Rw2G055160

Enoyl-(Acyl carrier protein) reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012770
Physical Location & Seq
Forward (+)
82653962 .. 82655804
1843 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G055160.1

Sequence Viewer

Length: 942 bp
ATGCTCAGATCATTTGCCATCAGCAGATTCAAAGAGGCAGCTGGAGCAGCTAGAGGGCTCAAGTACTACGGCAATCATGTTCTCTGTAAGCGAACGAGACCTTATTCAACCGCTGGCCGTAGTGGAAATGGAAGGCTGGAAGGTAAGGTAGCCTTAATAACAGGGTCCGCAAGTGGGCTCGGGAAGGCAGCGGCCCACGAATTCATCCAAAATGGGGCGCATGTCATGATTGCAGACACTGATTGTGAGATGGGCCAACAAGTAGCTGAAGAGCTGGGCCCAACAGCCCACTTTGTGGAATGCGACGTTGCTGTTGAGTCACAAGTAGCACAAGCTGTAGAAACGACCGTGGAAAGGTACGGAAAGCTCGACATAATGTACAACAATGCTGGAATAACCGGGCCGACAATCCCACCCAGCATTGCCGACCTCGATCTCGATGAGTTTGACCGTGTCATGCGGATCAACGTGAGGGGAATAATTGCCGGGATAAAGCATGCTGCCCGAGTGATGATCCCGTTGGGTTCGGGGTCCATACTGTGCACATCTAGCATTACTGGGCTCCTGGGTGGGCTTGGGCCGCATCCTTACTCGATTTCCAAGTTTACAATACCAGGCATAGTGAAGTCTGTGGCAAGCGAGCTGTGCCGAAGTGGGGTTCGAATCAATTGCATTTCACCGGCTCCAATTCCAACGCCTCTTGTGGTTAGGGAGATAAGTCGGTTTTATCCAGGGGCAACGGAGGAGCAGATTATAGAGATAGTGAATGGTGTGGGAGAACTTAAGGGTGCAAAGGCGGAGGCAGTAGATGTAGCCAAGGCAGCGCTCTATTTGGCCTCTGATGAAGCAAAGTATGTAACAGGTCATAATCTAGTAGTCGATGGAGGCTTCACCTGCTTTAAAACTCTTAGCTTTCCTCATCCTGATCAAGTACTTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

313

Amino Acids

33.12

Weight (kDa)

6.71

Isoelectric Point (pI)

