RLG00000001690
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0009040
Physical Location & Seq
Forward (+)
19161295 .. 19168215
6921 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001690

Sequence Viewer

Length: 966 bp
ATGAGTCTTGCTGGGAATTCCTTAACTAGAAGTATCCCACCTACGCTTTCACTCATGAGCTCTTTGAACTCTCTAAATCTCTCTCGGAATAAACTCTCAGGTGAGGTTCCAGAAAATTTAGAGAAATTGAAGCTAAGTTTCATAGATCTGTCTGAAAATCTGTTTTCTGGAAGAGTACCAACTAATCTTCTGACCACTGGTGGAGACAAAGCCTTTCGTGGAAACAACGAACTCTGCATCGATCAATATTCAAAAACACATTCAAATTCTGGTATGAATACATGTTCTGAAAAGCATAGTCAGAAAAGGGTGCTTCAGAATAAATTGGTTTTGTTCTCTATCATATCATCTGCCTTGGTTGTTGTTTTTGCTGGATTGGTGCTTGTCAGTTATAAGAACTTCAAGCTTTCTGAGGTTGACAGAGAAAATAGTTTGGAAGAGGGGAAGGAAATAGATCCGAAATGGAAACTTGCATCTTTTCACCAACTGGAAATAGATGCAGATGAAATCTGTGATTTTGAAGAAGAAAATTTGCCGGAATTGGATTGGTACCAGAGGTATAGGATTGCTTTGGGAGCAGCTAGGGGGATTTCTTATCTACGCCATGATTGTTCCCCACCTATAATTCATAGAGATATAAAATCAACCAACATTCTGCTTGATGATGATTACGAGGCAAAAGTCGCTGATTTTGGGGTTGCAAAAATTGTACAAAACACTCACAAGGGTTCAGAGTGTAGCTCCTTTGCTGGGACACATGGGTATATTGCTCCCGAGCTGGCATATACTGCTAAACTCACTGAAAAGTGCGATGTATATAGTTTCGGTGTGCTTCCATCCCCGCGGCCTACAATGCGAGAGGTGATCACGATGCTTATTGATGCTGATCCTTCCACCTTCACGTCTCCAAGCAACAATTCTGTCAAAGATGGCAAGGATTCCTCCAGTTTGCTTCTCTTCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

322

Amino Acids

35.62

Weight (kDa)

