MD08G1240500.v1.1

WEB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015330
Physical Location & Seq
Reverse (-)
30662287 .. 30663117
831 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1240500.v1.1.491

Sequence Viewer

Length: 831 bp
ATGGAATCGGAAACCCAGCACTCCCCGCAGCTTCCTCAAGAAAACCCAGCGGGAGGAGCAGCTGCCCCAACCCCACTCCATGTTAACGATCACCACGCCATTGTCGACACTTCCCGACAATTCAACTCCGTCAAAGAAGCCGTCGCCATCCTCGGCGAGCGGCTTCTTCTCAGAGAAATGTTTTCATCCCCTAAACCGCCATGCACCAATAATATTGTCCAAAGCAGACCTACTACTCCTCCGTCACGGAACTTGTCGCCGCCGAATTATGAAGAAAGCGAAGAAGTAAAAAATAGTAGTGATGGTGATCGCGAGGAGAAAGTAGCAGTTATGGACACTCTAAAGAAGCTTGAGGCGGAGCTGGAGGAAACGAAGGTGGAGCTGAAGATGCTGAAGGAGAGAGAGTCGGAAACCGAGGTCGCGTTGGCGTCGCTGAACGCGGAGCTTCACAAGAACATGTCCAAGTTGGCTCGAGCAGAGGCAGCTGCGGCGGCAAAGGCGAGCAACGTGCTGCAAGTGGCTACAACTACAAGATCATCACCAAATCTGAGAGAGAATGTTGAAGAGAGGAACAAGAGGGAGTGGATGATAAGGATGGAGGACTCTACGTCTTTGGCTCAAATATTGAACATTGGAACTACTAAGAAACAAGGAGGAGCCTGCTACGACCCTGTCGGAGCCGGCTACGACCACCTCGGAGAGAAAAGAGAGAAGAAACCAATGAAGAAGAAGCCTATTGTCCCTCTGGTGCAGGATTTACTTTGCTGGAGAAAAGGGTCGTCCACTGCTTTTCGCAACCCTCTTTATTCGTCTCCGCAATTGTACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

277

Amino Acids

30.7

Weight (kDa)

6.63

Isoelectric Point (pI)

