Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0008768

Physical Location & Seq

Genomic Coordinates Forward (+)

31508061 .. 31513676

Gene Structure 5616 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1250200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1020 bp

ATGTTCTGGAGGGACCGTGACCGTGAGAGGGACAACAAAGACCAAAACGGTGGCGGCGGTCCGCCTTGTGGCCAGGTTCGGGTGCTGGTTGTTGGCGACTCTGGTGTTGGAAAAACTGCTCTTGTTCATCTGATTGTCAAAGGTTCTTCCATCACGCGTCCTCCTCAGACAGTAGGGTGTACAGTAGGTGTAAAGCATACTACTTATGGAAACTCTGGTAGCTCGTCAAGCAGTATTAAAGGTGACGCTGAGAGAGATTTCTTTATTGAACTCTGGGATGTGTCTGGACATGATAGATACAAAGAATGCCGGTCTCTTTTTTATTCCCAAATTAATGGTGTAATCTTTGTTCATGATCTCTCCCAGAGGAGGACAAAAACTGGCTTACAGAAGTGGGCAGCTGAGATTGCTGCAACTGGGACATTTTCGGCTCCTCTAGGATCTGGAGGTCCTGGTGGCCTTCCTGTCCCGTATATTGTTATAGGTAACAAAGCTGATATTGCTGAAGAGGGTACAAGGGGAAGCAGTGGCAATCTTGTTGATGTAGCTCGACAGTGGGTTGAGAAGCAAGGTTTGCTTCCATCCAGTGAGGAACTTCCACTGACTGAGGGTTTCCCTGGTGCTGGAGGCCTTATTGCGGCAGCCAAAGAAGCAAGATATGACAAGGAAGCTGTGATGAAATTTTTCCGTACGTTGATCAAAAGAAGATATTTTTCCGATGATTTACCTGCACAGAATCCATGGTCTAGTTCTCCAGTACAAAGACCTGCACAAACTGTAGATGAAAATTGGAGTGACGAGGATCATTCCTACAGGAATGCCAGCTTGCGTGCTGATCCTTACAAGTACAACATGCTCCCTCCTCTTCCAGCACAACGCAATCTGACACCACCTCCCACACTTTATCCTCAGCAACCAGTTTCGGTTTCTGAAAACTACAGACTTCCTAGGTTTTCCCATAATAGTTACTCAGAAATCAGCAGTGCAGCTAGATCAAAGCGCTCCGATATTAATGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003924 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0007275 GO:0007623 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009791 GO:0010468 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0019222 GO:0032501 GO:0032502 GO:0032922 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048511 GO:0048856 GO:0050789 GO:0050896 GO:0060255 GO:0065007

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ras Roc

Protein Analysis

340

Amino Acids

37.2

Weight (kDa)

8.83

Isoelectric Point (pI)

