Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0004950

Physical Location & Seq

Genomic Coordinates Reverse (-)

53986724 .. 53998783

Gene Structure 12060 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1440000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2133 bp

ATGGCTTACGTTTCGAGCAACAAAGAGGGAGACTCTTATGATGTGAGCAATGGTGATGCTCCGAAAACGGTTTTCGATTTGGGTGCATTTGTTGGGGACTTGACTGTTGAGGAGGACGCAAGCAGTGATGATATATCGTTAGAAGGACTGCAGCAAGAATTGGAAGAATGTAAACACGATGATGTAGTTGCGAACATATTGTCTAAAGGCACGAAACTTCGGGAGCATACAAAGGGGGTTGAGAACAATATACGACAAGTTGAATTGGACTCCATCCAGGTTCTTGACTATATTAAAGAAAGTGATAATCTGGTATCTCTTCATGATCAAATTCGTGATTGTGACAGCATTTTGTCACAGATGGAAACTCTTCTGAGTGGATTTCAGTCAGAGATTGGTTCAATAAGTTCAGACATCAAAATTCTCCAGGAGAAGTCTATGGACATGGGTTTGAAGCTGAAGAATCGCAAGGTGGCAGAATCAAAATTGGCAAAGTTTGTTGAAGATATTATAGTCCCTCCAAGAATGGTAGACATAATTGTTGAAGGCGAGGTGAATGATGAATATATGAGAACTTTAGAGATTCTAAGTAAGAAGCTCAAGTTTGTAGAAGTAGATCTTATGGTTAAAACTTCAAAAGCCCTAAAAGATGTTCAGCCCGAGCTTGAAAAACTCCGACAGAAAGCAGTTTCCAAGGTGTTTGACTTCATTGTTCAGAAGCTTTATGCATTGAGAAAACCTAAAACAAATATCCAGATCCTTCAGCAGAATGTTCTTTTGAAGTACAAGTACGTTGTTTCCTTCCTGAAGGAACATGGCAAGGAGGTATATATTGAGGTTCGCGGAGCATACATTGACACAATGAACAAGGTTCTCAGTGCACATTTCCGCGCTTACATTCAAGCACTTGAGAAACTACAGTTGGATATAGCAACATCTAATGATTTAATTGGTGTTGAGACTAGAAACACTAGTCTCTTTTTAAGAGGAAGGGAACCACTGAAGAACCGGTCTGCTGTCTTTGCCCTTGGGGAGAGGAGAAAAATTTTAAAGGAAATTGAGGAACCTGCATTGATTCCACATATAGCTGAAGCCAGCAACATCAAGTATCCATACGAGGTCCTCTTCAGGAGCTTGCACAAGCTACTTATGGATACTGCTACTTCCGAGTATCATTTCTGCGATGATTTCTTTGGTGAAGAATCCATATTTTATGAAATTTTTGCAGGCCCATTTTCTGTAATTGACGAGCACTTCAATTCAATACTTCCAAATTGTTACGATGCTATTGGGGTAATGCTTATGATTCGCATAATACACCAGCACCAGCTAATTATGTCTAGGCGGCGTATTCCATGCTTGGACTCATACCTTGATAAGGTCAATATTGCCCTATGGCCTCGCTTTAAGATGGTATTTGACCTGCATCTAAACAGTCTGCGGAATGCAAATGTGAAGACATTGTGGGAAGATGATGTTCATCCTCACTATGTCATGAGGCGTTATGCTGAATTTACAGCTTCACTTATCCACCTCAATGTGGAATATGGAGATGGGCAGCTTGAATTAAATTTGGAGCGGCTGAGAATGGCTATTGACGACTTGCTTATCAAGCTTGCTAAATCATTTCCAAAACCAAAACTACAAACTGTGTTTCTGATAAACAATTATGATATGACGATTGCTGTGTTGAAGGAAGCTGATCCAGAAGGTGGTAAAATTCAAATGCACTTTGAGGAACTTCTGAAAAGCAACACGGCATTATTTGTGGAAGAACTACTACTAGAGCATTTTGGCGATTTAATCAAGTTTGTTAAAACCCGAGCTTCGGAGGATCCAAGTGCTAGTTCTGAGAGGCCTATAACTGTTGCGGAGGTTGAGCCCCTTGTGAAGGACTTTGCAAGTAGATGGAAAGCTGCAATCGAACTGATGCACAAAGACGTCATAACTTCTTTCAGCAACTTCTTGTGTGGTATGGAGATCTTGAGGGCGGCATTGACTCAGCTGTTGCTCTATTACACTAGGCTCTCGGATTGCATAAAGAGGGTTGTTGGTGGGTCTGCTCTCAACAAGGATCTAGTGTCCATACCGTCAATCATGTACGAGATTAGAAAATACTCGAGGACTTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000139 GO:0000149 GO:0000902 GO:0000904 GO:0000938 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0006810 GO:0006892 GO:0006896 GO:0007034 GO:0008150 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010008 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0016197 GO:0016482 GO:0017016 GO:0017137 GO:0019899 GO:0019905 GO:0019953 GO:0022414 GO:0030154 GO:0031090 GO:0031267 GO:0031410 GO:0031982 GO:0031984 GO:0032456 GO:0032501 GO:0032502 GO:0032588 GO:0032989 GO:0032991 GO:0040007 GO:0042147 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044706 GO:0046907 GO:0048193 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0051020 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051704 GO:0060560 GO:0071840 GO:0097708 GO:0098588 GO:0098791 GO:0098805 GO:0099023