5.47

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 176 - 278 1.6e-18 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 188 - 278 3.2e-21 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 393
Acc65I GGTACC 1 cut(s) 549
AccB1I GGYRCC 1 cut(s) 549
AccII CGCG 1 cut(s) 844
AciI CCGC 2 cut(s) 842, 844
AclWI GGATC 2 cut(s) 449, 881
AcsI RAATTY 4 cut(s) 16, 115, 265, 529
AcuI CTGAAG 1 cut(s) 299
AfaI GTAC 3 cut(s) 177, 551, 711
AfiI CCNNNNNNNGG 1 cut(s) 750
AflIII ACRYGT 1 cut(s) 281
AgsI TTSAA 6 cut(s) 67, 130, 252, 264, 403, 521
AjiI CACGTC 1 cut(s) 903
AjuI GAANNNNNNNTTGG 2 cut(s) 901, 933
AluBI AGCT 6 cut(s) 60, 133, 406, 581, 741, 778
AluI AGCT 6 cut(s) 60, 133, 406, 581, 741, 778
Alw21I GWGCWC 1 cut(s) 62
Alw26I GTCTC 2 cut(s) 198, 909
AlwI GGATC 2 cut(s) 449, 881
Ama87I CYCGRG 1 cut(s) 773
AoxI GGCC 1 cut(s) 845
ApeKI GCWGC 1 cut(s) 578
ApoI RAATTY 4 cut(s) 16, 115, 265, 529
Asp718I GGTACC 1 cut(s) 549
AsuHPI GGTGA 3 cut(s) 113, 473, 874
AvaI CYCGRG 1 cut(s) 773
BanI GGYRCC 1 cut(s) 549
BanII GRGCYC 1 cut(s) 62
Bbv12I GWGCWC 1 cut(s) 62
BbvI GCAGC 1 cut(s) 590
BccI CCATC 2 cut(s) 844, 923
BciVI GTATCC 1 cut(s) 44
BclI TGATCA 1 cut(s) 864
BcoDI GTCTC 2 cut(s) 198, 909
BfaI CTAG 2 cut(s) 27, 582
BfuI GTATCC 1 cut(s) 44
BglII AGATCT 1 cut(s) 145
BisI GCNGC 2 cut(s) 579, 845
BlsI GCNGC 2 cut(s) 580, 846
BmeT110I CYCGRG 1 cut(s) 773
BmgBI CACGTC 1 cut(s) 903
BmiI GGNNCC 2 cut(s) 108, 551
BmsI GCATC 5 cut(s) 246, 482, 487, 861, 871
BplI GAGNNNNNCTC 2 cut(s) 725, 757
BpmI CTGGAG 1 cut(s) 928
Bsa29I ATCGAT 1 cut(s) 240
BsaBI GATNNNNATC 1 cut(s) 885
BsaJI CCNNGG 2 cut(s) 354, 842
Bsc4I CCNNNNNNNGG 1 cut(s) 750
Bse1I ACTGG 4 cut(s) 202, 492, 945, 961
Bse8I GATNNNNATC 1 cut(s) 885
BseCI ATCGAT 1 cut(s) 240
BseDI CCNNGG 2 cut(s) 354, 842
BseGI GGATG 1 cut(s) 836
BseJI GATNNNNATC 1 cut(s) 885
BseLI CCNNNNNNNGG 1 cut(s) 750
BseMII CTCAG 2 cut(s) 111, 402
BseNI ACTGG 4 cut(s) 202, 492, 945, 961
BseXI GCAGC 1 cut(s) 590
BseYI CCCAGC 2 cut(s) 11, 749
Bsh1236I CGCG 1 cut(s) 844
BshFI GGCC 1 cut(s) 847
BshNI GGYRCC 1 cut(s) 549
BshVI ATCGAT 1 cut(s) 240
BsiHKAI GWGCWC 1 cut(s) 62
BsiHKCI CYCGRG 1 cut(s) 773
BsiSI CCGG 1 cut(s) 536
BslFI GGGAC 1 cut(s) 766
BslI CCNNNNNNNGG 1 cut(s) 750
BsmAI GTCTC 2 cut(s) 198, 909
BsmBI CGTCTC 1 cut(s) 909
BsmFI GGGAC 1 cut(s) 766
BsnI GGCC 1 cut(s) 847
BsoBI CYCGRG 1 cut(s) 773
Bsp1286I GDGCHC 1 cut(s) 62
Bsp1407I TGTACA 1 cut(s) 709
Bsp143I GATC 5 cut(s) 145, 241, 454, 864, 886
BspACI CCGC 2 cut(s) 842, 844
BspANI GGCC 1 cut(s) 847
BspCNI CTCAG 2 cut(s) 110, 403
