MD15G1422700.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010481
Physical Location & Seq
Forward (+)
52440909 .. 52443421
2513 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1422700.v1.1.491

Sequence Viewer

Length: 1359 bp
ATGGTTTGTTGTGAAGCACCAGAGACGTTGAGAGAGAGCAAGCGGAAATTGGCAAACGCTCTCAGATCCGTTGCAAATTTCAATTCAAATTTCCATCTAATGGACCCTCTGCCTGATGCTGTTGTTCAATACATATTGTCGTATATGGAAAACGCCAAAGACGTTGCAATTTGTAATTGTGTATCCAAGCGATGGAAGGACTCAATGCCTTATATAAGGAGCCTTTACTTCCCCAGAAGCTCCTTTGATAGCCATATGGGTGGAGACAGTCCTGATGACATTGTATTGAAGATGGTTTCAATGATTGAACGATTAGAAAATCTTGTTGTTTATAGTCCCTTCTCGGGTGCTGGCCTTGCTTCTTGGTTATTGATCATAGGTCCCTCTCTTAAGTATCTGGAGCTTCGAATGGACAATATTGCTGAATATCAGACCTCTCTTGAAAGCCCTTCAAAACTGGACTACTTAAGTGCTGCAGAAAATTTGGAGTCTTTAAAACTTTGGGGAGTTTTAGTGGCCCGTTCCCCAAAGTGGGATGTCTTCCAGAAACTCAGGAACCTTGAAATTGTTGGAGCGAAACTGGAGGATCCTGCATTGTCAACTGTGCTTCAGGCCTGTCCGAATCTTACCAATTTGGTGTTACTTGGTTGTGAAGGAGTGAGATCGGTTTCAATTGAGTTGCCATATTTGGAGCAGTGTAAGCTGGACTTTTATGGCTTGGGCAACTGCTCATTTTCCATGGGGTGCCCAAAAATTAAATTCCTTGAGGTGCAAGGCTGTAGCTGGATTCGGGTTCGTGACACAAAGCTTTTGAAAAATCTTTCTATTGCCAATAATGCAGGGAGAGTCTACATGGTTGATTTTGAGAAACTTGTCGCTCTAGAGTTCTTATCAATTAGGGGAGTCCAGTGGTGTTGGGACGCAATAAGAAATATGCTTCAATGTGCAAGTGAAGTGAAACACCTTTTCATGAAGGTGGAATTCACAGGGGATTCTGAGATCCTTCAACCCTTTCCAGAGGTTGACTTCGTTGATTTCTTCAATAACCATCCCAAGCTGCAAAAGTTTGATATCCATGGAGCCATGTTTGCTGCCCTTTGTCAGAAAAACAGCCTGAAAAATGTTGATTCAGGATTTTCAATTCCTTGCCTGGAAGAAGTGATGATCACAGTGAGATCACCGCTGAATGCTGAACAGAAAATGAGCACTCTTGAGTCCTTGTTGAAGTACGCGAAGAATCTGAAGACAATGGTGATAAAGATTCTTCAGATGAAGAGCAGTCATAGCAGTGCAGATGATTTCTTCGACGAGATTTGCAGGTTTAGACACATGAACCGAAAGATCGTTAGAATAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0009506 GO:0030054 GO:0055044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

453

Amino Acids

51.38

Weight (kDa)

6.68

Isoelectric Point (pI)

48.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 147 - 233 9.3e-07 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010481)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10780 AT1G10780
fragaria_vesca FvH4_5g29550 FvH4_5g29550
malus_domestica MD08G1228700.v1.1 MD15G1422700.v1.1
