RchiOBHm_Chr3g0476461

Isocitrate dehydrogenase NADP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0008940
Physical Location & Seq
Forward (+)
22410078 .. 22411725
1648 bp
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UTR
Exon/CDS
Intron
PRQ44188

Sequence Viewer

Length: 312 bp
ATGATGAAGATGTCCAGAGTGATTTGCTTGCTCAAGAAGAGGAGTGGATTTGACTCTTTGGGCCTCATGACTTCTGTGCTGCTATCCTCAGATGGGAAAACAATAGAAGCTGAAGCAGCTCATGGGACAGTAACTCGTCATTTTCGGTTGCATCAGAAGGGACAAGAAACCAGTACAAACAGTATTGCTTCAATATTTGCATGGACAAGAGGCCTAGAGCATAGGGGCAAATTAGATAAAAATGACAGGTTGCTAGATTTTGTTCGAAAGTTGGAGGCTGCATGCATTGAGACAGTGGAGGCGGAAACATGA

Protein Analysis

103

Amino Acids

11.52

Weight (kDa)

9.1

Isoelectric Point (pI)

32.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Iso_dh PF00180 17 - 102 7e-13 Isocitrate/isopropylmalate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 302
AcuI CTGAAG 1 cut(s) 132
AfaI GTAC 1 cut(s) 175
AfiI CCNNNNNNNGG 1 cut(s) 93
AgsI TTSAA 1 cut(s) 192
AluBI AGCT 2 cut(s) 110, 119
AluI AGCT 2 cut(s) 110, 119
Alw26I GTCTC 1 cut(s) 284
AoxI GGCC 2 cut(s) 61, 211
ApeKI GCWGC 3 cut(s) 79, 116, 278
AspS9I GGNCC 1 cut(s) 61
AsuII TTCGAA 1 cut(s) 265
BbvI GCAGC 3 cut(s) 66, 128, 265
BccI CCATC 1 cut(s) 86
BcoDI GTCTC 1 cut(s) 284
BfaI CTAG 2 cut(s) 215, 254
BisI GCNGC 3 cut(s) 80, 117, 279
BlsI GCNGC 3 cut(s) 81, 118, 280
BmgT120I GGNCC 1 cut(s) 61
BmsI GCATC 1 cut(s) 160
Bpu14I TTCGAA 1 cut(s) 265
BpuEI CTTGAG 1 cut(s) 17
Bsc4I CCNNNNNNNGG 1 cut(s) 93
Bse1I ACTGG 1 cut(s) 171
BseLI CCNNNNNNNGG 1 cut(s) 93
BseMII CTCAG 1 cut(s) 102
BseNI ACTGG 1 cut(s) 171
BseRI GAGGAG 1 cut(s) 55
BseXI GCAGC 3 cut(s) 66, 128, 265
BshFI GGCC 2 cut(s) 63, 213
BslFI GGGAC 2 cut(s) 139, 174
BslI CCNNNNNNNGG 1 cut(s) 93
BsmAI GTCTC 1 cut(s) 284
BsmFI GGGAC 2 cut(s) 139, 174
BsnI GGCC 2 cut(s) 63, 213
Bsp119I TTCGAA 1 cut(s) 265
BspACI CCGC 1 cut(s) 302
BspANI GGCC 2 cut(s) 63, 213
BspCNI CTCAG 1 cut(s) 101
BspHI TCATGA 1 cut(s) 66
BspT104I TTCGAA 1 cut(s) 265
BsrI ACTGG 1 cut(s) 171
Bst4CI ACNGT 3 cut(s) 130, 182, 295
Bst6I CTCTTC 1 cut(s) 32
BstBI TTCGAA 1 cut(s) 265
BstC8I GCNNGC 2 cut(s) 29, 283
BstDEI CTNAG 1 cut(s) 88
BstMAI GTCTC 1 cut(s) 284
BstMWI GCNNNNNNNGC 1 cut(s) 116
BstNSI RCATGY 1 cut(s) 285
BstV1I GCAGC 3 cut(s) 66, 128, 265
BsuRI GGCC 2 cut(s) 63, 213
BtsIMutI CAGTG 1 cut(s) 300
Cac8I GCNNGC 2 cut(s) 29, 283
CciI TCATGA 1 cut(s) 66
Cfr13I GGNCC 1 cut(s) 61
Csp6I GTAC 1 cut(s) 174
