Rh2AG636200

Isocitrate dehydrogenase NADP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0008940
Physical Location & Seq
Forward (+)
86115607 .. 86119831
4225 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG636200.1

Sequence Viewer

Length: 1443 bp
ATGCTAAAGGCGAGACTCCAATTTAGCGCCATGTCGCGCGCCGCCATGATATCTTCTTCTTCGTCTTCTATAGCCCCGAGAAACCCTAGCTTCTCCTTCACTTCCACGCCGAGGCGCTTCAATGGAGTCGGCAACCGCGTCTCTTTCGCCGCAACCTCTAATCGCACTTCGCTCCGGTGCTACGCTGCTACTTCCGATGGCTTCGAGAAAGTCCGGGTTCAGAATCCGATCGTCGAAATGGACGGTGATGAAATGACGAGGATCATATGGACAGCAATTAAAGAAAAACTTATTTTCCCCTATGTGGATTTGGACATAAAGTATTATGATTTAGGGATTGAGAACCGTGATGCTACTGATGACAAAGTTACGGTAGAGAGTGCCGAAGCCACTCTTAAGTACAATGTTGCTGTGAAATGTGCTACAATAACTCCTGATGAGACCAGAATGAAGGAGTTTGGGCTAAAATCAATGTGGAGGAGTCCCAATGGCACAATTAGAAACATTCTAAACGGTACTGTCTTTCGTGAACCTATCCTATGCCGAAACATTCCTAGAATAGTTCCTGGCTGGAAGAAACCCATCTGTATTGGTAGGCATGCTTTCGGCGATCAGTATCGAGCTACTGATACAGTTATCATGGGACCAGGAAAGCTGAAAATGGTATTTGTTCCAGAAGGAGGAGACACCCCTACAGAGCTTAATGTCTATGATTTCAAAGGTCCTGGTGTGGCCCTCGCTATGTACAATGTTGATGAGTCTATTCGAGCTTTTGCCGACTCTTCCATGTCGATGGCATTTTCCAAGAAGTGGCCACTATACTTGAGCACCAAAAACACAATTCTTAAGAAATATGATGGCAGGTTTAAGGACATATTCGAGGAGGTTTATGTGGAAAAGTGGAAGCAGAAGTTTGAAGAAAACTCTATATGGTATGAGCATCGGCTAATAGATGACATGGTGGCTTATGCACTCAAAAGTGAGGGTGGATATGTTTGGGCTTGCAAAAACTATGATGGAGATGTCCAGAGTGATTTGCTTGCTCAAGGATTTGGCTCTTTGGGCCTCATGACTTCTGTGCTGCTATCCTCAGATGGGAAAACAATAGAAGCTGAAGCAGCTCATGGGACAGTAACTCGTCATTTTCGGTTACATCAGAAGGGACAAGAAACCAGTACAAACAGTATTGCTTCAATATTTGCATGGACAAGAGGCCTAGAGCATAGGGGCAAATTAGATAAAAATGACAGGTTGCTAGATTTTGTTCGAAAGTTGGAGGCTGCATGCATTGAGACAGTGGAGGCAGGAAACATGACTAAGGATCTCGCCTTATTGATCCATGGCCCCAAGGTATCAAGGGAGTTCTATTTGAACACAGAAGAGTTTATTGACACAGTGGCAAAGAATGTTGAGCAAAAGCTTCGGGAACCTGCTCTGGTCTAA

Protein Analysis

480

Amino Acids

54.1

Weight (kDa)

8.51

Isoelectric Point (pI)

