Rorug07G0272500

KH domain-containing protein At4g18375-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0010574
Physical Location & Seq
Reverse (-)
25819635 .. 25820989
1355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0272500.1

Sequence Viewer

Length: 933 bp
ATGGCGTTGCCATGCTCCTTAGTATTTCAGGTTAATAGAACCCAGCCACAACTTATAACTCCAGCAAGGCCAACTCCTCGTGAAACAAAGATGCTGTCAGATATAGATGGCCAGCAAGGCCTTAGGTTTCAGGCACCAGTCATTGCAGCCTTCAAGAACAATCCTTCAATGTCGAACCGAAGAGACCCTGTTCAGGTGATCAGGGAAGCAATAAGTAGAGCATTGGTGTATTACTACCCTTTGGCAGGTAGGCTCAGGGAAGGGCCTAACAGAAAGCTTTTGGTGGATTGCAACGGAGAAGGTGTCTTGTTCGTTGGGGCTAATGCTGACGTTACTCTCGAGGAAATTGGAGATGCTATTCTACCCCCTTGCCCTGTGTTAGAGGACTTCCTATGTAATGTCCTGGGCTCGGATGGCATTCTTGGTTGCCCTTTGTTGTTAGTTCAGGTGACCATTTTGAGATGTGGAGGTTTCATACTTGCATTGCGCGTGAACCACACAATTTGTGATGCGCCTGGATTTGTCCTGTTCTTGAACACGGTTGGGGAGATGGTTCAAGGAAAAAGTGCACCATCTATTCCACCAGTGTGGGAGCGAGAGGTCTTGAATGCCCGAGATCCACCACGAATTACATGTACACATCACGAATACGAGGAAATTATTGATGAATACTCAGATAAGGGCTCTGATGCGGCAGTAATGGTGCAAAAATCTTTCTACTTTGGTCCCAATGAGATAAGGGCCTTGAAGAAACATCTTCCACCACACCTTTCCAATTGCTCTACATTCGACCTTATAACATCGTGTTTGTGGAAGTGCCGCACAGTTGCACTAGAACTTAATCCGGAACAGGATTACCCGCTACACCGTTTGAATAGTGTACCGGGATTGCAACAATACAAACCCGGTAAGCTTTTTGCAAAGCTCGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1

Protein Analysis

310

Amino Acids

34.39

Weight (kDa)

6.6

Isoelectric Point (pI)