51.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Roc	PF08477	27 - 140	1.9e-15	Ras of Complex, Roc, domain of DAPkinase
Ras	PF00071	27 - 170	2.1e-15	Ras family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008768)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64813 AT5G64813 AT5G64813
fragaria_vesca	FvH4_1g22810
malus_domestica	MD08G1250200.v1.1 MD15G1445400.v1.1
prunus_persica	Prupe.1G587300_v2.0.a1 Prupe.1G587300_v2.0.a1 Prupe.1G587300_v2.0.a1 Prupe.1G587300_v2.0.a1 Prupe.1G587300_v2.0.a1
pyrus_communis	pycom08g21700 pycom15g39230
rosa_chinensis	RchiOBHm_Chr2g0117121
rosa_laevigata	RLG00000018332
rosa_multiflora	Rmu_sc0005833.1_g000006
rosa_roxburghii	Rroxscaffold_2G00126690
rosa_rugosa	Rorug02G0210700
rosa_samantha	Rh2AG266400 Rh2BG278800 Rh2DG273600 Rh2DG292700
rosa_wichuraiana	Rw2G021030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	736, 775
AccII	CGCG	1 cut(s)	157
AciI	CCGC	4 cut(s)	54, 57, 62, 638
AclWI	GGATC	3 cut(s)	448, 810, 830
AcoI	YGGCCR	1 cut(s)	70
AcsI	RAATTY	1 cut(s)	680
AcuI	CTGAAG	1 cut(s)	525
AfaI	GTAC	5 cut(s)	181, 514, 691, 759, 848
AfeI	AGCGCT	1 cut(s)	1001
AfiI	CCNNNNNNNGG	6 cut(s)	28, 68, 79, 369, 623, 637
AflIII	ACRYGT	1 cut(s)	155
AgsI	TTSAA	1 cut(s)	269
AjnI	CCWGG	3 cut(s)	72, 451, 616
AluBI	AGCT	7 cut(s)	222, 401, 494, 548, 671, 825, 989
AluI	AGCT	7 cut(s)	222, 401, 494, 548, 671, 825, 989
Alw26I	GTCTC	1 cut(s)	318
AlwI	GGATC	3 cut(s)	448, 810, 830
Aor51HI	AGCGCT	1 cut(s)	1001
AoxI	GGCC	3 cut(s)	70, 457, 628
ApeKI	GCWGC	4 cut(s)	398, 410, 641, 986
ApoI	RAATTY	1 cut(s)	680
AseI	ATTAAT	2 cut(s)	333, 1011
Asp700I	GAANNNNTTC	1 cut(s)	683
AspA2I	CCTAGG	1 cut(s)	947
AspLEI	GCGC	1 cut(s)	1002
AspS9I	GGNCC	3 cut(s)	13, 59, 449
AsuHPI	GGTGA	1 cut(s)	254
AvaII	GGWCC	3 cut(s)	13, 59, 449
AvrII	CCTAGG	1 cut(s)	947
BalI	TGGCCA	1 cut(s)	72
BbvCI	CCTCAGC	1 cut(s)	909
BbvI	GCAGC	4 cut(s)	397, 410, 653, 998
BccI	CCATC	2 cut(s)	158, 589
BciT130I	CCWGG	3 cut(s)	74, 453, 618
BclI	TGATCA	1 cut(s)	696
BcoDI	GTCTC	1 cut(s)	318
BfaI	CTAG	4 cut(s)	437, 747, 948, 990
BfmI	CTRYAG	3 cut(s)	777, 811, 937
BfoI	RGCGCY	1 cut(s)	1003
BfuAI	ACCTGC	2 cut(s)	736, 775
