Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0004358

Physical Location & Seq

Genomic Coordinates Forward (+)

87851907 .. 87859766

Gene Structure 7860 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG553000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2499 bp

ATGATGCTTCAGCAGATAGACAAACAGAAGCATGAATTGCAAGATCTTGAAGCAAACATCAAAGAACTGAAGGCGAAGCAAGGTTCTTACGATGACATGCTAATTGCGGTGAATCAGCTGTGGAATCAGTTAGTTGGCGATGTAGCCCTTCTTGGAGTATGTGCTGGAGCAGGCCAAAATGCGTTGCAAGTTTTGGATTCTGCTGATTATTCTCGCGGTTCAATTCCGTCATGCCCTGCGGAGGAGATGTTTCTTTGTAGAATCCTACAAAGAGACGCTATAGAAGCTAACAGCGTTGATGAAGTTGTTAACTATGTTGAAGAATCTCTCACTTTGCGTCATACATCAACTAGGGAGTTGATGAAACTCTTGGAACATACAATTAATGTTGAGAGGGAAAAAACGGAGAACATAGCCCGTACTTTGAATGGAAAGATATCTTCAGAAGATGCTATTATTGAATTGTCCAAGATTGATGATATGATTGAAAAGGAGGCCAACAATCTACGTCAAGTGATTGATATCCTTCATTTGAAGCATAAAGAGTATGCTGATGTCATTCACACTCGCGCTAGTAGCGATTCAACAGATCAGTCTGAAATTAGACGAATTACAGGTGATCTCGATGACAGCATGGCTGAACTAGAGGAGAGTAGGAGAAAACTAGTCAATCTAAAAATGCAAAAGGATGTTGCATCTGGGATGCATAACCTTACTTCTGGTGCCGTGAATGGGACTTTGTCACCTGAAAAATCAACAGAAAGGACAATAAGCTTGCAGGAACTTAAAACTTCAATTGAGGAAACAAAGATACTTGCAGCCAGCCGCCTCTCTGAATTTCAAGAAGCACAGAAAGAAAATCTAGCATTGTCAAAACAATTGCAAGACTTTCAGAATGAAGTGAAGGATGAAAAATATGTACACTCATCTAGACTGTACACTATGCTAAATGATCAACTCCAGCATTGGAATGCTGAAGTGGAGAGATACAAATCATTGACTGGTTCTTTACAGGCTGACAGGGCTGTTGTCATGAGAAGGGAGAAGGAGTTAAATCTCAAACTAGAGTCAGCAGAGGCATTCAGGACCATAAATGAAAATGACTTTAGGATTGAGGAACTAGAGCTCCAGCTGCAGAAGTGCTTAATTGAAAAAAATGACTTAGAGATAAGCATGGAGGCAGCTGTTCAGGATTCAGGAAGAAAGGACATTACATCAGAGTTCCATGTGATGTCCTCATCTTTGTCGAAAGAGATGGAAATGATGGAAACTCAGTTGAAGCAGTGGAAGGAAACGGCTCACGGAACACTTTCCTTGCGTGAGAAATCCCAGTCTTTAAAAGCTTCATTAAGTACGAAGACAAATGAGCGGAACAGCTTAGCAAACAAATGTGCTGAACAGATGACAGAGATCAAGTCTCTCAAGGAATTGATTGAGAAGTTGCAGAAGGAGAAACTGGAACTTCAGATCTTCTTGGACTTGTACGCTCAGGAAAGTTACGTAAACAGAGACTTGTTGGAAATCAAAGAATCAGAACGCAGAGCTCACTCCCAAGCCGCTATGTTCAAAAATGCATTAGATGAACATAGTCTAGAGTTGAGGGTAAAAGCAGCCAATGAAGCTGAAGCTGCTTGTCAGCAAAGGCTTGCAGCCACTGAAGCTGAAATAACTGAATCAAGGGCCAAACTTGATGCATCTGAGAGGGATGTTCTGGAGCTTACCGAAGCTATTAAAATTAAAGACAAGGAGGCAGAGGCTTATATATCTGAAATTGAGACCATTGGTCAGGCATATGAAGATATGCAGACACAAAACCAGCATCTGTTGCAGCAGGTGACTGAGAGGGATGAATATAATATCAAGCTTGTTTCTGAGAGTGTGAAGACAAAGCAAGCACAGAACTTTCTACTTTCAGAGAAGCAGGCATTGGCAAAACAACTTCAACAAGTAAATACATCAGTAGAATCTCTGAAAATGAGAATTTCTCAAGATGAAGAGCAGATGAAGGCTGTCTTAACAGAAGCCTTGAAATCTACCGAGGAAGACAGACACCTTTCAGTCAATCTAGAAGCTGGAAAATGGGAACTGGCTGATTCTGAGAAGGAGTTGCAGTGGCTTAAATATGCTGTTGCTTCTTCTGAAAAGGAATATGCACGTATTAAACAGGATATTGAGGATATCCAATTGGAATTAGACAATGAGAGGAGCTTGAGGAAGAATATTGAGGAAGAACTATGGGAATTAAACAGCAGGGTTGTTGAGATGAGTTCTGAAACTGGAGAGGCTGCAATACAGAAACTCCAATCAGAAATCAAATTCTATAAGAATATTTTGCAGTGTAGTGTTTGTACTGACAGACCGAAGGAGGTGGTAATTGTGAAGTGCTTTCATCTATTTTGCAATTATTGCGTTCAAAAGAATTTAGAGATCCGTCACCGAAAGTGTCCTGCTTGTGGAACTCCGTTCGGTCAGAGTGACATACGCTTTGTAAAGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0002376 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006464 GO:0006513 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009791 GO:0009965 GO:0009987 GO:0010016 GO:0010154 GO:0010162 GO:0010228 GO:0010390 GO:0010431 GO:0016043 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0019538 GO:0021700 GO:0022414 GO:0022611 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0033523 GO:0036211 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045087 GO:0046983 GO:0048316 GO:0048366 GO:0048367 GO:0048608 GO:0048609 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051276 GO:0061458 GO:0070647 GO:0071695 GO:0071704 GO:0071840 GO:0099402 GO:1901564 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_3

