RLG00000002049

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0010481
Physical Location & Seq
Forward (+)
24733167 .. 24735925
2759 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002049

Sequence Viewer

Length: 1260 bp
ATGGAGTCTCTTCCAGATGCTATTGTTCAATACATATTGTCGTACATGAATAATGCGCGAGATGTGGCACTTTGTAATTGTGTATCCAAGCGATGGAAGGACTCAATTCCATATATACGGAGCCTTTTCTTCCCCAGGAACTCCTTTGATAATCTCACAGGCAGTGACACTCCTGATGACATTGTATTGAAGATGATTTCTCTGATTGAAAGATTGGAAAATCTTGTTGTTTATAGCCCATTTTCGGGTGCTGGGCTTGCTTCATGGTTGACGCTGATAGGCCCCTCCCTTAGGTATCTTGAGCTTCGAATGGACAATCTTGCTGAACAAGAGGTCTGCAATGAACGCCCTTCGAAATTGGATTGCTTAACTGCTGCAAAGAGTTTGGAGTCTTTAAAACTTTGGGGAGTCTTAATGACCCGTTCCCCTAAGTGGGATGTCTTCCAGAAACTCAAGAACCTTGAAATTGTTGGTGCAAGGTTGGAGGATCCTGCGCTGTCAACCGTGCTTCAGGCCTGTCCTAATCTGACCAAACTGTTGCTGCTTGGTTGCGAAGGAGTCGTTGCAGTTTCAATTGAGTTGCCACATCTGGAGCAGTGTAAGCTAGACTTCTACGGCTTGGGCAATTGCTCATTTTCTATGAACTGCCCAAAAATTGAGGTCCTTGAGGTGCAAGGATGTAGTTGGATTCGGGTTCAGAAGACAAAGCACTTGAGGAATCTTTCAATTGCCAATAATGCAGGGAGAGTCTACTTGGTTGATTTTGAAAAACTTGTGGCTCTGGAGTCCTTATCCATTAGGGGCGTCCAATGGTGTTGGGGTGCAATAAGCAGTATGCTGCAGTGGGCAAGCGAAGTAAAGCACCTCTATATGAAGGTGGAATTCACAGGAGATTTTGAGACTCTTCAACCCTTTCCAGAGGTTGACTTTGTTGATTTTTTTAATAGTCATCCAAAGCTGCAAAATTTTGACATCCATGGAGCCATGTTTGCAGCTCTTTGTCAGAAGAACAGTCTGAAAAATGTTGATTCAGGATTTCTAATTCCTTGTCTGGAGTCAGTGGTGATCACAGTGCGATCACCACTGAATGCTGAACAGAAAATGAGCACTCTTGAGTCCTTGTTGAGTTATGGGAAGAATCTCAAGAGCATGGTGATCAAGGTTCTTCAGATGAAGAGTAACCATAGCAGCGCAGATGATTTCTTTGATGAGATATGCAGGTTTACATGCATGAACCGAAAGATAGTTAGAATAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0009506 GO:0030054 GO:0055044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

420

Amino Acids

47.39

Weight (kDa)

6.15

Isoelectric Point (pI)

