RLG00000022118

Isocitrate dehydrogenase NADP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008940
Physical Location & Seq
Forward (+)
81052510 .. 81056720
4211 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022118

Sequence Viewer

Length: 1317 bp
ATGTCGCGCGCCTCCATGATATCTTCTTCTTCGTCTTCTATAGCCCCGAGAAACCCTAGCTTCTCCTTCACTTCCACGCCGAGGCGCTTCAATGGAGTCGGCAACCGCGTCTCTTTCGCCGCAACCTCTAATCGCACTTCGCTCCGGTGCTACGCTGCTACTTCCGGTGTCTTCGAGAAAGTCCGGGTTCAGAATCCGATCGTCGAAATGGACGGTGATGAAATGACGAGGATCATATGGACAGCAATCAAAGAAAAACTTATTTTCCCCTATGTGGATTTGGACATAAAGTATTATGATTTAGGGATTGAGAACCGTGATGCTACTGATGACAAAGTTACGGTAGAGAGTGCTGAAGCCACTCTTAAGTTCATTCTTTATTTTGTAGGTACTGTCTTTCGTGAACCTATCCTATGCCGAAACATTCCTAGAATAGTTCCTGGCTGGAAGAAACCCATCTGTATTGGTAGGCATGCTTTCGGCGATCAGTATCGAGCTACTGATACAGTTATCACGGGACCAGGAAAGCTGAAAATGGTATTTGTCCCAGAAGGAGGAGACACCCCGACGGAGCTTAATGTCTATGATTTCAAAGGTCCTGGTGTGGCCCTCGCTATGTATAATGTTGATGAGTCTATTCGAGCTTTTGCTGACTCTTCCATGTCGATGGCATTTTCCAAGAAGTGGCCACTATACTTGAGCACCAAAAACACAATTCTTAAGAAATATGATGGCAGGTTTAAGGACATATTCGAGGAGGTTTATGTGGAAAAGTGGAAGCAGAAGTTTGAAGAAAACTCTATATGGTATGAGCATCGGCTAATAGATGACATGGTGGCTTATGCACTCAAAAGTGAGGGTGGATATGTTTGGGCTTGCAAAAACTATGATGGAGATGTCCAGAGTGATTTGCTTGCTCAAGGATTTGGCTCTTTGGGTCTCATGACTTCTGTGCTGCTATCCTCAGATGGGAAAACAATAGAAGCTGAAGCAGCTCATGGGACAGTAACTCGTCATTTTCGGTTACATCAGAAGGGACAAGAAACCAGTACAAACAGTATTGCTTCAATATTTGCATGGACAAGAGGCCTAGAGCATAGGGGCAAATTAGATAAAAATGACAGGTTGCTAGATTTTGTTCGAAAGTTGGAGGCTGCATGCATTGAGACAGTGGAGGCAGGAAACATGACTAAGGATCTCGCCTTATTGATCCATGGCCCCAAGGTGTCAAGGGAGTTCTATTTGAACACAGAAGAGTTTATTGACACAGTGGCAAAGAATGTTGAGCAAAAGCTTCGGGAACCTGCTCTGGTCTAA

Protein Analysis

439

Amino Acids

49.37

Weight (kDa)

7.06

Isoelectric Point (pI)

