Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0008940

Physical Location & Seq

Genomic Coordinates Forward (+)

1806627 .. 1810370

Gene Structure 3744 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1029200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1455 bp

ATGCGGAGAGCGCGGCCGCAATTGAGCGCCATGTCGCGCGCTGCAATGATGTCGTTGTCGTCGTCAGCTTCGTCGTCTGCGGCCATGAGAAACCCTAGATTGTCCTTCTCTTCGTCCCCGAGGCTCTTTAACGGAGTGCCTCTCGGGAACCGCGTCTCGTTCTCCGCTGGCTTCCGCAGCACGTCGCTGCGGTGCTACGCTTCTTCCGCTGGCTTCGATAAAGTTCGGGTTCAGAATCCGATTGTCGAAATGGACGGTGATGAAATGGCGAGGATCATATGGACAACGATTAAAGACAAACTTATTTTTCCCTATCTGGACTTGGATATAAAGTATTATGATTTAGGGATTTTGAACCGTGATGCGACCGATGACAGAGTTACAGTAGAGAGTGCTGAAGCCACTCTTAAGTACAATGTTGCTGTGAAATGTGCTACAATAACCCCTGATGAGACCAGAGTGAAGGAGTTTGGGTTGAAATCAATGTGGAGGAGTCCAAACGGCACAATTAGAAACATTCTAGACGGTACTGTTTTCCGTGAACCTATCCTATGTAAAAACATCCCTAGAATAGTTCCTGGTTGGAAGAAACCCATCTGTATTGGTAGGCATGCCTTTGGTGATCAGTATCGAGCCACTGATACAGTTATCAAAGGGCCAGGAAAACTTAAAATGGTGTTTGTCCCGGAAGGAGGAGATGCCCCCGTTGAACTTGATGTTTACAATTTCAAAGGGCCTGGTGTGGCCCTTTCTATGTATAATGTTGATGAGTCTATTCGAGCTTTTGCTGACTCGTCAATGTCGTTGGCTCTTTCCAAGAAGTTGCCACTATACTTGAGCACCAAAAACACTATTCTTAAGAAATACGATGGCAGGTTTAAGGACATTTTTGAGGAGGTTTATGACGAGAAGTGGAAGCAGAAGTTTGAAGAAAATTCTATATGGTATGAACATCGGCTAATAGATGACATGGTGGCTTATGCACTCAAAAGTGAGGGTGGATATGTTTGGGCTTGCAAAAATTATGATGGAGATGTCATGAGTGATTTGCTTGCTCAAGGATTTGGCTCTTTGGGCCTCATGAGTTCTGTGTTGTTGTCTTCAGATGGCAAGACAATAGAAGCTGAAGCAGCTCATGGGACAGTAACTCGTCATTTTCGGTTACATCAGAAAGGACAAGAAACAAGTACAAATAGTATTGCTTCAATATTTGCATGGACACGAGGCCTAGAACACAGGGCAAAATTAGATAAAAATGATAGGTTGCTGGATTTTGTTCGCAAGTTGGAGGCTGCATGCATTGAGACAGTGGAGGGTGGGAAAATGACCAAGGATCTCGCCCTCTTGATCCATGGCCCCAAGGTATCGAGGGAGTACTACTTGAACACCGAAGAATTCATTGACGCTGTTGCCAAGAATGTTGAGGAAAAGCTTCTGGAACCTGCAGCGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0009507 GO:0009532 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0031976 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00020 ko00480 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 ko04146 map00020 map00480 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230 map04146

Pfam Domains

Protein Families

Iso_dh

Protein Analysis

485

Amino Acids

54.17

Weight (kDa)

8.64

Isoelectric Point (pI)

