Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0007233

Physical Location & Seq

Genomic Coordinates Reverse (-)

30657346 .. 30660320

Gene Structure 2975 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1240400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 672 bp

ATGAAAGCGGTGCTTCTCAATTCGCCGCCGTACTGCAGACTCCAACAACGACTTTCCACGAGTTGTAGTTTATTGTTTGGAGGTTCAGGCTTCTGTGTATCTTCCAGTAGGGGGCGTAATCTGGTGCGGCCTAGCTCACGGGTTCGGTTACCTTCACCTTCACGCAGCCTAGTTGTTAAGGCGGTTGCTACTCCGAATTCAGCGGTCGAATTGCCACTGACTGCAGAGAATGTTGAAAGTGTATTGGATGAAATCCGGCCATATCTCATTTCTGACGGAGGAAATGTGTCGTTACATGAGATTGATGGAAATGTAGTGCGGTTGAAGCTAGAGGGAGCATGTGGCTCCTGTCCAAGCTCTGTTGTGACAATGAAAATGGGTATTGAGCGTCGTTTAATGGAAAAGATCCCTGAAATAGTTGCAGTGGAAGCAATAGCTGACGAAGAAACAGGGCTTGAGCTGAATGAAGAAAATATAGAGAAGGTACTTGAAGAAATTAGACCCTACTTAGTAGGGGCAGCAGGTGGATCTCTTGAACTTGTGACAATCGAGGATCCTATAGTGAAGGTGCGTATCACAGGTCCAGCTGCTGGGGTAATGACTGTCAGGGTGGCGGTCACGCAGAAGTTGAGGGAGAAAATCCCGTCCATTGCAGCAGTTCAACTACTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006790 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009657 GO:0009658 GO:0009987 GO:0016020 GO:0016043 GO:0016226 GO:0022607 GO:0031163 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051186 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NifU

Protein Analysis

224

Amino Acids

23.91

Weight (kDa)

6.01

Isoelectric Point (pI)

