MD09G1032600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012020
Physical Location & Seq
Reverse (-)
1976548 .. 1980607
4060 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1032600.v1.1.491

Sequence Viewer

Length: 1314 bp
ATGGGGAACTTGCTGGACAAGGAACCACCGCCGCCCATGGTGCTCGTCCCGCCGCTCTTCGACTACCCTCCGCTCGCGGCCCGCACCAGGATGTTGGAGTCGTCGTATGACTTGTTGTTTGGGAAACTCGCTCTGAAATGTCTGTTTGACGATTACTTTGAACAAGCTCGGCATTTCAGCACATTGATCATGCTGAAGCCCATCGACGATCCTCACGTCGATTTGGTTGCAACTGTTTCAGGACCGCTCGATCACAAACCCGAGGAGAATATAGTTGGAAATGCATTTTTTCGCTGGCAAAGTGATGTTGATGATCCTCACACATTCATGGACCTCTTTGTATCGAACACTGATCCGGTTTTGCAGATGAGAGCCACTGGGAGCGTTTGGAATTTTCCCAATGTTGCTGAAAAAAAGGTAATAACTTCTGAAGACTTTGGTGTTATGGGATTGAGATATGGCTCGTCAAACTTGTCAATTGGAGCCACTGTCATGCCCTTCTCCTTGAAAGATGATTGTCCAAGAAGGGCATGGTTGGTGAGCAAGATGGGAAGACTAACAGCTGGGGTGCAGTATGAGCCTGAATATGGGAGTAAAGATACTCAAAACTACAAAAATTTAAAGAATTGGAGTGCTGCAATCGGTTATGGAGTGGGATCGAGCAGCCCCTTGAGTCCATCATTCAATTTTGGTCTGGAGTTATCAAGAAGTTCTCAGTTCATTGCCTCATTCTACCAACACGTGGTGGTCCAAAGACGGGTGAAAAACCCCCTTGAAGAAAATGAAGTGGTTGGAATTACGAATTATATAGATTTTGGCTTTGAATTGCAGACAAGGGTTGATGATATCAAATCATCAAGCGATGTCCCAGAATCAAACGATGTTCCAGAATCAAGCGATGTACCTAAATCGAGCAATGGACCTAATGTCCAAGATTCCACCTTTCAAGTAGCTGCATCTTGGCAAGCCAATAAGAACTTCATGCTCAAGGGAAAGGTGGGCCCTCTCAGCTCATCGATAGCATTGGCATTTAAATCCTGGTGGAAACCTTCTTTCACATTCAGCGTTTCAGCTACGAGAAATCACATTGTTGGGGAGACAGGTTATGGTTTCGGCCTTCGCGTGGAGAATCTTAGACAAGCCAGTTATCAAAGAGCTGATCCGAATTTCGTCATGTTGACACCGAATAAGGAGCACCTGGCGGAAGGCATTGTTTGGAAAGCAGGGAAAAGACCGATGCTGCAGTCAGATATAAATGCCGGAAATTTCGACGGCATTCCAAAGGAACTAAGACCCCTGGGGAAAATCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

438

Amino Acids

48.67

Weight (kDa)

5.88

Isoelectric Point (pI)

