Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0009218

Physical Location & Seq

Genomic Coordinates Forward (+)

30311524 .. 30315859

Gene Structure 4336 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1235700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2025 bp

ATGATGAAACTTGCATGTGGTTTTTGGTTGTTTCTCTCTCTACTTTGTCTCTCTACCTTATCTCCATCAACCGCAGCAGAAGCACCAGCACCAGCACCAGCTCCTCCTCCCCCCACAACCAAAGTAACAACAACAGCAACATGTCCCATGGATCTGAGCTATGTCCTAAGAATCCCCTTCAACTCCTCCACCTGCAAAAACTTCCAACCTCCTCCCAAAACCCCACAAATGGAGTCGGAGGACCCCACCAATAACCCCTGCTGCCAAACCCTTTTGTCCCTCTTCGGAATCTCCTTCGCGCAACGCCTCAAAGAAACCTCCCTCTTCAACCTCCCCAACCTCCCCACCTCCATTTCCTGCCTCCAAAACTACCAGTCCAAGCTCTCCTCCCTCTCCTCCCTCTCCCTCCCTCCCAATATTGTCAACTACTGCTGGGACCCTCTCCAGTTTGTCATCACCCCCAACATCTGCGCCAACATTCAGTCCACCAATGATTGGGTCTCCAAGCTCGGCCACTCCACCGTCCTTGACTCCGCCTGCCGCCCTGACCTCACCGATCTCTCCTCCTGCGGGGGCTGCGTCGACGCCGGCCTTGCCGTTCAGAAGAAGCTGCTTGCCGTTGATGGTAACTCTTCTCACACTCGGGACTGTTGGTACTTTACTATTCTTTATGCTGCTGGTATTGTTAACGAGTTTGGCCCTGAGAGTAATGGTGCTGTGAACTGTATATTTGGGTTGTCGTTTGATTCAAATACGCCTTCGTCGAAAAAGAGCAATACTGCTCTTGTTTTCGGCCTCACTGGCGCTGGAGTTGCGTTGTTTGTTATGTGTAGTTTGTTGGGATTGTACTTTTGGTATGATAGGAGGTGGAAGAACGAAGGGATGGAATCCGGTTTCGACTTGGAAGAACGAGATTCTAGGAGTAGAGTGAGACCAAACACTGGTTCAATTTGGTTCAAAATTCAGGACCTTGAGAAGGCAACTAATAACTTTTCGCAGAAGAATTTTATCGGTCGAGGTGGGTTTGGTGTTGTTTACAAGGGTGTGTTGCAGGATGGGACTACAGTTGCTGTTAAGAAAGTTATAGAATCTGATATTCAAGGGGATGCTGAGTTTTGCAATGAGGTTGAGATTATTAGCAATTTGAGGCACCGAAACCTTGTCCCGCTTAGAGGGTGTTGTATGGTCGAGGGGGAAGACAATTACGAGGAGAAAGGAAGTCACAGGTACCTTGTCTACGATTACATGCCGAATGGGAATCTAGATGACCATCTCTTTATTTCTCAGTCAAGTAATCCGAGCGGAATTGAAAGGAGACCCTTGACTTGGCCTCAAAGAAAGAGCATAATATTGGATGTGGCCAAGGGTTTAGCTTATCTGCACTATGGAGTGAAGCCTGCAATATATCACAGAGATATTAAGGCGACAAACATACTACTAGATGCAGATATGAAAGCGAGGGTGGCGGACTTTGGGCTTGCAAAACAGAGCATGGAAGGCCAGTCCCATCTCACTACTAGAGTGGCAGGGACTCACGGGTACTTAGCCCCCGAATACGCTCTTTATGGACAGCTGACTGAGAAGAGCGATGTTTATAGCTTTGGAGTGGTTATTTTGGAGATTATGTGTGGGAGGAAAGCTCTTGATTTGTCTTTGGGATCCCCTCGTGCTTTTCTGATCACAGATTGGGCTTGGTCATTGGTGAAATCTGGAAAAGCTGAGCAGGCTTTAGACTTTTCATTGGTGAAAGATGGGGATAATGCGAATTCGAATCCGAAGAGCATAATGGAGAGATATTTGCTGGTCGGCATTTTGTGTGCTCATGTAATGGTGGCTTTACGGCCTACCATTTTGGATGCCCTGAAAATGTTGGAGGGAGATATTGAGGTGCCACGGATTCCAGATAGGCCGATGCCCCTTGGTCATCCTTCAAGTTATGGCAACGACAACGGTAACACGTTCAGCATATCACCAGCTTTGAGCGGCCCAAAATTCCAAAGCGGAGACATGCTCAGGACATGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

