Rroxscaffold_2G00078890

WEB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0015330
Physical Location & Seq
Forward (+)
2040220 .. 2040870
651 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00078890.1

Sequence Viewer

Length: 651 bp
ATGGAGACCCAAAATCTTCAAACCCTTCCAGTACCTCATGATTACCACGCCAACGTTGACAGTTCACGACCCTTCCGCTCCGTCAAGGAAGCTGTTGCCGTCTTTGGAGAGCGGCTTCTCCTTGGAGAGTTCTTCTCATCACCATCCCCTAAACCGCCGCAGCTGCTGCTATGTTACACCGAGCAAGAAGTAGAAACTTCTTCACCAAGGAACAAGTCTGTCATCTCATCACCAAGTACTAGTGCTGATCCACATAGTAAAGAAAGTGATAATGTTTTGGATAGTCTCAAGAAACTTGAGGCTGAGCTTGAGGAAACAAAGGTGGAGTTGAAGATGCTCAAGGAGAGAGAGTCCGAAACCGAGGTGGCCTTGGCAACCTTGAATGCAGAGCTTCACAAGAACATGTCCAAGTTGGCTCAGGCTGAGGCGGCCGCAGCAGCAAAAGCAGCGGCTAGTAGTGATCACCATGTTATCAGTTCGATGAGATCAAATTCGGAGGAAGCAGAGAAGAGTGTTAAGAAGGATCAGTGGTCGATGATAAGGATGGAGGACACTACTACTTTGGCTCAGATATTAAGCATTGGTTCTCAACATGAGAATCAATGTGGTTATTATGGGAGCGGAGGAAAAGAGAGAGGAATTATAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

216

Amino Acids

23.8

Weight (kDa)

5.45

Isoelectric Point (pI)

