MD08G1228700.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010481
Physical Location & Seq
Forward (+)
29513337 .. 29515845
2509 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1228700.v1.1.491

Sequence Viewer

Length: 1215 bp
ATGGATAACGCCAAAGACGTTGTAATTTGTAATTGTGTATCCAAGAAATGGAAGGATTCAATGCCTTATATAAGGAGCCTTTACTTCCCTAGAAGCTCCTTTGATAGCCGTAAGGGCGGAGACAGCCCTGATGACATTGTATTGAAGATGGTTTCAGCGATTGAACGGTTAGAAAATCTGGTTGTTTATAGTCCCTTCTCCGGTGCTGGCCTTGCTTCGTGGTTATCAATCATTGGTCCCTCTCTTAAGTATCTGGAGCTCCGAATGGACAATCTTGCTGATTATCAGGCCTGTCTTGAAAGGCCTTCAAAATTGGATTACTTAACTGCTGCACAAAATTTGGAGTCTTTAAAACTTTGGGGAGTTTTAATGGCCCGTTCACCAAAGTGGGATGTCTTCCAGAAACTGAAGAACCTTGAAATTGTTGGAGCAAAACTGGAGGATCCTGCGTTGTCAACTGTACTTCAGGCCTGCCCTAATCTCACCAATTTGGTGTTACTTGGTTGTGAAGGAGTGAGATTGGTTTCGATTGAGTTGCCACATTTGGAGCAGTGTAAGCTGGACTTCTATGGCTCGGGCAACTGCTCACTTTCCATGGTTTGCCCGAAAATTAAAGTCCTTGAAGTGCAAGGCTGTAGCTGGATTCGGGTTCGTGATGCAAAGCATTTGTGCAAACTTTCAATTGCCAATAATGCAGGGAGAGTCTACATGGTCGATTTCGAGAAACTTGTTGCTCTAGAGTTCTTATCCATTAGGGGAGTCCAGTGGTGTTGGGACGCAATAAGAAAAATGCTTCAATGGGCAAGTGAAGTAAAGCACCTTTATATGAAGGTGGAATTCACGGGGGATTTCGAGACCCTTCAACCCTTTCCAGAGGTTGACTTCGTTGATTTCTTCAATAACCATCCCAGGCTGCAAAAGTTTGATATCCATGGAGCCATGTTTGCTGCCCTCTGTCAAAAGAACAGCCTGAAAAATGTTGATTCGGGATTTGCAATTCCTTGCCTGGAAGAAGTGATGATCACAGTGAGATCGCCGCTGAATGCTGAACAGAAAATGAGCACTCTTGAGTCCTTGTTGAAGTACGGGAAGAATCTGAAGACAATGGTGATAAAGATTCTTCAGATGAAGAGCAGTCACAGCAGTGCAGATGATTTCTTTGACGAGATTTGCAGGTTTAGATACATGAACCGAAAGATCGTTAGAATAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0009506 GO:0030054 GO:0055044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

405

Amino Acids

46.02

Weight (kDa)

8.29

Isoelectric Point (pI)