Protein Analysis

832

Amino Acids

95.32

Weight (kDa)

5.08

Isoelectric Point (pI)

57.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_3	PF13920	778 - 822	4.2e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2	PF13923	779 - 818	7.3e-06	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	780 - 818	2.1e-07	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004358)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55250 AT1G55250 AT1G55250 AT1G55250 AT1G55250
fragaria_vesca	FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811 FvH4_3g44811
malus_domestica	MD08G1205100.v1.1 MD15G1388900.v1.1
prunus_persica	Prupe.1G537100_v2.0.a1
pyrus_communis	pycom08g17730 pycom15g34870
rosa_chinensis	RchiOBHm_Chr5g0081511
rosa_laevigata	RLG00000036943
rosa_multiflora	Rmu_sc0007702.1_g000003
rosa_roxburghii	Rroxscaffold_1G00002040
rosa_rugosa	Rorug05G0476800
rosa_samantha	Rh5AG528300 Rh5BG553000 Rh5CG575900 Rh5DG563100
rosa_wichuraiana	Rw5G049140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1822
Acc36I	ACCTGC	1 cut(s)	1822
AccB1I	GGYRCC	1 cut(s)	722
AccBSI	CCGCTC	1 cut(s)	1369
AccII	CGCG	2 cut(s)	216, 570
AciI	CCGC	6 cut(s)	107, 216, 239, 826, 1369, 1557
AclWI	GGATC	1 cut(s)	2422
AcsI	RAATTY	4 cut(s)	836, 1980, 2315, 2419
AcuI	CTGAAG	6 cut(s)	89, 426, 996, 1448, 1644, 1677
AfaI	GTAC	6 cut(s)	421, 921, 938, 1354, 1484, 2350
AfiI	CCNNNNNNNGG	3 cut(s)	241, 732, 2453
AhdI	GACNNNNNGTC	1 cut(s)	1782
AhlI	ACTAGT	1 cut(s)	664
AjuI	GAANNNNNNNTTGG	2 cut(s)	1910, 1942
AloI	GAACNNNNNNTCC	2 cut(s)	1110, 1142
Alw21I	GWGCWC	2 cut(s)	1128, 1546
Alw26I	GTCTC	4 cut(s)	267, 1422, 1503, 1769
AlwI	GGATC	1 cut(s)	2422
AlwNI	CAGNNNCTG	2 cut(s)	1655, 1822
AoxI	GGCC	3 cut(s)	172, 495, 1680
ApeKI	GCWGC	8 cut(s)	818, 1132, 1181, 1610, 1628, 1649, 1828, 2285
ApoI	RAATTY	4 cut(s)	836, 1980, 2315, 2419
ArsI	GACNNNNNNTTYG	1 cut(s)	2468
AseI	ATTAAT	1 cut(s)	384
Asp700I	GAANNNNTTC	1 cut(s)	1309
AspLEI	GCGC	1 cut(s)	572
AspS9I	GGNCC	2 cut(s)	1086, 1680
AsuHPI	GGTGA	5 cut(s)	121, 629, 735, 1846, 2426
AvaII	GGWCC	1 cut(s)	1086
BanI	GGYRCC	1 cut(s)	722
BanII	GRGCYC	2 cut(s)	1128, 1546
BbsI	GAAGAC	3 cut(s)	1364, 1889, 2049
Bbv12I	GWGCWC	2 cut(s)	1128, 1546
BbvI	GCAGC	8 cut(s)	830, 1119, 1193, 1615, 1622, 1661, 1840, 2272