36.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Iso_dh	PF00180	77 - 470	3.6e-74	Isocitrate/isopropylmalate dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008940)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14590
fragaria_vesca	FvH4_6g51040 FvH4_6g51040 FvH4_6g51040
malus_domestica	MD09G1029200.v1.1 MD17G1031300.v1.1
prunus_persica	Prupe.3G288200_v2.0.a1 Prupe.3G288200_v2.0.a1 Prupe.3G288200_v2.0.a1
pyrus_communis	pycom111g02330 pycom17g02880
rosa_chinensis	RchiOBHm_Chr2g0172581 RchiOBHm_Chr3g0476461
rosa_laevigata	RLG00000022118
rosa_multiflora	Rmu_sc0000912.1_g000003 Rmu_ssc0000245.1_g000018
rosa_roxburghii	Rroxscaffold_2G00079740
rosa_rugosa	Rorug02G0560000
rosa_samantha	Rh2AG636200 Rh2BG649600 Rh2DG663900
rosa_wichuraiana	Rw2G052640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	864, 1450
AccII	CGCG	4 cut(s)	13, 37, 39, 153
AclWI	GGATC	3 cut(s)	281, 1341, 1342
AcoI	YGGCCR	2 cut(s)	14, 81
AcsI	RAATTY	2 cut(s)	934, 1394
AcuI	CTGAAG	3 cut(s)	417, 1086, 1146
AfaI	GTAC	4 cut(s)	413, 529, 1189, 1376
AfiI	CCNNNNNNNGG	1 cut(s)	692
AflII	CTTAAG	2 cut(s)	407, 857
AgsI	TTSAA	7 cut(s)	355, 478, 710, 730, 929, 1206, 1384
AjiI	CACGTC	1 cut(s)	183
AjnI	CCWGG	3 cut(s)	577, 658, 736
AluBI	AGCT	5 cut(s)	68, 782, 1124, 1133, 1432
AluI	AGCT	5 cut(s)	68, 782, 1124, 1133, 1432
Alw21I	GWGCWC	1 cut(s)	842
Alw26I	GTCTC	3 cut(s)	160, 446, 1298
AlwI	GGATC	3 cut(s)	281, 1341, 1342
Ama87I	CYCGRG	2 cut(s)	118, 143
AoxI	GGCC	8 cut(s)	14, 81, 656, 734, 744, 1075, 1225, 1354
ApeKI	GCWGC	6 cut(s)	41, 177, 187, 1130, 1292, 1445
ApoI	RAATTY	2 cut(s)	934, 1394
Asp700I	GAANNNNTTC	1 cut(s)	1431
AspLEI	GCGC	4 cut(s)	13, 29, 39, 41
AspS9I	GGNCC	5 cut(s)	656, 734, 745, 1075, 1355
AsuC2I	CCSGG	1 cut(s)	686
AsuHPI	GGTGA	2 cut(s)	269, 632
AvaI	CYCGRG	2 cut(s)	118, 143
BauI	CACGAG	1 cut(s)	1221
BbsI	GAAGAC	1 cut(s)	1092
Bbv12I	GWGCWC	1 cut(s)	842
BbvI	GCAGC	5 cut(s)	28, 174, 189, 1142, 1279
BccI	CCATC	4 cut(s)	602, 863, 1022, 1100
BceAI	ACGGC	1 cut(s)	517
BcgI	CGANNNNNNTGC	2 cut(s)	33, 67
BciT130I	CCWGG	3 cut(s)	579, 660, 738
BclI	TGATCA	1 cut(s)	622
BcnI	CCSGG	1 cut(s)	686
BcoDI	GTCTC	3 cut(s)	160, 446, 1298
BfaI	CTAG	4 cut(s)	96, 521, 567, 1229
BfmI	CTRYAG	1 cut(s)	1443
BfoI	RGCGCY	1 cut(s)	30
BfrI	CTTAAG	2 cut(s)	407, 857
BfuAI	ACCTGC	2 cut(s)	864, 1450
