	CTGGAG	4 cut(s)	698, 866, 1487, 2052
Bpu10I	CCTNAGC	1 cut(s)	1242
Bpu1102I	GCTNAGC	1 cut(s)	1132
BpuEI	CTTGAG	3 cut(s)	1160, 1725, 1984
BsaAI	YACGTR	2 cut(s)	1255, 1910
BsaBI	GATNNNNATC	2 cut(s)	275, 745
BsaI	GGTCTC	1 cut(s)	1523
BsaJI	CCNNGG	1 cut(s)	1791
BsaXI	ACNNNNNCTCC	8 cut(s)	788, 818, 864, 894, 1850, 1880, 2037, 2067
Bsc4I	CCNNNNNNNGG	2 cut(s)	486, 2207
Bse1I	ACTGG	5 cut(s)	760, 1084, 1215, 1845, 2035
Bse8I	GATNNNNATC	2 cut(s)	275, 745
BseDI	CCNNGG	1 cut(s)	1791
BseGI	GGATG	5 cut(s)	448, 462, 667, 1465, 1606
BseJI	GATNNNNATC	2 cut(s)	275, 745
BseLI	CCNNNNNNNGG	2 cut(s)	486, 2207
BseMII	CTCAG	6 cut(s)	1040, 1256, 1443, 1584, 1617, 1842
BseNI	ACTGG	5 cut(s)	760, 1084, 1215, 1845, 2035
BseRI	GAGGAG	2 cut(s)	416, 1972
BseXI	GCAGC	8 cut(s)	584, 873, 947, 1369, 1376, 1415, 1594, 2026
Bsh1236I	CGCG	1 cut(s)	324
BshFI	GGCC	2 cut(s)	251, 1436
BshNI	GGYRCC	1 cut(s)	476
BsiHKAI	GWGCWC	2 cut(s)	882, 1300
BslFI	GGGAC	1 cut(s)	502
BslI	CCNNNNNNNGG	2 cut(s)	486, 2207
BsmAI	GTCTC	4 cut(s)	21, 1176, 1257, 1523
BsmBI	CGTCTC	1 cut(s)	21
BsmFI	GGGAC	1 cut(s)	502
BsmI	GAATGC	2 cut(s)	730, 833
BsnI	GGCC	2 cut(s)	251, 1436
Bso31I	GGTCTC	1 cut(s)	1523
Bsp1286I	GDGCHC	2 cut(s)	882, 1300
Bsp1407I	TGTACA	2 cut(s)	673, 690
Bsp143I	GATC	6 cut(s)	343, 373, 706, 1164, 1221, 2181
Bsp1720I	GCTNAGC	1 cut(s)	1132
BspACI	CCGC	3 cut(s)	580, 1123, 1311
BspANI	GGCC	2 cut(s)	251, 1436
BspCNI	CTCAG	6 cut(s)	1039, 1255, 1444, 1585, 1618, 1843
BspFNI	CGCG	1 cut(s)	324
BspHI	TCATGA	1 cut(s)	786
BspLI	GGNNCC	1 cut(s)	478
BspMAI	CTGCAG	1 cut(s)	891
BspMI	ACCTGC	1 cut(s)	1576
BspPI	GGATC	1 cut(s)	2176
BspQI	GCTCTTC	1 cut(s)	1743
BspT107I	GGYRCC	1 cut(s)	476
BspTNI	GGTCTC	1 cut(s)	1523
BsrBI	CCGCTC	1 cut(s)	1123
BsrGI	TGTACA	2 cut(s)	673, 690
BsrI	ACTGG	5 cut(s)	760, 1084, 1215, 1845, 2035
BssECI	CCNNGG	1 cut(s)	1791
BssMI	GATC	6 cut(s)	343, 373, 706, 1164, 1221, 2181
Bst4CI	ACNGT	1 cut(s)	690
Bst6I	CTCTTC	1 cut(s)	1743
BstAPI	GCANNNNNTGC	2 cut(s)	1579, 2160
BstAUI	TGTACA	2 cut(s)	673, 690
BstBAI	YACGTR	2 cut(s)	1255, 1910
BstC8I	GCNNGC	5 cut(s)	530, 577, 1401, 1647, 1677
BstDEI	CTNAG	8 cut(s)	916, 1026, 1132, 1242, 1452, 1593, 1626, 1851