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Vps52

Protein Analysis

711

Amino Acids

81.35

Weight (kDa)

5.64

Isoelectric Point (pI)

45.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps52_CC	PF04129	96 - 269	1.2e-66	Vps52/Sac2 family, coiled-coil
Vps52_C	PF20655	286 - 590	3.1e-128	Vps52, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004950)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G71270 AT1G71300 AT1G71300
fragaria_vesca	FvH4_2g00140 FvH4_2g00140 FvH4_2g00140 FvH4_2g00140 FvH4_2g00140 FvH4_2g00140 FvH4_2g00140 FvH4_2g00140 FvH4_2g00140 FvH4_2g00140
malus_domestica	MD08G1244900.v1.1 MD15G1440000.v1.1
prunus_persica	Prupe.1G566400_v2.0.a1 Prupe.1G566400_v2.0.a1 Prupe.1G566400_v2.0.a1
pyrus_communis	pycom08g21240 pycom15g38790
rosa_chinensis	RchiOBHm_Chr6g0249411
rosa_laevigata	RLG00000015582
rosa_multiflora	Rmu_sc0000278.1_g000001
rosa_roxburghii	Rroxscaffold_7G00217740
rosa_rugosa	Rorug05G0492100
rosa_samantha	Rh6AG002000 Rh6BG008200 Rh6CG001100 Rh6DG001200
rosa_wichuraiana	Rw6G000250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1944
Acc36I	ACCTGC	2 cut(s)	1075, 1431
AccB7I	CCANNNNNTGG	1 cut(s)	1712
AccBSI	CCGCTC	1 cut(s)	1579
AccI	GTMKAC	1 cut(s)	531
AccII	CGCG	2 cut(s)	843, 891
AciI	CCGC	7 cut(s)	843, 889, 1345, 1441, 1579, 1871, 1991
AclWI	GGATC	5 cut(s)	751, 1697, 1829, 1842, 2082
AcsI	RAATTY	7 cut(s)	330, 420, 1044, 1218, 1511, 1570, 1719
AcuI	CTGAAG	6 cut(s)	479, 746, 827, 1022, 1110, 1111
AcyI	GRCGYC	1 cut(s)	1941
AfaI	GTAC	3 cut(s)	785, 791, 2102
AfiI	CCNNNNNNNGG	5 cut(s)	1378, 1540, 1712, 1828, 1891
AgeI	ACCGGT	1 cut(s)	1008
AhlI	ACTAGT	1 cut(s)	971
AjnI	CCWGG	2 cut(s)	276, 426
AjuI	GAANNNNNNNTTGG	4 cut(s)	905, 937, 1831, 1863
Alw21I	GWGCWC	2 cut(s)	883, 1254
Alw26I	GTCTC	3 cut(s)	24, 953, 980
Alw44I	GTGCAC	1 cut(s)	879
AlwI	GGATC	5 cut(s)	751, 1697, 1829, 1842, 2082
Ama87I	CYCGRG	3 cut(s)	659, 1821, 2119
AoxI	GGCC	3 cut(s)	1228, 1397, 1856
ApaLI	GTGCAC	1 cut(s)	879
ApeKI	GCWGC	3 cut(s)	151, 1558, 1916
ApoI	RAATTY	7 cut(s)	330, 420, 1044, 1218, 1511, 1570, 1719
ArsI	GACNNNNNNTTYG	2 cut(s)	185, 217
AsiGI	ACCGGT	1 cut(s)	1008
