Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009218

Physical Location & Seq

Genomic Coordinates Forward (+)

52786566 .. 52789798

Gene Structure 3233 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1427400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2016 bp

ATGATGAAACTTGCAGTTGGGTTTTGGTTGTTTCTCTCTCTACTTTCTCTCTCTACCTTATCTCCATCGACAGCAGCTGAAGCACCAGCACCAGCTCCTCCTCCCAGAACAAATATAACAACAACAGCAGCGTGCCCAATGGATCTGAGCTATGTCCTAAGAATCCCCTTCAACTCCTCCACCTGCAAAAACTTCCAACCTCCTTCCAAAACCACACAAATGGAGTCGGAGGACCTCACCAAGATCCCCTGCTGCCAAACCCTTTTGTCCCTCTTCGGGATCTCCCTCGCCCAACACCTCAAAGAAACCTCCCTCTTCCAACTCCCCAACCTCCCCACCTCCATTTCCTGCCTCCAAAACTTCCAGTCCAAGCTCTCCTCCCTCTCCCTCTCTCCCAATCTTGTCTCCTACTGCTTTGACCCTCTCCAGTTTGTCATCTCCCCCAACATCTGCGCCAACATTCAGTCCACCAGTGATTGGGTCTCCAAGCTCGGCAACTCCACCGTCCTCGACTCCGCCTGCCGCCCTGACCTCGCCGATCTCTCCCTCTGCGGGGGCTGTGTCGACGCCGGCTTTGCCATTCAGAAGATGTTGATTGCCGCCGATGGTAACTCTTCTCACACTAAAGACTGTTGGTACTTTACTATTCTTTATGCTGCTGGTATTGTCAATGAGTTTGGCCCTGAGAGCAATGGTGTTGTGAACTGTATATTTGGGTTGTCTTCTTCCAATGCGCCATCGTCGAAAAAGAGCAATACTGCCCTTGTTTTCGGCCTCACTGGCGCTGGGGTTGCGGTGTTTGTCATGTTTAGTTTGTTGGGGTTGTACTTTTGGTATGATAGGAGGTGGAGGAACAAAAGGATTATAGGGTCTCGAATGGAATCCGGTTTTGACTTGGATGAACGAGATGCTAGGATTAGAGTGAGACCAAACACCGGTTCAATTTGGTTTAAAATTCAGGACCTTGAGAAGGCTACTAACAAATTTTCGCAGAAGAATTTTATCGGTCGAGGTGGATTTGGTGTTGTTTACAAGGGTGTGTTGCAGGACGGGACTACGGTTGCTGTTAAGAAAGTTATAGAATCTGATTTTCAAGGGGATGCTGAATTTTGCAATGAGGTTGAGATTATTAGCAATTTGAAGCACCGGAATCTTGTCCCGCTTAGAGGTTGTTGTGTGGTCGAGGGGGAAGACAGTTACGAGGAGAAAGGAAGTCACAGATACCTTGTCTACGATTACATGCCGAATGGGAATCTAGATGACCATCTCTTTATTTCTCAGCCTAGTAGTCAGAGCGGAACTGAAAGGAGACCCTTGACTTGGCCTCAAAGAAAGAGCATAATCTTGGATGTAGCCAAGGGTTTAGCTTATCTTCACTATGGAGTGAAGCCTGCGATATATCACAGAGATATTAAGGCTACAAATATACTACTAGATGCCGATATGAGAGCGAGGGTGGCAGACTTTGGGCTTGTAAAACAGAGCTTGGAAGGCCAGTCTCATCTCACTACTAGAGTGGCAGGGACTCATGGATACTTAGCCCCTGAATATGCTCTTTATGGACAGCTGACTGAGAAGAGCGATGTTTATAGCTTTGGAGTGGTTATTTTGGAGATTATGTGTGGGAGAAAAGCACTTGATTTGTCATCTTCAGGTTCCCCTCGTGCTTTTTTGATCACAGATTGGGCATGGTCATTTGTGAAATCTGGAAAAGCTGAGCAAGCTTTAGACTTCTCATTGGTGAGAGATGGGGATAATGCGAATTCGAATCCAAAGAGCATAATGGAGAGATATTTGCTGGTCGGCATTTTGTGTGCTCACGTAATGGTGGCTTTACGGCCTACCATTTTGGAAGCCCTGAAAATGCTGGAGGGAGATATTGAGGTGCCACAGATTCCAGATAGGCCGATGCCTCTTGGTCATCCTTCAAGTTATGGCAATGGCAACGGTAACACGTTCAGCATATCACCAGCTTTGAGCGGGCCAAAATTACACAGCAGAGACATGCTCAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

