MD08G1217600.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009040
Physical Location & Seq
Reverse (-)
28035994 .. 28041316
5323 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1217600.v1.1.491

Sequence Viewer

Length: 2973 bp
ATGGCATTGTACTTGCTGCTGCCGACTGTGTTCCTCCTCTGTTTGCTTTTCCCGCCATGCATGCCTCTAACGGTCGGAACTGAAACGGAAGCTGAAGCCCTCCTTGAGTTGAAGAGGCAGCTCAAAGACCCCCTGAATGTTCTCGATTCCTGGAAAGAGACCGCAGATCGGTCGCCCTGCAACTTCTTCGGAGTCGCCTGCGATCGGATTAGCGGGAAAGTAATTGGAGTTGCTCTTGATAACAAGTCCCTCTCGGGTGAGATTTCGCCCGCCATTGGTGTTCTTGACAGTCTCGCCACACTTTCGCTGCCTTGCAACAACATTACAGGAAAGCTTCCTGCCCAAGTCACTCGTTGTGCCAATCTCAAAACTTTAAATCTCACCGGCAACAAAATGGTGGGAGCAATCCCGGATCTTTCTGCCCTCGGAAAATTGGAGATTCTTGATCTTTCTACAAACTCCTTCTCCGCGGCATTCCCATCATGGGTGGGGAATCTGACTGCGTTGAATTCCCTCGGGCTCGGGGAAAATGAATTTGATGAAGGTGAAATCCCTGAGGGGCTTGGAAACTTGACCAACTTGACTTGGCTCCATCTGGGGAATTCCCAGTTCAGAGGAGAGATTCCGGAATCTGTTTACAAAATGAAGGCACTGGAGACGTTGGATATGTCGAAGAACAAGCTCTCTGGGAAGCTCTCCAACTCGATTTCCCAGCTGCAAAAACTCAATAAGATTGAGCTTTTTCAAAACAATTTGACTGGGGAAATCCCACCAGGGCTCGCTAATCTCACCCTCCTAAGGGAATTTGATTTCTCATCAAACAAGTTTTATGGGAAGTTGCCTTCCGAGATGGGAAATCTCAAGAACTTGGTGGTTTTTCAGTTATTCAGGAACAATTTCTCCGGGGAATTCCCTGCTGGGTTCGGCGATATGAAACACCTTGTTGCAATCTCAATTTATGAAAACAGATTTTCCGGGGATTTTCCAACCAATTTTGGCAGATTCTCACCTTTGGAAAGCATTGACATATCCGAGAATCAGTTTTCAGGTGGTTTCCCAAGGTTTCTTTGTGAAAAGGGCAGGTTACAATTCTTGCTTGCTTTGGGCAACAATTTTTCTGGGGAGCTGCCAGACTCTTACGTTCGATGTAAATCTCTAGAGAGGCTGAGAGTCAATCAGAACCGTTTGTCTGGGAAGATTCCAGCTGAGTTTTGGTCACTTCCCTACGCAACAATGATTGATTTCAGTGATAACGACTTCAGCGGAGAGATATCCCCCAGCATTGGGTTTTCTACCAGCTTGAATCAGTTGATATTGAAGAACAATGGTTTCTCTGGTTATCTTCCGCAACTTGGGAACCTATCAAACTTGGAGAGGCTCTATCTGAGTGGTAATAAATTTTCCGGTGAAATACCTTCCGAAATTGGTGCTCTAAAGCAGCTGTCATCTTTGCATCTTGAACACAATTCTCTGACAGGATCGGTACCATCAGAATTGGGATACTGTGTTAAGTTGGTGGACGTGAATCTTGCTTCGAATTCTTTAACTGGTAATATCCCACGCACGTTATCACTAATAAGCTCTTTGAACTCTCTAAATCTCTCGCAAAATAGCCTCACGGGTTTGATTCCTGAAACTTTGGTGAAACTGAAGCTAAGTTCTATAGATTTGTCGGGGAATCAGTTATCCGGAAGAGTACCATCTGATCTTCTGACCATTGGAGGAGACAAAGCCTTTGATGGAAACAAGGGACTCTGCATTGATCAATATTCCAGAACACGTACAAATTCTGGGATGAACATGTGTACTGAAAAGCCGAATCAAAAAAAGGTGCTTGAAAATAAACTGGTTTTGTTCTCGATTATAGCATTGGCGTTGGTTGCTGTTTTTGCTGGATTACTGCTGGTTAGTCATAAGAACTTCAAGCTTGGGGAGGCAGTTGACAGAGAAAATAATTTGGAAGCAGGGAAGGAGAAAGATCTGAAATGGAAACTTGCGTCTTTCCACCAGTTGGAAATAGATGCAGATGATATATGCGATTTGAAAGAAGAAAATTTGATTGGAAGTGGCAGTACAGGGAAAGTTTACCGCATAGATTTGAAGAAAGGTCGTGGCACGGTAGCTGTCAAACAACTCTGGAAAGGAGATGGCATGAAGCTTTTGACTGCAGAGATGGACATTTTGGGGAAGATCAGGCATAGAAATATACTGAAGCTATACGCTTGTTCAGCGAATGGCGGATCTAGTGTTCTCGTGTTTGAGTACATGGCAAATGGTAATTTATTTCAAGCGCTTCACAGGGATATTAAAAGCGGGGAGCCCGAATTGGATTGGTGCCAGAGGTATACGATTGCTTTGGGAGCAGCTAGGGGCATTGCTTATCTGCATCATGATTGTTCACCAGCTATAATTCATAGAGATATTAAATCCACCAACATTCTGCTCGACGACGACTATGAGGCAAAAGTTGCTGATTTCGGAGTTGCAAAGATTGCAGAAAGCTCTCAGAAGGGTTGTGATTACAGCTCCCTCGCTGGCACACATGGCTATATTGCTCCTGAGCTGGCTTATACTGCTAAAGTGAACGAAAAGTGCGATGTGTATAGTTTCGGTGTGGTGCTGCTCGAGCTAGTAACTGGCAGGAGACCAGTTGAAGAGGATTACGGTGAAGGAAAGGATATTGTTTACTGGGTTTCAACGCATCTGAGTGACCGTGAAGCGGTTGTTAAGATTCTAGATGATAAAGTGGCACATGAATGTGTCGAGGACGACATGATCAAGGTCTTGAAGGTTGCTGTTCTCTGCACTACCAAGCTACCGTCCTTGCGCCCGACAATGCGGGATGTTATAAAGATGCTTACGGCTGCTGATCCTTGCACTTTCAGGTCCGGAAACAACACCTGTAACGACGAAAACGTCAAGGATTTCTCACCCAGTACTGATGAATTGTCTCTTTTAGCAGTAGCTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

991

Amino Acids

108.34

Weight (kDa)

5.62

Isoelectric Point (pI)

29.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 68 1.2e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 185 - 270 5e-09 Leucine-rich repeat region
LRR_8 PF13855 192 - 252 8.9e-06 Leucine rich repeat
LRR_8 PF13855 505 - 562 1.5e-06 Leucine rich repeat
Pkinase PF00069 683 - 951 1.9e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 683 - 953 8.9e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2849
AasI GACNNNNNNGTC 1 cut(s) 2915
Acc36I ACCTGC 1 cut(s) 1071
Acc65I GGTACC 1 cut(s) 1483
AccB1I GGYRCC 2 cut(s) 1483, 2334
AccB7I CCANNNNNTGG 1 cut(s) 2011
AccI GTMKAC 1 cut(s) 2345
AccII CGCG 1 cut(s) 470
AccIII TCCGGA 3 cut(s) 625, 1688, 2888
AclWI GGATC 4 cut(s) 420, 1486, 2248, 2864
AcsI RAATTY 9 cut(s) 508, 533, 601, 803, 908, 1397, 1537, 1786, 2053
AcuI CTGAAG 4 cut(s) 114, 1243, 1670, 2231
AfaI GTAC 8 cut(s) 11, 1485, 1698, 1783, 1807, 2074, 2264, 2938
AfeI AGCGCT 1 cut(s) 2292
AflIII ACRYGT 2 cut(s) 1778, 1800
AjiI CACGTC 1 cut(s) 1522
AjnI CCWGG 2 cut(s) 149, 772
AjuI GAANNNNNNNTTGG 2 cut(s) 2043, 2075
AloI GAACNNNNNNTCC 4 cut(s) 884, 916, 2232, 2264
Alw21I GWGCWC 1 cut(s) 1432
Alw26I GTCTC 6 cut(s) 152, 296, 650, 1719, 2638, 2955
AlwI GGATC 4 cut(s) 420, 1486, 2248, 2864