30.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Iso_dh PF00180 73 - 466 1.2e-73 Isocitrate/isopropylmalate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 852, 1438
AccB7I CCANNNNNTGG 1 cut(s) 810
AccII CGCG 3 cut(s) 37, 39, 138
AciI CCGC 3 cut(s) 42, 136, 150
AclWI GGATC 3 cut(s) 269, 1329, 1330
AcoI YGGCCR 1 cut(s) 812
AcuI CTGAAG 1 cut(s) 1134
AfaI GTAC 4 cut(s) 401, 517, 746, 1177
AfiI CCNNNNNNNGG 5 cut(s) 111, 304, 680, 810, 1095
AflII CTTAAG 2 cut(s) 395, 845
AgsI TTSAA 5 cut(s) 121, 718, 917, 1194, 1372
AjnI CCWGG 3 cut(s) 565, 646, 724
AluBI AGCT 8 cut(s) 90, 623, 655, 700, 770, 1112, 1121, 1420
AluI AGCT 8 cut(s) 90, 623, 655, 700, 770, 1112, 1121, 1420
Alw21I GWGCWC 1 cut(s) 830
Alw26I GTCTC 5 cut(s) 7, 145, 434, 678, 1286
AlwI GGATC 3 cut(s) 269, 1329, 1330
Ama87I CYCGRG 1 cut(s) 76
AoxI GGCC 5 cut(s) 732, 812, 1063, 1213, 1342
ApeKI GCWGC 4 cut(s) 185, 1081, 1118, 1280
AspLEI GCGC 4 cut(s) 29, 39, 41, 117
AspS9I GGNCC 5 cut(s) 644, 722, 733, 1063, 1343
AsuC2I CCSGG 1 cut(s) 215
AsuHPI GGTGA 1 cut(s) 257
AsuII TTCGAA 1 cut(s) 1267
AvaI CYCGRG 1 cut(s) 76
AvaII GGWCC 2 cut(s) 644, 722
BalI TGGCCA 1 cut(s) 814
BbsI GAAGAC 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 830
BbvI GCAGC 4 cut(s) 172, 1068, 1130, 1267
BccI CCATC 6 cut(s) 191, 590, 787, 851, 1010, 1088
BcgI CGANNNNNNTGC 2 cut(s) 1403, 1437
BciT130I CCWGG 3 cut(s) 567, 648, 726
BcnI CCSGG 1 cut(s) 215
BcoDI GTCTC 5 cut(s) 7, 145, 434, 678, 1286
BfaI CTAG 4 cut(s) 87, 555, 1217, 1256
BfmI CTRYAG 2 cut(s) 69, 693
BfoI RGCGCY 2 cut(s) 30, 118
BfrI CTTAAG 2 cut(s) 395, 845
BfuAI ACCTGC 2 cut(s) 852, 1438
BisI GCNGC 6 cut(s) 42, 150, 186, 1082, 1119, 1281
BlsI GCNGC 6 cut(s) 43, 151, 187, 1083, 1120, 1282
Bme1390I CCNGG 4 cut(s) 215, 567, 648, 726
Bme18I GGWCC 2 cut(s) 644, 722
BmeT110I CYCGRG 1 cut(s) 76
BmgT120I GGNCC 5 cut(s) 644, 722, 733, 1063, 1343
BmiI GGNNCC 3 cut(s) 645, 1345, 1428
BmrFI CCNGG 4 cut(s) 215, 567, 648, 726
BmsI GCATC 2 cut(s) 340, 949
BpiI GAAGAC 1 cut(s) 57
Bpu14I TTCGAA 1 cut(s) 1267
BpuEI CTTGAG 2 cut(s) 844, 1029
BpuMI CCSGG 1 cut(s) 215
BsaBI GATNNNNATC 1 cut(s) 615
