pycom08g18860
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0009040
Physical Location & Seq
Reverse (-)
18925535 .. 18928823
3289 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g18860.1

Sequence Viewer

Length: 2973 bp
ATGGCATTGTACTTGCTGCTGCCGACTGTGGTCTTCCTCTGTTTGCTTTTCCCGCCATGCATGCCTCTAACGGTCGGAACTGAAACGGAAGCTGAAGCCCTCCTTGAGTTGAAGAGGCAACTCAAAGACCCTCTGAATGTTCTCGATTCCTGGAAAGAGACCGCAGATGGGTCGCCCTGCAACTTCTTCGGAGTCGCCTGTGATCGGATTAGCGGGAAAGTGATTGGAATTTCTCTTGATAACAAGTCCCTCTCGGGTGAGATTTCGCCCGCCATTGGTGTTCTTGACAGCCTCGCCACACTTTCGCTGCCTTGCAACAACATTACAGGAAAGCTTCCTGCCCAAGTCACTGGTTGTGCCAATCTCAAAACTTTGAATCTCACTGGCAACAAAATGGTGGGAGCAATCCCGGATCTTTCTGCCCTCCGAAAATTGGAGATTCTTGATCTTTCTACAAACTCCTTCTCCGCCGCATTCCCATCATGGGTGGGGAATCTGTCTGCGTTGAATTCTCTCGGGCTTGGGGAAAATGAATTTGATGAAGGTGAAATCCCTGAGGGGCTTGGAAACTTGACCAACTTGACTTGGCTCCATCTGGGGAATTCCCAGTTCAGAGGAGAGATTCCGGAATCTGTTTACAAAATGAAGGCATTGGAGACGTTGGATATGTCGAAGAACAAGCTCTCTGGGAAGCTCTCCAACTCGATTTCCCAGCTACAAAAACTCAATAAGATTGAGCTTTTTCGAAACAATTTGACCGGGGAAATCCCACCAGGGCTCGCTAATCTCACCCTCCTAAGGGAATTTGATTTCTCATCAAACAAGTTTTATGGGAAGTTGCCTTCGGAGATGGGAAATCTCAAGAACTTGGTGGTTTTTCAGTTATTTGGGAACAATTTCTCCGGGGAATTCCCTGCTGGGTTCGGCGATATGGAACACCTTGTTGCAATCTCAATTTATGAAAACAGATTTTCCGGGGAATTTCCAACCAATTTTGGCAGATTCTCACCTTTGGAAAGCATTGACATATCCGAGAATCAGTTTTCGGGTGGTTTCCCAAGGTTTCTTTGTGAAAAGGGCAGGTTACAATTCTTACTTGCTTTGGGCAACAATTTTTCTGGGGAGCTGCCAGACTCTTACGTTCGATGTAAATCTCTAGAGAGGCTGAGAGTCAATCAGAACAATTTGTCTGGGAAGATTCCAGCTGAGTTTTGGTCACTTCCCTACGCGACAATGATTGACTTGAGTGATAACGACTTCAGCGGAGAGATCTCCCCCAGCATTGGGTTTTCTACCAGCTTGAATCAGTTGAAATTGCAGAACAATGGTTTCTCTGGTAATCTTCCGCAACTTGGGAACCTATCAAACTTGGAGAGGCTCTATCTGAGTGGTAATAAATTTTCCGGTGAAATACCTTCCGAAATTGGTGCTCTAAAGCAGCTGTCATCTTTGCATCTTGAACACAATTCTTTGACAGGATCGGTACCATCAGAATTGGGATACTGTGATAAGTTGGTGGACGTGAATCTTGCTTCGAATTCTTTAACTGGTAATATCCCACGTACGTTATCACAAATAAGCTCTTTGAACTCTCTAAATCTCTCGCAAAATAACCTCACGGGTTTGATTCCCGAAACTTTGGTGAAACTGAAGCTAAGTTCTATAGATTTGTCGGGGAATCAGTTATCCGGAAGGGTACCATCTGATCTTCTGACCATGGGAGGAGACAAAGCCTTTGATGGAAACAAGGGACTCTGCATTGATCAATATTCCAGAACACGTACAAATTCTGGGATGAACATGTGTACTGAAAAGCCGAATCAAAAAAGGGTGCTTGAAAATAAACTGGTTTTGTTCTCGATTATAGCATTGGCGTTGGTTGCTGTTTTTGCTGGATTACTGCTGGTCAGTTATAAGAACTTCAAGCTTGGGGAGGCAGTTGACAGAGAAAATAATTTGGATGCAGGGAAGGAGAAAGATCTGAAATGGAAACTTGCGTCTTTCCACCAGTTGGAAATAGATGCAGATGACATATGCGATTTGAAAGAAGAAAATTTGATTGGAAGTGGCAGTACAGGGAAAGTTTACCGCATAGATTTGAAGAAAGGTCGTGGCACGGTAGCTGTCAAACAACTCTGGAAAGGAGATGGCATGAAGCTTTTGACCGCAGAGATGGACATTTTGGGGAAGATCAGGCATAGAAATATACTGAAGCTATACGCTTGTTCAGTGATGGATGGATCTAGTGTTCTCGTGTTTGAGTACATGGCAAATGGTAATTTATTTCAAGCGCTTCACAGAGATATTAAAACCGGGGGGCCCGAATTGGATTGGTGCCAGAGGTATAAGATTGTTTTGGGAGCAGCTAGGGGCATTGCTTATCTGCATCATGATTGTTCACCAGCTATAATTCATAGAGATATTAAATCCACTAACATTCTGCTCGACGACGATTATGAGGCAAAAGTTGCTGATTTCGGGGTTGCAAAGATTGCAGAAAGCTCTCAGAAGGGTTGTGATTACAGCTCCTTCGCCGGCACACATGGCTATATTGCTCCTGAGCTGGCATATACTGCTAAAGTGAACGAAAAGTGCGATGTGTATAGTTTCGGTGTGGTGCTGCTCGAGCTAGTAACTGGCAGGAGACCAGTTGAAGAGGATTACGGTGAAGGCAAGGATATTGTTTACTGGGTTTCAAAGCATCTGAGTGACCGTGAAGCGGTTGTTAAGATTCTAGATGATAAAGTGGCACATGAATGTGTCGAGGACGACATGATCAAGGTCTTGAAGGTTGCTGTTCTCTGTACTACCAAGCTACCGTCCTTGCGCCCGACAATGCGGGATGTTATAAAGATGCTTACGGCTGCTGATCCTTGCACTTTCAGGTCCGGAAACAACACCTGTAACGACGAAAATGTCAAGGATTTCTCACCCAGTACTGATGAATTGTCTCTTTTAGCAGTAGCTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

991

Amino Acids

108.36

Weight (kDa)

5.43

Isoelectric Point (pI)

29.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 68 9.8e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 143 - 203 8.4e-06 Leucine rich repeat
LRR_14 PF23598 185 - 270 6.2e-09 Leucine-rich repeat region
LRR_8 PF13855 192 - 252 8.3e-06 Leucine rich repeat
LRR_14 PF23598 444 - 537 2.2e-06 Leucine-rich repeat region
LRR_8 PF13855 504 - 562 1.1e-07 Leucine rich repeat
Pkinase PF00069 683 - 951 1.1e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 683 - 953 2.3e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1914, 2849
AasI GACNNNNNNGTC 1 cut(s) 2915
Acc36I ACCTGC 1 cut(s) 1071
Acc65I GGTACC 2 cut(s) 1483, 1696
AccB1I GGYRCC 3 cut(s) 1483, 1696, 2334
AccB7I CCANNNNNTGG 1 cut(s) 2011
AccII CGCG 1 cut(s) 1229
AccIII TCCGGA 3 cut(s) 625, 1688, 2888
AclWI GGATC 4 cut(s) 420, 1486, 2248, 2864
AcuI CTGAAG 4 cut(s) 114, 1243, 1670, 2231
AfeI AGCGCT 1 cut(s) 2292