41.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 10 - 285 2.3e-54 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 56, 797
Acc36I ACCTGC 1 cut(s) 236
AccB1I GGYRCC 1 cut(s) 133
AccII CGCG 1 cut(s) 489
AccIII TCCGGA 1 cut(s) 844
AciI CCGC 3 cut(s) 692, 820, 860
AclWI GGATC 1 cut(s) 611
AcoI YGGCCR 1 cut(s) 109
AfaI GTAC 2 cut(s) 637, 882
AfiI CCNNNNNNNGG 3 cut(s) 193, 245, 409
AflIII ACRYGT 1 cut(s) 632
AgsI TTSAA 7 cut(s) 154, 168, 535, 557, 607, 748, 874
AjnI CCWGG 2 cut(s) 402, 514
AleI CACNNNNGTG 1 cut(s) 586
AluBI AGCT 3 cut(s) 277, 913, 925
AluI AGCT 3 cut(s) 277, 913, 925
Alw21I GWGCWC 1 cut(s) 571
Alw26I GTCTC 1 cut(s) 177
Alw44I GTGCAC 1 cut(s) 567
AlwI GGATC 1 cut(s) 611
Ama87I CYCGRG 2 cut(s) 338, 612
Aor13HI TCCGGA 1 cut(s) 844
AoxI GGCC 5 cut(s) 68, 109, 118, 263, 741
ApaLI GTGCAC 1 cut(s) 567
ApeKI GCWGC 1 cut(s) 146
ArsI GACNNNNNNTTYG 2 cut(s) 80, 112
AspLEI GCGC 2 cut(s) 489, 514
AspS9I GGNCC 3 cut(s) 263, 725, 741
AsuC2I CCSGG 2 cut(s) 885, 906
AsuHPI GGTGA 2 cut(s) 208, 460
AvaI CYCGRG 2 cut(s) 338, 612
AvaII GGWCC 1 cut(s) 725
AxyI CCTNAGG 1 cut(s) 122
BaeGI GKGCMC 1 cut(s) 571
BalI TGGCCA 1 cut(s) 111
BanI GGYRCC 1 cut(s) 133
BanII GRGCYC 2 cut(s) 410, 686
BauI CACGAG 1 cut(s) 78
Bbv12I GWGCWC 1 cut(s) 571
BbvI GCAGC 1 cut(s) 158
BccI CCATC 4 cut(s) 101, 407, 544, 580
BciT130I CCWGG 2 cut(s) 404, 516
BclI TGATCA 1 cut(s) 198
BcnI CCSGG 2 cut(s) 885, 906
BcoDI GTCTC 1 cut(s) 177
BfaI CTAG 1 cut(s) 833
BfuAI ACCTGC 1 cut(s) 236
BglI GCCNNNNNGGC 1 cut(s) 117
BisI GCNGC 3 cut(s) 147, 693, 820
BlsI GCNGC 3 cut(s) 148, 694, 821
Bme1390I CCNGG 4 cut(s) 404, 516, 885, 906
Bme18I GGWCC 1 cut(s) 725
BmeT110I CYCGRG 2 cut(s) 338, 612
BmgT120I GGNCC 3 cut(s) 263, 725, 741
BmiI GGNNCC 2 cut(s) 135, 727
BmrFI CCNGG 4 cut(s) 404, 516, 885, 906
BmsI GCATC 4 cut(s) 81, 343, 499, 679
BpmI CTGGAG 1 cut(s) 45
Bpu10I CCTNAGC 1 cut(s) 254
BpuMI CCSGG 2 cut(s) 885, 906
BsaI GGTCTC 1 cut(s) 177
BsaJI CCNNGG 1 cut(s) 403
BsaWI WCCGGW 1 cut(s) 844
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 245, 409
Bse1I ACTGG 2 cut(s) 137, 584
Bse21I CCTNAGG 1 cut(s) 122
Bse3DI GCAATG 2 cut(s) 141, 482
BseAI TCCGGA 1 cut(s) 844
BseBI CCWGG 2 cut(s) 404, 516
BseDI CCNNGG 1 cut(s) 403
BseGI GGATG 1 cut(s) 418
BseLI CCNNNNNNNGG 3 cut(s) 193, 245, 409
BseMI GCAATG 2 cut(s) 141, 482
BseMII CTCAG 2 cut(s) 268, 687
