RLG00000001687
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0009040
Physical Location & Seq
Forward (+)
19142917 .. 19146666
3750 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001687

Sequence Viewer

Length: 2955 bp
ATGGCCAAAATACCAGTTCCCTCCTTGCACTTGCTACCTGCAGTCTTTACCACATTGTTCATTTCTTTGCTTTTCCCGCCATGTATGCCTCTAACGGTCGAAACTGAAGCCCTCCTTGAGTTCAAAAGGCAGCTCAAGGACCCTTTGAGTTTTCTTGATTCGTGGAAAGAGTCGGAGTCTTCTTCCCCCTGTGAGTTCTCCGGGATTACTTGCAATTCGGGGAAAGTCACTGAAATATCTCTTCACAACAAGTCACTCTCAGGTGAGATTTCGCCCTCCATTGGCATCCTTGAGAGCCTCACCACGCTTGTACTGTCTGCCAACCTCATTACAGGAGAGGTTCCTGCTCAACTGACTCATTGTGCCATTCTTAAAACATTGAATCTGTCTGAGAATCAATTGGTTGGAAAAATCCCGGATCTTTCCACCCTTAGAAAATTGGAGAGACTTGATCTTTCTGCCAACAGGTTATCTTCAGAGTTCCCGTCTTGGGTGGGAAATCTCACAGGTTTACAATATCTGGGCCTTGGTCAAAATCTGTTTGATGCAAGCAAAGTTCCTGGGAGTCTTGGAAACTTGAAGAACTTGACATGGCTGTATCTGACAAATTGCAGCTTGATGGGAGAGATTCCGGAATCTATTTACGAAATGAAGGCATTGGGAACATTTGATATGTCAAGAAACAATCTTTCTGGGAAACTCTCCAAGTCGATTTCCAAGCTACAAAACCTATTTAAGATTGAGCTGTTTGCAAACGGTTTGGGTGGAGAAATCCCACCCGAGGTTGCTAATCTCACCCTTCTGAGGGAATTTGATATTTCAACAAACAAATTTTCTGGGAAATTGCCTTCTGAGTTTGGAAATCTCAAGAATTTAGTGGTTTTTCAGTTGTATGAAAACAATTTTTCGGGGGAATTCCCTGCTGGGTTTGGAGATATGCAGCATCTTACTGCTTTGTCAATATATGGAAACAGATTTTCTGGGGAGTTTCCTGCAAATCTTGGGAAATTCTCACTTTTGGAGAGCATTGACATATCTGAGAATCAGTTTTCTGGTGCTTTCCCAAGGTTTCTTTGTGAAAAGAGGAAGCTACAATTCTTGCTCGCTTTGGAGAACAATTTTTATGGGGAGTTTCCAGATTCTTATGCTGACTGTAAATCTCTAGAGAGGTTTAGAGTAAGTAAGAACCATTTGTATGGGGAGATTCCAGCTGAGTTTTGGTCACTTCCTAAAGCGACAATCATTGAGTTTAGTGACAATAACTTTCGTGGAGTGATTTCCCCCAGCATTGGGTTTTCTACCAGCTTGAATCAGTTGATGTTGCAGAACAACAATTTCTCAGGTTATCTTCCACCGGAAATTGGGAAGCTAACAAAGTTGGACAAGCTCTATTTGAATCATAATAACTTTTCAGGTGAAATACCTTCTGAAATTGGTGCCTTGAAGCACTTATCATCTTTCCATGTTGAAGAAAATTCTTTGTCAGGCTTAATACCTTCAGAACTGGGAAGCTGTGTTAGGTTGGCGGACATGAGTCTTGCTGGGAATTCCTTAACTGGAAGTATCCCACCTACGCTTTCACTCATGAGCTCTTTGAACTCTCTAGATCTCTCTCGGAATAAACTCTCAGGTGAGGTTCCAGAAAATTTAGAGAAATTGAAGCTAAGTTTCATAGATTTGTCTGAAAATCTGTTATCTGGAAGAGTACCATCTGATCTTCTGACCACTGGTGGAGACAAAGCCTTTCGGGGAAACAACGATCTCTGCATCGATCAATATTCAAAAACACATTCAAATTCTGGTATGAATACATGTTCTGAAAAGCATAGTCAGAAAAGGGTGCTTCAGAATAAATTGGTTTTGTTCTCTATCATAGCATCTGCCTTGGTTGTTGTTTTAGCTGGATTGGTGCTTGTCAGTTATAAGAACTTTAAGCTTTCTGAGGTTGACAGAGAAAACAGTTTGAAAGAGGGGAAGGAAATAGATCCGAAATGGAAACTTGCATCTTTTCACCAACTGGAAATAGATGCAGATGAAATCTGTGATTTAGAAGAAGAAAATTTGGTTGGTAGTGGCAGCACCGGGAAAGTTTACCGCCTTGATCTGAAGAAGAATGGTGGTACAGTAGCTGTAAAACAACTATGGAAAGACGACGGCTTGAAGCTTATGACTGCAGAGATGGATATTTTGGGAAAGATCAGGCATAGAAATATATTGAAGCTTTATGCTTGTTTAGTGAAGGGAGGATCTAATCTTCTGGTGTTTGAGTATATGGAAAATGGAAATTTGTTTCAAGCGCTTCACAGACAGCTTAAAGGTGGGCAGCCGGAATTGGATTGGTACCAGAGGTATAGGATTGCTTTGGGAGCGGCTAGGGGGATTTCTTATCTACACCATGATTGTTCCCCACCTATAATTCATAGAGATATAAAATCAACCAACATTCTGCTTGATGATGATTACGAGGCAAAAGTTGCTGATTTTGGGGTTGCAAAAATTGTACAGAACACTCCCAAGGGTTCAGAGTGCAGCTCCTTTGCTGGGACACATGGGTATATTGCTCCTGAGCTGGCATATACTGCTAGAGTCACTGAAAAGTGTGATGTATATAGTTTCGGTGTGGTTCTACTTGAGCTAGTTACTGGTAGAAGACCAATTGAAGATGACTACGGGGAAGGAAAAGACATTGTATACTGGGTTTCTGCCAATCTGAACGAACGTGAAAACGTTCTGAAGGTTCTTGATGATAAAGTGGCAGATGAAAATATCCAGGATGACATGATCAAGGTATTGAAGGTAGCTATTCTTTGTACTACCAAGCTTCCATCCCTACGCCCTACAATGCGAGAGGTGATCAAGATGCTTATTGATGCTGATCCTGGCACTTTCACGTCTCCAAGCAACAATTCTGTCAAAGATGGCAAGGATTCCTCCAGTCTGCTTCTCTCCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

985

Amino Acids

108.74

Weight (kDa)

5.59

Isoelectric Point (pI)

33.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 34 - 72 4.2e-11 Leucine rich repeat N-terminal domain
LRR_8 PF13855 145 - 206 6.4e-09 Leucine rich repeat
LRR_14 PF23598 164 - 249 2.5e-06 Leucine-rich repeat region
LRR_14 PF23598 257 - 404 6.3e-10 Leucine-rich repeat region
LRR_14 PF23598 436 - 544 1.8e-06 Leucine-rich repeat region
Pkinase PF00069 684 - 953 3.8e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 686 - 955 1e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1923
Acc36I ACCTGC 1 cut(s) 46
Acc65I GGTACC 1 cut(s) 2340
AccB1I GGYRCC 2 cut(s) 1436, 2340
AccBSI CCGCTC 1 cut(s) 2369
AccI GTMKAC 1 cut(s) 2691
AccIII TCCGGA 1 cut(s) 631
AciI CCGC 4 cut(s) 77, 1526, 2095, 2369
AclI AACGTT 1 cut(s) 2727
AclWI GGATC 4 cut(s) 426, 1979, 2254, 2870
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 6 cut(s) 126, 459, 1482, 1829, 2126, 2753
AfaI GTAC 6 cut(s) 312, 1707, 2122, 2342, 2502, 2812
AfeI AGCGCT 1 cut(s) 2298
AfiI CCNNNNNNNGG 9 cut(s) 281, 490, 781, 804, 805, 1289, 1290, 1361, 2541
AflIII ACRYGT 1 cut(s) 1811
AjiI CACGTC 1 cut(s) 2892
AjnI CCWGG 3 cut(s) 559, 2769, 2878
AjuI GAANNNNNNNTTGG 4 cut(s) 2049, 2081, 2890, 2922
Alw21I GWGCWC 1 cut(s) 1592
Alw26I GTCTC 3 cut(s) 439, 1728, 2898
AlwI GGATC 4 cut(s) 426, 1979, 2254, 2870
