Rorug07G0272600

KH domain-containing protein At4g18375-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0010574
Physical Location & Seq
Reverse (-)
25831081 .. 25832107
1027 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0272600.1

Sequence Viewer

Length: 888 bp
ATGTGTGATGGGCCTGGATTGATCCAGTTCTGGAACGCAGTAGGGGAGATGGTTCAAGGAAAAAGTGCACCATCTATTCCACCAGTGTGGGAGCGAGAGGTCTTGAATGCCCGAGATCCACCACGAATTACATGTACACATCACGAATACGAGGAAATTATTGATGAATACTCAGATAAGGGCTCTGATGCGGCAGTAATGGTGCAAAAATCTTTCTACTTTGGTCCCAATGAGATAAGGGCCTTGAAGAAACATCTTCCACCACACCTTTCCAATTGCTCTACATTCGACCTTATAAGAACGTGTTTGTGGAAGTGCCGCACAGTTGCACTAGAACTTAATCCGGAACAGGTTGTTCGGGTTTCGTGCATAGTCAATGCACGTGGGAAGCGCAACAGTCTAGGTCTTCCTTTGGGTTACTACGGCAATGCATTCGCATACCCAGCTGCTGTTTCGGGAGTGAAACAATTGTGTGAAAGTCCGTTGGGATATGCACTGGAGTTGGTGATGAAGGCAAAAAATGAAATGAATGAAGAGTACATGAGATCAGTGGCAGATCTTATGGTCATAAGAGGACGGCCTCCATATACATTGACCGGGAACTTCACAGTTTCAGATAATAGACTTACTCGCATTGGACATGTCAACTTGGGGTTGGGAGAGCCAACGTTTTCTGGACCTGCCAAGGCCTTGAAGTTCCTTAGTTTCTATATTCAGCACAAAAAGGAAAGAGAGGACGGGATTTTGGTGCCAGTGTGCTTGCCATCGTGGAGTATGGCGAGATTTCAGCAGGAACTCGAGAAGATGACGTCGTCGGAGATAGTGGGAAATATAAATAACACTAAATCTGCTAATATTAATGTTGCAAGGATGATTCCACGGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1

Protein Analysis

295

Amino Acids

33.02

Weight (kDa)

