Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0009218

Physical Location & Seq

Genomic Coordinates Forward (+)

20191454 .. 20193442

Gene Structure 1989 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom08g20370.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1989 bp

ATGATGAAACTTGCATTTGGGTTTTGGTTGTTTCTCTCTCTACTTTCTCTCTCAACCTTATCTCCATCAACCGCAGCAGAAACACCAGCTCCTCCCACAACCAACGTAACAACAACGGCAACATGTCCCTTGGATCTGAGCTATGTCCTAAGAATCCCCTTCAACTCCTCCACCTGCAAAAACTTCCAACCTCCTCCCAAAACCCCACAAATGGAGTCAGAGGACCCCGCCAATACCACCTGCTGCCAAACCCTTTTGTCCGTCTACGGAATCTCCCTCGCCCAACGCCTCAAAGAAACCTCCCTCTTCAACCTCCCCAACCTCCCCACCTCCATTTCCTGCCTCCAAAACTACCAGTCCAAGCTCTCCTCCCTCTCCTTCCCTCCCAATCTTGTCAACTACTGCTGGGACCCTCTCCAGTTTGTCATCACCCCCAACATCTGCGCCAACATTGAGTCCACCAGTGATTGGGTCTCCAAGCTCGGCAACTCCACCGTCCTCGACTCCGCCTGCCGCCCTGACCTCACCGATCAATCCTCCTGCGGGGCCTGCGTCGACGCCGGCTTTGCCACTCAGAAGATGCTGCTTGGCGTTGATGGTAACTCTTCTCACACTCGGGACTGTTGGTACTTTACTATTCTTTATGCTGCTGGTATTGTCAACGAGTTCGGCCCTGAGAGTAATGGTGCTGTGAACTGTATATTTGGGTTGTCGTTTGATTCGAATGCTTCGTCGAAAAAGAGCAATACCGCTCTTGTTTTCGGCCTCACTGGCGGTGGAGTTGCGGTGTTTGTTATGTGTAGTTTGTTGGGATTGTACTTTTGGTACTATAGGAGGTGGAAGAACAAAGGGATGGAATCCGGTTTCGACTTGGAAGAAGTAGATTCTAGGAGTAGAGTGAGACCAAACACTGGTTCAATTTGGTTTAAAATTCAGGACCTTGAGAAGGCAACTAATAACTTTTCGCAGAAGAATTTTATCGGTCGAGGTGGGTTTGGTGTTGTTTACAAGGGTGTGTTGCAGGATGGGACTACGGTTGCTGTTAAGAAAGTTATAGATTCTGATATGCAAGGGGATGCTGAGTTTTGCAATGAGGTTGAGATTATTAGCAATTTGAAGCACCGAAATCTTGTCCCGCTTAGAGGGTGTTGTGTGGTCGAGGGGGAAGACAATTATGAGGAGCAAGGAAGTCACAGGTACCTTGTCTACGATTACATGCCGAATGGGAATCTAGATGACCATCTCTTTACTTCTCAGCCAAGTAATCCGAGCGGAACTGAAAGGAGACCCTTGACTTGGCCTCAAAGAAAGAGCATAATATTGGATGTGGCCAAGGGTTTAGCTTATCTGCACTATGGAGTGAAGCCTGCAATATATCACAGAGATATTAAGGCGACAAACATACTACTAGATGCAGATATGAAAGCGAGGGTGGCGGACTTTGGGCTTGCAAAACAGAGCATGGAAGGCCAGTCCCATCTCACTACTAGAGTGGCAGGGACTCACGGGTACTTAGCCCCCGAATACGCTCTTTATGGACAGCTGACTGAGAAGAGCGATGTTTATAGCTTTGGAGTGGTTATTTTGGAGATCATGTGTGGGAGGAAAGCTCTTGATTTGTCTTTGGGATCCCCTCGTGCTTTTTTGATCACAGATTGGGCTTGGTCATTGGTGAAATCTGGAAAAGCCGAGCAGGCTTTAGACTTTTCATTGGTGAAAGATGGGGACAACGCGAATTCGAATCCAAAGAGCATAATGGAGAGATATTTGCTGGTCGGCATTTTGTGTGCTCATGTAATGGTGGCTTTACGGCCTACCATTTTGGATGCCCTGAAAATGTTGGAGGGAGATATTGAGGTGCCACGGATTCCAGATAGGCCGATGCCCTTTGGTCATCCTTCAAGTTATGGCAATGGCAACAGTAGCACGTTCAGCATATCACCAGCTTTGAGCGGCCCAAAATTACAAAGCGGAGACATGCTCAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