29.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Iso_dh PF00180 133 - 424 4.2e-52 Isocitrate/isopropylmalate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 726, 1312
AccB7I CCANNNNNTGG 1 cut(s) 684
AccII CGCG 3 cut(s) 7, 9, 108
AciI CCGC 2 cut(s) 106, 120
AclWI GGATC 3 cut(s) 239, 1203, 1204
AcoI YGGCCR 1 cut(s) 686
AcuI CTGAAG 2 cut(s) 375, 1008
AfaI GTAC 2 cut(s) 391, 1051
AfiI CCNNNNNNNGG 5 cut(s) 81, 274, 554, 684, 969
AflII CTTAAG 2 cut(s) 365, 719
AgsI TTSAA 5 cut(s) 91, 592, 791, 1068, 1246
AjnI CCWGG 3 cut(s) 439, 520, 598
AluBI AGCT 8 cut(s) 60, 497, 529, 574, 644, 986, 995, 1294
AluI AGCT 8 cut(s) 60, 497, 529, 574, 644, 986, 995, 1294
Alw21I GWGCWC 1 cut(s) 704
Alw26I GTCTC 4 cut(s) 115, 552, 944, 1160
AlwI GGATC 3 cut(s) 239, 1203, 1204
Ama87I CYCGRG 1 cut(s) 46
AoxI GGCC 4 cut(s) 606, 686, 1087, 1216
ApeKI GCWGC 4 cut(s) 155, 955, 992, 1154
AspLEI GCGC 3 cut(s) 9, 11, 87
AspS9I GGNCC 4 cut(s) 518, 596, 607, 1217
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 1 cut(s) 227
AsuII TTCGAA 1 cut(s) 1141
AvaI CYCGRG 1 cut(s) 46
AvaII GGWCC 2 cut(s) 518, 596
BalI TGGCCA 1 cut(s) 688
BbsI GAAGAC 2 cut(s) 27, 163
Bbv12I GWGCWC 1 cut(s) 704
BbvI GCAGC 4 cut(s) 142, 942, 1004, 1141
BccI CCATC 5 cut(s) 464, 661, 725, 884, 962
BcgI CGANNNNNNTGC 2 cut(s) 1277, 1311
BciT130I CCWGG 3 cut(s) 441, 522, 600
BcnI CCSGG 1 cut(s) 185
BcoDI GTCTC 4 cut(s) 115, 552, 944, 1160
BfaI CTAG 4 cut(s) 57, 429, 1091, 1130
BfmI CTRYAG 1 cut(s) 39
BfoI RGCGCY 1 cut(s) 88
BfrI CTTAAG 2 cut(s) 365, 719
BfuAI ACCTGC 2 cut(s) 726, 1312
BisI GCNGC 5 cut(s) 120, 156, 956, 993, 1155
BlsI GCNGC 5 cut(s) 121, 157, 957, 994, 1156
Bme1390I CCNGG 4 cut(s) 185, 441, 522, 600
Bme18I GGWCC 2 cut(s) 518, 596
BmeT110I CYCGRG 1 cut(s) 46
BmgT120I GGNCC 4 cut(s) 518, 596, 607, 1217
BmiI GGNNCC 3 cut(s) 519, 1219, 1302
BmrFI CCNGG 4 cut(s) 185, 441, 522, 600
BmsI GCATC 2 cut(s) 310, 823
BpiI GAAGAC 2 cut(s) 27, 163
Bpu14I TTCGAA 1 cut(s) 1141
BpuEI CTTGAG 2 cut(s) 718, 903
BpuMI CCSGG 1 cut(s) 185
BsaBI GATNNNNATC 1 cut(s) 489
BsaI GGTCTC 1 cut(s) 944
BsaJI CCNNGG 3 cut(s) 80, 1213, 1221
BsaWI WCCGGW 2 cut(s) 144, 164
Bsc4I CCNNNNNNNGG 5 cut(s) 81, 274, 554, 684, 969
Bse1I ACTGG 1 cut(s) 1047
Bse8I GATNNNNATC 1 cut(s) 489
BseBI CCWGG 3 cut(s) 441, 522, 600
BseDI CCNNGG 3 cut(s) 80, 1213, 1221
BseJI GATNNNNATC 1 cut(s) 489
BseLI CCNNNNNNNGG 5 cut(s) 81, 274, 554, 684, 969
BseMII CTCAG 1 cut(s) 978
BseNI ACTGG 1 cut(s) 1047
BsePI GCGCGC 1 cut(s) 7
BseRI GAGGAG 2 cut(s) 570, 770
BseXI GCAGC 4 cut(s) 142, 942, 1004, 1141