56.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NifU	PF01106	78 - 143	1e-26	NifU-like domain
NifU	PF01106	159 - 220	5.2e-12	NifU-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007233)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49940 AT5G49940
fragaria_vesca	FvH4_7g01380 FvH4_7g01380 FvH4_7g01380 FvH4_7g01380 FvH4_7g01380
malus_domestica	MD08G1240400.v1.1 MD15G1433600.v1.1
prunus_persica	Prupe.1G577000_v2.0.a1
pyrus_communis	pycom08g20850 pycom15g38280
rosa_chinensis	RchiOBHm_Chr1g0317081
rosa_laevigata	RLG00000030628
rosa_multiflora	Rmu_co8218640.1_g000001 Rmu_sc0003689.1_g000019
rosa_roxburghii	Rroxscaffold_4G00330680
rosa_rugosa	Rorug01G0014300 Rorug01G0014400 Rorug01G0014500 Rorug01G0014600
rosa_samantha	Rh1AG025400 Rh1CG023800 Rh1DG022600
rosa_wichuraiana	Rw1G001770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	512
Acc36I	ACCTGC	1 cut(s)	512
AccB7I	CCANNNNNTGG	1 cut(s)	590
AciI	CCGC	7 cut(s)	8, 26, 127, 182, 203, 319, 614
AclWI	GGATC	4 cut(s)	400, 535, 548, 561
AcoI	YGGCCR	1 cut(s)	257
AcsI	RAATTY	1 cut(s)	196
AfaI	GTAC	2 cut(s)	32, 486
AfiI	CCNNNNNNNGG	2 cut(s)	111, 590
AgsI	TTSAA	5 cut(s)	236, 325, 491, 536, 662
AluBI	AGCT	6 cut(s)	135, 328, 357, 437, 460, 587
AluI	AGCT	6 cut(s)	135, 328, 357, 437, 460, 587
AlwI	GGATC	4 cut(s)	400, 535, 548, 561
AlwNI	CAGNNNCTG	1 cut(s)	590
AoxI	GGCC	2 cut(s)	128, 257
ApeKI	GCWGC	4 cut(s)	165, 518, 587, 653
ApoI	RAATTY	1 cut(s)	196
AspS9I	GGNCC	1 cut(s)	581
AsuHPI	GGTGA	1 cut(s)	147
AvaII	GGWCC	1 cut(s)	581
BamHI	GGATCC	1 cut(s)	553
BauI	CACGAG	1 cut(s)	58
BbvI	GCAGC	4 cut(s)	177, 530, 574, 665
BccI	CCATC	1 cut(s)	299
BceAI	ACGGC	1 cut(s)	13
BfaI	CTAG	3 cut(s)	132, 170, 329
BfmI	CTRYAG	3 cut(s)	34, 222, 558
BfuAI	ACCTGC	1 cut(s)	512
BisI	GCNGC	6 cut(s)	26, 128, 166, 519, 588, 654
BlsI	GCNGC	6 cut(s)	27, 129, 167, 520, 589, 655
Bme18I	GGWCC	1 cut(s)	581
BmgT120I	GGNCC	1 cut(s)	581
BmiI	GGNNCC	2 cut(s)	346, 555
BpuEI	CTTGAG	1 cut(s)	476
BsaXI	ACNNNNNCTCC	2 cut(s)	270, 300
Bsc4I	CCNNNNNNNGG	2 cut(s)	111, 590
Bse1I	ACTGG	1 cut(s)	105
Bse3DI	GCAATG	1 cut(s)	648
BseGI	GGATG	1 cut(s)	253
BseLI	CCNNNNNNNGG	2 cut(s)	111, 590
BseMI	GCAATG	1 cut(s)	648
BseNI	ACTGG	1 cut(s)	105
BseXI	GCAGC	4 cut(s)	177, 530, 574, 665
BseYI	CCCAGC	1 cut(s)	590
Bsh1285I	CGRYCG	1 cut(s)	207
BshFI	GGCC	2 cut(s)	130, 259
BsiEI	CGRYCG	1 cut(s)	207
BsiSI	CCGG	1 cut(s)	256
BslI	CCNNNNNNNGG	2 cut(s)	111, 590
BsnI	GGCC	2 cut(s)	130, 259
Bsp143I	GATC	3 cut(s)	405, 527, 553
BspACI	CCGC	7 cut(s)	8, 26, 127, 182, 203, 319, 614
BspANI	GGCC	2 cut(s)	130, 259
BspLI	GGNNCC	2 cut(s)	346, 555
BspMAI	CTGCAG	2 cut(s)	38, 226
BspMI	ACCTGC	1 cut(s)	512
BspPI	GGATC	4 cut(s)	400, 535, 548, 561
BsrDI	GCAATG	1 cut(s)	648
BsrI	ACTGG	1 cut(s)	105
BssMI	GATC	3 cut(s)	405, 527, 553
BssSI	CACGAG	1 cut(s)	58
Bst2BI	CACGAG	1 cut(s)	58
Bst4CI	ACNGT	2 cut(s)	604, 669
BstDEI	CTNAG	1 cut(s)	508
BstEII	GGTNACC	1 cut(s)	147
BstF5I	GGATG	1 cut(s)	253
BstKTI	GATC	3 cut(s)	408, 530, 556
BstMBI	GATC	3 cut(s)	405, 527, 553
BstMCI	CGRYCG	1 cut(s)	207
BstMWI	GCNNNNNNNGC	2 cut(s)	325, 428
BstNSI	RCATGY	1 cut(s)	342
BstPI	GGTNACC	1 cut(s)	147
BstSFI	CTRYAG	3 cut(s)	34, 222, 558
BstV1I	GCAGC	4 cut(s)	177, 530, 574, 665
BstX2I	RGATCY	3 cut(s)	405, 527, 553
BstYI	RGATCY	3 cut(s)	405, 527, 553
BsuRI	GGCC	2 cut(s)	130, 259
BtsCI	GGATG	1 cut(s)	253
BtsI	GCAGTG	1 cut(s)	429
BtsIMutI	CAGTG	2 cut(s)	215, 429
BveI	ACCTGC	1 cut(s)	512