663

Amino Acids

72.66

Weight (kDa)

5.99

Isoelectric Point (pI)

43.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPARK	PF19160	38 - 208	2.6e-41	SPARK
Pkinase	PF00069	322 - 539	7.6e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	324 - 535	7.8e-42	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009218)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11050
fragaria_vesca	FvH4_5g29100 FvH4_5g29100 FvH4_5g29100
malus_domestica	MD08G1235700.v1.1 MD15G1427400.v1.1
prunus_persica	Prupe.1G571200_v2.0.a1 Prupe.1G571200_v2.0.a1
pyrus_communis	pycom08g20370 pycom15g37890
rosa_chinensis	RchiOBHm_Chr7g0221741
rosa_laevigata	RLG00000002134
rosa_multiflora	Rmu_co8451303.1_g000001 Rmu_sc0003311.1_g000001
rosa_roxburghii	Rroxscaffold_3G00238080
rosa_rugosa	Rorug07G0203100
rosa_samantha	Rh7AG344000 Rh7BG334500 Rh7CG361400 Rh7DG341800
rosa_wichuraiana	Rw0G003240 Rw7G029170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	182, 248
Acc36I	ACCTGC	2 cut(s)	182, 248
Acc65I	GGTACC	1 cut(s)	1197
AccB1I	GGYRCC	2 cut(s)	1197, 1858
AccB7I	CCANNNNNTGG	2 cut(s)	468, 911
AccBSI	CCGCTC	3 cut(s)	752, 1272, 1953
AccI	GTMKAC	3 cut(s)	264, 555, 1206
AccII	CGCG	1 cut(s)	1733
AclWI	GGATC	3 cut(s)	141, 1623, 1636
AcoI	YGGCCR	1 cut(s)	1329
AcsI	RAATTY	3 cut(s)	930, 973, 1735
AcyI	GRCGYC	1 cut(s)	558
AfaI	GTAC	5 cut(s)	629, 818, 827, 1199, 1511
AfiI	CCNNNNNNNGG	7 cut(s)	211, 468, 543, 911, 946, 1142, 1296
AflIII	ACRYGT	1 cut(s)	122
AgsI	TTSAA	5 cut(s)	163, 310, 918, 1117, 1902
AjuI	GAANNNNNNNTTGG	2 cut(s)	898, 930
AluBI	AGCT	9 cut(s)	89, 141, 364, 481, 1343, 1543, 1569, 1610, 1946
AluI	AGCT	9 cut(s)	89, 141, 364, 481, 1343, 1543, 1569, 1610, 1946
Alw21I	GWGCWC	1 cut(s)	1792
Alw26I	GTCTC	4 cut(s)	478, 895, 1279, 1968
AlwI	GGATC	3 cut(s)	141, 1623, 1636
Ama87I	CYCGRG	1 cut(s)	615
AoxI	GGCC	9 cut(s)	546, 670, 763, 1298, 1329, 1468, 1811, 1877, 1954
ApeKI	GCWGC	4 cut(s)	74, 243, 583, 647
ApoI	RAATTY	3 cut(s)	930, 973, 1735
ArsI	GACNNNNNNTTYG	4 cut(s)	548, 580, 716, 748
Asp718I	GGTACC	1 cut(s)	1197
AspLEI	GCGC	1 cut(s)	446
AspS9I	GGNCC	6 cut(s)	223, 409, 546, 671, 937, 1955
AsuHPI	GGTGA	5 cut(s)	421, 517, 1684, 1726, 1932
AsuII	TTCGAA	2 cut(s)	722, 1739
AvaI	CYCGRG	1 cut(s)	615
AvaII	GGWCC	3 cut(s)	223, 409, 937