42.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 108 - 185 1.8e-06 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010481)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10780 AT1G10780
fragaria_vesca FvH4_5g29550 FvH4_5g29550
malus_domestica MD08G1228700.v1.1 MD15G1422700.v1.1
prunus_persica Prupe.1G568000_v2.0.a1
pyrus_communis pycom15g37460
rosa_chinensis RchiOBHm_Chr7g0222501
rosa_laevigata RLG00000002049
rosa_multiflora Rmu_ssc0000146.1_g000020
rosa_roxburghii Rroxscaffold_3G00236730
rosa_rugosa Rorug07G0209900 Rorug07G0210000 Rorug07G0210000
rosa_samantha Rh7AG351700 Rh7BG342300 Rh7CG369100 Rh7DG348400
rosa_wichuraiana Rw7G029690 Rw7G030020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1164
AccB7I CCANNNNNTGG 1 cut(s) 48
AccI GTMKAC 1 cut(s) 705
AciI CCGC 2 cut(s) 117, 1037
AclWI GGATC 2 cut(s) 437, 450
AcsI RAATTY 2 cut(s) 337, 836
AcuI CTGAAG 4 cut(s) 428, 449, 1106, 1118
AfaI GTAC 2 cut(s) 462, 1085
AfiI CCNNNNNNNGG 2 cut(s) 48, 200
AflII CTTAAG 1 cut(s) 245
AjnI CCWGG 2 cut(s) 908, 1005
AleI CACNNNNGTG 2 cut(s) 385, 1143
AluBI AGCT 4 cut(s) 96, 259, 559, 639
AluI AGCT 4 cut(s) 96, 259, 559, 639
Alw21I GWGCWC 2 cut(s) 261, 1064
Alw26I GTCTC 2 cut(s) 114, 848
AlwI GGATC 2 cut(s) 437, 450
AlwNI CAGNNNCTG 1 cut(s) 406
Ama87I CYCGRG 1 cut(s) 574
AoxI GGCC 5 cut(s) 208, 288, 302, 372, 468
ApeKI GCWGC 3 cut(s) 329, 913, 947
ApoI RAATTY 2 cut(s) 337, 836
AspS9I GGNCC 2 cut(s) 236, 373
AsuHPI GGTGA 3 cut(s) 372, 475, 1120
AvaI CYCGRG 1 cut(s) 574
AvaII GGWCC 1 cut(s) 236
BamHI GGATCC 1 cut(s) 442
BanII GRGCYC 1 cut(s) 261
BbsI GAAGAC 2 cut(s) 388, 1106
Bbv12I GWGCWC 2 cut(s) 261, 1064
BbvI GCAGC 3 cut(s) 316, 900, 934
BccI CCATC 2 cut(s) 142, 912
BceAI ACGGC 1 cut(s) 93
BcgI CGANNNNNNTGC 2 cut(s) 517, 551
BciT130I CCWGG 2 cut(s) 910, 1007
BciVI GTATCC 1 cut(s) 49
BclI TGATCA 1 cut(s) 1020
BcoDI GTCTC 2 cut(s) 114, 848
BfaI CTAG 2 cut(s) 90, 737
BfmI CTRYAG 1 cut(s) 634
BfrI CTTAAG 1 cut(s) 245
BfuAI ACCTGC 1 cut(s) 1164
BfuI GTATCC 1 cut(s) 49
BglI GCCNNNNNGGC 1 cut(s) 114
BisI GCNGC 4 cut(s) 330, 914, 948, 1037
BlsI GCNGC 4 cut(s) 331, 915, 949, 1038
Bme1390I CCNGG 2 cut(s) 910, 1007
Bme18I GGWCC 1 cut(s) 236
BmeT110I CYCGRG 1 cut(s) 574
BmgT120I GGNCC 2 cut(s) 236, 373
BmiI GGNNCC 4 cut(s) 77, 238, 444, 937
BmrFI CCNGG 2 cut(s) 910, 1007
BmsI GCATC 1 cut(s) 646
BpiI GAAGAC 2 cut(s) 388, 1106
BpmI CTGGAG 2 cut(s) 275, 458
BpuEI CTTGAG 1 cut(s) 1088
BsaI GGTCTC 1 cut(s) 848
BsaJI CCNNGG 3 cut(s) 594, 908, 931
BsaWI WCCGGW 1 cut(s) 200