672

Amino Acids

73.67

Weight (kDa)

7.48

Isoelectric Point (pI)

44.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPARK	PF19160	41 - 211	3.8e-41	SPARK
Pkinase	PF00069	330 - 547	1.1e-41	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	332 - 543	3.3e-40	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009218)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11050
fragaria_vesca	FvH4_5g29100 FvH4_5g29100 FvH4_5g29100
malus_domestica	MD08G1235700.v1.1 MD15G1427400.v1.1
prunus_persica	Prupe.1G571200_v2.0.a1 Prupe.1G571200_v2.0.a1
pyrus_communis	pycom08g20370 pycom15g37890
rosa_chinensis	RchiOBHm_Chr7g0221741
rosa_laevigata	RLG00000002134
rosa_multiflora	Rmu_co8451303.1_g000001 Rmu_sc0003311.1_g000001
rosa_roxburghii	Rroxscaffold_3G00238080
rosa_rugosa	Rorug07G0203100
rosa_samantha	Rh7AG344000 Rh7BG334500 Rh7CG361400 Rh7DG341800
rosa_wichuraiana	Rw0G003240 Rw7G029170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	191
Acc36I	ACCTGC	1 cut(s)	191
AccB1I	GGYRCC	1 cut(s)	1885
AccB7I	CCANNNNNTGG	1 cut(s)	477
AccBSI	CCGCTC	2 cut(s)	1296, 1980
AccI	GTMKAC	2 cut(s)	564, 1230
AciI	CCGC	8 cut(s)	516, 523, 552, 600, 794, 1160, 1296, 1980
AclWI	GGATC	3 cut(s)	150, 238, 287
AcsI	RAATTY	5 cut(s)	954, 983, 997, 1106, 1762
AcuI	CTGAAG	2 cut(s)	99, 1635
AcyI	GRCGYC	1 cut(s)	567
AfaI	GTAC	2 cut(s)	638, 827
AfiI	CCNNNNNNNGG	8 cut(s)	276, 477, 552, 553, 935, 970, 1166, 1320
AflIII	ACRYGT	1 cut(s)	1953
AgeI	ACCGGT	1 cut(s)	935
AgsI	TTSAA	5 cut(s)	172, 942, 1094, 1141, 1929
AjuI	GAANNNNNNNTTGG	2 cut(s)	922, 954
Alw21I	GWGCWC	1 cut(s)	1819
Alw26I	GTCTC	7 cut(s)	409, 487, 876, 919, 1303, 1503, 1995
AlwI	GGATC	3 cut(s)	150, 238, 287
AlwNI	CAGNNNCTG	1 cut(s)	77
AoxI	GGCC	7 cut(s)	679, 772, 1322, 1492, 1838, 1904, 1982
ApeKI	GCWGC	4 cut(s)	74, 128, 252, 656
ApoI	RAATTY	5 cut(s)	954, 983, 997, 1106, 1762
ArsI	GACNNNNNNTTYG	2 cut(s)	557, 589
AsiGI	ACCGGT	1 cut(s)	935
AspLEI	GCGC	3 cut(s)	455, 736, 785
AspS9I	GGNCC	4 cut(s)	232, 680, 961, 1982
AsuHPI	GGTGA	3 cut(s)	229, 1753, 1959
AsuII	TTCGAA	1 cut(s)	1766
AvaII	GGWCC	2 cut(s)	232, 961
BaeGI	GKGCMC	1 cut(s)	137
BanI	GGYRCC	1 cut(s)	1885
