Rw7G032530
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0009040
Physical Location & Seq
Reverse (-)
47306016 .. 47311336
5321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G032530.1

Sequence Viewer

Length: 2952 bp
ATGGCCAAAATACCAGTTCCCTCCTTGCACTTGCTACCGGTCTTTACCACATTGTTCATTTCTTTGCTTTTCCCGCCATGTATGCCTCTAACGGACGAAAGTGAAGCCCTCCTTGAGTTCAAAAGGCAGCTCAAGGACCCTTTGAGTTTTCTTGATTCGTGGAAAGAGTCGGAGTCTTCTTCCCCCTGTGAGTTCTCCGGGATTACTTGCGATTCGGGGAAAGTCACTGAAATATCTCTTCACAACAAGTCACTCTCAGGTGAGATTTCGCCCTCCATTGGCGTCCTTGAGAGCCTCACCACGCTTGTACTGTCTGCCAACCTCATTACAGGAGAGGTTCCTGCCCAACTGACTCATTGTGCCAATCTTAAAACATTGAATCTGTCTGAGAACCAATTGGTTGGAAAAATCCCGGACCTTTCCACCCTTAGAAACTTGGAGAGACTTGATCTTTCTGCCAACAGGTTATCTTCAGAGTTCCCGTCTTGGGTGGGAAATCTCACAGGTTTACAATATCTGGGCCTTGGGCAGAATCTGTTTGATGCAAGCGAAGTTCCTGGGAGTCTTGGAAACTTGAAGAACTTGACATGGCTGTATCTGACAAATTGCAGCTTGATGGGAGAGATTCCGGAATCTATTTACGAAATGAAGGCATTGGGAACATTTGATATGTCAAGAAACAAGCTTTCTGGGAAACTCTCTAAGTCGATTTCCAAGCTACAAAACCTATTTAAGATTGAGCTGTTTGCAAACGGTTTGGGTGGAGAAATCCCACCCGAGGTTGCTAATCTCACCCTTCTGAGGGAATTTGATATTTCAACAAACAGATTTTATGGGAAGTTGCCTTCAGAGTTTGGAAATCTCAAGAATTTAGTGGTTTTTCAGTTGTATGAAAACAATTTTTCGGGGGAATTCCCTGCTGGGTTTGGAGATATGCAGCATCTTACTGCTTTGTCAATATATGGAAACAGATTTACTGGGGAGTTTCCTTCAAATCTAGGGAAATTCTCACTTTTGGAGAGCATTGACATATCTGAGAATCAGTTTTCTGGTGCTTTCCCAAGGTTTCTTTGTGAAAAGAGGAAGCTGCAATTCTTGCTCGCTTTGGATAACAATTTTTATGGGGAGTTTCCAGGTTCTTATGCCGACTGTAAATCTCTAGAGAGGTTTAGAGTAAATAAGAACCATTTGTATGGGGAGATTCCAGCTGAGTTTTGGTCACTTCCTAAAGCGATAATCATTGATTTTAGTGACAATAACTTTCGTGGAGTGATTTCCCCCAGCATTGGGTTTTCTATCAGCTTGAATCTGTTGATGTTGCAGAACAACAATTTCTCAGGTTATCTTCCACCAGAAATTGGGAAGCTAACAAAGTTGGACAGGCTCTATTTGAATCATAATAACTTCTCAGGTGAAATACCTTCCGAAATTGGTGCCTTGAAGCAGTTATCATCTTTCCATGTTGAAGAAAATTCTTTGTCAGGCTCAATACCTTCAGAACTGGGAAACTGTGTTAGGTTGGCGGACATGAGTCTTGCTGGGAATTCCTTAACTGGAAGTATCCCACCTACGCTTTCACTCATGAGCTCTTTGAACTCTCTAAATCTCTCTTGGAATAAACTCTCAGGTGAGGTTCCAGAAAATTTAGAGAAATTGAAGCTAAGTTTCATAGATTTGTCTGAAAATCTATTATCTGGAAGAGTACCATCTGATCTTCTGACCACTGGTGGAGACAAAGCCTTTCGTGGAAACAAAGAACTCTGCATCGATCAATATTCAAAAACACATTCAAATTCTGGTATGAATACATGTTCTGAAAAGCATAGTCAGAAAAGGGTGCTTCAGAATAAATTGGTTTTGTTCTCTATCATAGCATCTGCCTTGGTTGTTGTTTTAGCTGGATTGATGCTTGTCAGTTATAAGAACTTCAAGCTTTCTGAGGTTGACAGAGAAAACAGTTTGGAAGAGGGGAAGGAAATAGATCCGAAATGGAAACTTGCATCTTTTCACCAACTGGAAATAGATGAAGATGAAATCTGTGATTTAGAAGAAGAAAATTTGGTTGGTAGTGGCAGCACCGGGAAAGTTTACCGCCTTGATCTGAAGAAGAATGGTGGTACAGTAGCTGTAAAACAACTATGGAAAGGCGATGGCTTGAAGCTTTTGACTGCAGAGATGGATATTTTGGGAAAGATCAGGCATAGAAATATATTGAAGCTTTATGCTTGTTTAGTGAAGGGAGGATCTAATCTTCTGGTGTTTGAGTACATGGAAAATGGAAATCTGTTTCAAGCGCTTCACAAACAGCTTAAAGGTGGGCAGCCGGAATTGGATTGGTACCAGAGGTATAGGATTGCTTTGGGAGCAGCTAGGGGGATTTCTTATCTACACCATGATTGTTCCCCACCTATACTTCATAGAGATATAAAATCAACCAACATTCTGCTTGATGATGATTACGAGGCCAAAGTTGCTGATTTTGGGGTTGCAAAAATTGTACAAAACACTCCCAAGGGTTCAGAGTGCAGCTCCTTTGCTGGGACACATGGGTATATTGCTCCTGAGCTAGCATATACTGCTAAAGTCACTGAAAAGTGCGATGTATATAGTTTCGGTGTGGTTCTACTTGAGCTAGTTACTGGTAGAAGACCAATTGAAGATGACTACGGGGAAGGAAAAGACATTGTGTACTGGGTTTCCGCCAATCTGAACGACCGTGAAAACGTTCTGAAGGTTCTTGATGATAAAGTGGCAGATGAAAATATCCAGGATGACATGATCAAGGTTTTGAAGGTAGCTATTCTTTGTACTACCAAGCTTCCATCCCTGCGGCCTACAATGCGAGAGGTGATCAAGATGCTTATTGATGCTGATCCTGGCACTTTCACGTCTCCAAGCAACAGTTCTGTCAAAGAAGGCAAGGATTCCTCCAGTCTGCTTCTCTCCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

983

Amino Acids

108.76

Weight (kDa)

5.39

Isoelectric Point (pI)

35.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 71 1.5e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 137 - 248 5.2e-08 Leucine-rich repeat region
LRR_8 PF13855 144 - 205 9.1e-09 Leucine rich repeat
LRR_14 PF23598 255 - 402 1.8e-09 Leucine-rich repeat region
LRR_14 PF23598 430 - 549 6.3e-09 Leucine-rich repeat region
Pkinase PF00069 683 - 952 3.6e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 685 - 954 5.4e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1920
Acc65I GGTACC 1 cut(s) 2337
AccB1I GGYRCC 2 cut(s) 1433, 2337
AccB7I CCANNNNNTGG 1 cut(s) 1358
AccIII TCCGGA 1 cut(s) 628
AciI CCGC 5 cut(s) 74, 1523, 2092, 2700, 2830
AclI AACGTT 1 cut(s) 2724
AclWI GGATC 3 cut(s) 1976, 2251, 2867
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 9 cut(s) 806, 868, 911, 1004, 1471, 1543, 1642, 1792, 2056
AcuI CTGAAG 6 cut(s) 456, 831, 1479, 1826, 2123, 2750
AcyI GRCGYC 1 cut(s) 282
AfaI GTAC 8 cut(s) 309, 1704, 2119, 2267, 2339, 2499, 2690, 2809
AfeI AGCGCT 1 cut(s) 2295
AfiI CCNNNNNNNGG 9 cut(s) 278, 487, 778, 801, 802, 1286, 1287, 1358, 2538
AflIII ACRYGT 1 cut(s) 1808
AgeI ACCGGT 1 cut(s) 37
AjiI CACGTC 1 cut(s) 2889
AjnI CCWGG 4 cut(s) 556, 1132, 2766, 2875
AjuI GAANNNNNNNTTGG 4 cut(s) 2046, 2078, 2887, 2919
Alw21I GWGCWC 1 cut(s) 1589
Alw26I GTCTC 3 cut(s) 436, 1725, 2895
AlwI GGATC 3 cut(s) 1976, 2251, 2867