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 200
Bse1I ACTGG 2 cut(s) 441, 763
BseBI CCWGG 2 cut(s) 910, 1007
BseDI CCNNGG 3 cut(s) 594, 908, 931
BseGI GGATG 2 cut(s) 397, 904
BseLI CCNNNNNNNGG 2 cut(s) 48, 200
BseNI ACTGG 2 cut(s) 441, 763
BseXI GCAGC 3 cut(s) 316, 900, 934
BsgI GTGCAG 2 cut(s) 315, 1167
BshFI GGCC 5 cut(s) 210, 290, 304, 374, 470
BsiHKAI GWGCWC 2 cut(s) 261, 1064
BsiHKCI CYCGRG 1 cut(s) 574
BsiSI CCGG 1 cut(s) 201
BslFI GGGAC 3 cut(s) 177, 222, 788
BslI CCNNNNNNNGG 2 cut(s) 48, 200
BsmAI GTCTC 2 cut(s) 114, 848
BsmFI GGGAC 3 cut(s) 177, 222, 788
BsmI GAATGC 1 cut(s) 1048
BsnI GGCC 5 cut(s) 210, 290, 304, 374, 470
Bso31I GGTCTC 1 cut(s) 848
BsoBI CYCGRG 1 cut(s) 574
Bsp1286I GDGCHC 2 cut(s) 261, 1064
Bsp143I GATC 4 cut(s) 442, 1020, 1031, 1197
Bsp19I CCATGG 2 cut(s) 594, 931
BspACI CCGC 2 cut(s) 117, 1037
BspANI GGCC 5 cut(s) 210, 290, 304, 374, 470
BspLI GGNNCC 4 cut(s) 77, 238, 444, 937
BspMI ACCTGC 1 cut(s) 1164
BspPI GGATC 2 cut(s) 437, 450
BspQI GCTCTTC 1 cut(s) 1124
BspTI CTTAAG 1 cut(s) 245
BspTNI GGTCTC 1 cut(s) 848
BsrI ACTGG 2 cut(s) 441, 763
BssECI CCNNGG 3 cut(s) 594, 908, 931
BssMI GATC 4 cut(s) 442, 1020, 1031, 1197
BssT1I CCWWGG 2 cut(s) 594, 931
Bst2UI CCWGG 2 cut(s) 910, 1007
Bst4CI ACNGT 3 cut(s) 168, 460, 1027
Bst6I CTCTTC 1 cut(s) 1124
BstAFI CTTAAG 1 cut(s) 245
BstC8I GCNNGC 2 cut(s) 208, 472
BstDSI CCRYGG 2 cut(s) 594, 931
BstF5I GGATG 2 cut(s) 397, 904
BstKTI GATC 4 cut(s) 445, 1023, 1034, 1200
BstMAI GTCTC 2 cut(s) 114, 848
BstMBI GATC 4 cut(s) 442, 1020, 1031, 1197
BstMWI GCNNNNNNNGC 7 cut(s) 114, 123, 212, 556, 692, 944, 1140
BstNI CCWGG 2 cut(s) 910, 1007
BstSCI CCNGG 2 cut(s) 908, 1005
BstSFI CTRYAG 1 cut(s) 634
BstV1I GCAGC 3 cut(s) 316, 900, 934
BstV2I GAAGAC 2 cut(s) 388, 1106
BstX2I RGATCY 1 cut(s) 442
BstYI RGATCY 1 cut(s) 442
BsuI GTATCC 1 cut(s) 49
BsuRI GGCC 5 cut(s) 210, 290, 304, 374, 470
BtgI CCRYGG 2 cut(s) 594, 931
BtsCI GGATG 2 cut(s) 397, 904
BtsI GCAGTG 2 cut(s) 557, 1150
BtsIMutI CAGTG 4 cut(s) 557, 770, 1032, 1150
BveI ACCTGC 1 cut(s) 1164
Cac8I GCNNGC 2 cut(s) 208, 472
CaiI CAGNNNCTG 1 cut(s) 406
Cfr13I GGNCC 2 cut(s) 236, 373
CseI GACGC 1 cut(s) 785
Csp6I GTAC 2 cut(s) 461, 1084
CviAII CATG 5 cut(s) 595, 709, 932, 940, 1186
CviQI GTAC 2 cut(s) 461, 1084
DpnI GATC 4 cut(s) 444, 1022, 1033, 1199
DpnII GATC 4 cut(s) 442, 1020, 1031, 1197
DraI TTTAAA 1 cut(s) 351
Eam1104I CTCTTC 1 cut(s) 1124
EarI CTCTTC 1 cut(s) 1124
EciI GGCGGA 1 cut(s) 132
Ecl136II GAGCTC 1 cut(s) 259
Eco130I CCWWGG 2 cut(s) 594, 931
