Rroxscaffold_3G00232480
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009040
Physical Location & Seq
Forward (+)
18123762 .. 18129031
5270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00232480.1

Sequence Viewer

Length: 2952 bp
ATGGCCAAAATACCAGTTCCCTCCCTGCACTTGCTACCAGTCTTTACCACACTGTTCATTTCTTTGCTTTTCCCGCCATGTATGCCTCTAACGGTCGAAACTGAAGCCCTCCTTGAGTTCAAAAGGCAGCTCAAGGACCCTTTGAGTTTTCTTGATTCGTGGCAAGAGTCGGAGTCTTCTTCCCCCTGTGAGTTCTCCGGGATTACTTGCGATTCGGGGAAAGTCACTGAAATATCTCTTCACAACAAGTCACTCTCGGGTGAGATTTCGCCCTCCATTGGCATCCTCGAGAGCCTCACCACGCTTGTACTGTCTGCCAACCTCATTGCAGGAGAGGTTCCTGCCCAACTGACTCATTGTGCCAATCTTAAAACATTGAATCTGTCTGAGAATCAGTTGGTTGGAAAAATCCCGGATCTTTCCACCCTTAGAAACTTGGAGAGACTTGATCTTTCTGCCAACAGGTTCTCTTCAGAGTTCCCGTCTTGGGTGGGAAGTCTCACAGGTTTACAATATCTGGGCCTTGGGCAAAATCTGTTTGATGCAAGCGAAGTTCCTGGGAGTCTTGGAAACTTGAAGAACTTGACATGGCTGTATCTGACAAATTGCAGCTTGATGGGAGAGATTCCGGAATCTATTTACGAAATGAAGGCATTGGGAACATTTGATATGTCAAGAAACAATCTTTCTGGGAAACTCTCTAAGTCGATTTCCAAGCTACAAAACCTATTTAAGATTGAGCTGTTTGCAAACGGTTTGGGTGGAGAAATCCCACCCGAGGTTGCTAGTCTCACCCTTCTGAGGGAATTTGATATTTCAACAAACAAATTTTATGGGAAGTTGCCTTCTGAGTTCGGAAATCTCAAGAATTTAGTGGTTTTTCAGTTGTATGAAAACAATTTTTCAGGGGAATTCCCTGCTGGGTTTGGAGATATGCAGCATCTTACTGCTTTCTCAATATATGGAAACAGATTTTCTGGGGAATTTCCCGCAAATCTTGGGAAATTCTCACTTTTGGAGAGCATTGACATATCTGAGAATCAGTTTTCTGGTGCGTTCCCAAGGTTTCTTTGTGAAAAGAGGAAGCTACAATTCTTGCTCGCTTTGGAAAACAATTTTTATGGGGAGTTTCCAGATTCTTATGCTGACTGTAAATCTCTAGAGAGGTTTAGAGTAAGTAAGAACCATTTGTATGGGGAGATTCCAGCTGAGTTTTGGTCACTTCCTAAAGCGACAATCATTGAGTTTAGTGACAATAACTTTCGCGGAGTGATTTCCCCCAGCATTGGGTTTTCTACCAGCTTGAATCAGTTGATGTTGCAGAATAACAATTTCTCAGGTTATCTTCCACCAGAAATTGGGAAGCTAACAAAGTTGGACAGGCTCTATTTGAATCATAATAACTTTTCAGGTGAAATACCATCCGAAATTGGTGCCTTGAAGCAGTTATCATCTTTCCATGTTGAAGAAAATTCTTTGTCAGGCTCAATACCTTCAGAACTGGGAAACTGTGTTAGGTTGGCGGACATGAGTCTTGCTGGGAATTCCTTAACTGGAACTATCCCACCTACGCTTTCACTCATGAGCTCTTTGAACTCTCTAAATCTCTCTCGGAATAAACTCTCAGGTGAGGTTCCAGAAAATTTAGAGAAATTGAAGCTAAGTTTCATAGATTTGTCTGAAAATCTGTTATCTGGAAGAGTACCATCTGATCTTCTGACCACTGGTGGAGACAAAGCCTTTCGTGGAAACAACGAACTCTGCATCGATCAATATTCAAAAACACATTCAAATTCTGGTATGAATACATGTTCTGAAAAGCATAGTCAGAAAAGGGTGCTTCAGAATAAATTGGTTTTGTTCTCTATCATAGCATCTGCCTTGGTTGTTGTTTTAGCTGGATTGGTGCTTGTCAGTTATAAGAACTTCAAGCTTTCTGAGGTTGAAAGAGAAAACAGTTTGGAAGAGGGGAAGGAAATAGATCCGAAATGGAAACTTGCATCTTTTCACCAACTGGAAATAGATGCAGATGAAATCTGTGATTTAGAAGAAGAAAATTTGGTTGGTAGTGGCAGCACAGGGAAAGTTTACCGCCTTGATCTGAAGAAGAATGGTGGTACAGTAGCTGTAAAACAACTATGGAAAGGCGATGGCTTGAAGCTTTTGACTGCAGAGATGGATATTTTGGGAAAGATCAGGCATAGAAATATATTGAAGCTTTATGCTTGTTTAGTGAAGGGAGGATCTAATCTTCTGGTGTTTGAGTACATGGAAAATGGAAATTTGTTTCAAGCGCTTCACAGACAGCTTAAAGGCGGGCAGCCGGAATTGGATTGGTACCAGAGGTATAGGATTGCTTTGGGAGCAGCTAGGGGGATTTCTTATCTACACCATGATTGTTCCCCACCTATAATTCATAGAGATATAAAATCAACCAACATTCTGCTTGATGATGATTACGAGGCAAAAGTTGCTGATTTTGGGGTTGCAAAAATTGTACAAAACAGTCCCAAGGGTTCAGAGTGCAGCTCCTTTGCTGGGACACATGGGTATATTGCTCCTGAGCTGGCATATACTGCTAAAGTCACTGAAAAGTGCGATGTATATAGTTTTGGTGTGGTTCTACTTGAGCTAGTTACTGGTAGAAGATCGATTGAAGATGACTACGGGGAAGGAAAAGACATTGTATACTGGGTTTCCGCCAATCTGAACGACCGTGAAAACGTTCTGAAGGTTCTTGATGACAAAGTGGCAGATAGAAATATCCAGGATGACATGATCAAGGTTTTGAAGGTAGCTATTCTTTGTACTACCAAGCTTCCATCCCTGCGGCCTACAATGCGAGAGGTGATCAAGATGCTTATTGATGCTGATCCTGGCACTTTCACTTCTCCAAGCAACAATTCTGTCAAAGAAGACAAGGATTCCTCCAGTCTGCTTCTCTCCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

983

Amino Acids

108.74

Weight (kDa)

5.39

Isoelectric Point (pI)

36.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 33 - 71 3.3e-11 Leucine rich repeat N-terminal domain
LRR_4 PF12799 122 - 156 2.8e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 138 - 247 4.1e-07 Leucine-rich repeat region
LRR_8 PF13855 144 - 205 2.5e-08 Leucine rich repeat
LRR_14 PF23598 255 - 403 7.3e-09 Leucine-rich repeat region
LRR_14 PF23598 435 - 544 1.5e-07 Leucine-rich repeat region
Pkinase PF00069 683 - 952 2.4e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 685 - 954 2e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1920
Acc65I GGTACC 1 cut(s) 2337
AccB1I GGYRCC 2 cut(s) 1433, 2337
AccB7I CCANNNNNTGG 1 cut(s) 1358
AccI GTMKAC 1 cut(s) 2688
AccII CGCG 1 cut(s) 1266
AccIII TCCGGA 1 cut(s) 628
AciI CCGC 8 cut(s) 74, 990, 1266, 1523, 2092, 2316, 2700, 2830
AclI AACGTT 1 cut(s) 2724
AclWI GGATC 4 cut(s) 423, 1976, 2251, 2867
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 6 cut(s) 123, 456, 1479, 1826, 2123, 2750
AfaI GTAC 7 cut(s) 309, 1704, 2119, 2267, 2339, 2499, 2809
AfeI AGCGCT 1 cut(s) 2295
AfiI CCNNNNNNNGG 9 cut(s) 278, 487, 778, 801, 802, 1286, 1287, 1358, 2538
AflIII ACRYGT 1 cut(s) 1808
