MD09G1003900.v1.1

Enoyl-(Acyl carrier protein) reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012770
Physical Location & Seq
Reverse (-)
340975 .. 343728
2754 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1003900.v1.1.491

Sequence Viewer

Length: 912 bp
ATGATCAGATCACTAACCAGAGAGTTTAAGCTGATCAGCTGCAATGCTTTTCTCTGTAAGCGAGCAAGGCCCTTTTCAACGAGAGGCGCAGCTGGCCATGGAAGGCTGCAAGGCAAGGTAGCCATAATAACAGGGTCAGCTAGCGGGCTTGGGAAAGCCACGGCCCACGAGTTCATCCGACATGGAGCACACGTCATCGTTGCAGACTCAGATTCTGAGCTGGGTCCACAAGTCGCCAAAGAGCTGGGGCCCGCAGCCCACTTTGTCCAATGCGACGTTGCCGTTGAGTCCCAAATAGCAGAAGCTGTAGAAACCGCCATGGCTCGTCACGGAAAACTTGACATAATGTACAACAACGCGGGAATAACAGGCCCAGCGTTCCCGCCCAGCATCGTTGACCTGAACCTTGAGGAGTTTGACCGCGTGATCCGGGTCAACGTGCGGGGCGCGGTTGCTGGGATAAAGCATGCGGCACGAGTCATGATACCTGCGGGCTCAGGCTCCATACTGTGCACATCCAGCATAGCTGGGCTGTTGGGTGGGGTTGGGCCTCACCCCTATTCGATATCGAAATTTACGATACCTGGCCTGGTCAAGTCACTGGCGAGCGAGCTGTGCCGAAACGGGATCCGAGTCAATTGCATTTCGCCAAGTCCAATCCCGACGTCAATGGTGGTGCGGGAAATATGGAAGCTTTATCCGGGGGCGCCGGAGGATCAGGTTAAAGAGATAGTGAATGGGCTGGGAGAGCTCAAGGGTGCGAAGTGTGAGGAGATAGATGTGGCGCAAGCTGCGGTGTATCTGGCATCGGATGAAGCAAAGTATGTGACGGGTCATAATCTGGTGGTCGATGGAGGCTTCACCAGCTTCAAAAGTCTTAGCTTCCCTCCTCCTCCGGGTCAATCTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

304

Amino Acids

31.87

Weight (kDa)

8.44

Isoelectric Point (pI)