BauI	CACGAG	1 cut(s)	1662
BbsI	GAAGAC	2 cut(s)	714, 1197
Bbv12I	GWGCWC	1 cut(s)	1819
BbvI	GCAGC	4 cut(s)	86, 140, 239, 643
BccI	CCATC	5 cut(s)	73, 599, 745, 1272, 1742
BceAI	ACGGC	1 cut(s)	1853
BciVI	GTATCC	1 cut(s)	1526
BclI	TGATCA	1 cut(s)	1674
BcoDI	GTCTC	7 cut(s)	409, 487, 876, 919, 1303, 1503, 1995
BfaI	CTAG	5 cut(s)	912, 1256, 1284, 1433, 1512
BfoI	RGCGCY	1 cut(s)	786
BfuAI	ACCTGC	1 cut(s)	191
BfuI	GTATCC	1 cut(s)	1526
BglI	GCCNNNNNGGC	1 cut(s)	780
BisI	GCNGC	6 cut(s)	75, 129, 253, 523, 600, 657
BlpI	GCTNAGC	1 cut(s)	1716
BlsI	GCNGC	6 cut(s)	76, 130, 254, 524, 601, 658
Bme18I	GGWCC	2 cut(s)	232, 961
BmgT120I	GGNCC	4 cut(s)	232, 680, 961, 1982
BmiI	GGNNCC	2 cut(s)	1657, 1887
BmsI	GCATC	4 cut(s)	898, 1090, 1426, 1899
BpiI	GAAGAC	2 cut(s)	714, 1197
BplI	GAGNNNNNCTC	1 cut(s)	1992
BpmI	CTGGAG	2 cut(s)	410, 1889
Bpu10I	CCTNAGC	1 cut(s)	2009
Bpu1102I	GCTNAGC	1 cut(s)	1716
Bpu14I	TTCGAA	1 cut(s)	1766
BpuEI	CTTGAG	1 cut(s)	986
BsaAI	YACGTR	1 cut(s)	1822
BsaBI	GATNNNNATC	1 cut(s)	1263
BsaHI	GRCGYC	1 cut(s)	567
BsaI	GGTCTC	4 cut(s)	487, 876, 919, 1303
BsaJI	CCNNGG	1 cut(s)	1356
BsaWI	WCCGGW	3 cut(s)	884, 935, 1146
BsaXI	ACNNNNNCTCC	4 cut(s)	46, 76, 1604, 1634
Bsc4I	CCNNNNNNNGG	8 cut(s)	276, 477, 552, 553, 935, 970, 1166, 1320
Bse118I	RCCGGY	2 cut(s)	569, 935
Bse1I	ACTGG	5 cut(s)	364, 427, 471, 784, 1495
Bse3DI	GCAATG	3 cut(s)	697, 1120, 1945
Bse8I	GATNNNNATC	1 cut(s)	1263
BseDI	CCNNGG	1 cut(s)	1356
BseGI	GGATG	4 cut(s)	904, 1105, 1354, 1921
BseJI	GATNNNNATC	1 cut(s)	1263
BseLI	CCNNNNNNNGG	8 cut(s)	276, 477, 552, 553, 935, 970, 1166, 1320
BseMI	GCAATG	3 cut(s)	697, 1120, 1945
BseMII	CTCAG	5 cut(s)	137, 675, 1292, 1563, 1707
BseNI	ACTGG	5 cut(s)	364, 427, 471, 784, 1495
BseRI	GAGGAG	5 cut(s)	87, 90, 166, 367, 1217
BseSI	GKGCMC	1 cut(s)	137
BseXI	GCAGC	4 cut(s)	86, 140, 239, 643
BseYI	CCCAGC	1 cut(s)	785
Bsh1285I	CGRYCG	1 cut(s)	1009
BshFI	GGCC	7 cut(s)	681, 774, 1324, 1494, 1840, 1906, 1984
BshNI	GGYRCC	1 cut(s)	1885
BshTI	ACCGGT	1 cut(s)	935
BsiEI	CGRYCG	1 cut(s)	1009
BsiHKAI	GWGCWC	1 cut(s)	1819
BsiSI	CCGG	4 cut(s)	570, 885, 936, 1147
BslFI	GGGAC	4 cut(s)	253, 1066, 1142, 1537