BstF5I	GGATG	5 cut(s)	448, 462, 667, 1465, 1606
BstFNI	CGCG	1 cut(s)	324
BstHHI	GCGC	1 cut(s)	326
BstKTI	GATC	6 cut(s)	346, 376, 709, 1167, 1224, 2184
BstMAI	GTCTC	4 cut(s)	21, 1176, 1257, 1523
BstMBI	GATC	6 cut(s)	343, 373, 706, 1164, 1221, 2181
BstMWI	GCNNNNNNNGC	9 cut(s)	38, 330, 776, 886, 1373, 1382, 1412, 1579, 2160
BstSFI	CTRYAG	2 cut(s)	33, 887
BstSNI	TACGTA	1 cut(s)	1255
BstUI	CGCG	1 cut(s)	324
BstV1I	GCAGC	8 cut(s)	584, 873, 947, 1369, 1376, 1415, 1594, 2026
BstV2I	GAAGAC	3 cut(s)	1118, 1643, 1803
BstX2I	RGATCY	2 cut(s)	1221, 2181
BstYI	RGATCY	2 cut(s)	1221, 2181
BsuRI	GGCC	2 cut(s)	251, 1436
BtsCI	GGATG	5 cut(s)	448, 462, 667, 1465, 1606
BtsI	GCAGTG	3 cut(s)	1043, 1871, 2096
BtsIMutI	CAGTG	4 cut(s)	1043, 1407, 1871, 2096
BveI	ACCTGC	1 cut(s)	1576
Cac8I	GCNNGC	5 cut(s)	530, 577, 1401, 1647, 1677
CaiI	CAGNNNCTG	2 cut(s)	1409, 1576
CciI	TCATGA	1 cut(s)	786
CfoI	GCGC	1 cut(s)	326
Cfr13I	GGNCC	2 cut(s)	840, 1434
CseI	GACGC	2 cut(s)	38, 80
Csp6I	GTAC	6 cut(s)	174, 674, 691, 1107, 1237, 2103
CviAII	CATG	4 cut(s)	388, 787, 928, 980
CviQI	GTAC	6 cut(s)	174, 674, 691, 1107, 1237, 2103
DdeI	CTNAG	8 cut(s)	916, 1026, 1132, 1242, 1452, 1593, 1626, 1851
DpnI	GATC	6 cut(s)	345, 375, 708, 1166, 1223, 2183
DpnII	GATC	6 cut(s)	343, 373, 706, 1164, 1221, 2181
DraI	TTTAAA	1 cut(s)	1092
DriI	GACNNNNNGTC	1 cut(s)	1536
Eam1104I	CTCTTC	1 cut(s)	1743
Eam1105I	GACNNNNNGTC	1 cut(s)	1536
EarI	CTCTTC	1 cut(s)	1743
Ecl136II	GAGCTC	2 cut(s)	880, 1298
Eco105I	TACGTA	1 cut(s)	1255
Eco24I	GRGCYC	2 cut(s)	882, 1300
Eco31I	GGTCTC	1 cut(s)	1523
Eco32I	GATATC	3 cut(s)	192, 277, 1933
Eco47I	GGWCC	1 cut(s)	840
Eco53kI	GAGCTC	2 cut(s)	880, 1298
Eco57I	CTGAAG	5 cut(s)	180, 750, 1202, 1398, 1431
EcoICRI	GAGCTC	2 cut(s)	880, 1298
EcoRV	GATATC	3 cut(s)	192, 277, 1933
EcoT22I	ATGCAT	3 cut(s)	462, 1330, 1450
EcoT38I	GRGCYC	2 cut(s)	882, 1300
Esp3I	CGTCTC	1 cut(s)	21
FaeI	CATG	4 cut(s)	391, 790, 931, 983
FalI	AAGNNNNNCTT	2 cut(s)	1751, 1783
FaqI	GGGAC	1 cut(s)	502
FatI	CATG	4 cut(s)	387, 786, 927, 979
FauNDI	CATATG	1 cut(s)	1546
FbaI	TGATCA	1 cut(s)	706
FokI	GGATG	5 cut(s)	455, 469, 674, 1472, 1613