AflIII ACRYGT 2 cut(s) 1778, 1800
AjiI CACGTC 1 cut(s) 1522
AjnI CCWGG 2 cut(s) 149, 772
AjuI GAANNNNNNNTTGG 2 cut(s) 2043, 2075
AloI GAACNNNNNNTCC 4 cut(s) 884, 916, 2232, 2264
Alw21I GWGCWC 1 cut(s) 1432
Alw26I GTCTC 5 cut(s) 152, 650, 1719, 2638, 2955
AlwI GGATC 4 cut(s) 420, 1486, 2248, 2864
Ama87I CYCGRG 3 cut(s) 253, 515, 2624
Aor13HI TCCGGA 3 cut(s) 625, 1688, 2888
Aor51HI AGCGCT 1 cut(s) 2292
AoxI GGCC 1 cut(s) 2318
ApaI GGGCCC 1 cut(s) 2322
ApeKI GCWGC 8 cut(s) 16, 19, 307, 1126, 1438, 2363, 2620, 2864
ArsI GACNNNNNNTTYG 2 cut(s) 1718, 1750
Asp700I GAANNNNTTC 1 cut(s) 896
Asp718I GGTACC 2 cut(s) 1483, 1696
AspLEI GCGC 2 cut(s) 2293, 2829
AspS9I GGNCC 3 cut(s) 2318, 2319, 2886
AsuC2I CCSGG 5 cut(s) 410, 760, 904, 976, 2314
AsuHPI GGTGA 9 cut(s) 269, 557, 781, 999, 1418, 1654, 2391, 2678, 2922
AsuII TTCGAA 2 cut(s) 745, 1535
AsuNHI GCTAGC 1 cut(s) 2966
AvaI CYCGRG 3 cut(s) 253, 515, 2624
AvaII GGWCC 1 cut(s) 2886
AxyI CCTNAGG 2 cut(s) 555, 797
BaeGI GKGCMC 1 cut(s) 2322
BanI GGYRCC 3 cut(s) 1483, 1696, 2334
BanII GRGCYC 2 cut(s) 780, 2322
BarI GAAGNNNNNNTAC 2 cut(s) 2056, 2088
BauI CACGAG 1 cut(s) 2252
BbsI GAAGAC 1 cut(s) 25
Bbv12I GWGCWC 1 cut(s) 1432
BbvI GCAGC 8 cut(s) 3, 6, 294, 1113, 1450, 2375, 2607, 2851
BceAI ACGGC 1 cut(s) 2877
BcgI CGANNNNNNTGC 6 cut(s) 153, 169, 187, 203, 1409, 1443
BciT130I CCWGG 2 cut(s) 151, 774
BciVI GTATCC 1 cut(s) 1493
BclI TGATCA 2 cut(s) 1762, 2775
BcnI CCSGG 5 cut(s) 410, 760, 904, 976, 2314
BcoDI GTCTC 5 cut(s) 152, 650, 1719, 2638, 2955
BfaI CTAG 6 cut(s) 1157, 2244, 2367, 2630, 2735, 2967
BfmI CTRYAG 1 cut(s) 1662
BfoI RGCGCY 1 cut(s) 2294
BfuAI ACCTGC 1 cut(s) 1071
BfuI GTATCC 1 cut(s) 1493
BglII AGATCT 2 cut(s) 1269, 1978
BisI GCNGC 9 cut(s) 17, 20, 308, 471, 1127, 1439, 2364, 2621, 2865
BlsI GCNGC 9 cut(s) 18, 21, 309, 472, 1128, 1440, 2365, 2622, 2866
BmcAI AGTACT 1 cut(s) 2938
Bme1390I CCNGG 7 cut(s) 151, 410, 760, 774, 904, 976, 2314
Bme18I GGWCC 1 cut(s) 2886
BmeT110I CYCGRG 3 cut(s) 253, 515, 2624
BmgBI CACGTC 1 cut(s) 1522
BmgT120I GGNCC 3 cut(s) 2318, 2319, 2886
BmiI GGNNCC 7 cut(s) 590, 1358, 1485, 1698, 2319, 2320, 2336