CaiI	CAGNNNCTG	1 cut(s)	590
Cfr13I	GGNCC	1 cut(s)	581
CseI	GACGC	1 cut(s)	377
Csp6I	GTAC	2 cut(s)	31, 485
CviAII	CATG	2 cut(s)	296, 339
CviQI	GTAC	2 cut(s)	31, 485
DdeI	CTNAG	1 cut(s)	508
DpnI	GATC	3 cut(s)	407, 529, 555
DpnII	GATC	3 cut(s)	405, 527, 553
EaeI	YGGCCR	1 cut(s)	257
Eco47I	GGWCC	1 cut(s)	581
Eco91I	GGTNACC	1 cut(s)	147
EcoO65I	GGTNACC	1 cut(s)	147
EcoRI	GAATTC	1 cut(s)	196
FaeI	CATG	2 cut(s)	299, 342
FaiI	YATR	5 cut(s)	262, 297, 340, 476, 560
FalI	AAGNNNNNCTT	1 cut(s)	29
FatI	CATG	2 cut(s)	295, 338
Fnu4HI	GCNGC	6 cut(s)	26, 128, 166, 519, 588, 654
FokI	GGATG	1 cut(s)	260
Fsp4HI	GCNGC	6 cut(s)	26, 128, 166, 519, 588, 654
FspBI	CTAG	3 cut(s)	132, 170, 329
GluI	GCNGC	6 cut(s)	26, 128, 166, 519, 588, 654
GsaI	CCCAGC	1 cut(s)	594
HaeIII	GGCC	2 cut(s)	130, 259
HapII	CCGG	1 cut(s)	256
HgaI	GACGC	1 cut(s)	377
Hin1II	CATG	2 cut(s)	299, 342
HinfI	GANTC	1 cut(s)	39
HpaII	CCGG	1 cut(s)	256
HphI	GGTGA	1 cut(s)	147
Hpy188I	TCNGA	2 cut(s)	195, 274
Hpy188III	TCNNGA	1 cut(s)	533
Hpy99I	CGWCG	1 cut(s)	393
HpyAV	CCTTC	4 cut(s)	162, 168, 475, 559
HpyCH4III	ACNGT	2 cut(s)	604, 669
HpyCH4V	TGCA	4 cut(s)	36, 224, 422, 653
HpyF10VI	GCNNNNNNNGC	2 cut(s)	325, 428
HpyF3I	CTNAG	1 cut(s)	508
Hsp92II	CATG	2 cut(s)	299, 342
Kzo9I	GATC	3 cut(s)	405, 527, 553
LmnI	GCTCC	2 cut(s)	335, 350
Lsp1109I	GCAGC	4 cut(s)	177, 530, 574, 665
MaeI	CTAG	3 cut(s)	132, 170, 329
MaeIII	GTNAC	5 cut(s)	147, 291, 364, 541, 616
MalI	GATC	3 cut(s)	407, 529, 555
MboI	GATC	3 cut(s)	405, 527, 553
MboII	GAAGA	4 cut(s)	93, 455, 479, 503
MflI	RGATCY	3 cut(s)	405, 527, 553
MluCI	AATT	4 cut(s)	19, 196, 209, 495
MlyI	GAGTC	1 cut(s)	33
MmeI	TCCRAC	1 cut(s)	67
MnlI	CCTC	5 cut(s)	74, 272, 325, 544, 624
MseI	TTAA	2 cut(s)	177, 395
MspA1I	CMGCKG	2 cut(s)	203, 587
MspI	CCGG	1 cut(s)	256
MwoI	GCNNNNNNNGC	2 cut(s)	325, 428
NdeII	GATC	3 cut(s)	405, 527, 553
NlaIII	CATG	2 cut(s)	299, 342
NlaIV	GGNNCC	2 cut(s)	346, 555
NmuCI	GTSAC	3 cut(s)	364, 541, 616
NspI	RCATGY	1 cut(s)	342
PaqCI	CACCTGC	1 cut(s)	512
PflMI	CCANNNNNTGG	1 cut(s)	590
PkrI	GCNGC	6 cut(s)	27, 129, 167, 520, 589, 655
PleI	GAGTC	1 cut(s)	33
PpsI	GAGTC	1 cut(s)	33
PspEI	GGTNACC	1 cut(s)	147
PspFI	CCCAGC	1 cut(s)	590
PspN4I	GGNNCC	2 cut(s)	346, 555
PspPI	GGNCC	1 cut(s)	581
PstI	CTGCAG	2 cut(s)	38, 226
PstNI	CAGNNNCTG	1 cut(s)	590
PsuI	RGATCY	3 cut(s)	405, 527, 553
PvuII	CAGCTG	1 cut(s)	587
RsaI	GTAC	2 cut(s)	32, 486
RsaNI	GTAC	2 cut(s)	31, 485
SaqAI	TTAA	2 cut(s)	177, 395
SatI	GCNGC	6 cut(s)	26, 128, 166, 519, 588, 654
Sau3AI	GATC	3 cut(s)	405, 527, 553
Sau96I	GGNCC	1 cut(s)	581
SchI	GAGTC	1 cut(s)	33
SfcI	CTRYAG	3 cut(s)	34, 222, 558
SinI	GGWCC	1 cut(s)	581
SmlI	CTYRAG	1 cut(s)	455
SmoI	CTYRAG	1 cut(s)	455
Sse9I	AATT	4 cut(s)	19, 196, 209, 495
SsiI	CCGC	7 cut(s)	8, 26, 127, 182, 203, 319, 614
SspMI	CTAG	3 cut(s)	132, 170, 329
TaaI	ACNGT	2 cut(s)	604, 669
TaqI	TCGA	2 cut(s)	207, 549
TasI	AATT	4 cut(s)	19, 196, 209, 495
TauI	GCSGC	2 cut(s)	28, 130
Tru1I	TTAA	2 cut(s)	177, 395
Tru9I	TTAA	2 cut(s)	177, 395
TscAI	CASTG	2 cut(s)	222, 429
TseFI	GTSAC	3 cut(s)	364, 541, 616
TseI	GCWGC	4 cut(s)	165, 518, 587, 653
Tsp45I	GTSAC	3 cut(s)	364, 541, 616
TspDTI	ATGAA	4 cut(s)	17, 264, 386, 480
TspGWI	ACGGA	1 cut(s)	291
TspRI	CASTG	2 cut(s)	222, 429
Van91I	CCANNNNNTGG	1 cut(s)	590
VpaK11BI	GGWCC	1 cut(s)	581
XapI	RAATTY	1 cut(s)	196
XceI	RCATGY	1 cut(s)	342
XspI	CTAG	3 cut(s)	132, 170, 329

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.