AlwNI CAGNNNCTG 2 cut(s) 535, 2126
Ama87I CYCGRG 1 cut(s) 776
Aor13HI TCCGGA 1 cut(s) 628
Aor51HI AGCGCT 1 cut(s) 2295
AoxI GGCC 4 cut(s) 3, 520, 2463, 2831
ApeKI GCWGC 8 cut(s) 127, 609, 937, 1087, 2073, 2320, 2366, 2526
ApoI RAATTY 9 cut(s) 806, 868, 911, 1004, 1471, 1543, 1642, 1792, 2056
AsiGI ACCGGT 1 cut(s) 37
Asp700I GAANNNNTTC 1 cut(s) 2724
Asp718I GGTACC 1 cut(s) 2337
AspLEI GCGC 1 cut(s) 2296
AspS9I GGNCC 3 cut(s) 136, 415, 520
AsuC2I CCSGG 3 cut(s) 199, 413, 2080
AsuHPI GGTGA 7 cut(s) 272, 289, 784, 1424, 1640, 2000, 2860
AsuNHI GCTAGC 1 cut(s) 2566
AvaI CYCGRG 1 cut(s) 776
AvaII GGWCC 2 cut(s) 136, 415
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 1433, 2337
BanII GRGCYC 1 cut(s) 1589
BbsI GAAGAC 2 cut(s) 168, 2653
Bbv12I GWGCWC 1 cut(s) 1589
BbvI GCAGC 8 cut(s) 139, 621, 949, 1074, 2085, 2332, 2378, 2538
BccI CCATC 5 cut(s) 610, 1714, 2144, 2170, 2830
BcgI CGANNNNNNTGC 2 cut(s) 1415, 1449
BciT130I CCWGG 4 cut(s) 558, 1134, 2768, 2877
BciVI GTATCC 1 cut(s) 1571
BclI TGATCA 2 cut(s) 2778, 2850
BcnI CCSGG 3 cut(s) 199, 413, 2080
BcoDI GTCTC 3 cut(s) 436, 1725, 2895
BfaI CTAG 5 cut(s) 998, 1160, 2370, 2567, 2633
BfmI CTRYAG 1 cut(s) 2169
BfoI RGCGCY 1 cut(s) 2297
BfuI GTATCC 1 cut(s) 1571
BisI GCNGC 9 cut(s) 128, 610, 938, 1088, 2074, 2321, 2367, 2527, 2831
BlsI GCNGC 9 cut(s) 129, 611, 939, 1089, 2075, 2322, 2368, 2528, 2832
Bme1390I CCNGG 7 cut(s) 199, 413, 558, 1134, 2080, 2768, 2877
Bme18I GGWCC 2 cut(s) 136, 415
BmeT110I CYCGRG 1 cut(s) 776
BmgBI CACGTC 1 cut(s) 2889
BmgT120I GGNCC 3 cut(s) 136, 415, 520
BmiI GGNNCC 5 cut(s) 138, 339, 1435, 1635, 2339
BmrFI CCNGG 7 cut(s) 199, 413, 558, 1134, 2080, 2768, 2877
BmrI ACTGGG 4 cut(s) 987, 1511, 2701, 2941
BmsI GCATC 8 cut(s) 532, 949, 1773, 1883, 1896, 2009, 2847, 2857
BmtI GCTAGC 1 cut(s) 2570
BmuI ACTGGG 4 cut(s) 987, 1511, 2701, 2941
BoxI GACNNNNGTC 1 cut(s) 1530
BpiI GAAGAC 2 cut(s) 168, 2653
BplI GAGNNNNNCTC 2 cut(s) 2513, 2545
BpmI CTGGAG 1 cut(s) 2914
Bpu10I CCTNAGC 1 cut(s) 2562
BpuEI CTTGAG 5 cut(s) 116, 134, 308, 848, 2648
BpuMI CCSGG 3 cut(s) 199, 413, 2080
Bsa29I ATCGAT 1 cut(s) 1767
BsaBI GATNNNNATC 1 cut(s) 2871
BsaHI GRCGYC 1 cut(s) 282
BsaJI CCNNGG 6 cut(s) 523, 557, 777, 1061, 1881, 2511
BsaWI WCCGGW 2 cut(s) 37, 628
Bsc4I CCNNNNNNNGG 9 cut(s) 278, 487, 778, 801, 802, 1286, 1287, 1358, 2538
Bse118I RCCGGY 1 cut(s) 37
