MD15G1433700.v1.1

WEB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015330
Physical Location & Seq
Reverse (-)
53388996 .. 53389898
903 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1433700.v1.1.491

Sequence Viewer

Length: 903 bp
ATGACCTTAATTCATGCACACCATTTCCTTCCATCTAGCACACACATATCCATGGAATCGGAAACCCAGCACTCCCTACAGCTTCCTGAAGAAAACCCAGCAGGTGGAGCAGCTGCCCTAACCCTACCACATGTTAACGATTACAACGCCAATGTCGACACTGCCCGACCCTTCCGCTCCGTCAAAGAAGCCGTAGCGATCTTTGACGAGCGCCTTCTTCTCGGAGACATGTTTTCATCCCCTAAACCCCTATACACCAATAATATTGTCCAAAGTAGCCCTACTACCACTCCATCATGGAAGTTTTCGCCGCCGAACTATAAAGAAAGCGAAGAAGTAAGAGTTAGTAGTTATGGTGATCGCGAGGAGAAAGTAGCAGTTATGGACACTCTTAAGAAGCTCGAGGCGGAGCTCGAGGAAACAAAGGTGGAGCTGAAGTTGCTGAAGGAGAGGGAGTCAGAAACGGAGGTGGCACTGGCGTCGTTGAACGCGGAGCTCCACAAGAACATGTCCAAGTTGGCTCGAGCGGAGGCGGATGCGGCAGCAAAGGCGAGTAACGTGCTGCATGTGTCAACAACTACTACATCATCATCACCAAATATTACTACTATGGGAGATAATGTTGAAGAGCGGAATAAGGGGGAGTGGATGATAAGGATGGAGAACTCTACGTCTTTGGCTCAAGTATTGACCATTGGTACTACTGAGAAACAAGGAGGAGCCGGCTACCACCATGTCGGAGCCGGCTACTACGACTATGTCGGAAGGAACAGAGAGAAGAAAACAATGAAGAAAAAGCCTATTGTCCCTCTTGTGCAAGATTTATTGTCGTGGAAAAAAGGGTCTAAATATTCCAGTTCTCTTTGCAACCCTTTTTATTCGTCTACGCAATTGTACTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

301

Amino Acids

33.36

Weight (kDa)

6.23

Isoelectric Point (pI)