Asp700I	GAANNNNTTC	1 cut(s)	369
AspLEI	GCGC	1 cut(s)	893
AspS9I	GGNCC	2 cut(s)	1120, 1229
AsuHPI	GGTGA	3 cut(s)	65, 565, 1208
AvaI	CYCGRG	3 cut(s)	659, 1821, 2119
AvaII	GGWCC	1 cut(s)	1120
BaeGI	GKGCMC	1 cut(s)	883
BamHI	GGATCC	1 cut(s)	1834
BanII	GRGCYC	1 cut(s)	1884
BarI	GAAGNNNNNNTAC	4 cut(s)	773, 805, 1764, 1796
BbsI	GAAGAC	1 cut(s)	1463
Bbv12I	GWGCWC	2 cut(s)	883, 1254
BbvI	GCAGC	3 cut(s)	163, 1570, 1903
BccI	CCATC	5 cut(s)	281, 355, 1405, 1547, 1902
BceAI	ACGGC	1 cut(s)	1773
BcgI	CGANNNNNNTGC	2 cut(s)	65, 99
BciT130I	CCWGG	2 cut(s)	278, 428
BciVI	GTATCC	2 cut(s)	1119, 1147
BclI	TGATCA	1 cut(s)	325
BcoDI	GTCTC	3 cut(s)	24, 953, 980
BcuI	ACTAGT	1 cut(s)	971
BfaI	CTAG	8 cut(s)	963, 972, 1341, 1784, 1845, 2022, 2078, 2131
BfmI	CTRYAG	2 cut(s)	149, 917
BfuAI	ACCTGC	2 cut(s)	1075, 1431
BfuI	GTATCC	2 cut(s)	1119, 1147
BglII	AGATCT	2 cut(s)	616, 1980
BisI	GCNGC	6 cut(s)	152, 1346, 1559, 1580, 1917, 1992
BlsI	GCNGC	6 cut(s)	153, 1347, 1560, 1581, 1918, 1993
Bme1390I	CCNGG	2 cut(s)	278, 428
Bme18I	GGWCC	1 cut(s)	1120
BmeT110I	CYCGRG	3 cut(s)	659, 1821, 2119
BmgT120I	GGNCC	2 cut(s)	1120, 1229
BmiI	GGNNCC	3 cut(s)	996, 1065, 1836
BmrFI	CCNGG	2 cut(s)	278, 428
BmsI	GCATC	4 cut(s)	46, 1273, 1435, 1920
BpiI	GAAGAC	1 cut(s)	1463
BplI	GAGNNNNNCTC	2 cut(s)	17, 49
BpmI	CTGGAG	1 cut(s)	410
BpuEI	CTTGAG	3 cut(s)	584, 929, 2005
BsaBI	GATNNNNATC	1 cut(s)	1479
BsaHI	GRCGYC	1 cut(s)	1941
BsaJI	CCNNGG	2 cut(s)	693, 1027
BsaWI	WCCGGW	1 cut(s)	1008
Bsc4I	CCNNNNNNNGG	5 cut(s)	1378, 1540, 1712, 1828, 1891
Bse118I	RCCGGY	1 cut(s)	1008
Bse3DI	GCAATG	1 cut(s)	55
Bse8I	GATNNNNATC	1 cut(s)	1479
BseBI	CCWGG	2 cut(s)	278, 428
BseDI	CCNNGG	2 cut(s)	693, 1027
BseGI	GGATG	2 cut(s)	273, 1480
BseJI	GATNNNNATC	1 cut(s)	1479
BseLI	CCNNNNNNNGG	5 cut(s)	1378, 1540, 1712, 1828, 1891
BseMI	GCAATG	1 cut(s)	55
BseMII	CTCAG	5 cut(s)	365, 889, 1574, 1842, 2015
BseRI	GAGGAG	2 cut(s)	125, 1051
BseSI	GKGCMC	1 cut(s)	883
BseXI	GCAGC	3 cut(s)	163, 1570, 1903
Bsh1236I	CGCG	2 cut(s)	843, 891
BshFI	GGCC	3 cut(s)	1230, 1399, 1858
BshTI	ACCGGT	1 cut(s)	1008
BsiHKAI	GWGCWC	2 cut(s)	883, 1254
BsiHKCI	CYCGRG	3 cut(s)	659, 1821, 2119
BsiSI	CCGG	1 cut(s)	1009