30.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 39 - 225 3.6e-49 short chain dehydrogenase
KR PF08659 42 - 200 3.1e-12 KR domain
adh_short_C2 PF13561 45 - 288 4.6e-59 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 668
Acc36I ACCTGC 1 cut(s) 496
AccB1I GGYRCC 1 cut(s) 706
AccII CGCG 3 cut(s) 359, 423, 449
AclWI GGATC 4 cut(s) 421, 622, 635, 723
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 1 cut(s) 572
AcyI GRCGYC 2 cut(s) 665, 707
AfaI GTAC 1 cut(s) 350
AfiI CCNNNNNNNGG 1 cut(s) 896
AflIII ACRYGT 1 cut(s) 190
AgsI TTSAA 2 cut(s) 78, 871
AjiI CACGTC 1 cut(s) 193
AjnI CCWGG 2 cut(s) 583, 588
Alw21I GWGCWC 3 cut(s) 190, 515, 753
Alw44I GTGCAC 1 cut(s) 511
AlwI GGATC 4 cut(s) 421, 622, 635, 723
AlwNI CAGNNNCTG 2 cut(s) 215, 305
AoxI GGCC 7 cut(s) 68, 94, 162, 248, 370, 548, 586
ApaI GGGCCC 1 cut(s) 252
ApaLI GTGCAC 1 cut(s) 511
ApeKI GCWGC 5 cut(s) 39, 89, 106, 254, 791
ApoI RAATTY 1 cut(s) 572
AspLEI GCGC 4 cut(s) 89, 449, 709, 787
AspS9I GGNCC 7 cut(s) 69, 163, 224, 248, 249, 371, 548
AsuC2I CCSGG 3 cut(s) 431, 702, 897
AsuHPI GGTGA 2 cut(s) 545, 853
AsuNHI GCTAGC 1 cut(s) 140
AvaII GGWCC 1 cut(s) 224
BaeGI GKGCMC 2 cut(s) 252, 515
BalI TGGCCA 1 cut(s) 96
BamHI GGATCC 1 cut(s) 627
BanI GGYRCC 1 cut(s) 706
BanII GRGCYC 3 cut(s) 252, 497, 753
BauI CACGAG 2 cut(s) 167, 474
Bbv12I GWGCWC 3 cut(s) 190, 515, 753
BbvI GCAGC 5 cut(s) 26, 93, 101, 266, 778
BccI CCATC 1 cut(s) 845
BceAI ACGGC 2 cut(s) 177, 266
BcgI CGANNNNNNTGC 2 cut(s) 621, 655
BciT130I CCWGG 2 cut(s) 585, 590
BclI TGATCA 2 cut(s) 3, 33
BcnI CCSGG 3 cut(s) 431, 702, 897
BfaI CTAG 1 cut(s) 141
BfmI CTRYAG 1 cut(s) 306
BfoI RGCGCY 1 cut(s) 710
BfuAI ACCTGC 1 cut(s) 496
BisI GCNGC 6 cut(s) 40, 90, 107, 255, 471, 792
BlsI GCNGC 6 cut(s) 41, 91, 108, 256, 472, 793
Bme1390I CCNGG 5 cut(s) 431, 585, 590, 702, 897
Bme18I GGWCC 1 cut(s) 224
BmgBI CACGTC 1 cut(s) 193
BmgT120I GGNCC 7 cut(s) 69, 163, 224, 248, 249, 371, 548
BmiI GGNNCC 6 cut(s) 225, 249, 250, 502, 629, 708
BmrFI CCNGG 5 cut(s) 431, 585, 590, 702, 897
BmsI GCATC 2 cut(s) 399, 815
BmtI GCTAGC 1 cut(s) 144
Bpu10I CCTNAGC 1 cut(s) 496
BpuEI CTTGAG 2 cut(s) 428, 737
BpuMI CCSGG 3 cut(s) 431, 702, 897
BsaHI GRCGYC 2 cut(s) 665, 707
BsaJI CCNNGG 4 cut(s) 97, 159, 318, 701
BsaXI ACNNNNNCTCC 2 cut(s) 177, 207
Bsc4I CCNNNNNNNGG 1 cut(s) 896
Bse1I ACTGG 1 cut(s) 606
Bse3DI GCAATG 1 cut(s) 49
BseBI CCWGG 2 cut(s) 585, 590
BseDI CCNNGG 4 cut(s) 97, 159, 318, 701
BseGI GGATG 3 cut(s) 174, 515, 817
BseLI CCNNNNNNNGG 1 cut(s) 896
BseMI GCAATG 1 cut(s) 49
BseMII CTCAG 3 cut(s) 207, 222, 510
BseNI ACTGG 1 cut(s) 606
BseRI GAGGAG 4 cut(s) 425, 785, 879, 882
BseSI GKGCMC 2 cut(s) 252, 515
BseXI GCAGC 5 cut(s) 26, 93, 101, 266, 778