BspDI ATCGAT 1 cut(s) 240
BspFNI CGCG 1 cut(s) 844
BspHI TCATGA 1 cut(s) 54
BspLI GGNNCC 2 cut(s) 108, 551
BspPI GGATC 2 cut(s) 449, 881
BspT107I GGYRCC 1 cut(s) 549
BsrGI TGTACA 1 cut(s) 709
BsrI ACTGG 4 cut(s) 202, 492, 945, 961
BssECI CCNNGG 2 cut(s) 354, 842
BssMI GATC 5 cut(s) 145, 241, 454, 864, 886
BssT1I CCWWGG 1 cut(s) 354
Bst6I CTCTTC 3 cut(s) 166, 432, 962
BstAPI GCANNNNNTGC 1 cut(s) 788
BstAUI TGTACA 1 cut(s) 709
BstC8I GCNNGC 1 cut(s) 780
BstDEI CTNAG 3 cut(s) 97, 134, 411
BstDSI CCRYGG 1 cut(s) 842
BstF5I GGATG 1 cut(s) 836
BstFNI CGCG 1 cut(s) 844
BstKTI GATC 5 cut(s) 148, 244, 457, 867, 889
BstMAI GTCTC 2 cut(s) 198, 909
BstMBI GATC 5 cut(s) 145, 241, 454, 864, 886
BstMWI GCNNNNNNNGC 4 cut(s) 575, 683, 788, 853
BstNSI RCATGY 1 cut(s) 285
BstUI CGCG 1 cut(s) 844
BstV1I GCAGC 1 cut(s) 590
BstX2I RGATCY 2 cut(s) 145, 454
BstYI RGATCY 2 cut(s) 145, 454
Bsu15I ATCGAT 1 cut(s) 240
BsuI GTATCC 1 cut(s) 44
BsuRI GGCC 1 cut(s) 847
BsuTUI ATCGAT 1 cut(s) 240
BtgI CCRYGG 1 cut(s) 842
BtgZI GCGATG 1 cut(s) 825
BtrI CACGTC 1 cut(s) 903
BtsCI GGATG 1 cut(s) 836
BtsIMutI CAGTG 2 cut(s) 195, 798
Cac8I GCNNGC 1 cut(s) 780
CciI TCATGA 1 cut(s) 54
Cfr42I CCGCGG 1 cut(s) 845
ClaI ATCGAT 1 cut(s) 240
Csp6I GTAC 3 cut(s) 176, 550, 710
CviAII CATG 4 cut(s) 55, 282, 605, 758
CviJI RGCY 8 cut(s) 60, 133, 212, 406, 581, 741, 778, 847
CviKI_1 RGCY 8 cut(s) 60, 133, 212, 406, 581, 741, 778, 847
CviQI GTAC 3 cut(s) 176, 550, 710
DdeI CTNAG 3 cut(s) 97, 134, 411
DpnI GATC 5 cut(s) 147, 243, 456, 866, 888
DpnII GATC 5 cut(s) 145, 241, 454, 864, 886
Eam1104I CTCTTC 3 cut(s) 166, 432, 962
EarI CTCTTC 3 cut(s) 166, 432, 962
Ecl136II GAGCTC 1 cut(s) 60
Eco130I CCWWGG 1 cut(s) 354
Eco24I GRGCYC 1 cut(s) 62
Eco53kI GAGCTC 1 cut(s) 60
Eco57I CTGAAG 1 cut(s) 299
Eco88I CYCGRG 1 cut(s) 773
EcoICRI GAGCTC 1 cut(s) 60
EcoRI GAATTC 1 cut(s) 16
EcoT14I CCWWGG 1 cut(s) 354
EcoT38I GRGCYC 1 cut(s) 62
ErhI CCWWGG 1 cut(s) 354
Esp3I CGTCTC 1 cut(s) 909
FaeI CATG 4 cut(s) 58, 285, 608, 761
FaqI GGGAC 1 cut(s) 766
FatI CATG 4 cut(s) 54, 281, 604, 757
FauI CCCGC 1 cut(s) 849
FbaI TGATCA 1 cut(s) 864
Fnu4HI GCNGC 2 cut(s) 579, 845
FokI GGATG 1 cut(s) 823
FriOI GRGCYC 1 cut(s) 62
Fsp4HI GCNGC 2 cut(s) 579, 845
FspBI CTAG 2 cut(s) 27, 582
GluI GCNGC 2 cut(s) 579, 845
GsaI CCCAGC 2 cut(s) 15, 753
GsuI CTGGAG 1 cut(s) 928
HaeIII GGCC 1 cut(s) 847
HapII CCGG 1 cut(s) 536
Hin1II CATG 4 cut(s) 58, 285, 608, 761
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HindIII AAGCTT 1 cut(s) 404
HinfI GANTC 2 cut(s) 4, 938
HpaII CCGG 1 cut(s) 536
