RchiOBHm_Chr7g0220361

Ras-related protein RABD1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0014928
Physical Location & Seq
Forward (+)
39253037 .. 39258170
5134 bp
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UTR
Exon/CDS
Intron
PRQ19726

Sequence Viewer

Length: 606 bp
ATGAGCAACGAATATGATTACTTGTTCAAGCTCTTGCTAATTGGGGACTCCTCCGTCGGAAAATCGTGTTTGCTTGTCAGATTCGCTGACGATTCCTATGTGGACAGCTACGTCAGCACCATTGGAGTTGATTTTAAAATCAGGACTGTGGAGCTGGATGGGAAGACTGTCAAGCTGCAGATTTGGGATACTGCTGGACAAGAGCGATTCAGGACCATAACAAGCAGTTACTACCGAGGAGCCCATGGGATAATTATTGTCTATGATGTTACTGAGATGGAGAGCTTCAATAATGTCAAGCAGTGGCTGAATGAGATTGACAGATATGCAAATGAAAGTGTGTGCAAGCTTTTAGTGGGAAATAAATGTGATTTAGTTGAGAACAAGGTTGTTGACACGCAAACAGGAAAGGCTTTAGCTGATGAGCTTGGTATCCCTTTCCTCGAGACAAGTGCTAAAGACGCTATCAATGTTGAGCAGGCTTTCTTGACCATGGCTGGCGAGATAAAGAAAAAAATGGGCAACCAACCAACTGCTAACAAGTCAAGTGAAACTGTTCAAATGAAGGGGAATCCCATTCAGCAGAATAGCAATTGTTGTGGTTAG

Protein Analysis

201

Amino Acids

22.41

Weight (kDa)

5.02

Isoelectric Point (pI)