Bsh1236I CGCG 3 cut(s) 7, 9, 108
Bsh1285I CGRYCG 1 cut(s) 201
BshFI GGCC 4 cut(s) 608, 688, 1089, 1218
BsiEI CGRYCG 1 cut(s) 201
BsiHKAI GWGCWC 1 cut(s) 704
BsiHKCI CYCGRG 1 cut(s) 46
BsiSI CCGG 3 cut(s) 145, 165, 184
BslFI GGGAC 4 cut(s) 530, 531, 1015, 1050
BslI CCNNNNNNNGG 5 cut(s) 81, 274, 554, 684, 969
BsmAI GTCTC 4 cut(s) 115, 552, 944, 1160
BsmBI CGTCTC 1 cut(s) 115
BsmFI GGGAC 4 cut(s) 530, 531, 1015, 1050
BsnI GGCC 4 cut(s) 608, 688, 1089, 1218
Bso31I GGTCTC 1 cut(s) 944
BsoBI CYCGRG 1 cut(s) 46
Bsp119I TTCGAA 1 cut(s) 1141
Bsp1286I GDGCHC 1 cut(s) 704
Bsp143I GATC 5 cut(s) 198, 231, 484, 1195, 1209
Bsp19I CCATGG 1 cut(s) 1213
BspACI CCGC 2 cut(s) 106, 120
BspANI GGCC 4 cut(s) 608, 688, 1089, 1218
BspCNI CTCAG 1 cut(s) 977
BspFNI CGCG 3 cut(s) 7, 9, 108
BspHI TCATGA 1 cut(s) 942
BspLI GGNNCC 3 cut(s) 519, 1219, 1302
BspMI ACCTGC 2 cut(s) 726, 1312
BspPI GGATC 3 cut(s) 239, 1203, 1204
BspT104I TTCGAA 1 cut(s) 1141
BspTI CTTAAG 2 cut(s) 365, 719
BspTNI GGTCTC 1 cut(s) 944
BsrI ACTGG 1 cut(s) 1047
BssECI CCNNGG 3 cut(s) 80, 1213, 1221
BssHII GCGCGC 1 cut(s) 7
BssMI GATC 5 cut(s) 198, 231, 484, 1195, 1209
BssT1I CCWWGG 2 cut(s) 1213, 1221
Bst2UI CCWGG 3 cut(s) 441, 522, 600
Bst4CI ACNGT 9 cut(s) 215, 317, 343, 394, 508, 1006, 1058, 1171, 1270
Bst6I CTCTTC 2 cut(s) 661, 1248
BstAFI CTTAAG 2 cut(s) 365, 719
BstBI TTCGAA 1 cut(s) 1141
BstC8I GCNNGC 5 cut(s) 9, 474, 877, 915, 1159
BstDEI CTNAG 2 cut(s) 964, 1191
BstDSI CCRYGG 1 cut(s) 1213
BstFNI CGCG 3 cut(s) 7, 9, 108
BstH2I RGCGCY 1 cut(s) 88
BstHHI GCGC 3 cut(s) 9, 11, 87
BstKTI GATC 5 cut(s) 201, 234, 487, 1198, 1212
BstMAI GTCTC 4 cut(s) 115, 552, 944, 1160
BstMBI GATC 5 cut(s) 198, 231, 484, 1195, 1209
BstMCI CGRYCG 1 cut(s) 201
BstMWI GCNNNNNNNGC 1 cut(s) 992
BstNI CCWGG 3 cut(s) 441, 522, 600
BstNSI RCATGY 2 cut(s) 476, 1161
BstSCI CCNGG 4 cut(s) 183, 439, 520, 598
BstSFI CTRYAG 1 cut(s) 39
BstUI CGCG 3 cut(s) 7, 9, 108
BstV1I GCAGC 4 cut(s) 142, 942, 1004, 1141
BstV2I GAAGAC 2 cut(s) 27, 163
BstX2I RGATCY 1 cut(s) 1195
BstXI CCANNNNNNTGG 1 cut(s) 667
BstYI RGATCY 1 cut(s) 1195
BsuRI GGCC 4 cut(s) 608, 688, 1089, 1218
BtgI CCRYGG 1 cut(s) 1213
BtsIMutI CAGTG 2 cut(s) 1176, 1275
BveI ACCTGC 2 cut(s) 726, 1312
Cac8I GCNNGC 5 cut(s) 9, 474, 877, 915, 1159
CciI TCATGA 1 cut(s) 942
CfoI GCGC 3 cut(s) 9, 11, 87
Cfr13I GGNCC 4 cut(s) 518, 596, 607, 1217
CseI GACGC 1 cut(s) 97
Csp6I GTAC 2 cut(s) 390, 1050
CspCI CAANNNNNGTGG 2 cut(s) 678, 713
CviQI GTAC 2 cut(s) 390, 1050
DdeI CTNAG 2 cut(s) 964, 1191
DpnI GATC 5 cut(s) 200, 233, 486, 1197, 1211