BseYI CCCAGC 7 cut(s) 220, 244, 373, 386, 455, 527, 742
Bsh1236I CGCG 3 cut(s) 359, 423, 449
BshFI GGCC 7 cut(s) 70, 96, 164, 250, 372, 550, 588
BshNI GGYRCC 1 cut(s) 706
BsiHKAI GWGCWC 3 cut(s) 190, 515, 753
BsiSI CCGG 4 cut(s) 430, 701, 710, 896
BslFI GGGAC 1 cut(s) 274
BslI CCNNNNNNNGG 1 cut(s) 896
BsmFI GGGAC 1 cut(s) 274
BsnI GGCC 7 cut(s) 70, 96, 164, 250, 372, 550, 588
Bsp120I GGGCCC 1 cut(s) 248
Bsp1286I GDGCHC 5 cut(s) 190, 252, 497, 515, 753
Bsp1407I TGTACA 1 cut(s) 348
Bsp143I GATC 6 cut(s) 3, 8, 33, 426, 627, 715
Bsp19I CCATGG 2 cut(s) 97, 318
BspANI GGCC 7 cut(s) 70, 96, 164, 250, 372, 550, 588
BspCNI CTCAG 3 cut(s) 208, 221, 509
BspFNI CGCG 3 cut(s) 359, 423, 449
BspHI TCATGA 1 cut(s) 480
BspLI GGNNCC 6 cut(s) 225, 249, 250, 502, 629, 708
BspMI ACCTGC 1 cut(s) 496
BspOI GCTAGC 1 cut(s) 144
BspPI GGATC 4 cut(s) 421, 622, 635, 723
BspT107I GGYRCC 1 cut(s) 706
BsrDI GCAATG 1 cut(s) 49
BsrGI TGTACA 1 cut(s) 348
BsrI ACTGG 1 cut(s) 606
BssECI CCNNGG 4 cut(s) 97, 159, 318, 701
BssMI GATC 6 cut(s) 3, 8, 33, 426, 627, 715
BssNI GRCGYC 2 cut(s) 665, 707
BssSI CACGAG 2 cut(s) 167, 474
BssT1I CCWWGG 2 cut(s) 97, 318
Bst2BI CACGAG 2 cut(s) 167, 474
Bst2UI CCWGG 2 cut(s) 585, 590
Bst4CI ACNGT 1 cut(s) 510
BstACI GRCGYC 2 cut(s) 665, 707
BstAUI TGTACA 1 cut(s) 348
BstDEI CTNAG 4 cut(s) 208, 216, 496, 878
BstDSI CCRYGG 3 cut(s) 97, 159, 318
BstF5I GGATG 3 cut(s) 174, 515, 817
BstFNI CGCG 3 cut(s) 359, 423, 449
BstH2I RGCGCY 1 cut(s) 710
BstHHI GCGC 4 cut(s) 89, 449, 709, 787
BstKTI GATC 6 cut(s) 6, 11, 36, 429, 630, 718
BstMBI GATC 6 cut(s) 3, 8, 33, 426, 627, 715
BstMWI GCNNNNNNNGC 7 cut(s) 67, 93, 519, 615, 748, 791, 864
BstNI CCWGG 2 cut(s) 585, 590
BstNSI RCATGY 1 cut(s) 470
BstSCI CCNGG 5 cut(s) 429, 583, 588, 700, 895
BstSFI CTRYAG 1 cut(s) 306
BstSLI GKGCMC 2 cut(s) 252, 515
BstUI CGCG 3 cut(s) 359, 423, 449
BstV1I GCAGC 5 cut(s) 26, 93, 101, 266, 778
BstX2I RGATCY 1 cut(s) 627
BstXI CCANNNNNNTGG 1 cut(s) 244
BstYI RGATCY 1 cut(s) 627
BsuRI GGCC 7 cut(s) 70, 96, 164, 250, 372, 550, 588
BtgI CCRYGG 3 cut(s) 97, 159, 318
BtrI CACGTC 1 cut(s) 193
BtsCI GGATG 3 cut(s) 174, 515, 817
BtsIMutI CAGTG 1 cut(s) 599
BveI ACCTGC 1 cut(s) 496
CaiI CAGNNNCTG 2 cut(s) 215, 305
CciI TCATGA 1 cut(s) 480
CfoI GCGC 4 cut(s) 89, 449, 709, 787
Cfr13I GGNCC 7 cut(s) 69, 163, 224, 248, 249, 371, 548
Csp6I GTAC 1 cut(s) 349
CviAII CATG 5 cut(s) 98, 182, 319, 467, 481
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 4 cut(s) 208, 216, 496, 878
DinI GGCGCC 1 cut(s) 708
DpnI GATC 6 cut(s) 5, 10, 35, 428, 629, 717
DpnII GATC 6 cut(s) 3, 8, 33, 426, 627, 715
EaeI YGGCCR 1 cut(s) 94
Ecl136II GAGCTC 1 cut(s) 751
Eco130I CCWWGG 2 cut(s) 97, 318
Eco24I GRGCYC 3 cut(s) 252, 497, 753
