Rroxscaffold_3G00237500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0010190
Physical Location & Seq
Reverse (-)
25148311 .. 25151597
3287 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00237500.1

Sequence Viewer

Length: 957 bp
ATGAAGAAGAGAACGATGTACGCCTGGGGACTGGCCCTCCTCTGCTTTGTGGTCCTGATGATCGTCACTCCTGCCATCCCTCAGTCTCAAGCTTACCATGATTTCGCTGATCAGCGCGAGTTTTTCGGCATTCCCAATACCCTTAATGTGATTTCAAATTTCCCTTTCCTGGTCGTTGGTATCATTGGTCTTGTCCTTTGTTATTATGGGAACTATATGAAGCTCAGCTTGCAAGGTGAGCTTTGGGGTTGGACATGTTTCTACCTTGGTGTGGCTGCTGTTGGGATTGGATCCTCATATTATCATCTTAACCCAAATGACGCTACTCTAGTGTGGGATCGCTTGCCTATGACAATTGCATTCACATCAATCATTGCAATTTTCATCATTGAAAGGATTGATGAGCGGAAGGGAACCATTTCCATTTTACCTCTGCTTTTGGCGGGTGTAATAAGCATATTGTATTGGAGGCAAGCACTGTACCTCATTCCTTTTTATTATAATGGCCACTCTTTCCTAAGCTGTATTTACACTGTAGAGGAACTAGTTGTAATCATGAAATTTTTTGATGACCTCCGCCCATATGCTCTGATCCAGTTTCTTCCTTGCATTGCCATTCCCCTGATGGCTATCTTGTTGCCCCCTATGTACACACATTCCACATATTGGCTTTGGGCAGCAGTGTTTTATCTTTTAGCCAAGGTGGAAGAAGCTGGTGATAAAGTGATCTACAAATGGACATATCACATTGTTAGTGGGCACACCCTTAAGCATCTGTGCGCCGCAATGGTTCCTGTCTTCTTGACGCTTATGCTTGCAAAGAGAACTATTGAAACAGAGAGGCAAAGTTTGCTCAAGATTTGGAAGGTTTCCTGGACCGAGTATAGAAAGAAGGGTAATGACACGGTGGTAGAGAGTTACACTTATACTACTGTTCCGGTTGTGGAATCACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ceramidase

Protein Analysis

318

Amino Acids

36.57

Weight (kDa)

8.11

Isoelectric Point (pI)

