Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0010574

Physical Location & Seq

Genomic Coordinates Reverse (-)

35573196 .. 35575567

Gene Structure 2372 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom15g35520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1635 bp

ATGTCGGCTCCATTGACGCCGTCGAAGAGACCACATGAGGAGAGCCTTGCAGAGACTAATGGGAAAGGGAAGTTGCAGAAGTCTGAAAATCAAGCTTCTGAACTCTCCCCTGGCAATGTTGCTTTCAGGGTACTGTGCCCTGCGTCCAAAGTTGATAATGTCACTGGGGAAGGTGGCTCTGTCATATCACAGATTTTTCAAGAAACTTTTGTAAGGGTCAGAATTGAGGAAGCTGTCCCGGGATGTGATGAAAGGGTTATTGTTGTGGGTTCTGATGGAGAGAATGATGTTGGTAGCGAGCATAGAAAGGAGGATGGCGTTGAAGAGGCAAATGTGGATGAAAAGCATGATGGTACTAGGGAACCAAGAGAAAAGCATGATGTATCTCCATCTTTGCAGAGGGCTCTGAAACTTGTTTTTGAGAGAATAGTTGAAGGGGAAACAGAGAGCGATGGAGCAGATGAAGAAAGTAATAAGTCTTTGACATTTGTTTGCAGATTACTAGTCCTTTCATCTCAAGTGGGATGCCTCTTGGGAAAGGGTGGTAGTGTTATCAAACAAATGTCATCTGAAAGTGGGGCACAAATCCGGATCCTTCCCACAGATAAACTTCCGTCATGTGCGGCAGTTTGCGATGAACTAGTTCAGATTTCGGGGAACATTGATGCAGTTGGGAAAGCTATTGAATATGTTTCCCGGCAGCTTTTGGATAATCTGCCTCATGATAGTGACGCTTCCCTTGCCAAGACCACTGGGACATCATCTAATTCATTCGGTCAACCTCTTCTAAGACCCGAAATACATCCATCACCTAACCACTCCGAAGGGGCTCCTGATATTTATCAGCCTGGAGATTTTGCTGCTCATCCAGTAAACCCTAAATTACATGGAAGTGGCATCCCTGGTAGGATGAAGCCTTCTCAGGAAGTCCTAACTTTCCGCTTATTGTGCCCTGAAGAGAGAGTAGGTGGTGTAATTGGCAAGGGAGGAACCATAATAAGGACACTTAAGCAAGAGACAGGCTGCGAAATCAGGGTTATGGAAGGTGTGCCTGACTCAGACGACCGTCTTATTGTTATATCTGGTCCTGTGCATCCAGATGATAGGATATCACCAGTCCAAGATGCCGTTATTCGTGTGCAAAGTAGGATATTCAGGGCTGTACCTAATAGCAAGGAACAATCCATGATGGCGAGGCTGCTTGTTTCCTCTAATCAAGTAGGTTGTCTCCTTGGAAAGGGTGGTACCATTATTGCGGAGATGAGAAAGTTATCTAGGGCCCATATCCGTATAATGGGGAAGGATCAAATTCTGAGATGGGCTTCAGTTGATGAAGAAGTTGTTCAGATAAATGGAGAATTTGAGGCCGTTAAAGATGCTCTTCTGCAGATTACCACTAGGTTGCAGCATAACCTTTTTCGTGACGCATTTCCTTCTATACATTATCATCAAAATCCTGCATTTTTGGATCAACCTCCATTTCAATCATATCTGAGGAGGGACTTCTCACCTCCAGGAATGCATTCTAATTTAGGCCCATCGTTGCACAAGTTTGATGCTGTTGGTGGCCTGCCCCCTCATGGTAGTTTTCATCCTCACGATCGATCGTGCTCCCTTTGTGCACAATATTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KH_1

Protein Analysis

545

Amino Acids

59.19

Weight (kDa)

5.78

Isoelectric Point (pI)