675

Amino Acids

73.98

Weight (kDa)

6.17

Isoelectric Point (pI)

45.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPARK	PF19160	44 - 217	7.4e-40	SPARK
Pkinase	PF00069	332 - 549	4.6e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	334 - 545	4e-41	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009218)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11050
fragaria_vesca	FvH4_5g29100 FvH4_5g29100 FvH4_5g29100
malus_domestica	MD08G1235700.v1.1 MD15G1427400.v1.1
prunus_persica	Prupe.1G571200_v2.0.a1 Prupe.1G571200_v2.0.a1
pyrus_communis	pycom08g20370 pycom15g37890
rosa_chinensis	RchiOBHm_Chr7g0221741
rosa_laevigata	RLG00000002134
rosa_multiflora	Rmu_co8451303.1_g000001 Rmu_sc0003311.1_g000001
rosa_roxburghii	Rroxscaffold_3G00238080
rosa_rugosa	Rorug07G0203100
rosa_samantha	Rh7AG344000 Rh7BG334500 Rh7CG361400 Rh7DG341800
rosa_wichuraiana	Rw0G003240 Rw7G029170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	200
Acc36I	ACCTGC	1 cut(s)	200
Acc65I	GGTACC	1 cut(s)	1227
AccB1I	GGYRCC	3 cut(s)	1149, 1227, 1888
AccB7I	CCANNNNNTGG	2 cut(s)	495, 941
AccBSI	CCGCTC	2 cut(s)	1302, 1983
AccI	GTMKAC	2 cut(s)	582, 1236
AccII	CGCG	1 cut(s)	299
AciI	CCGC	9 cut(s)	72, 534, 541, 570, 1166, 1302, 1466, 1983, 2001
AclWI	GGATC	3 cut(s)	159, 1653, 1666
AcoI	YGGCCR	2 cut(s)	511, 1359
AcsI	RAATTY	4 cut(s)	960, 1003, 1765, 1991
AcyI	GRCGYC	1 cut(s)	585
AfaI	GTAC	4 cut(s)	656, 848, 1229, 1541
AfiI	CCNNNNNNNGG	7 cut(s)	229, 495, 570, 941, 976, 1172, 1326
AflIII	ACRYGT	2 cut(s)	140, 1956
AgsI	TTSAA	8 cut(s)	181, 328, 750, 948, 958, 1100, 1310, 1932
AjuI	GAANNNNNNNTTGG	2 cut(s)	928, 960
Alw21I	GWGCWC	1 cut(s)	1822
Alw26I	GTCTC	5 cut(s)	53, 505, 925, 1309, 1998
AlwI	GGATC	3 cut(s)	159, 1653, 1666
AlwNI	CAGNNNCTG	1 cut(s)	1070
Ama87I	CYCGRG	1 cut(s)	642
ApeKI	GCWGC	5 cut(s)	74, 261, 576, 610, 674
ApoI	RAATTY	4 cut(s)	960, 1003, 1765, 1991
Asp718I	GGTACC	1 cut(s)	1227
AspLEI	GCGC	3 cut(s)	301, 473, 806
AspS9I	GGNCC	5 cut(s)	241, 436, 698, 967, 1985
AsuHPI	GGTGA	5 cut(s)	448, 544, 1714, 1756, 1962
AsuII	TTCGAA	1 cut(s)	1769
AvaI	CYCGRG	1 cut(s)	642
AvaII	GGWCC	3 cut(s)	241, 436, 967
BalI	TGGCCA	1 cut(s)	1361
BamHI	GGATCC	1 cut(s)	1658
BanI	GGYRCC	3 cut(s)	1149, 1227, 1888
BauI	CACGAG	1 cut(s)	1665
