Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0014928

Physical Location & Seq

Genomic Coordinates Reverse (-)

30943261 .. 30952177

Gene Structure 8917 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1244400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 606 bp

ATGAGCAACGAATACGATTATTTGTTCAAGCTTCTGCTAATTGGGGACTCGTCCGTTGGAAAATCTTGTTTGCTTCTCAGATTCGCTGATGATTCCTATGTTGACAGCTACATAAGCACCATTGGAGTTGATTTTAAAATCAGAACAGTGGAGCTGGATGGCAAGACGGTCAAGCTGCAGATTTGGGACACTGCTGGACAGGAGCGATTCAGGACTATAACAAGCAGTTACTACCGAGGAGCACATGGGATAATTATTGTGTATGATGTTACTGAGATGGAGAGCTTCAATAACGTGAAGCAGTGGCTGAATGAGATTGACAGATATGCAAGTGACAGTGTGTGCAAGCTTCTAGTTGGTAATAAATGTGATTTAGTTGAGACCAAGGTTGTCGATACACAAACTGCCAAGGCTTTTGCTGATGAGCTTGGGATCCCTTTCCTCGAGACAAGTGCTAAAGACTCGATCAATGTTGAGCAGGCTTTCTTAACAATGGCAGCCGAGATAAAGAAAAAAATGGGAAGCCAACCAACTGGTAACAGGTCGAGCGGAACTGTTGAAATGAAGAGCCAGCCAATTCAGCAGAACAGCAGTTGTTGCGGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006888 GO:0008092 GO:0008150 GO:0012505 GO:0016020 GO:0016192 GO:0017022 GO:0030742 GO:0031090 GO:0031984 GO:0032029 GO:0032036 GO:0032588 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048193 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0071944 GO:0080115 GO:0098588 GO:0098791

KEGG Pathways

Metabolic & Signaling

ko05134 map05134

Pfam Domains

Protein Families

Ras

Protein Analysis

202

Amino Acids

22.39

Weight (kDa)

5.01

Isoelectric Point (pI)

