Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0010574

Physical Location & Seq

Genomic Coordinates Reverse (-)

27483097 .. 27488574

Gene Structure 5478 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1211200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2118 bp

ATGTCGGCTCCATTGACGCCTTCGAAGAGACCACATGAGGAGAGCGTTACGGAGACTAATGGGAAAGGGAAGTTGCAGAAGTCTGAAAATTTAGATTCTAAGCTCCCCGCTGGCAATGTTGCTTTCCGGGTGCTGTGCCCTGCGTCCAAAGTTGATAATGTCACTGGTGAAGGTGGCTCTGTCATATCACAGATTTTTCAAGAAACTCTTGTAAAGGTCAGAGTCGAGGAACCTGTTCCGGGATGCGATGAAAGAGTTATTATCGTGGGTTCTGATAGAGAGAATGATGTTGGTAGCGAGCATAGAAAGGAGGACGGCATTGAAGATGCAAATGTGGATGAAAAGCAAGATGACGCTAAGGAGCCAAGAGAGAAGGATGAAACTCAGGAATCTGTTCCTATCGAAGATTCAAAATCTGATACTGAAGATTCAGTAACCCCATCTTTGCAGAAGGCTCTGCAACTTGTATTTGAGAGAATAGTTGAAGGGGAAACGGAAACCGATGGAGGGGATGAAGAAAGTAATAAGTCTTTGACATTTGTTTGTAGGTTACTAGTCCTATCATCTCAAGTGGGATGCCTCTTGGGAAAGGGTGGTAGTGTTATCAAACAAATGTCATCTGAAAGTGGGGCACAAATCCGGATCCTTCCCAGGGATAAACTTCCATTATGTGCTTCAGCTTCGGATGAACTAGTGCAGATTACGGGGAACATTGATGCAGCTAGGAAAGCCATTGAATCTGTTTCCCAGCAGCTTTTGGAGAATCTGCCTCGCGATAGTGATGATTCCCTTCCCACCACCGGGCCATCATCTAATTCATTCGGTCAACCGCTTCCAAGACCAGAAATATATCCACCACCTAACCATTCTCAAGGAGCTCCTGATATTGATCAGCCTGGAGATTTTGCTGATTCCCATTCTGCTGCTCATCGATTAAACCCTAAATTACATGGAAGTGGCATCCCTCGTAGGATGAAGCCTTCTCAGGAAGTCCTGACTTTCCGCTTATTGTGCCCTGAAGAGAGAGTAGGCGGTGTAATTGGAAAGGGAGGAACCATAATAAAGACACTCAAGCAAGAGACAGGCTGTGAAATCAAGGTTATGGAAGGCGTGCCTGACTCAGACGATCGTCTTATTGTTATATCTGGTCCTGCGCACCCAGATGATAGGATATCACCTGTCCAAGATGCCGTTATTCTTGTGCAAAGTAGGATATTCAGGGCTATACCTAATATCAAGGAACAATCCATGATGGCGAGGCTTCTTGTTTTCTCTAATCAAATTGGTTGTCTCCTTGGAAAGGGTGGTGCCATCATTGCAGAGATGAGAAAGTTATCTAGGGCACATATCCGCATAATGGGGAAGGATCAAATTCCAAAATGCGCTTCAGTTGATGAAGAAGTTGTTCAGATAAATGGAGAATTTGAAGCTGTTAAAGATGCTCTTCTGCAGATCACCACTAGGTTGCGGCTGCACTTTTTTGGTGATGCATTTCCTTCTATAAATTATCATCAAAATCCTGCGTTTTTGGATCAACCACCATTTCAATCATATCTGAGGAGGGACTTCTCACCTCCAAGAATGCATTCTAATTTAGGCCCATCGTTTCACAAGTTCGATGCTGTTGGTGGCCTTCCCCCTCATGGTAGTTTTCATCCTCATGATGATCGTGCTCCTTTTATGCACAATATTCATAGGTTAGGTGCCCCCCCACATTTATCCGAGAGAAGACCTTGGGGTCCGCAGGGACTCCATGAAGGTGGTGGTCCAGTAGGCTTGCCAAACTTTCCAGGCGCTCCTCAAAGAAGAATCCCAGGGTTTGGAGGAACCCAACCAGCTATAGTTACCAACACCACTGTCGAAGTTGTTGTGCCTAGTTCCCTTGTTCCTGTTATCAATGGAGAAGATGGTGAATGCTTGAAACAAATACGTCAGATTTCTGATGCAAAAGTTACCATTACTGAGCCCAAGCCTGGAGCAGTTGAAACCGTGATTATAATATCTGGAACACCTGAACAAACGCATGCTGCGCAGAGCCTTATTCAGGCATTTGTAATGATAGCTCGGCTGAAGATGAAGATTGAGATCAATGTTGCAGTTCCGAGAAGATTTATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KH_1

Protein Analysis

706

Amino Acids

76.67

Weight (kDa)

5.69

Isoelectric Point (pI)

54.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KH_1	PF00013	42 - 94	1.7e-07	KH domain
KH_1	PF00013	182 - 248	1.3e-13	KH domain
KH_1	PF00013	333 - 385	2.2e-14	KH domain
KH_1	PF00013	420 - 485	4.8e-12	KH domain
KH_1	PF00013	619 - 682	4.6e-11	KH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010574)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g33520 FvH4_5g33520
malus_domestica	MD08G1211200.v1.1
prunus_persica	Prupe.1G543200_v2.0.a1 Prupe.1G543200_v2.0.a1 Prupe.1G543200_v2.0.a1
pyrus_communis	pycom08g18200 pycom15g35520
rosa_chinensis	RchiOBHm_Chr7g0232321
rosa_laevigata	RLG00000001357
rosa_multiflora	Rmu_co8258439.1_g000001 Rmu_sc0004136.1_g000004 Rmu_sc0034864.1_g000001
rosa_roxburghii	Rroxscaffold_3G00228470
rosa_rugosa	Rorug07G0272500 Rorug07G0272600
rosa_samantha	Rh7AG426500 Rh7BG400200 Rh7CG446200 Rh7DG417700
rosa_wichuraiana	Rw7G035360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1997
AasI	GACNNNNNNGTC	1 cut(s)	1737
Acc16I	TGCGCA	2 cut(s)	1155, 2031
AccB1I	GGYRCC	2 cut(s)	1307, 1703
AccB7I	CCANNNNNTGG	1 cut(s)	1820
AccII	CGCG	1 cut(s)	774
AccIII	TCCGGA	1 cut(s)	639
AciI	CCGC	7 cut(s)	108, 830, 1003, 1032, 1351, 1468, 1742
AclWI	GGATC	4 cut(s)	637, 650, 1374, 1539
AcsI	RAATTY	3 cut(s)	88, 1371, 1421
AcuI	CTGAAG	5 cut(s)	444, 660, 1038, 1371, 2090
AcyI	GRCGYC	1 cut(s)	17
AgsI	TTSAA	9 cut(s)	200, 323, 411, 485, 737, 1427, 1547, 1921, 1985
AhlI	ACTAGT	2 cut(s)	553, 691
AjnI	CCWGG	5 cut(s)	650, 895, 1789, 1813, 1972
AjuI	GAANNNNNNNTTGG	4 cut(s)	1369, 1401, 1570, 1602
AluBI	AGCT	8 cut(s)	103, 680, 722, 754, 878, 1430, 1838, 2063
AluI	AGCT	8 cut(s)	103, 680, 722, 754, 878, 1430, 1838, 2063
Alw21I	GWGCWC	2 cut(s)	880, 1675
Alw26I	GTCTC	4 cut(s)	22, 47, 1073, 1295