22.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 49 - 235 1.5e-46 short chain dehydrogenase
KR PF08659 51 - 200 8.1e-10 KR domain
adh_short_C2 PF13561 55 - 298 4.6e-57 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 902
Acc36I ACCTGC 1 cut(s) 902
AccB7I CCANNNNNTGG 1 cut(s) 295
AciI CCGC 6 cut(s) 111, 168, 191, 460, 581, 797
AclWI GGATC 2 cut(s) 470, 508
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 1 cut(s) 200
AcuI CTGAAG 1 cut(s) 288
AdeI CACNNNGTG 1 cut(s) 295
AfaI GTAC 4 cut(s) 65, 359, 380, 933
AfeI AGCGCT 1 cut(s) 825
AfiI CCNNNNNNNGG 5 cut(s) 174, 214, 295, 354, 655
AflII CTTAAG 1 cut(s) 782
AgsI TTSAA 2 cut(s) 31, 108
AjnI CCWGG 3 cut(s) 564, 613, 730
AluBI AGCT 8 cut(s) 41, 50, 266, 274, 335, 367, 643, 912
AluI AGCT 8 cut(s) 41, 50, 266, 274, 335, 367, 643, 912
Alw21I GWGCWC 1 cut(s) 545
Alw26I GTCTC 1 cut(s) 91
Alw44I GTGCAC 1 cut(s) 541
AlwI GGATC 2 cut(s) 470, 508
AlwNI CAGNNNCTG 1 cut(s) 239
Ama87I CYCGRG 2 cut(s) 179, 504
Aor51HI AGCGCT 1 cut(s) 825
AoxI GGCC 7 cut(s) 115, 192, 253, 277, 401, 578, 834
ApaI GGGCCC 1 cut(s) 281
ApaLI GTGCAC 1 cut(s) 541
ApeKI GCWGC 5 cut(s) 38, 47, 188, 500, 821
ApoI RAATTY 1 cut(s) 200
AspLEI GCGC 2 cut(s) 220, 826
AspS9I GGNCC 8 cut(s) 165, 193, 253, 277, 278, 401, 531, 578
AsuC2I CCSGG 2 cut(s) 400, 487
AsuHPI GGTGA 2 cut(s) 669, 883
AsuII TTCGAA 1 cut(s) 661
AvaI CYCGRG 2 cut(s) 179, 504
AvaII GGWCC 2 cut(s) 165, 531
BaeGI GKGCMC 2 cut(s) 281, 545
BanII GRGCYC 4 cut(s) 60, 180, 281, 564
Bbv12I GWGCWC 1 cut(s) 545
BbvI GCAGC 5 cut(s) 50, 59, 200, 487, 833
BccI CCATC 3 cut(s) 26, 244, 875
BceAI ACGGC 2 cut(s) 85, 102
BcgI CGANNNNNNTGC 2 cut(s) 651, 685
BciT130I CCWGG 3 cut(s) 566, 615, 732
BclI TGATCA 1 cut(s) 925
BcnI CCSGG 2 cut(s) 400, 487
BcoDI GTCTC 1 cut(s) 91
BfaI CTAG 3 cut(s) 51, 549, 872
BfmI CTRYAG 1 cut(s) 336
BfoI RGCGCY 1 cut(s) 827
BfrI CTTAAG 1 cut(s) 782
BfuAI ACCTGC 1 cut(s) 902
BisI GCNGC 7 cut(s) 39, 48, 189, 192, 501, 581, 822
BlsI GCNGC 7 cut(s) 40, 49, 190, 193, 502, 582, 823
BmcAI AGTACT 2 cut(s) 65, 933
Bme1390I CCNGG 5 cut(s) 400, 487, 566, 615, 732
Bme18I GGWCC 2 cut(s) 165, 531
BmeT110I CYCGRG 2 cut(s) 179, 504
BmgT120I GGNCC 8 cut(s) 165, 193, 253, 277, 278, 401, 531, 578
BmiI GGNNCC 5 cut(s) 166, 279, 532, 563, 684
BmrFI CCNGG 5 cut(s) 400, 487, 566, 615, 732
BmrI ACTGGG 1 cut(s) 567
BmsI GCATC 1 cut(s) 592
BmuI ACTGGG 1 cut(s) 567
BpmI CTGGAG 1 cut(s) 63
Bpu14I TTCGAA 1 cut(s) 661
BpuEI CTTGAG 1 cut(s) 44
BpuMI CCSGG 2 cut(s) 400, 487
BsaI GGTCTC 1 cut(s) 91
BsaJI CCNNGG 4 cut(s) 348, 565, 731, 816
Bsc4I CCNNNNNNNGG 5 cut(s) 174, 214, 295, 354, 655
Bse118I RCCGGY 1 cut(s) 679
Bse1I ACTGG 1 cut(s) 562
Bse3DI GCAATG 1 cut(s) 420
BseBI CCWGG 3 cut(s) 566, 615, 732
BseDI CCNNGG 4 cut(s) 348, 565, 731, 816