BalI	TGGCCA	1 cut(s)	1331
BamHI	GGATCC	1 cut(s)	1628
BanI	GGYRCC	2 cut(s)	1197, 1858
BauI	CACGAG	1 cut(s)	1635
BbsI	GAAGAC	1 cut(s)	1173
Bbv12I	GWGCWC	1 cut(s)	1792
BbvI	GCAGC	4 cut(s)	86, 230, 570, 634
BccI	CCATC	7 cut(s)	73, 590, 847, 1019, 1248, 1485, 1715
BceAI	ACGGC	2 cut(s)	132, 1826
BclI	TGATCA	1 cut(s)	1647
BcoDI	GTCTC	4 cut(s)	478, 895, 1279, 1968
BfaI	CTAG	4 cut(s)	888, 1232, 1409, 1488
BfmI	CTRYAG	1 cut(s)	829
BfuAI	ACCTGC	2 cut(s)	182, 248
BglI	GCCNNNNNGGC	2 cut(s)	771, 1694
BisI	GCNGC	6 cut(s)	75, 244, 514, 584, 648, 1954
BlsI	GCNGC	6 cut(s)	76, 245, 515, 585, 649, 1955
Bme18I	GGWCC	3 cut(s)	223, 409, 937
BmeT110I	CYCGRG	1 cut(s)	615
BmgT120I	GGNCC	6 cut(s)	223, 409, 546, 671, 937, 1955
BmiI	GGNNCC	7 cut(s)	225, 410, 411, 547, 1199, 1630, 1860
BmsI	GCATC	5 cut(s)	570, 1066, 1402, 1816, 1872
BpiI	GAAGAC	1 cut(s)	1173
BplI	GAGNNNNNCTC	3 cut(s)	1594, 1626, 1965
BpmI	CTGGAG	1 cut(s)	401
Bpu10I	CCTNAGC	1 cut(s)	1982
Bpu14I	TTCGAA	2 cut(s)	722, 1739
BpuEI	CTTGAG	1 cut(s)	962
BsaBI	GATNNNNATC	1 cut(s)	1239
BsaHI	GRCGYC	1 cut(s)	558
BsaI	GGTCTC	3 cut(s)	478, 895, 1279
BsaJI	CCNNGG	3 cut(s)	129, 1332, 1862
BsaWI	WCCGGW	1 cut(s)	860
Bsc4I	CCNNNNNNNGG	7 cut(s)	211, 468, 543, 911, 946, 1142, 1296
Bse118I	RCCGGY	1 cut(s)	560
Bse1I	ACTGG	6 cut(s)	355, 418, 462, 775, 916, 1471
Bse3DI	GCAATG	2 cut(s)	1096, 1918
Bse8I	GATNNNNATC	1 cut(s)	1239
BseDI	CCNNGG	3 cut(s)	129, 1332, 1862
BseGI	GGATG	6 cut(s)	858, 1030, 1081, 1330, 1831, 1894
BseJI	GATNNNNATC	1 cut(s)	1239
BseLI	CCNNNNNNNGG	7 cut(s)	211, 468, 543, 911, 946, 1142, 1296
BseMI	GCAATG	2 cut(s)	1096, 1918
BseMII	CTCAG	6 cut(s)	128, 587, 666, 1071, 1268, 1539
BseNI	ACTGG	6 cut(s)	355, 418, 462, 775, 916, 1471
BseRI	GAGGAG	5 cut(s)	81, 157, 183, 358, 1193
BseXI	GCAGC	4 cut(s)	86, 230, 570, 634
BseYI	CCCAGC	1 cut(s)	405
BsgI	GTGCAG	1 cut(s)	1334
Bsh1236I	CGCG	1 cut(s)	1733
Bsh1285I	CGRYCG	1 cut(s)	985
BshFI	GGCC	9 cut(s)	548, 672, 765, 1300, 1331, 1470, 1813, 1879, 1956
BshNI	GGYRCC	2 cut(s)	1197, 1858
BsiEI	CGRYCG	1 cut(s)	985
BsiHKAI	GWGCWC	1 cut(s)	1792
BsiHKCI	CYCGRG	1 cut(s)	615
BsiSI	CCGG	2 cut(s)	561, 861
BslFI	GGGAC	8 cut(s)	111, 422, 632, 1042, 1118, 1459, 1513, 1739
BslI	CCNNNNNNNGG	7 cut(s)	211, 468, 543, 911, 946, 1142, 1296