DpnII GATC 5 cut(s) 198, 231, 484, 1195, 1209
EaeI YGGCCR 1 cut(s) 686
Eam1104I CTCTTC 2 cut(s) 661, 1248
EarI CTCTTC 2 cut(s) 661, 1248
Eco130I CCWWGG 2 cut(s) 1213, 1221
Eco147I AGGCCT 1 cut(s) 1089
Eco31I GGTCTC 1 cut(s) 944
Eco32I GATATC 1 cut(s) 21
Eco47I GGWCC 2 cut(s) 518, 596
Eco57I CTGAAG 2 cut(s) 375, 1008
Eco88I CYCGRG 1 cut(s) 46
EcoO109I RGGNCCY 1 cut(s) 596
EcoRII CCWGG 3 cut(s) 439, 520, 598
EcoRV GATATC 1 cut(s) 21
EcoT14I CCWWGG 2 cut(s) 1213, 1221
EcoT22I ATGCAT 1 cut(s) 1163
ErhI CCWWGG 2 cut(s) 1213, 1221
Esp3I CGTCTC 1 cut(s) 115
FalI AAGNNNNNCTT 4 cut(s) 243, 275, 348, 380
FaqI GGGAC 4 cut(s) 530, 531, 1015, 1050
FauNDI CATATG 1 cut(s) 236
Fnu4HI GCNGC 5 cut(s) 120, 156, 956, 993, 1155
Fsp4HI GCNGC 5 cut(s) 120, 156, 956, 993, 1155
FspBI CTAG 4 cut(s) 57, 429, 1091, 1130
GlaI GCGC 3 cut(s) 8, 10, 86
GluI GCNGC 5 cut(s) 120, 156, 956, 993, 1155
HaeII RGCGCY 1 cut(s) 88
HaeIII GGCC 4 cut(s) 608, 688, 1089, 1218
HapII CCGG 3 cut(s) 145, 165, 184
HgaI GACGC 1 cut(s) 97
HhaI GCGC 3 cut(s) 9, 11, 87
Hin6I GCGC 3 cut(s) 7, 9, 85
HinP1I GCGC 3 cut(s) 7, 9, 85
HindIII AAGCTT 1 cut(s) 1292
HinfI GANTC 4 cut(s) 96, 193, 632, 653
HpaII CCGG 3 cut(s) 145, 165, 184
HphI GGTGA 1 cut(s) 227
Hpy166II GTNNAC 1 cut(s) 404
Hpy188I TCNGA 4 cut(s) 192, 198, 967, 1032
Hpy188III TCNNGA 5 cut(s) 175, 401, 901, 943, 1298
Hpy8I GTNNAC 1 cut(s) 404
Hpy99I CGWCG 2 cut(s) 206, 571
HpyAV CCTTC 3 cut(s) 76, 545, 1027
HpyCH4III ACNGT 9 cut(s) 215, 317, 343, 394, 508, 1006, 1058, 1171, 1270
HpyCH4V TGCA 5 cut(s) 845, 879, 1076, 1157, 1161
HpyF10VI GCNNNNNNNGC 1 cut(s) 992
HpyF3I CTNAG 2 cut(s) 964, 1191
HspAI GCGC 3 cut(s) 7, 9, 85
Kzo9I GATC 5 cut(s) 198, 231, 484, 1195, 1209
LmnI GCTCC 2 cut(s) 147, 571
Lsp1109I GCAGC 4 cut(s) 142, 942, 1004, 1141
LweI GCATC 2 cut(s) 310, 823
MaeI CTAG 4 cut(s) 57, 429, 1091, 1130
MaeIII GTNAC 3 cut(s) 337, 1006, 1023
MalI GATC 5 cut(s) 200, 233, 486, 1197, 1211
MboI GATC 5 cut(s) 198, 231, 484, 1195, 1209
MboII GAAGA 9 cut(s) 15, 18, 21, 27, 163, 460, 648, 803, 1265
MflI RGATCY 1 cut(s) 1195
MhlI GDGCHC 1 cut(s) 704
MlsI TGGCCA 1 cut(s) 688
MluCI AATT 2 cut(s) 714, 1106
MluNI TGGCCA 1 cut(s) 688
MlyI GAGTC 3 cut(s) 105, 641, 647
MmeI TCCRAC 1 cut(s) 1128
Mox20I TGGCCA 1 cut(s) 688
Mph1103I ATGCAT 1 cut(s) 1163
MscI TGGCCA 1 cut(s) 688
MseI TTAA 4 cut(s) 366, 576, 720, 741
MslI CAYNNNNRTG 1 cut(s) 665
Msp20I TGGCCA 1 cut(s) 688
MspCI CTTAAG 2 cut(s) 365, 719
MspI CCGG 3 cut(s) 145, 165, 184
MspR9I CCNGG 4 cut(s) 185, 441, 522, 600
MvaI CCWGG 3 cut(s) 441, 522, 600
MvnI CGCG 3 cut(s) 7, 9, 108