AlwI	GGATC	4 cut(s)	637, 650, 1374, 1539
Aor13HI	TCCGGA	1 cut(s)	639
AoxI	GGCC	3 cut(s)	803, 1597, 1630
ApeKI	GCWGC	5 cut(s)	719, 751, 923, 1471, 2027
ApoI	RAATTY	3 cut(s)	88, 1371, 1421
ArsI	GACNNNNNNTTYG	2 cut(s)	1273, 1305
Asp700I	GAANNNNTTC	4 cut(s)	234, 393, 1404, 1585
AspLEI	GCGC	4 cut(s)	1156, 1385, 1796, 2032
AspS9I	GGNCC	5 cut(s)	803, 1148, 1598, 1739, 1766
AsuC2I	CCSGG	3 cut(s)	128, 240, 802
AsuHPI	GGTGA	6 cut(s)	179, 1167, 1447, 1496, 1563, 1922
AsuII	TTCGAA	1 cut(s)	23
AvaII	GGWCC	3 cut(s)	1148, 1739, 1766
BaeGI	GKGCMC	5 cut(s)	140, 634, 1016, 1345, 1708
BamHI	GGATCC	1 cut(s)	642
BanI	GGYRCC	2 cut(s)	1307, 1703
BanII	GRGCYC	2 cut(s)	880, 1968
BbsI	GAAGAC	1 cut(s)	1735
Bbv12I	GWGCWC	2 cut(s)	880, 1675
BbvI	GCAGC	5 cut(s)	731, 763, 910, 1458, 2014
BccI	CCATC	7 cut(s)	448, 497, 814, 1246, 1319, 1609, 1901
BceAI	ACGGC	2 cut(s)	331, 1175
BciT130I	CCWGG	5 cut(s)	652, 897, 1791, 1815, 1974
BclI	TGATCA	1 cut(s)	889
BcnI	CCSGG	3 cut(s)	128, 240, 802
BcoDI	GTCTC	4 cut(s)	22, 47, 1073, 1295
BcuI	ACTAGT	2 cut(s)	553, 691
BfaI	CTAG	6 cut(s)	554, 692, 723, 1338, 1461, 1875
BfmI	CTRYAG	2 cut(s)	1448, 1839
BfoI	RGCGCY	1 cut(s)	1797
BisI	GCNGC	6 cut(s)	720, 752, 924, 1469, 1472, 2028
BlsI	GCNGC	6 cut(s)	721, 753, 925, 1470, 1473, 2029
Bme1390I	CCNGG	8 cut(s)	128, 240, 652, 802, 897, 1791, 1815, 1974
Bme18I	GGWCC	3 cut(s)	1148, 1739, 1766
BmgT120I	GGNCC	5 cut(s)	803, 1148, 1598, 1739, 1766
BmiI	GGNNCC	9 cut(s)	9, 231, 363, 644, 1054, 1309, 1705, 1740, 1828
BmrFI	CCNGG	8 cut(s)	128, 240, 652, 802, 897, 1791, 1815, 1974
BoxI	GACNNNNGTC	1 cut(s)	1128
BpiI	GAAGAC	1 cut(s)	1735
BpmI	CTGGAG	2 cut(s)	918, 1995
Bpu10I	CCTNAGC	1 cut(s)	357
Bpu14I	TTCGAA	1 cut(s)	23
BpuEI	CTTGAG	3 cut(s)	552, 855, 1055
BpuMI	CCSGG	3 cut(s)	128, 240, 802
Bsa29I	ATCGAT	1 cut(s)	931
BsaBI	GATNNNNATC	2 cut(s)	888, 2084
BsaHI	GRCGYC	1 cut(s)	17
BsaI	GGTCTC	1 cut(s)	22
BsaJI	CCNNGG	6 cut(s)	650, 651, 1294, 1733, 1813, 1814
BsaWI	WCCGGW	1 cut(s)	639
Bse1I	ACTGG	2 cut(s)	169, 1769
Bse3DI	GCAATG	2 cut(s)	121, 1314
Bse8I	GATNNNNATC	2 cut(s)	888, 2084
BseAI	TCCGGA	1 cut(s)	639
BseBI	CCWGG	5 cut(s)	652, 897, 1791, 1815, 1974