Bse8I GATNNNNATC 1 cut(s) 2871
BseAI TCCGGA 1 cut(s) 628
BseBI CCWGG 4 cut(s) 558, 1134, 2768, 2877
BseCI ATCGAT 1 cut(s) 1767
BseDI CCNNGG 6 cut(s) 523, 557, 777, 1061, 1881, 2511
BseGI GGATG 2 cut(s) 2776, 2822
BseJI GATNNNNATC 1 cut(s) 2871
BseLI CCNNNNNNNGG 9 cut(s) 278, 487, 778, 801, 802, 1286, 1287, 1358, 2538
BseXI GCAGC 8 cut(s) 139, 621, 949, 1074, 2085, 2332, 2378, 2538
BseYI CCCAGC 4 cut(s) 920, 1280, 1538, 2537
BsgI GTGCAG 1 cut(s) 2545
Bsh1285I CGRYCG 1 cut(s) 2716
BshFI GGCC 4 cut(s) 5, 522, 2465, 2833
BshNI GGYRCC 2 cut(s) 1433, 2337
BshTI ACCGGT 1 cut(s) 37
BshVI ATCGAT 1 cut(s) 1767
BsiEI CGRYCG 1 cut(s) 2716
BsiHKAI GWGCWC 1 cut(s) 1589
BsiHKCI CYCGRG 1 cut(s) 776
BsiSI CCGG 6 cut(s) 38, 198, 413, 629, 2079, 2324
BslFI GGGAC 1 cut(s) 2554
BslI CCNNNNNNNGG 9 cut(s) 278, 487, 778, 801, 802, 1286, 1287, 1358, 2538
BsmAI GTCTC 3 cut(s) 436, 1725, 2895
BsmBI CGTCTC 1 cut(s) 2895
BsmFI GGGAC 1 cut(s) 2554
BsnI GGCC 4 cut(s) 5, 522, 2465, 2833
BsoBI CYCGRG 1 cut(s) 776
Bsp1286I GDGCHC 1 cut(s) 1589
Bsp13I TCCGGA 1 cut(s) 628
Bsp1407I TGTACA 1 cut(s) 2497
BspACI CCGC 5 cut(s) 74, 1523, 2092, 2700, 2830
BspANI GGCC 4 cut(s) 5, 522, 2465, 2833
BspDI ATCGAT 1 cut(s) 1767
BspEI TCCGGA 1 cut(s) 628
BspHI TCATGA 1 cut(s) 1581
BspLI GGNNCC 5 cut(s) 138, 339, 1435, 1635, 2339
BspMAI CTGCAG 1 cut(s) 2173
BspOI GCTAGC 1 cut(s) 2570
BspPI GGATC 3 cut(s) 1976, 2251, 2867
BspT107I GGYRCC 2 cut(s) 1433, 2337
BsrFI RCCGGY 1 cut(s) 37
BsrGI TGTACA 1 cut(s) 2497
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 6 cut(s) 523, 557, 777, 1061, 1881, 2511
BssNI GRCGYC 1 cut(s) 282
BssT1I CCWWGG 4 cut(s) 523, 1061, 1881, 2511
Bst2UI CCWGG 4 cut(s) 558, 1134, 2768, 2877
Bst4CI ACNGT 8 cut(s) 312, 755, 1151, 1511, 1958, 2122, 2717, 2903
Bst6I CTCTTC 3 cut(s) 243, 1693, 1959
BstACI GRCGYC 1 cut(s) 282
BstAPI GCANNNNNTGC 2 cut(s) 1096, 2576
BstAUI TGTACA 1 cut(s) 2497
BstC8I GCNNGC 3 cut(s) 547, 1101, 2568
BstF5I GGATG 2 cut(s) 2776, 2822
BstH2I RGCGCY 1 cut(s) 2297
BstHHI GCGC 1 cut(s) 2296
BstMAI GTCTC 3 cut(s) 436, 1725, 2895
BstMCI CGRYCG 1 cut(s) 2716
BstMWI GCNNNNNNNGC 7 cut(s) 73, 82, 1096, 2363, 2471, 2576, 2839
BstNI CCWGG 4 cut(s) 558, 1134, 2768, 2877
BstNSI RCATGY 1 cut(s) 1812
BstPAI GACNNNNGTC 1 cut(s) 1530
BstSCI CCNGG 7 cut(s) 197, 411, 556, 1132, 2078, 2766, 2875
BstSFI CTRYAG 1 cut(s) 2169