BisI	GCNGC	9 cut(s)	14, 17, 42, 81, 178, 188, 1131, 1293, 1446
BlsI	GCNGC	9 cut(s)	15, 18, 43, 82, 179, 189, 1132, 1294, 1447
BmcAI	AGTACT	1 cut(s)	1376
Bme1390I	CCNGG	4 cut(s)	579, 660, 686, 738
BmeT110I	CYCGRG	2 cut(s)	118, 143
BmgBI	CACGTC	1 cut(s)	183
BmgT120I	GGNCC	5 cut(s)	656, 734, 745, 1075, 1355
BmiI	GGNNCC	3 cut(s)	149, 1357, 1440
BmrFI	CCNGG	4 cut(s)	579, 660, 686, 738
BmsI	GCATC	2 cut(s)	352, 688
BpiI	GAAGAC	1 cut(s)	1092
BplI	GAGNNNNNCTC	2 cut(s)	126, 158
BpuEI	CTTGAG	2 cut(s)	856, 1041
BpuMI	CCSGG	1 cut(s)	686
BsaBI	GATNNNNATC	1 cut(s)	627
BsaI	GGTCTC	1 cut(s)	446
BsaJI	CCNNGG	4 cut(s)	119, 1329, 1351, 1359
BsaXI	ACNNNNNCTCC	2 cut(s)	458, 488
Bsc4I	CCNNNNNNNGG	1 cut(s)	692
Bse3DI	GCAATG	1 cut(s)	51
Bse8I	GATNNNNATC	1 cut(s)	627
BseBI	CCWGG	3 cut(s)	579, 660, 738
BseDI	CCNNGG	4 cut(s)	119, 1329, 1351, 1359
BseGI	GGATG	1 cut(s)	561
BseJI	GATNNNNATC	1 cut(s)	627
BseLI	CCNNNNNNNGG	1 cut(s)	692
BseMI	GCAATG	1 cut(s)	51
BsePI	GCGCGC	1 cut(s)	37
BseRI	GAGGAG	3 cut(s)	505, 708, 908
BseX3I	CGGCCG	1 cut(s)	14
BseXI	GCAGC	5 cut(s)	28, 174, 189, 1142, 1279
Bsh1236I	CGCG	4 cut(s)	13, 37, 39, 153
Bsh1285I	CGRYCG	2 cut(s)	17, 369
BshFI	GGCC	8 cut(s)	16, 83, 658, 736, 746, 1077, 1227, 1356
BsiEI	CGRYCG	2 cut(s)	17, 369
BsiHKAI	GWGCWC	1 cut(s)	842
BsiHKCI	CYCGRG	2 cut(s)	118, 143
BsiSI	CCGG	1 cut(s)	686
BslFI	GGGAC	3 cut(s)	100, 668, 1153
BslI	CCNNNNNNNGG	1 cut(s)	692
BsmAI	GTCTC	3 cut(s)	160, 446, 1298
BsmBI	CGTCTC	1 cut(s)	160
BsmFI	GGGAC	3 cut(s)	100, 668, 1153
BsnI	GGCC	8 cut(s)	16, 83, 658, 736, 746, 1077, 1227, 1356
Bso31I	GGTCTC	1 cut(s)	446
BsoBI	CYCGRG	2 cut(s)	118, 143
Bsp1286I	GDGCHC	1 cut(s)	842
Bsp143I	GATC	4 cut(s)	273, 622, 1333, 1347
Bsp19I	CCATGG	1 cut(s)	1351
BspANI	GGCC	8 cut(s)	16, 83, 658, 736, 746, 1077, 1227, 1356
BspFNI	CGCG	4 cut(s)	13, 37, 39, 153
BspHI	TCATGA	2 cut(s)	1038, 1080
BspLI	GGNNCC	3 cut(s)	149, 1357, 1440
BspMAI	CTGCAG	1 cut(s)	1447
BspMI	ACCTGC	2 cut(s)	864, 1450
BspPI	GGATC	3 cut(s)	281, 1341, 1342
BspTI	CTTAAG	2 cut(s)	407, 857
BspTNI	GGTCTC	1 cut(s)	446
BsrDI	GCAATG	1 cut(s)	51
BssECI	CCNNGG	4 cut(s)	119, 1329, 1351, 1359
BssHII	GCGCGC	1 cut(s)	37
BssMI	GATC	4 cut(s)	273, 622, 1333, 1347
BssSI	CACGAG	1 cut(s)	1221
BssT1I	CCWWGG	3 cut(s)	1329, 1351, 1359
Bst2BI	CACGAG	1 cut(s)	1221
Bst2UI	CCWGG	3 cut(s)	579, 660, 738