BmrFI CCNGG 7 cut(s) 151, 410, 760, 774, 904, 976, 2314
BmrI ACTGGG 3 cut(s) 601, 2698, 2928
BmsI GCATC 6 cut(s) 1462, 1951, 2011, 2395, 2710, 2844
BmtI GCTAGC 1 cut(s) 2970
BmuI ACTGGG 3 cut(s) 601, 2698, 2928
BoxI GACNNNNGTC 1 cut(s) 29
BpiI GAAGAC 1 cut(s) 25
Bpu10I CCTNAGC 1 cut(s) 2559
Bpu14I TTCGAA 2 cut(s) 745, 1535
BpuEI CTTGAG 3 cut(s) 125, 845, 1264
BpuMI CCSGG 5 cut(s) 410, 760, 904, 976, 2314
BsaAI YACGTR 2 cut(s) 1562, 1781
BsaBI GATNNNNATC 1 cut(s) 1150
BsaI GGTCTC 2 cut(s) 152, 2638
BsaJI CCNNGG 7 cut(s) 759, 773, 903, 975, 1058, 1716, 2313
BsaWI WCCGGW 4 cut(s) 625, 1403, 1688, 2888
BsaXI ACNNNNNCTCC 4 cut(s) 449, 479, 884, 914
Bse118I RCCGGY 1 cut(s) 2534
Bse21I CCTNAGG 2 cut(s) 555, 797
Bse3DI GCAATG 1 cut(s) 2373
Bse8I GATNNNNATC 1 cut(s) 1150
BseAI TCCGGA 3 cut(s) 625, 1688, 2888
BseBI CCWGG 2 cut(s) 151, 774
BseDI CCNNGG 7 cut(s) 759, 773, 903, 975, 1058, 1716, 2313
BseGI GGATG 4 cut(s) 1800, 1966, 2242, 2848
BseJI GATNNNNATC 1 cut(s) 1150
BseMI GCAATG 1 cut(s) 2373
BseMII CTCAG 7 cut(s) 546, 1157, 1197, 1376, 2519, 2550, 2696
BseRI GAGGAG 2 cut(s) 630, 1737
BseSI GKGCMC 1 cut(s) 2322
BseXI GCAGC 8 cut(s) 3, 6, 294, 1113, 1450, 2375, 2607, 2851
BseYI CCCAGC 3 cut(s) 711, 917, 1277
Bsh1236I CGCG 1 cut(s) 1229
Bsh1285I CGRYCG 1 cut(s) 75
BshFI GGCC 1 cut(s) 2320
BshNI GGYRCC 3 cut(s) 1483, 1696, 2334
BsiEI CGRYCG 1 cut(s) 75
BsiHKAI GWGCWC 1 cut(s) 1432
BsiHKCI CYCGRG 3 cut(s) 253, 515, 2624
BsiWI CGTACG 1 cut(s) 1562
BslFI GGGAC 2 cut(s) 232, 1764
BsmAI GTCTC 5 cut(s) 152, 650, 1719, 2638, 2955
BsmBI CGTCTC 1 cut(s) 650
BsmFI GGGAC 2 cut(s) 232, 1764
BsmI GAATGC 1 cut(s) 473
BsnI GGCC 1 cut(s) 2320
Bso31I GGTCTC 2 cut(s) 152, 2638
BsoBI CYCGRG 3 cut(s) 253, 515, 2624
Bsp119I TTCGAA 2 cut(s) 745, 1535
Bsp120I GGGCCC 1 cut(s) 2318
Bsp1286I GDGCHC 3 cut(s) 780, 1432, 2322
Bsp13I TCCGGA 3 cut(s) 625, 1688, 2888
Bsp19I CCATGG 1 cut(s) 1716
BspANI GGCC 1 cut(s) 2320
BspCNI CTCAG 7 cut(s) 547, 1158, 1198, 1377, 2518, 2551, 2697
BspEI TCCGGA 3 cut(s) 625, 1688, 2888
BspFNI CGCG 1 cut(s) 1229
BspHI TCATGA 1 cut(s) 2389
BspLI GGNNCC 7 cut(s) 590, 1358, 1485, 1698, 2319, 2320, 2336