BstV1I GCAGC 8 cut(s) 139, 621, 949, 1074, 2085, 2332, 2378, 2538
BstV2I GAAGAC 2 cut(s) 168, 2653
BstX2I RGATCY 2 cut(s) 1981, 2243
BstXI CCANNNNNNTGG 2 cut(s) 401, 1193
BstYI RGATCY 2 cut(s) 1981, 2243
Bsu15I ATCGAT 1 cut(s) 1767
BsuI GTATCC 1 cut(s) 1571
BsuRI GGCC 4 cut(s) 5, 522, 2465, 2833
BsuTUI ATCGAT 1 cut(s) 1767
BtgZI GCGATG 2 cut(s) 2163, 2613
BtrI CACGTC 1 cut(s) 2889
BtsCI GGATG 2 cut(s) 2776, 2822
BtsIMutI CAGTG 3 cut(s) 225, 1722, 2586
Cac8I GCNNGC 3 cut(s) 547, 1101, 2568
CaiI CAGNNNCTG 2 cut(s) 535, 2126
CciI TCATGA 1 cut(s) 1581
CfoI GCGC 1 cut(s) 2296
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 3 cut(s) 136, 415, 520
ClaI ATCGAT 1 cut(s) 1767
CseI GACGC 1 cut(s) 271
Csp6I GTAC 8 cut(s) 308, 1703, 2118, 2266, 2338, 2498, 2689, 2808
CspAI ACCGGT 1 cut(s) 37
CviQI GTAC 8 cut(s) 308, 1703, 2118, 2266, 2338, 2498, 2689, 2808
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 243, 1693, 1959
EarI CTCTTC 3 cut(s) 243, 1693, 1959
EciI GGCGGA 2 cut(s) 1538, 2689
Ecl136II GAGCTC 1 cut(s) 1587
Eco130I CCWWGG 4 cut(s) 523, 1061, 1881, 2511
Eco24I GRGCYC 1 cut(s) 1589
Eco47I GGWCC 2 cut(s) 136, 415
Eco47III AGCGCT 1 cut(s) 2295
Eco53kI GAGCTC 1 cut(s) 1587
Eco57I CTGAAG 6 cut(s) 456, 831, 1479, 1826, 2123, 2750
Eco88I CYCGRG 1 cut(s) 776
EcoICRI GAGCTC 1 cut(s) 1587
EcoO109I RGGNCCY 1 cut(s) 136
EcoRI GAATTC 2 cut(s) 911, 1543
EcoRII CCWGG 4 cut(s) 556, 1132, 2766, 2875
EcoT14I CCWWGG 4 cut(s) 523, 1061, 1881, 2511
EcoT38I GRGCYC 1 cut(s) 1589
ErhI CCWWGG 4 cut(s) 523, 1061, 1881, 2511
Esp3I CGTCTC 1 cut(s) 2895
FalI AAGNNNNNCTT 2 cut(s) 96, 128
FaqI GGGAC 1 cut(s) 2554
FauI CCCGC 1 cut(s) 81
FbaI TGATCA 2 cut(s) 2778, 2850
Fnu4HI GCNGC 9 cut(s) 128, 610, 938, 1088, 2074, 2321, 2367, 2527, 2831
FokI GGATG 2 cut(s) 2783, 2809
FriOI GRGCYC 1 cut(s) 1589
Fsp4HI GCNGC 9 cut(s) 128, 610, 938, 1088, 2074, 2321, 2367, 2527, 2831
FspBI CTAG 5 cut(s) 998, 1160, 2370, 2567, 2633
GlaI GCGC 1 cut(s) 2295
GluI GCNGC 9 cut(s) 128, 610, 938, 1088, 2074, 2321, 2367, 2527, 2831
GsaI CCCAGC 4 cut(s) 924, 1284, 1542, 2541
GsuI CTGGAG 1 cut(s) 2914
HaeII RGCGCY 1 cut(s) 2297
HaeIII GGCC 4 cut(s) 5, 522, 2465, 2833
HapII CCGG 6 cut(s) 38, 198, 413, 629, 2079, 2324
HgaI GACGC 1 cut(s) 271
HhaI GCGC 1 cut(s) 2296
Hin1I GRCGYC 1 cut(s) 282
Hin6I GCGC 1 cut(s) 2294
HinP1I GCGC 1 cut(s) 2294