40.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 2 - 36 5.4e-06 F-box-like
LRR_At1g61320_AtMIF1 PF23622 85 - 326 3.6e-06 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 121 - 221 3.4e-06 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010481)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10780 AT1G10780
fragaria_vesca FvH4_5g29550 FvH4_5g29550
malus_domestica MD08G1228700.v1.1 MD15G1422700.v1.1
prunus_persica Prupe.1G568000_v2.0.a1
pyrus_communis pycom15g37460
rosa_chinensis RchiOBHm_Chr7g0222501
rosa_laevigata RLG00000002049
rosa_multiflora Rmu_ssc0000146.1_g000020
rosa_roxburghii Rroxscaffold_3G00236730
rosa_rugosa Rorug07G0209900 Rorug07G0210000 Rorug07G0210000
rosa_samantha Rh7AG351700 Rh7BG342300 Rh7CG369100 Rh7DG348400
rosa_wichuraiana Rw7G029690 Rw7G030020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1209
AccB7I CCANNNNNTGG 1 cut(s) 93
AccI GTMKAC 1 cut(s) 750
AccII CGCG 1 cut(s) 58
AclWI GGATC 2 cut(s) 482, 495
AcsI RAATTY 2 cut(s) 881, 964
AcuI CTGAAG 2 cut(s) 494, 1151
AcyI GRCGYC 1 cut(s) 804
AfaI GTAC 1 cut(s) 44
AfiI CCNNNNNNNGG 6 cut(s) 93, 244, 245, 291, 432, 433
AgsI TTSAA 8 cut(s) 29, 190, 209, 464, 573, 726, 767, 908
AjnI CCWGG 1 cut(s) 134
AluBI AGCT 4 cut(s) 304, 604, 958, 995
AluI AGCT 4 cut(s) 304, 604, 958, 995
Alw21I GWGCWC 1 cut(s) 1109
Alw26I GTCTC 2 cut(s) 12, 893
AlwI GGATC 2 cut(s) 482, 495
AoxI GGCC 2 cut(s) 280, 513
ApeKI GCWGC 6 cut(s) 374, 541, 838, 958, 992, 1188
ApoI RAATTY 2 cut(s) 881, 964
AspLEI GCGC 3 cut(s) 58, 496, 1193
AspS9I GGNCC 2 cut(s) 281, 661
AsuHPI GGTGA 3 cut(s) 1071, 1075, 1165
AsuII TTCGAA 2 cut(s) 307, 353
AvaII GGWCC 1 cut(s) 661
AxyI CCTNAGG 1 cut(s) 290
BamHI GGATCC 1 cut(s) 487
BbsI GAAGAC 2 cut(s) 433, 707
Bbv12I GWGCWC 1 cut(s) 1109
BbvI GCAGC 6 cut(s) 361, 528, 825, 945, 1004, 1200
BccI CCATC 1 cut(s) 87
BceAI ACGGC 1 cut(s) 631
BciT130I CCWGG 1 cut(s) 136
BciVI GTATCC 1 cut(s) 94
BclI TGATCA 2 cut(s) 1065, 1155
BcoDI GTCTC 2 cut(s) 12, 893
BfaI CTAG 1 cut(s) 605
BfmI CTRYAG 1 cut(s) 839
BfuAI ACCTGC 1 cut(s) 1209
BfuI GTATCC 1 cut(s) 94
BisI GCNGC 6 cut(s) 375, 542, 839, 959, 993, 1189
BlsI GCNGC 6 cut(s) 376, 543, 840, 960, 994, 1190
Bme1390I CCNGG 1 cut(s) 136
Bme18I GGWCC 1 cut(s) 661
BmgT120I GGNCC 2 cut(s) 281, 661
BmiI GGNNCC 4 cut(s) 122, 283, 489, 982
BmrFI CCNGG 1 cut(s) 136
BmsI GCATC 1 cut(s) 7
BpiI GAAGAC 2 cut(s) 433, 707
BpmI CTGGAG 3 cut(s) 611, 803, 1073
Bpu14I TTCGAA 2 cut(s) 307, 353