51.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015330)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G58070
fragaria_vesca FvH4_6g51930
malus_domestica MD08G1240500.v1.1 MD15G1433700.v1.1
prunus_persica Prupe.1G577200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0173411
rosa_laevigata RLG00000022187
rosa_multiflora Rmu_co8519037.1_g000002 Rmu_ssc0000282.1_g000013
rosa_roxburghii Rroxscaffold_2G00078890
rosa_rugosa Rorug02G0568100
rosa_samantha Rh2AG648600 Rh2BG659200 Rh2CG624500 Rh2DG674400
rosa_wichuraiana Rw2G053240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 644
AccBSI CCGCTC 3 cut(s) 78, 112, 621
AciI CCGC 8 cut(s) 76, 112, 155, 158, 428, 432, 449, 621
AclI AACGTT 1 cut(s) 54
AclWI GGATC 2 cut(s) 242, 531
AcoI YGGCCR 1 cut(s) 429
AcsI RAATTY 1 cut(s) 490
AfaI GTAC 2 cut(s) 33, 238
AflIII ACRYGT 1 cut(s) 402
AgsI TTSAA 3 cut(s) 20, 331, 382
AhlI ACTAGT 1 cut(s) 239
AluBI AGCT 4 cut(s) 92, 163, 307, 391
AluI AGCT 4 cut(s) 92, 163, 307, 391
Alw26I GTCTC 1 cut(s) 290
AlwI GGATC 2 cut(s) 242, 531
AlwNI CAGNNNCTG 1 cut(s) 166
AoxI GGCC 2 cut(s) 366, 429
ApeKI GCWGC 6 cut(s) 160, 163, 166, 434, 437, 446
ApoI RAATTY 1 cut(s) 490
AsuHPI GGTGA 4 cut(s) 132, 195, 222, 455
BbvCI CCTCAGC 1 cut(s) 423
BbvI GCAGC 6 cut(s) 150, 153, 172, 446, 449, 458
BccI CCATC 2 cut(s) 151, 538
BceAI ACGGC 1 cut(s) 83
BclI TGATCA 1 cut(s) 460
BcoDI GTCTC 1 cut(s) 290
BcuI ACTAGT 1 cut(s) 239
BfaI CTAG 2 cut(s) 240, 453
BlpI GCTNAGC 1 cut(s) 303
BmcAI AGTACT 1 cut(s) 238
BmsI GCATC 1 cut(s) 324
BplI GAGNNNNNCTC 2 cut(s) 119, 151
Bpu10I CCTNAGC 2 cut(s) 417, 423
Bpu1102I GCTNAGC 1 cut(s) 303
BpuEI CTTGAG 4 cut(s) 272, 317, 323, 329
BsaJI CCNNGG 4 cut(s) 121, 206, 360, 369
BsaXI ACNNNNNCTCC 2 cut(s) 335, 365
Bse1I ACTGG 1 cut(s) 29
BseDI CCNNGG 4 cut(s) 121, 206, 360, 369
BseGI GGATG 2 cut(s) 143, 549
BseMII CTCAG 4 cut(s) 294, 414, 431, 581
BseNI ACTGG 1 cut(s) 29
BseX3I CGGCCG 1 cut(s) 429
BseXI GCAGC 6 cut(s) 150, 153, 172, 446, 449, 458
Bsh1285I CGRYCG 1 cut(s) 432
BshFI GGCC 2 cut(s) 368, 431
BsiEI CGRYCG 1 cut(s) 432
BsmAI GTCTC 1 cut(s) 290
BsmI GAATGC 1 cut(s) 388
BsnI GGCC 2 cut(s) 368, 431
Bsp143I GATC 4 cut(s) 247, 460, 485, 523
Bsp1720I GCTNAGC 1 cut(s) 303
BspACI CCGC 8 cut(s) 76, 112, 155, 158, 428, 432, 449, 621
BspANI GGCC 2 cut(s) 368, 431
BspCNI CTCAG 4 cut(s) 295, 415, 430, 580
BspHI TCATGA 1 cut(s) 37
BspPI GGATC 2 cut(s) 242, 531
BsrBI CCGCTC 3 cut(s) 78, 112, 621
BsrI ACTGG 1 cut(s) 29
BssECI CCNNGG 4 cut(s) 121, 206, 360, 369
BssMI GATC 4 cut(s) 247, 460, 485, 523
BssT1I CCWWGG 3 cut(s) 121, 206, 369
Bst4CI ACNGT 1 cut(s) 62
Bst6I CTCTTC 1 cut(s) 503
BstAPI GCANNNNNTGC 1 cut(s) 166
BstDEI CTNAG 4 cut(s) 303, 417, 423, 567
BstF5I GGATG 2 cut(s) 143, 549
BstKTI GATC 4 cut(s) 250, 463, 488, 526
BstMAI GTCTC 1 cut(s) 290
BstMBI GATC 4 cut(s) 247, 460, 485, 523
BstMCI CGRYCG 1 cut(s) 432
BstMWI GCNNNNNNNGC 7 cut(s) 163, 166, 428, 434, 437, 443, 446
BstNSI RCATGY 1 cut(s) 406
BstV1I GCAGC 6 cut(s) 150, 153, 172, 446, 449, 458
BstZI CGGCCG 1 cut(s) 429
BsuRI GGCC 2 cut(s) 368, 431
BtsCI GGATG 2 cut(s) 143, 549
BtsIMutI CAGTG 1 cut(s) 533
CaiI CAGNNNCTG 1 cut(s) 166
CciI TCATGA 1 cut(s) 37
CciNI GCGGCCGC 1 cut(s) 429
Csp6I GTAC 2 cut(s) 32, 237
CviAII CATG 4 cut(s) 38, 403, 467, 593
CviQI GTAC 2 cut(s) 32, 237
DdeI CTNAG 4 cut(s) 303, 417, 423, 567