BslI	CCNNNNNNNGG	8 cut(s)	276, 477, 552, 553, 935, 970, 1166, 1320
BsmAI	GTCTC	7 cut(s)	409, 487, 876, 919, 1303, 1503, 1995
BsmFI	GGGAC	4 cut(s)	253, 1066, 1142, 1537
BsnI	GGCC	7 cut(s)	681, 774, 1324, 1494, 1840, 1906, 1984
Bso31I	GGTCTC	4 cut(s)	487, 876, 919, 1303
Bsp119I	TTCGAA	1 cut(s)	1766
Bsp1286I	GDGCHC	2 cut(s)	137, 1819
Bsp143I	GATC	5 cut(s)	142, 243, 279, 538, 1674
Bsp1720I	GCTNAGC	1 cut(s)	1716
BspACI	CCGC	8 cut(s)	516, 523, 552, 600, 794, 1160, 1296, 1980
BspANI	GGCC	7 cut(s)	681, 774, 1324, 1494, 1840, 1906, 1984
BspCNI	CTCAG	5 cut(s)	138, 676, 1291, 1564, 1708
BspLI	GGNNCC	2 cut(s)	1657, 1887
BspMI	ACCTGC	1 cut(s)	191
BspPI	GGATC	3 cut(s)	150, 238, 287
BspQI	GCTCTTC	1 cut(s)	1571
BspT104I	TTCGAA	1 cut(s)	1766
BspT107I	GGYRCC	1 cut(s)	1885
BspTNI	GGTCTC	4 cut(s)	487, 876, 919, 1303
BsrBI	CCGCTC	2 cut(s)	1296, 1980
BsrDI	GCAATG	3 cut(s)	697, 1120, 1945
BsrFI	RCCGGY	2 cut(s)	569, 935
BsrI	ACTGG	5 cut(s)	364, 427, 471, 784, 1495
BssAI	RCCGGY	2 cut(s)	569, 935
BssECI	CCNNGG	1 cut(s)	1356
BssMI	GATC	5 cut(s)	142, 243, 279, 538, 1674
BssNI	GRCGYC	1 cut(s)	567
BssSI	CACGAG	1 cut(s)	1662
BssT1I	CCWWGG	1 cut(s)	1356
Bst2BI	CACGAG	1 cut(s)	1662
Bst4CI	ACNGT	6 cut(s)	505, 632, 707, 1060, 1196, 1949
Bst6I	CTCTTC	4 cut(s)	278, 320, 619, 1571
BstACI	GRCGYC	1 cut(s)	567
BstBAI	YACGTR	1 cut(s)	1822
BstBI	TTCGAA	1 cut(s)	1766
BstC8I	GCNNGC	6 cut(s)	133, 520, 571, 1392, 1722, 1982
BstDEI	CTNAG	9 cut(s)	146, 158, 684, 1163, 1278, 1537, 1572, 1716, 2009
BstENI	CCTNNNNNAGG	1 cut(s)	968
BstF5I	GGATG	4 cut(s)	904, 1105, 1354, 1921
BstH2I	RGCGCY	1 cut(s)	786
BstHHI	GCGC	3 cut(s)	455, 736, 785
BstKTI	GATC	5 cut(s)	145, 246, 282, 541, 1677
BstMAI	GTCTC	7 cut(s)	409, 487, 876, 919, 1303, 1503, 1995
BstMBI	GATC	5 cut(s)	142, 243, 279, 538, 1674
BstMCI	CGRYCG	1 cut(s)	1009
BstMWI	GCNNNNNNNGC	8 cut(s)	80, 575, 687, 780, 791, 1457, 1491, 1721
BstNSI	RCATGY	2 cut(s)	1243, 2008
BstSLI	GKGCMC	1 cut(s)	137
BstV1I	GCAGC	4 cut(s)	86, 140, 239, 643
BstV2I	GAAGAC	2 cut(s)	714, 1197
BstX2I	RGATCY	3 cut(s)	142, 243, 279
BstXI	CCANNNNNNTGG	1 cut(s)	220
BstYI	RGATCY	3 cut(s)	142, 243, 279
BsuI	GTATCC	1 cut(s)	1526