BseCI	ATCGAT	1 cut(s)	931
BseDI	CCNNGG	6 cut(s)	650, 651, 1294, 1733, 1813, 1814
BseGI	GGATG	9 cut(s)	248, 343, 382, 517, 581, 691, 960, 978, 1654
BseJI	GATNNNNATC	2 cut(s)	888, 2084
BseMI	GCAATG	2 cut(s)	121, 1314
BseMII	CTCAG	5 cut(s)	398, 998, 1134, 1547, 1953
BseNI	ACTGG	2 cut(s)	169, 1769
BseRI	GAGGAG	3 cut(s)	53, 1573, 1788
BseSI	GKGCMC	5 cut(s)	140, 634, 1016, 1345, 1708
BseXI	GCAGC	5 cut(s)	731, 763, 910, 1458, 2014
BseYI	CCCAGC	1 cut(s)	747
BsgI	GTGCAG	2 cut(s)	716, 1457
Bsh1236I	CGCG	1 cut(s)	774
Bsh1285I	CGRYCG	1 cut(s)	1129
BshFI	GGCC	3 cut(s)	805, 1599, 1632
BshNI	GGYRCC	2 cut(s)	1307, 1703
BshVI	ATCGAT	1 cut(s)	931
BsiEI	CGRYCG	1 cut(s)	1129
BsiHKAI	GWGCWC	2 cut(s)	880, 1675
BsiSI	CCGG	4 cut(s)	127, 239, 640, 801
BslFI	GGGAC	2 cut(s)	1577, 1761
BsmAI	GTCTC	4 cut(s)	22, 47, 1073, 1295
BsmFI	GGGAC	2 cut(s)	1577, 1761
BsmI	GAATGC	3 cut(s)	1585, 1587, 1919
BsnI	GGCC	3 cut(s)	805, 1599, 1632
Bso31I	GGTCTC	1 cut(s)	22
Bsp119I	TTCGAA	1 cut(s)	23
Bsp1286I	GDGCHC	8 cut(s)	140, 634, 880, 1016, 1345, 1675, 1708, 1968
Bsp13I	TCCGGA	1 cut(s)	639
Bsp143I	GATC	8 cut(s)	642, 889, 1126, 1366, 1452, 1531, 1666, 2085
Bsp68I	TCGCGA	1 cut(s)	774
BspACI	CCGC	7 cut(s)	108, 830, 1003, 1032, 1351, 1468, 1742
BspANI	GGCC	3 cut(s)	805, 1599, 1632
BspCNI	CTCAG	5 cut(s)	397, 997, 1133, 1548, 1954
BspDI	ATCGAT	1 cut(s)	931
BspEI	TCCGGA	1 cut(s)	639
BspFNI	CGCG	1 cut(s)	774
BspHI	TCATGA	1 cut(s)	1660
BspLI	GGNNCC	9 cut(s)	9, 231, 363, 644, 1054, 1309, 1705, 1740, 1828
BspMAI	CTGCAG	1 cut(s)	1452
BspPI	GGATC	4 cut(s)	637, 650, 1374, 1539
BspQI	GCTCTTC	1 cut(s)	1449
BspT104I	TTCGAA	1 cut(s)	23
BspT107I	GGYRCC	2 cut(s)	1307, 1703
BspTNI	GGTCTC	1 cut(s)	22
BsrDI	GCAATG	2 cut(s)	121, 1314
BsrI	ACTGG	2 cut(s)	169, 1769
BssECI	CCNNGG	6 cut(s)	650, 651, 1294, 1733, 1813, 1814
BssMI	GATC	8 cut(s)	642, 889, 1126, 1366, 1452, 1531, 1666, 2085
BssNI	GRCGYC	1 cut(s)	17
BssT1I	CCWWGG	2 cut(s)	1294, 1733
Bst2UI	CCWGG	5 cut(s)	652, 897, 1791, 1815, 1974
Bst4CI	ACNGT	2 cut(s)	1858, 1990
Bst6I	CTCTTC	3 cut(s)	20, 1014, 1449
BstACI	GRCGYC	1 cut(s)	17
BstBI	TTCGAA	1 cut(s)	23
BstC8I	GCNNGC	5 cut(s)	112, 299, 1112, 1778, 2025
