Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0004950

Physical Location & Seq

Genomic Coordinates Reverse (-)

30971615 .. 30983343

Gene Structure 11729 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1244900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2130 bp

ATGACTTATTTTGCAAGCAACGAAGAGGGTCAGTTTTATGATATGAGCAATGATGATGCTCAGAAAGCGGTTTTCGATTTGGGTGCATTTGTTGGGGACTTGACTGTTGAGGAGGATGCAAGCAGCACTGATGATATTTCGTTAGAAGGACTGCAACAAGAATTGGAAGAATGTAAACACGATGATGTAGTTGCGAACATATTGTCTAAAGGCACAAAATTTCGGGAGCATACAAAGGGGGTTGAGAACAATATACGACAAGTTGAATTGGAATCGATCCAGGACTATATTAAAGAAAGTGATAATCTAGTATCTCTTCATGATCAAATTCGTGATTGTGACAGCATTTTGTCACAGATGGAAACTCTTCTGAGTGGATTTCAGTCAGAGATTGGTTCAATAAGTTCAGACATCAAAATTCTCCAGGAGAAGTCTATGGACATGGGTTTGAAGCTTAAGAATCGCAAGGTGGCGGAATCAAAATTGGCTAAGTTTGTTGAAGACATTATAGTCCCACCAAGAATGGTAGACATAATTGTTGAAGGCGAGGTGAATGATGAATATATGAGAACTTTAGAGATTCTTAGTAAGAAGCTGAAGTTTGTAGAAGTAGATCTTATGGTTAAAACTTCAAAAGCCCTAAAAGATGTTCAGCCAGAGCTTGAAAAACTTCGACAGAAAGCAGTTTCCAAGGTGTTTGACTTCGTTGTTCAGAAGCTTTATGCATTGAGAAAACCTAAAACAAATATCCAGATCCTTCAGCAGAATGTTCTTTTGAAGTACAAGTATGTTGTTTCCTTCCTGAAGGAACATGGCAAGGAGGTATATATTGAGGTTCGTGCAGCATACATTGACACAATGAACAAGGTTCTCAGTGCACATTTCCGTGCTTACATTCAAGCACTTGAGAAACTACAGTTGGATATAGCAGCGTCTAGTGATTTAATTGGCGTTGAGACTAGGAACACTAGTCTCTTTTCAAGAGGAAGGGAACCACTGAAGAACCGGTCTGCTGTCTTTGCTCTTGGAGAGAGGAGAAAAGTTTTAAAGGAAATTGATGAACCTGCGTTGATTCCACATATAGCTGAAGCCAGCTCCATCAAGTATCCATACGAGGTCCTCTTCAGGAGCTTGCACAAGCTACTTATGGATACTGCTACTTCCGAGTATCATTTCTGTGATGATTTCTTTGGCGAACAATCCGTATTTTGTGAAATTTTTGCAGGCCCATTTTCTGTAATTGACGAACACTTCAATTCAATACTTCCAAATTGTTACGATGCTATTGGTGTAATGCTAATGATTTGCATAATACATCAGCACCAGCTCATTATGTCTAGGCGGCGTATTCCATGCTTGGATTCATACCTTGACAAGGTCAATATTGCCCTATGGCCTCGCTTTAAGCTTGTGTTTGACCTGCATCTAAACAGCATGCGGAATGCAAATGTGAAGACATTGTGGGAAGATGATGTTCATCCTCACTATGTCATGAGGCGTTATGCTGAATTTACAGCTTCACTTATCCACCTCAATGTGGAATATGGAGATGGGCAGCTTGAATTAAATTTGGAGCGATTGAGAATGGCTATTGACGACTTACTTATCAAGCTTGCTAATTTATTTCCAAAACCAAAGCTACAAACCGTGTTTCTGATAAACAATTATGATATGACGATTGCTGTTTTGAAGGAAGCTGATCCAGAAGGTGGTAAAATTCAAATGCACTTCGAGGAACTTCTGAAAAGCAACACAGCATTATTTGTGGAAGAACTACTACTAGAGCATTTTAGCGATTTAATCAAATTTGTTAAAACCCGAGCTTCGGAGGATCCAAGTGCTAGTTCTGAGAAGCCTATAACTGTTGCGGAGGTTGAGCCCCTCGTGAAGGACTTTGCAAGTAGATGGAAAGCTGCAATCGAACTGATGCACAAAGATGTCATAACTTCTTTCAGCAACTTCCTGTGTGGTATGGAGATCTTGAGGGCGGCGTTGACTCAGCTGTTGCTATATTACACAAGGCTCTCAGATTGCATAAAGAGGATTGTTGGTGGGTCTGCTCTCAACAAGGATCTAGTGTCCATATCGTCAATCATGTACGAAATTAGAAAATACTCGAGGACCTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000139 GO:0000149 GO:0000902 GO:0000904 GO:0000938 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0006810 GO:0006892 GO:0006896 GO:0007034 GO:0008150 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010008 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0016197 GO:0016482 GO:0017016 GO:0017137 GO:0019899 GO:0019905 GO:0019953 GO:0022414 GO:0030154 GO:0031090 GO:0031267 GO:0031410 GO:0031982 GO:0031984 GO:0032456 GO:0032501 GO:0032502 GO:0032588 GO:0032989 GO:0032991 GO:0040007 GO:0042147 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044706 GO:0046907 GO:0048193 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0051020 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051704 GO:0060560 GO:0071840 GO:0097708 GO:0098588 GO:0098791 GO:0098805 GO:0099023