BsaI GGTCTC 1 cut(s) 434
BsaJI CCNNGG 3 cut(s) 110, 1339, 1347
BsaWI WCCGGW 1 cut(s) 174
BsaXI ACNNNNNCTCC 2 cut(s) 415, 445
Bsc4I CCNNNNNNNGG 5 cut(s) 111, 304, 680, 810, 1095
Bse1I ACTGG 1 cut(s) 1173
Bse8I GATNNNNATC 1 cut(s) 615
BseBI CCWGG 3 cut(s) 567, 648, 726
BseDI CCNNGG 3 cut(s) 110, 1339, 1347
BseJI GATNNNNATC 1 cut(s) 615
BseLI CCNNNNNNNGG 5 cut(s) 111, 304, 680, 810, 1095
BseMII CTCAG 1 cut(s) 1104
BseNI ACTGG 1 cut(s) 1173
BsePI GCGCGC 1 cut(s) 37
BseRI GAGGAG 3 cut(s) 493, 696, 896
BseXI GCAGC 4 cut(s) 172, 1068, 1130, 1267
Bsh1236I CGCG 3 cut(s) 37, 39, 138
Bsh1285I CGRYCG 1 cut(s) 231
BshFI GGCC 5 cut(s) 734, 814, 1065, 1215, 1344
BsiEI CGRYCG 1 cut(s) 231
BsiHKAI GWGCWC 1 cut(s) 830
BsiHKCI CYCGRG 1 cut(s) 76
BsiSI CCGG 2 cut(s) 175, 214
BslFI GGGAC 4 cut(s) 468, 657, 1141, 1176
BslI CCNNNNNNNGG 5 cut(s) 111, 304, 680, 810, 1095
BsmAI GTCTC 5 cut(s) 7, 145, 434, 678, 1286
BsmBI CGTCTC 1 cut(s) 145
BsmFI GGGAC 4 cut(s) 468, 657, 1141, 1176
BsnI GGCC 5 cut(s) 734, 814, 1065, 1215, 1344
Bso31I GGTCTC 1 cut(s) 434
BsoBI CYCGRG 1 cut(s) 76
Bsp119I TTCGAA 1 cut(s) 1267
Bsp1286I GDGCHC 1 cut(s) 830
Bsp1407I TGTACA 1 cut(s) 744
Bsp143I GATC 5 cut(s) 228, 261, 610, 1321, 1335
Bsp19I CCATGG 1 cut(s) 1339
BspACI CCGC 3 cut(s) 42, 136, 150
BspANI GGCC 5 cut(s) 734, 814, 1065, 1215, 1344
BspCNI CTCAG 1 cut(s) 1103
BspFNI CGCG 3 cut(s) 37, 39, 138
BspHI TCATGA 1 cut(s) 1068
BspLI GGNNCC 3 cut(s) 645, 1345, 1428
BspMI ACCTGC 2 cut(s) 852, 1438
BspPI GGATC 3 cut(s) 269, 1329, 1330
BspT104I TTCGAA 1 cut(s) 1267
BspTI CTTAAG 2 cut(s) 395, 845
BspTNI GGTCTC 1 cut(s) 434
BsrGI TGTACA 1 cut(s) 744
BsrI ACTGG 1 cut(s) 1173
BssECI CCNNGG 3 cut(s) 110, 1339, 1347
BssHII GCGCGC 1 cut(s) 37
BssMI GATC 5 cut(s) 228, 261, 610, 1321, 1335
BssT1I CCWWGG 2 cut(s) 1339, 1347
Bst2UI CCWGG 3 cut(s) 567, 648, 726
Bst6I CTCTTC 2 cut(s) 787, 1374
BstAFI CTTAAG 2 cut(s) 395, 845
BstAUI TGTACA 1 cut(s) 744
BstBI TTCGAA 1 cut(s) 1267
BstC8I GCNNGC 5 cut(s) 39, 600, 1003, 1041, 1285
BstDEI CTNAG 2 cut(s) 1090, 1317
BstDSI CCRYGG 1 cut(s) 1339
BstFNI CGCG 3 cut(s) 37, 39, 138