BbsI	GAAGAC	1 cut(s)	1203
Bbv12I	GWGCWC	1 cut(s)	1822
BbvI	GCAGC	5 cut(s)	86, 248, 563, 597, 661
BccI	CCATC	7 cut(s)	73, 617, 877, 1049, 1278, 1515, 1745
BceAI	ACGGC	3 cut(s)	581, 602, 1856
BclI	TGATCA	1 cut(s)	1677
BcoDI	GTCTC	5 cut(s)	53, 505, 925, 1309, 1998
BfaI	CTAG	4 cut(s)	918, 1262, 1439, 1518
BfmI	CTRYAG	1 cut(s)	1062
BfoI	RGCGCY	1 cut(s)	807
BfuAI	ACCTGC	1 cut(s)	200
BglI	GCCNNNNNGGC	1 cut(s)	801
BisI	GCNGC	7 cut(s)	75, 262, 541, 577, 611, 675, 1984
BlpI	GCTNAGC	1 cut(s)	1719
BlsI	GCNGC	7 cut(s)	76, 263, 542, 578, 612, 676, 1985
Bme18I	GGWCC	3 cut(s)	241, 436, 967
BmeT110I	CYCGRG	1 cut(s)	642
BmgT120I	GGNCC	5 cut(s)	241, 436, 698, 967, 1985
BmiI	GGNNCC	7 cut(s)	243, 437, 438, 1151, 1229, 1660, 1890
BmsI	GCATC	4 cut(s)	1096, 1432, 1846, 1902
BpiI	GAAGAC	1 cut(s)	1203
BplI	GAGNNNNNCTC	3 cut(s)	1624, 1656, 1995
BpmI	CTGGAG	2 cut(s)	428, 828
Bpu10I	CCTNAGC	1 cut(s)	2012
Bpu1102I	GCTNAGC	1 cut(s)	1719
Bpu14I	TTCGAA	1 cut(s)	1769
BpuEI	CTTGAG	1 cut(s)	992
BsaBI	GATNNNNATC	1 cut(s)	1269
BsaHI	GRCGYC	1 cut(s)	585
BsaI	GGTCTC	3 cut(s)	505, 925, 1309
BsaJI	CCNNGG	4 cut(s)	147, 1362, 1892, 1918
BsaWI	WCCGGW	1 cut(s)	890
BsaXI	ACNNNNNCTCC	4 cut(s)	46, 76, 1610, 1640
Bsc4I	CCNNNNNNNGG	7 cut(s)	229, 495, 570, 941, 976, 1172, 1326
Bse118I	RCCGGY	1 cut(s)	587
Bse1I	ACTGG	5 cut(s)	373, 445, 805, 946, 1501
Bse3DI	GCAATG	1 cut(s)	1126
Bse8I	GATNNNNATC	1 cut(s)	1269
BseDI	CCNNGG	4 cut(s)	147, 1362, 1892, 1918
BseGI	GGATG	6 cut(s)	888, 1060, 1111, 1360, 1861, 1924
BseJI	GATNNNNATC	1 cut(s)	1269
BseLI	CCNNNNNNNGG	7 cut(s)	229, 495, 570, 941, 976, 1172, 1326
BseMI	GCAATG	1 cut(s)	1126
BseMII	CTCAG	6 cut(s)	146, 693, 1101, 1298, 1569, 1710
BseNI	ACTGG	5 cut(s)	373, 445, 805, 946, 1501
BseRI	GAGGAG	8 cut(s)	93, 96, 175, 201, 376, 385, 553, 1223
BseXI	GCAGC	5 cut(s)	86, 248, 563, 597, 661
BseYI	CCCAGC	1 cut(s)	432
BsgI	GTGCAG	1 cut(s)	1364
Bsh1236I	CGCG	1 cut(s)	299
Bsh1285I	CGRYCG	1 cut(s)	1015
BshNI	GGYRCC	3 cut(s)	1149, 1227, 1888
BsiEI	CGRYCG	1 cut(s)	1015
BsiHKAI	GWGCWC	1 cut(s)	1822
BsiHKCI	CYCGRG	1 cut(s)	642
BsiSI	CCGG	2 cut(s)	588, 891
BslFI	GGGAC	8 cut(s)	129, 262, 449, 659, 1072, 1148, 1489, 1543
BslI	CCNNNNNNNGG	7 cut(s)	229, 495, 570, 941, 976, 1172, 1326
BsmAI	GTCTC	5 cut(s)	53, 505, 925, 1309, 1998