HincII GTYRAC 1 cut(s) 1945
HindII GTYRAC 1 cut(s) 1945
HindIII AAGCTT 5 cut(s) 683, 1931, 2159, 2216, 2816
HpaII CCGG 6 cut(s) 38, 198, 413, 629, 2079, 2324
HphI GGTGA 7 cut(s) 272, 289, 784, 1424, 1640, 2000, 2860
Hpy166II GTNNAC 4 cut(s) 509, 1945, 2089, 2689
Hpy8I GTNNAC 4 cut(s) 509, 1945, 2089, 2689
HpyCH4III ACNGT 8 cut(s) 312, 755, 1151, 1511, 1958, 2122, 2717, 2903
HpyCH4IV ACGT 2 cut(s) 2724, 2888
HpyF10VI GCNNNNNNNGC 7 cut(s) 73, 82, 1096, 2363, 2471, 2576, 2839
HpySE526I ACGT 2 cut(s) 2724, 2888
Hsp92I GRCGYC 1 cut(s) 282
HspAI GCGC 1 cut(s) 2294
Kpn2I TCCGGA 1 cut(s) 628
KpnI GGTACC 1 cut(s) 2341
Ksp22I TGATCA 2 cut(s) 2778, 2850
LmnI GCTCC 3 cut(s) 2363, 2534, 2563
Lsp1109I GCAGC 8 cut(s) 139, 621, 949, 1074, 2085, 2332, 2378, 2538
LweI GCATC 8 cut(s) 532, 949, 1773, 1883, 1896, 2009, 2847, 2857
MaeI CTAG 5 cut(s) 998, 1160, 2370, 2567, 2633
MaeII ACGT 2 cut(s) 2724, 2888
MaeIII GTNAC 6 cut(s) 223, 249, 1218, 1250, 2584, 2635
MfeI CAATTG 2 cut(s) 395, 2652
MflI RGATCY 2 cut(s) 1981, 2243
MhlI GDGCHC 1 cut(s) 1589
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 5 cut(s) 176, 182, 346, 571, 1540
MmeI TCCRAC 3 cut(s) 150, 382, 1356
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 628
MroXI GAANNNNTTC 1 cut(s) 2724
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 369, 732, 1550, 2310
MslI CAYNNNNRTG 1 cut(s) 1191
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 1208
MspI CCGG 6 cut(s) 38, 198, 413, 629, 2079, 2324
MspR9I CCNGG 7 cut(s) 199, 413, 558, 1134, 2080, 2768, 2877
MunI CAATTG 2 cut(s) 395, 2652
MvaI CCWGG 4 cut(s) 558, 1134, 2768, 2877
MwoI GCNNNNNNNGC 7 cut(s) 73, 82, 1096, 2363, 2471, 2576, 2839
NciI CCSGG 3 cut(s) 199, 413, 2080
NheI GCTAGC 1 cut(s) 2566
NlaIV GGNNCC 5 cut(s) 138, 339, 1435, 1635, 2339
NmuCI GTSAC 5 cut(s) 223, 249, 1218, 1250, 2584
NspI RCATGY 1 cut(s) 1812
PagI TCATGA 1 cut(s) 1581
PciI ACATGT 1 cut(s) 1808
PdmI GAANNNNTTC 1 cut(s) 2724
PflMI CCANNNNNTGG 1 cut(s) 1358
PfoI TCCNGGA 3 cut(s) 197, 411, 2766
PinAI ACCGGT 1 cut(s) 37
PkrI GCNGC 9 cut(s) 129, 611, 939, 1089, 2075, 2322, 2368, 2528, 2832
PleI GAGTC 5 cut(s) 175, 181, 346, 570, 1539
PpsI GAGTC 5 cut(s) 175, 181, 346, 570, 1539
PpuMI RGGWCCY 1 cut(s) 136
PscI ACATGT 1 cut(s) 1808
PshAI GACNNNNGTC 1 cut(s) 1530
PsiI TTATAA 1 cut(s) 1920
Psp124BI GAGCTC 1 cut(s) 1589