Bst4CI	ACNGT	8 cut(s)	257, 359, 385, 527, 532, 646, 1144, 1309
Bst6I	CTCTTC	1 cut(s)	115
BstAFI	CTTAAG	2 cut(s)	407, 857
BstC8I	GCNNGC	7 cut(s)	39, 169, 211, 612, 1015, 1053, 1297
BstDSI	CCRYGG	1 cut(s)	1351
BstF5I	GGATG	1 cut(s)	561
BstFNI	CGCG	4 cut(s)	13, 37, 39, 153
BstH2I	RGCGCY	1 cut(s)	30
BstHHI	GCGC	4 cut(s)	13, 29, 39, 41
BstKTI	GATC	4 cut(s)	276, 625, 1336, 1350
BstMAI	GTCTC	3 cut(s)	160, 446, 1298
BstMBI	GATC	4 cut(s)	273, 622, 1333, 1347
BstMCI	CGRYCG	2 cut(s)	17, 369
BstMWI	GCNNNNNNNGC	5 cut(s)	10, 177, 206, 1074, 1130
BstNI	CCWGG	3 cut(s)	579, 660, 738
BstNSI	RCATGY	2 cut(s)	614, 1299
BstSCI	CCNGG	4 cut(s)	577, 658, 684, 736
BstSFI	CTRYAG	1 cut(s)	1443
BstUI	CGCG	4 cut(s)	13, 37, 39, 153
BstV1I	GCAGC	5 cut(s)	28, 174, 189, 1142, 1279
BstV2I	GAAGAC	1 cut(s)	1092
BstX2I	RGATCY	1 cut(s)	1333
BstYI	RGATCY	1 cut(s)	1333
BstZI	CGGCCG	1 cut(s)	14
BsuRI	GGCC	8 cut(s)	16, 83, 658, 736, 746, 1077, 1227, 1356
BtgI	CCRYGG	1 cut(s)	1351
BtrI	CACGTC	1 cut(s)	183
BtsCI	GGATG	1 cut(s)	561
BtsIMutI	CAGTG	2 cut(s)	636, 1314
BveI	ACCTGC	2 cut(s)	864, 1450
Cac8I	GCNNGC	7 cut(s)	39, 169, 211, 612, 1015, 1053, 1297
CciI	TCATGA	2 cut(s)	1038, 1080
CciNI	GCGGCCGC	1 cut(s)	14
CfoI	GCGC	4 cut(s)	13, 29, 39, 41
Cfr13I	GGNCC	5 cut(s)	656, 734, 745, 1075, 1355
CseI	GACGC	2 cut(s)	142, 1412
Csp6I	GTAC	4 cut(s)	412, 528, 1188, 1375
CspCI	CAANNNNNGTGG	2 cut(s)	816, 851
CviQI	GTAC	4 cut(s)	412, 528, 1188, 1375
DpnI	GATC	4 cut(s)	275, 624, 1335, 1349
DpnII	GATC	4 cut(s)	273, 622, 1333, 1347
EaeI	YGGCCR	2 cut(s)	14, 81
EagI	CGGCCG	1 cut(s)	14
Eam1104I	CTCTTC	1 cut(s)	115
EarI	CTCTTC	1 cut(s)	115
EclXI	CGGCCG	1 cut(s)	14
Eco130I	CCWWGG	3 cut(s)	1329, 1351, 1359
Eco147I	AGGCCT	1 cut(s)	1227
Eco31I	GGTCTC	1 cut(s)	446
Eco52I	CGGCCG	1 cut(s)	14
Eco57I	CTGAAG	3 cut(s)	417, 1086, 1146
Eco88I	CYCGRG	2 cut(s)	118, 143
EcoO109I	RGGNCCY	1 cut(s)	734
EcoRI	GAATTC	1 cut(s)	1394
EcoRII	CCWGG	3 cut(s)	577, 658, 736
EcoT14I	CCWWGG	3 cut(s)	1329, 1351, 1359
EcoT22I	ATGCAT	1 cut(s)	1301
ErhI	CCWWGG	3 cut(s)	1329, 1351, 1359
Esp3I	CGTCTC	1 cut(s)	160
FalI	AAGNNNNNCTT	4 cut(s)	285, 317, 390, 422
FaqI	GGGAC	3 cut(s)	100, 668, 1153
FauNDI	CATATG	1 cut(s)	278
FbaI	TGATCA	1 cut(s)	622
Fnu4HI	GCNGC	9 cut(s)	14, 17, 42, 81, 178, 188, 1131, 1293, 1446
FokI	GGATG	1 cut(s)	548