BstDEI	CTNAG	7 cut(s)	99, 357, 384, 984, 1120, 1556, 1962
BstENI	CCTNNNNNAGG	2 cut(s)	1298, 2042
BstF5I	GGATG	9 cut(s)	248, 343, 382, 517, 581, 691, 960, 978, 1654
BstFNI	CGCG	1 cut(s)	774
BstH2I	RGCGCY	1 cut(s)	1797
BstHHI	GCGC	4 cut(s)	1156, 1385, 1796, 2032
BstKTI	GATC	8 cut(s)	645, 892, 1129, 1369, 1455, 1534, 1669, 2088
BstMAI	GTCTC	4 cut(s)	22, 47, 1073, 1295
BstMBI	GATC	8 cut(s)	642, 889, 1126, 1366, 1452, 1531, 1666, 2085
BstMCI	CGRYCG	1 cut(s)	1129
BstMWI	GCNNNNNNNGC	4 cut(s)	728, 1011, 1316, 2029
BstNI	CCWGG	5 cut(s)	652, 897, 1791, 1815, 1974
BstNSI	RCATGY	1 cut(s)	2027
BstPAI	GACNNNNGTC	1 cut(s)	1128
BstSCI	CCNGG	8 cut(s)	126, 238, 650, 800, 895, 1789, 1813, 1972
BstSFI	CTRYAG	2 cut(s)	1448, 1839
BstSLI	GKGCMC	5 cut(s)	140, 634, 1016, 1345, 1708
BstUI	CGCG	1 cut(s)	774
BstV1I	GCAGC	5 cut(s)	731, 763, 910, 1458, 2014
BstV2I	GAAGAC	1 cut(s)	1735
BstX2I	RGATCY	1 cut(s)	642
BstXI	CCANNNNNNTGG	1 cut(s)	1760
BstYI	RGATCY	1 cut(s)	642
Bsu15I	ATCGAT	1 cut(s)	931
BsuRI	GGCC	3 cut(s)	805, 1599, 1632
BsuTUI	ATCGAT	1 cut(s)	931
BtgZI	GCGATG	1 cut(s)	261
BtsCI	GGATG	9 cut(s)	248, 343, 382, 517, 581, 691, 960, 978, 1654
BtsIMutI	CAGTG	2 cut(s)	162, 1854
BtuMI	TCGCGA	1 cut(s)	774
Cac8I	GCNNGC	5 cut(s)	112, 299, 1112, 1778, 2025
CciI	TCATGA	1 cut(s)	1660
CfoI	GCGC	4 cut(s)	1156, 1385, 1796, 2032
Cfr13I	GGNCC	5 cut(s)	803, 1148, 1598, 1739, 1766
ClaI	ATCGAT	1 cut(s)	931
CseI	GACGC	3 cut(s)	25, 132, 362
CviAII	CATG	7 cut(s)	35, 950, 1249, 1643, 1661, 1754, 2024
DdeI	CTNAG	7 cut(s)	99, 357, 384, 984, 1120, 1556, 1962
DpnI	GATC	8 cut(s)	644, 891, 1128, 1368, 1454, 1533, 1668, 2087
DpnII	GATC	8 cut(s)	642, 889, 1126, 1366, 1452, 1531, 1666, 2085
DrdI	GACNNNNNNGTC	1 cut(s)	1737
DseDI	GACNNNNNNGTC	1 cut(s)	1737
Eam1104I	CTCTTC	3 cut(s)	20, 1014, 1449
EarI	CTCTTC	3 cut(s)	20, 1014, 1449
Ecl136II	GAGCTC	1 cut(s)	878
Eco130I	CCWWGG	2 cut(s)	1294, 1733
Eco24I	GRGCYC	2 cut(s)	880, 1968
Eco31I	GGTCTC	1 cut(s)	22
Eco32I	GATATC	1 cut(s)	1173
Eco47I	GGWCC	3 cut(s)	1148, 1739, 1766
Eco53kI	GAGCTC	1 cut(s)	878
Eco57I	CTGAAG	5 cut(s)	444, 660, 1038, 1371, 2090
EcoICRI	GAGCTC	1 cut(s)	878