HphI GGTGA 3 cut(s) 113, 473, 874
Hpy166II GTNNAC 1 cut(s) 418
Hpy188I TCNGA 9 cut(s) 87, 154, 192, 289, 303, 318, 412, 459, 733
Hpy188III TCNNGA 5 cut(s) 55, 110, 168, 773, 868
Hpy8I GTNNAC 1 cut(s) 418
HpyAV CCTTC 3 cut(s) 439, 900, 907
HpyCH4IV ACGT 1 cut(s) 902
HpyCH4V TGCA 4 cut(s) 237, 473, 500, 701
HpyF10VI GCNNNNNNNGC 4 cut(s) 575, 683, 788, 853
HpyF3I CTNAG 3 cut(s) 97, 134, 411
HpySE526I ACGT 1 cut(s) 902
Hsp92II CATG 4 cut(s) 58, 285, 608, 761
KpnI GGTACC 1 cut(s) 553
Ksp22I TGATCA 1 cut(s) 864
KspI CCGCGG 1 cut(s) 845
Kzo9I GATC 5 cut(s) 145, 241, 454, 864, 886
LmnI GCTCC 3 cut(s) 575, 746, 775
Lsp1109I GCAGC 1 cut(s) 590
LweI GCATC 5 cut(s) 246, 482, 487, 861, 871
MaeI CTAG 2 cut(s) 27, 582
MaeII ACGT 1 cut(s) 902
MalI GATC 5 cut(s) 147, 243, 456, 866, 888
MboI GATC 5 cut(s) 145, 241, 454, 864, 886
MboII GAAGA 6 cut(s) 179, 183, 449, 533, 536, 949
MflI RGATCY 2 cut(s) 145, 454
MhlI GDGCHC 1 cut(s) 62
MlyI GAGTC 1 cut(s) 13
MnlI CCTC 7 cut(s) 97, 406, 433, 549, 667, 853, 952
MseI TTAA 1 cut(s) 23
MspA1I CMGCKG 1 cut(s) 844
MspI CCGG 1 cut(s) 536
MvnI CGCG 1 cut(s) 844
MwoI GCNNNNNNNGC 4 cut(s) 575, 683, 788, 853
NdeII GATC 5 cut(s) 145, 241, 454, 864, 886
NlaIII CATG 4 cut(s) 58, 285, 608, 761
NlaIV GGNNCC 2 cut(s) 108, 551
NspI RCATGY 1 cut(s) 285
PagI TCATGA 1 cut(s) 54
PciI ACATGT 1 cut(s) 281
PfeI GAWTC 1 cut(s) 938
PkrI GCNGC 2 cut(s) 580, 846
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
PscI ACATGT 1 cut(s) 281
PsiI TTATAA 1 cut(s) 393
Psp124BI GAGCTC 1 cut(s) 62
PspFI CCCAGC 2 cut(s) 11, 749
PspN4I GGNNCC 2 cut(s) 108, 551
PsuI RGATCY 2 cut(s) 145, 454
RsaI GTAC 3 cut(s) 177, 551, 711
RsaNI GTAC 3 cut(s) 176, 550, 710
SacI GAGCTC 1 cut(s) 62
SacII CCGCGG 1 cut(s) 845
SaqAI TTAA 1 cut(s) 23
SatI GCNGC 2 cut(s) 579, 845
Sau3AI GATC 5 cut(s) 145, 241, 454, 864, 886
SchI GAGTC 1 cut(s) 13
SduI GDGCHC 1 cut(s) 62
SfaNI GCATC 5 cut(s) 246, 482, 487, 861, 871
Sfr303I CCGCGG 1 cut(s) 845
SgrBI CCGCGG 1 cut(s) 845
SsiI CCGC 2 cut(s) 842, 844
SspI AATATT 1 cut(s) 248
SspMI CTAG 2 cut(s) 27, 582
SstI GAGCTC 1 cut(s) 62
StyI CCWWGG 1 cut(s) 354
TaiI ACGT 1 cut(s) 905
TaqI TCGA 1 cut(s) 240
TatI WGTACW 1 cut(s) 709
TauI GCSGC 1 cut(s) 847
TfiI GAWTC 1 cut(s) 938
Tru1I TTAA 1 cut(s) 23
Tru9I TTAA 1 cut(s) 23
TscAI CASTG 2 cut(s) 202, 805
TseI GCWGC 1 cut(s) 578
TspDTI ATGAA 4 cut(s) 130, 290, 519, 617
TspRI CASTG 2 cut(s) 202, 805
XapI RAATTY 4 cut(s) 16, 115, 265, 529
XceI RCATGY 1 cut(s) 285
XspI CTAG 2 cut(s) 27, 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.