BisI	GCNGC	6 cut(s)	55, 399, 411, 639, 642, 987
BlnI	CCTAGG	1 cut(s)	947
BlsI	GCNGC	6 cut(s)	56, 400, 412, 640, 643, 988
Bme1390I	CCNGG	3 cut(s)	74, 453, 618
Bme18I	GGWCC	3 cut(s)	13, 59, 449
BmgT120I	GGNCC	3 cut(s)	13, 59, 449
BmiI	GGNNCC	2 cut(s)	14, 432
BmrFI	CCNGG	3 cut(s)	74, 453, 618
BmrI	ACTGGG	1 cut(s)	426
BmuI	ACTGGG	1 cut(s)	426
BpmI	CTGGAG	4 cut(s)	28, 465, 645, 738
Bpu10I	CCTNAGC	1 cut(s)	909
BsaI	GGTCTC	1 cut(s)	318
BsaJI	CCNNGG	3 cut(s)	616, 740, 947
BsaXI	ACNNNNNCTCC	5 cut(s)	31, 145, 175, 877, 907
Bsc4I	CCNNNNNNNGG	6 cut(s)	28, 68, 79, 369, 623, 637
Bse118I	RCCGGY	1 cut(s)	309
Bse1I	ACTGG	5 cut(s)	385, 421, 585, 755, 917
BseBI	CCWGG	3 cut(s)	74, 453, 618
BseDI	CCNNGG	3 cut(s)	616, 740, 947
BseGI	GGATG	2 cut(s)	283, 581
BseLI	CCNNNNNNNGG	6 cut(s)	28, 68, 79, 369, 623, 637
BseMII	CTCAG	6 cut(s)	179, 240, 393, 597, 923, 984
BseNI	ACTGG	5 cut(s)	385, 421, 585, 755, 917
BseRI	GAGGAG	4 cut(s)	153, 382, 423, 852
BseXI	GCAGC	4 cut(s)	397, 410, 653, 998
BsgI	GTGCAG	3 cut(s)	714, 753, 1005
Bsh1236I	CGCG	1 cut(s)	157
BshFI	GGCC	3 cut(s)	72, 459, 630
BsiSI	CCGG	1 cut(s)	310
BsiWI	CGTACG	1 cut(s)	689
BslFI	GGGAC	4 cut(s)	26, 44, 433, 452
BslI	CCNNNNNNNGG	6 cut(s)	28, 68, 79, 369, 623, 637
BsmAI	GTCTC	1 cut(s)	318
BsmFI	GGGAC	4 cut(s)	26, 44, 433, 452
BsmI	GAATGC	2 cut(s)	311, 823
BsnI	GGCC	3 cut(s)	72, 459, 630
Bso31I	GGTCTC	1 cut(s)	318
Bsp1407I	TGTACA	1 cut(s)	179
Bsp143I	GATC	6 cut(s)	355, 440, 696, 802, 835, 992
Bsp19I	CCATGG	1 cut(s)	740
BspACI	CCGC	4 cut(s)	54, 57, 62, 638
BspANI	GGCC	3 cut(s)	72, 459, 630
BspCNI	CTCAG	6 cut(s)	178, 241, 394, 598, 922, 983
BspFNI	CGCG	1 cut(s)	157
BspHI	TCATGA	1 cut(s)	352
BspLI	GGNNCC	2 cut(s)	14, 432
BspMI	ACCTGC	2 cut(s)	736, 775
BspPI	GGATC	3 cut(s)	448, 810, 830
BspTNI	GGTCTC	1 cut(s)	318
BsrFI	RCCGGY	1 cut(s)	309
BsrGI	TGTACA	1 cut(s)	179
BsrI	ACTGG	5 cut(s)	385, 421, 585, 755, 917
BssAI	RCCGGY	1 cut(s)	309
BssECI	CCNNGG	3 cut(s)	616, 740, 947
BssMI	GATC	6 cut(s)	355, 440, 696, 802, 835, 992
BssT1I	CCWWGG	2 cut(s)	740, 947
Bst2UI	CCWGG	3 cut(s)	74, 453, 618
Bst4CI	ACNGT	7 cut(s)	17, 23, 50, 172, 184, 555, 778
Bst6I	CTCTTC	2 cut(s)	501, 870