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Vps52

Protein Analysis

710

Amino Acids

81.26

Weight (kDa)

5.5

Isoelectric Point (pI)

43.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sec3_CC	PF09763	78 - 192	1e-07	Exocyst complex component Sec3, coiled-coil
Vps52_CC	PF04129	95 - 268	3.3e-66	Vps52/Sac2 family, coiled-coil
Vps52_C	PF20655	285 - 589	5.3e-126	Vps52, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004950)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G71270 AT1G71300 AT1G71300
fragaria_vesca	FvH4_2g00140 FvH4_2g00140 FvH4_2g00140 FvH4_2g00140 FvH4_2g00140 FvH4_2g00140 FvH4_2g00140 FvH4_2g00140 FvH4_2g00140 FvH4_2g00140
malus_domestica	MD08G1244900.v1.1 MD15G1440000.v1.1
prunus_persica	Prupe.1G566400_v2.0.a1 Prupe.1G566400_v2.0.a1 Prupe.1G566400_v2.0.a1
pyrus_communis	pycom08g21240 pycom15g38790
rosa_chinensis	RchiOBHm_Chr6g0249411
rosa_laevigata	RLG00000015582
rosa_multiflora	Rmu_sc0000278.1_g000001
rosa_roxburghii	Rroxscaffold_7G00217740
rosa_rugosa	Rorug05G0492100
rosa_samantha	Rh6AG002000 Rh6BG008200 Rh6CG001100 Rh6DG001200
rosa_wichuraiana	Rw6G000250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	509
Acc36I	ACCTGC	2 cut(s)	1072, 1428
AccB7I	CCANNNNNTGG	1 cut(s)	1709
AccI	GTMKAC	1 cut(s)	528
AciI	CCGC	6 cut(s)	68, 473, 1342, 1438, 1868, 1988
AclWI	GGATC	6 cut(s)	271, 748, 1694, 1826, 1839, 2079
AcsI	RAATTY	8 cut(s)	218, 327, 417, 1215, 1508, 1567, 1716, 1805
AcuI	CTGAAG	6 cut(s)	617, 743, 824, 1019, 1107, 1108
AfaI	GTAC	2 cut(s)	782, 2099
AfiI	CCNNNNNNNGG	5 cut(s)	1375, 1537, 1709, 1825, 1888
AflII	CTTAAG	1 cut(s)	455
AgeI	ACCGGT	1 cut(s)	1005
AhlI	ACTAGT	1 cut(s)	968
AjnI	CCWGG	2 cut(s)	279, 423
AjuI	GAANNNNNNNTTGG	4 cut(s)	902, 934, 1828, 1860
Alw21I	GWGCWC	1 cut(s)	880
Alw26I	GTCTC	2 cut(s)	950, 977
Alw44I	GTGCAC	1 cut(s)	876
AlwI	GGATC	6 cut(s)	271, 748, 1694, 1826, 1839, 2079
Ama87I	CYCGRG	2 cut(s)	1818, 2116
AoxI	GGCC	2 cut(s)	1225, 1394
ApaLI	GTGCAC	1 cut(s)	876
ApeKI	GCWGC	5 cut(s)	123, 842, 929, 1555, 1913
ApoI	RAATTY	8 cut(s)	218, 327, 417, 1215, 1508, 1567, 1716, 1805
ArsI	GACNNNNNNTTYG	2 cut(s)	188, 220
AsiGI	ACCGGT	1 cut(s)	1005