8.56

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 1 - 269 8.1e-29 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 296
AatII GACGTC 1 cut(s) 812
Acc36I ACCTGC 1 cut(s) 688
AccB1I GGYRCC 1 cut(s) 748
AccIII TCCGGA 1 cut(s) 343
AciI CCGC 2 cut(s) 191, 319
AclI AACGTT 1 cut(s) 668
AclWI GGATC 2 cut(s) 16, 110
AcvI CACGTG 1 cut(s) 383
AcyI GRCGYC 1 cut(s) 809
AfaI GTAC 2 cut(s) 136, 539
AflIII ACRYGT 3 cut(s) 131, 302, 640
AgsI TTSAA 4 cut(s) 56, 106, 247, 694
AjnI CCWGG 1 cut(s) 13
AleI CACNNNNGTG 1 cut(s) 85
AluBI AGCT 1 cut(s) 446
AluI AGCT 1 cut(s) 446
Alw21I GWGCWC 1 cut(s) 70
Alw44I GTGCAC 1 cut(s) 66
AlwI GGATC 2 cut(s) 16, 110
AlwNI CAGNNNCTG 1 cut(s) 449
Ama87I CYCGRG 2 cut(s) 111, 797
Aor13HI TCCGGA 1 cut(s) 343
AoxI GGCC 4 cut(s) 11, 240, 578, 687
ApaLI GTGCAC 1 cut(s) 66
ApeKI GCWGC 1 cut(s) 446
AseI ATTAAT 1 cut(s) 858
AspLEI GCGC 1 cut(s) 393
AspS9I GGNCC 4 cut(s) 11, 224, 240, 677
AsuC2I CCSGG 1 cut(s) 598
AsuHPI GGTGA 1 cut(s) 517
AvaI CYCGRG 2 cut(s) 111, 797
AvaII GGWCC 2 cut(s) 224, 677
BaeGI GKGCMC 1 cut(s) 70
BanI GGYRCC 1 cut(s) 748
BanII GRGCYC 1 cut(s) 185
BbrPI CACGTG 1 cut(s) 383
BbsI GAAGAC 1 cut(s) 398
Bbv12I GWGCWC 1 cut(s) 70
BbvI GCAGC 1 cut(s) 433
BccI CCATC 4 cut(s) 2, 43, 79, 772
BceAI ACGGC 2 cut(s) 439, 593
BcgI CGANNNNNNTGC 2 cut(s) 415, 449
BciT130I CCWGG 1 cut(s) 15
BcnI CCSGG 1 cut(s) 598
BfaI CTAG 2 cut(s) 332, 401
BfuAI ACCTGC 1 cut(s) 688
BglII AGATCT 1 cut(s) 556
BisI GCNGC 3 cut(s) 192, 319, 447
BlsI GCNGC 3 cut(s) 193, 320, 448
Bme1390I CCNGG 2 cut(s) 15, 598
Bme18I GGWCC 2 cut(s) 224, 677
BmeT110I CYCGRG 2 cut(s) 111, 797
BmgT120I GGNCC 4 cut(s) 11, 224, 240, 677
BmiI GGNNCC 2 cut(s) 226, 750
BmrFI CCNGG 2 cut(s) 15, 598
BmsI GCATC 1 cut(s) 178
BpiI GAAGAC 1 cut(s) 398
BpmI CTGGAG 1 cut(s) 518
BpuMI CCSGG 1 cut(s) 598
BsaAI YACGTR 1 cut(s) 383
BsaHI GRCGYC 1 cut(s) 809
BsaJI CCNNGG 2 cut(s) 684, 878
BsaWI WCCGGW 1 cut(s) 343
Bse1I ACTGG 4 cut(s) 25, 83, 501, 752
Bse3DI GCAATG 1 cut(s) 433
BseAI TCCGGA 1 cut(s) 343
BseBI CCWGG 1 cut(s) 15
BseDI CCNNGG 2 cut(s) 684, 878
BseGI GGATG 1 cut(s) 876
BseMI GCAATG 1 cut(s) 433
BseMII CTCAG 1 cut(s) 186
BseNI ACTGG 4 cut(s) 25, 83, 501, 752
BseSI GKGCMC 1 cut(s) 70
BseXI GCAGC 1 cut(s) 433
BseYI CCCAGC 1 cut(s) 442
BshFI GGCC 4 cut(s) 13, 242, 580, 689
BshNI GGYRCC 1 cut(s) 748