BsuRI	GGCC	7 cut(s)	681, 774, 1324, 1494, 1840, 1906, 1984
BtgZI	GCGATG	1 cut(s)	1596
BtsCI	GGATG	4 cut(s)	904, 1105, 1354, 1921
BtsIMutI	CAGTG	2 cut(s)	478, 777
BveI	ACCTGC	1 cut(s)	191
Cac8I	GCNNGC	6 cut(s)	133, 520, 571, 1392, 1722, 1982
CaiI	CAGNNNCTG	1 cut(s)	77
CfoI	GCGC	3 cut(s)	455, 736, 785
Cfr10I	RCCGGY	2 cut(s)	569, 935
Cfr13I	GGNCC	4 cut(s)	232, 680, 961, 1982
CseI	GACGC	1 cut(s)	575
Csp6I	GTAC	2 cut(s)	637, 826
CspAI	ACCGGT	1 cut(s)	935
CviAII	CATG	5 cut(s)	805, 1240, 1529, 1689, 2005
CviQI	GTAC	2 cut(s)	637, 826
DdeI	CTNAG	9 cut(s)	146, 158, 684, 1163, 1278, 1537, 1572, 1716, 2009
DpnI	GATC	5 cut(s)	144, 245, 281, 540, 1676
DpnII	GATC	5 cut(s)	142, 243, 279, 538, 1674
DraI	TTTAAA	1 cut(s)	952
Eam1104I	CTCTTC	4 cut(s)	278, 320, 619, 1571
EarI	CTCTTC	4 cut(s)	278, 320, 619, 1571
EciI	GGCGGA	1 cut(s)	505
Eco130I	CCWWGG	1 cut(s)	1356
Eco31I	GGTCTC	4 cut(s)	487, 876, 919, 1303
Eco47I	GGWCC	2 cut(s)	232, 961
Eco57I	CTGAAG	2 cut(s)	99, 1635
EcoNI	CCTNNNNNAGG	1 cut(s)	968
EcoO109I	RGGNCCY	2 cut(s)	232, 961
EcoRI	GAATTC	1 cut(s)	1762
EcoT14I	CCWWGG	1 cut(s)	1356
ErhI	CCWWGG	1 cut(s)	1356
FaeI	CATG	5 cut(s)	808, 1243, 1532, 1692, 2008
FaqI	GGGAC	4 cut(s)	253, 1066, 1142, 1537
FatI	CATG	5 cut(s)	804, 1239, 1528, 1688, 2004
FauI	CCCGC	3 cut(s)	545, 1167, 1973
FbaI	TGATCA	1 cut(s)	1674
FblI	GTMKAC	2 cut(s)	564, 1230
Fnu4HI	GCNGC	6 cut(s)	75, 129, 253, 523, 600, 657
FokI	GGATG	4 cut(s)	911, 1112, 1361, 1908
Fsp4HI	GCNGC	6 cut(s)	75, 129, 253, 523, 600, 657
FspBI	CTAG	5 cut(s)	912, 1256, 1284, 1433, 1512
GlaI	GCGC	3 cut(s)	454, 735, 784
GluI	GCNGC	6 cut(s)	75, 129, 253, 523, 600, 657
GsaI	CCCAGC	1 cut(s)	789
GsuI	CTGGAG	2 cut(s)	410, 1889
HaeII	RGCGCY	1 cut(s)	786
HaeIII	GGCC	7 cut(s)	681, 774, 1324, 1494, 1840, 1906, 1984
HapII	CCGG	4 cut(s)	570, 885, 936, 1147
HgaI	GACGC	1 cut(s)	575
HhaI	GCGC	3 cut(s)	455, 736, 785
Hin1I	GRCGYC	1 cut(s)	567
Hin1II	CATG	5 cut(s)	808, 1243, 1532, 1692, 2008
Hin6I	GCGC	3 cut(s)	453, 734, 783
HinP1I	GCGC	3 cut(s)	453, 734, 783
HincII	GTYRAC	1 cut(s)	565
HindII	GTYRAC	1 cut(s)	565
HindIII	AAGCTT	1 cut(s)	1722
HpaII	CCGG	4 cut(s)	570, 885, 936, 1147
HphI	GGTGA	3 cut(s)	229, 1753, 1959