Eco147I AGGCCT 3 cut(s) 290, 304, 470
Eco24I GRGCYC 1 cut(s) 261
Eco31I GGTCTC 1 cut(s) 848
Eco32I GATATC 1 cut(s) 928
Eco47I GGWCC 1 cut(s) 236
Eco53kI GAGCTC 1 cut(s) 259
Eco57I CTGAAG 4 cut(s) 428, 449, 1106, 1118
Eco88I CYCGRG 1 cut(s) 574
EcoICRI GAGCTC 1 cut(s) 259
EcoRI GAATTC 1 cut(s) 836
EcoRII CCWGG 2 cut(s) 908, 1005
EcoRV GATATC 1 cut(s) 928
EcoT14I CCWWGG 2 cut(s) 594, 931
EcoT38I GRGCYC 1 cut(s) 261
ErhI CCWWGG 2 cut(s) 594, 931
FaeI CATG 5 cut(s) 598, 712, 935, 943, 1189
FalI AAGNNNNNCTT 2 cut(s) 548, 580
FaqI GGGAC 3 cut(s) 177, 222, 788
FatI CATG 5 cut(s) 594, 708, 931, 939, 1185
FbaI TGATCA 1 cut(s) 1020
FblI GTMKAC 1 cut(s) 705
Fnu4HI GCNGC 4 cut(s) 330, 914, 948, 1037
FokI GGATG 2 cut(s) 404, 891
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 4 cut(s) 330, 914, 948, 1037
FspBI CTAG 2 cut(s) 90, 737
GluI GCNGC 4 cut(s) 330, 914, 948, 1037
GsuI CTGGAG 2 cut(s) 275, 458
HaeIII GGCC 5 cut(s) 210, 290, 304, 374, 470
HapII CCGG 1 cut(s) 201
HgaI GACGC 1 cut(s) 785
Hin1II CATG 5 cut(s) 598, 712, 935, 943, 1189
HincII GTYRAC 2 cut(s) 456, 880
HindII GTYRAC 2 cut(s) 456, 880
HinfI GANTC 9 cut(s) 56, 344, 643, 702, 759, 983, 1070, 1093, 1117
HpaII CCGG 1 cut(s) 201
HphI GGTGA 3 cut(s) 372, 475, 1120
Hpy166II GTNNAC 4 cut(s) 380, 456, 706, 880
Hpy188I TCNGA 3 cut(s) 263, 1098, 1125
Hpy8I GTNNAC 4 cut(s) 380, 456, 706, 880
HpyAV CCTTC 6 cut(s) 46, 205, 315, 503, 823, 869
HpyCH4III ACNGT 3 cut(s) 168, 460, 1027
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 9 cut(s) 332, 628, 659, 672, 695, 916, 995, 1148, 1173
HpyF10VI GCNNNNNNNGC 7 cut(s) 114, 123, 212, 556, 692, 944, 1140
HpySE526I ACGT 1 cut(s) 18
Hsp92II CATG 5 cut(s) 598, 712, 935, 943, 1189
Ksp22I TGATCA 1 cut(s) 1020
Kzo9I GATC 4 cut(s) 442, 1020, 1031, 1197
LguI GCTCTTC 1 cut(s) 1124
LmnI GCTCC 7 cut(s) 75, 101, 256, 264, 428, 547, 935
Lsp1109I GCAGC 3 cut(s) 316, 900, 934
LweI GCATC 1 cut(s) 646
MaeI CTAG 2 cut(s) 90, 737
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 2 cut(s) 495, 1136
MalI GATC 4 cut(s) 444, 1022, 1033, 1199
MboI GATC 4 cut(s) 442, 1020, 1031, 1197
MboII GAAGA 9 cut(s) 157, 388, 421, 886, 1022, 1102, 1111, 1112, 1141
MfeI CAATTG 1 cut(s) 681
MflI RGATCY 1 cut(s) 442
MhlI GDGCHC 2 cut(s) 261, 1064
MlyI GAGTC 4 cut(s) 353, 711, 768, 1079
MmeI TCCRAC 1 cut(s) 406
MnlI CCTC 4 cut(s) 250, 433, 868, 962
MseI TTAA 5 cut(s) 246, 323, 350, 368, 612
MslI CAYNNNNRTG 2 cut(s) 385, 1143
MspA1I CMGCKG 1 cut(s) 1039
MspCI CTTAAG 1 cut(s) 245