AjnI CCWGG 3 cut(s) 556, 2766, 2875
AjuI GAANNNNNNNTTGG 4 cut(s) 2046, 2078, 2887, 2919
Alw21I GWGCWC 1 cut(s) 1589
Alw26I GTCTC 4 cut(s) 436, 503, 794, 1725
AlwI GGATC 4 cut(s) 423, 1976, 2251, 2867
AlwNI CAGNNNCTG 1 cut(s) 2126
Ama87I CYCGRG 3 cut(s) 256, 287, 776
Aor13HI TCCGGA 1 cut(s) 628
Aor51HI AGCGCT 1 cut(s) 2295
AoxI GGCC 3 cut(s) 3, 520, 2831
ApeKI GCWGC 7 cut(s) 127, 609, 937, 2073, 2320, 2366, 2526
Asp700I GAANNNNTTC 1 cut(s) 2724
Asp718I GGTACC 1 cut(s) 2337
AspLEI GCGC 1 cut(s) 2296
AspS9I GGNCC 2 cut(s) 136, 520
AsuC2I CCSGG 2 cut(s) 199, 413
AsuHPI GGTGA 7 cut(s) 272, 289, 784, 1424, 1640, 2000, 2860
AvaI CYCGRG 3 cut(s) 256, 287, 776
AvaII GGWCC 1 cut(s) 136
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 1433, 2337
BanII GRGCYC 1 cut(s) 1589
BbsI GAAGAC 2 cut(s) 168, 2922
Bbv12I GWGCWC 1 cut(s) 1589
BbvI GCAGC 7 cut(s) 139, 621, 949, 2085, 2332, 2378, 2538
BccI CCATC 6 cut(s) 610, 1429, 1714, 2144, 2170, 2830
BcgI CGANNNNNNTGC 2 cut(s) 1415, 1449
BciT130I CCWGG 3 cut(s) 558, 2768, 2877
BclI TGATCA 2 cut(s) 2778, 2850
BcnI CCSGG 2 cut(s) 199, 413
BcoDI GTCTC 4 cut(s) 436, 503, 794, 1725
BfaI CTAG 4 cut(s) 786, 1160, 2370, 2633
BfmI CTRYAG 1 cut(s) 2169
BfoI RGCGCY 1 cut(s) 2297
BisI GCNGC 8 cut(s) 128, 610, 938, 2074, 2321, 2367, 2527, 2831
BlsI GCNGC 8 cut(s) 129, 611, 939, 2075, 2322, 2368, 2528, 2832
Bme1390I CCNGG 5 cut(s) 199, 413, 558, 2768, 2877
Bme18I GGWCC 1 cut(s) 136
BmeT110I CYCGRG 3 cut(s) 256, 287, 776
BmgT120I GGNCC 2 cut(s) 136, 520
BmiI GGNNCC 5 cut(s) 138, 339, 1435, 1635, 2339
BmrFI CCNGG 5 cut(s) 199, 413, 558, 2768, 2877
BmrI ACTGGG 3 cut(s) 1511, 2701, 2941
BmsI GCATC 9 cut(s) 291, 532, 949, 1773, 1883, 2009, 2014, 2847, 2857
BmuI ACTGGG 3 cut(s) 1511, 2701, 2941
BoxI GACNNNNGTC 1 cut(s) 1530
BpiI GAAGAC 2 cut(s) 168, 2922
BplI GAGNNNNNCTC 2 cut(s) 2513, 2545
BpmI CTGGAG 1 cut(s) 2914
Bpu10I CCTNAGC 1 cut(s) 2562
BpuEI CTTGAG 4 cut(s) 116, 134, 848, 2648
BpuMI CCSGG 2 cut(s) 199, 413
Bsa29I ATCGAT 2 cut(s) 1767, 2651
BsaBI GATNNNNATC 1 cut(s) 2871
BsaJI CCNNGG 6 cut(s) 523, 557, 777, 1061, 1881, 2511
BsaWI WCCGGW 1 cut(s) 628
Bsc4I CCNNNNNNNGG 9 cut(s) 278, 487, 778, 801, 802, 1286, 1287, 1358, 2538
Bse3DI GCAATG 1 cut(s) 324
Bse8I GATNNNNATC 1 cut(s) 2871