47.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015330)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G58070
fragaria_vesca FvH4_6g51930
malus_domestica MD08G1240500.v1.1 MD15G1433700.v1.1
prunus_persica Prupe.1G577200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0173411
rosa_laevigata RLG00000022187
rosa_multiflora Rmu_co8519037.1_g000002 Rmu_ssc0000282.1_g000013
rosa_roxburghii Rroxscaffold_2G00078890
rosa_rugosa Rorug02G0568100
rosa_samantha Rh2AG648600 Rh2BG659200 Rh2CG624500 Rh2DG674400
rosa_wichuraiana Rw2G053240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 92
Acc36I ACCTGC 1 cut(s) 92
AccB7I CCANNNNNTGG 1 cut(s) 104
AccBSI CCGCTC 3 cut(s) 177, 527, 631
AccI GTMKAC 2 cut(s) 156, 884
AccII CGCG 2 cut(s) 363, 491
AciI CCGC 8 cut(s) 175, 311, 407, 491, 527, 533, 539, 631
AcuI CTGAAG 3 cut(s) 108, 455, 464
AcyI GRCGYC 1 cut(s) 479
AfaI GTAC 2 cut(s) 700, 896
AfiI CCNNNNNNNGG 1 cut(s) 104
AflII CTTAAG 1 cut(s) 392
AflIII ACRYGT 3 cut(s) 130, 228, 507
AgsI TTSAA 2 cut(s) 487, 626
AluBI AGCT 6 cut(s) 82, 113, 400, 412, 433, 496
AluI AGCT 6 cut(s) 82, 113, 400, 412, 433, 496
Alw21I GWGCWC 2 cut(s) 414, 498
Alw26I GTCTC 1 cut(s) 219
Ama87I CYCGRG 3 cut(s) 401, 413, 522
ApeKI GCWGC 4 cut(s) 110, 113, 542, 562
AspLEI GCGC 1 cut(s) 213
AsuHPI GGTGA 2 cut(s) 368, 585
AvaI CYCGRG 3 cut(s) 401, 413, 522
BanII GRGCYC 2 cut(s) 414, 498
Bbv12I GWGCWC 2 cut(s) 414, 498
BbvI GCAGC 4 cut(s) 100, 122, 549, 554
BccI CCATC 3 cut(s) 40, 301, 652
BceAI ACGGC 1 cut(s) 176
BcgI CGANNNNNNTGC 4 cut(s) 462, 496, 541, 575
BcoDI GTCTC 1 cut(s) 219
BfaI CTAG 1 cut(s) 36
BfmI CTRYAG 1 cut(s) 77
BfoI RGCGCY 1 cut(s) 214
BfrI CTTAAG 1 cut(s) 392
BfuAI ACCTGC 1 cut(s) 92
BisI GCNGC 6 cut(s) 111, 114, 311, 540, 543, 563
BlsI GCNGC 6 cut(s) 112, 115, 312, 541, 544, 564
BmeT110I CYCGRG 3 cut(s) 401, 413, 522
BmiI GGNNCC 2 cut(s) 721, 742
BmsI GCATC 1 cut(s) 526
BpuEI CTTGAG 1 cut(s) 666
BsaHI GRCGYC 1 cut(s) 479
BsaJI CCNNGG 1 cut(s) 51
BsaXI ACNNNNNCTCC 8 cut(s) 274, 304, 422, 440, 452, 470, 606, 636
Bsc4I CCNNNNNNNGG 1 cut(s) 104
Bse118I RCCGGY 2 cut(s) 722, 743
Bse1I ACTGG 2 cut(s) 480, 855
BseDI CCNNGG 1 cut(s) 51
BseGI GGATG 4 cut(s) 236, 541, 654, 663
BseLI CCNNNNNNNGG 1 cut(s) 104
BseMII CTCAG 1 cut(s) 696
BseNI ACTGG 2 cut(s) 480, 855
BseRI GAGGAG 2 cut(s) 380, 732
BseXI GCAGC 4 cut(s) 100, 122, 549, 554
BseYI CCCAGC 2 cut(s) 66, 97