BsmFI	GGGAC	8 cut(s)	129, 262, 449, 659, 1072, 1148, 1489, 1543
Bso31I	GGTCTC	3 cut(s)	505, 925, 1309
BsoBI	CYCGRG	1 cut(s)	642
Bsp119I	TTCGAA	1 cut(s)	1769
Bsp1286I	GDGCHC	1 cut(s)	1822
Bsp143I	GATC	4 cut(s)	151, 556, 1658, 1677
Bsp1720I	GCTNAGC	1 cut(s)	1719
Bsp19I	CCATGG	1 cut(s)	147
BspACI	CCGC	9 cut(s)	72, 534, 541, 570, 1166, 1302, 1466, 1983, 2001
BspCNI	CTCAG	7 cut(s)	147, 694, 1102, 1297, 1570, 1711, 2025
BspFNI	CGCG	1 cut(s)	299
BspLI	GGNNCC	7 cut(s)	243, 437, 438, 1151, 1229, 1660, 1890
BspMI	ACCTGC	1 cut(s)	200
BspPI	GGATC	3 cut(s)	159, 1653, 1666
BspQI	GCTCTTC	2 cut(s)	1577, 1772
BspT104I	TTCGAA	1 cut(s)	1769
BspT107I	GGYRCC	3 cut(s)	1149, 1227, 1888
BspTNI	GGTCTC	3 cut(s)	505, 925, 1309
BsrBI	CCGCTC	2 cut(s)	1302, 1983
BsrDI	GCAATG	1 cut(s)	1126
BsrFI	RCCGGY	1 cut(s)	587
BsrI	ACTGG	5 cut(s)	373, 445, 805, 946, 1501
BssAI	RCCGGY	1 cut(s)	587
BssECI	CCNNGG	4 cut(s)	147, 1362, 1892, 1918
BssMI	GATC	4 cut(s)	151, 556, 1658, 1677
BssNI	GRCGYC	1 cut(s)	585
BssSI	CACGAG	1 cut(s)	1665
BssT1I	CCWWGG	3 cut(s)	147, 1362, 1918
Bst2BI	CACGAG	1 cut(s)	1665
Bst4CI	ACNGT	5 cut(s)	523, 650, 725, 1066, 1952
Bst6I	CTCTTC	5 cut(s)	287, 329, 637, 1577, 1772
BstACI	GRCGYC	1 cut(s)	585
BstBI	TTCGAA	1 cut(s)	1769
BstC8I	GCNNGC	6 cut(s)	538, 589, 615, 1398, 1479, 1725
BstDSI	CCRYGG	2 cut(s)	147, 1892
BstENI	CCTNNNNNAGG	1 cut(s)	974
BstF5I	GGATG	6 cut(s)	888, 1060, 1111, 1360, 1861, 1924
BstFNI	CGCG	1 cut(s)	299
BstH2I	RGCGCY	1 cut(s)	807
BstHHI	GCGC	3 cut(s)	301, 473, 806
BstKTI	GATC	4 cut(s)	154, 559, 1661, 1680
BstMAI	GTCTC	5 cut(s)	53, 505, 925, 1309, 1998
BstMBI	GATC	4 cut(s)	151, 556, 1658, 1677
BstMCI	CGRYCG	1 cut(s)	1015
BstMWI	GCNNNNNNNGC	8 cut(s)	80, 576, 593, 801, 812, 1463, 1497, 1724
BstNSI	RCATGY	5 cut(s)	18, 144, 1249, 2011, 2022
BstSFI	CTRYAG	1 cut(s)	1062
BstUI	CGCG	1 cut(s)	299
BstV1I	GCAGC	5 cut(s)	86, 248, 563, 597, 661
BstV2I	GAAGAC	1 cut(s)	1203
BstX2I	RGATCY	2 cut(s)	151, 1658
BstYI	RGATCY	2 cut(s)	151, 1658
BtgI	CCRYGG	2 cut(s)	147, 1892
BtgZI	GCGATG	1 cut(s)	1602
BtsCI	GGATG	6 cut(s)	888, 1060, 1111, 1360, 1861, 1924
BtsIMutI	CAGTG	2 cut(s)	798, 939
BveI	ACCTGC	1 cut(s)	200
Cac8I	GCNNGC	6 cut(s)	538, 589, 615, 1398, 1479, 1725
CaiI	CAGNNNCTG	1 cut(s)	1070