prunus_persica Prupe.1G568000_v2.0.a1
pyrus_communis pycom15g37460
rosa_chinensis RchiOBHm_Chr7g0222501
rosa_laevigata RLG00000002049
rosa_multiflora Rmu_ssc0000146.1_g000020
rosa_roxburghii Rroxscaffold_3G00236730
rosa_rugosa Rorug07G0209900 Rorug07G0210000 Rorug07G0210000
rosa_samantha Rh7AG351700 Rh7BG342300 Rh7CG369100 Rh7DG348400
rosa_wichuraiana Rw7G029690 Rw7G030020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1308
AccB1I GGYRCC 1 cut(s) 744
AccB7I CCANNNNNTGG 2 cut(s) 100, 192
AccI GTMKAC 1 cut(s) 849
AccII CGCG 1 cut(s) 1232
AciI CCGC 2 cut(s) 43, 1181
AclWI GGATC 4 cut(s) 60, 581, 594, 994
AcsI RAATTY 5 cut(s) 76, 88, 481, 758, 980
AcuI CTGAAG 3 cut(s) 593, 1250, 1262
AfaI GTAC 1 cut(s) 1229
AfiI CCNNNNNNNGG 5 cut(s) 100, 192, 344, 531, 532
AflII CTTAAG 2 cut(s) 389, 466
AjnI CCWGG 1 cut(s) 1149
AluBI AGCT 6 cut(s) 240, 403, 703, 783, 808, 1057
AluI AGCT 6 cut(s) 240, 403, 703, 783, 808, 1057
Alw21I GWGCWC 1 cut(s) 1208
Alw26I GTCTC 2 cut(s) 17, 258
AlwI GGATC 4 cut(s) 60, 581, 594, 994
Ama87I CYCGRG 1 cut(s) 343
AoxI GGCC 3 cut(s) 352, 516, 612
ApeKI GCWGC 3 cut(s) 473, 1057, 1091
ApoI RAATTY 5 cut(s) 76, 88, 481, 758, 980
AspS9I GGNCC 3 cut(s) 103, 380, 517
AsuHPI GGTGA 2 cut(s) 1170, 1264
AsuII TTCGAA 1 cut(s) 406
AvaI CYCGRG 1 cut(s) 343
AvaII GGWCC 2 cut(s) 103, 380
BaeGI GKGCMC 1 cut(s) 749
BamHI GGATCC 1 cut(s) 586
BanI GGYRCC 1 cut(s) 744
BbsI GAAGAC 2 cut(s) 532, 1250
Bbv12I GWGCWC 1 cut(s) 1208
BbvI GCAGC 3 cut(s) 460, 1044, 1078
BccI CCATC 4 cut(s) 102, 186, 286, 1056
BciT130I CCWGG 1 cut(s) 1151
BciVI GTATCC 1 cut(s) 193
BclI TGATCA 2 cut(s) 372, 1164
BcoDI GTCTC 2 cut(s) 17, 258
BfaI CTAG 1 cut(s) 881
BfmI CTRYAG 2 cut(s) 474, 778
BfrI CTTAAG 2 cut(s) 389, 466
BfuAI ACCTGC 1 cut(s) 1308
BfuI GTATCC 1 cut(s) 193
BisI GCNGC 3 cut(s) 474, 1058, 1092
BlsI GCNGC 3 cut(s) 475, 1059, 1093
Bme1390I CCNGG 1 cut(s) 1151
Bme18I GGWCC 2 cut(s) 103, 380
BmeT110I CYCGRG 1 cut(s) 343
BmgT120I GGNCC 3 cut(s) 103, 380, 517
BmiI GGNNCC 7 cut(s) 105, 221, 382, 557, 588, 746, 1081
BmrFI CCNGG 1 cut(s) 1151