BpuEI CTTGAG 6 cut(s) 320, 437, 686, 733, 1127, 1133
BsaHI GRCGYC 1 cut(s) 804
BsaJI CCNNGG 2 cut(s) 134, 976
Bsc4I CCNNNNNNNGG 6 cut(s) 93, 244, 245, 291, 432, 433
Bse21I CCTNAGG 1 cut(s) 290
Bse3DI GCAATG 1 cut(s) 346
BseBI CCWGG 1 cut(s) 136
BseDI CCNNGG 2 cut(s) 134, 976
BseGI GGATG 4 cut(s) 442, 683, 949, 972
BseLI CCNNNNNNNGG 6 cut(s) 93, 244, 245, 291, 432, 433
BseMI GCAATG 1 cut(s) 346
BseXI GCAGC 6 cut(s) 361, 528, 825, 945, 1004, 1200
BseYI CCCAGC 1 cut(s) 251
Bsh1236I CGCG 1 cut(s) 58
BshFI GGCC 2 cut(s) 282, 515
BsiHKAI GWGCWC 1 cut(s) 1109
BslI CCNNNNNNNGG 6 cut(s) 93, 244, 245, 291, 432, 433
BsmAI GTCTC 2 cut(s) 12, 893
BsmI GAATGC 1 cut(s) 1093
BsnI GGCC 2 cut(s) 282, 515
Bsp119I TTCGAA 2 cut(s) 307, 353
Bsp1286I GDGCHC 1 cut(s) 1109
Bsp143I GATC 4 cut(s) 487, 1065, 1076, 1155
Bsp19I CCATGG 1 cut(s) 976
BspANI GGCC 2 cut(s) 282, 515
BspFNI CGCG 1 cut(s) 58
BspLI GGNNCC 4 cut(s) 122, 283, 489, 982
BspMAI CTGCAG 1 cut(s) 843
BspMI ACCTGC 1 cut(s) 1209
BspPI GGATC 2 cut(s) 482, 495
BspT104I TTCGAA 2 cut(s) 307, 353
BsrDI GCAATG 1 cut(s) 346
BssECI CCNNGG 2 cut(s) 134, 976
BssMI GATC 4 cut(s) 487, 1065, 1076, 1155
BssNI GRCGYC 1 cut(s) 804
BssT1I CCWWGG 1 cut(s) 976
Bst2UI CCWGG 1 cut(s) 136
Bst4CI ACNGT 4 cut(s) 505, 537, 1013, 1072
Bst6I CTCTTC 3 cut(s) 15, 909, 1169
BstACI GRCGYC 1 cut(s) 804
BstBI TTCGAA 2 cut(s) 307, 353
BstC8I GCNNGC 2 cut(s) 258, 850
BstDEI CTNAG 2 cut(s) 290, 429
BstDSI CCRYGG 1 cut(s) 976
BstENI CCTNNNNNAGG 1 cut(s) 289
BstF5I GGATG 4 cut(s) 442, 683, 949, 972
BstFNI CGCG 1 cut(s) 58
BstHHI GCGC 3 cut(s) 58, 496, 1193
BstKTI GATC 4 cut(s) 490, 1068, 1079, 1158
BstMAI GTCTC 2 cut(s) 12, 893
BstMBI GATC 4 cut(s) 487, 1065, 1076, 1155
BstMWI GCNNNNNNNGC 5 cut(s) 257, 345, 601, 737, 989
BstNI CCWGG 1 cut(s) 136
BstNSI RCATGY 1 cut(s) 1230
BstSCI CCNGG 1 cut(s) 134
BstSFI CTRYAG 1 cut(s) 839
BstUI CGCG 1 cut(s) 58
BstV1I GCAGC 6 cut(s) 361, 528, 825, 945, 1004, 1200
BstV2I GAAGAC 2 cut(s) 433, 707
BstX2I RGATCY 1 cut(s) 487
BstYI RGATCY 1 cut(s) 487
Bsu36I CCTNAGG 1 cut(s) 290
BsuI GTATCC 1 cut(s) 94
BsuRI GGCC 2 cut(s) 282, 515
BtgI CCRYGG 1 cut(s) 976
BtgZI GCGATG 1 cut(s) 106
BtsCI GGATG 4 cut(s) 442, 683, 949, 972
BtsI GCAGTG 3 cut(s) 169, 602, 848
BtsIMutI CAGTG 6 cut(s) 169, 602, 848, 1065, 1077, 1082
BveI ACCTGC 1 cut(s) 1209
Cac8I GCNNGC 2 cut(s) 258, 850
CfoI GCGC 3 cut(s) 58, 496, 1193
Cfr13I GGNCC 2 cut(s) 281, 661
CseI GACGC 2 cut(s) 280, 793