BstAPI	GCANNNNNTGC	1 cut(s)	574
BstAUI	TGTACA	1 cut(s)	179
BstC8I	GCNNGC	3 cut(s)	823, 827, 831
BstDEI	CTNAG	6 cut(s)	165, 249, 402, 606, 909, 970
BstDSI	CCRYGG	1 cut(s)	740
BstF5I	GGATG	2 cut(s)	283, 581
BstFNI	CGCG	1 cut(s)	157
BstH2I	RGCGCY	1 cut(s)	1003
BstHHI	GCGC	1 cut(s)	1002
BstKTI	GATC	6 cut(s)	358, 443, 699, 805, 838, 995
BstMAI	GTCTC	1 cut(s)	318
BstMBI	GATC	6 cut(s)	355, 440, 696, 802, 835, 992
BstMWI	GCNNNNNNNGC	5 cut(s)	228, 407, 500, 574, 650
BstNI	CCWGG	3 cut(s)	74, 453, 618
BstNSI	RCATGY	1 cut(s)	856
BstSCI	CCNGG	3 cut(s)	72, 451, 616
BstSFI	CTRYAG	3 cut(s)	777, 811, 937
BstUI	CGCG	1 cut(s)	157
BstV1I	GCAGC	4 cut(s)	397, 410, 653, 998
BstX2I	RGATCY	1 cut(s)	440
BstXI	CCANNNNNNTGG	2 cut(s)	50, 335
BstYI	RGATCY	1 cut(s)	440
BsuRI	GGCC	3 cut(s)	72, 459, 630
BtgI	CCRYGG	1 cut(s)	740
BtsCI	GGATG	2 cut(s)	283, 581
BtsI	GCAGTG	2 cut(s)	532, 988
BtsIMutI	CAGTG	5 cut(s)	532, 560, 592, 599, 988
BveI	ACCTGC	2 cut(s)	736, 775
Cac8I	GCNNGC	3 cut(s)	823, 827, 831
CciI	TCATGA	1 cut(s)	352
CfoI	GCGC	1 cut(s)	1002
Cfr10I	RCCGGY	1 cut(s)	309
Cfr13I	GGNCC	3 cut(s)	13, 59, 449
CpoI	CGGWCCG	1 cut(s)	59
CseI	GACGC	2 cut(s)	146, 254
Csp6I	GTAC	5 cut(s)	180, 513, 690, 758, 847
CspI	CGGWCCG	1 cut(s)	59
CviAII	CATG	4 cut(s)	290, 353, 741, 853
CviQI	GTAC	5 cut(s)	180, 513, 690, 758, 847
DdeI	CTNAG	6 cut(s)	165, 249, 402, 606, 909, 970
DpnI	GATC	6 cut(s)	357, 442, 698, 804, 837, 994
DpnII	GATC	6 cut(s)	355, 440, 696, 802, 835, 992
EaeI	YGGCCR	1 cut(s)	70
Eam1104I	CTCTTC	2 cut(s)	501, 870
EarI	CTCTTC	2 cut(s)	501, 870
EciI	GGCGGA	1 cut(s)	51
Eco130I	CCWWGG	2 cut(s)	740, 947
Eco147I	AGGCCT	1 cut(s)	630
Eco31I	GGTCTC	1 cut(s)	318
Eco47I	GGWCC	3 cut(s)	13, 59, 449
Eco47III	AGCGCT	1 cut(s)	1001
Eco57I	CTGAAG	1 cut(s)	525
EcoO109I	RGGNCCY	1 cut(s)	449
EcoRII	CCWGG	3 cut(s)	72, 451, 616
EcoT14I	CCWWGG	2 cut(s)	740, 947
ErhI	CCWWGG	2 cut(s)	740, 947
FaeI	CATG	4 cut(s)	293, 356, 744, 856
FalI	AAGNNNNNCTT	2 cut(s)	561, 593
FaqI	GGGAC	4 cut(s)	26, 44, 433, 452
FatI	CATG	4 cut(s)	289, 352, 740, 852
FbaI	TGATCA	1 cut(s)	696
Fnu4HI	GCNGC	6 cut(s)	55, 399, 411, 639, 642, 987
FokI	GGATG	2 cut(s)	290, 568