Asp700I	GAANNNNTTC	2 cut(s)	366, 669
AspS9I	GGNCC	3 cut(s)	1117, 1226, 2121
AsuHPI	GGTGA	1 cut(s)	562
AvaI	CYCGRG	2 cut(s)	1818, 2116
AvaII	GGWCC	2 cut(s)	1117, 2121
BaeGI	GKGCMC	1 cut(s)	880
BamHI	GGATCC	1 cut(s)	1831
BanII	GRGCYC	1 cut(s)	1881
BarI	GAAGNNNNNNTAC	2 cut(s)	1761, 1793
BauI	CACGAG	1 cut(s)	1883
BbsI	GAAGAC	2 cut(s)	507, 1460
Bbv12I	GWGCWC	1 cut(s)	880
BbvI	GCAGC	5 cut(s)	135, 854, 941, 1567, 1900
BccI	CCATC	4 cut(s)	352, 1106, 1544, 1899
BcgI	CGANNNNNNTGC	2 cut(s)	65, 99
BciT130I	CCWGG	2 cut(s)	281, 425
BciVI	GTATCC	2 cut(s)	1116, 1144
BclI	TGATCA	1 cut(s)	322
BcoDI	GTCTC	2 cut(s)	950, 977
BcuI	ACTAGT	1 cut(s)	968
BfaI	CTAG	9 cut(s)	308, 936, 960, 969, 1338, 1781, 1842, 2075, 2128
BfmI	CTRYAG	1 cut(s)	914
BfrI	CTTAAG	1 cut(s)	455
BfuAI	ACCTGC	2 cut(s)	1072, 1428
BfuI	GTATCC	2 cut(s)	1116, 1144
BglII	AGATCT	2 cut(s)	613, 1977
BisI	GCNGC	7 cut(s)	124, 843, 930, 1343, 1556, 1914, 1989
BlsI	GCNGC	7 cut(s)	125, 844, 931, 1344, 1557, 1915, 1990
Bme1390I	CCNGG	2 cut(s)	281, 425
Bme18I	GGWCC	2 cut(s)	1117, 2121
BmeT110I	CYCGRG	2 cut(s)	1818, 2116
BmgT120I	GGNCC	3 cut(s)	1117, 1226, 2121
BmiI	GGNNCC	2 cut(s)	993, 1833
BmrFI	CCNGG	2 cut(s)	281, 425
BmsI	GCATC	5 cut(s)	46, 106, 1270, 1432, 1917
BpiI	GAAGAC	2 cut(s)	507, 1460
BpmI	CTGGAG	1 cut(s)	407
BpuEI	CTTGAG	2 cut(s)	926, 2002
Bsa29I	ATCGAT	1 cut(s)	275
BsaBI	GATNNNNATC	1 cut(s)	1476
BsaJI	CCNNGG	1 cut(s)	690
BsaWI	WCCGGW	1 cut(s)	1005
Bsc4I	CCNNNNNNNGG	5 cut(s)	1375, 1537, 1709, 1825, 1888
Bse118I	RCCGGY	1 cut(s)	1005
Bse3DI	GCAATG	1 cut(s)	55
Bse8I	GATNNNNATC	1 cut(s)	1476
BseBI	CCWGG	2 cut(s)	281, 425
BseCI	ATCGAT	1 cut(s)	275
BseDI	CCNNGG	1 cut(s)	690
BseGI	GGATG	2 cut(s)	121, 1477
BseJI	GATNNNNATC	1 cut(s)	1476
BseLI	CCNNNNNNNGG	5 cut(s)	1375, 1537, 1709, 1825, 1888
BseMI	GCAATG	1 cut(s)	55
BseMII	CTCAG	6 cut(s)	74, 362, 886, 1839, 2012, 2040
BseRI	GAGGAG	2 cut(s)	125, 1048
BseSI	GKGCMC	1 cut(s)	880
BseXI	GCAGC	5 cut(s)	135, 854, 941, 1567, 1900
BsgI	GTGCAG	1 cut(s)	861
BshFI	GGCC	2 cut(s)	1227, 1396
BshTI	ACCGGT	1 cut(s)	1005
BshVI	ATCGAT	1 cut(s)	275
BsiHKAI	GWGCWC	1 cut(s)	880
BsiHKCI	CYCGRG	2 cut(s)	1818, 2116