Hpy166II	GTNNAC	5 cut(s)	468, 565, 703, 1030, 1231
Hpy188I	TCNGA	5 cut(s)	147, 229, 585, 1087, 1293
Hpy188III	TCNNGA	6 cut(s)	277, 873, 959, 1256, 1707, 1898
Hpy8I	GTNNAC	5 cut(s)	468, 565, 703, 1030, 1231
Hpy99I	CGWCG	2 cut(s)	569, 745
HpyAV	CCTTC	5 cut(s)	178, 213, 964, 1484, 1935
HpyCH4III	ACNGT	6 cut(s)	505, 632, 707, 1060, 1196, 1949
HpyCH4IV	ACGT	2 cut(s)	1821, 1955
HpyCH4V	TGCA	4 cut(s)	14, 186, 1045, 1113
HpyF10VI	GCNNNNNNNGC	8 cut(s)	80, 575, 687, 780, 791, 1457, 1491, 1721
HpyF3I	CTNAG	9 cut(s)	146, 158, 684, 1163, 1278, 1537, 1572, 1716, 2009
HpySE526I	ACGT	2 cut(s)	1821, 1955
Hsp92I	GRCGYC	1 cut(s)	567
Hsp92II	CATG	5 cut(s)	808, 1243, 1532, 1692, 2008
HspAI	GCGC	3 cut(s)	453, 734, 783
KroI	GCCGGC	1 cut(s)	569
KroNI	GCCGGC	1 cut(s)	571
Ksp22I	TGATCA	1 cut(s)	1674
Kzo9I	GATC	5 cut(s)	142, 243, 279, 538, 1674
LguI	GCTCTTC	1 cut(s)	1571
LmnI	GCTCC	1 cut(s)	100
Lsp1109I	GCAGC	4 cut(s)	86, 140, 239, 643
LweI	GCATC	4 cut(s)	898, 1090, 1426, 1899
MaeI	CTAG	5 cut(s)	912, 1256, 1284, 1433, 1512
MaeII	ACGT	2 cut(s)	1821, 1955
MaeIII	GTNAC	4 cut(s)	608, 1196, 1214, 1949
MalI	GATC	5 cut(s)	144, 245, 281, 540, 1676
MbiI	CCGCTC	2 cut(s)	1296, 1980
MboI	GATC	5 cut(s)	142, 243, 279, 538, 1674
MflI	RGATCY	3 cut(s)	142, 243, 279
MhlI	GDGCHC	2 cut(s)	137, 1819
MluCI	AATT	8 cut(s)	942, 954, 983, 997, 1106, 1135, 1762, 1988
MlyI	GAGTC	3 cut(s)	233, 506, 1519
MmeI	TCCRAC	3 cut(s)	207, 220, 343
MroNI	GCCGGC	1 cut(s)	569
MseI	TTAA	3 cut(s)	951, 1068, 1413
MslI	CAYNNNNRTG	1 cut(s)	218
MspA1I	CMGCKG	2 cut(s)	77, 1567
MspI	CCGG	4 cut(s)	570, 885, 936, 1147
MwoI	GCNNNNNNNGC	8 cut(s)	80, 575, 687, 780, 791, 1457, 1491, 1721
NaeI	GCCGGC	1 cut(s)	571
NdeII	GATC	5 cut(s)	142, 243, 279, 538, 1674
NgoMIV	GCCGGC	1 cut(s)	569
NlaIII	CATG	5 cut(s)	808, 1243, 1532, 1692, 2008
NlaIV	GGNNCC	2 cut(s)	1657, 1887
NmeAIII	GCCGAG	1 cut(s)	471
NmuCI	GTSAC	1 cut(s)	1214
NspI	RCATGY	2 cut(s)	1243, 2008
NspV	TTCGAA	1 cut(s)	1766
PaqCI	CACCTGC	1 cut(s)	191
PciSI	GCTCTTC	1 cut(s)	1571
PdiI	GCCGGC	1 cut(s)	571
PfeI	GAWTC	7 cut(s)	162, 881, 1082, 1150, 1252, 1768, 1894
PflMI	CCANNNNNTGG	1 cut(s)	477
PinAI	ACCGGT	1 cut(s)	935