52.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KH_1	PF00013	42 - 90	2.4e-07	KH domain
KH_1	PF00013	165 - 231	2.6e-13	KH domain
KH_1	PF00013	312 - 363	3.5e-14	KH domain
KH_1	PF00013	399 - 464	2.3e-12	KH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010574)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g33520 FvH4_5g33520
malus_domestica	MD08G1211200.v1.1
prunus_persica	Prupe.1G543200_v2.0.a1 Prupe.1G543200_v2.0.a1 Prupe.1G543200_v2.0.a1
pyrus_communis	pycom08g18200 pycom15g35520
rosa_chinensis	RchiOBHm_Chr7g0232321
rosa_laevigata	RLG00000001357
rosa_multiflora	Rmu_co8258439.1_g000001 Rmu_sc0004136.1_g000004 Rmu_sc0034864.1_g000001
rosa_roxburghii	Rroxscaffold_3G00228470
rosa_rugosa	Rorug07G0272500 Rorug07G0272600
rosa_samantha	Rh7AG426500 Rh7BG400200 Rh7CG446200 Rh7DG417700
rosa_wichuraiana	Rw7G035360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1244
AccB1I	GGYRCC	1 cut(s)	1244
AccIII	TCCGGA	1 cut(s)	588
AciI	CCGC	3 cut(s)	623, 940, 1256
AclWI	GGATC	4 cut(s)	586, 599, 1311, 1476
AcsI	RAATTY	2 cut(s)	1308, 1358
AcuI	CTGAAG	2 cut(s)	975, 1308
AcyI	GRCGYC	1 cut(s)	17
AfaI	GTAC	4 cut(s)	132, 355, 1164, 1246
AfiI	CCNNNNNNNGG	4 cut(s)	999, 1237, 1294, 1580
AflII	CTTAAG	1 cut(s)	1007
AgsI	TTSAA	5 cut(s)	200, 323, 434, 686, 1484
AhlI	ACTAGT	2 cut(s)	502, 640
AjnI	CCWGG	4 cut(s)	109, 847, 901, 1513
AluBI	AGCT	4 cut(s)	95, 233, 680, 703
AluI	AGCT	4 cut(s)	95, 233, 680, 703
Alw21I	GWGCWC	2 cut(s)	1613, 1624
Alw26I	GTCTC	4 cut(s)	22, 47, 1010, 1232
Alw44I	GTGCAC	1 cut(s)	1620
AlwI	GGATC	4 cut(s)	586, 599, 1311, 1476
Ama87I	CYCGRG	1 cut(s)	238
Aor13HI	TCCGGA	1 cut(s)	588
AoxI	GGCC	4 cut(s)	1278, 1365, 1534, 1567
ApaI	GGGCCC	1 cut(s)	1282
ApaLI	GTGCAC	1 cut(s)	1620
ApeKI	GCWGC	5 cut(s)	700, 860, 1023, 1198, 1405
ApoI	RAATTY	2 cut(s)	1308, 1358
Asp700I	GAANNNNTTC	3 cut(s)	642, 1341, 1522
Asp718I	GGTACC	1 cut(s)	1244
AspS9I	GGNCC	4 cut(s)	1085, 1278, 1279, 1535
AsuC2I	CCSGG	3 cut(s)	239, 240, 697
AsuHPI	GGTGA	3 cut(s)	801, 1104, 1500
AvaI	CYCGRG	1 cut(s)	238
AvaII	GGWCC	1 cut(s)	1085
BaeGI	GKGCMC	5 cut(s)	140, 583, 953, 1282, 1624