BmsI GCATC 1 cut(s) 106
BpiI GAAGAC 2 cut(s) 532, 1250
BpmI CTGGAG 2 cut(s) 419, 602
Bpu14I TTCGAA 1 cut(s) 406
BpuEI CTTGAG 2 cut(s) 785, 1232
BsaJI CCNNGG 2 cut(s) 738, 1075
BsaXI ACNNNNNCTCC 2 cut(s) 498, 528
Bsc4I CCNNNNNNNGG 5 cut(s) 100, 192, 344, 531, 532
Bse1I ACTGG 3 cut(s) 462, 585, 907
BseBI CCWGG 1 cut(s) 1151
BseDI CCNNGG 2 cut(s) 738, 1075
BseGI GGATG 2 cut(s) 541, 1048
BseLI CCNNNNNNNGG 5 cut(s) 100, 192, 344, 531, 532
BseMII CTCAG 3 cut(s) 76, 565, 987
BseNI ACTGG 3 cut(s) 462, 585, 907
BseSI GKGCMC 1 cut(s) 749
BseXI GCAGC 3 cut(s) 460, 1044, 1078
BsgI GTGCAG 1 cut(s) 1311
Bsh1236I CGCG 1 cut(s) 1232
BshFI GGCC 3 cut(s) 354, 518, 614
BshNI GGYRCC 1 cut(s) 744
BsiHKAI GWGCWC 1 cut(s) 1208
BsiHKCI CYCGRG 1 cut(s) 343
BslFI GGGAC 3 cut(s) 321, 366, 932
BslI CCNNNNNNNGG 5 cut(s) 100, 192, 344, 531, 532
BsmAI GTCTC 2 cut(s) 17, 258
BsmBI CGTCTC 1 cut(s) 17
BsmFI GGGAC 3 cut(s) 321, 366, 932
BsmI GAATGC 1 cut(s) 1192
BsnI GGCC 3 cut(s) 354, 518, 614
BsoBI CYCGRG 1 cut(s) 343
Bsp119I TTCGAA 1 cut(s) 406
Bsp1286I GDGCHC 2 cut(s) 749, 1208
Bsp143I GATC 8 cut(s) 65, 372, 586, 662, 999, 1164, 1175, 1341
Bsp19I CCATGG 2 cut(s) 738, 1075
BspACI CCGC 2 cut(s) 43, 1181
BspANI GGCC 3 cut(s) 354, 518, 614
BspCNI CTCAG 3 cut(s) 75, 564, 988
BspFNI CGCG 1 cut(s) 1232
BspHI TCATGA 1 cut(s) 969
BspLI GGNNCC 7 cut(s) 105, 221, 382, 557, 588, 746, 1081
BspMAI CTGCAG 1 cut(s) 478
BspMI ACCTGC 1 cut(s) 1308
BspPI GGATC 4 cut(s) 60, 581, 594, 994
BspQI GCTCTTC 1 cut(s) 1268
BspT104I TTCGAA 1 cut(s) 406
BspT107I GGYRCC 1 cut(s) 744
BspTI CTTAAG 2 cut(s) 389, 466
BsrI ACTGG 3 cut(s) 462, 585, 907
BssECI CCNNGG 2 cut(s) 738, 1075
BssMI GATC 8 cut(s) 65, 372, 586, 662, 999, 1164, 1175, 1341
BssT1I CCWWGG 2 cut(s) 738, 1075
Bst2UI CCWGG 1 cut(s) 1151
Bst4CI ACNGT 3 cut(s) 269, 604, 1171
Bst6I CTCTTC 1 cut(s) 1268
BstAFI CTTAAG 2 cut(s) 389, 466
BstBI TTCGAA 1 cut(s) 406
BstC8I GCNNGC 2 cut(s) 41, 352
BstDEI CTNAG 3 cut(s) 62, 551, 996
BstDSI CCRYGG 2 cut(s) 738, 1075
BstF5I GGATG 2 cut(s) 541, 1048
BstFNI CGCG 1 cut(s) 1232
BstKTI GATC 8 cut(s) 68, 375, 589, 665, 1002, 1167, 1178, 1344