BseGI GGATG 3 cut(s) 204, 583, 919
BseLI CCNNNNNNNGG 5 cut(s) 174, 214, 295, 354, 655
BseMI GCAATG 1 cut(s) 420
BseMII CTCAG 1 cut(s) 19
BseNI ACTGG 1 cut(s) 562
BseRI GAGGAG 1 cut(s) 758
BseSI GKGCMC 2 cut(s) 281, 545
BseXI GCAGC 5 cut(s) 50, 59, 200, 487, 833
BseYI CCCAGC 2 cut(s) 274, 416
Bsh1285I CGRYCG 1 cut(s) 348
BshFI GGCC 7 cut(s) 117, 194, 255, 279, 403, 580, 836
BsiEI CGRYCG 1 cut(s) 348
BsiHKAI GWGCWC 1 cut(s) 545
BsiHKCI CYCGRG 2 cut(s) 179, 504
BsiSI CCGG 3 cut(s) 399, 486, 680
BslI CCNNNNNNNGG 5 cut(s) 174, 214, 295, 354, 655
BsmAI GTCTC 1 cut(s) 91
BsmI GAATGC 1 cut(s) 305
BsnI GGCC 7 cut(s) 117, 194, 255, 279, 403, 580, 836
Bso31I GGTCTC 1 cut(s) 91
BsoBI CYCGRG 2 cut(s) 179, 504
Bsp119I TTCGAA 1 cut(s) 661
Bsp120I GGGCCC 1 cut(s) 277
Bsp1286I GDGCHC 5 cut(s) 60, 180, 281, 545, 564
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 5 cut(s) 8, 433, 462, 513, 925
BspACI CCGC 6 cut(s) 111, 168, 191, 460, 581, 797
BspANI GGCC 7 cut(s) 117, 194, 255, 279, 403, 580, 836
BspCNI CTCAG 1 cut(s) 18
BspHI TCATGA 1 cut(s) 225
BspLI GGNNCC 5 cut(s) 166, 279, 532, 563, 684
BspMI ACCTGC 1 cut(s) 902
BspPI GGATC 2 cut(s) 470, 508
BspQI GCTCTTC 1 cut(s) 264
BspT104I TTCGAA 1 cut(s) 661
BspTI CTTAAG 1 cut(s) 782
BspTNI GGTCTC 1 cut(s) 91
BsrDI GCAATG 1 cut(s) 420
BsrFI RCCGGY 1 cut(s) 679
BsrGI TGTACA 1 cut(s) 378
BsrI ACTGG 1 cut(s) 562
BssAI RCCGGY 1 cut(s) 679
BssECI CCNNGG 4 cut(s) 348, 565, 731, 816
BssMI GATC 5 cut(s) 8, 433, 462, 513, 925
BssT1I CCWWGG 1 cut(s) 816
Bst2UI CCWGG 3 cut(s) 566, 615, 732
Bst4CI ACNGT 3 cut(s) 349, 452, 540
Bst6I CTCTTC 1 cut(s) 264
BstAFI CTTAAG 1 cut(s) 782
BstAUI TGTACA 1 cut(s) 378
BstBI TTCGAA 1 cut(s) 661
BstC8I GCNNGC 4 cut(s) 115, 498, 637, 641
BstDEI CTNAG 2 cut(s) 5, 908
BstDSI CCRYGG 1 cut(s) 348
BstF5I GGATG 3 cut(s) 204, 583, 919
BstH2I RGCGCY 1 cut(s) 827
BstHHI GCGC 2 cut(s) 220, 826
BstKTI GATC 5 cut(s) 11, 436, 465, 516, 928
BstMAI GTCTC 1 cut(s) 91
BstMBI GATC 5 cut(s) 8, 433, 462, 513, 925
BstMCI CGRYCG 1 cut(s) 348
BstMWI GCNNNNNNNGC 7 cut(s) 44, 47, 549, 580, 645, 821, 894
BstNI CCWGG 3 cut(s) 566, 615, 732
BstNSI RCATGY 2 cut(s) 224, 500
BstSCI CCNGG 5 cut(s) 398, 485, 564, 613, 730
BstSFI CTRYAG 1 cut(s) 336
BstSLI GKGCMC 2 cut(s) 281, 545
BstV1I GCAGC 5 cut(s) 50, 59, 200, 487, 833
BsuRI GGCC 7 cut(s) 117, 194, 255, 279, 403, 580, 836
BtgI CCRYGG 1 cut(s) 348
BtsCI GGATG 3 cut(s) 204, 583, 919
BtsIMutI CAGTG 1 cut(s) 237
BveI ACCTGC 1 cut(s) 902
Cac8I GCNNGC 4 cut(s) 115, 498, 637, 641
CaiI CAGNNNCTG 1 cut(s) 239
CciI TCATGA 1 cut(s) 225
CfoI GCGC 2 cut(s) 220, 826
Cfr10I RCCGGY 1 cut(s) 679
Cfr13I GGNCC 8 cut(s) 165, 193, 253, 277, 278, 401, 531, 578
Csp6I GTAC 4 cut(s) 64, 358, 379, 932
CviAII CATG 5 cut(s) 77, 221, 226, 457, 497