BccI	CCATC	2 cut(s)	1249, 1258
BceAI	ACGGC	2 cut(s)	710, 1311
BclI	TGATCA	1 cut(s)	952
BcoDI	GTCTC	4 cut(s)	267, 1422, 1503, 1769
BcuI	ACTAGT	1 cut(s)	664
BfmI	CTRYAG	2 cut(s)	279, 1133
BfuAI	ACCTGC	1 cut(s)	1822
BglII	AGATCT	2 cut(s)	43, 1467
BlpI	GCTNAGC	1 cut(s)	1378
Bme18I	GGWCC	1 cut(s)	1086
BmeRI	GACNNNNNGTC	1 cut(s)	1782
BmgT120I	GGNCC	2 cut(s)	1086, 1680
BmiI	GGNNCC	1 cut(s)	724
BmrI	ACTGGG	1 cut(s)	1324
BmsI	GCATC	6 cut(s)	439, 693, 704, 1681, 1703, 1828
BmuI	ACTGGG	1 cut(s)	1324
BpiI	GAAGAC	3 cut(s)	1364, 1889, 2049
BplI	GAGNNNNNCTC	2 cut(s)	1969, 2001
BpmI	CTGGAG	5 cut(s)	186, 944, 1112, 1733, 2298
Bpu10I	CCTNAGC	1 cut(s)	1488
Bpu1102I	GCTNAGC	1 cut(s)	1378
BpuEI	CTTGAG	3 cut(s)	1406, 1971, 2230
BsaAI	YACGTR	2 cut(s)	1501, 2156
BsaBI	GATNNNNATC	2 cut(s)	521, 991
BsaI	GGTCTC	1 cut(s)	1769
BsaJI	CCNNGG	1 cut(s)	2037
Bsc4I	CCNNNNNNNGG	3 cut(s)	241, 732, 2453
Bse1I	ACTGG	5 cut(s)	1006, 1330, 1461, 2091, 2281
Bse8I	GATNNNNATC	2 cut(s)	521, 991
BseDI	CCNNGG	1 cut(s)	2037
BseGI	GGATG	5 cut(s)	694, 708, 913, 1711, 1852
BseJI	GATNNNNATC	2 cut(s)	521, 991
BseLI	CCNNNNNNNGG	3 cut(s)	241, 732, 2453
BseMII	CTCAG	6 cut(s)	1286, 1502, 1689, 1830, 1863, 2088
BseNI	ACTGG	5 cut(s)	1006, 1330, 1461, 2091, 2281
BseRI	GAGGAG	3 cut(s)	257, 662, 2218
BseXI	GCAGC	8 cut(s)	830, 1119, 1193, 1615, 1622, 1661, 1840, 2272
Bsh1236I	CGCG	2 cut(s)	216, 570
BshFI	GGCC	3 cut(s)	174, 497, 1682
BshNI	GGYRCC	1 cut(s)	722
BsiHKAI	GWGCWC	2 cut(s)	1128, 1546
BslFI	GGGAC	1 cut(s)	748
BslI	CCNNNNNNNGG	3 cut(s)	241, 732, 2453
BsmAI	GTCTC	4 cut(s)	267, 1422, 1503, 1769
BsmBI	CGTCTC	1 cut(s)	267
BsmFI	GGGAC	1 cut(s)	748
BsmI	GAATGC	2 cut(s)	976, 1079
BsnI	GGCC	3 cut(s)	174, 497, 1682
Bso31I	GGTCTC	1 cut(s)	1769
Bsp1286I	GDGCHC	2 cut(s)	1128, 1546
Bsp1407I	TGTACA	2 cut(s)	919, 936
Bsp143I	GATC	7 cut(s)	43, 589, 619, 952, 1410, 1467, 2427
Bsp1720I	GCTNAGC	1 cut(s)	1378
BspACI	CCGC	6 cut(s)	107, 216, 239, 826, 1369, 1557
BspANI	GGCC	3 cut(s)	174, 497, 1682
BspCNI	CTCAG	6 cut(s)	1285, 1501, 1690, 1831, 1864, 2089
BspFNI	CGCG	2 cut(s)	216, 570
BspHI	TCATGA	1 cut(s)	1032
BspLI	GGNNCC	1 cut(s)	724
BspMAI	CTGCAG	1 cut(s)	1137
BspMI	ACCTGC	1 cut(s)	1822
BspPI	GGATC	1 cut(s)	2422
BspQI	GCTCTTC	1 cut(s)	1989
BspT107I	GGYRCC	1 cut(s)	722
BspTNI	GGTCTC	1 cut(s)	1769