BseNI ACTGG 2 cut(s) 137, 584
BseRI GAGGAG 1 cut(s) 66
BseSI GKGCMC 1 cut(s) 571
BseXI GCAGC 1 cut(s) 158
BseYI CCCAGC 1 cut(s) 42
Bsh1236I CGCG 1 cut(s) 489
BshFI GGCC 5 cut(s) 70, 111, 120, 265, 743
BshNI GGYRCC 1 cut(s) 133
BsiHKAI GWGCWC 1 cut(s) 571
BsiHKCI CYCGRG 2 cut(s) 338, 612
BsiSI CCGG 3 cut(s) 845, 884, 906
BslFI GGGAC 1 cut(s) 711
BslI CCNNNNNNNGG 3 cut(s) 193, 245, 409
BsmAI GTCTC 1 cut(s) 177
BsmFI GGGAC 1 cut(s) 711
BsmI GAATGC 2 cut(s) 417, 613
BsnI GGCC 5 cut(s) 70, 111, 120, 265, 743
Bso31I GGTCTC 1 cut(s) 177
BsoBI CYCGRG 2 cut(s) 338, 612
Bsp1286I GDGCHC 3 cut(s) 410, 571, 686
Bsp13I TCCGGA 1 cut(s) 844
Bsp1407I TGTACA 1 cut(s) 635
Bsp143I GATC 2 cut(s) 198, 616
BspACI CCGC 3 cut(s) 692, 820, 860
BspANI GGCC 5 cut(s) 70, 111, 120, 265, 743
BspCNI CTCAG 2 cut(s) 267, 686
BspEI TCCGGA 1 cut(s) 844
BspFNI CGCG 1 cut(s) 489
BspLI GGNNCC 2 cut(s) 135, 727
BspMI ACCTGC 1 cut(s) 236
BspPI GGATC 1 cut(s) 611
BspT107I GGYRCC 1 cut(s) 133
BspTNI GGTCTC 1 cut(s) 177
BsrDI GCAATG 2 cut(s) 141, 482
BsrGI TGTACA 1 cut(s) 635
BsrI ACTGG 2 cut(s) 137, 584
BssECI CCNNGG 1 cut(s) 403
BssMI GATC 2 cut(s) 198, 616
BssSI CACGAG 1 cut(s) 78
Bst2BI CACGAG 1 cut(s) 78
Bst2UI CCWGG 2 cut(s) 404, 516
Bst4CI ACNGT 3 cut(s) 541, 826, 869
Bst6I CTCTTC 1 cut(s) 175
BstAUI TGTACA 1 cut(s) 635
BstC8I GCNNGC 1 cut(s) 113
BstDEI CTNAG 4 cut(s) 19, 122, 254, 673
BstEII GGTNACC 1 cut(s) 448
BstENI CCTNNNNNAGG 1 cut(s) 243
BstF5I GGATG 1 cut(s) 418
BstFNI CGCG 1 cut(s) 489
BstHHI GCGC 2 cut(s) 489, 514
BstKTI GATC 2 cut(s) 201, 619
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 2 cut(s) 198, 616
BstMWI GCNNNNNNNGC 2 cut(s) 117, 414
BstNI CCWGG 2 cut(s) 404, 516
BstNSI RCATGY 1 cut(s) 636
BstPI GGTNACC 1 cut(s) 448
BstSCI CCNGG 4 cut(s) 402, 514, 883, 904
BstSLI GKGCMC 1 cut(s) 571
BstUI CGCG 1 cut(s) 489
BstV1I GCAGC 1 cut(s) 158
BstX2I RGATCY 1 cut(s) 616
BstXI CCANNNNNNTGG 1 cut(s) 588
BstYI RGATCY 1 cut(s) 616
Bsu36I CCTNAGG 1 cut(s) 122
BsuRI GGCC 5 cut(s) 70, 111, 120, 265, 743
BtsCI GGATG 1 cut(s) 418
BtsIMutI CAGTG 1 cut(s) 591
BveI ACCTGC 1 cut(s) 236
Cac8I GCNNGC 1 cut(s) 113
CfoI GCGC 2 cut(s) 489, 514
Cfr13I GGNCC 3 cut(s) 263, 725, 741
Csp6I GTAC 2 cut(s) 636, 881
CspCI CAANNNNNGTGG 2 cut(s) 485, 520
CviAII CATG 2 cut(s) 12, 633
CviQI GTAC 2 cut(s) 636, 881
DdeI CTNAG 4 cut(s) 19, 122, 254, 673