BstH2I RGCGCY 2 cut(s) 30, 118
BstHHI GCGC 4 cut(s) 29, 39, 41, 117
BstKTI GATC 5 cut(s) 231, 264, 613, 1324, 1338
BstMAI GTCTC 5 cut(s) 7, 145, 434, 678, 1286
BstMBI GATC 5 cut(s) 228, 261, 610, 1321, 1335
BstMCI CGRYCG 1 cut(s) 231
BstMWI GCNNNNNNNGC 2 cut(s) 1062, 1118
BstNI CCWGG 3 cut(s) 567, 648, 726
BstNSI RCATGY 2 cut(s) 602, 1287
BstSCI CCNGG 4 cut(s) 213, 565, 646, 724
BstSFI CTRYAG 2 cut(s) 69, 693
BstUI CGCG 3 cut(s) 37, 39, 138
BstV1I GCAGC 4 cut(s) 172, 1068, 1130, 1267
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 1 cut(s) 1321
BstXI CCANNNNNNTGG 1 cut(s) 793
BstYI RGATCY 1 cut(s) 1321
BsuRI GGCC 5 cut(s) 734, 814, 1065, 1215, 1344
BtgI CCRYGG 1 cut(s) 1339
BtsIMutI CAGTG 2 cut(s) 1302, 1401
BveI ACCTGC 2 cut(s) 852, 1438
Cac8I GCNNGC 5 cut(s) 39, 600, 1003, 1041, 1285
CciI TCATGA 1 cut(s) 1068
CfoI GCGC 4 cut(s) 29, 39, 41, 117
Cfr13I GGNCC 5 cut(s) 644, 722, 733, 1063, 1343
CseI GACGC 1 cut(s) 127
Csp6I GTAC 4 cut(s) 400, 516, 745, 1176
CspCI CAANNNNNGTGG 2 cut(s) 804, 839
CviQI GTAC 4 cut(s) 400, 516, 745, 1176
DdeI CTNAG 2 cut(s) 1090, 1317
DpnI GATC 5 cut(s) 230, 263, 612, 1323, 1337
DpnII GATC 5 cut(s) 228, 261, 610, 1321, 1335
EaeI YGGCCR 1 cut(s) 812
Eam1104I CTCTTC 2 cut(s) 787, 1374
EarI CTCTTC 2 cut(s) 787, 1374
Eco130I CCWWGG 2 cut(s) 1339, 1347
Eco147I AGGCCT 1 cut(s) 1215
Eco31I GGTCTC 1 cut(s) 434
Eco32I GATATC 1 cut(s) 51
Eco47I GGWCC 2 cut(s) 644, 722
Eco57I CTGAAG 1 cut(s) 1134
Eco88I CYCGRG 1 cut(s) 76
EcoO109I RGGNCCY 1 cut(s) 722
EcoRII CCWGG 3 cut(s) 565, 646, 724
EcoRV GATATC 1 cut(s) 51
EcoT14I CCWWGG 2 cut(s) 1339, 1347
EcoT22I ATGCAT 1 cut(s) 1289
ErhI CCWWGG 2 cut(s) 1339, 1347
Esp3I CGTCTC 1 cut(s) 145
FalI AAGNNNNNCTT 4 cut(s) 273, 305, 378, 410
FaqI GGGAC 4 cut(s) 468, 657, 1141, 1176
FauNDI CATATG 1 cut(s) 266
Fnu4HI GCNGC 6 cut(s) 42, 150, 186, 1082, 1119, 1281
Fsp4HI GCNGC 6 cut(s) 42, 150, 186, 1082, 1119, 1281
FspBI CTAG 4 cut(s) 87, 555, 1217, 1256
GlaI GCGC 4 cut(s) 28, 38, 40, 116
GluI GCNGC 6 cut(s) 42, 150, 186, 1082, 1119, 1281
HaeII RGCGCY 2 cut(s) 30, 118
HaeIII GGCC 5 cut(s) 734, 814, 1065, 1215, 1344