CviQI GTAC 4 cut(s) 64, 358, 379, 932
DdeI CTNAG 2 cut(s) 5, 908
DpnI GATC 5 cut(s) 10, 435, 464, 515, 927
DpnII GATC 5 cut(s) 8, 433, 462, 513, 925
DraI TTTAAA 1 cut(s) 901
DraIII CACNNNGTG 1 cut(s) 295
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
EciI GGCGGA 1 cut(s) 812
Eco130I CCWWGG 1 cut(s) 816
Eco24I GRGCYC 4 cut(s) 60, 180, 281, 564
Eco31I GGTCTC 1 cut(s) 91
Eco47I GGWCC 2 cut(s) 165, 531
Eco47III AGCGCT 1 cut(s) 825
Eco57I CTGAAG 1 cut(s) 288
Eco88I CYCGRG 2 cut(s) 179, 504
EcoRI GAATTC 1 cut(s) 200
EcoRII CCWGG 3 cut(s) 564, 613, 730
EcoT14I CCWWGG 1 cut(s) 816
EcoT38I GRGCYC 4 cut(s) 60, 180, 281, 564
ErhI CCWWGG 1 cut(s) 816
FaeI CATG 5 cut(s) 80, 224, 229, 460, 500
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FatI CATG 5 cut(s) 76, 220, 225, 456, 496
FbaI TGATCA 1 cut(s) 925
Fnu4HI GCNGC 7 cut(s) 39, 48, 189, 192, 501, 581, 822
FokI GGATG 3 cut(s) 191, 570, 906
FriOI GRGCYC 4 cut(s) 60, 180, 281, 564
Fsp4HI GCNGC 7 cut(s) 39, 48, 189, 192, 501, 581, 822
FspBI CTAG 3 cut(s) 51, 549, 872
GlaI GCGC 2 cut(s) 219, 825
GluI GCNGC 7 cut(s) 39, 48, 189, 192, 501, 581, 822
GsaI CCCAGC 2 cut(s) 278, 420
GsuI CTGGAG 1 cut(s) 63
HaeII RGCGCY 1 cut(s) 827
HaeIII GGCC 7 cut(s) 117, 194, 255, 279, 403, 580, 836
HapII CCGG 3 cut(s) 399, 486, 680
HhaI GCGC 2 cut(s) 220, 826
Hin1II CATG 5 cut(s) 80, 224, 229, 460, 500
Hin6I GCGC 2 cut(s) 218, 824
HinP1I GCGC 2 cut(s) 218, 824
HinfI GANTC 3 cut(s) 27, 317, 663
HpaII CCGG 3 cut(s) 399, 486, 680
HphI GGTGA 2 cut(s) 669, 883
Hpy166II GTNNAC 2 cut(s) 543, 606
Hpy188I TCNGA 2 cut(s) 8, 841
Hpy188III TCNNGA 4 cut(s) 181, 226, 437, 923
Hpy8I GTNNAC 2 cut(s) 543, 606
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 3 cut(s) 126, 134, 178
HpyCH4III ACNGT 3 cut(s) 349, 452, 540
HpyCH4IV ACGT 2 cut(s) 306, 468
HpyCH4V TGCA 4 cut(s) 233, 543, 672, 791
HpyF10VI GCNNNNNNNGC 7 cut(s) 44, 47, 549, 580, 645, 821, 894
HpyF3I CTNAG 2 cut(s) 5, 908
HpySE526I ACGT 2 cut(s) 306, 468
Hsp92II CATG 5 cut(s) 80, 224, 229, 460, 500
HspAI GCGC 2 cut(s) 218, 824
Ksp22I TGATCA 1 cut(s) 925
Kzo9I GATC 5 cut(s) 8, 433, 462, 513, 925
LguI GCTCTTC 1 cut(s) 264
LmnI GCTCC 4 cut(s) 44, 567, 688, 745
Lsp1109I GCAGC 5 cut(s) 50, 59, 200, 487, 833
LweI GCATC 1 cut(s) 592
MaeI CTAG 3 cut(s) 51, 549, 872
MaeII ACGT 2 cut(s) 306, 468
MaeIII GTNAC 2 cut(s) 318, 856
MalI GATC 5 cut(s) 10, 435, 464, 515, 927
MboI GATC 5 cut(s) 8, 433, 462, 513, 925
MboII GAAGA 1 cut(s) 281
MfeI CAATTG 1 cut(s) 667
MhlI GDGCHC 5 cut(s) 60, 180, 281, 545, 564
MluCI AATT 4 cut(s) 200, 480, 667, 687
MlyI GAGTC 1 cut(s) 326
MmeI TCCRAC 1 cut(s) 716
MseI TTAA 3 cut(s) 155, 783, 900
MspA1I CMGCKG 3 cut(s) 41, 113, 191
MspCI CTTAAG 1 cut(s) 782
MspI CCGG 3 cut(s) 399, 486, 680