BsmAI	GTCTC	4 cut(s)	478, 895, 1279, 1968
BsmFI	GGGAC	8 cut(s)	111, 422, 632, 1042, 1118, 1459, 1513, 1739
BsmI	GAATGC	1 cut(s)	730
BsnI	GGCC	9 cut(s)	548, 672, 765, 1300, 1331, 1470, 1813, 1879, 1956
Bso31I	GGTCTC	3 cut(s)	478, 895, 1279
BsoBI	CYCGRG	1 cut(s)	615
Bsp119I	TTCGAA	2 cut(s)	722, 1739
Bsp1286I	GDGCHC	1 cut(s)	1792
Bsp143I	GATC	5 cut(s)	133, 529, 1590, 1628, 1647
BspANI	GGCC	9 cut(s)	548, 672, 765, 1300, 1331, 1470, 1813, 1879, 1956
BspCNI	CTCAG	6 cut(s)	129, 586, 667, 1072, 1267, 1540
BspFNI	CGCG	1 cut(s)	1733
BspLI	GGNNCC	7 cut(s)	225, 410, 411, 547, 1199, 1630, 1860
BspMI	ACCTGC	2 cut(s)	182, 248
BspPI	GGATC	3 cut(s)	141, 1623, 1636
BspQI	GCTCTTC	1 cut(s)	1547
BspT104I	TTCGAA	2 cut(s)	722, 1739
BspT107I	GGYRCC	2 cut(s)	1197, 1858
BspTNI	GGTCTC	3 cut(s)	478, 895, 1279
BsrBI	CCGCTC	3 cut(s)	752, 1272, 1953
BsrDI	GCAATG	2 cut(s)	1096, 1918
BsrFI	RCCGGY	1 cut(s)	560
BsrI	ACTGG	6 cut(s)	355, 418, 462, 775, 916, 1471
BssAI	RCCGGY	1 cut(s)	560
BssECI	CCNNGG	3 cut(s)	129, 1332, 1862
BssMI	GATC	5 cut(s)	133, 529, 1590, 1628, 1647
BssNI	GRCGYC	1 cut(s)	558
BssSI	CACGAG	1 cut(s)	1635
BssT1I	CCWWGG	2 cut(s)	129, 1332
Bst2BI	CACGAG	1 cut(s)	1635
Bst4CI	ACNGT	5 cut(s)	496, 623, 698, 1036, 1922
Bst6I	CTCTTC	3 cut(s)	311, 610, 1547
BstACI	GRCGYC	1 cut(s)	558
BstBI	TTCGAA	2 cut(s)	722, 1739
BstC8I	GCNNGC	6 cut(s)	511, 550, 562, 1368, 1449, 1695
BstDSI	CCRYGG	1 cut(s)	1862
BstENI	CCTNNNNNAGG	1 cut(s)	944
BstF5I	GGATG	6 cut(s)	858, 1030, 1081, 1330, 1831, 1894
BstFNI	CGCG	1 cut(s)	1733
BstHHI	GCGC	1 cut(s)	446
BstKTI	GATC	5 cut(s)	136, 532, 1593, 1631, 1650
BstMAI	GTCTC	4 cut(s)	478, 895, 1279, 1968
BstMBI	GATC	5 cut(s)	133, 529, 1590, 1628, 1647
BstMCI	CGRYCG	1 cut(s)	985
BstMWI	GCNNNNNNNGC	8 cut(s)	549, 566, 771, 1433, 1467, 1694, 1923, 1932
BstNSI	RCATGY	3 cut(s)	126, 1219, 1981
BstSFI	CTRYAG	1 cut(s)	829
BstUI	CGCG	1 cut(s)	1733
BstV1I	GCAGC	4 cut(s)	86, 230, 570, 634
BstV2I	GAAGAC	1 cut(s)	1173
BstX2I	RGATCY	2 cut(s)	133, 1628
BstYI	RGATCY	2 cut(s)	133, 1628
BsuRI	GGCC	9 cut(s)	548, 672, 765, 1300, 1331, 1470, 1813, 1879, 1956
BtgI	CCRYGG	1 cut(s)	1862
BtgZI	GCGATG	1 cut(s)	1572
BtsCI	GGATG	6 cut(s)	858, 1030, 1081, 1330, 1831, 1894
BtsIMutI	CAGTG	3 cut(s)	469, 768, 909