Csp6I GTAC 1 cut(s) 43
CviAII CATG 7 cut(s) 46, 264, 977, 985, 1150, 1227, 1231
CviQI GTAC 1 cut(s) 43
DdeI CTNAG 2 cut(s) 290, 429
DpnI GATC 4 cut(s) 489, 1067, 1078, 1157
DpnII GATC 4 cut(s) 487, 1065, 1076, 1155
DraI TTTAAA 1 cut(s) 396
Eam1104I CTCTTC 3 cut(s) 15, 909, 1169
EarI CTCTTC 3 cut(s) 15, 909, 1169
Eco130I CCWWGG 1 cut(s) 976
Eco147I AGGCCT 1 cut(s) 515
Eco47I GGWCC 1 cut(s) 661
Eco57I CTGAAG 2 cut(s) 494, 1151
Eco81I CCTNAGG 1 cut(s) 290
EcoNI CCTNNNNNAGG 1 cut(s) 289
EcoO109I RGGNCCY 2 cut(s) 281, 661
EcoRI GAATTC 1 cut(s) 881
EcoRII CCWGG 1 cut(s) 134
EcoT14I CCWWGG 1 cut(s) 976
EcoT22I ATGCAT 1 cut(s) 1232
ErhI CCWWGG 1 cut(s) 976
FaeI CATG 7 cut(s) 49, 267, 980, 988, 1153, 1230, 1234
FalI AAGNNNNNCTT 2 cut(s) 593, 625
FatI CATG 7 cut(s) 45, 263, 976, 984, 1149, 1226, 1230
FbaI TGATCA 2 cut(s) 1065, 1155
FblI GTMKAC 1 cut(s) 750
Fnu4HI GCNGC 6 cut(s) 375, 542, 839, 959, 993, 1189
FokI GGATG 4 cut(s) 449, 690, 936, 959
Fsp4HI GCNGC 6 cut(s) 375, 542, 839, 959, 993, 1189
FspBI CTAG 1 cut(s) 605
GlaI GCGC 3 cut(s) 57, 495, 1192
GluI GCNGC 6 cut(s) 375, 542, 839, 959, 993, 1189
GsaI CCCAGC 1 cut(s) 255
GsuI CTGGAG 3 cut(s) 611, 803, 1073
HaeIII GGCC 2 cut(s) 282, 515
HgaI GACGC 2 cut(s) 280, 793
HhaI GCGC 3 cut(s) 58, 496, 1193
Hin1I GRCGYC 1 cut(s) 804
Hin1II CATG 7 cut(s) 49, 267, 980, 988, 1153, 1230, 1234
Hin6I GCGC 3 cut(s) 56, 494, 1191
HinP1I GCGC 3 cut(s) 56, 494, 1191
HincII GTYRAC 3 cut(s) 270, 501, 925
HindII GTYRAC 3 cut(s) 270, 501, 925
HphI GGTGA 3 cut(s) 1071, 1075, 1165
Hpy166II GTNNAC 5 cut(s) 270, 501, 751, 925, 1224
Hpy188I TCNGA 6 cut(s) 204, 528, 699, 1005, 1017, 1170
Hpy8I GTNNAC 5 cut(s) 270, 501, 751, 925, 1224
HpyAV CCTTC 4 cut(s) 91, 360, 548, 868
HpyCH4III ACNGT 4 cut(s) 505, 537, 1013, 1072
HpyF10VI GCNNNNNNNGC 5 cut(s) 257, 345, 601, 737, 989
HpyF3I CTNAG 2 cut(s) 290, 429
Hsp92I GRCGYC 1 cut(s) 804
Hsp92II CATG 7 cut(s) 49, 267, 980, 988, 1153, 1230, 1234
HspAI GCGC 3 cut(s) 56, 494, 1191
Ksp22I TGATCA 2 cut(s) 1065, 1155
Kzo9I GATC 4 cut(s) 487, 1065, 1076, 1155
LmnI GCTCC 3 cut(s) 120, 592, 980
Lsp1109I GCAGC 6 cut(s) 361, 528, 825, 945, 1004, 1200
LweI GCATC 1 cut(s) 7
MaeI CTAG 1 cut(s) 605
MaeIII GTNAC 2 cut(s) 164, 1178
MalI GATC 4 cut(s) 489, 1067, 1078, 1157
MboI GATC 4 cut(s) 487, 1065, 1076, 1155
MboII GAAGA 9 cut(s) 121, 202, 433, 712, 896, 1018, 1147, 1157, 1186
MfeI CAATTG 3 cut(s) 573, 625, 726
MflI RGATCY 1 cut(s) 487
