RLG00000001702
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0009040
Physical Location & Seq
Forward (+)
19297238 .. 19314775
17538 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001702

Sequence Viewer

Length: 1059 bp
ATGAGTCTTGCGGGGAATTCCTTAACTAGAAGTATCCCACCTACGCTTTCACTCATGAGCTCTTTGAACGCTCTAAATCTCTCTCGGAATAAACTCTCAGGTGAGGTTCCAGAAAATTTAGAGAAATTGAAGCTAAGTTTCATAGTTCTGTCTGAAAATCTGTTATCTGGAAGAGTACCATCTGATCTTCTGACCACTGGTGGAGACAAAGCCTTTCGTGGAAACAACGAACTCTGCATCGATCAATATTCAAAAACACATTCAAATTCTGGATTGGTGCTTGTCAATTATAAGAACTTCAAGGTTGACAGAGAAAACAGTTTGGAAGAGGGGAAGGAAATAGATCCAAAATGGAAACTTGCATCTTTTCACCAACTGGAAATAGATGCAGATGAAATATGTGATTTTGAAGAAGAAAATTTGGTTGGTAGTGGCAGTACAAGGAAAAGGTATAGGATTGCTTTGGGACAAGCTAGGGGGATTTCTTATCTACGCCATGATTGTTCCCCACCTATAATTCATAGAGACATAAAATCAACCAACATTCTGCTTGATGATGAGTACGAGGCAAAAGTTGCTGATTTTGGGGTTGCAAAAATTGTACAAAACACTCACAAGGGTTCAGAGTGCAGCTCCTTTGCTGGGACACATGGGTATATTGCTCTTGAGCTGGCATATACTGCTAAAGTCACTGAAAAGTGTGATGTATATAGTTTCGATGTGGTTCTACTTGAGCTAGTTACTGGTAGAAGACCAATTGAAGATGACTACGGAGAAGGAAAAGACATTGTATACTGGGTTTCTGCCAATCTGAACGACCATGAAAATGTTCAAAAGGTTCTTGATGATAAAGTGGCAGATGAAAATATCCAGGATGACATGATCAAGGCTTTGAAGGTAGCTATTCTTTGTACTACCAAGCTTCCATCCCTGCGCCCTACAATGCGAGAGGTGATCAAGATGCTTATTGATGCTGATCCTTCCACCTTCACGTCTCCAAGCAACAATTCTGTCAAAGAAGGCAATGATTCCTCCAGTCTGCTTCTCTCCCAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

353

Amino Acids

39.05

Weight (kDa)

5.18

Isoelectric Point (pI)