Fsp4HI	GCNGC	6 cut(s)	55, 399, 411, 639, 642, 987
FspBI	CTAG	4 cut(s)	437, 747, 948, 990
GlaI	GCGC	1 cut(s)	1001
GluI	GCNGC	6 cut(s)	55, 399, 411, 639, 642, 987
GsuI	CTGGAG	4 cut(s)	28, 465, 645, 738
HaeII	RGCGCY	1 cut(s)	1003
HaeIII	GGCC	3 cut(s)	72, 459, 630
HapII	CCGG	1 cut(s)	310
HgaI	GACGC	2 cut(s)	146, 254
HhaI	GCGC	1 cut(s)	1002
Hin1II	CATG	4 cut(s)	293, 356, 744, 856
Hin6I	GCGC	1 cut(s)	1000
HinP1I	GCGC	1 cut(s)	1000
HinfI	GANTC	2 cut(s)	98, 736
HpaII	CCGG	1 cut(s)	310
HphI	GGTGA	1 cut(s)	254
Hpy166II	GTNNAC	1 cut(s)	180
Hpy188I	TCNGA	7 cut(s)	132, 168, 718, 885, 931, 973, 1006
Hpy188III	TCNNGA	4 cut(s)	7, 285, 353, 444
Hpy8I	GTNNAC	1 cut(s)	180
HpyAV	CCTTC	1 cut(s)	470
HpyCH4III	ACNGT	7 cut(s)	17, 23, 50, 172, 184, 555, 778
HpyCH4IV	ACGT	1 cut(s)	692
HpyCH4V	TGCA	4 cut(s)	413, 731, 770, 986
HpyF10VI	GCNNNNNNNGC	5 cut(s)	228, 407, 500, 574, 650
HpyF3I	CTNAG	6 cut(s)	165, 249, 402, 606, 909, 970
HpySE526I	ACGT	1 cut(s)	692
Hsp92II	CATG	4 cut(s)	293, 356, 744, 856
HspAI	GCGC	1 cut(s)	1000
Ksp22I	TGATCA	1 cut(s)	696
Kzo9I	GATC	6 cut(s)	355, 440, 696, 802, 835, 992
LmnI	GCTCC	3 cut(s)	436, 861, 1007
Lsp1109I	GCAGC	4 cut(s)	397, 410, 653, 998
MaeI	CTAG	4 cut(s)	437, 747, 948, 990
MaeII	ACGT	1 cut(s)	692
MaeIII	GTNAC	5 cut(s)	17, 242, 485, 794, 965
MalI	GATC	6 cut(s)	357, 442, 698, 804, 837, 994
MboI	GATC	6 cut(s)	355, 440, 696, 802, 835, 992
MboII	GAAGA	4 cut(s)	138, 518, 717, 857
MflI	RGATCY	1 cut(s)	440
MlsI	TGGCCA	1 cut(s)	72
MluCI	AATT	3 cut(s)	330, 680, 787
MluI	ACGCGT	1 cut(s)	155
MluNI	TGGCCA	1 cut(s)	72
MlyI	GAGTC	1 cut(s)	92
MmeI	TCCRAC	1 cut(s)	88
Mox20I	TGGCCA	1 cut(s)	72
MroXI	GAANNNNTTC	1 cut(s)	683
MscI	TGGCCA	1 cut(s)	72
MseI	TTAA	3 cut(s)	237, 333, 1011
Msp20I	TGGCCA	1 cut(s)	72
MspA1I	CMGCKG	1 cut(s)	401
MspI	CCGG	1 cut(s)	310
MspR9I	CCNGG	3 cut(s)	74, 453, 618
Mva1269I	GAATGC	2 cut(s)	311, 823
MvaI	CCWGG	3 cut(s)	74, 453, 618
MvnI	CGCG	1 cut(s)	157
MwoI	GCNNNNNNNGC	5 cut(s)	228, 407, 500, 574, 650
NcoI	CCATGG	1 cut(s)	740
NdeII	GATC	6 cut(s)	355, 440, 696, 802, 835, 992
NlaIII	CATG	4 cut(s)	293, 356, 744, 856
NlaIV	GGNNCC	2 cut(s)	14, 432
NmuCI	GTSAC	3 cut(s)	17, 242, 794