EcoNI	CCTNNNNNAGG	2 cut(s)	1298, 2042
EcoRII	CCWGG	5 cut(s)	650, 895, 1789, 1813, 1972
EcoRV	GATATC	1 cut(s)	1173
EcoT14I	CCWWGG	2 cut(s)	1294, 1733
EcoT22I	ATGCAT	2 cut(s)	1492, 1587
EcoT38I	GRGCYC	2 cut(s)	880, 1968
ErhI	CCWWGG	2 cut(s)	1294, 1733
FaeI	CATG	7 cut(s)	38, 953, 1252, 1646, 1664, 1757, 2027
FalI	AAGNNNNNCTT	4 cut(s)	192, 224, 1428, 1460
FaqI	GGGAC	2 cut(s)	1577, 1761
FatI	CATG	7 cut(s)	34, 949, 1248, 1642, 1660, 1753, 2023
FauI	CCCGC	1 cut(s)	115
FbaI	TGATCA	1 cut(s)	889
Fnu4HI	GCNGC	6 cut(s)	720, 752, 924, 1469, 1472, 2028
FokI	GGATG	9 cut(s)	255, 350, 389, 524, 588, 698, 947, 985, 1641
FriOI	GRGCYC	2 cut(s)	880, 1968
Fsp4HI	GCNGC	6 cut(s)	720, 752, 924, 1469, 1472, 2028
FspBI	CTAG	6 cut(s)	554, 692, 723, 1338, 1461, 1875
FspI	TGCGCA	2 cut(s)	1155, 2031
GlaI	GCGC	4 cut(s)	1155, 1384, 1795, 2031
GluI	GCNGC	6 cut(s)	720, 752, 924, 1469, 1472, 2028
GsaI	CCCAGC	1 cut(s)	751
GsuI	CTGGAG	2 cut(s)	918, 1995
HaeII	RGCGCY	1 cut(s)	1797
HaeIII	GGCC	3 cut(s)	805, 1599, 1632
HapII	CCGG	4 cut(s)	127, 239, 640, 801
HgaI	GACGC	3 cut(s)	25, 132, 362
HhaI	GCGC	4 cut(s)	1156, 1385, 1796, 2032
Hin1I	GRCGYC	1 cut(s)	17
Hin1II	CATG	7 cut(s)	38, 953, 1252, 1646, 1664, 1757, 2027
Hin6I	GCGC	4 cut(s)	1154, 1383, 1794, 2030
HinP1I	GCGC	4 cut(s)	1154, 1383, 1794, 2030
HincII	GTYRAC	1 cut(s)	827
HindII	GTYRAC	1 cut(s)	827
HpaII	CCGG	4 cut(s)	127, 239, 640, 801
HphI	GGTGA	6 cut(s)	179, 1167, 1447, 1496, 1563, 1922
Hpy166II	GTNNAC	1 cut(s)	827
Hpy188III	TCNNGA	9 cut(s)	200, 386, 640, 773, 881, 986, 994, 1661, 2004
Hpy8I	GTNNAC	1 cut(s)	827
HpyCH4III	ACNGT	2 cut(s)	1858, 1990
HpyCH4IV	ACGT	1 cut(s)	1930
HpyF10VI	GCNNNNNNNGC	4 cut(s)	728, 1011, 1316, 2029
HpyF3I	CTNAG	7 cut(s)	99, 357, 384, 984, 1120, 1556, 1962
HpySE526I	ACGT	1 cut(s)	1930
Hsp92I	GRCGYC	1 cut(s)	17
Hsp92II	CATG	7 cut(s)	38, 953, 1252, 1646, 1664, 1757, 2027
HspAI	GCGC	4 cut(s)	1154, 1383, 1794, 2030
Kpn2I	TCCGGA	1 cut(s)	639
Ksp22I	TGATCA	1 cut(s)	889
Kzo9I	GATC	8 cut(s)	642, 889, 1126, 1366, 1452, 1531, 1666, 2085
LguI	GCTCTTC	1 cut(s)	1449
LmnI	GCTCC	8 cut(s)	13, 108, 361, 875, 883, 1678, 1801, 1976
Lsp1109I	GCAGC	5 cut(s)	731, 763, 910, 1458, 2014