32.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arf	PF00025	9 - 132	3.9e-16	ADP-ribosylation factor family
Ras	PF00071	10 - 170	1.8e-67	Ras family
Roc	PF08477	10 - 124	8.1e-37	Ras of Complex, Roc, domain of DAPkinase
GTP_EFTU	PF00009	43 - 166	3.7e-06	Elongation factor Tu GTP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014928)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11730
fragaria_vesca	FvH4_5g28100
malus_domestica	MD08G1244400.v1.1 MD15G1439100.v1.1
prunus_persica	Prupe.1G581900_v2.0.a1
pyrus_communis	pycom08g21200 pycom15g38720
rosa_chinensis	RchiOBHm_Chr7g0220361
rosa_laevigata	RLG00000002226
rosa_multiflora	Rmu_sc0000099.1_g000014
rosa_roxburghii	Rroxscaffold_3G00239450
rosa_rugosa	Rorug07G0193200
rosa_samantha	Rh7AG334600 Rh7CG352300 Rh7DG331300
rosa_wichuraiana	Rw7G028250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	549
AciI	CCGC	2 cut(s)	549, 600
AclWI	GGATC	2 cut(s)	427, 440
AgsI	TTSAA	3 cut(s)	28, 289, 560
AluBI	AGCT	7 cut(s)	31, 108, 154, 175, 285, 349, 427
AluI	AGCT	7 cut(s)	31, 108, 154, 175, 285, 349, 427
Alw21I	GWGCWC	1 cut(s)	244
Alw26I	GTCTC	2 cut(s)	374, 440
AlwI	GGATC	2 cut(s)	427, 440
AlwNI	CAGNNNCTG	1 cut(s)	307
Ama87I	CYCGRG	1 cut(s)	443
ApeKI	GCWGC	2 cut(s)	175, 497
AvaI	CYCGRG	1 cut(s)	443
BamHI	GGATCC	1 cut(s)	432
Bbv12I	GWGCWC	1 cut(s)	244
BbvI	GCAGC	2 cut(s)	162, 509
BccI	CCATC	2 cut(s)	152, 271
BcgI	CGANNNNNNTGC	2 cut(s)	434, 468
BcoDI	GTCTC	2 cut(s)	374, 440
BfaI	CTAG	1 cut(s)	353
BfmI	CTRYAG	1 cut(s)	176
BisI	GCNGC	2 cut(s)	176, 498
BlsI	GCNGC	2 cut(s)	177, 499
BmeT110I	CYCGRG	1 cut(s)	443
BmiI	GGNNCC	1 cut(s)	434
BsaI	GGTCTC	1 cut(s)	374
BsaJI	CCNNGG	3 cut(s)	235, 384, 408
Bse1I	ACTGG	1 cut(s)	538
BseDI	CCNNGG	3 cut(s)	235, 384, 408
BseGI	GGATG	1 cut(s)	163
BseMII	CTCAG	2 cut(s)	91, 264
BseNI	ACTGG	1 cut(s)	538
BseRI	GAGGAG	1 cut(s)	252
BseXI	GCAGC	2 cut(s)	162, 509
BsiHKAI	GWGCWC	1 cut(s)	244
BsiHKCI	CYCGRG	1 cut(s)	443
BslFI	GGGAC	2 cut(s)	59, 200
BsmAI	GTCTC	2 cut(s)	374, 440
BsmFI	GGGAC	2 cut(s)	59, 200
Bso31I	GGTCTC	1 cut(s)	374
BsoBI	CYCGRG	1 cut(s)	443
Bsp1286I	GDGCHC	1 cut(s)	244
Bsp143I	GATC	2 cut(s)	432, 465
BspACI	CCGC	2 cut(s)	549, 600
BspCNI	CTCAG	2 cut(s)	90, 265
BspLI	GGNNCC	1 cut(s)	434
BspMAI	CTGCAG	1 cut(s)	180
BspPI	GGATC	2 cut(s)	427, 440
BspQI	GCTCTTC	1 cut(s)	560
BspTNI	GGTCTC	1 cut(s)	374
BsrBI	CCGCTC	1 cut(s)	549
BsrI	ACTGG	1 cut(s)	538
BssECI	CCNNGG	3 cut(s)	235, 384, 408
BssMI	GATC	2 cut(s)	432, 465
BssT1I	CCWWGG	2 cut(s)	384, 408
Bst4CI	ACNGT	4 cut(s)	148, 169, 338, 556
Bst6I	CTCTTC	1 cut(s)	560
BstAPI	GCANNNNNTGC	1 cut(s)	597
BstC8I	GCNNGC	3 cut(s)	347, 480, 572
BstDEI	CTNAG	2 cut(s)	77, 273
BstF5I	GGATG	1 cut(s)	163
BstKTI	GATC	2 cut(s)	435, 468
BstMAI	GTCTC	2 cut(s)	374, 440
BstMBI	GATC	2 cut(s)	432, 465
BstMWI	GCNNNNNNNGC	3 cut(s)	114, 580, 597
BstSFI	CTRYAG	1 cut(s)	176
BstV1I	GCAGC	2 cut(s)	162, 509
BstX2I	RGATCY	1 cut(s)	432
BstXI	CCANNNNNNTGG	1 cut(s)	533
BstYI	RGATCY	1 cut(s)	432
BtsCI	GGATG	1 cut(s)	163
BtsI	GCAGTG	2 cut(s)	189, 308
BtsIMutI	CAGTG	4 cut(s)	153, 189, 308, 343
Cac8I	GCNNGC	3 cut(s)	347, 480, 572
CaiI	CAGNNNCTG	1 cut(s)	307