BsrBI	CCGCTC	1 cut(s)	1369
BsrGI	TGTACA	2 cut(s)	919, 936
BsrI	ACTGG	5 cut(s)	1006, 1330, 1461, 2091, 2281
BssECI	CCNNGG	1 cut(s)	2037
BssMI	GATC	7 cut(s)	43, 589, 619, 952, 1410, 1467, 2427
Bst4CI	ACNGT	1 cut(s)	936
Bst6I	CTCTTC	1 cut(s)	1989
BstAPI	GCANNNNNTGC	3 cut(s)	37, 1825, 2406
BstAUI	TGTACA	2 cut(s)	919, 936
BstBAI	YACGTR	2 cut(s)	1501, 2156
BstC8I	GCNNGC	6 cut(s)	172, 776, 823, 1647, 1893, 1923
BstDEI	CTNAG	8 cut(s)	1162, 1272, 1378, 1488, 1698, 1839, 1872, 2097
BstF5I	GGATG	5 cut(s)	694, 708, 913, 1711, 1852
BstFNI	CGCG	2 cut(s)	216, 570
BstHHI	GCGC	1 cut(s)	572
BstKTI	GATC	7 cut(s)	46, 592, 622, 955, 1413, 1470, 2430
BstMAI	GTCTC	4 cut(s)	267, 1422, 1503, 1769
BstMBI	GATC	7 cut(s)	43, 589, 619, 952, 1410, 1467, 2427
BstNSI	RCATGY	1 cut(s)	100
BstSFI	CTRYAG	2 cut(s)	279, 1133
BstSNI	TACGTA	1 cut(s)	1501
BstUI	CGCG	2 cut(s)	216, 570
BstV1I	GCAGC	8 cut(s)	830, 1119, 1193, 1615, 1622, 1661, 1840, 2272
BstV2I	GAAGAC	3 cut(s)	1364, 1889, 2049
BstX2I	RGATCY	3 cut(s)	43, 1467, 2427
BstYI	RGATCY	3 cut(s)	43, 1467, 2427
BsuRI	GGCC	3 cut(s)	174, 497, 1682
BtgZI	GCGATG	1 cut(s)	153
BtsCI	GGATG	5 cut(s)	694, 708, 913, 1711, 1852
BtsI	GCAGTG	3 cut(s)	1289, 2117, 2342
BtsIMutI	CAGTG	4 cut(s)	1289, 1653, 2117, 2342
BveI	ACCTGC	1 cut(s)	1822
Cac8I	GCNNGC	6 cut(s)	172, 776, 823, 1647, 1893, 1923
CaiI	CAGNNNCTG	2 cut(s)	1655, 1822
CciI	TCATGA	1 cut(s)	1032
CfoI	GCGC	1 cut(s)	572
Cfr13I	GGNCC	2 cut(s)	1086, 1680
CseI	GACGC	2 cut(s)	284, 326
Csp6I	GTAC	6 cut(s)	420, 920, 937, 1353, 1483, 2349
CviAII	CATG	7 cut(s)	32, 97, 231, 634, 1033, 1174, 1226
CviQI	GTAC	6 cut(s)	420, 920, 937, 1353, 1483, 2349
DdeI	CTNAG	8 cut(s)	1162, 1272, 1378, 1488, 1698, 1839, 1872, 2097
DpnI	GATC	7 cut(s)	45, 591, 621, 954, 1412, 1469, 2429
DpnII	GATC	7 cut(s)	43, 589, 619, 952, 1410, 1467, 2427
DraI	TTTAAA	1 cut(s)	1338
DriI	GACNNNNNGTC	1 cut(s)	1782
Eam1104I	CTCTTC	1 cut(s)	1989
Eam1105I	GACNNNNNGTC	1 cut(s)	1782
EarI	CTCTTC	1 cut(s)	1989
Ecl136II	GAGCTC	2 cut(s)	1126, 1544
Eco105I	TACGTA	1 cut(s)	1501
Eco24I	GRGCYC	2 cut(s)	1128, 1546
Eco31I	GGTCTC	1 cut(s)	1769
Eco32I	GATATC	3 cut(s)	438, 523, 2179
Eco47I	GGWCC	1 cut(s)	1086
Eco53kI	GAGCTC	2 cut(s)	1126, 1544
Eco57I	CTGAAG	6 cut(s)	89, 426, 996, 1448, 1644, 1677
