MD15G1404900.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009040
Physical Location & Seq
Reverse (-)
50577372 .. 50578637
1266 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1404900.v1.1.491

Sequence Viewer

Length: 807 bp
ATGAAGCTTTTGATGGCAGAGATGGACATTTTGGGGAAGATAAGGCATAAAAATATACTGAAGCTATACGCTTGTTTAGTGAAGGGCGGATCTAGAGTTCTCGTGTTTGAGTACATGGCAAATGGTAATTTGTTTGAAGCGCTTCACAGAGAGATTAAATGCGGGAAGCCCGAATTGGATTGGTGCCAGAGGTATAAGATTGCTTTGGGAGCAGCTCGGGGGATTGCTTATCTGCACCATGATTGTTCACCAGCTATAATTCACAGGGATATTAAATCCACCAACATTCTGCTCGACGACGATTATGAGGCAAAAGTTGCTGATTTTGGGGTTGCAAAGATTGCAGAAAATTCTCAGAAGGGTTCTGATTACAGCTCATTCGCCGGCACACATGGCTATATTGCTCCCGAGCTGGCATATACTGCTAAAGTGAACGAAAAGTGCGATGTGTATAGCTTCGGTGTTGTGCTGCTAGAGCTAGTAACTGGTAGGAGACCAATTGAAGAGGATTACGGGGAAGGAAAAGATATTGTTTACTGGGTTTCAACGCATCTGAGTGACCGTGAAGCGGTTGTTAAGATTCTCGATGAAAAAGTGGCAGATGTAAGTGTCCAGGATGACATGATCAAGGTCTTGAAGGTTGCTGTTCTTTGTACTACGAAGCTACCATCGTTGCGCCCGACAATGCGGGAGGTTATAAAGATGCTTGCGGATGCTGATCCTTGCAGTTTCCGGTCCGGGAACAATACCGGTAACGACAAAAAAGTCAAGGATGTCTCACCCGGTACCGATGAGTTGCTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

269

Amino Acids

29.85

Weight (kDa)

6.19

Isoelectric Point (pI)