BseAI TCCGGA 1 cut(s) 628
BseBI CCWGG 3 cut(s) 558, 2768, 2877
BseCI ATCGAT 2 cut(s) 1767, 2651
BseDI CCNNGG 6 cut(s) 523, 557, 777, 1061, 1881, 2511
BseGI GGATG 4 cut(s) 282, 1421, 2776, 2822
BseJI GATNNNNATC 1 cut(s) 2871
BseLI CCNNNNNNNGG 9 cut(s) 278, 487, 778, 801, 802, 1286, 1287, 1358, 2538
BseMI GCAATG 1 cut(s) 324
BseMII CTCAG 9 cut(s) 378, 791, 840, 1026, 1200, 1350, 1638, 1929, 2553
BseXI GCAGC 7 cut(s) 139, 621, 949, 2085, 2332, 2378, 2538
BseYI CCCAGC 4 cut(s) 920, 1280, 1538, 2537
BsgI GTGCAG 2 cut(s) 11, 2545
Bsh1236I CGCG 1 cut(s) 1266
Bsh1285I CGRYCG 2 cut(s) 96, 2716
BshFI GGCC 3 cut(s) 5, 522, 2833
BshNI GGYRCC 2 cut(s) 1433, 2337
BshVI ATCGAT 2 cut(s) 1767, 2651
BsiEI CGRYCG 2 cut(s) 96, 2716
BsiHKAI GWGCWC 1 cut(s) 1589
BsiHKCI CYCGRG 3 cut(s) 256, 287, 776
BsiSI CCGG 4 cut(s) 198, 413, 629, 2324
BslFI GGGAC 2 cut(s) 2493, 2554
BslI CCNNNNNNNGG 9 cut(s) 278, 487, 778, 801, 802, 1286, 1287, 1358, 2538
BsmAI GTCTC 4 cut(s) 436, 503, 794, 1725
BsmFI GGGAC 2 cut(s) 2493, 2554
BsnI GGCC 3 cut(s) 5, 522, 2833
BsoBI CYCGRG 3 cut(s) 256, 287, 776
Bsp1286I GDGCHC 1 cut(s) 1589
Bsp13I TCCGGA 1 cut(s) 628
Bsp1407I TGTACA 1 cut(s) 2497
BspACI CCGC 8 cut(s) 74, 990, 1266, 1523, 2092, 2316, 2700, 2830
BspANI GGCC 3 cut(s) 5, 522, 2833
BspCNI CTCAG 9 cut(s) 379, 792, 841, 1027, 1201, 1349, 1637, 1930, 2554
BspDI ATCGAT 2 cut(s) 1767, 2651
BspEI TCCGGA 1 cut(s) 628
BspFNI CGCG 1 cut(s) 1266
BspHI TCATGA 1 cut(s) 1581
BspLI GGNNCC 5 cut(s) 138, 339, 1435, 1635, 2339
BspMAI CTGCAG 1 cut(s) 2173
BspPI GGATC 4 cut(s) 423, 1976, 2251, 2867
BspT107I GGYRCC 2 cut(s) 1433, 2337
BsrDI GCAATG 1 cut(s) 324
BsrGI TGTACA 1 cut(s) 2497
BssECI CCNNGG 6 cut(s) 523, 557, 777, 1061, 1881, 2511
BssNAI GTATAC 1 cut(s) 2689
BssT1I CCWWGG 4 cut(s) 523, 1061, 1881, 2511
Bst1107I GTATAC 1 cut(s) 2689
Bst2UI CCWGG 3 cut(s) 558, 2768, 2877
Bst6I CTCTTC 4 cut(s) 243, 475, 1693, 1959
BstAPI GCANNNNNTGC 2 cut(s) 2471, 2576
BstAUI TGTACA 1 cut(s) 2497
BstC8I GCNNGC 4 cut(s) 547, 1101, 2318, 2568
BstF5I GGATG 4 cut(s) 282, 1421, 2776, 2822
BstFNI CGCG 1 cut(s) 1266
BstH2I RGCGCY 1 cut(s) 2297
BstHHI GCGC 1 cut(s) 2296
BstMAI GTCTC 4 cut(s) 436, 503, 794, 1725
BstMCI CGRYCG 2 cut(s) 96, 2716