Fsp4HI	GCNGC	9 cut(s)	14, 17, 42, 81, 178, 188, 1131, 1293, 1446
FspBI	CTAG	4 cut(s)	96, 521, 567, 1229
GlaI	GCGC	4 cut(s)	12, 28, 38, 40
GluI	GCNGC	9 cut(s)	14, 17, 42, 81, 178, 188, 1131, 1293, 1446
HaeII	RGCGCY	1 cut(s)	30
HaeIII	GGCC	8 cut(s)	16, 83, 658, 736, 746, 1077, 1227, 1356
HapII	CCGG	1 cut(s)	686
HgaI	GACGC	2 cut(s)	142, 1412
HhaI	GCGC	4 cut(s)	13, 29, 39, 41
Hin6I	GCGC	4 cut(s)	11, 27, 37, 39
HinP1I	GCGC	4 cut(s)	11, 27, 37, 39
HindIII	AAGCTT	1 cut(s)	1430
HinfI	GANTC	4 cut(s)	235, 493, 770, 791
HpaII	CCGG	1 cut(s)	686
HphI	GGTGA	2 cut(s)	269, 632
Hpy166II	GTNNAC	2 cut(s)	542, 721
Hpy188I	TCNGA	4 cut(s)	234, 240, 1105, 1170
Hpy188III	TCNNGA	7 cut(s)	145, 317, 521, 1039, 1081, 1345, 1436
Hpy8I	GTNNAC	2 cut(s)	542, 721
Hpy99I	CGWCG	3 cut(s)	64, 76, 187
HpyAV	CCTTC	3 cut(s)	115, 457, 683
HpyCH4III	ACNGT	8 cut(s)	257, 359, 385, 527, 532, 646, 1144, 1309
HpyCH4IV	ACGT	1 cut(s)	182
HpyCH4V	TGCA	7 cut(s)	44, 983, 1017, 1214, 1295, 1299, 1445
HpyF10VI	GCNNNNNNNGC	5 cut(s)	10, 177, 206, 1074, 1130
HpySE526I	ACGT	1 cut(s)	182
HspAI	GCGC	4 cut(s)	11, 27, 37, 39
Ksp22I	TGATCA	1 cut(s)	622
Kzo9I	GATC	4 cut(s)	273, 622, 1333, 1347
Lsp1109I	GCAGC	5 cut(s)	28, 174, 189, 1142, 1279
LweI	GCATC	2 cut(s)	352, 688
MaeI	CTAG	4 cut(s)	96, 521, 567, 1229
MaeII	ACGT	1 cut(s)	182
MaeIII	GTNAC	3 cut(s)	379, 1144, 1161
MalI	GATC	4 cut(s)	275, 624, 1335, 1349
MboI	GATC	4 cut(s)	273, 622, 1333, 1347
MboII	GAAGA	6 cut(s)	102, 195, 598, 941, 1092, 1403
MfeI	CAATTG	1 cut(s)	20
MflI	RGATCY	1 cut(s)	1333
MhlI	GDGCHC	1 cut(s)	842
MluCI	AATT	7 cut(s)	20, 507, 724, 934, 1021, 1244, 1394
MlyI	GAGTC	3 cut(s)	502, 779, 785
MmeI	TCCRAC	2 cut(s)	563, 1266
Mph1103I	ATGCAT	1 cut(s)	1301
MroXI	GAANNNNTTC	1 cut(s)	1431
MseI	TTAA	7 cut(s)	129, 291, 408, 669, 858, 879, 1453
MspA1I	CMGCKG	3 cut(s)	167, 209, 1448
MspCI	CTTAAG	2 cut(s)	407, 857
MspI	CCGG	1 cut(s)	686
MspR9I	CCNGG	4 cut(s)	579, 660, 686, 738
MunI	CAATTG	1 cut(s)	20
MvaI	CCWGG	3 cut(s)	579, 660, 738
MvnI	CGCG	4 cut(s)	13, 37, 39, 153
MwoI	GCNNNNNNNGC	5 cut(s)	10, 177, 206, 1074, 1130
NciI	CCSGG	1 cut(s)	686
NcoI	CCATGG	1 cut(s)	1351
NdeI	CATATG	1 cut(s)	278
NdeII	GATC	4 cut(s)	273, 622, 1333, 1347
NlaIV	GGNNCC	3 cut(s)	149, 1357, 1440
NotI	GCGGCCGC	1 cut(s)	14
NsiI	ATGCAT	1 cut(s)	1301
NspI	RCATGY	2 cut(s)	614, 1299
PaeI	GCATGC	2 cut(s)	614, 1299
