MD08G1233100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010190
Physical Location & Seq
Reverse (-)
29968324 .. 29971956
3633 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1233100.v1.1.491

Sequence Viewer

Length: 885 bp
ATGAAGAAGAGGACAGCGTACGCTTGGGGAGTGGCCATTCTTTGCTTTGTGGTCTTGATGATCGTCACTCCTGCCATTCCCCAGTCTCAGGCTTACCACGATTTTGCTGACCAACGCGAGTTTTTCGGTATTCCAAATACACTTAACGTGGTTTCAAATTTTCCTTTTCTTGTTATTGGTCTCATCGGTCTTTTCCTCTGCTATTACAAGGATTATTTCAAGTTCAGCTTGCAAGGAGAGCTTTGGGGTTGGACATGCTTTTACATCGGCGTAGCTGCTGTTGCAATTGGATCATCATACTATCACCTGAAGCCAAATGATGCCCGTCTTGTGTGGGACCGCTTGCCAATGACAATTGCATTCACATCGATCATTGCAATCTTTATCATTGAAAGGATTGATGAGAGGAAGGGAACAATTTCCATCATACCTCTACTTTTGGCGGGTATAATCAGCATATTGTATTGGAGGTTCTTTGATGACCTCCGTCCATATGCTATGGTCCAGTTTCTTCCTTGCATTGCCATTCCTCTGATGGCTATCTTGTTACCCCCAATGTACACTCATTCCACATATTGGCTTTGGGCAGCAGGATTTTATCTTTTAGCTAAGGTGGAAGAAGCTGCGGATAAAGTGATCTACGATTGGACTCATCATATTGTCAGCGGGCACACCCTTAAGCATTTGTGCGCTGCAATGGTTCCTGTATTCTTGACACTTATGCTTGCAAAGAGGACTGTTGAACCAGAGAGGCAAAGTTTGCTCAAGGTTTGGAAGATTTCCTGGACTAATTTTAGGAAGAAAGATGGCACAGTGGAGAGTGATGGCACTGTGGAGAGTTACACTTGTACCTACTCAACAGTTCCGGTTGAGGAATCACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ceramidase

Protein Analysis

295

Amino Acids

33.73

Weight (kDa)

8.13

Isoelectric Point (pI)