BsiHKAI GWGCWC 1 cut(s) 70
BsiHKCI CYCGRG 2 cut(s) 111, 797
BsiSI CCGG 2 cut(s) 344, 597
BslFI GGGAC 1 cut(s) 210
BsmFI GGGAC 1 cut(s) 210
BsmI GAATGC 2 cut(s) 112, 431
BsnI GGCC 4 cut(s) 13, 242, 580, 689
BsoBI CYCGRG 2 cut(s) 111, 797
Bsp1286I GDGCHC 2 cut(s) 70, 185
Bsp13I TCCGGA 1 cut(s) 343
Bsp1407I TGTACA 1 cut(s) 134
Bsp143I GATC 4 cut(s) 21, 115, 545, 556
BspACI CCGC 2 cut(s) 191, 319
BspANI GGCC 4 cut(s) 13, 242, 580, 689
BspCNI CTCAG 1 cut(s) 185
BspEI TCCGGA 1 cut(s) 343
BspLI GGNNCC 2 cut(s) 226, 750
BspMI ACCTGC 1 cut(s) 688
BspPI GGATC 2 cut(s) 16, 110
BspT107I GGYRCC 1 cut(s) 748
BsrDI GCAATG 1 cut(s) 433
BsrGI TGTACA 1 cut(s) 134
BsrI ACTGG 4 cut(s) 25, 83, 501, 752
BssECI CCNNGG 2 cut(s) 684, 878
BssMI GATC 4 cut(s) 21, 115, 545, 556
BssNI GRCGYC 1 cut(s) 809
BssT1I CCWWGG 1 cut(s) 684
Bst2UI CCWGG 1 cut(s) 15
Bst4CI ACNGT 4 cut(s) 325, 398, 610, 882
Bst6I CTCTTC 1 cut(s) 528
BstACI GRCGYC 1 cut(s) 809
BstAUI TGTACA 1 cut(s) 134
BstBAI YACGTR 1 cut(s) 383
BstC8I GCNNGC 1 cut(s) 761
BstDEI CTNAG 2 cut(s) 172, 701
BstDSI CCRYGG 1 cut(s) 878
BstF5I GGATG 1 cut(s) 876
BstHHI GCGC 1 cut(s) 393
BstKTI GATC 4 cut(s) 24, 118, 548, 559
BstMBI GATC 4 cut(s) 21, 115, 545, 556
BstMWI GCNNNNNNNGC 1 cut(s) 443
BstNI CCWGG 1 cut(s) 15
BstNSI RCATGY 2 cut(s) 135, 644
BstSCI CCNGG 2 cut(s) 13, 596
BstSLI GKGCMC 1 cut(s) 70
BstV1I GCAGC 1 cut(s) 433
BstV2I GAAGAC 1 cut(s) 398
BstX2I RGATCY 2 cut(s) 115, 556
BstXI CCANNNNNNTGG 1 cut(s) 87
BstYI RGATCY 2 cut(s) 115, 556
BsuRI GGCC 4 cut(s) 13, 242, 580, 689
BtgI CCRYGG 1 cut(s) 878
BtsCI GGATG 1 cut(s) 876
BtsIMutI CAGTG 4 cut(s) 90, 494, 555, 759
BveI ACCTGC 1 cut(s) 688
Cac8I GCNNGC 1 cut(s) 761
CaiI CAGNNNCTG 1 cut(s) 449
CfoI GCGC 1 cut(s) 393
Cfr13I GGNCC 4 cut(s) 11, 224, 240, 677
Csp6I GTAC 2 cut(s) 135, 538
CspCI CAANNNNNGTGG 2 cut(s) 364, 399
CviAII CATG 3 cut(s) 132, 541, 641
CviJI RGCY 7 cut(s) 13, 183, 242, 446, 580, 664, 689
CviKI_1 RGCY 7 cut(s) 13, 183, 242, 446, 580, 664, 689
CviQI GTAC 2 cut(s) 135, 538
DdeI CTNAG 2 cut(s) 172, 701
DpnI GATC 4 cut(s) 23, 117, 547, 558
DpnII GATC 4 cut(s) 21, 115, 545, 556
Eam1104I CTCTTC 1 cut(s) 528
EarI CTCTTC 1 cut(s) 528
Eco130I CCWWGG 1 cut(s) 684
Eco147I AGGCCT 1 cut(s) 689
Eco24I GRGCYC 1 cut(s) 185
Eco47I GGWCC 2 cut(s) 224, 677
Eco72I CACGTG 1 cut(s) 383