AlwNI CAGNNNCTG 1 cut(s) 2129
Ama87I CYCGRG 1 cut(s) 779
Aor13HI TCCGGA 1 cut(s) 631
Aor51HI AGCGCT 1 cut(s) 2298
AoxI GGCC 2 cut(s) 3, 523
ApeKI GCWGC 6 cut(s) 130, 612, 940, 2076, 2323, 2529
Asp700I GAANNNNTTC 1 cut(s) 2727
Asp718I GGTACC 1 cut(s) 2340
AspLEI GCGC 1 cut(s) 2299
AspS9I GGNCC 2 cut(s) 139, 523
AsuC2I CCSGG 3 cut(s) 202, 416, 2083
AsuHPI GGTGA 7 cut(s) 275, 292, 787, 1427, 1643, 2003, 2863
AvaI CYCGRG 1 cut(s) 779
AvaII GGWCC 1 cut(s) 139
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 1436, 2340
BanII GRGCYC 1 cut(s) 1592
BbsI GAAGAC 2 cut(s) 171, 2656
Bbv12I GWGCWC 1 cut(s) 1592
BbvI GCAGC 6 cut(s) 142, 624, 952, 2088, 2335, 2541
BccI CCATC 5 cut(s) 613, 1717, 2173, 2833, 2912
BceAI ACGGC 1 cut(s) 2170
BciT130I CCWGG 3 cut(s) 561, 2771, 2880
BciVI GTATCC 1 cut(s) 1574
BclI TGATCA 2 cut(s) 2781, 2853
BcnI CCSGG 3 cut(s) 202, 416, 2083
BcoDI GTCTC 3 cut(s) 439, 1728, 2898
BfaI CTAG 5 cut(s) 1163, 1604, 2373, 2583, 2636
BfmI CTRYAG 2 cut(s) 39, 2172
BfoI RGCGCY 1 cut(s) 2300
BfuAI ACCTGC 1 cut(s) 46
BfuI GTATCC 1 cut(s) 1574
BglII AGATCT 1 cut(s) 1606
BisI GCNGC 7 cut(s) 131, 613, 941, 2077, 2324, 2370, 2530
BlsI GCNGC 7 cut(s) 132, 614, 942, 2078, 2325, 2371, 2531
Bme1390I CCNGG 6 cut(s) 202, 416, 561, 2083, 2771, 2880
Bme18I GGWCC 1 cut(s) 139
BmeT110I CYCGRG 1 cut(s) 779
BmgBI CACGTC 1 cut(s) 2892
BmgT120I GGNCC 2 cut(s) 139, 523
BmiI GGNNCC 5 cut(s) 141, 342, 1438, 1638, 2342
BmrFI CCNGG 6 cut(s) 202, 416, 561, 2083, 2771, 2880
BmrI ACTGGG 3 cut(s) 1514, 2704, 2944
BmsI GCATC 9 cut(s) 294, 535, 952, 1776, 1886, 2012, 2017, 2850, 2860
BmuI ACTGGG 3 cut(s) 1514, 2704, 2944
BoxI GACNNNNGTC 1 cut(s) 1533
BpiI GAAGAC 2 cut(s) 171, 2656
BplI GAGNNNNNCTC 2 cut(s) 2516, 2548
BpmI CTGGAG 1 cut(s) 2917
Bpu10I CCTNAGC 1 cut(s) 2565
BpuEI CTTGAG 5 cut(s) 119, 137, 311, 851, 2651
BpuMI CCSGG 3 cut(s) 202, 416, 2083
Bsa29I ATCGAT 1 cut(s) 1770
BsaBI GATNNNNATC 1 cut(s) 2874
BsaJI CCNNGG 6 cut(s) 526, 560, 780, 1064, 1884, 2514
BsaWI WCCGGW 2 cut(s) 631, 1354
Bsc4I CCNNNNNNNGG 9 cut(s) 281, 490, 781, 804, 805, 1289, 1290, 1361, 2541
Bse1I ACTGG 9 cut(s) 14, 1509, 1561, 1732, 2022, 2647, 2699, 2934, 2950
Bse8I GATNNNNATC 1 cut(s) 2874
BseAI TCCGGA 1 cut(s) 631
BseBI CCWGG 3 cut(s) 561, 2771, 2880
BseCI ATCGAT 1 cut(s) 1770
BseDI CCNNGG 6 cut(s) 526, 560, 780, 1064, 1884, 2514