35.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase PF05875 47 - 162 3.5e-08 Ceramidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010190)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71190 AT1G71190
fragaria_vesca FvH4_5g29250 FvH4_5g29250 FvH4_5g29250 FvH4_5g29250 FvH4_5g29250
malus_domestica MD08G1233100.v1.1
prunus_persica Prupe.1G571000_v2.0.a1
pyrus_communis pycom08g20200
rosa_chinensis RchiOBHm_Chr7g0222011
rosa_laevigata RLG00000002103
rosa_multiflora Rmu_sc0004325.1_g000004
rosa_roxburghii Rroxscaffold_3G00237500
rosa_rugosa Rorug07G0204900 Rorug07G0205000
rosa_samantha Rh7AG345500 Rh7BG336000 Rh7CG363200 Rh7DG343800
rosa_wichuraiana Rw7G029300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 576
AccII CGCG 1 cut(s) 117
AciI CCGC 4 cut(s) 340, 443, 626, 666
AclWI GGATC 1 cut(s) 298
AcoI YGGCCR 1 cut(s) 33
AcsI RAATTY 1 cut(s) 157
AcuI CTGAAG 1 cut(s) 329
AfaI GTAC 3 cut(s) 20, 560, 850
AfiI CCNNNNNNNGG 2 cut(s) 88, 576
AflII CTTAAG 1 cut(s) 677
AgsI TTSAA 4 cut(s) 156, 220, 392, 743
AjnI CCWGG 1 cut(s) 782
AluBI AGCT 5 cut(s) 228, 241, 275, 608, 623
AluI AGCT 5 cut(s) 228, 241, 275, 608, 623
Alw26I GTCTC 2 cut(s) 90, 185
AlwI GGATC 1 cut(s) 298
AoxI GGCC 1 cut(s) 33
ApeKI GCWGC 4 cut(s) 275, 587, 623, 692
ApoI RAATTY 1 cut(s) 157
Asp700I GAANNNNTTC 1 cut(s) 418
AspLEI GCGC 1 cut(s) 692
AspS9I GGNCC 2 cut(s) 337, 502
AsuHPI GGTGA 1 cut(s) 296
AvaII GGWCC 2 cut(s) 337, 502
BaeGI GKGCMC 1 cut(s) 672
BaeI ACNNNNGTAYC 2 cut(s) 832, 865
BalI TGGCCA 1 cut(s) 35
BbvI GCAGC 4 cut(s) 262, 599, 610, 679
BccI CCATC 4 cut(s) 431, 529, 800, 818
BcgI CGANNNNNNTGC 2 cut(s) 348, 382
BciT130I CCWGG 1 cut(s) 784
BcoDI GTCTC 2 cut(s) 90, 185
BfrI CTTAAG 1 cut(s) 677
BisI GCNGC 4 cut(s) 276, 588, 624, 693
BlsI GCNGC 4 cut(s) 277, 589, 625, 694
Bme1390I CCNGG 1 cut(s) 784
Bme18I GGWCC 2 cut(s) 337, 502
BmgT120I GGNCC 2 cut(s) 337, 502
BmiI GGNNCC 2 cut(s) 338, 702
BmrFI CCNGG 1 cut(s) 784
BmrI ACTGGG 1 cut(s) 76
BmsI GCATC 1 cut(s) 310
BmuI ACTGGG 1 cut(s) 76
BoxI GACNNNNGTC 1 cut(s) 486
Bpu10I CCTNAGC 1 cut(s) 609
BpuEI CTTGAG 1 cut(s) 749
Bsa29I ATCGAT 1 cut(s) 368
BsaBI GATNNNNATC 1 cut(s) 539
BsaI GGTCTC 1 cut(s) 185
BsaWI WCCGGW 1 cut(s) 865
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 576
Bse1I ACTGG 2 cut(s) 82, 505
Bse3DI GCAATG 3 cut(s) 372, 519, 702
Bse8I GATNNNNATC 1 cut(s) 539
BseBI CCWGG 1 cut(s) 784
BseCI ATCGAT 1 cut(s) 368
BseJI GATNNNNATC 1 cut(s) 539
BseLI CCNNNNNNNGG 2 cut(s) 88, 576
BseMI GCAATG 3 cut(s) 372, 519, 702
BseMII CTCAG 1 cut(s) 101
BseNI ACTGG 2 cut(s) 82, 505
BseSI GKGCMC 1 cut(s) 672
BseXI GCAGC 4 cut(s) 262, 599, 610, 679
Bsh1236I CGCG 1 cut(s) 117
BshFI GGCC 1 cut(s) 35
BshVI ATCGAT 1 cut(s) 368
BsiSI CCGG 1 cut(s) 866
BsiWI CGTACG 1 cut(s) 18
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 2 cut(s) 88, 576