PkrI	GCNGC	6 cut(s)	76, 130, 254, 524, 601, 658
PleI	GAGTC	3 cut(s)	232, 506, 1519
PpsI	GAGTC	3 cut(s)	232, 506, 1519
Ppu21I	YACGTR	1 cut(s)	1822
PpuMI	RGGWCCY	2 cut(s)	232, 961
Psp5II	RGGWCCY	2 cut(s)	232, 961
PspFI	CCCAGC	1 cut(s)	785
PspN4I	GGNNCC	2 cut(s)	1657, 1887
PspPI	GGNCC	4 cut(s)	232, 680, 961, 1982
PspPPI	RGGWCCY	2 cut(s)	232, 961
PstNI	CAGNNNCTG	1 cut(s)	77
PsuI	RGATCY	3 cut(s)	142, 243, 279
PvuII	CAGCTG	2 cut(s)	77, 1567
RsaI	GTAC	2 cut(s)	638, 827
RsaNI	GTAC	2 cut(s)	637, 826
RseI	CAYNNNNRTG	1 cut(s)	218
SalI	GTCGAC	1 cut(s)	563
SapI	GCTCTTC	1 cut(s)	1571
SaqAI	TTAA	3 cut(s)	951, 1068, 1413
SatI	GCNGC	6 cut(s)	75, 129, 253, 523, 600, 657
Sau3AI	GATC	5 cut(s)	142, 243, 279, 538, 1674
Sau96I	GGNCC	4 cut(s)	232, 680, 961, 1982
SchI	GAGTC	3 cut(s)	233, 506, 1519
SduI	GDGCHC	2 cut(s)	137, 1819
SfaNI	GCATC	4 cut(s)	898, 1090, 1426, 1899
SfuI	TTCGAA	1 cut(s)	1766
SinI	GGWCC	2 cut(s)	232, 961
SmiMI	CAYNNNNRTG	1 cut(s)	218
SmlI	CTYRAG	1 cut(s)	965
SmoI	CTYRAG	1 cut(s)	965
Sse9I	AATT	8 cut(s)	942, 954, 983, 997, 1106, 1135, 1762, 1988
SsiI	CCGC	8 cut(s)	516, 523, 552, 600, 794, 1160, 1296, 1980
SspMI	CTAG	5 cut(s)	912, 1256, 1284, 1433, 1512
StyI	CCWWGG	1 cut(s)	1356
TaaI	ACNGT	6 cut(s)	505, 632, 707, 1060, 1196, 1949
TaiI	ACGT	2 cut(s)	1824, 1958
TaqI	TCGA	8 cut(s)	68, 510, 564, 743, 874, 1009, 1182, 1766
TaqII	GACCGA	1 cut(s)	995
TasI	AATT	8 cut(s)	942, 954, 983, 997, 1106, 1135, 1762, 1988
TatI	WGTACW	1 cut(s)	825
TauI	GCSGC	2 cut(s)	525, 602
TfiI	GAWTC	7 cut(s)	162, 881, 1082, 1150, 1252, 1768, 1894
Tru1I	TTAA	3 cut(s)	951, 1068, 1413
Tru9I	TTAA	3 cut(s)	951, 1068, 1413
TscAI	CASTG	2 cut(s)	478, 784
TseFI	GTSAC	1 cut(s)	1214
TseI	GCWGC	4 cut(s)	74, 128, 252, 656
Tsp45I	GTSAC	1 cut(s)	1214
TspDTI	ATGAA	2 cut(s)	20, 915
TspRI	CASTG	2 cut(s)	478, 784
Van91I	CCANNNNNTGG	1 cut(s)	477
VpaK11BI	GGWCC	2 cut(s)	232, 961
XagI	CCTNNNNNAGG	1 cut(s)	968
XapI	RAATTY	5 cut(s)	954, 983, 997, 1106, 1762
XbaI	TCTAGA	1 cut(s)	1255
XceI	RCATGY	2 cut(s)	1243, 2008
XmiI	GTMKAC	2 cut(s)	564, 1230
XspI	CTAG	5 cut(s)	912, 1256, 1284, 1433, 1512

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.