62.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015330)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G58070
fragaria_vesca FvH4_6g51930
malus_domestica MD08G1240500.v1.1 MD15G1433700.v1.1
prunus_persica Prupe.1G577200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0173411
rosa_laevigata RLG00000022187
rosa_multiflora Rmu_co8519037.1_g000002 Rmu_ssc0000282.1_g000013
rosa_roxburghii Rroxscaffold_2G00078890
rosa_rugosa Rorug02G0568100
rosa_samantha Rh2AG648600 Rh2BG659200 Rh2CG624500 Rh2DG674400
rosa_wichuraiana Rw2G053240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 160
AccI GTMKAC 1 cut(s) 105
AccII CGCG 3 cut(s) 312, 422, 440
AcuI CTGAAG 2 cut(s) 404, 413
AcyI GRCGYC 1 cut(s) 428
AfaI GTAC 1 cut(s) 824
AfiI CCNNNNNNNGG 1 cut(s) 53
AflIII ACRYGT 1 cut(s) 456
AgsI TTSAA 3 cut(s) 124, 563, 628
AhdI GACNNNNNGTC 1 cut(s) 607
AluBI AGCT 7 cut(s) 31, 62, 349, 361, 382, 445, 485
AluI AGCT 7 cut(s) 31, 62, 349, 361, 382, 445, 485
Alw26I GTCTC 1 cut(s) 816
Ama87I CYCGRG 1 cut(s) 471
ApeKI GCWGC 6 cut(s) 28, 59, 62, 482, 485, 511
ArsI GACNNNNNNTTYG 2 cut(s) 126, 158
AsuHPI GGTGA 3 cut(s) 83, 317, 531
AvaI CYCGRG 1 cut(s) 471
BbvI GCAGC 6 cut(s) 40, 49, 71, 472, 494, 498
BccI CCATC 3 cut(s) 155, 296, 589
BceAI ACGGC 1 cut(s) 125
BcgI CGANNNNNNTGC 2 cut(s) 490, 524
BcoDI GTCTC 1 cut(s) 816
BmeRI GACNNNNNGTC 1 cut(s) 607
BmeT110I CYCGRG 1 cut(s) 471
BmiI GGNNCC 2 cut(s) 658, 679
BmsI GCATC 1 cut(s) 378
BpmI CTGGAG 2 cut(s) 383, 787
BpuEI CTTGAG 2 cut(s) 21, 371
BsaBI GATNNNNATC 1 cut(s) 306
BsaHI GRCGYC 1 cut(s) 428
BsaJI CCNNGG 3 cut(s) 151, 414, 694
BsaXI ACNNNNNCTCC 4 cut(s) 223, 253, 389, 419
Bsc4I CCNNNNNNNGG 1 cut(s) 53
Bse118I RCCGGY 1 cut(s) 680
Bse8I GATNNNNATC 1 cut(s) 306
BseDI CCNNGG 3 cut(s) 151, 414, 694
BseGI GGATG 4 cut(s) 147, 185, 591, 600
BseJI GATNNNNATC 1 cut(s) 306
BseLI CCNNNNNNNGG 1 cut(s) 53
BseMII CTCAG 2 cut(s) 184, 539
BseRI GAGGAG 4 cut(s) 69, 228, 329, 669
BseXI GCAGC 6 cut(s) 40, 49, 71, 472, 494, 498
BseYI CCCAGC 2 cut(s) 15, 46
BsgI GTGCAG 1 cut(s) 770
Bsh1236I CGCG 3 cut(s) 312, 422, 440
BsiHKCI CYCGRG 1 cut(s) 471
BsiSI CCGG 1 cut(s) 681
BslFI GGGAC 1 cut(s) 725
BslI CCNNNNNNNGG 1 cut(s) 53
BsmAI GTCTC 1 cut(s) 816
BsmBI CGTCTC 1 cut(s) 816
BsmFI GGGAC 1 cut(s) 725
BsoBI CYCGRG 1 cut(s) 471
Bsp143I GATC 3 cut(s) 88, 307, 533
Bsp68I TCGCGA 1 cut(s) 312
BspCNI CTCAG 2 cut(s) 183, 540
BspFNI CGCG 3 cut(s) 312, 422, 440
BspLI GGNNCC 2 cut(s) 658, 679