BsmAI GTCTC 2 cut(s) 90, 185
BsmFI GGGAC 1 cut(s) 350
BsmI GAATGC 1 cut(s) 359
BsnI GGCC 1 cut(s) 35
Bso31I GGTCTC 1 cut(s) 185
Bsp1286I GDGCHC 1 cut(s) 672
Bsp1407I TGTACA 1 cut(s) 558
Bsp143I GATC 4 cut(s) 60, 290, 369, 636
BspACI CCGC 4 cut(s) 340, 443, 626, 666
BspANI GGCC 1 cut(s) 35
BspCNI CTCAG 1 cut(s) 100
BspDI ATCGAT 1 cut(s) 368
BspFNI CGCG 1 cut(s) 117
BspLI GGNNCC 2 cut(s) 338, 702
BspPI GGATC 1 cut(s) 298
BspTI CTTAAG 1 cut(s) 677
BspTNI GGTCTC 1 cut(s) 185
BsrDI GCAATG 3 cut(s) 372, 519, 702
BsrGI TGTACA 1 cut(s) 558
BsrI ACTGG 2 cut(s) 82, 505
BssMI GATC 4 cut(s) 60, 290, 369, 636
Bst2UI CCWGG 1 cut(s) 784
Bst4CI ACNGT 4 cut(s) 739, 814, 832, 862
Bst6I CTCTTC 1 cut(s) 2
BstAFI CTTAAG 1 cut(s) 677
BstAPI GCANNNNNTGC 1 cut(s) 760
BstAUI TGTACA 1 cut(s) 558
BstC8I GCNNGC 4 cut(s) 230, 344, 668, 726
BstDEI CTNAG 2 cut(s) 87, 609
BstFNI CGCG 1 cut(s) 117
BstHHI GCGC 1 cut(s) 692
BstKTI GATC 4 cut(s) 63, 293, 372, 639
BstMAI GTCTC 2 cut(s) 90, 185
BstMBI GATC 4 cut(s) 60, 290, 369, 636
BstMWI GCNNNNNNNGC 3 cut(s) 238, 281, 760
BstNI CCWGG 1 cut(s) 784
BstNSI RCATGY 1 cut(s) 258
BstPAI GACNNNNGTC 1 cut(s) 486
BstSCI CCNGG 1 cut(s) 782
BstSLI GKGCMC 1 cut(s) 672
BstUI CGCG 1 cut(s) 117
BstV1I GCAGC 4 cut(s) 262, 599, 610, 679
Bsu15I ATCGAT 1 cut(s) 368
BsuRI GGCC 1 cut(s) 35
BsuTUI ATCGAT 1 cut(s) 368
BtsIMutI CAGTG 2 cut(s) 819, 828
Cac8I GCNNGC 4 cut(s) 230, 344, 668, 726
CfoI GCGC 1 cut(s) 692
Cfr13I GGNCC 2 cut(s) 337, 502
ClaI ATCGAT 1 cut(s) 368
Csp6I GTAC 3 cut(s) 19, 559, 849
CviAII CATG 1 cut(s) 255
CviQI GTAC 3 cut(s) 19, 559, 849
DdeI CTNAG 2 cut(s) 87, 609
DpnI GATC 4 cut(s) 62, 292, 371, 638
DpnII GATC 4 cut(s) 60, 290, 369, 636
EaeI YGGCCR 1 cut(s) 33
Eam1104I CTCTTC 1 cut(s) 2
EarI CTCTTC 1 cut(s) 2
Eco31I GGTCTC 1 cut(s) 185
Eco47I GGWCC 2 cut(s) 337, 502
Eco57I CTGAAG 1 cut(s) 329
EcoRII CCWGG 1 cut(s) 782
FaeI CATG 1 cut(s) 258
FalI AAGNNNNNCTT 4 cut(s) 212, 244, 225, 257
FaqI GGGAC 1 cut(s) 350
FatI CATG 1 cut(s) 254
FauI CCCGC 2 cut(s) 436, 659
FauNDI CATATG 1 cut(s) 493
Fnu4HI GCNGC 4 cut(s) 276, 588, 624, 693
Fsp4HI GCNGC 4 cut(s) 276, 588, 624, 693
GlaI GCGC 1 cut(s) 691
GluI GCNGC 4 cut(s) 276, 588, 624, 693
HaeIII GGCC 1 cut(s) 35
HapII CCGG 1 cut(s) 866
HhaI GCGC 1 cut(s) 692
Hin1II CATG 1 cut(s) 258
Hin6I GCGC 1 cut(s) 690
HinP1I GCGC 1 cut(s) 690
HinfI GANTC 2 cut(s) 649, 875
HpaII CCGG 1 cut(s) 866
HphI GGTGA 1 cut(s) 296
Hpy166II GTNNAC 1 cut(s) 561
Hpy188I TCNGA 1 cut(s) 534
Hpy188III TCNNGA 3 cut(s) 55, 712, 879
Hpy8I GTNNAC 1 cut(s) 561
HpyAV CCTTC 1 cut(s) 403
HpyCH4III ACNGT 4 cut(s) 739, 814, 832, 862
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 7 cut(s) 232, 284, 359, 377, 519, 695, 728
HpyF10VI GCNNNNNNNGC 3 cut(s) 238, 281, 760
HpyF3I CTNAG 2 cut(s) 87, 609