Eco88I CYCGRG 2 cut(s) 111, 797
EcoO109I RGGNCCY 1 cut(s) 240
EcoRII CCWGG 1 cut(s) 13
EcoT14I CCWWGG 1 cut(s) 684
EcoT22I ATGCAT 1 cut(s) 433
EcoT38I GRGCYC 1 cut(s) 185
ErhI CCWWGG 1 cut(s) 684
FaeI CATG 3 cut(s) 135, 544, 644
FaqI GGGAC 1 cut(s) 210
FatI CATG 3 cut(s) 131, 540, 640
Fnu4HI GCNGC 3 cut(s) 192, 319, 447
FokI GGATG 1 cut(s) 883
FriOI GRGCYC 1 cut(s) 185
Fsp4HI GCNGC 3 cut(s) 192, 319, 447
FspBI CTAG 2 cut(s) 332, 401
GlaI GCGC 1 cut(s) 392
GluI GCNGC 3 cut(s) 192, 319, 447
GsaI CCCAGC 1 cut(s) 446
GsuI CTGGAG 1 cut(s) 518
HaeIII GGCC 4 cut(s) 13, 242, 580, 689
HapII CCGG 2 cut(s) 344, 597
HhaI GCGC 1 cut(s) 393
Hin1I GRCGYC 1 cut(s) 809
Hin1II CATG 3 cut(s) 135, 544, 644
Hin6I GCGC 1 cut(s) 391
HinP1I GCGC 1 cut(s) 391
HincII GTYRAC 1 cut(s) 646
HindII GTYRAC 1 cut(s) 646
HinfI GANTC 1 cut(s) 874
HpaII CCGG 2 cut(s) 344, 597
HphI GGTGA 1 cut(s) 517
Hpy166II GTNNAC 3 cut(s) 68, 137, 646
Hpy188I TCNGA 4 cut(s) 175, 187, 616, 817
Hpy188III TCNNGA 7 cut(s) 31, 103, 143, 344, 456, 675, 799
Hpy8I GTNNAC 3 cut(s) 68, 137, 646
Hpy99I CGWCG 2 cut(s) 814, 817
HpyAV CCTTC 1 cut(s) 505
HpyCH4III ACNGT 4 cut(s) 325, 398, 610, 882
HpyCH4IV ACGT 4 cut(s) 302, 382, 668, 809
HpyCH4V TGCA 8 cut(s) 68, 205, 329, 369, 380, 431, 494, 866
HpyF10VI GCNNNNNNNGC 1 cut(s) 443
HpyF3I CTNAG 2 cut(s) 172, 701
HpySE526I ACGT 4 cut(s) 302, 382, 668, 809
Hsp92I GRCGYC 1 cut(s) 809
Hsp92II CATG 3 cut(s) 135, 544, 644
HspAI GCGC 1 cut(s) 391
Kpn2I TCCGGA 1 cut(s) 343
Kzo9I GATC 4 cut(s) 21, 115, 545, 556
LmnI GCTCC 1 cut(s) 91
Lsp1109I GCAGC 1 cut(s) 433
LweI GCATC 1 cut(s) 178
MaeI CTAG 2 cut(s) 332, 401
MaeII ACGT 4 cut(s) 302, 382, 668, 809
MaeIII GTNAC 1 cut(s) 416
MalI GATC 4 cut(s) 23, 117, 547, 558
MboI GATC 4 cut(s) 21, 115, 545, 556
MboII GAAGA 5 cut(s) 248, 259, 398, 545, 814
MfeI CAATTG 2 cut(s) 274, 467
MflI RGATCY 2 cut(s) 115, 556
MhlI GDGCHC 2 cut(s) 70, 185
MluCI AATT 4 cut(s) 126, 156, 274, 467
MmeI TCCRAC 1 cut(s) 795
MnlI CCTC 5 cut(s) 91, 145, 566, 591, 727
Mph1103I ATGCAT 1 cut(s) 433
MroI TCCGGA 1 cut(s) 343
MseI TTAA 2 cut(s) 339, 858
MslI CAYNNNNRTG 1 cut(s) 85
MspA1I CMGCKG 1 cut(s) 446
MspI CCGG 2 cut(s) 344, 597
MspR9I CCNGG 2 cut(s) 15, 598
MunI CAATTG 2 cut(s) 274, 467
Mva1269I GAATGC 2 cut(s) 112, 431
MvaI CCWGG 1 cut(s) 15
MwoI GCNNNNNNNGC 1 cut(s) 443
NciI CCSGG 1 cut(s) 598