NspI	RCATGY	1 cut(s)	856
PagI	TCATGA	1 cut(s)	352
PceI	AGGCCT	1 cut(s)	630
PctI	GAATGC	2 cut(s)	311, 823
PdmI	GAANNNNTTC	1 cut(s)	683
PfeI	GAWTC	1 cut(s)	736
Pfl23II	CGTACG	1 cut(s)	689
PkrI	GCNGC	6 cut(s)	56, 400, 412, 640, 643, 988
PleI	GAGTC	1 cut(s)	92
PpsI	GAGTC	1 cut(s)	92
PpuMI	RGGWCCY	1 cut(s)	449
PshBI	ATTAAT	2 cut(s)	333, 1011
Psp5II	RGGWCCY	1 cut(s)	449
Psp6I	CCWGG	3 cut(s)	72, 451, 616
PspGI	CCWGG	3 cut(s)	72, 451, 616
PspLI	CGTACG	1 cut(s)	689
PspN4I	GGNNCC	2 cut(s)	14, 432
PspPI	GGNCC	3 cut(s)	13, 59, 449
PspPPI	RGGWCCY	1 cut(s)	449
PsrI	GAACNNNNNNTAC	2 cut(s)	333, 365
PsuI	RGATCY	1 cut(s)	440
PvuII	CAGCTG	1 cut(s)	401
RsaI	GTAC	5 cut(s)	181, 514, 691, 759, 848
RsaNI	GTAC	5 cut(s)	180, 513, 690, 758, 847
Rsr2I	CGGWCCG	1 cut(s)	59
RsrII	CGGWCCG	1 cut(s)	59
SaqAI	TTAA	3 cut(s)	237, 333, 1011
SatI	GCNGC	6 cut(s)	55, 399, 411, 639, 642, 987
Sau3AI	GATC	6 cut(s)	355, 440, 696, 802, 835, 992
Sau96I	GGNCC	3 cut(s)	13, 59, 449
SchI	GAGTC	1 cut(s)	92
ScrFI	CCNGG	3 cut(s)	74, 453, 618
SfcI	CTRYAG	3 cut(s)	777, 811, 937
SinI	GGWCC	3 cut(s)	13, 59, 449
Sse9I	AATT	3 cut(s)	330, 680, 787
SseBI	AGGCCT	1 cut(s)	630
SsiI	CCGC	4 cut(s)	54, 57, 62, 638
SspMI	CTAG	4 cut(s)	437, 747, 948, 990
StuI	AGGCCT	1 cut(s)	630
StyD4I	CCNGG	3 cut(s)	72, 451, 616
StyI	CCWWGG	2 cut(s)	740, 947
TaaI	ACNGT	7 cut(s)	17, 23, 50, 172, 184, 555, 778
TaiI	ACGT	1 cut(s)	695
TaqI	TCGA	1 cut(s)	550
TasI	AATT	3 cut(s)	330, 680, 787
TatI	WGTACW	3 cut(s)	179, 757, 846
TauI	GCSGC	2 cut(s)	57, 641
TfiI	GAWTC	1 cut(s)	736
Tru1I	TTAA	3 cut(s)	237, 333, 1011
Tru9I	TTAA	3 cut(s)	237, 333, 1011
TscAI	CASTG	5 cut(s)	532, 560, 592, 606, 988
TseFI	GTSAC	3 cut(s)	17, 242, 794
TseI	GCWGC	4 cut(s)	398, 410, 641, 986
Tsp45I	GTSAC	3 cut(s)	17, 242, 794
TspDTI	ATGAA	4 cut(s)	116, 341, 692, 798
TspGWI	ACGGA	1 cut(s)	677
TspRI	CASTG	5 cut(s)	532, 560, 592, 606, 988
VpaK11BI	GGWCC	3 cut(s)	13, 59, 449
VspI	ATTAAT	2 cut(s)	333, 1011
XapI	RAATTY	1 cut(s)	680
XceI	RCATGY	1 cut(s)	856
XmaJI	CCTAGG	1 cut(s)	947
XmnI	GAANNNNTTC	1 cut(s)	683
XspI	CTAG	4 cut(s)	437, 747, 948, 990

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.