CviAII CATG 5 cut(s) 67, 122, 201, 282, 309
CviJI RGCY 5 cut(s) 63, 110, 119, 213, 278
CviKI_1 RGCY 5 cut(s) 63, 110, 119, 213, 278
CviQI GTAC 1 cut(s) 174
DdeI CTNAG 1 cut(s) 88
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Eco147I AGGCCT 1 cut(s) 213
Eco57I CTGAAG 1 cut(s) 132
EcoT22I ATGCAT 1 cut(s) 287
FaeI CATG 5 cut(s) 70, 125, 204, 285, 312
FaiI YATR 6 cut(s) 68, 123, 202, 222, 283, 310
FaqI GGGAC 2 cut(s) 139, 174
FatI CATG 5 cut(s) 66, 121, 200, 281, 308
Fnu4HI GCNGC 3 cut(s) 80, 117, 279
Fsp4HI GCNGC 3 cut(s) 80, 117, 279
FspBI CTAG 2 cut(s) 215, 254
GluI GCNGC 3 cut(s) 80, 117, 279
HaeIII GGCC 2 cut(s) 63, 213
Hin1II CATG 5 cut(s) 70, 125, 204, 285, 312
HinfI GANTC 1 cut(s) 53
Hpy188I TCNGA 2 cut(s) 91, 156
Hpy188III TCNNGA 3 cut(s) 15, 34, 67
HpyAV CCTTC 1 cut(s) 151
HpyCH4III ACNGT 3 cut(s) 130, 182, 295
HpyCH4V TGCA 4 cut(s) 151, 200, 281, 285
HpyF10VI GCNNNNNNNGC 1 cut(s) 116
HpyF3I CTNAG 1 cut(s) 88
Hsp92II CATG 5 cut(s) 70, 125, 204, 285, 312
LpnPI CCDG 3 cut(s) 28, 184, 232
Lsp1109I GCAGC 3 cut(s) 66, 128, 265
LweI GCATC 1 cut(s) 160
MaeI CTAG 2 cut(s) 215, 254
MaeIII GTNAC 1 cut(s) 130
MboII GAAGA 2 cut(s) 19, 49
MluCI AATT 1 cut(s) 230
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 1 cut(s) 252
MnlI CCTC 6 cut(s) 33, 74, 97, 203, 268, 292
Mph1103I ATGCAT 1 cut(s) 287
MwoI GCNNNNNNNGC 1 cut(s) 116
NlaIII CATG 5 cut(s) 70, 125, 204, 285, 312
NsiI ATGCAT 1 cut(s) 287
NspI RCATGY 1 cut(s) 285
NspV TTCGAA 1 cut(s) 265
PaeI GCATGC 1 cut(s) 285
PagI TCATGA 1 cut(s) 66
PceI AGGCCT 1 cut(s) 213
PkrI GCNGC 3 cut(s) 81, 118, 280
PleI GAGTC 1 cut(s) 47
PpsI GAGTC 1 cut(s) 47
PspPI GGNCC 1 cut(s) 61
RsaI GTAC 1 cut(s) 175
RsaNI GTAC 1 cut(s) 174
SatI GCNGC 3 cut(s) 80, 117, 279
Sau96I GGNCC 1 cut(s) 61
SchI GAGTC 1 cut(s) 47
SetI ASST 3 cut(s) 112, 121, 251
SfaNI GCATC 1 cut(s) 160
SfuI TTCGAA 1 cut(s) 265
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
SphI GCATGC 1 cut(s) 285
Sse9I AATT 1 cut(s) 230
SseBI AGGCCT 1 cut(s) 213
SsiI CCGC 1 cut(s) 302
SspI AATATT 1 cut(s) 195
SspMI CTAG 2 cut(s) 215, 254
StuI AGGCCT 1 cut(s) 213
TaaI ACNGT 3 cut(s) 130, 182, 295
TaqI TCGA 1 cut(s) 265
TasI AATT 1 cut(s) 230
TatI WGTACW 1 cut(s) 173
TscAI CASTG 1 cut(s) 300
TseI GCWGC 3 cut(s) 79, 116, 278
TspDTI ATGAA 1 cut(s) 20
TspRI CASTG 1 cut(s) 300
XceI RCATGY 1 cut(s) 285
XspI CTAG 2 cut(s) 215, 254
Zsp2I ATGCAT 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.