NdeII GATC 4 cut(s) 21, 115, 545, 556
NlaIII CATG 3 cut(s) 135, 544, 644
NlaIV GGNNCC 2 cut(s) 226, 750
NsiI ATGCAT 1 cut(s) 433
NspI RCATGY 2 cut(s) 135, 644
OliI CACNNNNGTG 1 cut(s) 85
PaeR7I CTCGAG 1 cut(s) 797
PceI AGGCCT 1 cut(s) 689
PciI ACATGT 2 cut(s) 131, 640
PctI GAATGC 2 cut(s) 112, 431
PfeI GAWTC 1 cut(s) 874
PflFI GACNNNGTC 1 cut(s) 811
PkrI GCNGC 3 cut(s) 193, 320, 448
PmaCI CACGTG 1 cut(s) 383
PmlI CACGTG 1 cut(s) 383
Ppu21I YACGTR 1 cut(s) 383
PscI ACATGT 2 cut(s) 131, 640
PshBI ATTAAT 1 cut(s) 858
PsiI TTATAA 1 cut(s) 296
Psp1406I AACGTT 1 cut(s) 668
Psp6I CCWGG 1 cut(s) 13
PspCI CACGTG 1 cut(s) 383
PspFI CCCAGC 1 cut(s) 442
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 2 cut(s) 226, 750
PspPI GGNCC 4 cut(s) 11, 224, 240, 677
PstNI CAGNNNCTG 1 cut(s) 449
PsuI RGATCY 2 cut(s) 115, 556
PsyI GACNNNGTC 1 cut(s) 811
PvuII CAGCTG 1 cut(s) 446
RsaI GTAC 2 cut(s) 136, 539
RsaNI GTAC 2 cut(s) 135, 538
RseI CAYNNNNRTG 1 cut(s) 85
SaqAI TTAA 2 cut(s) 339, 858
SatI GCNGC 3 cut(s) 192, 319, 447
Sau3AI GATC 4 cut(s) 21, 115, 545, 556
Sau96I GGNCC 4 cut(s) 11, 224, 240, 677
ScrFI CCNGG 2 cut(s) 15, 598
SduI GDGCHC 2 cut(s) 70, 185
SfaNI GCATC 1 cut(s) 178
Sfr274I CTCGAG 1 cut(s) 797
SinI GGWCC 2 cut(s) 224, 677
SlaI CTCGAG 1 cut(s) 797
SmiMI CAYNNNNRTG 1 cut(s) 85
SmlI CTYRAG 1 cut(s) 797
SmoI CTYRAG 1 cut(s) 797
Sse9I AATT 4 cut(s) 126, 156, 274, 467
SseBI AGGCCT 1 cut(s) 689
SsiI CCGC 2 cut(s) 191, 319
SspI AATATT 1 cut(s) 856
SspMI CTAG 2 cut(s) 332, 401
StuI AGGCCT 1 cut(s) 689
StyD4I CCNGG 2 cut(s) 13, 596
StyI CCWWGG 1 cut(s) 684
TaaI ACNGT 4 cut(s) 325, 398, 610, 882
TaiI ACGT 4 cut(s) 305, 385, 671, 812
TaqI TCGA 2 cut(s) 288, 798
TasI AATT 4 cut(s) 126, 156, 274, 467
TatI WGTACW 2 cut(s) 134, 537
TauI GCSGC 2 cut(s) 194, 321
TfiI GAWTC 1 cut(s) 874
Tru1I TTAA 2 cut(s) 339, 858
Tru9I TTAA 2 cut(s) 339, 858
TscAI CASTG 4 cut(s) 90, 501, 555, 759
TseI GCWGC 1 cut(s) 446
TspDTI ATGAA 5 cut(s) 180, 524, 537, 542, 546
TspGWI ACGGA 1 cut(s) 471
TspRI CASTG 4 cut(s) 90, 501, 555, 759
Tth111I GACNNNGTC 1 cut(s) 811
VneI GTGCAC 1 cut(s) 66
VpaK11BI GGWCC 2 cut(s) 224, 677
VspI ATTAAT 1 cut(s) 858
XceI RCATGY 2 cut(s) 135, 644
XhoI CTCGAG 1 cut(s) 797
XspI CTAG 2 cut(s) 332, 401
ZraI GACGTC 1 cut(s) 810
Zsp2I ATGCAT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.