BstMWI GCNNNNNNNGC 6 cut(s) 73, 82, 2363, 2471, 2576, 2839
BstNI CCWGG 3 cut(s) 558, 2768, 2877
BstNSI RCATGY 1 cut(s) 1812
BstPAI GACNNNNGTC 1 cut(s) 1530
BstSCI CCNGG 5 cut(s) 197, 411, 556, 2766, 2875
BstSFI CTRYAG 1 cut(s) 2169
BstUI CGCG 1 cut(s) 1266
BstV1I GCAGC 7 cut(s) 139, 621, 949, 2085, 2332, 2378, 2538
BstV2I GAAGAC 2 cut(s) 168, 2922
BstX2I RGATCY 3 cut(s) 415, 1981, 2243
BstXI CCANNNNNNTGG 1 cut(s) 1193
BstYI RGATCY 3 cut(s) 415, 1981, 2243
BstZ17I GTATAC 1 cut(s) 2689
Bsu15I ATCGAT 2 cut(s) 1767, 2651
BsuRI GGCC 3 cut(s) 5, 522, 2833
BsuTUI ATCGAT 2 cut(s) 1767, 2651
BtgZI GCGATG 2 cut(s) 2163, 2613
BtsCI GGATG 4 cut(s) 282, 1421, 2776, 2822
BtsIMutI CAGTG 4 cut(s) 50, 225, 1722, 2586
Cac8I GCNNGC 4 cut(s) 547, 1101, 2318, 2568
CaiI CAGNNNCTG 1 cut(s) 2126
CciI TCATGA 1 cut(s) 1581
CfoI GCGC 1 cut(s) 2296
Cfr13I GGNCC 2 cut(s) 136, 520
ClaI ATCGAT 2 cut(s) 1767, 2651
Csp6I GTAC 7 cut(s) 308, 1703, 2118, 2266, 2338, 2498, 2808
CviQI GTAC 7 cut(s) 308, 1703, 2118, 2266, 2338, 2498, 2808
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 4 cut(s) 243, 475, 1693, 1959
EarI CTCTTC 4 cut(s) 243, 475, 1693, 1959
EciI GGCGGA 2 cut(s) 1538, 2689
Ecl136II GAGCTC 1 cut(s) 1587
Eco130I CCWWGG 4 cut(s) 523, 1061, 1881, 2511
Eco24I GRGCYC 1 cut(s) 1589
Eco47I GGWCC 1 cut(s) 136
Eco47III AGCGCT 1 cut(s) 2295
Eco53kI GAGCTC 1 cut(s) 1587
Eco57I CTGAAG 6 cut(s) 123, 456, 1479, 1826, 2123, 2750
Eco88I CYCGRG 3 cut(s) 256, 287, 776
EcoICRI GAGCTC 1 cut(s) 1587
EcoO109I RGGNCCY 1 cut(s) 136
EcoRI GAATTC 2 cut(s) 911, 1543
EcoRII CCWGG 3 cut(s) 556, 2766, 2875
EcoT14I CCWWGG 4 cut(s) 523, 1061, 1881, 2511
EcoT38I GRGCYC 1 cut(s) 1589
ErhI CCWWGG 4 cut(s) 523, 1061, 1881, 2511
FalI AAGNNNNNCTT 2 cut(s) 96, 128
FaqI GGGAC 2 cut(s) 2493, 2554
FauI CCCGC 3 cut(s) 81, 997, 2309
FbaI TGATCA 2 cut(s) 2778, 2850
FblI GTMKAC 1 cut(s) 2688
Fnu4HI GCNGC 8 cut(s) 128, 610, 938, 2074, 2321, 2367, 2527, 2831
FokI GGATG 4 cut(s) 269, 1408, 2783, 2809
FriOI GRGCYC 1 cut(s) 1589
Fsp4HI GCNGC 8 cut(s) 128, 610, 938, 2074, 2321, 2367, 2527, 2831
FspBI CTAG 4 cut(s) 786, 1160, 2370, 2633
GlaI GCGC 1 cut(s) 2295
GluI GCNGC 8 cut(s) 128, 610, 938, 2074, 2321, 2367, 2527, 2831
GsaI CCCAGC 4 cut(s) 924, 1284, 1542, 2541
GsuI CTGGAG 1 cut(s) 2914