HapII CCGG 2 cut(s) 175, 214
HgaI GACGC 1 cut(s) 127
HhaI GCGC 4 cut(s) 29, 39, 41, 117
Hin6I GCGC 4 cut(s) 27, 37, 39, 115
HinP1I GCGC 4 cut(s) 27, 37, 39, 115
HindIII AAGCTT 1 cut(s) 1418
HinfI GANTC 6 cut(s) 15, 126, 223, 481, 758, 779
HpaII CCGG 2 cut(s) 175, 214
HphI GGTGA 1 cut(s) 257
Hpy166II GTNNAC 1 cut(s) 530
Hpy188I TCNGA 5 cut(s) 196, 222, 228, 1093, 1158
Hpy188III TCNNGA 7 cut(s) 205, 434, 527, 674, 1027, 1069, 1424
Hpy8I GTNNAC 1 cut(s) 530
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 4 cut(s) 106, 445, 671, 1153
HpyCH4V TGCA 5 cut(s) 971, 1005, 1202, 1283, 1287
HpyF10VI GCNNNNNNNGC 2 cut(s) 1062, 1118
HpyF3I CTNAG 2 cut(s) 1090, 1317
HspAI GCGC 4 cut(s) 27, 37, 39, 115
Kzo9I GATC 5 cut(s) 228, 261, 610, 1321, 1335
LmnI GCTCC 1 cut(s) 177
Lsp1109I GCAGC 4 cut(s) 172, 1068, 1130, 1267
LweI GCATC 2 cut(s) 340, 949
MaeI CTAG 4 cut(s) 87, 555, 1217, 1256
MaeIII GTNAC 3 cut(s) 367, 1132, 1149
MalI GATC 5 cut(s) 230, 263, 612, 1323, 1337
MboI GATC 5 cut(s) 228, 261, 610, 1321, 1335
MboII GAAGA 8 cut(s) 45, 48, 51, 57, 586, 774, 929, 1391
MflI RGATCY 1 cut(s) 1321
MhlI GDGCHC 1 cut(s) 830
MlsI TGGCCA 1 cut(s) 814
MluCI AATT 5 cut(s) 20, 276, 495, 840, 1232
MluNI TGGCCA 1 cut(s) 814
MlyI GAGTC 5 cut(s) 9, 135, 490, 767, 773
MmeI TCCRAC 1 cut(s) 1254
Mox20I TGGCCA 1 cut(s) 814
Mph1103I ATGCAT 1 cut(s) 1289
MscI TGGCCA 1 cut(s) 814
MseI TTAA 5 cut(s) 279, 396, 702, 846, 867
MslI CAYNNNNRTG 1 cut(s) 791
Msp20I TGGCCA 1 cut(s) 814
MspCI CTTAAG 2 cut(s) 395, 845
MspI CCGG 2 cut(s) 175, 214
MspR9I CCNGG 4 cut(s) 215, 567, 648, 726
MvaI CCWGG 3 cut(s) 567, 648, 726
MvnI CGCG 3 cut(s) 37, 39, 138
MwoI GCNNNNNNNGC 2 cut(s) 1062, 1118
NciI CCSGG 1 cut(s) 215
NcoI CCATGG 1 cut(s) 1339
NdeI CATATG 1 cut(s) 266
NdeII GATC 5 cut(s) 228, 261, 610, 1321, 1335
NlaIV GGNNCC 3 cut(s) 645, 1345, 1428
NmeAIII GCCGAG 1 cut(s) 135
NsiI ATGCAT 1 cut(s) 1289
NspI RCATGY 2 cut(s) 602, 1287
NspV TTCGAA 1 cut(s) 1267
PaeI GCATGC 2 cut(s) 602, 1287
PagI TCATGA 1 cut(s) 1068
PauI GCGCGC 1 cut(s) 37
PceI AGGCCT 1 cut(s) 1215
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PfeI GAWTC 1 cut(s) 223