BslFI	GGGAC	2 cut(s)	110, 500
BslI	CCNNNNNNNGG	5 cut(s)	1378, 1540, 1712, 1828, 1891
BsmAI	GTCTC	3 cut(s)	24, 953, 980
BsmFI	GGGAC	2 cut(s)	110, 500
BsmI	GAATGC	1 cut(s)	1450
BsnI	GGCC	3 cut(s)	1230, 1399, 1858
BsoBI	CYCGRG	3 cut(s)	659, 1821, 2119
Bsp1286I	GDGCHC	3 cut(s)	883, 1254, 1884
Bsp143I	GATC	7 cut(s)	325, 616, 756, 1702, 1834, 1980, 2074
BspACI	CCGC	7 cut(s)	843, 889, 1345, 1441, 1579, 1871, 1991
BspANI	GGCC	3 cut(s)	1230, 1399, 1858
BspCNI	CTCAG	5 cut(s)	366, 888, 1575, 1843, 2014
BspFNI	CGCG	2 cut(s)	843, 891
BspHI	TCATGA	2 cut(s)	322, 1494
BspLI	GGNNCC	3 cut(s)	996, 1065, 1836
BspMAI	CTGCAG	1 cut(s)	153
BspMI	ACCTGC	2 cut(s)	1075, 1431
BspPI	GGATC	5 cut(s)	751, 1697, 1829, 1842, 2082
BsrBI	CCGCTC	1 cut(s)	1579
BsrDI	GCAATG	1 cut(s)	55
BsrFI	RCCGGY	1 cut(s)	1008
BssAI	RCCGGY	1 cut(s)	1008
BssECI	CCNNGG	2 cut(s)	693, 1027
BssMI	GATC	7 cut(s)	325, 616, 756, 1702, 1834, 1980, 2074
BssNI	GRCGYC	1 cut(s)	1941
BssT1I	CCWWGG	2 cut(s)	693, 1027
Bst2UI	CCWGG	2 cut(s)	278, 428
Bst4CI	ACNGT	7 cut(s)	70, 106, 921, 1436, 1651, 1867, 2091
Bst6I	CTCTTC	3 cut(s)	324, 375, 1130
BstACI	GRCGYC	1 cut(s)	1941
BstC8I	GCNNGC	5 cut(s)	121, 1096, 1136, 1228, 1617
BstDEI	CTNAG	6 cut(s)	374, 587, 875, 1583, 1851, 2001
BstENI	CCTNNNNNAGG	2 cut(s)	1376, 1889
BstF5I	GGATG	2 cut(s)	273, 1480
BstFNI	CGCG	2 cut(s)	843, 891
BstHHI	GCGC	1 cut(s)	893
BstKTI	GATC	7 cut(s)	328, 619, 759, 1705, 1837, 1983, 2077
BstMAI	GTCTC	3 cut(s)	24, 953, 980
BstMBI	GATC	7 cut(s)	325, 616, 756, 1702, 1834, 1980, 2074
BstMWI	GCNNNNNNNGC	2 cut(s)	1022, 1612
BstNI	CCWGG	2 cut(s)	278, 428
BstSCI	CCNGG	2 cut(s)	276, 426
BstSFI	CTRYAG	2 cut(s)	149, 917
BstSLI	GKGCMC	1 cut(s)	883
BstUI	CGCG	2 cut(s)	843, 891
BstV1I	GCAGC	3 cut(s)	163, 1570, 1903
BstV2I	GAAGAC	1 cut(s)	1463
BstX2I	RGATCY	5 cut(s)	616, 756, 1834, 1980, 2074
BstYI	RGATCY	5 cut(s)	616, 756, 1834, 1980, 2074
BsuI	GTATCC	2 cut(s)	1119, 1147
BsuRI	GGCC	3 cut(s)	1230, 1399, 1858
BtgZI	GCGATG	1 cut(s)	1197
BtsCI	GGATG	2 cut(s)	273, 1480
BtsI	GCAGTG	1 cut(s)	130
BtsIMutI	CAGTG	3 cut(s)	130, 883, 998
BveI	ACCTGC	2 cut(s)	1075, 1431
Cac8I	GCNNGC	5 cut(s)	121, 1096, 1136, 1228, 1617