BseGI GGATG 3 cut(s) 285, 2779, 2825
BseJI GATNNNNATC 1 cut(s) 2874
BseLI CCNNNNNNNGG 9 cut(s) 281, 490, 781, 804, 805, 1289, 1290, 1361, 2541
BseNI ACTGG 9 cut(s) 14, 1509, 1561, 1732, 2022, 2647, 2699, 2934, 2950
BseXI GCAGC 6 cut(s) 142, 624, 952, 2088, 2335, 2541
BseYI CCCAGC 4 cut(s) 923, 1283, 1541, 2540
BsgI GTGCAG 1 cut(s) 2548
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 2 cut(s) 5, 525
BshNI GGYRCC 2 cut(s) 1436, 2340
BshVI ATCGAT 1 cut(s) 1770
BsiEI CGRYCG 1 cut(s) 99
BsiHKAI GWGCWC 1 cut(s) 1592
BsiHKCI CYCGRG 1 cut(s) 779
BsiSI CCGG 6 cut(s) 201, 416, 632, 1355, 2082, 2327
BslFI GGGAC 1 cut(s) 2557
BslI CCNNNNNNNGG 9 cut(s) 281, 490, 781, 804, 805, 1289, 1290, 1361, 2541
BsmAI GTCTC 3 cut(s) 439, 1728, 2898
BsmBI CGTCTC 1 cut(s) 2898
BsmFI GGGAC 1 cut(s) 2557
BsnI GGCC 2 cut(s) 5, 525
BsoBI CYCGRG 1 cut(s) 779
Bsp1286I GDGCHC 1 cut(s) 1592
Bsp13I TCCGGA 1 cut(s) 631
Bsp1407I TGTACA 1 cut(s) 2500
BspACI CCGC 4 cut(s) 77, 1526, 2095, 2369
BspANI GGCC 2 cut(s) 5, 525
BspDI ATCGAT 1 cut(s) 1770
BspEI TCCGGA 1 cut(s) 631
BspHI TCATGA 1 cut(s) 1584
BspLI GGNNCC 5 cut(s) 141, 342, 1438, 1638, 2342
BspMAI CTGCAG 2 cut(s) 43, 2176
BspMI ACCTGC 1 cut(s) 46
BspPI GGATC 4 cut(s) 426, 1979, 2254, 2870
BspT107I GGYRCC 2 cut(s) 1436, 2340
BsrBI CCGCTC 1 cut(s) 2369
BsrGI TGTACA 1 cut(s) 2500
BsrI ACTGG 9 cut(s) 14, 1509, 1561, 1732, 2022, 2647, 2699, 2934, 2950
BssECI CCNNGG 6 cut(s) 526, 560, 780, 1064, 1884, 2514
BssNAI GTATAC 1 cut(s) 2692
BssT1I CCWWGG 4 cut(s) 526, 1064, 1884, 2514
Bst1107I GTATAC 1 cut(s) 2692
Bst2UI CCWGG 3 cut(s) 561, 2771, 2880
Bst4CI ACNGT 6 cut(s) 97, 315, 758, 1154, 1961, 2125
Bst6I CTCTTC 2 cut(s) 246, 1696
BstAPI GCANNNNNTGC 2 cut(s) 2474, 2579
BstAUI TGTACA 1 cut(s) 2500
BstC8I GCNNGC 3 cut(s) 550, 1104, 2571
BstF5I GGATG 3 cut(s) 285, 2779, 2825
BstH2I RGCGCY 1 cut(s) 2300
BstHHI GCGC 1 cut(s) 2299
BstMAI GTCTC 3 cut(s) 439, 1728, 2898
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 5 cut(s) 76, 85, 2366, 2474, 2579
BstNI CCWGG 3 cut(s) 561, 2771, 2880
BstNSI RCATGY 1 cut(s) 1815
BstPAI GACNNNNGTC 1 cut(s) 1533
BstSCI CCNGG 6 cut(s) 200, 414, 559, 2081, 2769, 2878
BstSFI CTRYAG 2 cut(s) 39, 2172
BstV1I GCAGC 6 cut(s) 142, 624, 952, 2088, 2335, 2541
BstV2I GAAGAC 2 cut(s) 171, 2656