BsrBI CCGCTC 1 cut(s) 160
BsrFI RCCGGY 1 cut(s) 680
BssAI RCCGGY 1 cut(s) 680
BssECI CCNNGG 3 cut(s) 151, 414, 694
BssMI GATC 3 cut(s) 88, 307, 533
BssNI GRCGYC 1 cut(s) 428
Bst6I CTCTTC 1 cut(s) 558
BstACI GRCGYC 1 cut(s) 428
BstC8I GCNNGC 4 cut(s) 158, 502, 661, 682
BstDEI CTNAG 3 cut(s) 170, 548, 642
BstF5I GGATG 4 cut(s) 147, 185, 591, 600
BstFNI CGCG 3 cut(s) 312, 422, 440
BstKTI GATC 3 cut(s) 91, 310, 536
BstMAI GTCTC 1 cut(s) 816
BstMBI GATC 3 cut(s) 88, 307, 533
BstMWI GCNNNNNNNGC 7 cut(s) 25, 56, 388, 482, 488, 491, 497
BstNSI RCATGY 1 cut(s) 460
BstUI CGCG 3 cut(s) 312, 422, 440
BstV1I GCAGC 6 cut(s) 40, 49, 71, 472, 494, 498
BtsCI GGATG 4 cut(s) 147, 185, 591, 600
BtsI GCAGTG 1 cut(s) 783
BtsIMutI CAGTG 1 cut(s) 783
BtuMI TCGCGA 1 cut(s) 312
Cac8I GCNNGC 4 cut(s) 158, 502, 661, 682
Cfr10I RCCGGY 1 cut(s) 680
CseI GACGC 1 cut(s) 417
Csp6I GTAC 1 cut(s) 823
CviAII CATG 3 cut(s) 80, 201, 457
CviQI GTAC 1 cut(s) 823
DdeI CTNAG 3 cut(s) 170, 548, 642
DpnI GATC 3 cut(s) 90, 309, 535
DpnII GATC 3 cut(s) 88, 307, 533
DriI GACNNNNNGTC 1 cut(s) 607
Eam1104I CTCTTC 1 cut(s) 558
Eam1105I GACNNNNNGTC 1 cut(s) 607
EarI CTCTTC 1 cut(s) 558
EciI GGCGGA 1 cut(s) 371
Eco57I CTGAAG 2 cut(s) 404, 413
Eco88I CYCGRG 1 cut(s) 471
Esp3I CGTCTC 1 cut(s) 816
FaeI CATG 3 cut(s) 83, 204, 460
FaiI YATR 5 cut(s) 81, 202, 270, 332, 458
FaqI GGGAC 1 cut(s) 725
FatI CATG 3 cut(s) 79, 200, 456
FauI CCCGC 2 cut(s) 33, 43
FblI GTMKAC 1 cut(s) 105
FokI GGATG 4 cut(s) 134, 172, 598, 607
GsaI CCCAGC 2 cut(s) 19, 50
GsuI CTGGAG 2 cut(s) 383, 787
HapII CCGG 1 cut(s) 681
HgaI GACGC 1 cut(s) 417
Hin1I GRCGYC 1 cut(s) 428
Hin1II CATG 3 cut(s) 83, 204, 460
HincII GTYRAC 2 cut(s) 85, 106
HindII GTYRAC 2 cut(s) 85, 106
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 3 cut(s) 5, 404, 602
HpaI GTTAAC 1 cut(s) 85
HpaII CCGG 1 cut(s) 681
HphI GGTGA 3 cut(s) 83, 317, 531
Hpy166II GTNNAC 3 cut(s) 85, 106, 783
Hpy188I TCNGA 6 cut(s) 10, 173, 409, 549, 677, 698
Hpy188III TCNNGA 3 cut(s) 38, 114, 311
Hpy8I GTNNAC 3 cut(s) 85, 106, 783
Hpy99I CGWCG 2 cut(s) 146, 433
HpyAV CCTTC 2 cut(s) 367, 388
HpyCH4IV ACGT 2 cut(s) 507, 608
HpyCH4V TGCA 3 cut(s) 204, 514, 751
HpyF10VI GCNNNNNNNGC 7 cut(s) 25, 56, 388, 482, 488, 491, 497
HpyF3I CTNAG 3 cut(s) 170, 548, 642
HpySE526I ACGT 2 cut(s) 507, 608
Hsp92I GRCGYC 1 cut(s) 428
Hsp92II CATG 3 cut(s) 83, 204, 460
KroI GCCGGC 1 cut(s) 680
KroNI GCCGGC 1 cut(s) 682
KspAI GTTAAC 1 cut(s) 85