BveI	ACCTGC	2 cut(s)	182, 248
Cac8I	GCNNGC	6 cut(s)	511, 550, 562, 1368, 1449, 1695
CfoI	GCGC	1 cut(s)	446
Cfr10I	RCCGGY	1 cut(s)	560
Cfr13I	GGNCC	6 cut(s)	223, 409, 546, 671, 937, 1955
CseI	GACGC	2 cut(s)	541, 566
Csp6I	GTAC	5 cut(s)	628, 817, 826, 1198, 1510
CviAII	CATG	6 cut(s)	123, 1216, 1462, 1594, 1793, 1978
CviQI	GTAC	5 cut(s)	628, 817, 826, 1198, 1510
DpnI	GATC	5 cut(s)	135, 531, 1592, 1630, 1649
DpnII	GATC	5 cut(s)	133, 529, 1590, 1628, 1647
DraI	TTTAAA	1 cut(s)	928
EaeI	YGGCCR	1 cut(s)	1329
Eam1104I	CTCTTC	3 cut(s)	311, 610, 1547
EarI	CTCTTC	3 cut(s)	311, 610, 1547
EciI	GGCGGA	2 cut(s)	496, 1451
Eco130I	CCWWGG	2 cut(s)	129, 1332
Eco31I	GGTCTC	3 cut(s)	478, 895, 1279
Eco47I	GGWCC	3 cut(s)	223, 409, 937
Eco88I	CYCGRG	1 cut(s)	615
EcoNI	CCTNNNNNAGG	1 cut(s)	944
EcoO109I	RGGNCCY	4 cut(s)	223, 409, 546, 937
EcoRI	GAATTC	1 cut(s)	1735
EcoT14I	CCWWGG	2 cut(s)	129, 1332
ErhI	CCWWGG	2 cut(s)	129, 1332
FaeI	CATG	6 cut(s)	126, 1219, 1465, 1597, 1796, 1981
FaqI	GGGAC	8 cut(s)	111, 422, 632, 1042, 1118, 1459, 1513, 1739
FatI	CATG	6 cut(s)	122, 1215, 1461, 1593, 1792, 1977
FauI	CCCGC	3 cut(s)	235, 536, 1143
FbaI	TGATCA	1 cut(s)	1647
FblI	GTMKAC	3 cut(s)	264, 555, 1206
Fnu4HI	GCNGC	6 cut(s)	75, 244, 514, 584, 648, 1954
FokI	GGATG	6 cut(s)	865, 1037, 1088, 1337, 1838, 1881
Fsp4HI	GCNGC	6 cut(s)	75, 244, 514, 584, 648, 1954
FspBI	CTAG	4 cut(s)	888, 1232, 1409, 1488
GlaI	GCGC	1 cut(s)	445
GluI	GCNGC	6 cut(s)	75, 244, 514, 584, 648, 1954
GsaI	CCCAGC	1 cut(s)	409
GsuI	CTGGAG	1 cut(s)	401
HaeIII	GGCC	9 cut(s)	548, 672, 765, 1300, 1331, 1470, 1813, 1879, 1956
HapII	CCGG	2 cut(s)	561, 861
HgaI	GACGC	2 cut(s)	541, 566
HhaI	GCGC	1 cut(s)	446
Hin1I	GRCGYC	1 cut(s)	558
Hin1II	CATG	6 cut(s)	126, 1219, 1465, 1597, 1796, 1981
Hin6I	GCGC	1 cut(s)	444
HinP1I	GCGC	1 cut(s)	444
HincII	GTYRAC	3 cut(s)	397, 556, 661
HindII	GTYRAC	3 cut(s)	397, 556, 661
HpaII	CCGG	2 cut(s)	561, 861
HphI	GGTGA	5 cut(s)	421, 517, 1684, 1726, 1932
Hpy166II	GTNNAC	8 cut(s)	265, 397, 459, 556, 661, 694, 1006, 1207
Hpy188I	TCNGA	5 cut(s)	138, 220, 576, 1063, 1269
Hpy188III	TCNNGA	6 cut(s)	617, 935, 1232, 1613, 1680, 1871
Hpy8I	GTNNAC	8 cut(s)	265, 397, 459, 556, 661, 694, 1006, 1207
Hpy99I	CGWCG	3 cut(s)	557, 560, 736