MaeI	CTAG	6 cut(s)	554, 692, 723, 1338, 1461, 1875
MaeII	ACGT	1 cut(s)	1930
MaeIII	GTNAC	6 cut(s)	46, 160, 433, 549, 1843, 1951
MalI	GATC	8 cut(s)	644, 891, 1128, 1368, 1454, 1533, 1668, 2087
MboI	GATC	8 cut(s)	642, 889, 1126, 1366, 1452, 1531, 1666, 2085
MflI	RGATCY	1 cut(s)	642
MhlI	GDGCHC	8 cut(s)	140, 634, 880, 1016, 1345, 1675, 1708, 1968
MluCI	AATT	9 cut(s)	88, 814, 944, 1038, 1281, 1371, 1421, 1504, 1591
MlyI	GAGTC	3 cut(s)	231, 1112, 1743
Mph1103I	ATGCAT	2 cut(s)	1492, 1587
MroI	TCCGGA	1 cut(s)	639
MroXI	GAANNNNTTC	4 cut(s)	234, 393, 1404, 1585
MseI	TTAA	2 cut(s)	935, 1434
MslI	CAYNNNNRTG	4 cut(s)	954, 1161, 1659, 1758
MspA1I	CMGCKG	1 cut(s)	110
MspI	CCGG	4 cut(s)	127, 239, 640, 801
MspR9I	CCNGG	8 cut(s)	128, 240, 652, 802, 897, 1791, 1815, 1974
Mva1269I	GAATGC	3 cut(s)	1585, 1587, 1919
MvaI	CCWGG	5 cut(s)	652, 897, 1791, 1815, 1974
MvnI	CGCG	1 cut(s)	774
MwoI	GCNNNNNNNGC	4 cut(s)	728, 1011, 1316, 2029
NciI	CCSGG	3 cut(s)	128, 240, 802
NdeII	GATC	8 cut(s)	642, 889, 1126, 1366, 1452, 1531, 1666, 2085
NlaIII	CATG	7 cut(s)	38, 953, 1252, 1646, 1664, 1757, 2027
NlaIV	GGNNCC	9 cut(s)	9, 231, 363, 644, 1054, 1309, 1705, 1740, 1828
NmeAIII	GCCGAG	1 cut(s)	2044
NmuCI	GTSAC	1 cut(s)	160
NruI	TCGCGA	1 cut(s)	774
NsbI	TGCGCA	2 cut(s)	1155, 2031
NsiI	ATGCAT	2 cut(s)	1492, 1587
NspI	RCATGY	1 cut(s)	2027
NspV	TTCGAA	1 cut(s)	23
PaeI	GCATGC	1 cut(s)	2027
PagI	TCATGA	1 cut(s)	1660
PasI	CCCWGGG	2 cut(s)	651, 1814
PciSI	GCTCTTC	1 cut(s)	1449
PctI	GAATGC	3 cut(s)	1585, 1587, 1919
PdmI	GAANNNNTTC	4 cut(s)	234, 393, 1404, 1585
PfeI	GAWTC	9 cut(s)	95, 389, 407, 428, 737, 763, 785, 911, 1809
PflMI	CCANNNNNTGG	1 cut(s)	1820
PfoI	TCCNGGA	1 cut(s)	238
PkrI	GCNGC	6 cut(s)	721, 753, 925, 1470, 1473, 2029
Ple19I	CGATCG	1 cut(s)	1129
PleI	GAGTC	3 cut(s)	230, 1112, 1743
PpsI	GAGTC	3 cut(s)	230, 1112, 1743
PshAI	GACNNNNGTC	1 cut(s)	1128
PsiI	TTATAA	1 cut(s)	1997
Psp124BI	GAGCTC	1 cut(s)	880
Psp6I	CCWGG	5 cut(s)	650, 895, 1789, 1813, 1972
PspFI	CCCAGC	1 cut(s)	747
PspGI	CCWGG	5 cut(s)	650, 895, 1789, 1813, 1972
PspN4I	GGNNCC	9 cut(s)	9, 231, 363, 644, 1054, 1309, 1705, 1740, 1828
PspPI	GGNCC	5 cut(s)	803, 1148, 1598, 1739, 1766