Ama87I CYCGRG 4 cut(s) 253, 515, 521, 2624
Aor13HI TCCGGA 3 cut(s) 625, 1688, 2888
Aor51HI AGCGCT 1 cut(s) 2292
ApoI RAATTY 9 cut(s) 508, 533, 601, 803, 908, 1397, 1537, 1786, 2053
ArsI GACNNNNNNTTYG 2 cut(s) 1718, 1750
Asp700I GAANNNNTTC 1 cut(s) 896
Asp718I GGTACC 1 cut(s) 1483
AspLEI GCGC 2 cut(s) 2293, 2829
AspS9I GGNCC 1 cut(s) 2886
AsuC2I CCSGG 3 cut(s) 410, 904, 976
AsuII TTCGAA 1 cut(s) 1535
AsuNHI GCTAGC 1 cut(s) 2966
AvaI CYCGRG 4 cut(s) 253, 515, 521, 2624
AvaII GGWCC 1 cut(s) 2886
AxyI CCTNAGG 2 cut(s) 555, 797
BanI GGYRCC 2 cut(s) 1483, 2334
BanII GRGCYC 3 cut(s) 522, 780, 2322
BarI GAAGNNNNNNTAC 2 cut(s) 2056, 2088
BauI CACGAG 1 cut(s) 2252
Bbv12I GWGCWC 1 cut(s) 1432
BccI CCATC 8 cut(s) 487, 600, 844, 1495, 1708, 1733, 2141, 2167
BceAI ACGGC 1 cut(s) 2877
BcgI CGANNNNNNTGC 6 cut(s) 153, 169, 187, 203, 1409, 1443
BciT130I CCWGG 2 cut(s) 151, 774
BciVI GTATCC 1 cut(s) 1493
BclI TGATCA 2 cut(s) 1762, 2775
BcnI CCSGG 3 cut(s) 410, 904, 976
BcoDI GTCTC 6 cut(s) 152, 296, 650, 1719, 2638, 2955
BfaI CTAG 6 cut(s) 1157, 2244, 2367, 2630, 2735, 2967
BfmI CTRYAG 2 cut(s) 1662, 2166
BfoI RGCGCY 1 cut(s) 2294
BfuAI ACCTGC 1 cut(s) 1071
BfuI GTATCC 1 cut(s) 1493
BglII AGATCT 1 cut(s) 1978
BmcAI AGTACT 1 cut(s) 2938
Bme1390I CCNGG 5 cut(s) 151, 410, 774, 904, 976
Bme18I GGWCC 1 cut(s) 2886
BmeT110I CYCGRG 4 cut(s) 253, 515, 521, 2624
BmgBI CACGTC 1 cut(s) 1522
BmgT120I GGNCC 1 cut(s) 2886
BmiI GGNNCC 5 cut(s) 590, 1358, 1485, 2319, 2336
BmrFI CCNGG 5 cut(s) 151, 410, 774, 904, 976
BmrI ACTGGG 4 cut(s) 601, 768, 2698, 2928
BmsI GCATC 5 cut(s) 1462, 2011, 2395, 2710, 2844
BmtI GCTAGC 1 cut(s) 2970
BmuI ACTGGG 4 cut(s) 601, 768, 2698, 2928
BpmI CTGGAG 1 cut(s) 674
Bpu10I CCTNAGC 1 cut(s) 2559
Bpu14I TTCGAA 1 cut(s) 1535
BpuEI CTTGAG 2 cut(s) 125, 845
BpuMI CCSGG 3 cut(s) 410, 904, 976
BsaAI YACGTR 1 cut(s) 1781
BsaBI GATNNNNATC 1 cut(s) 1150
BsaI GGTCTC 2 cut(s) 152, 2638
BsaJI CCNNGG 7 cut(s) 424, 468, 514, 773, 903, 975, 1058
BsaWI WCCGGW 4 cut(s) 625, 1403, 1688, 2888
BsaXI ACNNNNNCTCC 4 cut(s) 449, 479, 884, 914
Bse118I RCCGGY 1 cut(s) 383
Bse21I CCTNAGG 2 cut(s) 555, 797
Bse3DI GCAATG 1 cut(s) 2373
Bse8I GATNNNNATC 1 cut(s) 1150
BseAI TCCGGA 3 cut(s) 625, 1688, 2888
BseBI CCWGG 2 cut(s) 151, 774
BseDI CCNNGG 7 cut(s) 424, 468, 514, 773, 903, 975, 1058