39.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012020)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27930 AT3G27930 AT3G27930
fragaria_vesca FvH4_6g50760
malus_domestica MD09G1032600.v1.1 MD17G1034200.v1.1
prunus_persica Prupe.3G285400_v2.0.a1
pyrus_communis pycom17g03160
rosa_chinensis RchiOBHm_Chr2g0171701
rosa_laevigata RLG00000022030
rosa_multiflora Rmu_ssc0000252.1_g000012
rosa_roxburghii Rroxscaffold_2G00080510
rosa_rugosa Rorug02G0555400
rosa_samantha Rh2BG641000 Rh2BG643500 Rh2CG609200 Rh2DG651900 Rh2DG657200
rosa_wichuraiana Rw2G052180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 742
AccBSI CCGCTC 3 cut(s) 55, 73, 247
AccII CGCG 2 cut(s) 77, 1122
AciI CCGC 9 cut(s) 29, 32, 50, 53, 71, 77, 82, 245, 1202
AclWI GGATC 5 cut(s) 203, 308, 347, 664, 1154
AcsI RAATTY 4 cut(s) 391, 616, 1165, 1264
AcuI CTGAAG 2 cut(s) 215, 450
AcvI CACGTG 1 cut(s) 742
AdeI CACNNNGTG 1 cut(s) 745
AfaI GTAC 1 cut(s) 903
AfiI CCNNNNNNNGG 5 cut(s) 87, 587, 742, 757, 1123
AflIII ACRYGT 1 cut(s) 739
AgsI TTSAA 6 cut(s) 161, 508, 685, 776, 824, 947
AhdI GACNNNNNGTC 1 cut(s) 926
AjiI CACGTC 1 cut(s) 217
AjnI CCWGG 4 cut(s) 86, 1037, 1197, 1296
AjuI GAANNNNNNNTTGG 2 cut(s) 962, 994
AluBI AGCT 6 cut(s) 167, 563, 953, 1011, 1073, 1157
AluI AGCT 6 cut(s) 167, 563, 953, 1011, 1073, 1157
Alw21I GWGCWC 2 cut(s) 45, 1197
Alw26I GTCTC 1 cut(s) 1091
AlwI GGATC 5 cut(s) 203, 308, 347, 664, 1154
Ama87I CYCGRG 1 cut(s) 260
AoxI GGCC 3 cut(s) 78, 1000, 1114
ApaI GGGCCC 1 cut(s) 1004
ApeKI GCWGC 4 cut(s) 635, 663, 953, 1240
ApoI RAATTY 4 cut(s) 391, 616, 1165, 1264
ArsI GACNNNNNNTTYG 4 cut(s) 101, 133, 140, 172
AspS9I GGNCC 7 cut(s) 79, 242, 331, 748, 920, 1000, 1001
AsuHPI GGTGA 2 cut(s) 550, 772
AvaI CYCGRG 1 cut(s) 260
AvaII GGWCC 4 cut(s) 242, 331, 748, 920
BaeGI GKGCMC 1 cut(s) 1004
BanII GRGCYC 1 cut(s) 1004
BbrPI CACGTG 1 cut(s) 742
BbsI GAAGAC 2 cut(s) 438, 559
Bbv12I GWGCWC 2 cut(s) 45, 1197
BbvI GCAGC 4 cut(s) 622, 675, 940, 1227
BccI CCATC 3 cut(s) 209, 541, 685
BceAI ACGGC 1 cut(s) 1288
BcgI CGANNNNNNTGC 2 cut(s) 209, 243
BciT130I CCWGG 4 cut(s) 88, 1039, 1199, 1298
BclI TGATCA 1 cut(s) 186
BcoDI GTCTC 1 cut(s) 1091
BfmI CTRYAG 1 cut(s) 1241
BisI GCNGC 7 cut(s) 32, 53, 78, 636, 664, 954, 1241
BlsI GCNGC 7 cut(s) 33, 54, 79, 637, 665, 955, 1242
Bme1390I CCNGG 4 cut(s) 88, 1039, 1199, 1298
Bme18I GGWCC 4 cut(s) 242, 331, 748, 920
BmeRI GACNNNNNGTC 1 cut(s) 926
BmeT110I CYCGRG 1 cut(s) 260
BmgBI CACGTC 1 cut(s) 217
BmgT120I GGNCC 7 cut(s) 79, 242, 331, 748, 920, 1000, 1001