HpyAV	CCTTC	5 cut(s)	169, 388, 940, 1460, 1908
HpyCH4III	ACNGT	5 cut(s)	496, 623, 698, 1036, 1922
HpyCH4IV	ACGT	2 cut(s)	105, 1928
HpyCH4V	TGCA	9 cut(s)	14, 177, 1021, 1069, 1089, 1351, 1370, 1415, 1451
HpyF10VI	GCNNNNNNNGC	8 cut(s)	549, 566, 771, 1433, 1467, 1694, 1923, 1932
HpySE526I	ACGT	2 cut(s)	105, 1928
Hsp92I	GRCGYC	1 cut(s)	558
Hsp92II	CATG	6 cut(s)	126, 1219, 1465, 1597, 1796, 1981
HspAI	GCGC	1 cut(s)	444
KflI	GGGWCCC	1 cut(s)	409
KpnI	GGTACC	1 cut(s)	1201
KroI	GCCGGC	1 cut(s)	560
KroNI	GCCGGC	1 cut(s)	562
Ksp22I	TGATCA	1 cut(s)	1647
Kzo9I	GATC	5 cut(s)	133, 529, 1590, 1628, 1647
LguI	GCTCTTC	1 cut(s)	1547
LmnI	GCTCC	2 cut(s)	94, 1180
Lsp1109I	GCAGC	4 cut(s)	86, 230, 570, 634
LweI	GCATC	5 cut(s)	570, 1066, 1402, 1816, 1872
MaeI	CTAG	4 cut(s)	888, 1232, 1409, 1488
MaeII	ACGT	2 cut(s)	105, 1928
MaeIII	GTNAC	3 cut(s)	106, 599, 1190
MalI	GATC	5 cut(s)	135, 531, 1592, 1630, 1649
MbiI	CCGCTC	3 cut(s)	752, 1272, 1953
MboI	GATC	5 cut(s)	133, 529, 1590, 1628, 1647
MboII	GAAGA	8 cut(s)	298, 589, 597, 853, 887, 982, 1178, 1564
MflI	RGATCY	2 cut(s)	133, 1628
MhlI	GDGCHC	1 cut(s)	1792
MlsI	TGGCCA	1 cut(s)	1331
MluCI	AATT	7 cut(s)	918, 930, 973, 1111, 1171, 1735, 1961
MluNI	TGGCCA	1 cut(s)	1331
MlyI	GAGTC	4 cut(s)	224, 464, 497, 1495
MmeI	TCCRAC	2 cut(s)	211, 1821
Mox20I	TGGCCA	1 cut(s)	1331
MroNI	GCCGGC	1 cut(s)	560
MscI	TGGCCA	1 cut(s)	1331
MseI	TTAA	3 cut(s)	927, 1044, 1389
Msp20I	TGGCCA	1 cut(s)	1331
MspA1I	CMGCKG	1 cut(s)	1543
MspI	CCGG	2 cut(s)	561, 861
Mva1269I	GAATGC	1 cut(s)	730
MvnI	CGCG	1 cut(s)	1733
MwoI	GCNNNNNNNGC	8 cut(s)	549, 566, 771, 1433, 1467, 1694, 1923, 1932
NaeI	GCCGGC	1 cut(s)	562
NdeII	GATC	5 cut(s)	133, 529, 1590, 1628, 1647
NgoMIV	GCCGGC	1 cut(s)	560
NlaIII	CATG	6 cut(s)	126, 1219, 1465, 1597, 1796, 1981
NlaIV	GGNNCC	7 cut(s)	225, 410, 411, 547, 1199, 1630, 1860
NmeAIII	GCCGAG	2 cut(s)	462, 1714
NmuCI	GTSAC	1 cut(s)	1190
NspI	RCATGY	3 cut(s)	126, 1219, 1981
NspV	TTCGAA	2 cut(s)	722, 1739
PaqCI	CACCTGC	2 cut(s)	182, 248
PciI	ACATGT	1 cut(s)	122
PciSI	GCTCTTC	1 cut(s)	1547
PcsI	WCGNNNNNNNCGW	2 cut(s)	719, 728
PctI	GAATGC	1 cut(s)	730
PdiI	GCCGGC	1 cut(s)	562
PfeI	GAWTC	9 cut(s)	153, 270, 719, 857, 884, 1058, 1228, 1741, 1867
PflMI	CCANNNNNTGG	2 cut(s)	468, 911