BseGI GGATG 2 cut(s) 1800, 2848
BseJI GATNNNNATC 1 cut(s) 1150
BseMI GCAATG 1 cut(s) 2373
BseMII CTCAG 7 cut(s) 546, 1157, 1197, 1376, 2519, 2550, 2696
BseRI GAGGAG 3 cut(s) 26, 630, 1737
BseYI CCCAGC 3 cut(s) 711, 917, 1277
BsgI GTGCAG 1 cut(s) 2788
Bsh1236I CGCG 1 cut(s) 470
Bsh1285I CGRYCG 3 cut(s) 75, 173, 205
BshNI GGYRCC 2 cut(s) 1483, 2334
BsiEI CGRYCG 3 cut(s) 75, 173, 205
BsiHKAI GWGCWC 1 cut(s) 1432
BsiHKCI CYCGRG 4 cut(s) 253, 515, 521, 2624
BsiSI CCGG 8 cut(s) 384, 410, 626, 903, 975, 1404, 1689, 2889
BslFI GGGAC 2 cut(s) 232, 1764
BsmAI GTCTC 6 cut(s) 152, 296, 650, 1719, 2638, 2955
BsmBI CGTCTC 1 cut(s) 650
BsmFI GGGAC 2 cut(s) 232, 1764
BsmI GAATGC 1 cut(s) 473
Bso31I GGTCTC 2 cut(s) 152, 2638
BsoBI CYCGRG 4 cut(s) 253, 515, 521, 2624
Bsp119I TTCGAA 1 cut(s) 1535
Bsp1286I GDGCHC 4 cut(s) 522, 780, 1432, 2322
Bsp13I TCCGGA 3 cut(s) 625, 1688, 2888
BspCNI CTCAG 7 cut(s) 547, 1158, 1198, 1377, 2518, 2551, 2697
BspEI TCCGGA 3 cut(s) 625, 1688, 2888
BspFNI CGCG 1 cut(s) 470
BspHI TCATGA 1 cut(s) 2389
BspLI GGNNCC 5 cut(s) 590, 1358, 1485, 2319, 2336
BspMAI CTGCAG 1 cut(s) 2170
BspMI ACCTGC 1 cut(s) 1071
BspOI GCTAGC 1 cut(s) 2970
BspPI GGATC 4 cut(s) 420, 1486, 2248, 2864
BspT104I TTCGAA 1 cut(s) 1535
BspT107I GGYRCC 2 cut(s) 1483, 2334
BspTNI GGTCTC 2 cut(s) 152, 2638
BsrDI GCAATG 1 cut(s) 2373
BsrFI RCCGGY 1 cut(s) 383
BssAI RCCGGY 1 cut(s) 383
BssECI CCNNGG 7 cut(s) 424, 468, 514, 773, 903, 975, 1058
BssNAI GTATAC 1 cut(s) 2346
BssSI CACGAG 1 cut(s) 2252
BssT1I CCWWGG 1 cut(s) 1058
Bst1107I GTATAC 1 cut(s) 2346
Bst2BI CACGAG 1 cut(s) 2252
Bst2UI CCWGG 2 cut(s) 151, 774
Bst4CI ACNGT 9 cut(s) 28, 73, 290, 1184, 1505, 2119, 2666, 2714, 2820
Bst6I CTCTTC 3 cut(s) 107, 1687, 2649
BstAPI GCANNNNNTGC 2 cut(s) 2468, 2492
BstBAI YACGTR 1 cut(s) 1781
BstBI TTCGAA 1 cut(s) 1535
BstC8I GCNNGC 8 cut(s) 62, 199, 270, 780, 1098, 2536, 2565, 2968
BstDEI CTNAG 9 cut(s) 555, 797, 1166, 1206, 1385, 1655, 2505, 2559, 2705
BstDSI CCRYGG 1 cut(s) 468
BstF5I GGATG 2 cut(s) 1800, 2848
BstFNI CGCG 1 cut(s) 470
BstH2I RGCGCY 1 cut(s) 2294
BstHHI GCGC 2 cut(s) 2293, 2829
BstMAI GTCTC 6 cut(s) 152, 296, 650, 1719, 2638, 2955
BstMCI CGRYCG 3 cut(s) 75, 173, 205
BstNI CCWGG 2 cut(s) 151, 774
BstNSI RCATGY 2 cut(s) 64, 1804
BstSCI CCNGG 5 cut(s) 149, 408, 772, 902, 974