Eco32I GATATC 1 cut(s) 567
Eco47I GGWCC 1 cut(s) 224
Eco53kI GAGCTC 1 cut(s) 751
EcoICRI GAGCTC 1 cut(s) 751
EcoO109I RGGNCCY 2 cut(s) 69, 248
EcoRII CCWGG 2 cut(s) 583, 588
EcoRV GATATC 1 cut(s) 567
EcoT14I CCWWGG 2 cut(s) 97, 318
EcoT38I GRGCYC 3 cut(s) 252, 497, 753
EgeI GGCGCC 1 cut(s) 708
EheI GGCGCC 1 cut(s) 708
ErhI CCWWGG 2 cut(s) 97, 318
FaeI CATG 5 cut(s) 101, 185, 322, 470, 484
FaqI GGGAC 1 cut(s) 274
FatI CATG 5 cut(s) 97, 181, 318, 466, 480
FauI CCCGC 7 cut(s) 137, 259, 352, 390, 435, 484, 672
FbaI TGATCA 2 cut(s) 3, 33
Fnu4HI GCNGC 6 cut(s) 40, 90, 107, 255, 471, 792
FokI GGATG 3 cut(s) 161, 502, 824
FriOI GRGCYC 3 cut(s) 252, 497, 753
Fsp4HI GCNGC 6 cut(s) 40, 90, 107, 255, 471, 792
FspBI CTAG 1 cut(s) 141
GlaI GCGC 4 cut(s) 88, 448, 708, 786
GluI GCNGC 6 cut(s) 40, 90, 107, 255, 471, 792
GsaI CCCAGC 7 cut(s) 224, 248, 377, 390, 459, 531, 746
HaeII RGCGCY 1 cut(s) 710
HaeIII GGCC 7 cut(s) 70, 96, 164, 250, 372, 550, 588
HapII CCGG 4 cut(s) 430, 701, 710, 896
HhaI GCGC 4 cut(s) 89, 449, 709, 787
Hin1I GRCGYC 2 cut(s) 665, 707
Hin1II CATG 5 cut(s) 101, 185, 322, 470, 484
Hin6I GCGC 4 cut(s) 87, 447, 707, 785
HinP1I GCGC 4 cut(s) 87, 447, 707, 785
HincII GTYRAC 2 cut(s) 397, 436
HindII GTYRAC 2 cut(s) 397, 436
HindIII AAGCTT 1 cut(s) 692
HinfI GANTC 5 cut(s) 206, 212, 287, 477, 633
HpaII CCGG 4 cut(s) 430, 701, 710, 896
HphI GGTGA 2 cut(s) 545, 853
Hpy166II GTNNAC 4 cut(s) 227, 397, 436, 513
Hpy188I TCNGA 6 cut(s) 8, 179, 211, 217, 632, 811
Hpy188III TCNNGA 2 cut(s) 481, 661
Hpy8I GTNNAC 4 cut(s) 227, 397, 436, 513
Hpy99I CGWCG 2 cut(s) 278, 667
HpyAV CCTTC 1 cut(s) 96
HpyCH4III ACNGT 1 cut(s) 510
HpyCH4IV ACGT 4 cut(s) 192, 276, 438, 665
HpyCH4V TGCA 5 cut(s) 42, 109, 203, 513, 642
HpyF10VI GCNNNNNNNGC 7 cut(s) 67, 93, 519, 615, 748, 791, 864
HpyF3I CTNAG 4 cut(s) 208, 216, 496, 878
HpySE526I ACGT 4 cut(s) 192, 276, 438, 665
Hsp92I GRCGYC 2 cut(s) 665, 707
Hsp92II CATG 5 cut(s) 101, 185, 322, 470, 484
HspAI GCGC 4 cut(s) 87, 447, 707, 785
KasI GGCGCC 1 cut(s) 706
Ksp22I TGATCA 2 cut(s) 3, 33
Kzo9I GATC 6 cut(s) 3, 8, 33, 426, 627, 715
LmnI GCTCC 2 cut(s) 185, 506
Lsp1109I GCAGC 5 cut(s) 26, 93, 101, 266, 778
LweI GCATC 2 cut(s) 399, 815
MaeI CTAG 1 cut(s) 141
MaeII ACGT 4 cut(s) 192, 276, 438, 665
MaeIII GTNAC 3 cut(s) 326, 597, 826
MalI GATC 6 cut(s) 5, 10, 35, 428, 629, 717
MboI GATC 6 cut(s) 3, 8, 33, 426, 627, 715
MfeI CAATTG 1 cut(s) 637
MflI RGATCY 1 cut(s) 627
MhlI GDGCHC 5 cut(s) 190, 252, 497, 515, 753
MlsI TGGCCA 1 cut(s) 96
MluCI AATT 2 cut(s) 572, 637
MluNI TGGCCA 1 cut(s) 96
Mly113I GGCGCC 1 cut(s) 707
MlyI GAGTC 4 cut(s) 200, 296, 486, 642
MmeI TCCRAC 1 cut(s) 202
MnlI CCTC 9 cut(s) 77, 403, 561, 706, 763, 848, 897, 900, 903