EcoICRI	GAGCTC	2 cut(s)	1126, 1544
EcoRV	GATATC	3 cut(s)	438, 523, 2179
EcoT22I	ATGCAT	3 cut(s)	708, 1576, 1696
EcoT38I	GRGCYC	2 cut(s)	1128, 1546
Esp3I	CGTCTC	1 cut(s)	267
FaeI	CATG	7 cut(s)	35, 100, 234, 637, 1036, 1177, 1229
FalI	AAGNNNNNCTT	2 cut(s)	1997, 2029
FaqI	GGGAC	1 cut(s)	748
FatI	CATG	7 cut(s)	31, 96, 230, 633, 1032, 1173, 1225
FauNDI	CATATG	1 cut(s)	1792
FbaI	TGATCA	1 cut(s)	952
FokI	GGATG	5 cut(s)	701, 715, 920, 1718, 1859
FriOI	GRGCYC	2 cut(s)	1128, 1546
GlaI	GCGC	1 cut(s)	571
GsuI	CTGGAG	5 cut(s)	186, 944, 1112, 1733, 2298
HaeIII	GGCC	3 cut(s)	174, 497, 1682
HgaI	GACGC	2 cut(s)	284, 326
HhaI	GCGC	1 cut(s)	572
Hin1II	CATG	7 cut(s)	35, 100, 234, 637, 1036, 1177, 1229
Hin6I	GCGC	1 cut(s)	570
HinP1I	GCGC	1 cut(s)	570
HincII	GTYRAC	1 cut(s)	310
HindII	GTYRAC	1 cut(s)	310
HindIII	AAGCTT	3 cut(s)	772, 1341, 1862
HpaI	GTTAAC	1 cut(s)	310
HphI	GGTGA	5 cut(s)	121, 629, 735, 1846, 2426
Hpy166II	GTNNAC	4 cut(s)	310, 922, 939, 1504
Hpy8I	GTNNAC	4 cut(s)	310, 922, 939, 1504
HpyCH4III	ACNGT	1 cut(s)	936
HpyCH4IV	ACGT	3 cut(s)	508, 1500, 2155
HpyF3I	CTNAG	8 cut(s)	1162, 1272, 1378, 1488, 1698, 1839, 1872, 2097
HpySE526I	ACGT	3 cut(s)	508, 1500, 2155
Hsp92II	CATG	7 cut(s)	35, 100, 234, 637, 1036, 1177, 1229
HspAI	GCGC	1 cut(s)	570
Ksp22I	TGATCA	1 cut(s)	952
KspAI	GTTAAC	1 cut(s)	310
Kzo9I	GATC	7 cut(s)	43, 589, 619, 952, 1410, 1467, 2427
LguI	GCTCTTC	1 cut(s)	1989
LmnI	GCTCC	4 cut(s)	167, 1131, 1714, 2205
Lsp1109I	GCAGC	8 cut(s)	830, 1119, 1193, 1615, 1622, 1661, 1840, 2272
LweI	GCATC	6 cut(s)	439, 693, 704, 1681, 1703, 1828
MaeII	ACGT	3 cut(s)	508, 1500, 2155
MaeIII	GTNAC	5 cut(s)	741, 1496, 1834, 2432, 2474
MalI	GATC	7 cut(s)	45, 591, 621, 954, 1412, 1469, 2429
MbiI	CCGCTC	1 cut(s)	1369
MboI	GATC	7 cut(s)	43, 589, 619, 952, 1410, 1467, 2427
MfeI	CAATTG	3 cut(s)	795, 878, 2183
MflI	RGATCY	3 cut(s)	43, 1467, 2427
MhlI	GDGCHC	2 cut(s)	1128, 1546
MlyI	GAGTC	1 cut(s)	1076
MmeI	TCCRAC	1 cut(s)	1497
Mph1103I	ATGCAT	3 cut(s)	708, 1576, 1696
MroXI	GAANNNNTTC	1 cut(s)	1309
MslI	CAYNNNNRTG	1 cut(s)	969
MspA1I	CMGCKG	3 cut(s)	118, 1132, 1184
MunI	CAATTG	3 cut(s)	795, 878, 2183
Mva1269I	GAATGC	2 cut(s)	976, 1079
MvnI	CGCG	2 cut(s)	216, 570
NdeI	CATATG	1 cut(s)	1792
NdeII	GATC	7 cut(s)	43, 589, 619, 952, 1410, 1467, 2427