HaeII RGCGCY 1 cut(s) 2297
HaeIII GGCC 3 cut(s) 5, 522, 2833
HapII CCGG 4 cut(s) 198, 413, 629, 2324
HhaI GCGC 1 cut(s) 2296
Hin6I GCGC 1 cut(s) 2294
HinP1I GCGC 1 cut(s) 2294
HindIII AAGCTT 4 cut(s) 1931, 2159, 2216, 2816
HpaII CCGG 4 cut(s) 198, 413, 629, 2324
HphI GGTGA 7 cut(s) 272, 289, 784, 1424, 1640, 2000, 2860
Hpy166II GTNNAC 3 cut(s) 509, 2089, 2689
Hpy8I GTNNAC 3 cut(s) 509, 2089, 2689
HpyAV CCTTC 9 cut(s) 643, 806, 855, 1503, 1966, 2230, 2666, 2725, 2785
HpyCH4IV ACGT 1 cut(s) 2724
HpyF10VI GCNNNNNNNGC 6 cut(s) 73, 82, 2363, 2471, 2576, 2839
HpySE526I ACGT 1 cut(s) 2724
HspAI GCGC 1 cut(s) 2294
Kpn2I TCCGGA 1 cut(s) 628
KpnI GGTACC 1 cut(s) 2341
Ksp22I TGATCA 2 cut(s) 2778, 2850
LmnI GCTCC 3 cut(s) 2363, 2534, 2563
Lsp1109I GCAGC 7 cut(s) 139, 621, 949, 2085, 2332, 2378, 2538
LweI GCATC 9 cut(s) 291, 532, 949, 1773, 1883, 2009, 2014, 2847, 2857
MaeI CTAG 4 cut(s) 786, 1160, 2370, 2633
MaeII ACGT 1 cut(s) 2724
MaeIII GTNAC 6 cut(s) 223, 249, 1218, 1250, 2584, 2635
MflI RGATCY 3 cut(s) 415, 1981, 2243
MhlI GDGCHC 1 cut(s) 1589
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 5 cut(s) 176, 182, 346, 571, 1540
MmeI TCCRAC 3 cut(s) 150, 382, 1356
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 628
MroXI GAANNNNTTC 1 cut(s) 2724
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 369, 732, 1550, 2310
MslI CAYNNNNRTG 1 cut(s) 1191
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 1208
MspI CCGG 4 cut(s) 198, 413, 629, 2324
MspR9I CCNGG 5 cut(s) 199, 413, 558, 2768, 2877
MvaI CCWGG 3 cut(s) 558, 2768, 2877
MvnI CGCG 1 cut(s) 1266
MwoI GCNNNNNNNGC 6 cut(s) 73, 82, 2363, 2471, 2576, 2839
NciI CCSGG 2 cut(s) 199, 413
NlaIV GGNNCC 5 cut(s) 138, 339, 1435, 1635, 2339
NmuCI GTSAC 5 cut(s) 223, 249, 1218, 1250, 2584
NspI RCATGY 1 cut(s) 1812
PaeR7I CTCGAG 1 cut(s) 287
PagI TCATGA 1 cut(s) 1581
PciI ACATGT 1 cut(s) 1808
PdmI GAANNNNTTC 1 cut(s) 2724
PflMI CCANNNNNTGG 1 cut(s) 1358
PfoI TCCNGGA 3 cut(s) 197, 411, 2766
PkrI GCNGC 8 cut(s) 129, 611, 939, 2075, 2322, 2368, 2528, 2832
PleI GAGTC 5 cut(s) 175, 181, 346, 570, 1539
PpsI GAGTC 5 cut(s) 175, 181, 346, 570, 1539
PpuMI RGGWCCY 1 cut(s) 136
PscI ACATGT 1 cut(s) 1808
PshAI GACNNNNGTC 1 cut(s) 1530
PsiI TTATAA 1 cut(s) 1920