DpnI GATC 4 cut(s) 249, 462, 487, 525
DpnII GATC 4 cut(s) 247, 460, 485, 523
EaeI YGGCCR 1 cut(s) 429
EagI CGGCCG 1 cut(s) 429
Eam1104I CTCTTC 1 cut(s) 503
EarI CTCTTC 1 cut(s) 503
EclXI CGGCCG 1 cut(s) 429
Eco130I CCWWGG 3 cut(s) 121, 206, 369
Eco52I CGGCCG 1 cut(s) 429
EcoT14I CCWWGG 3 cut(s) 121, 206, 369
ErhI CCWWGG 3 cut(s) 121, 206, 369
FaeI CATG 4 cut(s) 41, 406, 470, 596
FaiI YATR 8 cut(s) 39, 172, 255, 404, 468, 594, 615, 644
FatI CATG 4 cut(s) 37, 402, 466, 592
FbaI TGATCA 1 cut(s) 460
FokI GGATG 2 cut(s) 130, 556
FspBI CTAG 2 cut(s) 240, 453
HaeIII GGCC 2 cut(s) 368, 431
Hin1II CATG 4 cut(s) 41, 406, 470, 596
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HinfI GANTC 2 cut(s) 350, 598
HphI GGTGA 4 cut(s) 132, 195, 222, 455
Hpy166II GTNNAC 2 cut(s) 58, 65
Hpy188I TCNGA 3 cut(s) 355, 496, 570
Hpy188III TCNNGA 3 cut(s) 38, 66, 289
Hpy8I GTNNAC 2 cut(s) 58, 65
HpyAV CCTTC 3 cut(s) 35, 82, 514
HpyCH4III ACNGT 1 cut(s) 62
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 1 cut(s) 386
HpyF10VI GCNNNNNNNGC 7 cut(s) 163, 166, 428, 434, 437, 443, 446
HpyF3I CTNAG 4 cut(s) 303, 417, 423, 567
HpySE526I ACGT 1 cut(s) 54
Hsp92II CATG 4 cut(s) 41, 406, 470, 596
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 4 cut(s) 247, 460, 485, 523
LmnI GCTCC 2 cut(s) 83, 618
LpnPI CCDG 2 cut(s) 42, 404
Lsp1109I GCAGC 6 cut(s) 150, 153, 172, 446, 449, 458
LweI GCATC 1 cut(s) 324
MaeI CTAG 2 cut(s) 240, 453
MaeII ACGT 1 cut(s) 54
MaeIII GTNAC 1 cut(s) 173
MalI GATC 4 cut(s) 249, 462, 487, 525
MbiI CCGCTC 3 cut(s) 78, 112, 621
MboI GATC 4 cut(s) 247, 460, 485, 523
MboII GAAGA 5 cut(s) 8, 124, 192, 343, 520
MluCI AATT 2 cut(s) 490, 639
MlyI GAGTC 1 cut(s) 359
MnlI CCTC 9 cut(s) 45, 292, 304, 355, 418, 490, 541, 617, 629
MseI TTAA 2 cut(s) 516, 575
MspA1I CMGCKG 2 cut(s) 163, 449
Mva1269I GAATGC 1 cut(s) 388
MwoI GCNNNNNNNGC 7 cut(s) 163, 166, 428, 434, 437, 443, 446
NdeII GATC 4 cut(s) 247, 460, 485, 523
NlaIII CATG 4 cut(s) 41, 406, 470, 596
NotI GCGGCCGC 1 cut(s) 429
NspI RCATGY 1 cut(s) 406
PagI TCATGA 1 cut(s) 37
PciI ACATGT 1 cut(s) 402
PctI GAATGC 1 cut(s) 388
PfeI GAWTC 1 cut(s) 598
PleI GAGTC 1 cut(s) 358
PpsI GAGTC 1 cut(s) 358
PscI ACATGT 1 cut(s) 402
PsiI TTATAA 1 cut(s) 644
Psp1406I AACGTT 1 cut(s) 54
PstNI CAGNNNCTG 1 cut(s) 166
PvuII CAGCTG 1 cut(s) 163
RsaI GTAC 2 cut(s) 33, 238
RsaNI GTAC 2 cut(s) 32, 237
SaqAI TTAA 2 cut(s) 516, 575
Sau3AI GATC 4 cut(s) 247, 460, 485, 523
ScaI AGTACT 1 cut(s) 238
SchI GAGTC 1 cut(s) 359
SetI ASST 9 cut(s) 37, 57, 94, 165, 309, 324, 366, 380, 393
SfaNI GCATC 1 cut(s) 324
SmlI CTYRAG 4 cut(s) 287, 296, 308, 338
SmoI CTYRAG 4 cut(s) 287, 296, 308, 338
SpeI ACTAGT 1 cut(s) 239
Sse9I AATT 2 cut(s) 490, 639
SsiI CCGC 8 cut(s) 76, 112, 155, 158, 428, 432, 449, 621
SspMI CTAG 2 cut(s) 240, 453
StyI CCWWGG 3 cut(s) 121, 206, 369
TaaI ACNGT 1 cut(s) 62
TaiI ACGT 1 cut(s) 57
TaqI TCGA 2 cut(s) 479, 533
TasI AATT 2 cut(s) 490, 639
TatI WGTACW 1 cut(s) 236
TauI GCSGC 5 cut(s) 115, 160, 431, 434, 452
TfiI GAWTC 1 cut(s) 598
Tru1I TTAA 2 cut(s) 516, 575
Tru9I TTAA 2 cut(s) 516, 575
TscAI CASTG 1 cut(s) 533
TseI GCWGC 6 cut(s) 160, 163, 166, 434, 437, 446
TspGWI ACGGA 1 cut(s) 70
TspRI CASTG 1 cut(s) 533
XapI RAATTY 1 cut(s) 490
XceI RCATGY 1 cut(s) 406
XspI CTAG 2 cut(s) 240, 453
ZrmI AGTACT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.