BamHI	GGATCC	1 cut(s)	591
BanI	GGYRCC	1 cut(s)	1244
BanII	GRGCYC	3 cut(s)	406, 832, 1282
Bbv12I	GWGCWC	2 cut(s)	1613, 1624
BbvI	GCAGC	5 cut(s)	712, 847, 1010, 1185, 1417
BccI	CCATC	9 cut(s)	269, 308, 344, 397, 446, 814, 1183, 1311, 1546
BceAI	ACGGC	3 cut(s)	4, 1112, 1352
BciT130I	CCWGG	4 cut(s)	111, 849, 903, 1515
BcnI	CCSGG	3 cut(s)	239, 240, 697
BcoDI	GTCTC	4 cut(s)	22, 47, 1010, 1232
BcuI	ACTAGT	2 cut(s)	502, 640
BfaI	CTAG	5 cut(s)	357, 503, 641, 1275, 1398
BfmI	CTRYAG	1 cut(s)	1385
BfrI	CTTAAG	1 cut(s)	1007
BisI	GCNGC	6 cut(s)	624, 701, 861, 1024, 1199, 1406
BlsI	GCNGC	6 cut(s)	625, 702, 862, 1025, 1200, 1407
Bme1390I	CCNGG	7 cut(s)	111, 239, 240, 697, 849, 903, 1515
Bme18I	GGWCC	1 cut(s)	1085
BmeT110I	CYCGRG	1 cut(s)	238
BmgT120I	GGNCC	4 cut(s)	1085, 1278, 1279, 1535
BmiI	GGNNCC	7 cut(s)	9, 363, 593, 831, 991, 1246, 1280
BmrFI	CCNGG	7 cut(s)	111, 239, 240, 697, 849, 903, 1515
BmrI	ACTGGG	2 cut(s)	174, 762
BmsI	GCATC	7 cut(s)	515, 655, 906, 1102, 1114, 1366, 1546
BmuI	ACTGGG	2 cut(s)	174, 762
BoxI	GACNNNNGTC	1 cut(s)	1065
BpmI	CTGGAG	2 cut(s)	870, 1497
BpuEI	CTTGAG	1 cut(s)	501
BpuMI	CCSGG	3 cut(s)	239, 240, 697
Bsa29I	ATCGAT	1 cut(s)	1603
BsaBI	GATNNNNATC	1 cut(s)	840
BsaHI	GRCGYC	1 cut(s)	17
BsaI	GGTCTC	1 cut(s)	22
BsaJI	CCNNGG	4 cut(s)	109, 238, 901, 1231
BsaWI	WCCGGW	1 cut(s)	588
Bsc4I	CCNNNNNNNGG	4 cut(s)	999, 1237, 1294, 1580
Bse1I	ACTGG	4 cut(s)	169, 757, 869, 1115
Bse3DI	GCAATG	1 cut(s)	121
Bse8I	GATNNNNATC	1 cut(s)	840
BseAI	TCCGGA	1 cut(s)	588
BseBI	CCWGG	4 cut(s)	111, 849, 903, 1515
BseCI	ATCGAT	1 cut(s)	1603
BseDI	CCNNGG	4 cut(s)	109, 238, 901, 1231
BseJI	GATNNNNATC	1 cut(s)	840
BseLI	CCNNNNNNNGG	4 cut(s)	999, 1237, 1294, 1580
BseMI	GCAATG	1 cut(s)	121
BseMII	CTCAG	4 cut(s)	935, 1071, 1304, 1484
BseNI	ACTGG	4 cut(s)	169, 757, 869, 1115
BseRI	GAGGAG	2 cut(s)	53, 1510
BseSI	GKGCMC	5 cut(s)	140, 583, 953, 1282, 1624
BseXI	GCAGC	5 cut(s)	712, 847, 1010, 1185, 1417
Bsh1285I	CGRYCG	3 cut(s)	1066, 1603, 1607
BshFI	GGCC	4 cut(s)	1280, 1367, 1536, 1569
BshNI	GGYRCC	1 cut(s)	1244
BshVI	ATCGAT	1 cut(s)	1603
BsiEI	CGRYCG	3 cut(s)	1066, 1603, 1607