DpnI GATC 2 cut(s) 200, 618
DpnII GATC 2 cut(s) 198, 616
EaeI YGGCCR 1 cut(s) 109
Eam1104I CTCTTC 1 cut(s) 175
EarI CTCTTC 1 cut(s) 175
Eco147I AGGCCT 1 cut(s) 120
Eco24I GRGCYC 2 cut(s) 410, 686
Eco31I GGTCTC 1 cut(s) 177
Eco47I GGWCC 1 cut(s) 725
Eco81I CCTNAGG 1 cut(s) 122
Eco88I CYCGRG 2 cut(s) 338, 612
Eco91I GGTNACC 1 cut(s) 448
EcoNI CCTNNNNNAGG 1 cut(s) 243
EcoO109I RGGNCCY 2 cut(s) 263, 741
EcoO65I GGTNACC 1 cut(s) 448
EcoRII CCWGG 2 cut(s) 402, 514
EcoT38I GRGCYC 2 cut(s) 410, 686
FaeI CATG 2 cut(s) 15, 636
FaiI YATR 8 cut(s) 13, 56, 104, 394, 476, 634, 797, 931
FaqI GGGAC 1 cut(s) 711
FatI CATG 2 cut(s) 11, 632
FauI CCCGC 1 cut(s) 867
FbaI TGATCA 1 cut(s) 198
Fnu4HI GCNGC 3 cut(s) 147, 693, 820
FokI GGATG 1 cut(s) 425
FriOI GRGCYC 2 cut(s) 410, 686
Fsp4HI GCNGC 3 cut(s) 147, 693, 820
FspBI CTAG 1 cut(s) 833
GlaI GCGC 2 cut(s) 488, 513
GluI GCNGC 3 cut(s) 147, 693, 820
GsaI CCCAGC 1 cut(s) 46
GsuI CTGGAG 1 cut(s) 45
HaeIII GGCC 5 cut(s) 70, 111, 120, 265, 743
HapII CCGG 3 cut(s) 845, 884, 906
HhaI GCGC 2 cut(s) 489, 514
Hin1II CATG 2 cut(s) 15, 636
Hin6I GCGC 2 cut(s) 487, 512
HinP1I GCGC 2 cut(s) 487, 512
HindIII AAGCTT 2 cut(s) 275, 911
HpaII CCGG 3 cut(s) 845, 884, 906
HphI GGTGA 2 cut(s) 208, 460
Hpy166II GTNNAC 4 cut(s) 493, 569, 638, 881
Hpy188I TCNGA 4 cut(s) 100, 412, 676, 688
Hpy188III TCNNGA 7 cut(s) 80, 154, 338, 532, 604, 644, 845
Hpy8I GTNNAC 4 cut(s) 493, 569, 638, 881
HpyAV CCTTC 4 cut(s) 160, 174, 254, 293
HpyCH4III ACNGT 3 cut(s) 541, 826, 869
HpyCH4IV ACGT 1 cut(s) 330
HpyCH4V TGCA 8 cut(s) 146, 291, 482, 569, 706, 830, 892, 920
HpyF10VI GCNNNNNNNGC 2 cut(s) 117, 414
HpyF3I CTNAG 4 cut(s) 19, 122, 254, 673
HpySE526I ACGT 1 cut(s) 330
Hsp92II CATG 2 cut(s) 15, 636
HspAI GCGC 2 cut(s) 487, 512
Kpn2I TCCGGA 1 cut(s) 844
Ksp22I TGATCA 1 cut(s) 198
Kzo9I GATC 2 cut(s) 198, 616
LmnI GCTCC 2 cut(s) 20, 592
Lsp1109I GCAGC 1 cut(s) 158
LweI GCATC 4 cut(s) 81, 343, 499, 679
MaeI CTAG 1 cut(s) 833
MaeII ACGT 1 cut(s) 330
MaeIII GTNAC 2 cut(s) 331, 448
MalI GATC 2 cut(s) 200, 618
MboI GATC 2 cut(s) 198, 616
MboII GAAGA 3 cut(s) 192, 749, 760
MfeI CAATTG 1 cut(s) 775
MflI RGATCY 1 cut(s) 616
MhlI GDGCHC 3 cut(s) 410, 571, 686
MlsI TGGCCA 1 cut(s) 111
MluCI AATT 5 cut(s) 345, 501, 627, 657, 775
MluNI TGGCCA 1 cut(s) 111
MnlI CCTC 6 cut(s) 87, 334, 376, 461, 592, 646