BstX2I RGATCY 4 cut(s) 418, 1606, 1984, 2246
BstXI CCANNNNNNTGG 1 cut(s) 1196
BstYI RGATCY 4 cut(s) 418, 1606, 1984, 2246
BstZ17I GTATAC 1 cut(s) 2692
Bsu15I ATCGAT 1 cut(s) 1770
BsuI GTATCC 1 cut(s) 1574
BsuRI GGCC 2 cut(s) 5, 525
BsuTUI ATCGAT 1 cut(s) 1770
BtrI CACGTC 1 cut(s) 2892
BtsCI GGATG 3 cut(s) 285, 2779, 2825
BtsIMutI CAGTG 3 cut(s) 228, 1725, 2589
BveI ACCTGC 1 cut(s) 46
Cac8I GCNNGC 3 cut(s) 550, 1104, 2571
CaiI CAGNNNCTG 1 cut(s) 2129
CciI TCATGA 1 cut(s) 1584
CfoI GCGC 1 cut(s) 2299
Cfr13I GGNCC 2 cut(s) 139, 523
ClaI ATCGAT 1 cut(s) 1770
Csp6I GTAC 6 cut(s) 311, 1706, 2121, 2341, 2501, 2811
CviAII CATG 9 cut(s) 81, 591, 1463, 1531, 1585, 1812, 2396, 2549, 2779
CviQI GTAC 6 cut(s) 311, 1706, 2121, 2341, 2501, 2811
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 246, 1696
EarI CTCTTC 2 cut(s) 246, 1696
EciI GGCGGA 1 cut(s) 1541
Ecl136II GAGCTC 1 cut(s) 1590
Eco130I CCWWGG 4 cut(s) 526, 1064, 1884, 2514
Eco24I GRGCYC 1 cut(s) 1592
Eco47I GGWCC 1 cut(s) 139
Eco47III AGCGCT 1 cut(s) 2298
Eco53kI GAGCTC 1 cut(s) 1590
Eco57I CTGAAG 6 cut(s) 126, 459, 1482, 1829, 2126, 2753
Eco88I CYCGRG 1 cut(s) 779
EcoICRI GAGCTC 1 cut(s) 1590
EcoO109I RGGNCCY 1 cut(s) 139
EcoRI GAATTC 2 cut(s) 914, 1546
EcoRII CCWGG 3 cut(s) 559, 2769, 2878
EcoT14I CCWWGG 4 cut(s) 526, 1064, 1884, 2514
EcoT38I GRGCYC 1 cut(s) 1592
ErhI CCWWGG 4 cut(s) 526, 1064, 1884, 2514
Esp3I CGTCTC 1 cut(s) 2898
FaeI CATG 9 cut(s) 84, 594, 1466, 1534, 1588, 1815, 2399, 2552, 2782
FalI AAGNNNNNCTT 2 cut(s) 99, 131
FaqI GGGAC 1 cut(s) 2557
FatI CATG 9 cut(s) 80, 590, 1462, 1530, 1584, 1811, 2395, 2548, 2778
FauI CCCGC 1 cut(s) 84
FbaI TGATCA 2 cut(s) 2781, 2853
FblI GTMKAC 1 cut(s) 2691
Fnu4HI GCNGC 7 cut(s) 131, 613, 941, 2077, 2324, 2370, 2530
FokI GGATG 3 cut(s) 272, 2786, 2812
FriOI GRGCYC 1 cut(s) 1592
Fsp4HI GCNGC 7 cut(s) 131, 613, 941, 2077, 2324, 2370, 2530
FspBI CTAG 5 cut(s) 1163, 1604, 2373, 2583, 2636
GlaI GCGC 1 cut(s) 2298
GluI GCNGC 7 cut(s) 131, 613, 941, 2077, 2324, 2370, 2530
GsaI CCCAGC 4 cut(s) 927, 1287, 1545, 2544
GsuI CTGGAG 1 cut(s) 2917
HaeII RGCGCY 1 cut(s) 2300
HaeIII GGCC 2 cut(s) 5, 525
HapII CCGG 6 cut(s) 201, 416, 632, 1355, 2082, 2327
HhaI GCGC 1 cut(s) 2299
Hin1II CATG 9 cut(s) 84, 594, 1466, 1534, 1588, 1815, 2399, 2552, 2782
Hin6I GCGC 1 cut(s) 2297