BsiHKAI	GWGCWC	2 cut(s)	1613, 1624
BsiHKCI	CYCGRG	1 cut(s)	238
BsiSI	CCGG	3 cut(s)	239, 589, 697
BslFI	GGGAC	3 cut(s)	221, 769, 1514
BslI	CCNNNNNNNGG	4 cut(s)	999, 1237, 1294, 1580
BsmAI	GTCTC	4 cut(s)	22, 47, 1010, 1232
BsmFI	GGGAC	3 cut(s)	221, 769, 1514
BsmI	GAATGC	2 cut(s)	1522, 1524
BsnI	GGCC	4 cut(s)	1280, 1367, 1536, 1569
Bso31I	GGTCTC	1 cut(s)	22
BsoBI	CYCGRG	1 cut(s)	238
Bsp120I	GGGCCC	1 cut(s)	1278
Bsp1286I	GDGCHC	8 cut(s)	140, 406, 583, 832, 953, 1282, 1613, 1624
Bsp13I	TCCGGA	1 cut(s)	588
Bsp143I	GATC	5 cut(s)	591, 1303, 1468, 1600, 1604
BspACI	CCGC	3 cut(s)	623, 940, 1256
BspANI	GGCC	4 cut(s)	1280, 1367, 1536, 1569
BspCNI	CTCAG	4 cut(s)	934, 1070, 1305, 1485
BspDI	ATCGAT	1 cut(s)	1603
BspEI	TCCGGA	1 cut(s)	588
BspHI	TCATGA	1 cut(s)	721
BspLI	GGNNCC	7 cut(s)	9, 363, 593, 831, 991, 1246, 1280
BspMAI	CTGCAG	1 cut(s)	1389
BspPI	GGATC	4 cut(s)	586, 599, 1311, 1476
BspQI	GCTCTTC	1 cut(s)	1386
BspT107I	GGYRCC	1 cut(s)	1244
BspTI	CTTAAG	1 cut(s)	1007
BspTNI	GGTCTC	1 cut(s)	22
BsrDI	GCAATG	1 cut(s)	121
BsrI	ACTGG	4 cut(s)	169, 757, 869, 1115
BssECI	CCNNGG	4 cut(s)	109, 238, 901, 1231
BssMI	GATC	5 cut(s)	591, 1303, 1468, 1600, 1604
BssNI	GRCGYC	1 cut(s)	17
BssT1I	CCWWGG	1 cut(s)	1231
Bst2UI	CCWGG	4 cut(s)	111, 849, 903, 1515
Bst4CI	ACNGT	2 cut(s)	135, 1067
Bst6I	CTCTTC	5 cut(s)	20, 318, 789, 951, 1386
BstACI	GRCGYC	1 cut(s)	17
BstAFI	CTTAAG	1 cut(s)	1007
BstC8I	GCNNGC	2 cut(s)	299, 1571
BstDEI	CTNAG	5 cut(s)	788, 921, 1057, 1313, 1493
BstENI	CCTNNNNNAGG	1 cut(s)	1235
BstKTI	GATC	5 cut(s)	594, 1306, 1471, 1603, 1607
BstMAI	GTCTC	4 cut(s)	22, 47, 1010, 1232
BstMBI	GATC	5 cut(s)	591, 1303, 1468, 1600, 1604
BstMCI	CGRYCG	3 cut(s)	1066, 1603, 1607
BstMWI	GCNNNNNNNGC	2 cut(s)	740, 948
BstNI	CCWGG	4 cut(s)	111, 849, 903, 1515
BstPAI	GACNNNNGTC	1 cut(s)	1065
BstSCI	CCNGG	7 cut(s)	109, 237, 238, 695, 847, 901, 1513
BstSFI	CTRYAG	1 cut(s)	1385
BstSLI	GKGCMC	5 cut(s)	140, 583, 953, 1282, 1624
BstV1I	GCAGC	5 cut(s)	712, 847, 1010, 1185, 1417
BstX2I	RGATCY	1 cut(s)	591
BstYI	RGATCY	1 cut(s)	591
Bsu15I	ATCGAT	1 cut(s)	1603
BsuRI	GGCC	4 cut(s)	1280, 1367, 1536, 1569