CfoI	GCGC	3 cut(s)	301, 473, 806
Cfr10I	RCCGGY	1 cut(s)	587
Cfr13I	GGNCC	5 cut(s)	241, 436, 698, 967, 1985
CseI	GACGC	2 cut(s)	568, 593
Csp6I	GTAC	4 cut(s)	655, 847, 1228, 1540
CspCI	CAANNNNNGTGG	2 cut(s)	508, 543
CviAII	CATG	8 cut(s)	15, 141, 148, 1246, 1492, 1823, 2008, 2019
CviQI	GTAC	4 cut(s)	655, 847, 1228, 1540
DpnI	GATC	4 cut(s)	153, 558, 1660, 1679
DpnII	GATC	4 cut(s)	151, 556, 1658, 1677
EaeI	YGGCCR	2 cut(s)	511, 1359
Eam1104I	CTCTTC	5 cut(s)	287, 329, 637, 1577, 1772
EarI	CTCTTC	5 cut(s)	287, 329, 637, 1577, 1772
EciI	GGCGGA	2 cut(s)	523, 1481
Eco130I	CCWWGG	3 cut(s)	147, 1362, 1918
Eco31I	GGTCTC	3 cut(s)	505, 925, 1309
Eco47I	GGWCC	3 cut(s)	241, 436, 967
Eco88I	CYCGRG	1 cut(s)	642
EcoNI	CCTNNNNNAGG	1 cut(s)	974
EcoO109I	RGGNCCY	3 cut(s)	241, 436, 967
EcoRI	GAATTC	1 cut(s)	1765
EcoT14I	CCWWGG	3 cut(s)	147, 1362, 1918
ErhI	CCWWGG	3 cut(s)	147, 1362, 1918
FaeI	CATG	8 cut(s)	18, 144, 151, 1249, 1495, 1826, 2011, 2022
FaqI	GGGAC	8 cut(s)	129, 262, 449, 659, 1072, 1148, 1489, 1543
FatI	CATG	8 cut(s)	14, 140, 147, 1245, 1491, 1822, 2007, 2018
FauI	CCCGC	2 cut(s)	563, 1173
FbaI	TGATCA	1 cut(s)	1677
FblI	GTMKAC	2 cut(s)	582, 1236
Fnu4HI	GCNGC	7 cut(s)	75, 262, 541, 577, 611, 675, 1984
FokI	GGATG	6 cut(s)	895, 1067, 1118, 1367, 1868, 1911
Fsp4HI	GCNGC	7 cut(s)	75, 262, 541, 577, 611, 675, 1984
FspBI	CTAG	4 cut(s)	918, 1262, 1439, 1518
GlaI	GCGC	3 cut(s)	300, 472, 805
GluI	GCNGC	7 cut(s)	75, 262, 541, 577, 611, 675, 1984
GsaI	CCCAGC	1 cut(s)	436
GsuI	CTGGAG	2 cut(s)	428, 828
HaeII	RGCGCY	1 cut(s)	807
HapII	CCGG	2 cut(s)	588, 891
HgaI	GACGC	2 cut(s)	568, 593
HhaI	GCGC	3 cut(s)	301, 473, 806
Hin1I	GRCGYC	1 cut(s)	585
Hin1II	CATG	8 cut(s)	18, 144, 151, 1249, 1495, 1826, 2011, 2022
Hin6I	GCGC	3 cut(s)	299, 471, 804
HinP1I	GCGC	3 cut(s)	299, 471, 804
HincII	GTYRAC	3 cut(s)	424, 583, 688
HindII	GTYRAC	3 cut(s)	424, 583, 688
HpaI	GTTAAC	1 cut(s)	688
HpaII	CCGG	2 cut(s)	588, 891
HphI	GGTGA	5 cut(s)	448, 544, 1714, 1756, 1962
Hpy166II	GTNNAC	7 cut(s)	424, 486, 583, 688, 721, 1036, 1237
Hpy188I	TCNGA	8 cut(s)	156, 238, 287, 603, 1093, 1299, 1677, 1776
Hpy188III	TCNNGA	7 cut(s)	644, 965, 1262, 1643, 1710, 1901, 2014
Hpy8I	GTNNAC	7 cut(s)	424, 486, 583, 688, 721, 1036, 1237
Hpy99I	CGWCG	3 cut(s)	584, 587, 766