37.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase PF05875 47 - 181 7.1e-09 Ceramidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010190)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71190 AT1G71190
fragaria_vesca FvH4_5g29250 FvH4_5g29250 FvH4_5g29250 FvH4_5g29250 FvH4_5g29250
malus_domestica MD08G1233100.v1.1
prunus_persica Prupe.1G571000_v2.0.a1
pyrus_communis pycom08g20200
rosa_chinensis RchiOBHm_Chr7g0222011
rosa_laevigata RLG00000002103
rosa_multiflora Rmu_sc0004325.1_g000004
rosa_roxburghii Rroxscaffold_3G00237500
rosa_rugosa Rorug07G0204900 Rorug07G0205000
rosa_samantha Rh7AG345500 Rh7BG336000 Rh7CG363200 Rh7DG343800
rosa_wichuraiana Rw7G029300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 501
AccB7I CCANNNNNTGG 1 cut(s) 666
AccBSI CCGCTC 1 cut(s) 406
AccII CGCG 1 cut(s) 117
AciI CCGC 4 cut(s) 406, 443, 577, 783
AclWI GGATC 4 cut(s) 285, 298, 345, 586
AcoI YGGCCR 1 cut(s) 505
AcsI RAATTY 2 cut(s) 157, 560
AfaI GTAC 3 cut(s) 20, 482, 650
AfiI CCNNNNNNNGG 3 cut(s) 169, 271, 666
AflII CTTAAG 1 cut(s) 767
AflIII ACRYGT 1 cut(s) 254
AgsI TTSAA 3 cut(s) 156, 392, 833
AhlI ACTAGT 1 cut(s) 544
AjnI CCWGG 3 cut(s) 23, 168, 872
AluBI AGCT 6 cut(s) 92, 223, 228, 241, 522, 713
AluI AGCT 6 cut(s) 92, 223, 228, 241, 522, 713
Alw26I GTCTC 1 cut(s) 90
AlwI GGATC 4 cut(s) 285, 298, 345, 586
AoxI GGCC 2 cut(s) 33, 505
ApeKI GCWGC 2 cut(s) 275, 677
ApoI RAATTY 2 cut(s) 157, 560
Asp700I GAANNNNTTC 1 cut(s) 418
AspLEI GCGC 2 cut(s) 117, 782
AspS9I GGNCC 3 cut(s) 34, 52, 876
AsuHPI GGTGA 2 cut(s) 248, 728
AvaII GGWCC 2 cut(s) 52, 876
BaeGI GKGCMC 1 cut(s) 762
BalI TGGCCA 1 cut(s) 507
BamHI GGATCC 1 cut(s) 290
BbsI GAAGAC 1 cut(s) 790
BbvI GCAGC 2 cut(s) 262, 689
BccI CCATC 2 cut(s) 83, 619
BciT130I CCWGG 3 cut(s) 25, 170, 874
BclI TGATCA 1 cut(s) 109
BcoDI GTCTC 1 cut(s) 90
BcuI ACTAGT 1 cut(s) 544
BfaI CTAG 3 cut(s) 329, 545, 955
BfmI CTRYAG 1 cut(s) 534
BfrI CTTAAG 1 cut(s) 767
BisI GCNGC 3 cut(s) 276, 678, 783
BlpI GCTNAGC 1 cut(s) 224
BlsI GCNGC 3 cut(s) 277, 679, 784
Bme1390I CCNGG 3 cut(s) 25, 170, 874
Bme18I GGWCC 2 cut(s) 52, 876
BmgT120I GGNCC 3 cut(s) 34, 52, 876
BmiI GGNNCC 3 cut(s) 292, 415, 792
BmrFI CCNGG 3 cut(s) 25, 170, 874
BmsI GCATC 1 cut(s) 781
BpiI GAAGAC 1 cut(s) 790
Bpu10I CCTNAGC 1 cut(s) 518
Bpu1102I GCTNAGC 1 cut(s) 224
BpuEI CTTGAG 2 cut(s) 72, 839
BsaBI GATNNNNATC 1 cut(s) 629