BsuTUI	ATCGAT	1 cut(s)	1603
BtgZI	GCGATG	2 cut(s)	465, 648
BtsIMutI	CAGTG	2 cut(s)	162, 750
Cac8I	GCNNGC	2 cut(s)	299, 1571
CciI	TCATGA	1 cut(s)	721
Cfr13I	GGNCC	4 cut(s)	1085, 1278, 1279, 1535
Cfr9I	CCCGGG	1 cut(s)	238
ClaI	ATCGAT	1 cut(s)	1603
CseI	GACGC	4 cut(s)	25, 132, 740, 1433
Csp6I	GTAC	4 cut(s)	131, 354, 1163, 1245
CviAII	CATG	8 cut(s)	35, 347, 377, 618, 722, 887, 1186, 1580
CviQI	GTAC	4 cut(s)	131, 354, 1163, 1245
DdeI	CTNAG	5 cut(s)	788, 921, 1057, 1313, 1493
DpnI	GATC	5 cut(s)	593, 1305, 1470, 1602, 1606
DpnII	GATC	5 cut(s)	591, 1303, 1468, 1600, 1604
Eam1104I	CTCTTC	5 cut(s)	20, 318, 789, 951, 1386
EarI	CTCTTC	5 cut(s)	20, 318, 789, 951, 1386
Eco130I	CCWWGG	1 cut(s)	1231
Eco24I	GRGCYC	3 cut(s)	406, 832, 1282
Eco31I	GGTCTC	1 cut(s)	22
Eco32I	GATATC	1 cut(s)	1110
Eco47I	GGWCC	1 cut(s)	1085
Eco57I	CTGAAG	2 cut(s)	975, 1308
Eco88I	CYCGRG	1 cut(s)	238
EcoNI	CCTNNNNNAGG	1 cut(s)	1235
EcoO109I	RGGNCCY	1 cut(s)	1278
EcoRII	CCWGG	4 cut(s)	109, 847, 901, 1513
EcoRV	GATATC	1 cut(s)	1110
EcoT14I	CCWWGG	1 cut(s)	1231
EcoT22I	ATGCAT	1 cut(s)	1524
EcoT38I	GRGCYC	3 cut(s)	406, 832, 1282
ErhI	CCWWGG	1 cut(s)	1231
FaeI	CATG	8 cut(s)	38, 350, 380, 621, 725, 890, 1189, 1583
FalI	AAGNNNNNCTT	2 cut(s)	1365, 1397
FaqI	GGGAC	3 cut(s)	221, 769, 1514
FatI	CATG	8 cut(s)	34, 346, 376, 617, 721, 886, 1185, 1579
Fnu4HI	GCNGC	6 cut(s)	624, 701, 861, 1024, 1199, 1406
FriOI	GRGCYC	3 cut(s)	406, 832, 1282
Fsp4HI	GCNGC	6 cut(s)	624, 701, 861, 1024, 1199, 1406
FspBI	CTAG	5 cut(s)	357, 503, 641, 1275, 1398
GluI	GCNGC	6 cut(s)	624, 701, 861, 1024, 1199, 1406
GsuI	CTGGAG	2 cut(s)	870, 1497
HaeIII	GGCC	4 cut(s)	1280, 1367, 1536, 1569
HapII	CCGG	3 cut(s)	239, 589, 697
HgaI	GACGC	4 cut(s)	25, 132, 740, 1433
Hin1I	GRCGYC	1 cut(s)	17
Hin1II	CATG	8 cut(s)	38, 350, 380, 621, 725, 890, 1189, 1583
HincII	GTYRAC	1 cut(s)	779
HindII	GTYRAC	1 cut(s)	779
HindIII	AAGCTT	1 cut(s)	93
HinfI	GANTC	1 cut(s)	1055
HpaII	CCGG	3 cut(s)	239, 589, 697
HphI	GGTGA	3 cut(s)	801, 1104, 1500
Hpy166II	GTNNAC	3 cut(s)	779, 874, 1622
Hpy188III	TCNNGA	8 cut(s)	200, 589, 722, 833, 923, 1097, 1421, 1598