PflMI CCANNNNNTGG 1 cut(s) 810
PkrI GCNGC 6 cut(s) 43, 151, 187, 1083, 1120, 1282
Ple19I CGATCG 1 cut(s) 231
PleI GAGTC 5 cut(s) 9, 134, 489, 766, 773
PpsI GAGTC 5 cut(s) 9, 134, 489, 766, 773
PpuMI RGGWCCY 1 cut(s) 722
Psp5II RGGWCCY 1 cut(s) 722
Psp6I CCWGG 3 cut(s) 565, 646, 724
PspGI CCWGG 3 cut(s) 565, 646, 724
PspN4I GGNNCC 3 cut(s) 645, 1345, 1428
PspPI GGNCC 5 cut(s) 644, 722, 733, 1063, 1343
PspPPI RGGWCCY 1 cut(s) 722
PsuI RGATCY 1 cut(s) 1321
PteI GCGCGC 1 cut(s) 37
PvuI CGATCG 1 cut(s) 231
RsaI GTAC 4 cut(s) 401, 517, 746, 1177
RsaNI GTAC 4 cut(s) 400, 516, 745, 1176
RseI CAYNNNNRTG 1 cut(s) 791
SaqAI TTAA 5 cut(s) 279, 396, 702, 846, 867
SatI GCNGC 6 cut(s) 42, 150, 186, 1082, 1119, 1281
Sau3AI GATC 5 cut(s) 228, 261, 610, 1321, 1335
Sau96I GGNCC 5 cut(s) 644, 722, 733, 1063, 1343
SchI GAGTC 5 cut(s) 9, 135, 490, 767, 773
ScrFI CCNGG 4 cut(s) 215, 567, 648, 726
SduI GDGCHC 1 cut(s) 830
SfaNI GCATC 2 cut(s) 340, 949
SfcI CTRYAG 2 cut(s) 69, 693
SfuI TTCGAA 1 cut(s) 1267
SinI GGWCC 2 cut(s) 644, 722
SmiMI CAYNNNNRTG 1 cut(s) 791
SmlI CTYRAG 4 cut(s) 395, 823, 845, 1044
SmoI CTYRAG 4 cut(s) 395, 823, 845, 1044
SphI GCATGC 2 cut(s) 602, 1287
Sse9I AATT 5 cut(s) 20, 276, 495, 840, 1232
SseBI AGGCCT 1 cut(s) 1215
SsiI CCGC 3 cut(s) 42, 136, 150
SspI AATATT 1 cut(s) 1197
SspMI CTAG 4 cut(s) 87, 555, 1217, 1256
StuI AGGCCT 1 cut(s) 1215
StyD4I CCNGG 4 cut(s) 213, 565, 646, 724
StyI CCWWGG 2 cut(s) 1339, 1347
TaqI TCGA 7 cut(s) 204, 234, 619, 766, 791, 879, 1267
TasI AATT 5 cut(s) 20, 276, 495, 840, 1232
TatI WGTACW 3 cut(s) 399, 744, 1175
TauI GCSGC 2 cut(s) 44, 152
TfiI GAWTC 1 cut(s) 223
Tru1I TTAA 5 cut(s) 279, 396, 702, 846, 867
Tru9I TTAA 5 cut(s) 279, 396, 702, 846, 867
TscAI CASTG 2 cut(s) 1302, 1401
TseI GCWGC 4 cut(s) 185, 1081, 1118, 1280
TspDTI ATGAA 2 cut(s) 264, 464
TspRI CASTG 2 cut(s) 1302, 1401
Van91I CCANNNNNTGG 1 cut(s) 810
Vha464I CTTAAG 2 cut(s) 395, 845
VpaK11BI GGWCC 2 cut(s) 644, 722
XceI RCATGY 2 cut(s) 602, 1287
XspI CTAG 4 cut(s) 87, 555, 1217, 1256
Zsp2I ATGCAT 1 cut(s) 1289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.