HpySE526I ACGT 1 cut(s) 147
Hsp92II CATG 1 cut(s) 258
HspAI GCGC 1 cut(s) 690
Kzo9I GATC 4 cut(s) 60, 290, 369, 636
Lsp1109I GCAGC 4 cut(s) 262, 599, 610, 679
LweI GCATC 1 cut(s) 310
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 3 cut(s) 64, 546, 839
MalI GATC 4 cut(s) 62, 292, 371, 638
MboI GATC 4 cut(s) 60, 290, 369, 636
MboII GAAGA 6 cut(s) 16, 19, 503, 629, 787, 811
MfeI CAATTG 2 cut(s) 285, 354
MhlI GDGCHC 1 cut(s) 672
MlsI TGGCCA 1 cut(s) 35
MluCI AATT 5 cut(s) 157, 285, 354, 417, 790
MluNI TGGCCA 1 cut(s) 35
MlyI GAGTC 1 cut(s) 643
MmeI TCCRAC 1 cut(s) 230
Mox20I TGGCCA 1 cut(s) 35
MroXI GAANNNNTTC 1 cut(s) 418
MscI TGGCCA 1 cut(s) 35
MseI TTAA 2 cut(s) 144, 678
Msp20I TGGCCA 1 cut(s) 35
MspA1I CMGCKG 1 cut(s) 666
MspCI CTTAAG 1 cut(s) 677
MspI CCGG 1 cut(s) 866
MspR9I CCNGG 1 cut(s) 784
MunI CAATTG 2 cut(s) 285, 354
Mva1269I GAATGC 1 cut(s) 359
MvaI CCWGG 1 cut(s) 784
MvnI CGCG 1 cut(s) 117
MwoI GCNNNNNNNGC 3 cut(s) 238, 281, 760
NdeI CATATG 1 cut(s) 493
NdeII GATC 4 cut(s) 60, 290, 369, 636
NlaIII CATG 1 cut(s) 258
NlaIV GGNNCC 2 cut(s) 338, 702
NmuCI GTSAC 1 cut(s) 64
NspI RCATGY 1 cut(s) 258
PctI GAATGC 1 cut(s) 359
PdmI GAANNNNTTC 1 cut(s) 418
PfeI GAWTC 1 cut(s) 875
Pfl23II CGTACG 1 cut(s) 18
PflMI CCANNNNNTGG 1 cut(s) 576
PfoI TCCNGGA 1 cut(s) 782
PkrI GCNGC 4 cut(s) 277, 589, 625, 694
PleI GAGTC 1 cut(s) 643
PpsI GAGTC 1 cut(s) 643
PshAI GACNNNNGTC 1 cut(s) 486
Psp6I CCWGG 1 cut(s) 782
PspGI CCWGG 1 cut(s) 782
PspLI CGTACG 1 cut(s) 18
PspN4I GGNNCC 2 cut(s) 338, 702
PspPI GGNCC 2 cut(s) 337, 502
PsrI GAACNNNNNNTAC 2 cut(s) 455, 487
RsaI GTAC 3 cut(s) 20, 560, 850
RsaNI GTAC 3 cut(s) 19, 559, 849
SaqAI TTAA 2 cut(s) 144, 678
SatI GCNGC 4 cut(s) 276, 588, 624, 693
Sau3AI GATC 4 cut(s) 60, 290, 369, 636
Sau96I GGNCC 2 cut(s) 337, 502
SchI GAGTC 1 cut(s) 643
ScrFI CCNGG 1 cut(s) 784
SduI GDGCHC 1 cut(s) 672
SfaNI GCATC 1 cut(s) 310
SinI GGWCC 2 cut(s) 337, 502
SmlI CTYRAG 2 cut(s) 677, 764
SmoI CTYRAG 2 cut(s) 677, 764
Sse9I AATT 5 cut(s) 157, 285, 354, 417, 790
SsiI CCGC 4 cut(s) 340, 443, 626, 666
StyD4I CCNGG 1 cut(s) 782
TaaI ACNGT 4 cut(s) 739, 814, 832, 862
TaiI ACGT 1 cut(s) 150
TaqI TCGA 1 cut(s) 368
TaqII GACCGA 1 cut(s) 176
TasI AATT 5 cut(s) 157, 285, 354, 417, 790
TatI WGTACW 1 cut(s) 558
TfiI GAWTC 1 cut(s) 875
Tru1I TTAA 2 cut(s) 144, 678
Tru9I TTAA 2 cut(s) 144, 678
TscAI CASTG 2 cut(s) 819, 835
TseFI GTSAC 1 cut(s) 64
TseI GCWGC 4 cut(s) 275, 587, 623, 692
Tsp45I GTSAC 1 cut(s) 64
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 476
TspRI CASTG 2 cut(s) 819, 835
Van91I CCANNNNNTGG 1 cut(s) 576
Vha464I CTTAAG 1 cut(s) 677
VpaK11BI GGWCC 2 cut(s) 337, 502
XapI RAATTY 1 cut(s) 157
XceI RCATGY 1 cut(s) 258
XcmI CCANNNNNNNNNTGG 1 cut(s) 532
XmnI GAANNNNTTC 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.