BsaJI CCNNGG 3 cut(s) 24, 265, 699
BsaWI WCCGGW 1 cut(s) 937
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bsc4I CCNNNNNNNGG 3 cut(s) 169, 271, 666
Bse1I ACTGG 2 cut(s) 36, 595
Bse3DI GCAATG 3 cut(s) 372, 609, 792
Bse8I GATNNNNATC 1 cut(s) 629
BseBI CCWGG 3 cut(s) 25, 170, 874
BseDI CCNNGG 3 cut(s) 24, 265, 699
BseGI GGATG 1 cut(s) 75
BseJI GATNNNNATC 1 cut(s) 629
BseLI CCNNNNNNNGG 3 cut(s) 169, 271, 666
BseMI GCAATG 3 cut(s) 372, 609, 792
BseMII CTCAG 2 cut(s) 95, 238
BseNI ACTGG 2 cut(s) 36, 595
BseRI GAGGAG 1 cut(s) 29
BseSI GKGCMC 1 cut(s) 762
BseXI GCAGC 2 cut(s) 262, 689
Bsh1236I CGCG 1 cut(s) 117
BshFI GGCC 2 cut(s) 35, 507
BsiSI CCGG 1 cut(s) 938
BslFI GGGAC 1 cut(s) 42
BslI CCNNNNNNNGG 3 cut(s) 169, 271, 666
BsmAI GTCTC 1 cut(s) 90
BsmFI GGGAC 1 cut(s) 42
BsmI GAATGC 2 cut(s) 129, 359
BsnI GGCC 2 cut(s) 35, 507
Bsp1286I GDGCHC 1 cut(s) 762
Bsp1407I TGTACA 1 cut(s) 648
Bsp143I GATC 6 cut(s) 60, 109, 290, 337, 591, 726
Bsp1720I GCTNAGC 1 cut(s) 224
BspACI CCGC 4 cut(s) 406, 443, 577, 783
BspANI GGCC 2 cut(s) 35, 507
BspCNI CTCAG 2 cut(s) 94, 237
BspFNI CGCG 1 cut(s) 117
BspHI TCATGA 1 cut(s) 555
BspLI GGNNCC 3 cut(s) 292, 415, 792
BspPI GGATC 4 cut(s) 285, 298, 345, 586
BspTI CTTAAG 1 cut(s) 767
BsrBI CCGCTC 1 cut(s) 406
BsrDI GCAATG 3 cut(s) 372, 609, 792
BsrGI TGTACA 1 cut(s) 648
BsrI ACTGG 2 cut(s) 36, 595
BssECI CCNNGG 3 cut(s) 24, 265, 699
BssMI GATC 6 cut(s) 60, 109, 290, 337, 591, 726
BssT1I CCWWGG 2 cut(s) 265, 699
Bst2UI CCWGG 3 cut(s) 25, 170, 874
Bst4CI ACNGT 4 cut(s) 480, 535, 907, 934
Bst6I CTCTTC 1 cut(s) 2
BstAFI CTTAAG 1 cut(s) 767
BstAPI GCANNNNNTGC 1 cut(s) 850
BstAUI TGTACA 1 cut(s) 648
BstC8I GCNNGC 4 cut(s) 230, 344, 474, 816
BstDEI CTNAG 3 cut(s) 81, 224, 518
BstF5I GGATG 1 cut(s) 75
BstFNI CGCG 1 cut(s) 117
BstHHI GCGC 2 cut(s) 117, 782
BstKTI GATC 6 cut(s) 63, 112, 293, 340, 594, 729
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 6 cut(s) 60, 109, 290, 337, 591, 726
BstMWI GCNNNNNNNGC 3 cut(s) 229, 238, 850
BstNI CCWGG 3 cut(s) 25, 170, 874
BstNSI RCATGY 1 cut(s) 258
BstSCI CCNGG 3 cut(s) 23, 168, 872
BstSFI CTRYAG 1 cut(s) 534
BstSLI GKGCMC 1 cut(s) 762
BstUI CGCG 1 cut(s) 117
BstV1I GCAGC 2 cut(s) 262, 689
BstV2I GAAGAC 1 cut(s) 790
BstX2I RGATCY 1 cut(s) 290
BstYI RGATCY 1 cut(s) 290
BsuRI GGCC 2 cut(s) 35, 507
BtsCI GGATG 1 cut(s) 75
BtsI GCAGTG 1 cut(s) 687