HpyAV	CCTTC	7 cut(s)	187, 304, 768, 872, 970, 1490, 1938
HpyCH4III	ACNGT	5 cut(s)	523, 650, 725, 1066, 1952
HpyCH4IV	ACGT	1 cut(s)	1958
HpyCH4V	TGCA	8 cut(s)	14, 195, 1051, 1119, 1381, 1400, 1445, 1481
HpyF10VI	GCNNNNNNNGC	8 cut(s)	80, 576, 593, 801, 812, 1463, 1497, 1724
HpySE526I	ACGT	1 cut(s)	1958
Hsp92I	GRCGYC	1 cut(s)	585
Hsp92II	CATG	8 cut(s)	18, 144, 151, 1249, 1495, 1826, 2011, 2022
HspAI	GCGC	3 cut(s)	299, 471, 804
KflI	GGGWCCC	1 cut(s)	436
KpnI	GGTACC	1 cut(s)	1231
KroI	GCCGGC	1 cut(s)	587
KroNI	GCCGGC	1 cut(s)	589
Ksp22I	TGATCA	1 cut(s)	1677
KspAI	GTTAAC	1 cut(s)	688
Kzo9I	GATC	4 cut(s)	151, 556, 1658, 1677
LguI	GCTCTTC	2 cut(s)	1577, 1772
LmnI	GCTCC	1 cut(s)	106
Lsp1109I	GCAGC	5 cut(s)	86, 248, 563, 597, 661
LweI	GCATC	4 cut(s)	1096, 1432, 1846, 1902
MaeI	CTAG	4 cut(s)	918, 1262, 1439, 1518
MaeII	ACGT	1 cut(s)	1958
MaeIII	GTNAC	4 cut(s)	124, 626, 1220, 1952
MalI	GATC	4 cut(s)	153, 558, 1660, 1679
MbiI	CCGCTC	2 cut(s)	1302, 1983
MboI	GATC	4 cut(s)	151, 556, 1658, 1677
MflI	RGATCY	2 cut(s)	151, 1658
MhlI	GDGCHC	1 cut(s)	1822
MlsI	TGGCCA	1 cut(s)	1361
MluCI	AATT	8 cut(s)	948, 960, 1003, 1141, 1201, 1305, 1765, 1991
MluNI	TGGCCA	1 cut(s)	1361
MlyI	GAGTC	3 cut(s)	242, 524, 1525
MmeI	TCCRAC	3 cut(s)	216, 229, 1851
Mox20I	TGGCCA	1 cut(s)	1361
MroNI	GCCGGC	1 cut(s)	587
MscI	TGGCCA	1 cut(s)	1361
MseI	TTAA	3 cut(s)	687, 1074, 1419
Msp20I	TGGCCA	1 cut(s)	1361
MspA1I	CMGCKG	1 cut(s)	1573
MspI	CCGG	2 cut(s)	588, 891
MvnI	CGCG	1 cut(s)	299
MwoI	GCNNNNNNNGC	8 cut(s)	80, 576, 593, 801, 812, 1463, 1497, 1724
NaeI	GCCGGC	1 cut(s)	589
NcoI	CCATGG	1 cut(s)	147
NdeII	GATC	4 cut(s)	151, 556, 1658, 1677
NgoMIV	GCCGGC	1 cut(s)	587
NlaIII	CATG	8 cut(s)	18, 144, 151, 1249, 1495, 1826, 2011, 2022
NlaIV	GGNNCC	7 cut(s)	243, 437, 438, 1151, 1229, 1660, 1890
NmeAIII	GCCGAG	1 cut(s)	489
NmuCI	GTSAC	1 cut(s)	1220
NspI	RCATGY	5 cut(s)	18, 144, 1249, 2011, 2022
NspV	TTCGAA	1 cut(s)	1769
PaqCI	CACCTGC	1 cut(s)	200
PciI	ACATGT	1 cut(s)	140
PciSI	GCTCTTC	2 cut(s)	1577, 1772
PcsI	WCGNNNNNNNCGW	1 cut(s)	761
PdiI	GCCGGC	1 cut(s)	589
PfeI	GAWTC	9 cut(s)	171, 288, 746, 887, 914, 1088, 1258, 1771, 1897
PflMI	CCANNNNNTGG	2 cut(s)	495, 941
PkrI	GCNGC	7 cut(s)	76, 263, 542, 578, 612, 676, 1985