BstSFI CTRYAG 2 cut(s) 1662, 2166
BstUI CGCG 1 cut(s) 470
BstX2I RGATCY 3 cut(s) 412, 1978, 2240
BstYI RGATCY 3 cut(s) 412, 1978, 2240
BstZ17I GTATAC 1 cut(s) 2346
Bsu36I CCTNAGG 2 cut(s) 555, 797
BsuI GTATCC 1 cut(s) 1493
BtgI CCRYGG 1 cut(s) 468
BtgZI GCGATG 1 cut(s) 2610
BtrI CACGTC 1 cut(s) 1522
BtsCI GGATG 2 cut(s) 1800, 2848
BtsIMutI CAGTG 2 cut(s) 650, 1252
BveI ACCTGC 1 cut(s) 1071
Cac8I GCNNGC 8 cut(s) 62, 199, 270, 780, 1098, 2536, 2565, 2968
CciI TCATGA 1 cut(s) 2389
CfoI GCGC 2 cut(s) 2293, 2829
Cfr10I RCCGGY 1 cut(s) 383
Cfr13I GGNCC 1 cut(s) 2886
Cfr42I CCGCGG 1 cut(s) 471
CseI GACGC 1 cut(s) 1986
Csp6I GTAC 8 cut(s) 10, 1484, 1697, 1782, 1806, 2073, 2263, 2937
CviQI GTAC 8 cut(s) 10, 1484, 1697, 1782, 1806, 2073, 2263, 2937
DdeI CTNAG 9 cut(s) 555, 797, 1166, 1206, 1385, 1655, 2505, 2559, 2705
DraI TTTAAA 1 cut(s) 375
DrdI GACNNNNNNGTC 1 cut(s) 2915
DseDI GACNNNNNNGTC 1 cut(s) 2915
Eam1104I CTCTTC 3 cut(s) 107, 1687, 2649
EarI CTCTTC 3 cut(s) 107, 1687, 2649
EciI GGCGGA 1 cut(s) 2253
Eco130I CCWWGG 1 cut(s) 1058
Eco24I GRGCYC 3 cut(s) 522, 780, 2322
Eco31I GGTCTC 2 cut(s) 152, 2638
Eco32I GATATC 1 cut(s) 1272
Eco47I GGWCC 1 cut(s) 2886
Eco47III AGCGCT 1 cut(s) 2292
Eco57I CTGAAG 4 cut(s) 114, 1243, 1670, 2231
Eco81I CCTNAGG 2 cut(s) 555, 797
Eco88I CYCGRG 4 cut(s) 253, 515, 521, 2624
EcoRI GAATTC 4 cut(s) 508, 601, 908, 1537
EcoRII CCWGG 2 cut(s) 149, 772
EcoRV GATATC 1 cut(s) 1272
EcoT14I CCWWGG 1 cut(s) 1058
EcoT22I ATGCAT 1 cut(s) 62
EcoT38I GRGCYC 3 cut(s) 522, 780, 2322
ErhI CCWWGG 1 cut(s) 1058
Esp3I CGTCTC 1 cut(s) 650
FalI AAGNNNNNCTT 2 cut(s) 87, 119
FaqI GGGAC 2 cut(s) 232, 1764
FauI CCCGC 5 cut(s) 60, 206, 277, 2306, 2832
FbaI TGATCA 2 cut(s) 1762, 2775
FblI GTMKAC 1 cut(s) 2345
FokI GGATG 2 cut(s) 1807, 2855
FriOI GRGCYC 3 cut(s) 522, 780, 2322
FspBI CTAG 6 cut(s) 1157, 2244, 2367, 2630, 2735, 2967
GlaI GCGC 2 cut(s) 2292, 2828
GsaI CCCAGC 3 cut(s) 715, 921, 1281
GsuI CTGGAG 1 cut(s) 674
HaeII RGCGCY 1 cut(s) 2294
HapII CCGG 8 cut(s) 384, 410, 626, 903, 975, 1404, 1689, 2889
HgaI GACGC 1 cut(s) 1986
HhaI GCGC 2 cut(s) 2293, 2829
Hin6I GCGC 2 cut(s) 2291, 2827
HinP1I GCGC 2 cut(s) 2291, 2827
HincII GTYRAC 1 cut(s) 1942
HindII GTYRAC 1 cut(s) 1942
HindIII AAGCTT 3 cut(s) 332, 1925, 2156