28.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 234 7.5e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 4 - 236 4.7e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 698
AasI GACNNNNNNGTC 1 cut(s) 764
Acc65I GGTACC 1 cut(s) 785
AccB1I GGYRCC 2 cut(s) 183, 785
AciI CCGC 5 cut(s) 87, 162, 569, 688, 710
AclWI GGATC 2 cut(s) 97, 713
AcsI RAATTY 1 cut(s) 349
AcuI CTGAAG 1 cut(s) 80
AfaI GTAC 3 cut(s) 113, 655, 787
AfeI AGCGCT 1 cut(s) 141
AfiI CCNNNNNNNGG 1 cut(s) 568
AgeI ACCGGT 1 cut(s) 749
AgsI TTSAA 4 cut(s) 137, 503, 546, 637
AjnI CCWGG 1 cut(s) 612
AjuI GAANNNNNNNTTGG 2 cut(s) 158, 190
AloI GAACNNNNNNTCC 2 cut(s) 81, 113
AluBI AGCT 9 cut(s) 7, 64, 215, 254, 375, 412, 456, 478, 664
AluI AGCT 9 cut(s) 7, 64, 215, 254, 375, 412, 456, 478, 664
Alw26I GTCTC 2 cut(s) 487, 781
AlwI GGATC 2 cut(s) 97, 713
Ama87I CYCGRG 2 cut(s) 216, 407
Aor51HI AGCGCT 1 cut(s) 141
ApeKI GCWGC 2 cut(s) 212, 469
ApoI RAATTY 1 cut(s) 349
AsiGI ACCGGT 1 cut(s) 749
Asp700I GAANNNNTTC 1 cut(s) 141
Asp718I GGTACC 1 cut(s) 785
AspLEI GCGC 2 cut(s) 142, 678
AspS9I GGNCC 1 cut(s) 735
AsuC2I CCSGG 2 cut(s) 739, 783
AsuHPI GGTGA 2 cut(s) 240, 771
AvaI CYCGRG 2 cut(s) 216, 407
AvaII GGWCC 1 cut(s) 735
BanI GGYRCC 2 cut(s) 183, 785
BauI CACGAG 1 cut(s) 101
BbvI GCAGC 2 cut(s) 224, 456
BccI CCATC 3 cut(s) 7, 16, 676
BciT130I CCWGG 1 cut(s) 614
BclI TGATCA 1 cut(s) 624
BcnI CCSGG 2 cut(s) 739, 783
BcoDI GTCTC 2 cut(s) 487, 781
BfaI CTAG 3 cut(s) 93, 473, 479
BfoI RGCGCY 1 cut(s) 143
BisI GCNGC 2 cut(s) 213, 470
BlsI GCNGC 2 cut(s) 214, 471
Bme1390I CCNGG 3 cut(s) 614, 739, 783
Bme18I GGWCC 1 cut(s) 735
BmeT110I CYCGRG 2 cut(s) 216, 407
BmgT120I GGNCC 1 cut(s) 735
BmiI GGNNCC 2 cut(s) 185, 787
BmrFI CCNGG 3 cut(s) 614, 739, 783
BmrI ACTGGG 1 cut(s) 547
BmsI GCATC 3 cut(s) 559, 693, 703
BmuI ACTGGG 1 cut(s) 547
BpuMI CCSGG 2 cut(s) 739, 783
BsaBI GATNNNNATC 1 cut(s) 717
BsaI GGTCTC 1 cut(s) 487
BsaWI WCCGGW 2 cut(s) 732, 749
Bsc4I CCNNNNNNNGG 1 cut(s) 568
Bse118I RCCGGY 2 cut(s) 383, 749
Bse1I ACTGG 2 cut(s) 490, 542
Bse8I GATNNNNATC 1 cut(s) 717
BseBI CCWGG 1 cut(s) 614
BseGI GGATG 3 cut(s) 622, 718, 778
BseJI GATNNNNATC 1 cut(s) 717
BseLI CCNNNNNNNGG 1 cut(s) 568
BseMII CTCAG 2 cut(s) 368, 545
BseNI ACTGG 2 cut(s) 490, 542
BseXI GCAGC 2 cut(s) 224, 456
BsgI GTGCAG 1 cut(s) 218
BshNI GGYRCC 2 cut(s) 183, 785
BshTI ACCGGT 1 cut(s) 749
BsiHKCI CYCGRG 2 cut(s) 216, 407
BsiSI CCGG 5 cut(s) 384, 733, 738, 750, 783
BslI CCNNNNNNNGG 1 cut(s) 568
BsmAI GTCTC 2 cut(s) 487, 781
Bso31I GGTCTC 1 cut(s) 487
BsoBI CYCGRG 2 cut(s) 216, 407
Bsp143I GATC 3 cut(s) 89, 624, 718
BspACI CCGC 5 cut(s) 87, 162, 569, 688, 710