BsiSI	CCGG	1 cut(s)	1006
BslFI	GGGAC	2 cut(s)	110, 497
BslI	CCNNNNNNNGG	5 cut(s)	1375, 1537, 1709, 1825, 1888
BsmAI	GTCTC	2 cut(s)	950, 977
BsmFI	GGGAC	2 cut(s)	110, 497
BsmI	GAATGC	1 cut(s)	1447
BsnI	GGCC	2 cut(s)	1227, 1396
BsoBI	CYCGRG	2 cut(s)	1818, 2116
Bsp1286I	GDGCHC	2 cut(s)	880, 1881
Bsp143I	GATC	8 cut(s)	276, 322, 613, 753, 1699, 1831, 1977, 2071
BspACI	CCGC	6 cut(s)	68, 473, 1342, 1438, 1868, 1988
BspANI	GGCC	2 cut(s)	1227, 1396
BspCNI	CTCAG	6 cut(s)	73, 363, 885, 1840, 2011, 2039
BspDI	ATCGAT	1 cut(s)	275
BspHI	TCATGA	2 cut(s)	319, 1491
BspLI	GGNNCC	2 cut(s)	993, 1833
BspMI	ACCTGC	2 cut(s)	1072, 1428
BspPI	GGATC	6 cut(s)	271, 748, 1694, 1826, 1839, 2079
BspTI	CTTAAG	1 cut(s)	455
BsrDI	GCAATG	1 cut(s)	55
BsrFI	RCCGGY	1 cut(s)	1005
BssAI	RCCGGY	1 cut(s)	1005
BssECI	CCNNGG	1 cut(s)	690
BssMI	GATC	8 cut(s)	276, 322, 613, 753, 1699, 1831, 1977, 2071
BssSI	CACGAG	1 cut(s)	1883
BssT1I	CCWWGG	1 cut(s)	690
Bst2BI	CACGAG	1 cut(s)	1883
Bst2UI	CCWGG	2 cut(s)	281, 425
Bst4CI	ACNGT	4 cut(s)	106, 918, 1648, 1864
Bst6I	CTCTTC	4 cut(s)	18, 321, 372, 1127
BstAFI	CTTAAG	1 cut(s)	455
BstC8I	GCNNGC	7 cut(s)	16, 121, 1093, 1133, 1225, 1436, 1614
BstDEI	CTNAG	8 cut(s)	60, 371, 489, 584, 872, 1848, 1998, 2026
BstENI	CCTNNNNNAGG	2 cut(s)	1373, 1886
BstF5I	GGATG	2 cut(s)	121, 1477
BstKTI	GATC	8 cut(s)	279, 325, 616, 756, 1702, 1834, 1980, 2074
BstMAI	GTCTC	2 cut(s)	950, 977
BstMBI	GATC	8 cut(s)	276, 322, 613, 753, 1699, 1831, 1977, 2071
BstMWI	GCNNNNNNNGC	2 cut(s)	65, 1019
BstNI	CCWGG	2 cut(s)	281, 425
BstNSI	RCATGY	1 cut(s)	1438
BstSCI	CCNGG	2 cut(s)	279, 423
BstSFI	CTRYAG	1 cut(s)	914
BstSLI	GKGCMC	1 cut(s)	880
BstV1I	GCAGC	5 cut(s)	135, 854, 941, 1567, 1900
BstV2I	GAAGAC	2 cut(s)	507, 1460
BstX2I	RGATCY	5 cut(s)	613, 753, 1831, 1977, 2071
BstYI	RGATCY	5 cut(s)	613, 753, 1831, 1977, 2071
Bsu15I	ATCGAT	1 cut(s)	275
BsuI	GTATCC	2 cut(s)	1116, 1144
BsuRI	GGCC	2 cut(s)	1227, 1396
BsuTUI	ATCGAT	1 cut(s)	275
BtsCI	GGATG	2 cut(s)	121, 1477
BtsIMutI	CAGTG	3 cut(s)	126, 880, 995
BveI	ACCTGC	2 cut(s)	1072, 1428
Cac8I	GCNNGC	7 cut(s)	16, 121, 1093, 1133, 1225, 1436, 1614
CciI	TCATGA	2 cut(s)	319, 1491
Cfr10I	RCCGGY	1 cut(s)	1005