BspMI ACCTGC 1 cut(s) 1071
BspOI GCTAGC 1 cut(s) 2970
BspPI GGATC 4 cut(s) 420, 1486, 2248, 2864
BspT104I TTCGAA 2 cut(s) 745, 1535
BspT107I GGYRCC 3 cut(s) 1483, 1696, 2334
BspTNI GGTCTC 2 cut(s) 152, 2638
BsrDI GCAATG 1 cut(s) 2373
BsrFI RCCGGY 1 cut(s) 2534
BssAI RCCGGY 1 cut(s) 2534
BssECI CCNNGG 7 cut(s) 759, 773, 903, 975, 1058, 1716, 2313
BssSI CACGAG 1 cut(s) 2252
BssT1I CCWWGG 2 cut(s) 1058, 1716
Bst2BI CACGAG 1 cut(s) 2252
Bst2UI CCWGG 2 cut(s) 151, 774
Bst4CI ACNGT 7 cut(s) 28, 73, 1505, 2119, 2666, 2714, 2820
Bst6I CTCTTC 2 cut(s) 107, 2649
BstAPI GCANNNNNTGC 3 cut(s) 2468, 2492, 2573
BstBAI YACGTR 2 cut(s) 1562, 1781
BstBI TTCGAA 2 cut(s) 745, 1535
BstC8I GCNNGC 6 cut(s) 62, 270, 780, 2536, 2565, 2968
BstDEI CTNAG 9 cut(s) 555, 797, 1166, 1206, 1385, 1655, 2505, 2559, 2705
BstDSI CCRYGG 1 cut(s) 1716
BstF5I GGATG 4 cut(s) 1800, 1966, 2242, 2848
BstFNI CGCG 1 cut(s) 1229
BstH2I RGCGCY 1 cut(s) 2294
BstHHI GCGC 2 cut(s) 2293, 2829
BstMAI GTCTC 5 cut(s) 152, 650, 1719, 2638, 2955
BstMCI CGRYCG 1 cut(s) 75
BstMWI GCNNNNNNNGC 9 cut(s) 52, 61, 1880, 1889, 2468, 2492, 2544, 2573, 2626
BstNI CCWGG 2 cut(s) 151, 774
BstNSI RCATGY 2 cut(s) 64, 1804
BstPAI GACNNNNGTC 1 cut(s) 29
BstSCI CCNGG 7 cut(s) 149, 408, 758, 772, 902, 974, 2312
BstSFI CTRYAG 1 cut(s) 1662
BstSLI GKGCMC 1 cut(s) 2322
BstUI CGCG 1 cut(s) 1229
BstV1I GCAGC 8 cut(s) 3, 6, 294, 1113, 1450, 2375, 2607, 2851
BstV2I GAAGAC 1 cut(s) 25
BstX2I RGATCY 4 cut(s) 412, 1269, 1978, 2240
BstXI CCANNNNNNTGG 1 cut(s) 350
BstYI RGATCY 4 cut(s) 412, 1269, 1978, 2240
Bsu36I CCTNAGG 2 cut(s) 555, 797
BsuI GTATCC 1 cut(s) 1493
BsuRI GGCC 1 cut(s) 2320
BtgI CCRYGG 1 cut(s) 1716
BtgZI GCGATG 1 cut(s) 2610
BtrI CACGTC 1 cut(s) 1522
BtsCI GGATG 4 cut(s) 1800, 1966, 2242, 2848
BtsIMutI CAGTG 3 cut(s) 348, 381, 2235
BveI ACCTGC 1 cut(s) 1071
Cac8I GCNNGC 6 cut(s) 62, 270, 780, 2536, 2565, 2968
CciI TCATGA 1 cut(s) 2389
CfoI GCGC 2 cut(s) 2293, 2829
Cfr10I RCCGGY 1 cut(s) 2534
Cfr13I GGNCC 3 cut(s) 2318, 2319, 2886
CseI GACGC 1 cut(s) 1986
DdeI CTNAG 9 cut(s) 555, 797, 1166, 1206, 1385, 1655, 2505, 2559, 2705
DrdI GACNNNNNNGTC 1 cut(s) 2915