BstMAI GTCTC 2 cut(s) 17, 258
BstMBI GATC 8 cut(s) 65, 372, 586, 662, 999, 1164, 1175, 1341
BstMWI GCNNNNNNNGC 5 cut(s) 356, 700, 836, 1088, 1284
BstNI CCWGG 1 cut(s) 1151
BstSCI CCNGG 1 cut(s) 1149
BstSFI CTRYAG 2 cut(s) 474, 778
BstSLI GKGCMC 1 cut(s) 749
BstUI CGCG 1 cut(s) 1232
BstV1I GCAGC 3 cut(s) 460, 1044, 1078
BstV2I GAAGAC 2 cut(s) 532, 1250
BstX2I RGATCY 3 cut(s) 65, 586, 999
BstXI CCANNNNNNTGG 1 cut(s) 260
BstYI RGATCY 3 cut(s) 65, 586, 999
BsuI GTATCC 1 cut(s) 193
BsuRI GGCC 3 cut(s) 354, 518, 614
BtgI CCRYGG 2 cut(s) 738, 1075
BtgZI GCGATG 1 cut(s) 205
BtsCI GGATG 2 cut(s) 541, 1048
BtsI GCAGTG 2 cut(s) 701, 1294
BtsIMutI CAGTG 4 cut(s) 701, 914, 1176, 1294
BveI ACCTGC 1 cut(s) 1308
Cac8I GCNNGC 2 cut(s) 41, 352
CciI TCATGA 1 cut(s) 969
Cfr13I GGNCC 3 cut(s) 103, 380, 517
CseI GACGC 1 cut(s) 929
Csp6I GTAC 1 cut(s) 1228
CviAII CATG 6 cut(s) 739, 853, 970, 1076, 1084, 1330
CviQI GTAC 1 cut(s) 1228
DdeI CTNAG 3 cut(s) 62, 551, 996
DpnI GATC 8 cut(s) 67, 374, 588, 664, 1001, 1166, 1177, 1343
DpnII GATC 8 cut(s) 65, 372, 586, 662, 999, 1164, 1175, 1341
DraI TTTAAA 1 cut(s) 495
Eam1104I CTCTTC 1 cut(s) 1268
EarI CTCTTC 1 cut(s) 1268
Eco130I CCWWGG 2 cut(s) 738, 1075
Eco147I AGGCCT 1 cut(s) 614
Eco32I GATATC 1 cut(s) 1072
Eco47I GGWCC 2 cut(s) 103, 380
Eco57I CTGAAG 3 cut(s) 593, 1250, 1262
Eco88I CYCGRG 1 cut(s) 343
EcoO109I RGGNCCY 1 cut(s) 380
EcoRI GAATTC 1 cut(s) 980
EcoRII CCWGG 1 cut(s) 1149
EcoRV GATATC 1 cut(s) 1072
EcoT14I CCWWGG 2 cut(s) 738, 1075
ErhI CCWWGG 2 cut(s) 738, 1075
Esp3I CGTCTC 1 cut(s) 17
FaeI CATG 6 cut(s) 742, 856, 973, 1079, 1087, 1333
FalI AAGNNNNNCTT 2 cut(s) 692, 724
FaqI GGGAC 3 cut(s) 321, 366, 932
FatI CATG 6 cut(s) 738, 852, 969, 1075, 1083, 1329
FauNDI CATATG 1 cut(s) 255
FbaI TGATCA 2 cut(s) 372, 1164
FblI GTMKAC 1 cut(s) 849
Fnu4HI GCNGC 3 cut(s) 474, 1058, 1092
FokI GGATG 2 cut(s) 548, 1035
Fsp4HI GCNGC 3 cut(s) 474, 1058, 1092
FspBI CTAG 1 cut(s) 881
GluI GCNGC 3 cut(s) 474, 1058, 1092
GsuI CTGGAG 2 cut(s) 419, 602
HaeIII GGCC 3 cut(s) 354, 518, 614
HgaI GACGC 1 cut(s) 929