Bsh1236I CGCG 2 cut(s) 363, 491
BsiHKAI GWGCWC 2 cut(s) 414, 498
BsiHKCI CYCGRG 3 cut(s) 401, 413, 522
BsiSI CCGG 2 cut(s) 723, 744
BslFI GGGAC 1 cut(s) 791
BslI CCNNNNNNNGG 1 cut(s) 104
BsmAI GTCTC 1 cut(s) 219
BsmFI GGGAC 1 cut(s) 791
BsoBI CYCGRG 3 cut(s) 401, 413, 522
Bsp1286I GDGCHC 2 cut(s) 414, 498
Bsp143I GATC 2 cut(s) 198, 358
Bsp19I CCATGG 1 cut(s) 51
Bsp68I TCGCGA 1 cut(s) 363
BspACI CCGC 8 cut(s) 175, 311, 407, 491, 527, 533, 539, 631
BspCNI CTCAG 1 cut(s) 697
BspFNI CGCG 2 cut(s) 363, 491
BspLI GGNNCC 2 cut(s) 721, 742
BspMI ACCTGC 1 cut(s) 92
BspQI GCTCTTC 1 cut(s) 621
BspTI CTTAAG 1 cut(s) 392
BsrBI CCGCTC 3 cut(s) 177, 527, 631
BsrFI RCCGGY 2 cut(s) 722, 743
BsrI ACTGG 2 cut(s) 480, 855
BssAI RCCGGY 2 cut(s) 722, 743
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 2 cut(s) 198, 358
BssNI GRCGYC 1 cut(s) 479
BssT1I CCWWGG 1 cut(s) 51
Bst6I CTCTTC 1 cut(s) 621
BstACI GRCGYC 1 cut(s) 479
BstAFI CTTAAG 1 cut(s) 392
BstC8I GCNNGC 2 cut(s) 724, 745
BstDEI CTNAG 1 cut(s) 705
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 4 cut(s) 236, 541, 654, 663
BstFNI CGCG 2 cut(s) 363, 491
BstH2I RGCGCY 1 cut(s) 214
BstHHI GCGC 1 cut(s) 213
BstKTI GATC 2 cut(s) 201, 361
BstMAI GTCTC 1 cut(s) 219
BstMBI GATC 2 cut(s) 198, 358
BstMWI GCNNNNNNNGC 4 cut(s) 107, 439, 539, 548
BstNSI RCATGY 4 cut(s) 134, 232, 511, 569
BstSFI CTRYAG 1 cut(s) 77
BstUI CGCG 2 cut(s) 363, 491
BstV1I GCAGC 4 cut(s) 100, 122, 549, 554
BtgI CCRYGG 1 cut(s) 51
BtsCI GGATG 4 cut(s) 236, 541, 654, 663
BtsI GCAGTG 1 cut(s) 159
BtsIMutI CAGTG 2 cut(s) 159, 473
BtuMI TCGCGA 1 cut(s) 363
BveI ACCTGC 1 cut(s) 92
Cac8I GCNNGC 2 cut(s) 724, 745
CfoI GCGC 1 cut(s) 213
Cfr10I RCCGGY 2 cut(s) 722, 743
CseI GACGC 1 cut(s) 468
Csp6I GTAC 2 cut(s) 699, 895
CviAII CATG 8 cut(s) 14, 52, 131, 229, 297, 508, 566, 734
CviQI GTAC 2 cut(s) 699, 895
DdeI CTNAG 1 cut(s) 705
DpnI GATC 2 cut(s) 200, 360
DpnII GATC 2 cut(s) 198, 358
Eam1104I CTCTTC 1 cut(s) 621
EarI CTCTTC 1 cut(s) 621
EciI GGCGGA 2 cut(s) 422, 548
Ecl136II GAGCTC 2 cut(s) 412, 496
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 2 cut(s) 414, 498
Eco53kI GAGCTC 2 cut(s) 412, 496
Eco57I CTGAAG 3 cut(s) 108, 455, 464
Eco88I CYCGRG 3 cut(s) 401, 413, 522
EcoICRI GAGCTC 2 cut(s) 412, 496
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 2 cut(s) 414, 498
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 8 cut(s) 17, 55, 134, 232, 300, 511, 569, 737