Hpy8I	GTNNAC	3 cut(s)	779, 874, 1622
Hpy99I	CGWCG	1 cut(s)	25
HpyAV	CCTTC	8 cut(s)	164, 428, 605, 818, 927, 1037, 1294, 1443
HpyCH4III	ACNGT	2 cut(s)	135, 1067
HpyF10VI	GCNNNNNNNGC	2 cut(s)	740, 948
HpyF3I	CTNAG	5 cut(s)	788, 921, 1057, 1313, 1493
Hsp92I	GRCGYC	1 cut(s)	17
Hsp92II	CATG	8 cut(s)	38, 350, 380, 621, 725, 890, 1189, 1583
Kpn2I	TCCGGA	1 cut(s)	588
KpnI	GGTACC	1 cut(s)	1248
Kzo9I	GATC	5 cut(s)	591, 1303, 1468, 1600, 1604
LguI	GCTCTTC	1 cut(s)	1386
LmnI	GCTCC	4 cut(s)	13, 455, 835, 1616
Lsp1109I	GCAGC	5 cut(s)	712, 847, 1010, 1185, 1417
LweI	GCATC	7 cut(s)	515, 655, 906, 1102, 1114, 1366, 1546
MaeI	CTAG	5 cut(s)	357, 503, 641, 1275, 1398
MaeIII	GTNAC	3 cut(s)	160, 728, 1421
MalI	GATC	5 cut(s)	593, 1305, 1470, 1602, 1606
MboI	GATC	5 cut(s)	591, 1303, 1468, 1600, 1604
MboII	GAAGA	7 cut(s)	37, 335, 476, 776, 968, 1346, 1373
MflI	RGATCY	1 cut(s)	591
MhlI	GDGCHC	8 cut(s)	140, 406, 583, 832, 953, 1282, 1613, 1624
MluCI	AATT	7 cut(s)	222, 766, 881, 975, 1308, 1358, 1528
MlyI	GAGTC	1 cut(s)	1049
Mph1103I	ATGCAT	1 cut(s)	1524
MroI	TCCGGA	1 cut(s)	588
MroXI	GAANNNNTTC	3 cut(s)	642, 1341, 1522
MseI	TTAA	2 cut(s)	1008, 1371
MslI	CAYNNNNRTG	3 cut(s)	726, 891, 1098
MspCI	CTTAAG	1 cut(s)	1007
MspI	CCGG	3 cut(s)	239, 589, 697
MspR9I	CCNGG	7 cut(s)	111, 239, 240, 697, 849, 903, 1515
Mva1269I	GAATGC	2 cut(s)	1522, 1524
MvaI	CCWGG	4 cut(s)	111, 849, 903, 1515
MwoI	GCNNNNNNNGC	2 cut(s)	740, 948
NciI	CCSGG	3 cut(s)	239, 240, 697
NdeII	GATC	5 cut(s)	591, 1303, 1468, 1600, 1604
NlaIII	CATG	8 cut(s)	38, 350, 380, 621, 725, 890, 1189, 1583
NlaIV	GGNNCC	7 cut(s)	9, 363, 593, 831, 991, 1246, 1280
NmuCI	GTSAC	3 cut(s)	160, 728, 1421
NsiI	ATGCAT	1 cut(s)	1524
PagI	TCATGA	1 cut(s)	721
PciSI	GCTCTTC	1 cut(s)	1386
PctI	GAATGC	2 cut(s)	1522, 1524
PdmI	GAANNNNTTC	3 cut(s)	642, 1341, 1522
PflFI	GACNNNGTC	1 cut(s)	19
PfoI	TCCNGGA	1 cut(s)	1513
PkrI	GCNGC	6 cut(s)	625, 702, 862, 1025, 1200, 1407
Ple19I	CGATCG	2 cut(s)	1603, 1607
PleI	GAGTC	1 cut(s)	1049
PpsI	GAGTC	1 cut(s)	1049
PshAI	GACNNNNGTC	1 cut(s)	1065
Psp6I	CCWGG	4 cut(s)	109, 847, 901, 1513