Cfr13I	GGNCC	3 cut(s)	1117, 1226, 2121
ClaI	ATCGAT	1 cut(s)	275
CseI	GACGC	1 cut(s)	921
Csp6I	GTAC	2 cut(s)	781, 2098
CspAI	ACCGGT	1 cut(s)	1005
CviAII	CATG	7 cut(s)	320, 442, 812, 1353, 1435, 1492, 2095
CviQI	GTAC	2 cut(s)	781, 2098
DdeI	CTNAG	8 cut(s)	60, 371, 489, 584, 872, 1848, 1998, 2026
DpnI	GATC	8 cut(s)	278, 324, 615, 755, 1701, 1833, 1979, 2073
DpnII	GATC	8 cut(s)	276, 322, 613, 753, 1699, 1831, 1977, 2071
DraI	TTTAAA	1 cut(s)	1047
DrdI	GACNNNNNNGTC	1 cut(s)	509
DseDI	GACNNNNNNGTC	1 cut(s)	509
Eam1104I	CTCTTC	4 cut(s)	18, 321, 372, 1127
EarI	CTCTTC	4 cut(s)	18, 321, 372, 1127
EciI	GGCGGA	1 cut(s)	488
Eco130I	CCWWGG	1 cut(s)	690
Eco24I	GRGCYC	1 cut(s)	1881
Eco47I	GGWCC	2 cut(s)	1117, 2121
Eco57I	CTGAAG	6 cut(s)	617, 743, 824, 1019, 1107, 1108
Eco88I	CYCGRG	2 cut(s)	1818, 2116
EcoNI	CCTNNNNNAGG	2 cut(s)	1373, 1886
EcoO109I	RGGNCCY	2 cut(s)	1117, 2121
EcoRII	CCWGG	2 cut(s)	279, 423
EcoT14I	CCWWGG	1 cut(s)	690
EcoT22I	ATGCAT	1 cut(s)	727
EcoT38I	GRGCYC	1 cut(s)	1881
ErhI	CCWWGG	1 cut(s)	690
FaeI	CATG	7 cut(s)	323, 445, 815, 1356, 1438, 1495, 2098
FalI	AAGNNNNNCTT	2 cut(s)	600, 632
FaqI	GGGAC	2 cut(s)	110, 497
FatI	CATG	7 cut(s)	319, 441, 811, 1352, 1434, 1491, 2094
FbaI	TGATCA	1 cut(s)	322
FblI	GTMKAC	1 cut(s)	528
Fnu4HI	GCNGC	7 cut(s)	124, 843, 930, 1343, 1556, 1914, 1989
FokI	GGATG	2 cut(s)	128, 1464
FriOI	GRGCYC	1 cut(s)	1881
Fsp4HI	GCNGC	7 cut(s)	124, 843, 930, 1343, 1556, 1914, 1989
FspBI	CTAG	9 cut(s)	308, 936, 960, 969, 1338, 1781, 1842, 2075, 2128
GluI	GCNGC	7 cut(s)	124, 843, 930, 1343, 1556, 1914, 1989
GsuI	CTGGAG	1 cut(s)	407
HaeIII	GGCC	2 cut(s)	1227, 1396
HapII	CCGG	1 cut(s)	1006
HgaI	GACGC	1 cut(s)	921
Hin1II	CATG	7 cut(s)	323, 445, 815, 1356, 1438, 1495, 2098
HincII	GTYRAC	1 cut(s)	1995
HindII	GTYRAC	1 cut(s)	1995
HindIII	AAGCTT	4 cut(s)	452, 716, 1406, 1610
HinfI	GANTC	7 cut(s)	272, 460, 476, 580, 1072, 1361, 1996
HpaII	CCGG	1 cut(s)	1006
HphI	GGTGA	1 cut(s)	562
Hpy166II	GTNNAC	4 cut(s)	176, 529, 878, 1995
Hpy8I	GTNNAC	4 cut(s)	176, 529, 878, 1995
HpyAV	CCTTC	9 cut(s)	140, 536, 767, 799, 808, 981, 1684, 1700, 1882
HpyCH4III	ACNGT	4 cut(s)	106, 918, 1648, 1864