CviAII	CATG	1 cut(s)	245
DdeI	CTNAG	2 cut(s)	77, 273
DpnI	GATC	2 cut(s)	434, 467
DpnII	GATC	2 cut(s)	432, 465
DraI	TTTAAA	1 cut(s)	136
Eam1104I	CTCTTC	1 cut(s)	560
EarI	CTCTTC	1 cut(s)	560
Eco130I	CCWWGG	2 cut(s)	384, 408
Eco31I	GGTCTC	1 cut(s)	374
Eco88I	CYCGRG	1 cut(s)	443
EcoT14I	CCWWGG	2 cut(s)	384, 408
ErhI	CCWWGG	2 cut(s)	384, 408
FaeI	CATG	1 cut(s)	248
FaiI	YATR	6 cut(s)	99, 113, 218, 246, 264, 327
FaqI	GGGAC	2 cut(s)	59, 200
FatI	CATG	1 cut(s)	244
Fnu4HI	GCNGC	2 cut(s)	176, 498
FokI	GGATG	1 cut(s)	170
Fsp4HI	GCNGC	2 cut(s)	176, 498
FspBI	CTAG	1 cut(s)	353
GluI	GCNGC	2 cut(s)	176, 498
Hin1II	CATG	1 cut(s)	248
HincII	GTYRAC	1 cut(s)	103
HindII	GTYRAC	1 cut(s)	103
HindIII	AAGCTT	2 cut(s)	29, 347
HinfI	GANTC	5 cut(s)	47, 81, 92, 207, 461
Hpy166II	GTNNAC	1 cut(s)	103
Hpy188I	TCNGA	2 cut(s)	80, 143
Hpy188III	TCNNGA	2 cut(s)	211, 445
Hpy8I	GTNNAC	1 cut(s)	103
HpyCH4III	ACNGT	4 cut(s)	148, 169, 338, 556
HpyCH4IV	ACGT	1 cut(s)	294
HpyCH4V	TGCA	3 cut(s)	178, 329, 345
HpyF10VI	GCNNNNNNNGC	3 cut(s)	114, 580, 597
HpyF3I	CTNAG	2 cut(s)	77, 273
HpySE526I	ACGT	1 cut(s)	294
Hsp92II	CATG	1 cut(s)	248
Kzo9I	GATC	2 cut(s)	432, 465
LguI	GCTCTTC	1 cut(s)	560
LmnI	GCTCC	3 cut(s)	151, 202, 239
LpnPI	CCDG	8 cut(s)	140, 180, 185, 196, 464, 519, 526, 584
Lsp1109I	GCAGC	2 cut(s)	162, 509
MaeI	CTAG	1 cut(s)	353
MaeII	ACGT	1 cut(s)	294
MaeIII	GTNAC	4 cut(s)	227, 268, 332, 536
MalI	GATC	2 cut(s)	434, 467
MbiI	CCGCTC	1 cut(s)	549
MboI	GATC	2 cut(s)	432, 465
MboII	GAAGA	1 cut(s)	577
MflI	RGATCY	1 cut(s)	432
MhlI	GDGCHC	1 cut(s)	244
MluCI	AATT	3 cut(s)	39, 252, 576
MlyI	GAGTC	2 cut(s)	41, 455
MmeI	TCCRAC	1 cut(s)	37
MnlI	CCTC	2 cut(s)	230, 452
MseI	TTAA	2 cut(s)	135, 488
MwoI	GCNNNNNNNGC	3 cut(s)	114, 580, 597
NdeII	GATC	2 cut(s)	432, 465
NlaIII	CATG	1 cut(s)	248
NlaIV	GGNNCC	1 cut(s)	434
NmeAIII	GCCGAG	1 cut(s)	526
NmuCI	GTSAC	1 cut(s)	332
PaeR7I	CTCGAG	1 cut(s)	443
PciSI	GCTCTTC	1 cut(s)	560
PfeI	GAWTC	3 cut(s)	81, 92, 207
PkrI	GCNGC	2 cut(s)	177, 499
PleI	GAGTC	2 cut(s)	41, 455
PpsI	GAGTC	2 cut(s)	41, 455
PspN4I	GGNNCC	1 cut(s)	434
PstI	CTGCAG	1 cut(s)	180
PstNI	CAGNNNCTG	1 cut(s)	307
PsuI	RGATCY	1 cut(s)	432
SapI	GCTCTTC	1 cut(s)	560
SaqAI	TTAA	2 cut(s)	135, 488
SatI	GCNGC	2 cut(s)	176, 498
Sau3AI	GATC	2 cut(s)	432, 465
SchI	GAGTC	2 cut(s)	41, 455
SduI	GDGCHC	1 cut(s)	244
SfcI	CTRYAG	1 cut(s)	176
Sfr274I	CTCGAG	1 cut(s)	443
SlaI	CTCGAG	1 cut(s)	443
SmlI	CTYRAG	1 cut(s)	443
SmoI	CTYRAG	1 cut(s)	443
Sse9I	AATT	3 cut(s)	39, 252, 576
SsiI	CCGC	2 cut(s)	549, 600
SspMI	CTAG	1 cut(s)	353
StyI	CCWWGG	2 cut(s)	384, 408
TaaI	ACNGT	4 cut(s)	148, 169, 338, 556
TaiI	ACGT	1 cut(s)	297
TaqI	TCGA	4 cut(s)	393, 444, 464, 545
TasI	AATT	3 cut(s)	39, 252, 576
TfiI	GAWTC	3 cut(s)	81, 92, 207
Tru1I	TTAA	2 cut(s)	135, 488
Tru9I	TTAA	2 cut(s)	135, 488
TscAI	CASTG	4 cut(s)	153, 196, 308, 343
TseFI	GTSAC	1 cut(s)	332
TseI	GCWGC	2 cut(s)	175, 497
Tsp45I	GTSAC	1 cut(s)	332
TspDTI	ATGAA	1 cut(s)	578
TspGWI	ACGGA	1 cut(s)	43
TspRI	CASTG	4 cut(s)	153, 196, 308, 343
XhoI	CTCGAG	1 cut(s)	443
XspI	CTAG	1 cut(s)	353

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.