DseDI GACNNNNNNGTC 1 cut(s) 2915
Eam1104I CTCTTC 2 cut(s) 107, 2649
EarI CTCTTC 2 cut(s) 107, 2649
EciI GGCGGA 1 cut(s) 457
Eco130I CCWWGG 2 cut(s) 1058, 1716
Eco24I GRGCYC 2 cut(s) 780, 2322
Eco31I GGTCTC 2 cut(s) 152, 2638
Eco47I GGWCC 1 cut(s) 2886
Eco47III AGCGCT 1 cut(s) 2292
Eco57I CTGAAG 4 cut(s) 114, 1243, 1670, 2231
Eco81I CCTNAGG 2 cut(s) 555, 797
Eco88I CYCGRG 3 cut(s) 253, 515, 2624
EcoO109I RGGNCCY 1 cut(s) 2318
EcoRI GAATTC 4 cut(s) 508, 601, 908, 1537
EcoRII CCWGG 2 cut(s) 149, 772
EcoT14I CCWWGG 2 cut(s) 1058, 1716
EcoT22I ATGCAT 1 cut(s) 62
EcoT38I GRGCYC 2 cut(s) 780, 2322
ErhI CCWWGG 2 cut(s) 1058, 1716
Esp3I CGTCTC 1 cut(s) 650
FalI AAGNNNNNCTT 2 cut(s) 87, 119
FaqI GGGAC 2 cut(s) 232, 1764
FauI CCCGC 4 cut(s) 60, 206, 277, 2832
FauNDI CATATG 1 cut(s) 2033
FbaI TGATCA 2 cut(s) 1762, 2775
Fnu4HI GCNGC 9 cut(s) 17, 20, 308, 471, 1127, 1439, 2364, 2621, 2865
FokI GGATG 4 cut(s) 1807, 1973, 2249, 2855
FriOI GRGCYC 2 cut(s) 780, 2322
Fsp4HI GCNGC 9 cut(s) 17, 20, 308, 471, 1127, 1439, 2364, 2621, 2865
FspBI CTAG 6 cut(s) 1157, 2244, 2367, 2630, 2735, 2967
GlaI GCGC 2 cut(s) 2292, 2828
GluI GCNGC 9 cut(s) 17, 20, 308, 471, 1127, 1439, 2364, 2621, 2865
GsaI CCCAGC 3 cut(s) 715, 921, 1281
HaeII RGCGCY 1 cut(s) 2294
HaeIII GGCC 1 cut(s) 2320
HgaI GACGC 1 cut(s) 1986
HhaI GCGC 2 cut(s) 2293, 2829
Hin6I GCGC 2 cut(s) 2291, 2827
HinP1I GCGC 2 cut(s) 2291, 2827
HincII GTYRAC 1 cut(s) 1942
HindII GTYRAC 1 cut(s) 1942
HindIII AAGCTT 3 cut(s) 332, 1925, 2156
HphI GGTGA 9 cut(s) 269, 557, 781, 999, 1418, 1654, 2391, 2678, 2922
Hpy166II GTNNAC 8 cut(s) 637, 1519, 1806, 1942, 2086, 2399, 2584, 2686
Hpy8I GTNNAC 8 cut(s) 637, 1519, 1806, 1942, 2086, 2399, 2584, 2686
Hpy99I CGWCG 3 cut(s) 2450, 2453, 2912
HpyCH4III ACNGT 7 cut(s) 28, 73, 1505, 2119, 2666, 2714, 2820
HpyCH4IV ACGT 6 cut(s) 659, 1140, 1521, 1561, 1565, 1780
HpyF10VI GCNNNNNNNGC 9 cut(s) 52, 61, 1880, 1889, 2468, 2492, 2544, 2573, 2626
HpyF3I CTNAG 9 cut(s) 555, 797, 1166, 1206, 1385, 1655, 2505, 2559, 2705
HpySE526I ACGT 6 cut(s) 659, 1140, 1521, 1561, 1565, 1780
HspAI GCGC 2 cut(s) 2291, 2827