FriOI	GRGCYC	2 cut(s)	882, 1300
GlaI	GCGC	1 cut(s)	325
GsuI	CTGGAG	4 cut(s)	698, 866, 1487, 2052
HaeIII	GGCC	2 cut(s)	251, 1436
HgaI	GACGC	2 cut(s)	38, 80
HhaI	GCGC	1 cut(s)	326
Hin1II	CATG	4 cut(s)	391, 790, 931, 983
Hin6I	GCGC	1 cut(s)	324
HinP1I	GCGC	1 cut(s)	324
HincII	GTYRAC	1 cut(s)	64
HindII	GTYRAC	1 cut(s)	64
HindIII	AAGCTT	3 cut(s)	526, 1095, 1616
HinfI	GANTC	9 cut(s)	15, 77, 335, 821, 947, 1283, 1427, 1718, 1847
HpaI	GTTAAC	1 cut(s)	64
HphI	GGTGA	4 cut(s)	383, 489, 1600, 2180
Hpy166II	GTNNAC	4 cut(s)	64, 676, 693, 1258
Hpy8I	GTNNAC	4 cut(s)	64, 676, 693, 1258
HpyAV	CCTTC	9 cut(s)	290, 652, 786, 793, 1036, 1195, 1753, 1849, 2110
HpyCH4III	ACNGT	1 cut(s)	690
HpyCH4IV	ACGT	3 cut(s)	262, 1254, 1909
HpyF10VI	GCNNNNNNNGC	9 cut(s)	38, 330, 776, 886, 1373, 1382, 1412, 1579, 2160
HpyF3I	CTNAG	8 cut(s)	916, 1026, 1132, 1242, 1452, 1593, 1626, 1851
HpySE526I	ACGT	3 cut(s)	262, 1254, 1909
Hsp92II	CATG	4 cut(s)	391, 790, 931, 983
HspAI	GCGC	1 cut(s)	324
Ksp22I	TGATCA	1 cut(s)	706
KspAI	GTTAAC	1 cut(s)	64
Kzo9I	GATC	6 cut(s)	343, 373, 706, 1164, 1221, 2181
LguI	GCTCTTC	1 cut(s)	1743
LmnI	GCTCC	3 cut(s)	885, 1468, 1959
Lsp1109I	GCAGC	8 cut(s)	584, 873, 947, 1369, 1376, 1415, 1594, 2026
LweI	GCATC	6 cut(s)	193, 447, 458, 1435, 1457, 1582
MaeII	ACGT	3 cut(s)	262, 1254, 1909
MaeIII	GTNAC	5 cut(s)	495, 1250, 1588, 2186, 2228
MalI	GATC	6 cut(s)	345, 375, 708, 1166, 1223, 2183
MbiI	CCGCTC	1 cut(s)	1123
MboI	GATC	6 cut(s)	343, 373, 706, 1164, 1221, 2181
MfeI	CAATTG	3 cut(s)	549, 632, 1937
MflI	RGATCY	2 cut(s)	1221, 2181
MhlI	GDGCHC	2 cut(s)	882, 1300
MlyI	GAGTC	1 cut(s)	830
MmeI	TCCRAC	1 cut(s)	1251
Mph1103I	ATGCAT	3 cut(s)	462, 1330, 1450
MroXI	GAANNNNTTC	1 cut(s)	1063
MslI	CAYNNNNRTG	1 cut(s)	723
MspA1I	CMGCKG	2 cut(s)	886, 938
MunI	CAATTG	3 cut(s)	549, 632, 1937
Mva1269I	GAATGC	2 cut(s)	730, 833
MvnI	CGCG	1 cut(s)	324
MwoI	GCNNNNNNNGC	9 cut(s)	38, 330, 776, 886, 1373, 1382, 1412, 1579, 2160
NdeI	CATATG	1 cut(s)	1546
NdeII	GATC	6 cut(s)	343, 373, 706, 1164, 1221, 2181
NlaIII	CATG	4 cut(s)	391, 790, 931, 983
NlaIV	GGNNCC	1 cut(s)	478
NmuCI	GTSAC	4 cut(s)	495, 1588, 2186, 2228