Mox20I TGGCCA 1 cut(s) 111
MroI TCCGGA 1 cut(s) 844
MscI TGGCCA 1 cut(s) 111
MseI TTAA 2 cut(s) 33, 840
MslI CAYNNNNRTG 1 cut(s) 586
Msp20I TGGCCA 1 cut(s) 111
MspI CCGG 3 cut(s) 845, 884, 906
MspR9I CCNGG 4 cut(s) 404, 516, 885, 906
MunI CAATTG 1 cut(s) 775
Mva1269I GAATGC 2 cut(s) 417, 613
MvaI CCWGG 2 cut(s) 404, 516
MvnI CGCG 1 cut(s) 489
MwoI GCNNNNNNNGC 2 cut(s) 117, 414
NciI CCSGG 2 cut(s) 885, 906
NdeII GATC 2 cut(s) 198, 616
NlaIII CATG 2 cut(s) 15, 636
NlaIV GGNNCC 2 cut(s) 135, 727
NmuCI GTSAC 1 cut(s) 448
NspI RCATGY 1 cut(s) 636
OliI CACNNNNGTG 1 cut(s) 586
PaeR7I CTCGAG 1 cut(s) 338
PceI AGGCCT 1 cut(s) 120
PciI ACATGT 1 cut(s) 632
PcsI WCGNNNNNNNCGW 1 cut(s) 336
PctI GAATGC 2 cut(s) 417, 613
PkrI GCNGC 3 cut(s) 148, 694, 821
PscI ACATGT 1 cut(s) 632
PsiI TTATAA 2 cut(s) 56, 797
Psp6I CCWGG 2 cut(s) 402, 514
PspEI GGTNACC 1 cut(s) 448
PspFI CCCAGC 1 cut(s) 42
PspGI CCWGG 2 cut(s) 402, 514
PspN4I GGNNCC 2 cut(s) 135, 727
PspPI GGNCC 3 cut(s) 263, 725, 741
PsuI RGATCY 1 cut(s) 616
RsaI GTAC 2 cut(s) 637, 882
RsaNI GTAC 2 cut(s) 636, 881
RseI CAYNNNNRTG 1 cut(s) 586
SaqAI TTAA 2 cut(s) 33, 840
SatI GCNGC 3 cut(s) 147, 693, 820
Sau3AI GATC 2 cut(s) 198, 616
Sau96I GGNCC 3 cut(s) 263, 725, 741
ScrFI CCNGG 4 cut(s) 404, 516, 885, 906
SduI GDGCHC 3 cut(s) 410, 571, 686
SfaNI GCATC 4 cut(s) 81, 343, 499, 679
SfiI GGCCNNNNNGGCC 1 cut(s) 117
Sfr274I CTCGAG 1 cut(s) 338
SinI GGWCC 1 cut(s) 725
SlaI CTCGAG 1 cut(s) 338
SmiMI CAYNNNNRTG 1 cut(s) 586
SmlI CTYRAG 1 cut(s) 338
SmoI CTYRAG 1 cut(s) 338
Sse9I AATT 5 cut(s) 345, 501, 627, 657, 775
SseBI AGGCCT 1 cut(s) 120
SsiI CCGC 3 cut(s) 692, 820, 860
SspMI CTAG 1 cut(s) 833
StuI AGGCCT 1 cut(s) 120
StyD4I CCNGG 4 cut(s) 402, 514, 883, 904
TaaI ACNGT 3 cut(s) 541, 826, 869
TaiI ACGT 1 cut(s) 333
TaqI TCGA 3 cut(s) 173, 339, 789
TasI AATT 5 cut(s) 345, 501, 627, 657, 775
TatI WGTACW 1 cut(s) 635
TauI GCSGC 2 cut(s) 695, 822
Tru1I TTAA 2 cut(s) 33, 840
Tru9I TTAA 2 cut(s) 33, 840
TscAI CASTG 1 cut(s) 591
TseFI GTSAC 1 cut(s) 448
TseI GCWGC 1 cut(s) 146
Tsp45I GTSAC 1 cut(s) 448
TspDTI ATGAA 2 cut(s) 463, 681
TspGWI ACGGA 1 cut(s) 309
TspRI CASTG 1 cut(s) 591
VneI GTGCAC 1 cut(s) 567
VpaK11BI GGWCC 1 cut(s) 725
XagI CCTNNNNNAGG 1 cut(s) 243
XceI RCATGY 1 cut(s) 636
XhoI CTCGAG 1 cut(s) 338
XspI CTAG 1 cut(s) 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.