BtsIMutI CAGTG 3 cut(s) 476, 531, 687
Cac8I GCNNGC 4 cut(s) 230, 344, 474, 816
CciI TCATGA 1 cut(s) 555
CfoI GCGC 2 cut(s) 117, 782
Cfr13I GGNCC 3 cut(s) 34, 52, 876
CseI GACGC 2 cut(s) 329, 814
Csp6I GTAC 3 cut(s) 19, 481, 649
CviAII CATG 3 cut(s) 98, 255, 556
CviQI GTAC 3 cut(s) 19, 481, 649
DdeI CTNAG 3 cut(s) 81, 224, 518
DpnI GATC 6 cut(s) 62, 111, 292, 339, 593, 728
DpnII GATC 6 cut(s) 60, 109, 290, 337, 591, 726
EaeI YGGCCR 1 cut(s) 505
Eam1104I CTCTTC 1 cut(s) 2
EarI CTCTTC 1 cut(s) 2
EciI GGCGGA 1 cut(s) 566
Eco130I CCWWGG 2 cut(s) 265, 699
Eco47I GGWCC 2 cut(s) 52, 876
EcoRII CCWGG 3 cut(s) 23, 168, 872
EcoT14I CCWWGG 2 cut(s) 265, 699
ErhI CCWWGG 2 cut(s) 265, 699
FaeI CATG 3 cut(s) 101, 258, 559
FalI AAGNNNNNCTT 4 cut(s) 212, 244, 225, 257
FaqI GGGAC 1 cut(s) 42
FatI CATG 3 cut(s) 97, 254, 555
FauI CCCGC 1 cut(s) 436
FauNDI CATATG 1 cut(s) 583
FbaI TGATCA 1 cut(s) 109
Fnu4HI GCNGC 3 cut(s) 276, 678, 783
FokI GGATG 1 cut(s) 62
Fsp4HI GCNGC 3 cut(s) 276, 678, 783
FspBI CTAG 3 cut(s) 329, 545, 955
GlaI GCGC 2 cut(s) 116, 781
GluI GCNGC 3 cut(s) 276, 678, 783
HaeIII GGCC 2 cut(s) 35, 507
HapII CCGG 1 cut(s) 938
HgaI GACGC 2 cut(s) 329, 814
HhaI GCGC 2 cut(s) 117, 782
Hin1II CATG 3 cut(s) 101, 258, 559
Hin6I GCGC 2 cut(s) 115, 780
HinP1I GCGC 2 cut(s) 115, 780
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 1 cut(s) 947
HpaII CCGG 1 cut(s) 938
HphI GGTGA 2 cut(s) 248, 728
Hpy166II GTNNAC 1 cut(s) 651
Hpy188I TCNGA 1 cut(s) 591
Hpy188III TCNNGA 4 cut(s) 55, 556, 802, 856
Hpy8I GTNNAC 1 cut(s) 651
HpyAV CCTTC 3 cut(s) 403, 859, 886
HpyCH4III ACNGT 4 cut(s) 480, 535, 907, 934
HpyCH4V TGCA 5 cut(s) 232, 359, 377, 609, 818
HpyF10VI GCNNNNNNNGC 3 cut(s) 229, 238, 850
HpyF3I CTNAG 3 cut(s) 81, 224, 518
Hsp92II CATG 3 cut(s) 101, 258, 559
HspAI GCGC 2 cut(s) 115, 780
Ksp22I TGATCA 1 cut(s) 109
Kzo9I GATC 6 cut(s) 60, 109, 290, 337, 591, 726
Lsp1109I GCAGC 2 cut(s) 262, 689
LweI GCATC 1 cut(s) 781
MaeI CTAG 3 cut(s) 329, 545, 955
MaeIII GTNAC 2 cut(s) 64, 917
MalI GATC 6 cut(s) 62, 111, 292, 339, 593, 728
MbiI CCGCTC 1 cut(s) 406
MboI GATC 6 cut(s) 60, 109, 290, 337, 591, 726
MboII GAAGA 5 cut(s) 16, 19, 593, 719, 790
MfeI CAATTG 1 cut(s) 354
MflI RGATCY 1 cut(s) 290
MhlI GDGCHC 1 cut(s) 762
MlsI TGGCCA 1 cut(s) 507
MluCI AATT 4 cut(s) 157, 354, 378, 560
MluNI TGGCCA 1 cut(s) 507
MmeI TCCRAC 1 cut(s) 230