PleI	GAGTC	3 cut(s)	241, 524, 1525
PpsI	GAGTC	3 cut(s)	241, 524, 1525
PpuMI	RGGWCCY	3 cut(s)	241, 436, 967
PscI	ACATGT	1 cut(s)	140
Psp5II	RGGWCCY	3 cut(s)	241, 436, 967
PspFI	CCCAGC	1 cut(s)	432
PspN4I	GGNNCC	7 cut(s)	243, 437, 438, 1151, 1229, 1660, 1890
PspPI	GGNCC	5 cut(s)	241, 436, 698, 967, 1985
PspPPI	RGGWCCY	3 cut(s)	241, 436, 967
PstNI	CAGNNNCTG	1 cut(s)	1070
PsuI	RGATCY	2 cut(s)	151, 1658
PvuII	CAGCTG	1 cut(s)	1573
RsaI	GTAC	4 cut(s)	656, 848, 1229, 1541
RsaNI	GTAC	4 cut(s)	655, 847, 1228, 1540
SalI	GTCGAC	1 cut(s)	581
SapI	GCTCTTC	2 cut(s)	1577, 1772
SaqAI	TTAA	3 cut(s)	687, 1074, 1419
SatI	GCNGC	7 cut(s)	75, 262, 541, 577, 611, 675, 1984
Sau3AI	GATC	4 cut(s)	151, 556, 1658, 1677
Sau96I	GGNCC	5 cut(s)	241, 436, 698, 967, 1985
SchI	GAGTC	3 cut(s)	242, 524, 1525
SduI	GDGCHC	1 cut(s)	1822
SfaNI	GCATC	4 cut(s)	1096, 1432, 1846, 1902
SfcI	CTRYAG	1 cut(s)	1062
SfuI	TTCGAA	1 cut(s)	1769
SgrDI	CGTCGACG	1 cut(s)	581
SinI	GGWCC	3 cut(s)	241, 436, 967
SmlI	CTYRAG	1 cut(s)	971
SmoI	CTYRAG	1 cut(s)	971
Sse9I	AATT	8 cut(s)	948, 960, 1003, 1141, 1201, 1305, 1765, 1991
SsiI	CCGC	9 cut(s)	72, 534, 541, 570, 1166, 1302, 1466, 1983, 2001
SspI	AATATT	2 cut(s)	418, 1350
SspMI	CTAG	4 cut(s)	918, 1262, 1439, 1518
StyI	CCWWGG	3 cut(s)	147, 1362, 1918
TaaI	ACNGT	5 cut(s)	523, 650, 725, 1066, 1952
TaiI	ACGT	1 cut(s)	1961
TaqI	TCGA	6 cut(s)	582, 764, 897, 1015, 1188, 1769
TaqII	GACCGA	1 cut(s)	1001
TasI	AATT	8 cut(s)	948, 960, 1003, 1141, 1201, 1305, 1765, 1991
TatI	WGTACW	1 cut(s)	846
TauI	GCSGC	2 cut(s)	543, 1986
TfiI	GAWTC	9 cut(s)	171, 288, 746, 887, 914, 1088, 1258, 1771, 1897
Tru1I	TTAA	3 cut(s)	687, 1074, 1419
Tru9I	TTAA	3 cut(s)	687, 1074, 1419
TscAI	CASTG	2 cut(s)	805, 946
TseFI	GTSAC	1 cut(s)	1220
TseI	GCWGC	5 cut(s)	74, 261, 576, 610, 674
Tsp45I	GTSAC	1 cut(s)	1220
TspDTI	ATGAA	3 cut(s)	20, 1466, 1728
TspGWI	ACGGA	1 cut(s)	1909
TspRI	CASTG	2 cut(s)	805, 946
Van91I	CCANNNNNTGG	2 cut(s)	495, 941
VpaK11BI	GGWCC	3 cut(s)	241, 436, 967
XagI	CCTNNNNNAGG	1 cut(s)	974
XapI	RAATTY	4 cut(s)	960, 1003, 1765, 1991
XbaI	TCTAGA	1 cut(s)	1261
XceI	RCATGY	5 cut(s)	18, 144, 1249, 2011, 2022
XmiI	GTMKAC	2 cut(s)	582, 1236
XspI	CTAG	4 cut(s)	918, 1262, 1439, 1518

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.