Hin1II CATG 6 cut(s) 742, 856, 973, 1079, 1087, 1333
HincII GTYRAC 2 cut(s) 600, 1024
HindII GTYRAC 2 cut(s) 600, 1024
HindIII AAGCTT 1 cut(s) 806
HphI GGTGA 2 cut(s) 1170, 1264
Hpy166II GTNNAC 3 cut(s) 600, 850, 1024
Hpy188I TCNGA 7 cut(s) 65, 432, 621, 997, 1104, 1242, 1269
Hpy8I GTNNAC 3 cut(s) 600, 850, 1024
Hpy99I CGWCG 1 cut(s) 1310
HpyAV CCTTC 6 cut(s) 190, 349, 459, 647, 967, 1013
HpyCH4III ACNGT 3 cut(s) 269, 604, 1171
HpyCH4IV ACGT 2 cut(s) 26, 162
HpyF10VI GCNNNNNNNGC 5 cut(s) 356, 700, 836, 1088, 1284
HpyF3I CTNAG 3 cut(s) 62, 551, 996
HpySE526I ACGT 2 cut(s) 26, 162
Hsp92II CATG 6 cut(s) 742, 856, 973, 1079, 1087, 1333
Ksp22I TGATCA 2 cut(s) 372, 1164
Kzo9I GATC 8 cut(s) 65, 372, 586, 662, 999, 1164, 1175, 1341
LguI GCTCTTC 1 cut(s) 1268
LmnI GCTCC 6 cut(s) 219, 245, 400, 572, 691, 1079
Lsp1109I GCAGC 3 cut(s) 460, 1044, 1078
LweI GCATC 1 cut(s) 106
MaeI CTAG 1 cut(s) 881
MaeII ACGT 2 cut(s) 26, 162
MaeIII GTNAC 2 cut(s) 639, 797
MalI GATC 8 cut(s) 67, 374, 588, 664, 1001, 1166, 1177, 1343
MboI GATC 8 cut(s) 65, 372, 586, 662, 999, 1164, 1175, 1341
MboII GAAGA 9 cut(s) 301, 532, 1030, 1166, 1246, 1255, 1256, 1285, 1294
MfeI CAATTG 1 cut(s) 672
MflI RGATCY 3 cut(s) 65, 586, 999
MhlI GDGCHC 2 cut(s) 749, 1208
MlyI GAGTC 5 cut(s) 194, 497, 855, 912, 1223
MmeI TCCRAC 1 cut(s) 550
MnlI CCTC 6 cut(s) 117, 394, 445, 577, 760, 1012
MseI TTAA 4 cut(s) 390, 467, 494, 756
MslI CAYNNNNRTG 3 cut(s) 258, 974, 1287
MspA1I CMGCKG 1 cut(s) 1183
MspCI CTTAAG 2 cut(s) 389, 466
MspR9I CCNGG 1 cut(s) 1151
MunI CAATTG 1 cut(s) 672
Mva1269I GAATGC 1 cut(s) 1192
MvaI CCWGG 1 cut(s) 1151
MvnI CGCG 1 cut(s) 1232
MwoI GCNNNNNNNGC 5 cut(s) 356, 700, 836, 1088, 1284
NcoI CCATGG 2 cut(s) 738, 1075
NdeI CATATG 1 cut(s) 255
NdeII GATC 8 cut(s) 65, 372, 586, 662, 999, 1164, 1175, 1341
NlaIII CATG 6 cut(s) 742, 856, 973, 1079, 1087, 1333
NlaIV GGNNCC 7 cut(s) 105, 221, 382, 557, 588, 746, 1081
NmuCI GTSAC 1 cut(s) 797
NspV TTCGAA 1 cut(s) 406
PagI TCATGA 1 cut(s) 969
PceI AGGCCT 1 cut(s) 614
PciSI GCTCTTC 1 cut(s) 1268
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PctI GAATGC 1 cut(s) 1192