Kzo9I GATC 3 cut(s) 88, 307, 533
LmnI GCTCC 6 cut(s) 56, 358, 379, 442, 656, 677
LpnPI CCDG 9 cut(s) 29, 60, 347, 673, 684, 694, 731, 737, 751
Lsp1109I GCAGC 6 cut(s) 40, 49, 71, 472, 494, 498
LweI GCATC 1 cut(s) 378
MaeII ACGT 2 cut(s) 507, 608
MaeIII GTNAC 1 cut(s) 243
MalI GATC 3 cut(s) 90, 309, 535
MbiI CCGCTC 1 cut(s) 160
MboI GATC 3 cut(s) 88, 307, 533
MboII GAAGA 8 cut(s) 158, 284, 293, 397, 575, 724, 736, 739
MfeI CAATTG 1 cut(s) 818
MluCI AATT 3 cut(s) 119, 265, 818
MlyI GAGTC 2 cut(s) 413, 596
MmeI TCCRAC 2 cut(s) 387, 655
MroNI GCCGGC 1 cut(s) 680
MseI TTAA 1 cut(s) 84
MspA1I CMGCKG 3 cut(s) 50, 62, 485
MspI CCGG 1 cut(s) 681
MunI CAATTG 1 cut(s) 818
MvnI CGCG 3 cut(s) 312, 422, 440
MwoI GCNNNNNNNGC 7 cut(s) 25, 56, 388, 482, 488, 491, 497
NaeI GCCGGC 1 cut(s) 682
NdeII GATC 3 cut(s) 88, 307, 533
NgoMIV GCCGGC 1 cut(s) 680
NlaIII CATG 3 cut(s) 83, 204, 460
NlaIV GGNNCC 2 cut(s) 658, 679
NmeAIII GCCGAG 1 cut(s) 132
NmuCI GTSAC 1 cut(s) 243
NruI TCGCGA 1 cut(s) 312
NspI RCATGY 1 cut(s) 460
PaeR7I CTCGAG 1 cut(s) 471
PciI ACATGT 1 cut(s) 456
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PdiI GCCGGC 1 cut(s) 682
PfeI GAWTC 1 cut(s) 5
PflFI GACNNNGTC 1 cut(s) 671
PleI GAGTC 2 cut(s) 412, 596
PpsI GAGTC 2 cut(s) 412, 596
PscI ACATGT 1 cut(s) 456
PspFI CCCAGC 2 cut(s) 15, 46
PspN4I GGNNCC 2 cut(s) 658, 679
PspXI VCTCGAGB 1 cut(s) 471
PsyI GACNNNGTC 1 cut(s) 671
PvuII CAGCTG 2 cut(s) 62, 485
RruI TCGCGA 1 cut(s) 312
RsaI GTAC 1 cut(s) 824
RsaNI GTAC 1 cut(s) 823
SalI GTCGAC 1 cut(s) 104
SaqAI TTAA 1 cut(s) 84
Sau3AI GATC 3 cut(s) 88, 307, 533
SchI GAGTC 2 cut(s) 413, 596
SfaNI GCATC 1 cut(s) 378
Sfr274I CTCGAG 1 cut(s) 471
SlaI CTCGAG 1 cut(s) 471
SmlI CTYRAG 3 cut(s) 36, 350, 471
SmoI CTYRAG 3 cut(s) 36, 350, 471
Sse9I AATT 3 cut(s) 119, 265, 818
SspI AATATT 2 cut(s) 214, 624
TaiI ACGT 2 cut(s) 510, 611
TaqI TCGA 2 cut(s) 105, 472
TasI AATT 3 cut(s) 119, 265, 818
TatI WGTACW 1 cut(s) 822
TauI GCSGC 4 cut(s) 163, 262, 491, 494
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 1 cut(s) 84
Tru9I TTAA 1 cut(s) 84
TscAI CASTG 1 cut(s) 790
TseFI GTSAC 1 cut(s) 243
TseI GCWGC 6 cut(s) 28, 59, 62, 482, 485, 511
Tsp45I GTSAC 1 cut(s) 243
TspDTI ATGAA 3 cut(s) 174, 285, 737
TspGWI ACGGA 3 cut(s) 118, 231, 262
TspRI CASTG 1 cut(s) 790
Tth111I GACNNNGTC 1 cut(s) 671
XceI RCATGY 1 cut(s) 460
XhoI CTCGAG 1 cut(s) 471
XmiI GTMKAC 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.