Mox20I TGGCCA 1 cut(s) 507
MroXI GAANNNNTTC 1 cut(s) 418
MscI TGGCCA 1 cut(s) 507
MseI TTAA 3 cut(s) 144, 309, 768
Msp20I TGGCCA 1 cut(s) 507
MspCI CTTAAG 1 cut(s) 767
MspI CCGG 1 cut(s) 938
MspR9I CCNGG 3 cut(s) 25, 170, 874
MunI CAATTG 1 cut(s) 354
Mva1269I GAATGC 2 cut(s) 129, 359
MvaI CCWGG 3 cut(s) 25, 170, 874
MvnI CGCG 1 cut(s) 117
MwoI GCNNNNNNNGC 3 cut(s) 229, 238, 850
NdeI CATATG 1 cut(s) 583
NdeII GATC 6 cut(s) 60, 109, 290, 337, 591, 726
NlaIII CATG 3 cut(s) 101, 258, 559
NlaIV GGNNCC 3 cut(s) 292, 415, 792
NmuCI GTSAC 1 cut(s) 64
NspI RCATGY 1 cut(s) 258
PagI TCATGA 1 cut(s) 555
PciI ACATGT 1 cut(s) 254
PctI GAATGC 2 cut(s) 129, 359
PdmI GAANNNNTTC 1 cut(s) 418
PfeI GAWTC 1 cut(s) 947
PflMI CCANNNNNTGG 1 cut(s) 666
PfoI TCCNGGA 1 cut(s) 872
PkrI GCNGC 3 cut(s) 277, 679, 784
PscI ACATGT 1 cut(s) 254
PsiI TTATAA 1 cut(s) 501
Psp6I CCWGG 3 cut(s) 23, 168, 872
PspGI CCWGG 3 cut(s) 23, 168, 872
PspN4I GGNNCC 3 cut(s) 292, 415, 792
PspPI GGNCC 3 cut(s) 34, 52, 876
PsuI RGATCY 1 cut(s) 290
RsaI GTAC 3 cut(s) 20, 482, 650
RsaNI GTAC 3 cut(s) 19, 481, 649
SaqAI TTAA 3 cut(s) 144, 309, 768
SatI GCNGC 3 cut(s) 276, 678, 783
Sau3AI GATC 6 cut(s) 60, 109, 290, 337, 591, 726
Sau96I GGNCC 3 cut(s) 34, 52, 876
ScrFI CCNGG 3 cut(s) 25, 170, 874
SduI GDGCHC 1 cut(s) 762
SfaNI GCATC 1 cut(s) 781
SfcI CTRYAG 1 cut(s) 534
SinI GGWCC 2 cut(s) 52, 876
SmlI CTYRAG 3 cut(s) 87, 767, 854
SmoI CTYRAG 3 cut(s) 87, 767, 854
SpeI ACTAGT 1 cut(s) 544
Sse9I AATT 4 cut(s) 157, 354, 378, 560
SsiI CCGC 4 cut(s) 406, 443, 577, 783
SspMI CTAG 3 cut(s) 329, 545, 955
StyD4I CCNGG 3 cut(s) 23, 168, 872
StyI CCWWGG 2 cut(s) 265, 699
TaaI ACNGT 4 cut(s) 480, 535, 907, 934
TaqII GACCGA 1 cut(s) 893
TasI AATT 4 cut(s) 157, 354, 378, 560
TatI WGTACW 1 cut(s) 648
TauI GCSGC 1 cut(s) 785
TfiI GAWTC 1 cut(s) 947
Tru1I TTAA 3 cut(s) 144, 309, 768
Tru9I TTAA 3 cut(s) 144, 309, 768
TscAI CASTG 3 cut(s) 483, 538, 687
TseFI GTSAC 1 cut(s) 64
TseI GCWGC 2 cut(s) 275, 677
Tsp45I GTSAC 1 cut(s) 64
TspDTI ATGAA 4 cut(s) 17, 233, 373, 572
TspRI CASTG 3 cut(s) 483, 538, 687
Van91I CCANNNNNTGG 1 cut(s) 666
Vha464I CTTAAG 1 cut(s) 767
VpaK11BI GGWCC 2 cut(s) 52, 876
XapI RAATTY 2 cut(s) 157, 560
XceI RCATGY 1 cut(s) 258
XcmI CCANNNNNNNNNTGG 1 cut(s) 622
XmnI GAANNNNTTC 1 cut(s) 418
XspI CTAG 3 cut(s) 329, 545, 955
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.