NsiI	ATGCAT	3 cut(s)	462, 1330, 1450
PagI	TCATGA	1 cut(s)	786
PaqCI	CACCTGC	1 cut(s)	1576
PciSI	GCTCTTC	1 cut(s)	1743
PctI	GAATGC	2 cut(s)	730, 833
PdmI	GAANNNNTTC	1 cut(s)	1063
PfeI	GAWTC	8 cut(s)	15, 77, 335, 947, 1283, 1427, 1718, 1847
PflFI	GACNNNGTC	1 cut(s)	493
PleI	GAGTC	1 cut(s)	829
PpsI	GAGTC	1 cut(s)	829
Ppu21I	YACGTR	2 cut(s)	1255, 1910
PshBI	ATTAAT	1 cut(s)	138
Psp124BI	GAGCTC	2 cut(s)	882, 1300
PspN4I	GGNNCC	1 cut(s)	478
PspPI	GGNCC	2 cut(s)	840, 1434
PstI	CTGCAG	1 cut(s)	891
PstNI	CAGNNNCTG	2 cut(s)	1409, 1576
PsuI	RGATCY	2 cut(s)	1221, 2181
PsyI	GACNNNGTC	1 cut(s)	493
PvuII	CAGCTG	2 cut(s)	886, 938
RsaI	GTAC	6 cut(s)	175, 675, 692, 1108, 1238, 2104
RsaNI	GTAC	6 cut(s)	174, 674, 691, 1107, 1237, 2103
RseI	CAYNNNNRTG	1 cut(s)	723
SacI	GAGCTC	2 cut(s)	882, 1300
SapI	GCTCTTC	1 cut(s)	1743
Sau3AI	GATC	6 cut(s)	343, 373, 706, 1164, 1221, 2181
Sau96I	GGNCC	2 cut(s)	840, 1434
SchI	GAGTC	1 cut(s)	830
SduI	GDGCHC	2 cut(s)	882, 1300
SfaNI	GCATC	6 cut(s)	193, 447, 458, 1435, 1457, 1582
SfcI	CTRYAG	2 cut(s)	33, 887
SinI	GGWCC	1 cut(s)	840
SmiMI	CAYNNNNRTG	1 cut(s)	723
SmlI	CTYRAG	3 cut(s)	1175, 1740, 1963
SmoI	CTYRAG	3 cut(s)	1175, 1740, 1963
SnaBI	TACGTA	1 cut(s)	1255
SpeI	ACTAGT	1 cut(s)	418
SsiI	CCGC	3 cut(s)	580, 1123, 1311
SspI	AATATT	2 cut(s)	1975, 2083
SstI	GAGCTC	2 cut(s)	882, 1300
TaaI	ACNGT	1 cut(s)	690
TaiI	ACGT	3 cut(s)	265, 1257, 1912
TaqI	TCGA	2 cut(s)	378, 1001
TaqII	GACCGA	2 cut(s)	2128, 2210
TatI	WGTACW	3 cut(s)	673, 690, 2102
TauI	GCSGC	2 cut(s)	582, 1313
TfiI	GAWTC	8 cut(s)	15, 77, 335, 947, 1283, 1427, 1718, 1847
TscAI	CASTG	4 cut(s)	1043, 1414, 1871, 2096
TseFI	GTSAC	4 cut(s)	495, 1588, 2186, 2228
TseI	GCWGC	8 cut(s)	572, 886, 935, 1364, 1382, 1403, 1582, 2039
Tsp45I	GTSAC	4 cut(s)	495, 1588, 2186, 2228
TspGWI	ACGGA	4 cut(s)	173, 1071, 2174, 2205
TspRI	CASTG	4 cut(s)	1043, 1414, 1871, 2096
Tth111I	GACNNNGTC	1 cut(s)	493
VpaK11BI	GGWCC	1 cut(s)	840
VspI	ATTAAT	1 cut(s)	138
XapI	RAATTY	4 cut(s)	590, 1734, 2069, 2173
XbaI	TCTAGA	3 cut(s)	683, 1345, 1819
XmnI	GAANNNNTTC	1 cut(s)	1063
Zsp2I	ATGCAT	3 cut(s)	462, 1330, 1450

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.