24.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 9 - 130 1.9e-16 ADP-ribosylation factor family
Ras PF00071 10 - 170 2.9e-67 Ras family
Roc PF08477 10 - 124 6.9e-37 Ras of Complex, Roc, domain of DAPkinase
Gtr1_RagA PF04670 10 - 130 5.9e-06 Gtr1/RagA G protein conserved region
GTP_EFTU PF00009 43 - 164 6.2e-06 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014928)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11730
fragaria_vesca FvH4_5g28100
malus_domestica MD08G1244400.v1.1 MD15G1439100.v1.1
prunus_persica Prupe.1G581900_v2.0.a1
pyrus_communis pycom08g21200 pycom15g38720
rosa_chinensis RchiOBHm_Chr7g0220361
rosa_laevigata RLG00000002226
rosa_multiflora Rmu_sc0000099.1_g000014
rosa_roxburghii Rroxscaffold_3G00239450
rosa_rugosa Rorug07G0193200
rosa_samantha Rh7AG334600 Rh7CG352300 Rh7DG331300
rosa_wichuraiana Rw7G028250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 53, 110
AgsI TTSAA 3 cut(s) 28, 289, 560
AluBI AGCT 8 cut(s) 31, 108, 154, 175, 285, 349, 419, 427
AluI AGCT 8 cut(s) 31, 108, 154, 175, 285, 349, 419, 427
Alw26I GTCTC 1 cut(s) 440
AlwNI CAGNNNCTG 1 cut(s) 307
Ama87I CYCGRG 1 cut(s) 443
ApeKI GCWGC 1 cut(s) 175
Asp700I GAANNNNTTC 1 cut(s) 555
AspS9I GGNCC 1 cut(s) 213
AvaI CYCGRG 1 cut(s) 443
AvaII GGWCC 1 cut(s) 213
BanII GRGCYC 1 cut(s) 244
BbsI GAAGAC 1 cut(s) 170
BbvI GCAGC 1 cut(s) 162
BccI CCATC 2 cut(s) 152, 271
BcgI CGANNNNNNTGC 2 cut(s) 434, 468
BciVI GTATCC 2 cut(s) 181, 443
BcoDI GTCTC 1 cut(s) 440
BfmI CTRYAG 1 cut(s) 176
BfuI GTATCC 2 cut(s) 181, 443
BisI GCNGC 1 cut(s) 176
BlsI GCNGC 1 cut(s) 177
Bme18I GGWCC 1 cut(s) 213
BmeT110I CYCGRG 1 cut(s) 443
BmgT120I GGNCC 1 cut(s) 213
BmiI GGNNCC 1 cut(s) 241
BpiI GAAGAC 1 cut(s) 170
BsaJI CCNNGG 3 cut(s) 235, 244, 492
BseDI CCNNGG 3 cut(s) 235, 244, 492
BseGI GGATG 1 cut(s) 163
BseMII CTCAG 1 cut(s) 264
BseRI GAGGAG 2 cut(s) 40, 252
BseXI GCAGC 1 cut(s) 162
BsiHKCI CYCGRG 1 cut(s) 443
BslFI GGGAC 1 cut(s) 59
BsmAI GTCTC 1 cut(s) 440
BsmFI GGGAC 1 cut(s) 59
BsoBI CYCGRG 1 cut(s) 443
Bsp1286I GDGCHC 1 cut(s) 244
Bsp19I CCATGG 2 cut(s) 244, 492
BspCNI CTCAG 1 cut(s) 265
BspLI GGNNCC 1 cut(s) 241
BspMAI CTGCAG 1 cut(s) 180
BssECI CCNNGG 3 cut(s) 235, 244, 492
BssT1I CCWWGG 2 cut(s) 244, 492
Bst4CI ACNGT 3 cut(s) 148, 169, 556
BstC8I GCNNGC 3 cut(s) 347, 480, 499
BstDEI CTNAG 1 cut(s) 273
BstDSI CCRYGG 2 cut(s) 244, 492
BstF5I GGATG 1 cut(s) 163
BstMAI GTCTC 1 cut(s) 440
BstMWI GCNNNNNNNGC 2 cut(s) 114, 461
BstSFI CTRYAG 1 cut(s) 176
BstV1I GCAGC 1 cut(s) 162
BstV2I GAAGAC 1 cut(s) 170
BsuI GTATCC 2 cut(s) 181, 443
BtgI CCRYGG 2 cut(s) 244, 492
BtsCI GGATG 1 cut(s) 163
BtsI GCAGTG 1 cut(s) 308
BtsIMutI CAGTG 1 cut(s) 308
Cac8I GCNNGC 3 cut(s) 347, 480, 499
CaiI CAGNNNCTG 1 cut(s) 307
Cfr13I GGNCC 1 cut(s) 213
CseI GACGC 1 cut(s) 470
CspCI CAANNNNNGTGG 1 cut(s) 580
CviAII CATG 2 cut(s) 245, 493
DdeI CTNAG 1 cut(s) 273
DraI TTTAAA 1 cut(s) 136
DrdI GACNNNNNNGTC 2 cut(s) 53, 110
DseDI GACNNNNNNGTC 2 cut(s) 53, 110
Eco130I CCWWGG 2 cut(s) 244, 492
Eco24I GRGCYC 1 cut(s) 244
Eco47I GGWCC 1 cut(s) 213
Eco88I CYCGRG 1 cut(s) 443
EcoT14I CCWWGG 2 cut(s) 244, 492
EcoT38I GRGCYC 1 cut(s) 244
ErhI CCWWGG 2 cut(s) 244, 492
FaeI CATG 2 cut(s) 248, 496
FaiI YATR 7 cut(s) 15, 99, 218, 246, 264, 327, 494
FaqI GGGAC 1 cut(s) 59
FatI CATG 2 cut(s) 244, 492
Fnu4HI GCNGC 1 cut(s) 176
FokI GGATG 1 cut(s) 170
FriOI GRGCYC 1 cut(s) 244
Fsp4HI GCNGC 1 cut(s) 176
GluI GCNGC 1 cut(s) 176
HgaI GACGC 1 cut(s) 470
Hin1II CATG 2 cut(s) 248, 496
HincII GTYRAC 1 cut(s) 394
HindII GTYRAC 1 cut(s) 394
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 5 cut(s) 47, 81, 92, 207, 571
Hpy166II GTNNAC 2 cut(s) 103, 394
Hpy188I TCNGA 2 cut(s) 59, 80
Hpy188III TCNNGA 4 cut(s) 142, 211, 445, 487
Hpy8I GTNNAC 2 cut(s) 103, 394
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 1 cut(s) 559
HpyCH4III ACNGT 3 cut(s) 148, 169, 556
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 3 cut(s) 178, 329, 345
HpyF10VI GCNNNNNNNGC 2 cut(s) 114, 461
HpyF3I CTNAG 1 cut(s) 273
HpySE526I ACGT 1 cut(s) 111
Hsp92II CATG 2 cut(s) 248, 496
LmnI GCTCC 2 cut(s) 151, 239
LpnPI CCDG 7 cut(s) 127, 140, 180, 196, 390, 464, 483
Lsp1109I GCAGC 1 cut(s) 162
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 2 cut(s) 227, 268
MboII GAAGA 1 cut(s) 175
MfeI CAATTG 1 cut(s) 592
MhlI GDGCHC 1 cut(s) 244
MluCI AATT 3 cut(s) 39, 252, 592
MlyI GAGTC 1 cut(s) 41
MmeI TCCRAC 1 cut(s) 37
MnlI CCTC 3 cut(s) 61, 230, 452
MroXI GAANNNNTTC 1 cut(s) 555
MseI TTAA 1 cut(s) 135
MunI CAATTG 1 cut(s) 592
MwoI GCNNNNNNNGC 2 cut(s) 114, 461
NcoI CCATGG 2 cut(s) 244, 492
NlaIII CATG 2 cut(s) 248, 496
NlaIV GGNNCC 1 cut(s) 241
PaeR7I CTCGAG 1 cut(s) 443
PdmI GAANNNNTTC 1 cut(s) 555
PfeI GAWTC 4 cut(s) 81, 92, 207, 571
PkrI GCNGC 1 cut(s) 177
PleI GAGTC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 41
PspN4I GGNNCC 1 cut(s) 241
PspPI GGNCC 1 cut(s) 213
PstI CTGCAG 1 cut(s) 180
PstNI CAGNNNCTG 1 cut(s) 307
SaqAI TTAA 1 cut(s) 135
SatI GCNGC 1 cut(s) 176
Sau96I GGNCC 1 cut(s) 213
SchI GAGTC 1 cut(s) 41
SduI GDGCHC 1 cut(s) 244
SfcI CTRYAG 1 cut(s) 176
Sfr274I CTCGAG 1 cut(s) 443
SinI GGWCC 1 cut(s) 213
SlaI CTCGAG 1 cut(s) 443
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
Sse9I AATT 3 cut(s) 39, 252, 592
StyI CCWWGG 2 cut(s) 244, 492
TaaI ACNGT 3 cut(s) 148, 169, 556
TaiI ACGT 1 cut(s) 114
TaqI TCGA 1 cut(s) 444
TasI AATT 3 cut(s) 39, 252, 592
TfiI GAWTC 4 cut(s) 81, 92, 207, 571
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TscAI CASTG 1 cut(s) 308
TseI GCWGC 1 cut(s) 175
TspDTI ATGAA 2 cut(s) 348, 578
TspGWI ACGGA 1 cut(s) 43
TspRI CASTG 1 cut(s) 308
VpaK11BI GGWCC 1 cut(s) 213
XhoI CTCGAG 1 cut(s) 443
XmnI GAANNNNTTC 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.