NlaIII	CATG	7 cut(s)	35, 100, 234, 637, 1036, 1177, 1229
NlaIV	GGNNCC	1 cut(s)	724
NmuCI	GTSAC	4 cut(s)	741, 1834, 2432, 2474
NsiI	ATGCAT	3 cut(s)	708, 1576, 1696
NspI	RCATGY	1 cut(s)	100
PagI	TCATGA	1 cut(s)	1032
PaqCI	CACCTGC	1 cut(s)	1822
PciSI	GCTCTTC	1 cut(s)	1989
PctI	GAATGC	2 cut(s)	976, 1079
PdmI	GAANNNNTTC	1 cut(s)	1309
PflFI	GACNNNGTC	1 cut(s)	739
PleI	GAGTC	1 cut(s)	1075
PpsI	GAGTC	1 cut(s)	1075
Ppu21I	YACGTR	2 cut(s)	1501, 2156
PshBI	ATTAAT	1 cut(s)	384
Psp124BI	GAGCTC	2 cut(s)	1128, 1546
PspN4I	GGNNCC	1 cut(s)	724
PspPI	GGNCC	2 cut(s)	1086, 1680
PstI	CTGCAG	1 cut(s)	1137
PstNI	CAGNNNCTG	2 cut(s)	1655, 1822
PsuI	RGATCY	3 cut(s)	43, 1467, 2427
PsyI	GACNNNGTC	1 cut(s)	739
PvuII	CAGCTG	3 cut(s)	118, 1132, 1184
RsaI	GTAC	6 cut(s)	421, 921, 938, 1354, 1484, 2350
RsaNI	GTAC	6 cut(s)	420, 920, 937, 1353, 1483, 2349
RseI	CAYNNNNRTG	1 cut(s)	969
SacI	GAGCTC	2 cut(s)	1128, 1546
SapI	GCTCTTC	1 cut(s)	1989
Sau3AI	GATC	7 cut(s)	43, 589, 619, 952, 1410, 1467, 2427
Sau96I	GGNCC	2 cut(s)	1086, 1680
SchI	GAGTC	1 cut(s)	1076
SduI	GDGCHC	2 cut(s)	1128, 1546
SfaNI	GCATC	6 cut(s)	439, 693, 704, 1681, 1703, 1828
SfcI	CTRYAG	2 cut(s)	279, 1133
SinI	GGWCC	1 cut(s)	1086
SmiMI	CAYNNNNRTG	1 cut(s)	969
SmlI	CTYRAG	3 cut(s)	1421, 1986, 2209
SmoI	CTYRAG	3 cut(s)	1421, 1986, 2209
SnaBI	TACGTA	1 cut(s)	1501
SpeI	ACTAGT	1 cut(s)	664
SsiI	CCGC	6 cut(s)	107, 216, 239, 826, 1369, 1557
SspI	AATATT	2 cut(s)	2221, 2329
SstI	GAGCTC	2 cut(s)	1128, 1546
TaaI	ACNGT	1 cut(s)	936
TaiI	ACGT	3 cut(s)	511, 1503, 2158
TaqI	TCGA	2 cut(s)	624, 1247
TaqII	GACCGA	2 cut(s)	2374, 2456
TatI	WGTACW	3 cut(s)	919, 936, 2348
TauI	GCSGC	2 cut(s)	828, 1559
TscAI	CASTG	4 cut(s)	1289, 1660, 2117, 2342
TseFI	GTSAC	4 cut(s)	741, 1834, 2432, 2474
TseI	GCWGC	8 cut(s)	818, 1132, 1181, 1610, 1628, 1649, 1828, 2285
Tsp45I	GTSAC	4 cut(s)	741, 1834, 2432, 2474
TspGWI	ACGGA	5 cut(s)	216, 419, 1317, 2420, 2451
TspRI	CASTG	4 cut(s)	1289, 1660, 2117, 2342
Tth111I	GACNNNGTC	1 cut(s)	739
VpaK11BI	GGWCC	1 cut(s)	1086
VspI	ATTAAT	1 cut(s)	384
XapI	RAATTY	4 cut(s)	836, 1980, 2315, 2419
XbaI	TCTAGA	3 cut(s)	929, 1591, 2065
XceI	RCATGY	1 cut(s)	100
XmnI	GAANNNNTTC	1 cut(s)	1309
Zsp2I	ATGCAT	3 cut(s)	708, 1576, 1696

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.