33.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 147 - 322 2.5e-21 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 152 - 320 1.5e-24 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 291
AccI GTMKAC 1 cut(s) 792
AciI CCGC 1 cut(s) 11
AclWI GGATC 2 cut(s) 338, 971
AcsI RAATTY 4 cut(s) 16, 115, 265, 418
AfaI GTAC 5 cut(s) 177, 439, 563, 603, 913
AfiI CCNNNNNNNGG 1 cut(s) 642
AgsI TTSAA 9 cut(s) 67, 130, 252, 264, 301, 410, 761, 833, 895
AjiI CACGTC 1 cut(s) 993
AjnI CCWGG 1 cut(s) 870
AjuI GAANNNNNNNTTGG 4 cut(s) 408, 440, 991, 1023
AluBI AGCT 8 cut(s) 60, 133, 473, 633, 670, 736, 902, 922
AluI AGCT 8 cut(s) 60, 133, 473, 633, 670, 736, 902, 922
Alw21I GWGCWC 1 cut(s) 62
Alw26I GTCTC 3 cut(s) 198, 519, 999
AlwI GGATC 2 cut(s) 338, 971
ApeKI GCWGC 1 cut(s) 630
ApoI RAATTY 4 cut(s) 16, 115, 265, 418
Asp700I GAANNNNTTC 1 cut(s) 828
AspLEI GCGC 1 cut(s) 936
AsuHPI GGTGA 3 cut(s) 113, 362, 964
BanII GRGCYC 1 cut(s) 62
BbsI GAAGAC 1 cut(s) 757
Bbv12I GWGCWC 1 cut(s) 62
BbvI GCAGC 1 cut(s) 642
BccI CCATC 2 cut(s) 187, 934
BciT130I CCWGG 1 cut(s) 872
BciVI GTATCC 1 cut(s) 44
BclI TGATCA 2 cut(s) 882, 954
BcoDI GTCTC 3 cut(s) 198, 519, 999
BfaI CTAG 3 cut(s) 27, 474, 737
BfuI GTATCC 1 cut(s) 44
BisI GCNGC 1 cut(s) 631
BlsI GCNGC 1 cut(s) 632
Bme1390I CCNGG 1 cut(s) 872
BmgBI CACGTC 1 cut(s) 993
BmiI GGNNCC 1 cut(s) 108
BmrFI CCNGG 1 cut(s) 872
BmrI ACTGGG 1 cut(s) 805
BmsI GCATC 5 cut(s) 246, 371, 376, 951, 961
BmuI ACTGGG 1 cut(s) 805
BpiI GAAGAC 1 cut(s) 757
BplI GAGNNNNNCTC 2 cut(s) 617, 649
BpmI CTGGAG 1 cut(s) 1018
BpuEI CTTGAG 2 cut(s) 686, 752
Bsa29I ATCGAT 1 cut(s) 240
BsaBI GATNNNNATC 1 cut(s) 975
Bsc4I CCNNNNNNNGG 1 cut(s) 642
Bse1I ACTGG 5 cut(s) 202, 381, 748, 800, 1035
Bse3DI GCAATG 1 cut(s) 1030
Bse8I GATNNNNATC 1 cut(s) 975
BseBI CCWGG 1 cut(s) 872
BseCI ATCGAT 1 cut(s) 240
BseGI GGATG 2 cut(s) 880, 926
BseJI GATNNNNATC 1 cut(s) 975
BseLI CCNNNNNNNGG 1 cut(s) 642
BseMI GCAATG 1 cut(s) 1030
BseMII CTCAG 1 cut(s) 111
BseNI ACTGG 5 cut(s) 202, 381, 748, 800, 1035
BseXI GCAGC 1 cut(s) 642
BseYI CCCAGC 1 cut(s) 641
BsgI GTGCAG 1 cut(s) 649
BshVI ATCGAT 1 cut(s) 240
BsiHKAI GWGCWC 1 cut(s) 62
BslFI GGGAC 2 cut(s) 480, 658
BslI CCNNNNNNNGG 1 cut(s) 642
BsmAI GTCTC 3 cut(s) 198, 519, 999
BsmBI CGTCTC 1 cut(s) 999
BsmFI GGGAC 2 cut(s) 480, 658
Bsp1286I GDGCHC 1 cut(s) 62
Bsp1407I TGTACA 1 cut(s) 601
Bsp143I GATC 6 cut(s) 184, 241, 343, 882, 954, 976
BspACI CCGC 1 cut(s) 11
BspCNI CTCAG 1 cut(s) 110
BspDI ATCGAT 1 cut(s) 240
BspHI TCATGA 1 cut(s) 54
BspLI GGNNCC 1 cut(s) 108
BspPI GGATC 2 cut(s) 338, 971
BsrDI GCAATG 1 cut(s) 1030