PstI	CTGCAG	1 cut(s)	1452
PsuI	RGATCY	1 cut(s)	642
PvuI	CGATCG	1 cut(s)	1129
RruI	TCGCGA	1 cut(s)	774
RseI	CAYNNNNRTG	4 cut(s)	954, 1161, 1659, 1758
SacI	GAGCTC	1 cut(s)	880
SapI	GCTCTTC	1 cut(s)	1449
SaqAI	TTAA	2 cut(s)	935, 1434
SatI	GCNGC	6 cut(s)	720, 752, 924, 1469, 1472, 2028
Sau3AI	GATC	8 cut(s)	642, 889, 1126, 1366, 1452, 1531, 1666, 2085
Sau96I	GGNCC	5 cut(s)	803, 1148, 1598, 1739, 1766
SchI	GAGTC	3 cut(s)	231, 1112, 1743
ScrFI	CCNGG	8 cut(s)	128, 240, 652, 802, 897, 1791, 1815, 1974
SduI	GDGCHC	8 cut(s)	140, 634, 880, 1016, 1345, 1675, 1708, 1968
SfcI	CTRYAG	2 cut(s)	1448, 1839
SfuI	TTCGAA	1 cut(s)	23
SinI	GGWCC	3 cut(s)	1148, 1739, 1766
SmiMI	CAYNNNNRTG	4 cut(s)	954, 1161, 1659, 1758
SmlI	CTYRAG	3 cut(s)	567, 870, 1070
SmoI	CTYRAG	3 cut(s)	567, 870, 1070
SpeI	ACTAGT	2 cut(s)	553, 691
SphI	GCATGC	1 cut(s)	2027
Sse9I	AATT	9 cut(s)	88, 814, 944, 1038, 1281, 1371, 1421, 1504, 1591
SsiI	CCGC	7 cut(s)	108, 830, 1003, 1032, 1351, 1468, 1742
SspI	AATATT	1 cut(s)	1690
SspMI	CTAG	6 cut(s)	554, 692, 723, 1338, 1461, 1875
SstI	GAGCTC	1 cut(s)	880
StyD4I	CCNGG	8 cut(s)	126, 238, 650, 800, 895, 1789, 1813, 1972
StyI	CCWWGG	2 cut(s)	1294, 1733
TaaI	ACNGT	2 cut(s)	1858, 1990
TaiI	ACGT	1 cut(s)	1933
TaqI	TCGA	6 cut(s)	23, 225, 402, 931, 1617, 1860
TaqII	GACCGA	1 cut(s)	812
TasI	AATT	9 cut(s)	88, 814, 944, 1038, 1281, 1371, 1421, 1504, 1591
TauI	GCSGC	1 cut(s)	1471
TfiI	GAWTC	9 cut(s)	95, 389, 407, 428, 737, 763, 785, 911, 1809
Tru1I	TTAA	2 cut(s)	935, 1434
Tru9I	TTAA	2 cut(s)	935, 1434
TscAI	CASTG	2 cut(s)	169, 1861
TseFI	GTSAC	1 cut(s)	160
TseI	GCWGC	5 cut(s)	719, 751, 923, 1471, 2027
Tsp45I	GTSAC	1 cut(s)	160
TspGWI	ACGGA	2 cut(s)	65, 509
TspRI	CASTG	2 cut(s)	169, 1861
Van91I	CCANNNNNTGG	1 cut(s)	1820
VpaK11BI	GGWCC	3 cut(s)	1148, 1739, 1766
XagI	CCTNNNNNAGG	2 cut(s)	1298, 2042
XapI	RAATTY	3 cut(s)	88, 1371, 1421
XceI	RCATGY	1 cut(s)	2027
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1760
XmnI	GAANNNNTTC	4 cut(s)	234, 393, 1404, 1585
XspI	CTAG	6 cut(s)	554, 692, 723, 1338, 1461, 1875
Zsp2I	ATGCAT	2 cut(s)	1492, 1587

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.