CciI	TCATGA	2 cut(s)	322, 1494
CfoI	GCGC	1 cut(s)	893
Cfr10I	RCCGGY	1 cut(s)	1008
Cfr13I	GGNCC	2 cut(s)	1120, 1229
CseI	GACGC	1 cut(s)	125
Csp6I	GTAC	3 cut(s)	784, 790, 2101
CspAI	ACCGGT	1 cut(s)	1008
CviAII	CATG	6 cut(s)	323, 445, 815, 1356, 1495, 2098
CviQI	GTAC	3 cut(s)	784, 790, 2101
DdeI	CTNAG	6 cut(s)	374, 587, 875, 1583, 1851, 2001
DpnI	GATC	7 cut(s)	327, 618, 758, 1704, 1836, 1982, 2076
DpnII	GATC	7 cut(s)	325, 616, 756, 1702, 1834, 1980, 2074
DraI	TTTAAA	1 cut(s)	1050
Eam1104I	CTCTTC	3 cut(s)	324, 375, 1130
EarI	CTCTTC	3 cut(s)	324, 375, 1130
Eco130I	CCWWGG	2 cut(s)	693, 1027
Eco147I	AGGCCT	1 cut(s)	1858
Eco24I	GRGCYC	1 cut(s)	1884
Eco47I	GGWCC	1 cut(s)	1120
Eco57I	CTGAAG	6 cut(s)	479, 746, 827, 1022, 1110, 1111
Eco88I	CYCGRG	3 cut(s)	659, 1821, 2119
EcoNI	CCTNNNNNAGG	2 cut(s)	1376, 1889
EcoO109I	RGGNCCY	1 cut(s)	1120
EcoRII	CCWGG	2 cut(s)	276, 426
EcoT14I	CCWWGG	2 cut(s)	693, 1027
EcoT22I	ATGCAT	1 cut(s)	730
EcoT38I	GRGCYC	1 cut(s)	1884
ErhI	CCWWGG	2 cut(s)	693, 1027
FaeI	CATG	6 cut(s)	326, 448, 818, 1359, 1498, 2101
FalI	AAGNNNNNCTT	2 cut(s)	603, 635
FaqI	GGGAC	2 cut(s)	110, 500
FatI	CATG	6 cut(s)	322, 444, 814, 1355, 1494, 2097
FbaI	TGATCA	1 cut(s)	325
FblI	GTMKAC	1 cut(s)	531
Fnu4HI	GCNGC	6 cut(s)	152, 1346, 1559, 1580, 1917, 1992
FokI	GGATG	2 cut(s)	260, 1467
FriOI	GRGCYC	1 cut(s)	1884
Fsp4HI	GCNGC	6 cut(s)	152, 1346, 1559, 1580, 1917, 1992
FspBI	CTAG	8 cut(s)	963, 972, 1341, 1784, 1845, 2022, 2078, 2131
GlaI	GCGC	1 cut(s)	892
GluI	GCNGC	6 cut(s)	152, 1346, 1559, 1580, 1917, 1992
GsuI	CTGGAG	1 cut(s)	410
HaeIII	GGCC	3 cut(s)	1230, 1399, 1858
HapII	CCGG	1 cut(s)	1009
HgaI	GACGC	1 cut(s)	125
HhaI	GCGC	1 cut(s)	893
Hin1I	GRCGYC	1 cut(s)	1941
Hin1II	CATG	6 cut(s)	326, 448, 818, 1359, 1498, 2101
Hin6I	GCGC	1 cut(s)	891
HinP1I	GCGC	1 cut(s)	891
HindIII	AAGCTT	2 cut(s)	719, 1613
HpaII	CCGG	1 cut(s)	1009
HphI	GGTGA	3 cut(s)	65, 565, 1208
Hpy166II	GTNNAC	3 cut(s)	173, 532, 881
Hpy8I	GTNNAC	3 cut(s)	173, 532, 881
HpyAV	CCTTC	9 cut(s)	137, 539, 770, 802, 811, 984, 1687, 1703, 1885
HpyCH4III	ACNGT	7 cut(s)	70, 106, 921, 1436, 1651, 1867, 2091
HpyCH4IV	ACGT	3 cut(s)	9, 792, 1941