BsrGI TGTACA 1 cut(s) 601
BsrI ACTGG 5 cut(s) 202, 381, 748, 800, 1035
BssMI GATC 6 cut(s) 184, 241, 343, 882, 954, 976
BssNAI GTATAC 1 cut(s) 793
Bst1107I GTATAC 1 cut(s) 793
Bst2UI CCWGG 1 cut(s) 872
Bst4CI ACNGT 1 cut(s) 320
Bst6I CTCTTC 2 cut(s) 166, 321
BstAPI GCANNNNNTGC 2 cut(s) 575, 680
BstAUI TGTACA 1 cut(s) 601
BstC8I GCNNGC 1 cut(s) 672
BstDEI CTNAG 2 cut(s) 97, 134
BstF5I GGATG 2 cut(s) 880, 926
BstHHI GCGC 1 cut(s) 936
BstKTI GATC 6 cut(s) 187, 244, 346, 885, 957, 979
BstMAI GTCTC 3 cut(s) 198, 519, 999
BstMBI GATC 6 cut(s) 184, 241, 343, 882, 954, 976
BstMWI GCNNNNNNNGC 2 cut(s) 575, 680
BstNI CCWGG 1 cut(s) 872
BstSCI CCNGG 1 cut(s) 870
BstV1I GCAGC 1 cut(s) 642
BstV2I GAAGAC 1 cut(s) 757
BstX2I RGATCY 1 cut(s) 343
BstYI RGATCY 1 cut(s) 343
BstZ17I GTATAC 1 cut(s) 793
Bsu15I ATCGAT 1 cut(s) 240
BsuI GTATCC 1 cut(s) 44
BsuTUI ATCGAT 1 cut(s) 240
BtrI CACGTC 1 cut(s) 993
BtsCI GGATG 2 cut(s) 880, 926
BtsIMutI CAGTG 2 cut(s) 195, 690
Cac8I GCNNGC 1 cut(s) 672
CciI TCATGA 1 cut(s) 54
CfoI GCGC 1 cut(s) 936
ClaI ATCGAT 1 cut(s) 240
Csp6I GTAC 5 cut(s) 176, 438, 562, 602, 912
CviAII CATG 5 cut(s) 55, 497, 650, 821, 880
CviQI GTAC 5 cut(s) 176, 438, 562, 602, 912
DdeI CTNAG 2 cut(s) 97, 134
DpnI GATC 6 cut(s) 186, 243, 345, 884, 956, 978
DpnII GATC 6 cut(s) 184, 241, 343, 882, 954, 976
Eam1104I CTCTTC 2 cut(s) 166, 321
EarI CTCTTC 2 cut(s) 166, 321
Ecl136II GAGCTC 1 cut(s) 60
Eco24I GRGCYC 1 cut(s) 62
Eco53kI GAGCTC 1 cut(s) 60
EcoICRI GAGCTC 1 cut(s) 60
EcoRI GAATTC 1 cut(s) 16
EcoRII CCWGG 1 cut(s) 870
EcoT38I GRGCYC 1 cut(s) 62
Esp3I CGTCTC 1 cut(s) 999
FaeI CATG 5 cut(s) 58, 500, 653, 824, 883
FaqI GGGAC 2 cut(s) 480, 658
FatI CATG 5 cut(s) 54, 496, 649, 820, 879
FauI CCCGC 1 cut(s) 4
FbaI TGATCA 2 cut(s) 882, 954
FblI GTMKAC 1 cut(s) 792
Fnu4HI GCNGC 1 cut(s) 631
FokI GGATG 2 cut(s) 887, 913
FriOI GRGCYC 1 cut(s) 62
Fsp4HI GCNGC 1 cut(s) 631
FspBI CTAG 3 cut(s) 27, 474, 737
GlaI GCGC 1 cut(s) 935
GluI GCNGC 1 cut(s) 631
GsaI CCCAGC 1 cut(s) 645
GsuI CTGGAG 1 cut(s) 1018
HhaI GCGC 1 cut(s) 936
Hin1II CATG 5 cut(s) 58, 500, 653, 824, 883
Hin6I GCGC 1 cut(s) 934
HinP1I GCGC 1 cut(s) 934
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HindIII AAGCTT 1 cut(s) 920
HinfI GANTC 2 cut(s) 4, 1028
HphI GGTGA 3 cut(s) 113, 362, 964
Hpy166II GTNNAC 2 cut(s) 307, 793
Hpy188I TCNGA 6 cut(s) 87, 154, 184, 192, 625, 813
Hpy188III TCNNGA 7 cut(s) 55, 110, 168, 270, 665, 842, 958
Hpy8I GTNNAC 2 cut(s) 307, 793
HpyAV CCTTC 6 cut(s) 328, 770, 889, 990, 997, 1013
HpyCH4III ACNGT 1 cut(s) 320
HpyCH4IV ACGT 1 cut(s) 992
HpyCH4V TGCA 5 cut(s) 237, 362, 389, 593, 630