BmiI GGNNCC 3 cut(s) 24, 484, 1002
BmrFI CCNGG 4 cut(s) 88, 1039, 1199, 1298
BmrI ACTGGG 1 cut(s) 387
BmsI GCATC 2 cut(s) 965, 1227
BmuI ACTGGG 1 cut(s) 387
BpiI GAAGAC 2 cut(s) 438, 559
BpmI CTGGAG 1 cut(s) 716
BpuEI CTTGAG 2 cut(s) 691, 971
Bsa29I ATCGAT 1 cut(s) 1016
BsaAI YACGTR 1 cut(s) 742
BsaJI CCNNGG 4 cut(s) 36, 261, 1296, 1297
BsaWI WCCGGW 1 cut(s) 355
BsaXI ACNNNNNCTCC 4 cut(s) 89, 119, 474, 504
Bsc4I CCNNNNNNNGG 5 cut(s) 87, 587, 742, 757, 1123
Bse1I ACTGG 2 cut(s) 382, 1143
Bse3DI GCAATG 2 cut(s) 720, 922
BseBI CCWGG 4 cut(s) 88, 1039, 1199, 1298
BseCI ATCGAT 1 cut(s) 1016
BseDI CCNNGG 4 cut(s) 36, 261, 1296, 1297
BseGI GGATG 1 cut(s) 96
BseLI CCNNNNNNNGG 5 cut(s) 87, 587, 742, 757, 1123
BseMI GCAATG 2 cut(s) 720, 922
BseMII CTCAG 2 cut(s) 728, 1021
BseNI ACTGG 2 cut(s) 382, 1143
BseRI GAGGAG 1 cut(s) 278
BseSI GKGCMC 1 cut(s) 1004
BseXI GCAGC 4 cut(s) 622, 675, 940, 1227
BseYI CCCAGC 1 cut(s) 563
BsgI GTGCAG 1 cut(s) 590
Bsh1236I CGCG 2 cut(s) 77, 1122
BshFI GGCC 3 cut(s) 80, 1002, 1116
BshVI ATCGAT 1 cut(s) 1016
BsiHKAI GWGCWC 2 cut(s) 45, 1197
BsiHKCI CYCGRG 1 cut(s) 260
BsiSI CCGG 2 cut(s) 356, 1260
BslFI GGGAC 2 cut(s) 32, 851
BslI CCNNNNNNNGG 5 cut(s) 87, 587, 742, 757, 1123
BsmAI GTCTC 1 cut(s) 1091
BsmFI GGGAC 2 cut(s) 32, 851
BsmI GAATGC 1 cut(s) 1275
BsnI GGCC 3 cut(s) 80, 1002, 1116
BsoBI CYCGRG 1 cut(s) 260
Bsp120I GGGCCC 1 cut(s) 1000
Bsp1286I GDGCHC 3 cut(s) 45, 1004, 1197
Bsp143I GATC 7 cut(s) 186, 208, 250, 313, 352, 656, 1159
Bsp19I CCATGG 1 cut(s) 36
BspACI CCGC 9 cut(s) 29, 32, 50, 53, 71, 77, 82, 245, 1202
BspANI GGCC 3 cut(s) 80, 1002, 1116
BspCNI CTCAG 2 cut(s) 727, 1020
BspDI ATCGAT 1 cut(s) 1016
BspFNI CGCG 2 cut(s) 77, 1122
BspLI GGNNCC 3 cut(s) 24, 484, 1002
BspMAI CTGCAG 1 cut(s) 1245
BspPI GGATC 5 cut(s) 203, 308, 347, 664, 1154
BspQI GCTCTTC 1 cut(s) 62
BsrBI CCGCTC 3 cut(s) 55, 73, 247
BsrDI GCAATG 2 cut(s) 720, 922
BsrI ACTGG 2 cut(s) 382, 1143
BssECI CCNNGG 4 cut(s) 36, 261, 1296, 1297
BssMI GATC 7 cut(s) 186, 208, 250, 313, 352, 656, 1159
BssT1I CCWWGG 1 cut(s) 36
Bst2UI CCWGG 4 cut(s) 88, 1039, 1199, 1298
Bst4CI ACNGT 2 cut(s) 235, 490
Bst6I CTCTTC 1 cut(s) 62
BstBAI YACGTR 1 cut(s) 742
BstC8I GCNNGC 4 cut(s) 75, 82, 296, 966
BstDEI CTNAG 4 cut(s) 714, 1007, 1133, 1289
BstDSI CCRYGG 1 cut(s) 36
BstF5I GGATG 1 cut(s) 96
BstFNI CGCG 2 cut(s) 77, 1122
BstKTI GATC 7 cut(s) 189, 211, 253, 316, 355, 659, 1162
BstMAI GTCTC 1 cut(s) 1091
BstMBI GATC 7 cut(s) 186, 208, 250, 313, 352, 656, 1159