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1022, 1612
HpyF3I	CTNAG	6 cut(s)	374, 587, 875, 1583, 1851, 2001
HpySE526I	ACGT	3 cut(s)	9, 792, 1941
Hsp92I	GRCGYC	1 cut(s)	1941
Hsp92II	CATG	6 cut(s)	326, 448, 818, 1359, 1498, 2101
HspAI	GCGC	1 cut(s)	891
Ksp22I	TGATCA	1 cut(s)	325
Kzo9I	GATC	7 cut(s)	325, 616, 756, 1702, 1834, 1980, 2074
LmnI	GCTCC	5 cut(s)	64, 223, 845, 1131, 1576
Lsp1109I	GCAGC	3 cut(s)	163, 1570, 1903
LweI	GCATC	4 cut(s)	46, 1273, 1435, 1920
MaeI	CTAG	8 cut(s)	963, 972, 1341, 1784, 1845, 2022, 2078, 2131
MaeII	ACGT	3 cut(s)	9, 792, 1941
MaeIII	GTNAC	3 cut(s)	341, 354, 1277
MalI	GATC	7 cut(s)	327, 618, 758, 1704, 1836, 1982, 2076
MbiI	CCGCTC	1 cut(s)	1579
MboI	GATC	7 cut(s)	325, 616, 756, 1702, 1834, 1980, 2074
MflI	RGATCY	5 cut(s)	616, 756, 1834, 1980, 2074
MhlI	GDGCHC	3 cut(s)	883, 1254, 1884
MlyI	GAGTC	4 cut(s)	26, 263, 1358, 1993
MmeI	TCCRAC	2 cut(s)	700, 903
Mph1103I	ATGCAT	1 cut(s)	730
MroXI	GAANNNNTTC	1 cut(s)	369
MseI	TTAA	9 cut(s)	294, 627, 947, 983, 1049, 1407, 1568, 1802, 1815
MslI	CAYNNNNRTG	2 cut(s)	180, 1536
MspA1I	CMGCKG	1 cut(s)	2005
MspI	CCGG	1 cut(s)	1009
MspR9I	CCNGG	2 cut(s)	278, 428
Mva1269I	GAATGC	1 cut(s)	1450
MvaI	CCWGG	2 cut(s)	278, 428
MvnI	CGCG	2 cut(s)	843, 891
MwoI	GCNNNNNNNGC	2 cut(s)	1022, 1612
NdeII	GATC	7 cut(s)	325, 616, 756, 1702, 1834, 1980, 2074
NlaIII	CATG	6 cut(s)	326, 448, 818, 1359, 1498, 2101
NlaIV	GGNNCC	3 cut(s)	996, 1065, 1836
NmuCI	GTSAC	2 cut(s)	341, 354
NsiI	ATGCAT	1 cut(s)	730
PaeR7I	CTCGAG	1 cut(s)	2119
PagI	TCATGA	2 cut(s)	322, 1494
PceI	AGGCCT	1 cut(s)	1858
PctI	GAATGC	1 cut(s)	1450
PdmI	GAANNNNTTC	1 cut(s)	369
PfeI	GAWTC	6 cut(s)	463, 479, 583, 1075, 1202, 1306
PflMI	CCANNNNNTGG	1 cut(s)	1712
PfoI	TCCNGGA	1 cut(s)	426
PinAI	ACCGGT	1 cut(s)	1008
PkrI	GCNGC	6 cut(s)	153, 1347, 1560, 1581, 1918, 1993
PleI	GAGTC	4 cut(s)	26, 263, 1358, 1993
PpsI	GAGTC	4 cut(s)	26, 263, 1358, 1993
PpuMI	RGGWCCY	1 cut(s)	1120
Psp5II	RGGWCCY	1 cut(s)	1120
Psp6I	CCWGG	2 cut(s)	276, 426
PspGI	CCWGG	2 cut(s)	276, 426
PspN4I	GGNNCC	3 cut(s)	996, 1065, 1836
PspPI	GGNCC	2 cut(s)	1120, 1229
PspPPI	RGGWCCY	1 cut(s)	1120
PspXI	VCTCGAGB	1 cut(s)	2119