HpyF10VI	GCNNNNNNNGC	2 cut(s)	65, 1019
HpyF3I	CTNAG	8 cut(s)	60, 371, 489, 584, 872, 1848, 1998, 2026
Hsp92II	CATG	7 cut(s)	323, 445, 815, 1356, 1438, 1495, 2098
Ksp22I	TGATCA	1 cut(s)	322
Kzo9I	GATC	8 cut(s)	276, 322, 613, 753, 1699, 1831, 1977, 2071
LmnI	GCTCC	4 cut(s)	226, 1100, 1128, 1573
Lsp1109I	GCAGC	5 cut(s)	135, 854, 941, 1567, 1900
LweI	GCATC	5 cut(s)	46, 106, 1270, 1432, 1917
MaeI	CTAG	9 cut(s)	308, 936, 960, 969, 1338, 1781, 1842, 2075, 2128
MaeIII	GTNAC	3 cut(s)	338, 351, 1274
MalI	GATC	8 cut(s)	278, 324, 615, 755, 1701, 1833, 1979, 2073
MboI	GATC	8 cut(s)	276, 322, 613, 753, 1699, 1831, 1977, 2071
MflI	RGATCY	5 cut(s)	613, 753, 1831, 1977, 2071
MhlI	GDGCHC	2 cut(s)	880, 1881
MlyI	GAGTC	1 cut(s)	1990
MmeI	TCCRAC	1 cut(s)	900
Mph1103I	ATGCAT	1 cut(s)	727
MroXI	GAANNNNTTC	2 cut(s)	366, 669
MseI	TTAA	9 cut(s)	291, 456, 624, 944, 1046, 1404, 1565, 1799, 1812
MslI	CAYNNNNRTG	5 cut(s)	183, 885, 1176, 1533, 1935
MspA1I	CMGCKG	1 cut(s)	2002
MspCI	CTTAAG	1 cut(s)	455
MspI	CCGG	1 cut(s)	1006
MspR9I	CCNGG	2 cut(s)	281, 425
Mva1269I	GAATGC	1 cut(s)	1447
MvaI	CCWGG	2 cut(s)	281, 425
MwoI	GCNNNNNNNGC	2 cut(s)	65, 1019
NdeII	GATC	8 cut(s)	276, 322, 613, 753, 1699, 1831, 1977, 2071
NlaIII	CATG	7 cut(s)	323, 445, 815, 1356, 1438, 1495, 2098
NlaIV	GGNNCC	2 cut(s)	993, 1833
NmuCI	GTSAC	2 cut(s)	338, 351
NsiI	ATGCAT	1 cut(s)	727
NspI	RCATGY	1 cut(s)	1438
PaeI	GCATGC	1 cut(s)	1438
PaeR7I	CTCGAG	1 cut(s)	2116
PagI	TCATGA	2 cut(s)	319, 1491
PctI	GAATGC	1 cut(s)	1447
PdmI	GAANNNNTTC	2 cut(s)	366, 669
PfeI	GAWTC	6 cut(s)	272, 460, 476, 580, 1072, 1361
PflFI	GACNNNGTC	1 cut(s)	1376
PflMI	CCANNNNNTGG	1 cut(s)	1709
PfoI	TCCNGGA	2 cut(s)	279, 423
PinAI	ACCGGT	1 cut(s)	1005
PkrI	GCNGC	7 cut(s)	125, 844, 931, 1344, 1557, 1915, 1990
PleI	GAGTC	1 cut(s)	1990
PpsI	GAGTC	1 cut(s)	1990
PpuMI	RGGWCCY	2 cut(s)	1117, 2121
Psp5II	RGGWCCY	2 cut(s)	1117, 2121
Psp6I	CCWGG	2 cut(s)	279, 423
PspGI	CCWGG	2 cut(s)	279, 423
PspN4I	GGNNCC	2 cut(s)	993, 1833
PspPI	GGNCC	3 cut(s)	1117, 1226, 2121
PspPPI	RGGWCCY	2 cut(s)	1117, 2121
PspXI	VCTCGAGB	1 cut(s)	2116
PsuI	RGATCY	5 cut(s)	613, 753, 1831, 1977, 2071
PsyI	GACNNNGTC	1 cut(s)	1376