PspGI	CCWGG	4 cut(s)	109, 847, 901, 1513
PspN4I	GGNNCC	7 cut(s)	9, 363, 593, 831, 991, 1246, 1280
PspOMI	GGGCCC	1 cut(s)	1278
PspPI	GGNCC	4 cut(s)	1085, 1278, 1279, 1535
PstI	CTGCAG	1 cut(s)	1389
PsuI	RGATCY	1 cut(s)	591
PsyI	GACNNNGTC	1 cut(s)	19
PvuI	CGATCG	2 cut(s)	1603, 1607
RsaI	GTAC	4 cut(s)	132, 355, 1164, 1246
RsaNI	GTAC	4 cut(s)	131, 354, 1163, 1245
RseI	CAYNNNNRTG	3 cut(s)	726, 891, 1098
SapI	GCTCTTC	1 cut(s)	1386
SaqAI	TTAA	2 cut(s)	1008, 1371
SatI	GCNGC	6 cut(s)	624, 701, 861, 1024, 1199, 1406
Sau3AI	GATC	5 cut(s)	591, 1303, 1468, 1600, 1604
Sau96I	GGNCC	4 cut(s)	1085, 1278, 1279, 1535
SchI	GAGTC	1 cut(s)	1049
ScrFI	CCNGG	7 cut(s)	111, 239, 240, 697, 849, 903, 1515
SduI	GDGCHC	8 cut(s)	140, 406, 583, 832, 953, 1282, 1613, 1624
SfaNI	GCATC	7 cut(s)	515, 655, 906, 1102, 1114, 1366, 1546
SfcI	CTRYAG	1 cut(s)	1385
SinI	GGWCC	1 cut(s)	1085
SmaI	CCCGGG	1 cut(s)	240
SmiMI	CAYNNNNRTG	3 cut(s)	726, 891, 1098
SmlI	CTYRAG	2 cut(s)	516, 1007
SmoI	CTYRAG	2 cut(s)	516, 1007
SpeI	ACTAGT	2 cut(s)	502, 640
Sse9I	AATT	7 cut(s)	222, 766, 881, 975, 1308, 1358, 1528
SsiI	CCGC	3 cut(s)	623, 940, 1256
SspI	AATATT	1 cut(s)	1628
SspMI	CTAG	5 cut(s)	357, 503, 641, 1275, 1398
StyD4I	CCNGG	7 cut(s)	109, 237, 238, 695, 847, 901, 1513
StyI	CCWWGG	1 cut(s)	1231
TaaI	ACNGT	2 cut(s)	135, 1067
TaqI	TCGA	2 cut(s)	23, 1603
TaqII	GACCGA	1 cut(s)	764
TasI	AATT	7 cut(s)	222, 766, 881, 975, 1308, 1358, 1528
TauI	GCSGC	1 cut(s)	626
Tru1I	TTAA	2 cut(s)	1008, 1371
Tru9I	TTAA	2 cut(s)	1008, 1371
TscAI	CASTG	2 cut(s)	169, 757
TseFI	GTSAC	3 cut(s)	160, 728, 1421
TseI	GCWGC	5 cut(s)	700, 860, 1023, 1198, 1405
Tsp45I	GTSAC	3 cut(s)	160, 728, 1421
TspGWI	ACGGA	2 cut(s)	603, 1277
TspMI	CCCGGG	1 cut(s)	238
TspRI	CASTG	2 cut(s)	169, 757
Tth111I	GACNNNGTC	1 cut(s)	19
Vha464I	CTTAAG	1 cut(s)	1007
VneI	GTGCAC	1 cut(s)	1620
VpaK11BI	GGWCC	1 cut(s)	1085
XagI	CCTNNNNNAGG	1 cut(s)	1235
XapI	RAATTY	2 cut(s)	1308, 1358
XmaI	CCCGGG	1 cut(s)	238
XmnI	GAANNNNTTC	3 cut(s)	642, 1341, 1522
XspI	CTAG	5 cut(s)	357, 503, 641, 1275, 1398
Zsp2I	ATGCAT	1 cut(s)	1524

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.