Mox20I TGGCCA 1 cut(s) 96
MscI TGGCCA 1 cut(s) 96
MseI TTAA 2 cut(s) 27, 723
Msp20I TGGCCA 1 cut(s) 96
MspA1I CMGCKG 2 cut(s) 39, 92
MspI CCGG 4 cut(s) 430, 701, 710, 896
MspR9I CCNGG 5 cut(s) 431, 585, 590, 702, 897
MunI CAATTG 1 cut(s) 637
MvaI CCWGG 2 cut(s) 585, 590
MvnI CGCG 3 cut(s) 359, 423, 449
MwoI GCNNNNNNNGC 7 cut(s) 67, 93, 519, 615, 748, 791, 864
NarI GGCGCC 1 cut(s) 707
NciI CCSGG 3 cut(s) 431, 702, 897
NcoI CCATGG 2 cut(s) 97, 318
NdeII GATC 6 cut(s) 3, 8, 33, 426, 627, 715
NheI GCTAGC 1 cut(s) 140
NlaIII CATG 5 cut(s) 101, 185, 322, 470, 484
NlaIV GGNNCC 6 cut(s) 225, 249, 250, 502, 629, 708
NmuCI GTSAC 3 cut(s) 326, 597, 826
NspI RCATGY 1 cut(s) 470
PaeI GCATGC 1 cut(s) 470
PagI TCATGA 1 cut(s) 480
PcsI WCGNNNNNNNCGW 1 cut(s) 575
PfeI GAWTC 1 cut(s) 212
PkrI GCNGC 6 cut(s) 41, 91, 108, 256, 472, 793
PleI GAGTC 4 cut(s) 200, 295, 485, 641
PluTI GGCGCC 1 cut(s) 710
PpsI GAGTC 4 cut(s) 200, 295, 485, 641
Psp124BI GAGCTC 1 cut(s) 753
Psp6I CCWGG 2 cut(s) 583, 588
PspFI CCCAGC 7 cut(s) 220, 244, 373, 386, 455, 527, 742
PspGI CCWGG 2 cut(s) 583, 588
PspN4I GGNNCC 6 cut(s) 225, 249, 250, 502, 629, 708
PspOMI GGGCCC 1 cut(s) 248
PspPI GGNCC 7 cut(s) 69, 163, 224, 248, 249, 371, 548
PstNI CAGNNNCTG 2 cut(s) 215, 305
PsuI RGATCY 1 cut(s) 627
PvuII CAGCTG 2 cut(s) 39, 92
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
SacI GAGCTC 1 cut(s) 753
SaqAI TTAA 2 cut(s) 27, 723
SatI GCNGC 6 cut(s) 40, 90, 107, 255, 471, 792
Sau3AI GATC 6 cut(s) 3, 8, 33, 426, 627, 715
Sau96I GGNCC 7 cut(s) 69, 163, 224, 248, 249, 371, 548
SchI GAGTC 4 cut(s) 200, 296, 486, 642
ScrFI CCNGG 5 cut(s) 431, 585, 590, 702, 897
SduI GDGCHC 5 cut(s) 190, 252, 497, 515, 753
SfaNI GCATC 2 cut(s) 399, 815
SfcI CTRYAG 1 cut(s) 306
SfoI GGCGCC 1 cut(s) 708
SinI GGWCC 1 cut(s) 224
SmlI CTYRAG 2 cut(s) 407, 752
SmoI CTYRAG 2 cut(s) 407, 752
SphI GCATGC 1 cut(s) 470
Sse9I AATT 2 cut(s) 572, 637
SspDI GGCGCC 1 cut(s) 706
SspMI CTAG 1 cut(s) 141
SstI GAGCTC 1 cut(s) 753
StyD4I CCNGG 5 cut(s) 429, 583, 588, 700, 895
StyI CCWWGG 2 cut(s) 97, 318
TaaI ACNGT 1 cut(s) 510
TaiI ACGT 4 cut(s) 195, 279, 441, 668
TaqI TCGA 3 cut(s) 563, 569, 849
TasI AATT 2 cut(s) 572, 637
TatI WGTACW 1 cut(s) 348
TauI GCSGC 1 cut(s) 473
TfiI GAWTC 1 cut(s) 212
Tru1I TTAA 2 cut(s) 27, 723
Tru9I TTAA 2 cut(s) 27, 723
TscAI CASTG 1 cut(s) 606
TseFI GTSAC 3 cut(s) 326, 597, 826
TseI GCWGC 5 cut(s) 39, 89, 106, 254, 791
Tsp45I GTSAC 3 cut(s) 326, 597, 826
TspDTI ATGAA 2 cut(s) 163, 828
TspGWI ACGGA 1 cut(s) 345
TspRI CASTG 1 cut(s) 606
VneI GTGCAC 1 cut(s) 511
VpaK11BI GGWCC 1 cut(s) 224
XapI RAATTY 1 cut(s) 572
XceI RCATGY 1 cut(s) 470
XspI CTAG 1 cut(s) 141
ZraI GACGTC 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.