PvuII	CAGCTG	1 cut(s)	2002
RsaI	GTAC	2 cut(s)	782, 2099
RsaNI	GTAC	2 cut(s)	781, 2098
RseI	CAYNNNNRTG	5 cut(s)	183, 885, 1176, 1533, 1935
SaqAI	TTAA	9 cut(s)	291, 456, 624, 944, 1046, 1404, 1565, 1799, 1812
SatI	GCNGC	7 cut(s)	124, 843, 930, 1343, 1556, 1914, 1989
Sau3AI	GATC	8 cut(s)	276, 322, 613, 753, 1699, 1831, 1977, 2071
Sau96I	GGNCC	3 cut(s)	1117, 1226, 2121
SchI	GAGTC	1 cut(s)	1990
ScrFI	CCNGG	2 cut(s)	281, 425
SduI	GDGCHC	2 cut(s)	880, 1881
SfaNI	GCATC	5 cut(s)	46, 106, 1270, 1432, 1917
SfcI	CTRYAG	1 cut(s)	914
Sfr274I	CTCGAG	1 cut(s)	2116
SinI	GGWCC	2 cut(s)	1117, 2121
SlaI	CTCGAG	1 cut(s)	2116
SmiMI	CAYNNNNRTG	5 cut(s)	183, 885, 1176, 1533, 1935
SmlI	CTYRAG	4 cut(s)	455, 905, 1981, 2116
SmoI	CTYRAG	4 cut(s)	455, 905, 1981, 2116
SpeI	ACTAGT	1 cut(s)	968
SphI	GCATGC	1 cut(s)	1438
SsiI	CCGC	6 cut(s)	68, 473, 1342, 1438, 1868, 1988
SspI	AATATT	1 cut(s)	1384
SspMI	CTAG	9 cut(s)	308, 936, 960, 969, 1338, 1781, 1842, 2075, 2128
StyD4I	CCNGG	2 cut(s)	279, 423
StyI	CCWWGG	1 cut(s)	690
TaaI	ACNGT	4 cut(s)	106, 918, 1648, 1864
TaqI	TCGA	6 cut(s)	75, 275, 673, 1731, 1920, 2117
TatI	WGTACW	1 cut(s)	780
TauI	GCSGC	2 cut(s)	1345, 1991
TfiI	GAWTC	6 cut(s)	272, 460, 476, 580, 1072, 1361
Tru1I	TTAA	9 cut(s)	291, 456, 624, 944, 1046, 1404, 1565, 1799, 1812
Tru9I	TTAA	9 cut(s)	291, 456, 624, 944, 1046, 1404, 1565, 1799, 1812
TscAI	CASTG	3 cut(s)	133, 880, 1002
TseFI	GTSAC	2 cut(s)	338, 351
TseI	GCWGC	5 cut(s)	123, 842, 929, 1555, 1913
Tsp45I	GTSAC	2 cut(s)	338, 351
TspDTI	ATGAA	6 cut(s)	308, 573, 875, 1074, 1353, 1466
TspGWI	ACGGA	2 cut(s)	875, 1192
TspRI	CASTG	3 cut(s)	133, 880, 1002
Tth111I	GACNNNGTC	1 cut(s)	1376
Van91I	CCANNNNNTGG	1 cut(s)	1709
Vha464I	CTTAAG	1 cut(s)	455
VneI	GTGCAC	1 cut(s)	876
VpaK11BI	GGWCC	2 cut(s)	1117, 2121
XagI	CCTNNNNNAGG	2 cut(s)	1373, 1886
XapI	RAATTY	8 cut(s)	218, 327, 417, 1215, 1508, 1567, 1716, 1805
XceI	RCATGY	1 cut(s)	1438
XhoI	CTCGAG	1 cut(s)	2116
XmiI	GTMKAC	1 cut(s)	528
XmnI	GAANNNNTTC	2 cut(s)	366, 669
XspI	CTAG	9 cut(s)	308, 936, 960, 969, 1338, 1781, 1842, 2075, 2128
Zsp2I	ATGCAT	1 cut(s)	727

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.