MspR9I CCNGG 5 cut(s) 400, 487, 566, 615, 732
MunI CAATTG 1 cut(s) 667
Mva1269I GAATGC 1 cut(s) 305
MvaI CCWGG 3 cut(s) 566, 615, 732
MwoI GCNNNNNNNGC 7 cut(s) 44, 47, 549, 580, 645, 821, 894
NciI CCSGG 2 cut(s) 400, 487
NdeII GATC 5 cut(s) 8, 433, 462, 513, 925
NlaIII CATG 5 cut(s) 80, 224, 229, 460, 500
NlaIV GGNNCC 5 cut(s) 166, 279, 532, 563, 684
NmuCI GTSAC 1 cut(s) 318
NspI RCATGY 2 cut(s) 224, 500
NspV TTCGAA 1 cut(s) 661
PaeI GCATGC 1 cut(s) 500
PagI TCATGA 1 cut(s) 225
PaqCI CACCTGC 1 cut(s) 902
PciSI GCTCTTC 1 cut(s) 264
PcsI WCGNNNNNNNCGW 1 cut(s) 366
PctI GAATGC 1 cut(s) 305
PfeI GAWTC 2 cut(s) 27, 663
PflFI GACNNNGTC 1 cut(s) 452
PflMI CCANNNNNTGG 1 cut(s) 295
PkrI GCNGC 7 cut(s) 40, 49, 190, 193, 502, 582, 823
PleI GAGTC 1 cut(s) 325
PpsI GAGTC 1 cut(s) 325
Psp6I CCWGG 3 cut(s) 564, 613, 730
PspFI CCCAGC 2 cut(s) 274, 416
PspGI CCWGG 3 cut(s) 564, 613, 730
PspN4I GGNNCC 5 cut(s) 166, 279, 532, 563, 684
PspOMI GGGCCC 1 cut(s) 277
PspPI GGNCC 8 cut(s) 165, 193, 253, 277, 278, 401, 531, 578
PstNI CAGNNNCTG 1 cut(s) 239
PsyI GACNNNGTC 1 cut(s) 452
PvuII CAGCTG 1 cut(s) 41
RsaI GTAC 4 cut(s) 65, 359, 380, 933
RsaNI GTAC 4 cut(s) 64, 358, 379, 932
SapI GCTCTTC 1 cut(s) 264
SaqAI TTAA 3 cut(s) 155, 783, 900
SatI GCNGC 7 cut(s) 39, 48, 189, 192, 501, 581, 822
Sau3AI GATC 5 cut(s) 8, 433, 462, 513, 925
Sau96I GGNCC 8 cut(s) 165, 193, 253, 277, 278, 401, 531, 578
ScaI AGTACT 2 cut(s) 65, 933
SchI GAGTC 1 cut(s) 326
ScrFI CCNGG 5 cut(s) 400, 487, 566, 615, 732
SduI GDGCHC 5 cut(s) 60, 180, 281, 545, 564
SfaNI GCATC 1 cut(s) 592
SfcI CTRYAG 1 cut(s) 336
SfuI TTCGAA 1 cut(s) 661
SinI GGWCC 2 cut(s) 165, 531
SmlI CTYRAG 2 cut(s) 59, 782
SmoI CTYRAG 2 cut(s) 59, 782
SphI GCATGC 1 cut(s) 500
Sse9I AATT 4 cut(s) 200, 480, 667, 687
SsiI CCGC 6 cut(s) 111, 168, 191, 460, 581, 797
SspMI CTAG 3 cut(s) 51, 549, 872
StyD4I CCNGG 5 cut(s) 398, 485, 564, 613, 730
StyI CCWWGG 1 cut(s) 816
TaaI ACNGT 3 cut(s) 349, 452, 540
TaiI ACGT 2 cut(s) 309, 471
TaqI TCGA 6 cut(s) 369, 432, 438, 593, 661, 879
TasI AATT 4 cut(s) 200, 480, 667, 687
TatI WGTACW 3 cut(s) 63, 378, 931
TauI GCSGC 2 cut(s) 194, 583
TfiI GAWTC 2 cut(s) 27, 663
Tru1I TTAA 3 cut(s) 155, 783, 900
Tru9I TTAA 3 cut(s) 155, 783, 900
TscAI CASTG 1 cut(s) 244
TseFI GTSAC 1 cut(s) 318
TseI GCWGC 5 cut(s) 38, 47, 188, 500, 821
Tsp45I GTSAC 1 cut(s) 318
TspDTI ATGAA 2 cut(s) 193, 858
TspGWI ACGGA 2 cut(s) 375, 755
TspRI CASTG 1 cut(s) 244
Tth111I GACNNNGTC 1 cut(s) 452
Van91I CCANNNNNTGG 1 cut(s) 295
Vha464I CTTAAG 1 cut(s) 782
VneI GTGCAC 1 cut(s) 541
VpaK11BI GGWCC 2 cut(s) 165, 531
XapI RAATTY 1 cut(s) 200
XceI RCATGY 2 cut(s) 224, 500
XspI CTAG 3 cut(s) 51, 549, 872
ZrmI AGTACT 2 cut(s) 65, 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.