Kpn2I TCCGGA 3 cut(s) 625, 1688, 2888
KpnI GGTACC 2 cut(s) 1487, 1700
KroI GCCGGC 1 cut(s) 2534
KroNI GCCGGC 1 cut(s) 2536
Ksp22I TGATCA 2 cut(s) 1762, 2775
LmnI GCTCC 6 cut(s) 401, 594, 1123, 2360, 2531, 2560
Lsp1109I GCAGC 8 cut(s) 3, 6, 294, 1113, 1450, 2375, 2607, 2851
LweI GCATC 6 cut(s) 1462, 1951, 2011, 2395, 2710, 2844
MaeI CTAG 6 cut(s) 1157, 2244, 2367, 2630, 2735, 2967
MaeII ACGT 6 cut(s) 659, 1140, 1521, 1561, 1565, 1780
MaeIII GTNAC 6 cut(s) 346, 1083, 1215, 2632, 2708, 2903
MflI RGATCY 4 cut(s) 412, 1269, 1978, 2240
MhlI GDGCHC 3 cut(s) 780, 1432, 2322
MlyI GAGTC 4 cut(s) 201, 1127, 1179, 1746
MmeI TCCRAC 5 cut(s) 55, 642, 723, 1010, 1992
Mph1103I ATGCAT 1 cut(s) 62
MroI TCCGGA 3 cut(s) 625, 1688, 2888
MroNI GCCGGC 1 cut(s) 2534
MroXI GAANNNNTTC 1 cut(s) 896
MseI TTAA 4 cut(s) 1544, 2307, 2424, 2727
MslI CAYNNNNRTG 3 cut(s) 2706, 2755, 2757
MspA1I CMGCKG 3 cut(s) 1205, 1263, 1441
MspR9I CCNGG 7 cut(s) 151, 410, 760, 774, 904, 976, 2314
Mva1269I GAATGC 1 cut(s) 473
MvaI CCWGG 2 cut(s) 151, 774
MvnI CGCG 1 cut(s) 1229
MwoI GCNNNNNNNGC 9 cut(s) 52, 61, 1880, 1889, 2468, 2492, 2544, 2573, 2626
NaeI GCCGGC 1 cut(s) 2536
NciI CCSGG 5 cut(s) 410, 760, 904, 976, 2314
NcoI CCATGG 1 cut(s) 1716
NdeI CATATG 1 cut(s) 2033
NgoMIV GCCGGC 1 cut(s) 2534
NheI GCTAGC 1 cut(s) 2966
NlaIV GGNNCC 7 cut(s) 590, 1358, 1485, 1698, 2319, 2320, 2336
NmuCI GTSAC 3 cut(s) 346, 1215, 2708
NsiI ATGCAT 1 cut(s) 62
NspI RCATGY 2 cut(s) 64, 1804
NspV TTCGAA 2 cut(s) 745, 1535
PaeI GCATGC 1 cut(s) 64
PaeR7I CTCGAG 1 cut(s) 2624
PagI TCATGA 1 cut(s) 2389
PciI ACATGT 1 cut(s) 1800
PcsI WCGNNNNNNNCGW 1 cut(s) 2592
PctI GAATGC 1 cut(s) 473
PdiI GCCGGC 1 cut(s) 2536
PdmI GAANNNNTTC 1 cut(s) 896
Pfl23II CGTACG 1 cut(s) 1562
PflMI CCANNNNNTGG 1 cut(s) 2011
PfoI TCCNGGA 2 cut(s) 149, 408
PkrI GCNGC 9 cut(s) 18, 21, 309, 472, 1128, 1440, 2365, 2622, 2866
PleI GAGTC 4 cut(s) 200, 1127, 1178, 1746
PpsI GAGTC 4 cut(s) 200, 1127, 1178, 1746
Ppu21I YACGTR 2 cut(s) 1562, 1781
PscI ACATGT 1 cut(s) 1800
PshAI GACNNNNGTC 1 cut(s) 29
PsiI TTATAA 2 cut(s) 1914, 2849
Psp6I CCWGG 2 cut(s) 149, 772
PspFI CCCAGC 3 cut(s) 711, 917, 1277