HinP1I GCGC 1 cut(s) 2297
HincII GTYRAC 1 cut(s) 1948
HindII GTYRAC 1 cut(s) 1948
HindIII AAGCTT 4 cut(s) 1934, 2162, 2219, 2819
HpaII CCGG 6 cut(s) 201, 416, 632, 1355, 2082, 2327
HphI GGTGA 7 cut(s) 275, 292, 787, 1427, 1643, 2003, 2863
Hpy166II GTNNAC 4 cut(s) 512, 1948, 2092, 2692
Hpy8I GTNNAC 4 cut(s) 512, 1948, 2092, 2692
Hpy99I CGWCG 1 cut(s) 2156
HpyCH4III ACNGT 6 cut(s) 97, 315, 758, 1154, 1961, 2125
HpyCH4IV ACGT 3 cut(s) 2719, 2727, 2891
HpyF10VI GCNNNNNNNGC 5 cut(s) 76, 85, 2366, 2474, 2579
HpySE526I ACGT 3 cut(s) 2719, 2727, 2891
Hsp92II CATG 9 cut(s) 84, 594, 1466, 1534, 1588, 1815, 2399, 2552, 2782
HspAI GCGC 1 cut(s) 2297
Kpn2I TCCGGA 1 cut(s) 631
KpnI GGTACC 1 cut(s) 2344
Ksp22I TGATCA 2 cut(s) 2781, 2853
LmnI GCTCC 3 cut(s) 2366, 2537, 2566
Lsp1109I GCAGC 6 cut(s) 142, 624, 952, 2088, 2335, 2541
LweI GCATC 9 cut(s) 294, 535, 952, 1776, 1886, 2012, 2017, 2850, 2860
MaeI CTAG 5 cut(s) 1163, 1604, 2373, 2583, 2636
MaeII ACGT 3 cut(s) 2719, 2727, 2891
MaeIII GTNAC 6 cut(s) 226, 252, 1221, 1253, 2587, 2638
MbiI CCGCTC 1 cut(s) 2369
MfeI CAATTG 2 cut(s) 398, 2655
MflI RGATCY 4 cut(s) 418, 1606, 1984, 2246
MhlI GDGCHC 1 cut(s) 1592
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 6 cut(s) 179, 185, 349, 574, 1543, 2595
MmeI TCCRAC 3 cut(s) 153, 385, 1359
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 631
MroXI GAANNNNTTC 1 cut(s) 2727
MscI TGGCCA 1 cut(s) 5
MseI TTAA 6 cut(s) 372, 735, 1490, 1553, 1932, 2313
MslI CAYNNNNRTG 1 cut(s) 1194
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 1211
MspI CCGG 6 cut(s) 201, 416, 632, 1355, 2082, 2327
MspR9I CCNGG 6 cut(s) 202, 416, 561, 2083, 2771, 2880
MunI CAATTG 2 cut(s) 398, 2655
MvaI CCWGG 3 cut(s) 561, 2771, 2880
MwoI GCNNNNNNNGC 5 cut(s) 76, 85, 2366, 2474, 2579
NciI CCSGG 3 cut(s) 202, 416, 2083
NlaIII CATG 9 cut(s) 84, 594, 1466, 1534, 1588, 1815, 2399, 2552, 2782
NlaIV GGNNCC 5 cut(s) 141, 342, 1438, 1638, 2342
NmuCI GTSAC 5 cut(s) 226, 252, 1221, 1253, 2587
NspI RCATGY 1 cut(s) 1815
PagI TCATGA 1 cut(s) 1584
PciI ACATGT 1 cut(s) 1811
PdmI GAANNNNTTC 1 cut(s) 2727
PfoI TCCNGGA 3 cut(s) 200, 414, 2769
PkrI GCNGC 7 cut(s) 132, 614, 942, 2078, 2325, 2371, 2531
PleI GAGTC 6 cut(s) 178, 184, 349, 573, 1542, 2594
PpsI GAGTC 6 cut(s) 178, 184, 349, 573, 1542, 2594
PpuMI RGGWCCY 1 cut(s) 139