BspCNI CTCAG 2 cut(s) 367, 546
BspLI GGNNCC 2 cut(s) 185, 787
BspPI GGATC 2 cut(s) 97, 713
BspT107I GGYRCC 2 cut(s) 183, 785
BspTNI GGTCTC 1 cut(s) 487
BsrFI RCCGGY 2 cut(s) 383, 749
BsrI ACTGG 2 cut(s) 490, 542
BssAI RCCGGY 2 cut(s) 383, 749
BssMI GATC 3 cut(s) 89, 624, 718
BssSI CACGAG 1 cut(s) 101
Bst2BI CACGAG 1 cut(s) 101
Bst2UI CCWGG 1 cut(s) 614
Bst4CI ACNGT 1 cut(s) 563
Bst6I CTCTTC 1 cut(s) 498
BstAPI GCANNNNNTGC 3 cut(s) 317, 341, 422
BstC8I GCNNGC 3 cut(s) 385, 414, 708
BstDEI CTNAG 2 cut(s) 354, 554
BstF5I GGATG 3 cut(s) 622, 718, 778
BstH2I RGCGCY 1 cut(s) 143
BstHHI GCGC 2 cut(s) 142, 678
BstKTI GATC 3 cut(s) 92, 627, 721
BstMAI GTCTC 2 cut(s) 487, 781
BstMBI GATC 3 cut(s) 89, 624, 718
BstMWI GCNNNNNNNGC 6 cut(s) 209, 317, 341, 393, 422, 475
BstNI CCWGG 1 cut(s) 614
BstSCI CCNGG 3 cut(s) 612, 737, 781
BstV1I GCAGC 2 cut(s) 224, 456
BstX2I RGATCY 1 cut(s) 89
BstYI RGATCY 1 cut(s) 89
BtgZI GCGATG 1 cut(s) 459
BtsCI GGATG 3 cut(s) 622, 718, 778
Cac8I GCNNGC 3 cut(s) 385, 414, 708
CfoI GCGC 2 cut(s) 142, 678
Cfr10I RCCGGY 2 cut(s) 383, 749
Cfr13I GGNCC 1 cut(s) 735
CpoI CGGWCCG 1 cut(s) 735
Csp6I GTAC 3 cut(s) 112, 654, 786
CspAI ACCGGT 1 cut(s) 749
CspI CGGWCCG 1 cut(s) 735
CviAII CATG 4 cut(s) 115, 239, 392, 622
CviQI GTAC 3 cut(s) 112, 654, 786
DdeI CTNAG 2 cut(s) 354, 554
DpnI GATC 3 cut(s) 91, 626, 720
DpnII GATC 3 cut(s) 89, 624, 718
DrdI GACNNNNNNGTC 1 cut(s) 764
DseDI GACNNNNNNGTC 1 cut(s) 764
Eam1104I CTCTTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 498
EciI GGCGGA 1 cut(s) 102
Eco31I GGTCTC 1 cut(s) 487
Eco47I GGWCC 1 cut(s) 735
Eco47III AGCGCT 1 cut(s) 141
Eco57I CTGAAG 1 cut(s) 80
Eco88I CYCGRG 2 cut(s) 216, 407
EcoRII CCWGG 1 cut(s) 612
FaeI CATG 4 cut(s) 118, 242, 395, 625
FatI CATG 4 cut(s) 114, 238, 391, 621
FauI CCCGC 2 cut(s) 155, 681
FbaI TGATCA 1 cut(s) 624
Fnu4HI GCNGC 2 cut(s) 213, 470
FokI GGATG 3 cut(s) 629, 725, 785
Fsp4HI GCNGC 2 cut(s) 213, 470
FspBI CTAG 3 cut(s) 93, 473, 479
GlaI GCGC 2 cut(s) 141, 677
GluI GCNGC 2 cut(s) 213, 470
HaeII RGCGCY 1 cut(s) 143
HapII CCGG 5 cut(s) 384, 733, 738, 750, 783
HhaI GCGC 2 cut(s) 142, 678
Hin1II CATG 4 cut(s) 118, 242, 395, 625
Hin6I GCGC 2 cut(s) 140, 676
HinP1I GCGC 2 cut(s) 140, 676
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 1 cut(s) 580
HpaII CCGG 5 cut(s) 384, 733, 738, 750, 783
HphI GGTGA 2 cut(s) 240, 771
Hpy166II GTNNAC 3 cut(s) 248, 433, 535
Hpy188I TCNGA 3 cut(s) 357, 367, 555
Hpy188III TCNNGA 4 cut(s) 93, 407, 584, 634
Hpy8I GTNNAC 3 cut(s) 248, 433, 535
Hpy99I CGWCG 2 cut(s) 299, 302
HpyAV CCTTC 4 cut(s) 76, 352, 512, 631
HpyCH4III ACNGT 1 cut(s) 563
HpyCH4V TGCA 4 cut(s) 235, 335, 344, 726