PkrI	GCNGC	6 cut(s)	76, 245, 515, 585, 649, 1955
PleI	GAGTC	4 cut(s)	223, 463, 497, 1495
PpsI	GAGTC	4 cut(s)	223, 463, 497, 1495
PpuMI	RGGWCCY	3 cut(s)	223, 409, 937
PscI	ACATGT	1 cut(s)	122
Psp5II	RGGWCCY	3 cut(s)	223, 409, 937
PspFI	CCCAGC	1 cut(s)	405
PspN4I	GGNNCC	7 cut(s)	225, 410, 411, 547, 1199, 1630, 1860
PspPI	GGNCC	6 cut(s)	223, 409, 546, 671, 937, 1955
PspPPI	RGGWCCY	3 cut(s)	223, 409, 937
PsuI	RGATCY	2 cut(s)	133, 1628
PvuII	CAGCTG	1 cut(s)	1543
RsaI	GTAC	5 cut(s)	629, 818, 827, 1199, 1511
RsaNI	GTAC	5 cut(s)	628, 817, 826, 1198, 1510
SalI	GTCGAC	1 cut(s)	554
SapI	GCTCTTC	1 cut(s)	1547
SaqAI	TTAA	3 cut(s)	927, 1044, 1389
SatI	GCNGC	6 cut(s)	75, 244, 514, 584, 648, 1954
Sau3AI	GATC	5 cut(s)	133, 529, 1590, 1628, 1647
Sau96I	GGNCC	6 cut(s)	223, 409, 546, 671, 937, 1955
SchI	GAGTC	4 cut(s)	224, 464, 497, 1495
SduI	GDGCHC	1 cut(s)	1792
SfaNI	GCATC	5 cut(s)	570, 1066, 1402, 1816, 1872
SfcI	CTRYAG	1 cut(s)	829
SfuI	TTCGAA	2 cut(s)	722, 1739
SgrDI	CGTCGACG	1 cut(s)	554
SinI	GGWCC	3 cut(s)	223, 409, 937
SmlI	CTYRAG	1 cut(s)	941
SmoI	CTYRAG	1 cut(s)	941
Sse9I	AATT	7 cut(s)	918, 930, 973, 1111, 1171, 1735, 1961
SspI	AATATT	1 cut(s)	1320
SspMI	CTAG	4 cut(s)	888, 1232, 1409, 1488
StyI	CCWWGG	2 cut(s)	129, 1332
TaaI	ACNGT	5 cut(s)	496, 623, 698, 1036, 1922
TaiI	ACGT	2 cut(s)	108, 1931
TaqI	TCGA	8 cut(s)	501, 555, 722, 734, 867, 985, 1158, 1739
TaqII	GACCGA	1 cut(s)	971
TasI	AATT	7 cut(s)	918, 930, 973, 1111, 1171, 1735, 1961
TatI	WGTACW	1 cut(s)	816
TauI	GCSGC	2 cut(s)	516, 1956
TfiI	GAWTC	9 cut(s)	153, 270, 719, 857, 884, 1058, 1228, 1741, 1867
Tru1I	TTAA	3 cut(s)	927, 1044, 1389
Tru9I	TTAA	3 cut(s)	927, 1044, 1389
TscAI	CASTG	3 cut(s)	469, 775, 916
TseFI	GTSAC	1 cut(s)	1190
TseI	GCWGC	4 cut(s)	74, 243, 583, 647
Tsp45I	GTSAC	1 cut(s)	1190
TspDTI	ATGAA	3 cut(s)	20, 1436, 1698
TspGWI	ACGGA	3 cut(s)	250, 282, 1879
TspRI	CASTG	3 cut(s)	469, 775, 916
Van91I	CCANNNNNTGG	2 cut(s)	468, 911
VpaK11BI	GGWCC	3 cut(s)	223, 409, 937
XagI	CCTNNNNNAGG	1 cut(s)	944
XapI	RAATTY	3 cut(s)	930, 973, 1735
XbaI	TCTAGA	1 cut(s)	1231
XceI	RCATGY	3 cut(s)	126, 1219, 1981
XmiI	GTMKAC	3 cut(s)	264, 555, 1206
XspI	CTAG	4 cut(s)	888, 1232, 1409, 1488

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.