BstMWI GCNNNNNNNGC 4 cut(s) 40, 49, 577, 1008
BstNI CCWGG 4 cut(s) 88, 1039, 1199, 1298
BstSCI CCNGG 4 cut(s) 86, 1037, 1197, 1296
BstSFI CTRYAG 1 cut(s) 1241
BstSLI GKGCMC 1 cut(s) 1004
BstUI CGCG 2 cut(s) 77, 1122
BstV1I GCAGC 4 cut(s) 622, 675, 940, 1227
BstV2I GAAGAC 2 cut(s) 438, 559
BstXI CCANNNNNNTGG 1 cut(s) 94
Bsu15I ATCGAT 1 cut(s) 1016
BsuRI GGCC 3 cut(s) 80, 1002, 1116
BsuTUI ATCGAT 1 cut(s) 1016
BtgI CCRYGG 1 cut(s) 36
BtgZI GCGATG 2 cut(s) 876, 912
BtrI CACGTC 1 cut(s) 217
BtsCI GGATG 1 cut(s) 96
BtsIMutI CAGTG 3 cut(s) 348, 375, 486
Cac8I GCNNGC 4 cut(s) 75, 82, 296, 966
Cfr13I GGNCC 7 cut(s) 79, 242, 331, 748, 920, 1000, 1001
ClaI ATCGAT 1 cut(s) 1016
Csp6I GTAC 1 cut(s) 902
CviAII CATG 7 cut(s) 37, 190, 328, 493, 531, 982, 1174
CviQI GTAC 1 cut(s) 902
DdeI CTNAG 4 cut(s) 714, 1007, 1133, 1289
DpnI GATC 7 cut(s) 188, 210, 252, 315, 354, 658, 1161
DpnII GATC 7 cut(s) 186, 208, 250, 313, 352, 656, 1159
DraI TTTAAA 2 cut(s) 621, 1033
DraIII CACNNNGTG 1 cut(s) 745
DriI GACNNNNNGTC 1 cut(s) 926
Eam1104I CTCTTC 1 cut(s) 62
Eam1105I GACNNNNNGTC 1 cut(s) 926
EarI CTCTTC 1 cut(s) 62
EciI GGCGGA 1 cut(s) 1217
Eco130I CCWWGG 1 cut(s) 36
Eco24I GRGCYC 1 cut(s) 1004
Eco32I GATATC 1 cut(s) 847
Eco47I GGWCC 4 cut(s) 242, 331, 748, 920
Eco57I CTGAAG 2 cut(s) 215, 450
Eco72I CACGTG 1 cut(s) 742
Eco88I CYCGRG 1 cut(s) 260
EcoO109I RGGNCCY 1 cut(s) 1001
EcoRII CCWGG 4 cut(s) 86, 1037, 1197, 1296
EcoRV GATATC 1 cut(s) 847
EcoT14I CCWWGG 1 cut(s) 36
EcoT22I ATGCAT 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 1004
ErhI CCWWGG 1 cut(s) 36
FaeI CATG 7 cut(s) 40, 193, 331, 496, 534, 985, 1177
FaqI GGGAC 2 cut(s) 32, 851
FatI CATG 7 cut(s) 36, 189, 327, 492, 530, 981, 1173
FauI CCCGC 2 cut(s) 57, 89
FbaI TGATCA 1 cut(s) 186
Fnu4HI GCNGC 7 cut(s) 32, 53, 78, 636, 664, 954, 1241
FokI GGATG 1 cut(s) 103
FriOI GRGCYC 1 cut(s) 1004
Fsp4HI GCNGC 7 cut(s) 32, 53, 78, 636, 664, 954, 1241
GluI GCNGC 7 cut(s) 32, 53, 78, 636, 664, 954, 1241
GsaI CCCAGC 1 cut(s) 567
GsuI CTGGAG 1 cut(s) 716
HaeIII GGCC 3 cut(s) 80, 1002, 1116
HapII CCGG 2 cut(s) 356, 1260
Hin1II CATG 7 cut(s) 40, 193, 331, 496, 534, 985, 1177
HincII GTYRAC 1 cut(s) 1179
HindII GTYRAC 1 cut(s) 1179
HinfI GANTC 6 cut(s) 98, 673, 872, 890, 935, 1129
HpaII CCGG 2 cut(s) 356, 1260
HphI GGTGA 2 cut(s) 550, 772
Hpy166II GTNNAC 1 cut(s) 1179
Hpy188I TCNGA 4 cut(s) 135, 430, 1164, 1249
Hpy188III TCNNGA 4 cut(s) 240, 695, 705, 887
Hpy8I GTNNAC 1 cut(s) 1179
Hpy99I CGWCG 4 cut(s) 106, 209, 221, 1274