HpyF10VI GCNNNNNNNGC 6 cut(s) 209, 317, 341, 393, 422, 475
HpyF3I CTNAG 2 cut(s) 354, 554
Hsp92II CATG 4 cut(s) 118, 242, 395, 625
HspAI GCGC 2 cut(s) 140, 676
KpnI GGTACC 1 cut(s) 789
KroI GCCGGC 1 cut(s) 383
KroNI GCCGGC 1 cut(s) 385
Ksp22I TGATCA 1 cut(s) 624
Kzo9I GATC 3 cut(s) 89, 624, 718
LmnI GCTCC 2 cut(s) 209, 409
Lsp1109I GCAGC 2 cut(s) 224, 456
LweI GCATC 3 cut(s) 559, 693, 703
MaeI CTAG 3 cut(s) 93, 473, 479
MaeIII GTNAC 3 cut(s) 481, 557, 752
MalI GATC 3 cut(s) 91, 626, 720
MboI GATC 3 cut(s) 89, 624, 718
MboII GAAGA 2 cut(s) 49, 515
MfeI CAATTG 1 cut(s) 498
MflI RGATCY 1 cut(s) 89
MluCI AATT 5 cut(s) 127, 173, 258, 349, 498
MnlI CCTC 4 cut(s) 183, 301, 499, 685
MroNI GCCGGC 1 cut(s) 383
MroXI GAANNNNTTC 1 cut(s) 141
MseI TTAA 3 cut(s) 156, 273, 576
MslI CAYNNNNRTG 1 cut(s) 555
MspI CCGG 5 cut(s) 384, 733, 738, 750, 783
MspR9I CCNGG 3 cut(s) 614, 739, 783
MunI CAATTG 1 cut(s) 498
MvaI CCWGG 1 cut(s) 614
MwoI GCNNNNNNNGC 6 cut(s) 209, 317, 341, 393, 422, 475
NaeI GCCGGC 1 cut(s) 385
NciI CCSGG 2 cut(s) 739, 783
NdeII GATC 3 cut(s) 89, 624, 718
NgoMIV GCCGGC 1 cut(s) 383
NlaIII CATG 4 cut(s) 118, 242, 395, 625
NlaIV GGNNCC 2 cut(s) 185, 787
NmuCI GTSAC 1 cut(s) 557
PcsI WCGNNNNNNNCGW 2 cut(s) 441, 677
PdiI GCCGGC 1 cut(s) 385
PdmI GAANNNNTTC 1 cut(s) 141
PfeI GAWTC 1 cut(s) 580
PfoI TCCNGGA 2 cut(s) 612, 737
PinAI ACCGGT 1 cut(s) 749
PkrI GCNGC 2 cut(s) 214, 471
PsiI TTATAA 1 cut(s) 698
Psp6I CCWGG 1 cut(s) 612
PspGI CCWGG 1 cut(s) 612
PspN4I GGNNCC 2 cut(s) 185, 787
PspPI GGNCC 1 cut(s) 735
PsuI RGATCY 1 cut(s) 89
RsaI GTAC 3 cut(s) 113, 655, 787
RsaNI GTAC 3 cut(s) 112, 654, 786
RseI CAYNNNNRTG 1 cut(s) 555
Rsr2I CGGWCCG 1 cut(s) 735
RsrII CGGWCCG 1 cut(s) 735
SaqAI TTAA 3 cut(s) 156, 273, 576
SatI GCNGC 2 cut(s) 213, 470
Sau3AI GATC 3 cut(s) 89, 624, 718
Sau96I GGNCC 1 cut(s) 735
ScrFI CCNGG 3 cut(s) 614, 739, 783
SfaNI GCATC 3 cut(s) 559, 693, 703
SinI GGWCC 1 cut(s) 735
SmiMI CAYNNNNRTG 1 cut(s) 555
Sse9I AATT 5 cut(s) 127, 173, 258, 349, 498
SsiI CCGC 5 cut(s) 87, 162, 569, 688, 710
SspMI CTAG 3 cut(s) 93, 473, 479
StyD4I CCNGG 3 cut(s) 612, 737, 781
TaaI ACNGT 1 cut(s) 563
TaqI TCGA 2 cut(s) 294, 585
TasI AATT 5 cut(s) 127, 173, 258, 349, 498
TatI WGTACW 2 cut(s) 111, 653
TfiI GAWTC 1 cut(s) 580
Tru1I TTAA 3 cut(s) 156, 273, 576
Tru9I TTAA 3 cut(s) 156, 273, 576
TseFI GTSAC 1 cut(s) 557
TseI GCWGC 2 cut(s) 212, 469
Tsp45I GTSAC 1 cut(s) 557
TspDTI ATGAA 2 cut(s) 17, 603
VpaK11BI GGWCC 1 cut(s) 735
XapI RAATTY 1 cut(s) 349
XbaI TCTAGA 1 cut(s) 92
XmnI GAANNNNTTC 1 cut(s) 141
XspI CTAG 3 cut(s) 93, 473, 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.