HpyF10VI GCNNNNNNNGC 2 cut(s) 575, 680
HpyF3I CTNAG 2 cut(s) 97, 134
HpySE526I ACGT 1 cut(s) 992
Hsp92II CATG 5 cut(s) 58, 500, 653, 824, 883
HspAI GCGC 1 cut(s) 934
Ksp22I TGATCA 2 cut(s) 882, 954
Kzo9I GATC 6 cut(s) 184, 241, 343, 882, 954, 976
LmnI GCTCC 1 cut(s) 638
Lsp1109I GCAGC 1 cut(s) 642
LweI GCATC 5 cut(s) 246, 371, 376, 951, 961
MaeI CTAG 3 cut(s) 27, 474, 737
MaeII ACGT 1 cut(s) 992
MaeIII GTNAC 2 cut(s) 688, 739
MalI GATC 6 cut(s) 186, 243, 345, 884, 956, 978
MboI GATC 6 cut(s) 184, 241, 343, 882, 954, 976
MboII GAAGA 7 cut(s) 179, 183, 338, 422, 425, 762, 773
MfeI CAATTG 1 cut(s) 756
MflI RGATCY 1 cut(s) 343
MhlI GDGCHC 1 cut(s) 62
MlyI GAGTC 1 cut(s) 13
MnlI CCTC 5 cut(s) 97, 322, 559, 943, 1042
MroXI GAANNNNTTC 1 cut(s) 828
MseI TTAA 1 cut(s) 23
MslI CAYNNNNRTG 1 cut(s) 825
MspR9I CCNGG 1 cut(s) 872
MunI CAATTG 1 cut(s) 756
MvaI CCWGG 1 cut(s) 872
MwoI GCNNNNNNNGC 2 cut(s) 575, 680
NdeII GATC 6 cut(s) 184, 241, 343, 882, 954, 976
NlaIII CATG 5 cut(s) 58, 500, 653, 824, 883
NlaIV GGNNCC 1 cut(s) 108
NmuCI GTSAC 1 cut(s) 688
PagI TCATGA 1 cut(s) 54
PdmI GAANNNNTTC 1 cut(s) 828
PfeI GAWTC 1 cut(s) 1028
PfoI TCCNGGA 1 cut(s) 870
PkrI GCNGC 1 cut(s) 632
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
PsiI TTATAA 1 cut(s) 291
Psp124BI GAGCTC 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 870
PspFI CCCAGC 1 cut(s) 641
PspGI CCWGG 1 cut(s) 870
PspN4I GGNNCC 1 cut(s) 108
PsuI RGATCY 1 cut(s) 343
RsaI GTAC 5 cut(s) 177, 439, 563, 603, 913
RsaNI GTAC 5 cut(s) 176, 438, 562, 602, 912
RseI CAYNNNNRTG 1 cut(s) 825
SacI GAGCTC 1 cut(s) 62
SaqAI TTAA 1 cut(s) 23
SatI GCNGC 1 cut(s) 631
Sau3AI GATC 6 cut(s) 184, 241, 343, 882, 954, 976
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 1 cut(s) 872
SduI GDGCHC 1 cut(s) 62
SfaNI GCATC 5 cut(s) 246, 371, 376, 951, 961
SmiMI CAYNNNNRTG 1 cut(s) 825
SmlI CTYRAG 2 cut(s) 665, 731
SmoI CTYRAG 2 cut(s) 665, 731
SsiI CCGC 1 cut(s) 11
SspI AATATT 1 cut(s) 248
SspMI CTAG 3 cut(s) 27, 474, 737
SstI GAGCTC 1 cut(s) 62
StyD4I CCNGG 1 cut(s) 870
TaaI ACNGT 1 cut(s) 320
TaiI ACGT 1 cut(s) 995
TaqI TCGA 2 cut(s) 240, 717
TatI WGTACW 3 cut(s) 437, 601, 911
TfiI GAWTC 1 cut(s) 1028
Tru1I TTAA 1 cut(s) 23
Tru9I TTAA 1 cut(s) 23
TscAI CASTG 2 cut(s) 202, 697
TseFI GTSAC 1 cut(s) 688
TseI GCWGC 1 cut(s) 630
Tsp45I GTSAC 1 cut(s) 688
TspDTI ATGAA 5 cut(s) 130, 408, 509, 837, 876
TspGWI ACGGA 1 cut(s) 786
TspRI CASTG 2 cut(s) 202, 697
XapI RAATTY 4 cut(s) 16, 115, 265, 418
XmiI GTMKAC 1 cut(s) 792
XmnI GAANNNNTTC 1 cut(s) 828
XspI CTAG 3 cut(s) 27, 474, 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.