PfeI GAWTC 6 cut(s) 622, 787, 992, 1127, 1237, 1261
PflMI CCANNNNNTGG 2 cut(s) 100, 192
PkrI GCNGC 3 cut(s) 475, 1059, 1093
PleI GAGTC 5 cut(s) 194, 496, 854, 911, 1222
PpsI GAGTC 5 cut(s) 194, 496, 854, 911, 1222
PpuMI RGGWCCY 1 cut(s) 380
Psp5II RGGWCCY 1 cut(s) 380
Psp6I CCWGG 1 cut(s) 1149
PspGI CCWGG 1 cut(s) 1149
PspN4I GGNNCC 7 cut(s) 105, 221, 382, 557, 588, 746, 1081
PspPI GGNCC 3 cut(s) 103, 380, 517
PspPPI RGGWCCY 1 cut(s) 380
PstI CTGCAG 1 cut(s) 478
PsuI RGATCY 3 cut(s) 65, 586, 999
RsaI GTAC 1 cut(s) 1229
RsaNI GTAC 1 cut(s) 1228
RseI CAYNNNNRTG 3 cut(s) 258, 974, 1287
SapI GCTCTTC 1 cut(s) 1268
SaqAI TTAA 4 cut(s) 390, 467, 494, 756
SatI GCNGC 3 cut(s) 474, 1058, 1092
Sau3AI GATC 8 cut(s) 65, 372, 586, 662, 999, 1164, 1175, 1341
Sau96I GGNCC 3 cut(s) 103, 380, 517
SchI GAGTC 5 cut(s) 194, 497, 855, 912, 1223
ScrFI CCNGG 1 cut(s) 1151
SduI GDGCHC 2 cut(s) 749, 1208
SfaNI GCATC 1 cut(s) 106
SfcI CTRYAG 2 cut(s) 474, 778
SfuI TTCGAA 1 cut(s) 406
SinI GGWCC 2 cut(s) 103, 380
SmiMI CAYNNNNRTG 3 cut(s) 258, 974, 1287
SmlI CTYRAG 4 cut(s) 389, 466, 764, 1211
SmoI CTYRAG 4 cut(s) 389, 466, 764, 1211
SseBI AGGCCT 1 cut(s) 614
SsiI CCGC 2 cut(s) 43, 1181
SspI AATATT 1 cut(s) 418
SspMI CTAG 1 cut(s) 881
StuI AGGCCT 1 cut(s) 614
StyD4I CCNGG 1 cut(s) 1149
StyI CCWWGG 2 cut(s) 738, 1075
TaaI ACNGT 3 cut(s) 269, 604, 1171
TaiI ACGT 2 cut(s) 29, 165
TaqI TCGA 2 cut(s) 406, 1305
TfiI GAWTC 6 cut(s) 622, 787, 992, 1127, 1237, 1261
Tru1I TTAA 4 cut(s) 390, 467, 494, 756
Tru9I TTAA 4 cut(s) 390, 467, 494, 756
TscAI CASTG 4 cut(s) 701, 914, 1176, 1294
TseFI GTSAC 1 cut(s) 797
TseI GCWGC 3 cut(s) 473, 1057, 1091
Tsp45I GTSAC 1 cut(s) 797
TspDTI ATGAA 4 cut(s) 958, 986, 1286, 1346
TspGWI ACGGA 1 cut(s) 58
TspRI CASTG 4 cut(s) 701, 914, 1176, 1294
Van91I CCANNNNNTGG 2 cut(s) 100, 192
Vha464I CTTAAG 2 cut(s) 389, 466
VpaK11BI GGWCC 2 cut(s) 103, 380
XapI RAATTY 5 cut(s) 76, 88, 481, 758, 980
XbaI TCTAGA 1 cut(s) 880
XmiI GTMKAC 1 cut(s) 849
XspI CTAG 1 cut(s) 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.