MwoI GCNNNNNNNGC 1 cut(s) 992
NciI CCSGG 1 cut(s) 185
NcoI CCATGG 1 cut(s) 1213
NdeI CATATG 1 cut(s) 236
NdeII GATC 5 cut(s) 198, 231, 484, 1195, 1209
NlaIV GGNNCC 3 cut(s) 519, 1219, 1302
NmeAIII GCCGAG 1 cut(s) 105
NsiI ATGCAT 1 cut(s) 1163
NspI RCATGY 2 cut(s) 476, 1161
NspV TTCGAA 1 cut(s) 1141
PaeI GCATGC 2 cut(s) 476, 1161
PagI TCATGA 1 cut(s) 942
PauI GCGCGC 1 cut(s) 7
PceI AGGCCT 1 cut(s) 1089
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PfeI GAWTC 1 cut(s) 193
PflMI CCANNNNNTGG 1 cut(s) 684
PkrI GCNGC 5 cut(s) 121, 157, 957, 994, 1156
Ple19I CGATCG 1 cut(s) 201
PleI GAGTC 3 cut(s) 104, 640, 647
PpsI GAGTC 3 cut(s) 104, 640, 647
PpuMI RGGWCCY 1 cut(s) 596
Psp5II RGGWCCY 1 cut(s) 596
Psp6I CCWGG 3 cut(s) 439, 520, 598
PspGI CCWGG 3 cut(s) 439, 520, 598
PspN4I GGNNCC 3 cut(s) 519, 1219, 1302
PspPI GGNCC 4 cut(s) 518, 596, 607, 1217
PspPPI RGGWCCY 1 cut(s) 596
PsuI RGATCY 1 cut(s) 1195
PteI GCGCGC 1 cut(s) 7
PvuI CGATCG 1 cut(s) 201
RsaI GTAC 2 cut(s) 391, 1051
RsaNI GTAC 2 cut(s) 390, 1050
RseI CAYNNNNRTG 1 cut(s) 665
SaqAI TTAA 4 cut(s) 366, 576, 720, 741
SatI GCNGC 5 cut(s) 120, 156, 956, 993, 1155
Sau3AI GATC 5 cut(s) 198, 231, 484, 1195, 1209
Sau96I GGNCC 4 cut(s) 518, 596, 607, 1217
SchI GAGTC 3 cut(s) 105, 641, 647
ScrFI CCNGG 4 cut(s) 185, 441, 522, 600
SduI GDGCHC 1 cut(s) 704
SfaNI GCATC 2 cut(s) 310, 823
SfcI CTRYAG 1 cut(s) 39
SfuI TTCGAA 1 cut(s) 1141
SinI GGWCC 2 cut(s) 518, 596
SmiMI CAYNNNNRTG 1 cut(s) 665
SmlI CTYRAG 4 cut(s) 365, 697, 719, 918
SmoI CTYRAG 4 cut(s) 365, 697, 719, 918
SphI GCATGC 2 cut(s) 476, 1161
Sse9I AATT 2 cut(s) 714, 1106
SseBI AGGCCT 1 cut(s) 1089
SsiI CCGC 2 cut(s) 106, 120
SspI AATATT 1 cut(s) 1071
SspMI CTAG 4 cut(s) 57, 429, 1091, 1130
StuI AGGCCT 1 cut(s) 1089
StyD4I CCNGG 4 cut(s) 183, 439, 520, 598
StyI CCWWGG 2 cut(s) 1213, 1221
TaaI ACNGT 9 cut(s) 215, 317, 343, 394, 508, 1006, 1058, 1171, 1270
TaqI TCGA 7 cut(s) 174, 204, 493, 640, 665, 753, 1141
TasI AATT 2 cut(s) 714, 1106
TatI WGTACW 1 cut(s) 1049
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 1 cut(s) 193
Tru1I TTAA 4 cut(s) 366, 576, 720, 741
Tru9I TTAA 4 cut(s) 366, 576, 720, 741
TscAI CASTG 2 cut(s) 1176, 1275
TseI GCWGC 4 cut(s) 155, 955, 992, 1154
TspDTI ATGAA 2 cut(s) 234, 361
TspGWI ACGGA 1 cut(s) 584
TspRI CASTG 2 cut(s) 1176, 1275
Van91I CCANNNNNTGG 1 cut(s) 684
Vha464I CTTAAG 2 cut(s) 365, 719
VpaK11BI GGWCC 2 cut(s) 518, 596
XceI RCATGY 2 cut(s) 476, 1161
XspI CTAG 4 cut(s) 57, 429, 1091, 1130
Zsp2I ATGCAT 1 cut(s) 1163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.