Psp1406I AACGTT 1 cut(s) 2724
Psp5II RGGWCCY 1 cut(s) 136
Psp6I CCWGG 4 cut(s) 556, 1132, 2766, 2875
PspFI CCCAGC 4 cut(s) 920, 1280, 1538, 2537
PspGI CCWGG 4 cut(s) 556, 1132, 2766, 2875
PspN4I GGNNCC 5 cut(s) 138, 339, 1435, 1635, 2339
PspPI GGNCC 3 cut(s) 136, 415, 520
PspPPI RGGWCCY 1 cut(s) 136
PstI CTGCAG 1 cut(s) 2173
PstNI CAGNNNCTG 2 cut(s) 535, 2126
PsuI RGATCY 2 cut(s) 1981, 2243
PvuII CAGCTG 1 cut(s) 1208
RsaI GTAC 8 cut(s) 309, 1704, 2119, 2267, 2339, 2499, 2690, 2809
RsaNI GTAC 8 cut(s) 308, 1703, 2118, 2266, 2338, 2498, 2689, 2808
RseI CAYNNNNRTG 1 cut(s) 1191
SacI GAGCTC 1 cut(s) 1589
SaqAI TTAA 4 cut(s) 369, 732, 1550, 2310
SatI GCNGC 9 cut(s) 128, 610, 938, 1088, 2074, 2321, 2367, 2527, 2831
Sau96I GGNCC 3 cut(s) 136, 415, 520
SchI GAGTC 5 cut(s) 176, 182, 346, 571, 1540
ScrFI CCNGG 7 cut(s) 199, 413, 558, 1134, 2080, 2768, 2877
SduI GDGCHC 1 cut(s) 1589
SfaNI GCATC 8 cut(s) 532, 949, 1773, 1883, 1896, 2009, 2847, 2857
SfcI CTRYAG 1 cut(s) 2169
SinI GGWCC 2 cut(s) 136, 415
SmiMI CAYNNNNRTG 1 cut(s) 1191
SmlI CTYRAG 5 cut(s) 113, 131, 287, 863, 2627
SmoI CTYRAG 5 cut(s) 113, 131, 287, 863, 2627
SsiI CCGC 5 cut(s) 74, 1523, 2092, 2700, 2830
SspI AATATT 1 cut(s) 1775
SspMI CTAG 5 cut(s) 998, 1160, 2370, 2567, 2633
SstI GAGCTC 1 cut(s) 1589
StyD4I CCNGG 7 cut(s) 197, 411, 556, 1132, 2078, 2766, 2875
StyI CCWWGG 4 cut(s) 523, 1061, 1881, 2511
TaaI ACNGT 8 cut(s) 312, 755, 1151, 1511, 1958, 2122, 2717, 2903
TaiI ACGT 2 cut(s) 2727, 2891
TaqI TCGA 2 cut(s) 707, 1767
TatI WGTACW 5 cut(s) 307, 2265, 2497, 2688, 2807
TauI GCSGC 1 cut(s) 2833
Tru1I TTAA 4 cut(s) 369, 732, 1550, 2310
Tru9I TTAA 4 cut(s) 369, 732, 1550, 2310
TscAI CASTG 3 cut(s) 232, 1729, 2593
TseFI GTSAC 5 cut(s) 223, 249, 1218, 1250, 2584
TseI GCWGC 8 cut(s) 127, 609, 937, 1087, 2073, 2320, 2366, 2526
Tsp45I GTSAC 5 cut(s) 223, 249, 1218, 1250, 2584
TspDTI ATGAA 9 cut(s) 46, 662, 906, 1657, 1817, 2040, 2046, 2405, 2772
TspGWI ACGGA 1 cut(s) 107
TspRI CASTG 3 cut(s) 232, 1729, 2593
Van91I CCANNNNNTGG 1 cut(s) 1358
VpaK11BI GGWCC 2 cut(s) 136, 415
XapI RAATTY 9 cut(s) 806, 868, 911, 1004, 1471, 1543, 1642, 1792, 2056
XbaI TCTAGA 1 cut(s) 1159
XceI RCATGY 1 cut(s) 1812
XcmI CCANNNNNNNNNTGG 1 cut(s) 1212
XmnI GAANNNNTTC 1 cut(s) 2724
XspI CTAG 5 cut(s) 998, 1160, 2370, 2567, 2633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.