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 2 cut(s) 910, 1007
MunI CAATTG 1 cut(s) 681
Mva1269I GAATGC 1 cut(s) 1048
MvaI CCWGG 2 cut(s) 910, 1007
MwoI GCNNNNNNNGC 7 cut(s) 114, 123, 212, 556, 692, 944, 1140
NcoI CCATGG 2 cut(s) 594, 931
NdeII GATC 4 cut(s) 442, 1020, 1031, 1197
NlaIII CATG 5 cut(s) 598, 712, 935, 943, 1189
NlaIV GGNNCC 4 cut(s) 77, 238, 444, 937
NmuCI GTSAC 1 cut(s) 1136
OliI CACNNNNGTG 2 cut(s) 385, 1143
PceI AGGCCT 3 cut(s) 290, 304, 470
PciSI GCTCTTC 1 cut(s) 1124
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PctI GAATGC 1 cut(s) 1048
PfeI GAWTC 5 cut(s) 56, 643, 983, 1093, 1117
PflMI CCANNNNNTGG 1 cut(s) 48
PkrI GCNGC 4 cut(s) 331, 915, 949, 1038
PleI GAGTC 4 cut(s) 352, 710, 767, 1078
PpsI GAGTC 4 cut(s) 352, 710, 767, 1078
Psp124BI GAGCTC 1 cut(s) 261
Psp6I CCWGG 2 cut(s) 908, 1005
PspGI CCWGG 2 cut(s) 908, 1005
PspN4I GGNNCC 4 cut(s) 77, 238, 444, 937
PspPI GGNCC 2 cut(s) 236, 373
PstNI CAGNNNCTG 1 cut(s) 406
PsuI RGATCY 1 cut(s) 442
RsaI GTAC 2 cut(s) 462, 1085
RsaNI GTAC 2 cut(s) 461, 1084
RseI CAYNNNNRTG 2 cut(s) 385, 1143
SacI GAGCTC 1 cut(s) 261
SapI GCTCTTC 1 cut(s) 1124
SaqAI TTAA 5 cut(s) 246, 323, 350, 368, 612
SatI GCNGC 4 cut(s) 330, 914, 948, 1037
Sau3AI GATC 4 cut(s) 442, 1020, 1031, 1197
Sau96I GGNCC 2 cut(s) 236, 373
SchI GAGTC 4 cut(s) 353, 711, 768, 1079
ScrFI CCNGG 2 cut(s) 910, 1007
SduI GDGCHC 2 cut(s) 261, 1064
SfaNI GCATC 1 cut(s) 646
SfcI CTRYAG 1 cut(s) 634
SinI GGWCC 1 cut(s) 236
SmiMI CAYNNNNRTG 2 cut(s) 385, 1143
SmlI CTYRAG 2 cut(s) 245, 1067
SmoI CTYRAG 2 cut(s) 245, 1067
SseBI AGGCCT 3 cut(s) 290, 304, 470
SsiI CCGC 2 cut(s) 117, 1037
SspMI CTAG 2 cut(s) 90, 737
SstI GAGCTC 1 cut(s) 261
StuI AGGCCT 3 cut(s) 290, 304, 470
StyD4I CCNGG 2 cut(s) 908, 1005
StyI CCWWGG 2 cut(s) 594, 931
TaaI ACNGT 3 cut(s) 168, 460, 1027
TaiI ACGT 1 cut(s) 21
TaqI TCGA 4 cut(s) 527, 714, 720, 852
TatI WGTACW 1 cut(s) 460
TauI GCSGC 1 cut(s) 1039
TfiI GAWTC 5 cut(s) 56, 643, 983, 1093, 1117
Tru1I TTAA 5 cut(s) 246, 323, 350, 368, 612
Tru9I TTAA 5 cut(s) 246, 323, 350, 368, 612
TscAI CASTG 4 cut(s) 557, 770, 1032, 1150
TseFI GTSAC 1 cut(s) 1136
TseI GCWGC 3 cut(s) 329, 913, 947
Tsp45I GTSAC 1 cut(s) 1136
TspDTI ATGAA 3 cut(s) 842, 1142, 1202
TspRI CASTG 4 cut(s) 557, 770, 1032, 1150
Van91I CCANNNNNTGG 1 cut(s) 48
Vha464I CTTAAG 1 cut(s) 245
VpaK11BI GGWCC 1 cut(s) 236
XapI RAATTY 2 cut(s) 337, 836
XbaI TCTAGA 1 cut(s) 736
XmiI GTMKAC 1 cut(s) 705
XspI CTAG 2 cut(s) 90, 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.