FaqI GGGAC 1 cut(s) 791
FatI CATG 8 cut(s) 13, 51, 130, 228, 296, 507, 565, 733
FblI GTMKAC 2 cut(s) 156, 884
Fnu4HI GCNGC 6 cut(s) 111, 114, 311, 540, 543, 563
FokI GGATG 4 cut(s) 223, 548, 661, 670
FriOI GRGCYC 2 cut(s) 414, 498
Fsp4HI GCNGC 6 cut(s) 111, 114, 311, 540, 543, 563
FspBI CTAG 1 cut(s) 36
GlaI GCGC 1 cut(s) 212
GluI GCNGC 6 cut(s) 111, 114, 311, 540, 543, 563
GsaI CCCAGC 2 cut(s) 70, 101
HaeII RGCGCY 1 cut(s) 214
HapII CCGG 2 cut(s) 723, 744
HgaI GACGC 1 cut(s) 468
HhaI GCGC 1 cut(s) 213
Hin1I GRCGYC 1 cut(s) 479
Hin1II CATG 8 cut(s) 17, 55, 134, 232, 300, 511, 569, 737
Hin6I GCGC 1 cut(s) 211
HinP1I GCGC 1 cut(s) 211
HincII GTYRAC 3 cut(s) 136, 157, 573
HindII GTYRAC 3 cut(s) 136, 157, 573
HinfI GANTC 2 cut(s) 56, 455
HpaI GTTAAC 1 cut(s) 136
HpaII CCGG 2 cut(s) 723, 744
HphI GGTGA 2 cut(s) 368, 585
Hpy166II GTNNAC 4 cut(s) 136, 157, 573, 885
Hpy188I TCNGA 5 cut(s) 61, 224, 460, 740, 764
Hpy188III TCNNGA 2 cut(s) 86, 362
Hpy8I GTNNAC 4 cut(s) 136, 157, 573, 885
Hpy99I CGWCG 1 cut(s) 484
HpyAV CCTTC 5 cut(s) 38, 181, 224, 439, 759
HpyCH4IV ACGT 2 cut(s) 558, 671
HpyCH4V TGCA 4 cut(s) 17, 565, 817, 867
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 439, 539, 548
HpyF3I CTNAG 1 cut(s) 705
HpySE526I ACGT 2 cut(s) 558, 671
Hsp92I GRCGYC 1 cut(s) 479
Hsp92II CATG 8 cut(s) 17, 55, 134, 232, 300, 511, 569, 737
HspAI GCGC 1 cut(s) 211
KroI GCCGGC 2 cut(s) 722, 743
KroNI GCCGGC 2 cut(s) 724, 745
KspAI GTTAAC 1 cut(s) 136
Kzo9I GATC 2 cut(s) 198, 358
LguI GCTCTTC 1 cut(s) 621
LmnI GCTCC 8 cut(s) 107, 182, 409, 430, 493, 501, 719, 740
LpnPI CCDG 8 cut(s) 80, 87, 99, 111, 461, 736, 757, 868
Lsp1109I GCAGC 4 cut(s) 100, 122, 549, 554
LweI GCATC 1 cut(s) 526
MaeI CTAG 1 cut(s) 36
MaeII ACGT 2 cut(s) 558, 671
MaeIII GTNAC 1 cut(s) 554
MalI GATC 2 cut(s) 200, 360
MbiI CCGCTC 3 cut(s) 177, 527, 631
MboI GATC 2 cut(s) 198, 358
MboII GAAGA 6 cut(s) 101, 209, 344, 638, 790, 802
MfeI CAATTG 1 cut(s) 890
MhlI GDGCHC 2 cut(s) 414, 498
MluCI AATT 2 cut(s) 9, 890
MlyI GAGTC 1 cut(s) 464
MmeI TCCRAC 2 cut(s) 718, 742
MnlI CCTC 8 cut(s) 358, 397, 409, 444, 460, 523, 710, 819
MroNI GCCGGC 2 cut(s) 722, 743
MseI TTAA 3 cut(s) 8, 135, 393
MslI CAYNNNNRTG 1 cut(s) 50
MspA1I CMGCKG 1 cut(s) 113
MspCI CTTAAG 1 cut(s) 392
MspI CCGG 2 cut(s) 723, 744
MunI CAATTG 1 cut(s) 890
MvnI CGCG 2 cut(s) 363, 491