PagI	TCATGA	2 cut(s)	1038, 1080
PauI	GCGCGC	1 cut(s)	37
PceI	AGGCCT	1 cut(s)	1227
PcsI	WCGNNNNNNNCGW	4 cut(s)	59, 68, 150, 800
PdmI	GAANNNNTTC	1 cut(s)	1431
PfeI	GAWTC	1 cut(s)	235
PfoI	TCCNGGA	1 cut(s)	684
PkrI	GCNGC	9 cut(s)	15, 18, 43, 82, 179, 189, 1132, 1294, 1447
PleI	GAGTC	3 cut(s)	501, 778, 785
PpsI	GAGTC	3 cut(s)	501, 778, 785
Psp6I	CCWGG	3 cut(s)	577, 658, 736
PspGI	CCWGG	3 cut(s)	577, 658, 736
PspN4I	GGNNCC	3 cut(s)	149, 1357, 1440
PspPI	GGNCC	5 cut(s)	656, 734, 745, 1075, 1355
PstI	CTGCAG	1 cut(s)	1447
PsuI	RGATCY	1 cut(s)	1333
PteI	GCGCGC	1 cut(s)	37
RsaI	GTAC	4 cut(s)	413, 529, 1189, 1376
RsaNI	GTAC	4 cut(s)	412, 528, 1188, 1375
SaqAI	TTAA	7 cut(s)	129, 291, 408, 669, 858, 879, 1453
SatI	GCNGC	9 cut(s)	14, 17, 42, 81, 178, 188, 1131, 1293, 1446
Sau3AI	GATC	4 cut(s)	273, 622, 1333, 1347
Sau96I	GGNCC	5 cut(s)	656, 734, 745, 1075, 1355
ScaI	AGTACT	1 cut(s)	1376
SchI	GAGTC	3 cut(s)	502, 779, 785
ScrFI	CCNGG	4 cut(s)	579, 660, 686, 738
SduI	GDGCHC	1 cut(s)	842
SfaNI	GCATC	2 cut(s)	352, 688
SfcI	CTRYAG	1 cut(s)	1443
SmlI	CTYRAG	4 cut(s)	407, 835, 857, 1056
SmoI	CTYRAG	4 cut(s)	407, 835, 857, 1056
SphI	GCATGC	2 cut(s)	614, 1299
Sse9I	AATT	7 cut(s)	20, 507, 724, 934, 1021, 1244, 1394
SseBI	AGGCCT	1 cut(s)	1227
SspI	AATATT	1 cut(s)	1209
SspMI	CTAG	4 cut(s)	96, 521, 567, 1229
StuI	AGGCCT	1 cut(s)	1227
StyD4I	CCNGG	4 cut(s)	577, 658, 684, 736
StyI	CCWWGG	3 cut(s)	1329, 1351, 1359
TaaI	ACNGT	8 cut(s)	257, 359, 385, 527, 532, 646, 1144, 1309
TaiI	ACGT	1 cut(s)	185
TaqI	TCGA	5 cut(s)	216, 246, 631, 778, 1367
TaqII	GACCGA	1 cut(s)	383
TasI	AATT	7 cut(s)	20, 507, 724, 934, 1021, 1244, 1394
TatI	WGTACW	3 cut(s)	411, 1187, 1374
TauI	GCSGC	3 cut(s)	16, 19, 83
TfiI	GAWTC	1 cut(s)	235
Tru1I	TTAA	7 cut(s)	129, 291, 408, 669, 858, 879, 1453
Tru9I	TTAA	7 cut(s)	129, 291, 408, 669, 858, 879, 1453
TscAI	CASTG	2 cut(s)	643, 1314
TseI	GCWGC	6 cut(s)	41, 177, 187, 1130, 1292, 1445
TspDTI	ATGAA	3 cut(s)	276, 963, 1387
TspGWI	ACGGA	2 cut(s)	147, 527
TspRI	CASTG	2 cut(s)	643, 1314
Vha464I	CTTAAG	2 cut(s)	407, 857
XapI	RAATTY	2 cut(s)	934, 1394
XbaI	TCTAGA	1 cut(s)	520
XceI	RCATGY	2 cut(s)	614, 1299
XmnI	GAANNNNTTC	1 cut(s)	1431
XspI	CTAG	4 cut(s)	96, 521, 567, 1229
ZrmI	AGTACT	1 cut(s)	1376
Zsp2I	ATGCAT	1 cut(s)	1301

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.