HpaII CCGG 8 cut(s) 384, 410, 626, 903, 975, 1404, 1689, 2889
Hpy166II GTNNAC 9 cut(s) 637, 1519, 1806, 1942, 2086, 2346, 2399, 2584, 2686
Hpy8I GTNNAC 9 cut(s) 637, 1519, 1806, 1942, 2086, 2346, 2399, 2584, 2686
Hpy99I CGWCG 3 cut(s) 2450, 2453, 2912
HpyAV CCTTC 9 cut(s) 472, 536, 640, 852, 1425, 1963, 2503, 2663, 2782
HpyCH4III ACNGT 9 cut(s) 28, 73, 290, 1184, 1505, 2119, 2666, 2714, 2820
HpyCH4IV ACGT 6 cut(s) 659, 1140, 1521, 1565, 1780, 2916
HpyF3I CTNAG 9 cut(s) 555, 797, 1166, 1206, 1385, 1655, 2505, 2559, 2705
HpySE526I ACGT 6 cut(s) 659, 1140, 1521, 1565, 1780, 2916
HspAI GCGC 2 cut(s) 2291, 2827
Kpn2I TCCGGA 3 cut(s) 625, 1688, 2888
KpnI GGTACC 1 cut(s) 1487
Ksp22I TGATCA 2 cut(s) 1762, 2775
KspI CCGCGG 1 cut(s) 471
LmnI GCTCC 7 cut(s) 401, 594, 1123, 2317, 2360, 2531, 2560
LweI GCATC 5 cut(s) 1462, 2011, 2395, 2710, 2844
MaeI CTAG 6 cut(s) 1157, 2244, 2367, 2630, 2735, 2967
MaeII ACGT 6 cut(s) 659, 1140, 1521, 1565, 1780, 2916
MaeIII GTNAC 6 cut(s) 346, 1083, 1215, 2632, 2708, 2903
MflI RGATCY 3 cut(s) 412, 1978, 2240
MhlI GDGCHC 4 cut(s) 522, 780, 1432, 2322
MlyI GAGTC 4 cut(s) 201, 1127, 1179, 1746
MmeI TCCRAC 5 cut(s) 55, 642, 723, 1010, 1992
Mph1103I ATGCAT 1 cut(s) 62
MroI TCCGGA 3 cut(s) 625, 1688, 2888
MroXI GAANNNNTTC 1 cut(s) 896
MseI TTAA 6 cut(s) 374, 1509, 1544, 2307, 2424, 2727
MslI CAYNNNNRTG 3 cut(s) 2706, 2755, 2757
MspA1I CMGCKG 5 cut(s) 470, 715, 1205, 1263, 1441
MspI CCGG 8 cut(s) 384, 410, 626, 903, 975, 1404, 1689, 2889
MspR9I CCNGG 5 cut(s) 151, 410, 774, 904, 976
Mva1269I GAATGC 1 cut(s) 473
MvaI CCWGG 2 cut(s) 151, 774
MvnI CGCG 1 cut(s) 470
NciI CCSGG 3 cut(s) 410, 904, 976
NheI GCTAGC 1 cut(s) 2966
NlaIV GGNNCC 5 cut(s) 590, 1358, 1485, 2319, 2336
NmuCI GTSAC 3 cut(s) 346, 1215, 2708
NsiI ATGCAT 1 cut(s) 62
NspI RCATGY 2 cut(s) 64, 1804
NspV TTCGAA 1 cut(s) 1535
PaeI GCATGC 1 cut(s) 64
PaeR7I CTCGAG 1 cut(s) 2624
PagI TCATGA 1 cut(s) 2389
PciI ACATGT 1 cut(s) 1800
PcsI WCGNNNNNNNCGW 2 cut(s) 2592, 2913
PctI GAATGC 1 cut(s) 473
PdmI GAANNNNTTC 1 cut(s) 896
PflMI CCANNNNNTGG 1 cut(s) 2011
PfoI TCCNGGA 2 cut(s) 149, 408
Ple19I CGATCG 1 cut(s) 205
PleI GAGTC 4 cut(s) 200, 1127, 1178, 1746
PpsI GAGTC 4 cut(s) 200, 1127, 1178, 1746
Ppu21I YACGTR 1 cut(s) 1781
PscI ACATGT 1 cut(s) 1800
PsiI TTATAA 1 cut(s) 2849
Psp6I CCWGG 2 cut(s) 149, 772