MwoI GCNNNNNNNGC 4 cut(s) 107, 439, 539, 548
NaeI GCCGGC 2 cut(s) 724, 745
NcoI CCATGG 1 cut(s) 51
NdeII GATC 2 cut(s) 198, 358
NgoMIV GCCGGC 2 cut(s) 722, 743
NlaIII CATG 8 cut(s) 17, 55, 134, 232, 300, 511, 569, 737
NlaIV GGNNCC 2 cut(s) 721, 742
NruI TCGCGA 1 cut(s) 363
NspI RCATGY 4 cut(s) 134, 232, 511, 569
PaeR7I CTCGAG 3 cut(s) 401, 413, 522
PaqCI CACCTGC 1 cut(s) 92
PciI ACATGT 3 cut(s) 130, 228, 507
PciSI GCTCTTC 1 cut(s) 621
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PdiI GCCGGC 2 cut(s) 724, 745
PfeI GAWTC 1 cut(s) 56
PflFI GACNNNGTC 1 cut(s) 758
PflMI CCANNNNNTGG 1 cut(s) 104
PkrI GCNGC 6 cut(s) 112, 115, 312, 541, 544, 564
PleI GAGTC 1 cut(s) 463
PpsI GAGTC 1 cut(s) 463
PscI ACATGT 3 cut(s) 130, 228, 507
Psp124BI GAGCTC 2 cut(s) 414, 498
PspFI CCCAGC 2 cut(s) 66, 97
PspN4I GGNNCC 2 cut(s) 721, 742
PspXI VCTCGAGB 3 cut(s) 401, 413, 522
PsyI GACNNNGTC 1 cut(s) 758
PvuII CAGCTG 1 cut(s) 113
RruI TCGCGA 1 cut(s) 363
RsaI GTAC 2 cut(s) 700, 896
RsaNI GTAC 2 cut(s) 699, 895
RseI CAYNNNNRTG 1 cut(s) 50
SacI GAGCTC 2 cut(s) 414, 498
SalI GTCGAC 1 cut(s) 155
SapI GCTCTTC 1 cut(s) 621
SaqAI TTAA 3 cut(s) 8, 135, 393
SatI GCNGC 6 cut(s) 111, 114, 311, 540, 543, 563
Sau3AI GATC 2 cut(s) 198, 358
SchI GAGTC 1 cut(s) 464
SduI GDGCHC 2 cut(s) 414, 498
SfaNI GCATC 1 cut(s) 526
SfcI CTRYAG 1 cut(s) 77
Sfr274I CTCGAG 3 cut(s) 401, 413, 522
SlaI CTCGAG 3 cut(s) 401, 413, 522
SmiMI CAYNNNNRTG 1 cut(s) 50
SmlI CTYRAG 5 cut(s) 392, 401, 413, 522, 681
SmoI CTYRAG 5 cut(s) 392, 401, 413, 522, 681
Sse9I AATT 2 cut(s) 9, 890
SsiI CCGC 8 cut(s) 175, 311, 407, 491, 527, 533, 539, 631
SspI AATATT 3 cut(s) 265, 601, 851
SspMI CTAG 1 cut(s) 36
SstI GAGCTC 2 cut(s) 414, 498
StyI CCWWGG 1 cut(s) 51
TaiI ACGT 2 cut(s) 561, 674
TaqI TCGA 4 cut(s) 156, 402, 414, 523
TasI AATT 2 cut(s) 9, 890
TatI WGTACW 1 cut(s) 894
TauI GCSGC 2 cut(s) 313, 542
TfiI GAWTC 1 cut(s) 56
Tru1I TTAA 3 cut(s) 8, 135, 393
Tru9I TTAA 3 cut(s) 8, 135, 393
TscAI CASTG 2 cut(s) 166, 480
TseI GCWGC 4 cut(s) 110, 113, 542, 562
TspDTI ATGAA 2 cut(s) 225, 803
TspGWI ACGGA 2 cut(s) 169, 479
TspRI CASTG 2 cut(s) 166, 480
Tth111I GACNNNGTC 1 cut(s) 758
Van91I CCANNNNNTGG 1 cut(s) 104
Vha464I CTTAAG 1 cut(s) 392
XceI RCATGY 4 cut(s) 134, 232, 511, 569
XhoI CTCGAG 3 cut(s) 401, 413, 522
XmiI GTMKAC 2 cut(s) 156, 884
XspI CTAG 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.