Psp124BI GAGCTC 1 cut(s) 1589
Psp1406I AACGTT 1 cut(s) 2724
Psp5II RGGWCCY 1 cut(s) 136
Psp6I CCWGG 3 cut(s) 556, 2766, 2875
PspFI CCCAGC 4 cut(s) 920, 1280, 1538, 2537
PspGI CCWGG 3 cut(s) 556, 2766, 2875
PspN4I GGNNCC 5 cut(s) 138, 339, 1435, 1635, 2339
PspPI GGNCC 2 cut(s) 136, 520
PspPPI RGGWCCY 1 cut(s) 136
PstI CTGCAG 1 cut(s) 2173
PstNI CAGNNNCTG 1 cut(s) 2126
PsuI RGATCY 3 cut(s) 415, 1981, 2243
PvuII CAGCTG 1 cut(s) 1208
RsaI GTAC 7 cut(s) 309, 1704, 2119, 2267, 2339, 2499, 2809
RsaNI GTAC 7 cut(s) 308, 1703, 2118, 2266, 2338, 2498, 2808
RseI CAYNNNNRTG 1 cut(s) 1191
SacI GAGCTC 1 cut(s) 1589
SaqAI TTAA 4 cut(s) 369, 732, 1550, 2310
SatI GCNGC 8 cut(s) 128, 610, 938, 2074, 2321, 2367, 2527, 2831
Sau96I GGNCC 2 cut(s) 136, 520
SchI GAGTC 5 cut(s) 176, 182, 346, 571, 1540
ScrFI CCNGG 5 cut(s) 199, 413, 558, 2768, 2877
SduI GDGCHC 1 cut(s) 1589
SfaNI GCATC 9 cut(s) 291, 532, 949, 1773, 1883, 2009, 2014, 2847, 2857
SfcI CTRYAG 1 cut(s) 2169
Sfr274I CTCGAG 1 cut(s) 287
SinI GGWCC 1 cut(s) 136
SlaI CTCGAG 1 cut(s) 287
SmiMI CAYNNNNRTG 1 cut(s) 1191
SmlI CTYRAG 5 cut(s) 113, 131, 287, 863, 2627
SmoI CTYRAG 5 cut(s) 113, 131, 287, 863, 2627
SsiI CCGC 8 cut(s) 74, 990, 1266, 1523, 2092, 2316, 2700, 2830
SspI AATATT 1 cut(s) 1775
SspMI CTAG 4 cut(s) 786, 1160, 2370, 2633
SstI GAGCTC 1 cut(s) 1589
StyD4I CCNGG 5 cut(s) 197, 411, 556, 2766, 2875
StyI CCWWGG 4 cut(s) 523, 1061, 1881, 2511
TaiI ACGT 1 cut(s) 2727
TaqI TCGA 5 cut(s) 96, 288, 707, 1767, 2651
TatI WGTACW 4 cut(s) 307, 2265, 2497, 2807
TauI GCSGC 1 cut(s) 2833
Tru1I TTAA 4 cut(s) 369, 732, 1550, 2310
Tru9I TTAA 4 cut(s) 369, 732, 1550, 2310
TscAI CASTG 4 cut(s) 57, 232, 1729, 2593
TseFI GTSAC 5 cut(s) 223, 249, 1218, 1250, 2584
TseI GCWGC 7 cut(s) 127, 609, 937, 2073, 2320, 2366, 2526
Tsp45I GTSAC 5 cut(s) 223, 249, 1218, 1250, 2584
TspDTI ATGAA 7 cut(s) 46, 662, 906, 1657, 1817, 2046, 2405
TspRI CASTG 4 cut(s) 57, 232, 1729, 2593
Van91I CCANNNNNTGG 1 cut(s) 1358
VpaK11BI GGWCC 1 cut(s) 136
XbaI TCTAGA 1 cut(s) 1159
XceI RCATGY 1 cut(s) 1812
XcmI CCANNNNNNNNNTGG 1 cut(s) 1212
XhoI CTCGAG 1 cut(s) 287
XmiI GTMKAC 1 cut(s) 2688
XmnI GAANNNNTTC 1 cut(s) 2724
XspI CTAG 4 cut(s) 786, 1160, 2370, 2633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.