MhlI GDGCHC 1 cut(s) 1109
MmeI TCCRAC 2 cut(s) 462, 665
MnlI CCTC 8 cut(s) 295, 325, 478, 652, 661, 708, 875, 913
Mph1103I ATGCAT 1 cut(s) 1232
MseI TTAA 4 cut(s) 368, 395, 413, 942
MspR9I CCNGG 1 cut(s) 136
MunI CAATTG 3 cut(s) 573, 625, 726
Mva1269I GAATGC 1 cut(s) 1093
MvaI CCWGG 1 cut(s) 136
MvnI CGCG 1 cut(s) 58
MwoI GCNNNNNNNGC 5 cut(s) 257, 345, 601, 737, 989
NcoI CCATGG 1 cut(s) 976
NdeII GATC 4 cut(s) 487, 1065, 1076, 1155
NlaIII CATG 7 cut(s) 49, 267, 980, 988, 1153, 1230, 1234
NlaIV GGNNCC 4 cut(s) 122, 283, 489, 982
NmuCI GTSAC 1 cut(s) 164
NsiI ATGCAT 1 cut(s) 1232
NspI RCATGY 1 cut(s) 1230
NspV TTCGAA 2 cut(s) 307, 353
PceI AGGCCT 1 cut(s) 515
PctI GAATGC 1 cut(s) 1093
PfeI GAWTC 4 cut(s) 688, 718, 1028, 1138
PflMI CCANNNNNTGG 1 cut(s) 93
PkrI GCNGC 6 cut(s) 376, 543, 840, 960, 994, 1190
PpuMI RGGWCCY 1 cut(s) 661
Psp5II RGGWCCY 1 cut(s) 661
Psp6I CCWGG 1 cut(s) 134
PspFI CCCAGC 1 cut(s) 251
PspGI CCWGG 1 cut(s) 134
PspN4I GGNNCC 4 cut(s) 122, 283, 489, 982
PspPI GGNCC 2 cut(s) 281, 661
PspPPI RGGWCCY 1 cut(s) 661
PstI CTGCAG 1 cut(s) 843
PsuI RGATCY 1 cut(s) 487
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
SaqAI TTAA 4 cut(s) 368, 395, 413, 942
SatI GCNGC 6 cut(s) 375, 542, 839, 959, 993, 1189
Sau3AI GATC 4 cut(s) 487, 1065, 1076, 1155
Sau96I GGNCC 2 cut(s) 281, 661
ScrFI CCNGG 1 cut(s) 136
SduI GDGCHC 1 cut(s) 1109
SfaNI GCATC 1 cut(s) 7
SfcI CTRYAG 1 cut(s) 839
SfuI TTCGAA 2 cut(s) 307, 353
SinI GGWCC 1 cut(s) 661
SmlI CTYRAG 6 cut(s) 299, 452, 665, 712, 1112, 1142
SmoI CTYRAG 6 cut(s) 299, 452, 665, 712, 1112, 1142
SseBI AGGCCT 1 cut(s) 515
SspMI CTAG 1 cut(s) 605
StuI AGGCCT 1 cut(s) 515
StyD4I CCNGG 1 cut(s) 134
StyI CCWWGG 1 cut(s) 976
TaaI ACNGT 4 cut(s) 505, 537, 1013, 1072
TaqI TCGA 2 cut(s) 307, 353
TfiI GAWTC 4 cut(s) 688, 718, 1028, 1138
Tru1I TTAA 4 cut(s) 368, 395, 413, 942
Tru9I TTAA 4 cut(s) 368, 395, 413, 942
TscAI CASTG 6 cut(s) 169, 602, 848, 1065, 1077, 1089
TseFI GTSAC 1 cut(s) 164
TseI GCWGC 6 cut(s) 374, 541, 838, 958, 992, 1188
Tsp45I GTSAC 1 cut(s) 164
TspDTI ATGAA 7 cut(s) 62, 252, 357, 656, 887, 1187, 1247
TspGWI ACGGA 1 cut(s) 133
TspRI CASTG 6 cut(s) 169, 602, 848, 1065, 1077, 1089
Van91I CCANNNNNTGG 1 cut(s) 93
VpaK11BI GGWCC 1 cut(s) 661
XagI CCTNNNNNAGG 1 cut(s) 289
XapI RAATTY 2 cut(s) 881, 964
XceI RCATGY 1 cut(s) 1230
XmiI GTMKAC 1 cut(s) 750
XspI CTAG 1 cut(s) 605
Zsp2I ATGCAT 1 cut(s) 1232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.