PspFI CCCAGC 3 cut(s) 711, 917, 1277
PspGI CCWGG 2 cut(s) 149, 772
PspN4I GGNNCC 5 cut(s) 590, 1358, 1485, 2319, 2336
PspPI GGNCC 1 cut(s) 2886
PspXI VCTCGAGB 1 cut(s) 2624
PstI CTGCAG 1 cut(s) 2170
PsuI RGATCY 3 cut(s) 412, 1978, 2240
PvuI CGATCG 1 cut(s) 205
PvuII CAGCTG 3 cut(s) 715, 1205, 1441
RsaI GTAC 8 cut(s) 11, 1485, 1698, 1783, 1807, 2074, 2264, 2938
RsaNI GTAC 8 cut(s) 10, 1484, 1697, 1782, 1806, 2073, 2263, 2937
RseI CAYNNNNRTG 3 cut(s) 2706, 2755, 2757
SacII CCGCGG 1 cut(s) 471
SaqAI TTAA 6 cut(s) 374, 1509, 1544, 2307, 2424, 2727
Sau96I GGNCC 1 cut(s) 2886
ScaI AGTACT 1 cut(s) 2938
SchI GAGTC 4 cut(s) 201, 1127, 1179, 1746
ScrFI CCNGG 5 cut(s) 151, 410, 774, 904, 976
SduI GDGCHC 4 cut(s) 522, 780, 1432, 2322
SfaNI GCATC 5 cut(s) 1462, 2011, 2395, 2710, 2844
SfcI CTRYAG 2 cut(s) 1662, 2166
Sfr274I CTCGAG 1 cut(s) 2624
Sfr303I CCGCGG 1 cut(s) 471
SfuI TTCGAA 1 cut(s) 1535
SgrBI CCGCGG 1 cut(s) 471
SinI GGWCC 1 cut(s) 2886
SlaI CTCGAG 1 cut(s) 2624
SmiMI CAYNNNNRTG 3 cut(s) 2706, 2755, 2757
SmlI CTYRAG 3 cut(s) 104, 860, 2624
SmoI CTYRAG 3 cut(s) 104, 860, 2624
SphI GCATGC 1 cut(s) 64
SspI AATATT 1 cut(s) 1769
SspMI CTAG 6 cut(s) 1157, 2244, 2367, 2630, 2735, 2967
StyD4I CCNGG 5 cut(s) 149, 408, 772, 902, 974
StyI CCWWGG 1 cut(s) 1058
TaaI ACNGT 9 cut(s) 28, 73, 290, 1184, 1505, 2119, 2666, 2714, 2820
TaiI ACGT 6 cut(s) 662, 1143, 1524, 1568, 1783, 2919
TaqI TCGA 9 cut(s) 144, 671, 704, 1144, 1535, 1859, 2445, 2625, 2763
TaqII GACCGA 1 cut(s) 159
TatI WGTACW 5 cut(s) 9, 1805, 2072, 2262, 2936
TauI GCSGC 1 cut(s) 473
Tru1I TTAA 6 cut(s) 374, 1509, 1544, 2307, 2424, 2727
Tru9I TTAA 6 cut(s) 374, 1509, 1544, 2307, 2424, 2727
TscAI CASTG 2 cut(s) 657, 1252
TseFI GTSAC 3 cut(s) 346, 1215, 2708
Tsp45I GTSAC 3 cut(s) 346, 1215, 2708
TspGWI ACGGA 1 cut(s) 101
TspRI CASTG 2 cut(s) 657, 1252
Van91I CCANNNNNTGG 1 cut(s) 2011
VpaK11BI GGWCC 1 cut(s) 2886
XapI RAATTY 9 cut(s) 508, 533, 601, 803, 908, 1397, 1537, 1786, 2053
XbaI TCTAGA 2 cut(s) 1156, 2734
XceI RCATGY 2 cut(s) 64, 1804
XcmI CCANNNNNNNNNTGG 1 cut(s) 1209
XhoI CTCGAG 1 cut(s) 2624
XmiI GTMKAC 1 cut(s) 2345
XmnI GAANNNNTTC 1 cut(s) 896
XspI CTAG 6 cut(s) 1157, 2244, 2367, 2630, 2735, 2967
ZrmI AGTACT 1 cut(s) 2938
Zsp2I ATGCAT 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.