PscI ACATGT 1 cut(s) 1811
PshAI GACNNNNGTC 1 cut(s) 1533
PsiI TTATAA 1 cut(s) 1923
Psp124BI GAGCTC 1 cut(s) 1592
Psp1406I AACGTT 1 cut(s) 2727
Psp5II RGGWCCY 1 cut(s) 139
Psp6I CCWGG 3 cut(s) 559, 2769, 2878
PspFI CCCAGC 4 cut(s) 923, 1283, 1541, 2540
PspGI CCWGG 3 cut(s) 559, 2769, 2878
PspN4I GGNNCC 5 cut(s) 141, 342, 1438, 1638, 2342
PspPI GGNCC 2 cut(s) 139, 523
PspPPI RGGWCCY 1 cut(s) 139
PstI CTGCAG 2 cut(s) 43, 2176
PstNI CAGNNNCTG 1 cut(s) 2129
PsuI RGATCY 4 cut(s) 418, 1606, 1984, 2246
PvuII CAGCTG 1 cut(s) 1211
RsaI GTAC 6 cut(s) 312, 1707, 2122, 2342, 2502, 2812
RsaNI GTAC 6 cut(s) 311, 1706, 2121, 2341, 2501, 2811
RseI CAYNNNNRTG 1 cut(s) 1194
SacI GAGCTC 1 cut(s) 1592
SaqAI TTAA 6 cut(s) 372, 735, 1490, 1553, 1932, 2313
SatI GCNGC 7 cut(s) 131, 613, 941, 2077, 2324, 2370, 2530
Sau96I GGNCC 2 cut(s) 139, 523
SchI GAGTC 6 cut(s) 179, 185, 349, 574, 1543, 2595
ScrFI CCNGG 6 cut(s) 202, 416, 561, 2083, 2771, 2880
SduI GDGCHC 1 cut(s) 1592
SfaNI GCATC 9 cut(s) 294, 535, 952, 1776, 1886, 2012, 2017, 2850, 2860
SfcI CTRYAG 2 cut(s) 39, 2172
SinI GGWCC 1 cut(s) 139
SmiMI CAYNNNNRTG 1 cut(s) 1194
SmlI CTYRAG 5 cut(s) 116, 134, 290, 866, 2630
SmoI CTYRAG 5 cut(s) 116, 134, 290, 866, 2630
SsiI CCGC 4 cut(s) 77, 1526, 2095, 2369
SspI AATATT 1 cut(s) 1778
SspMI CTAG 5 cut(s) 1163, 1604, 2373, 2583, 2636
SstI GAGCTC 1 cut(s) 1592
StyD4I CCNGG 6 cut(s) 200, 414, 559, 2081, 2769, 2878
StyI CCWWGG 4 cut(s) 526, 1064, 1884, 2514
TaaI ACNGT 6 cut(s) 97, 315, 758, 1154, 1961, 2125
TaiI ACGT 3 cut(s) 2722, 2730, 2894
TaqI TCGA 3 cut(s) 99, 710, 1770
TatI WGTACW 3 cut(s) 310, 2500, 2810
TauI GCSGC 1 cut(s) 2372
Tru1I TTAA 6 cut(s) 372, 735, 1490, 1553, 1932, 2313
Tru9I TTAA 6 cut(s) 372, 735, 1490, 1553, 1932, 2313
TscAI CASTG 3 cut(s) 235, 1732, 2596
TseFI GTSAC 5 cut(s) 226, 252, 1221, 1253, 2587
TseI GCWGC 6 cut(s) 130, 612, 940, 2076, 2323, 2529
Tsp45I GTSAC 5 cut(s) 226, 252, 1221, 1253, 2587
TspDTI ATGAA 8 cut(s) 49, 665, 909, 1660, 1820, 2049, 2408, 2775
TspRI CASTG 3 cut(s) 235, 1732, 2596
VpaK11BI GGWCC 1 cut(s) 139
XbaI TCTAGA 2 cut(s) 1162, 1603
XceI RCATGY 1 cut(s) 1815
XcmI CCANNNNNNNNNTGG 1 cut(s) 1215
XmiI GTMKAC 1 cut(s) 2691
XmnI GAANNNNTTC 1 cut(s) 2727
XspI CTAG 5 cut(s) 1163, 1604, 2373, 2583, 2636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.