PstI	CTGCAG	1 cut(s)	153
PsuI	RGATCY	5 cut(s)	616, 756, 1834, 1980, 2074
PvuII	CAGCTG	1 cut(s)	2005
RsaI	GTAC	3 cut(s)	785, 791, 2102
RsaNI	GTAC	3 cut(s)	784, 790, 2101
RseI	CAYNNNNRTG	2 cut(s)	180, 1536
SaqAI	TTAA	9 cut(s)	294, 627, 947, 983, 1049, 1407, 1568, 1802, 1815
SatI	GCNGC	6 cut(s)	152, 1346, 1559, 1580, 1917, 1992
Sau3AI	GATC	7 cut(s)	325, 616, 756, 1702, 1834, 1980, 2074
Sau96I	GGNCC	2 cut(s)	1120, 1229
SchI	GAGTC	4 cut(s)	26, 263, 1358, 1993
ScrFI	CCNGG	2 cut(s)	278, 428
SduI	GDGCHC	3 cut(s)	883, 1254, 1884
SfaNI	GCATC	4 cut(s)	46, 1273, 1435, 1920
SfcI	CTRYAG	2 cut(s)	149, 917
Sfr274I	CTCGAG	1 cut(s)	2119
SinI	GGWCC	1 cut(s)	1120
SlaI	CTCGAG	1 cut(s)	2119
SmiMI	CAYNNNNRTG	2 cut(s)	180, 1536
SmlI	CTYRAG	4 cut(s)	599, 908, 1984, 2119
SmoI	CTYRAG	4 cut(s)	599, 908, 1984, 2119
SpeI	ACTAGT	1 cut(s)	971
SseBI	AGGCCT	1 cut(s)	1858
SsiI	CCGC	7 cut(s)	843, 889, 1345, 1441, 1579, 1871, 1991
SspI	AATATT	1 cut(s)	1387
SspMI	CTAG	8 cut(s)	963, 972, 1341, 1784, 1845, 2022, 2078, 2131
StuI	AGGCCT	1 cut(s)	1858
StyD4I	CCNGG	2 cut(s)	276, 426
StyI	CCWWGG	2 cut(s)	693, 1027
TaaI	ACNGT	7 cut(s)	70, 106, 921, 1436, 1651, 1867, 2091
TaiI	ACGT	3 cut(s)	12, 795, 1944
TaqI	TCGA	4 cut(s)	14, 75, 1923, 2120
TatI	WGTACW	1 cut(s)	783
TauI	GCSGC	3 cut(s)	1348, 1582, 1994
TfiI	GAWTC	6 cut(s)	463, 479, 583, 1075, 1202, 1306
Tru1I	TTAA	9 cut(s)	294, 627, 947, 983, 1049, 1407, 1568, 1802, 1815
Tru9I	TTAA	9 cut(s)	294, 627, 947, 983, 1049, 1407, 1568, 1802, 1815
TscAI	CASTG	3 cut(s)	130, 883, 1005
TseFI	GTSAC	2 cut(s)	341, 354
TseI	GCWGC	3 cut(s)	151, 1558, 1916
Tsp45I	GTSAC	2 cut(s)	341, 354
TspDTI	ATGAA	6 cut(s)	311, 576, 697, 878, 1230, 1469
TspRI	CASTG	3 cut(s)	130, 883, 1005
Van91I	CCANNNNNTGG	1 cut(s)	1712
VneI	GTGCAC	1 cut(s)	879
VpaK11BI	GGWCC	1 cut(s)	1120
XagI	CCTNNNNNAGG	2 cut(s)	1376, 1889
XapI	RAATTY	7 cut(s)	330, 420, 1044, 1218, 1511, 1570, 1719
XhoI	CTCGAG	1 cut(s)	2119
XmiI	GTMKAC	1 cut(s)	531
XmnI	GAANNNNTTC	1 cut(s)	369
XspI	CTAG	8 cut(s)	963, 972, 1341, 1784, 1845, 2022, 2078, 2131
ZraI	GACGTC	1 cut(s)	1942
Zsp2I	ATGCAT	1 cut(s)	730

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.