PspGI CCWGG 2 cut(s) 149, 772
PspLI CGTACG 1 cut(s) 1562
PspN4I GGNNCC 7 cut(s) 590, 1358, 1485, 1698, 2319, 2320, 2336
PspOMI GGGCCC 1 cut(s) 2318
PspPI GGNCC 3 cut(s) 2318, 2319, 2886
PspXI VCTCGAGB 1 cut(s) 2624
PsuI RGATCY 4 cut(s) 412, 1269, 1978, 2240
PvuII CAGCTG 2 cut(s) 1205, 1441
RseI CAYNNNNRTG 3 cut(s) 2706, 2755, 2757
SaqAI TTAA 4 cut(s) 1544, 2307, 2424, 2727
SatI GCNGC 9 cut(s) 17, 20, 308, 471, 1127, 1439, 2364, 2621, 2865
Sau96I GGNCC 3 cut(s) 2318, 2319, 2886
ScaI AGTACT 1 cut(s) 2938
SchI GAGTC 4 cut(s) 201, 1127, 1179, 1746
ScrFI CCNGG 7 cut(s) 151, 410, 760, 774, 904, 976, 2314
SduI GDGCHC 3 cut(s) 780, 1432, 2322
SfaNI GCATC 6 cut(s) 1462, 1951, 2011, 2395, 2710, 2844
SfcI CTRYAG 1 cut(s) 1662
Sfr274I CTCGAG 1 cut(s) 2624
SfuI TTCGAA 2 cut(s) 745, 1535
SinI GGWCC 1 cut(s) 2886
SlaI CTCGAG 1 cut(s) 2624
SmiMI CAYNNNNRTG 3 cut(s) 2706, 2755, 2757
SmlI CTYRAG 4 cut(s) 104, 860, 1243, 2624
SmoI CTYRAG 4 cut(s) 104, 860, 1243, 2624
SphI GCATGC 1 cut(s) 64
SspI AATATT 1 cut(s) 1769
SspMI CTAG 6 cut(s) 1157, 2244, 2367, 2630, 2735, 2967
StyD4I CCNGG 7 cut(s) 149, 408, 758, 772, 902, 974, 2312
StyI CCWWGG 2 cut(s) 1058, 1716
TaaI ACNGT 7 cut(s) 28, 73, 1505, 2119, 2666, 2714, 2820
TaiI ACGT 6 cut(s) 662, 1143, 1524, 1564, 1568, 1783
TatI WGTACW 6 cut(s) 9, 1805, 2072, 2262, 2804, 2936
TauI GCSGC 1 cut(s) 473
Tru1I TTAA 4 cut(s) 1544, 2307, 2424, 2727
Tru9I TTAA 4 cut(s) 1544, 2307, 2424, 2727
TscAI CASTG 3 cut(s) 355, 388, 2235
TseFI GTSAC 3 cut(s) 346, 1215, 2708
TseI GCWGC 8 cut(s) 16, 19, 307, 1126, 1438, 2363, 2620, 2864
Tsp45I GTSAC 3 cut(s) 346, 1215, 2708
TspDTI ATGAA 9 cut(s) 546, 555, 659, 975, 1811, 2168, 2402, 2769, 2958
TspGWI ACGGA 1 cut(s) 101
TspRI CASTG 3 cut(s) 355, 388, 2235
Van91I CCANNNNNTGG 1 cut(s) 2011
VpaK11BI GGWCC 1 cut(s) 2886
XbaI TCTAGA 2 cut(s) 1156, 2734
XceI RCATGY 2 cut(s) 64, 1804
XcmI CCANNNNNNNNNTGG 1 cut(s) 1209
XhoI CTCGAG 1 cut(s) 2624
XmnI GAANNNNTTC 1 cut(s) 896
XspI CTAG 6 cut(s) 1157, 2244, 2367, 2630, 2735, 2967
ZrmI AGTACT 1 cut(s) 2938
Zsp2I ATGCAT 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.