HpyAV CCTTC 5 cut(s) 508, 519, 1059, 1127, 1199
HpyCH4III ACNGT 2 cut(s) 235, 490
HpyCH4IV ACGT 2 cut(s) 216, 741
HpyCH4V TGCA 8 cut(s) 230, 284, 364, 571, 638, 829, 956, 1243
HpyF10VI GCNNNNNNNGC 4 cut(s) 40, 49, 577, 1008
HpyF3I CTNAG 4 cut(s) 714, 1007, 1133, 1289
HpySE526I ACGT 2 cut(s) 216, 741
Hsp92II CATG 7 cut(s) 40, 193, 331, 496, 534, 985, 1177
Ksp22I TGATCA 1 cut(s) 186
Kzo9I GATC 7 cut(s) 186, 208, 250, 313, 352, 656, 1159
LguI GCTCTTC 1 cut(s) 62
LmnI GCTCC 3 cut(s) 381, 482, 1192
Lsp1109I GCAGC 4 cut(s) 622, 675, 940, 1227
LweI GCATC 2 cut(s) 965, 1227
MaeII ACGT 2 cut(s) 216, 741
MalI GATC 7 cut(s) 188, 210, 252, 315, 354, 658, 1161
MbiI CCGCTC 3 cut(s) 55, 73, 247
MboI GATC 7 cut(s) 186, 208, 250, 313, 352, 656, 1159
MboII GAAGA 4 cut(s) 49, 443, 564, 788
MfeI CAATTG 1 cut(s) 477
MhlI GDGCHC 3 cut(s) 45, 1004, 1197
MlyI GAGTC 2 cut(s) 107, 682
MmeI TCCRAC 3 cut(s) 75, 256, 772
MnlI CCTC 7 cut(s) 78, 222, 256, 327, 344, 736, 1014
Mph1103I ATGCAT 1 cut(s) 286
MseI TTAA 2 cut(s) 620, 1032
MslI CAYNNNNRTG 3 cut(s) 89, 326, 491
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 2 cut(s) 356, 1260
MspR9I CCNGG 4 cut(s) 88, 1039, 1199, 1298
MunI CAATTG 1 cut(s) 477
Mva1269I GAATGC 1 cut(s) 1275
MvaI CCWGG 4 cut(s) 88, 1039, 1199, 1298
MvnI CGCG 2 cut(s) 77, 1122
MwoI GCNNNNNNNGC 4 cut(s) 40, 49, 577, 1008
NcoI CCATGG 1 cut(s) 36
NdeII GATC 7 cut(s) 186, 208, 250, 313, 352, 656, 1159
NlaIII CATG 7 cut(s) 40, 193, 331, 496, 534, 985, 1177
NlaIV GGNNCC 3 cut(s) 24, 484, 1002
NmeAIII GCCGAG 1 cut(s) 148
NsiI ATGCAT 1 cut(s) 286
PasI CCCWGGG 1 cut(s) 1297
PciSI GCTCTTC 1 cut(s) 62
PcsI WCGNNNNNNNCGW 2 cut(s) 213, 1119
PctI GAATGC 1 cut(s) 1275
PfeI GAWTC 4 cut(s) 872, 890, 935, 1129
PflMI CCANNNNNTGG 1 cut(s) 742
PkrI GCNGC 7 cut(s) 33, 54, 79, 637, 665, 955, 1242
PleI GAGTC 2 cut(s) 106, 681
PmaCI CACGTG 1 cut(s) 742
PmlI CACGTG 1 cut(s) 742
PpsI GAGTC 2 cut(s) 106, 681
Ppu21I YACGTR 1 cut(s) 742
Psp6I CCWGG 4 cut(s) 86, 1037, 1197, 1296
PspCI CACGTG 1 cut(s) 742
PspFI CCCAGC 1 cut(s) 563
PspGI CCWGG 4 cut(s) 86, 1037, 1197, 1296
PspN4I GGNNCC 3 cut(s) 24, 484, 1002
PspOMI GGGCCC 1 cut(s) 1000
PspPI GGNCC 7 cut(s) 79, 242, 331, 748, 920, 1000, 1001
PstI CTGCAG 1 cut(s) 1245
PvuII CAGCTG 1 cut(s) 563
RsaI GTAC 1 cut(s) 903
RsaNI GTAC 1 cut(s) 902
RseI CAYNNNNRTG 3 cut(s) 89, 326, 491
SapI GCTCTTC 1 cut(s) 62
SaqAI TTAA 2 cut(s) 620, 1032
SatI GCNGC 7 cut(s) 32, 53, 78, 636, 664, 954, 1241
Sau3AI GATC 7 cut(s) 186, 208, 250, 313, 352, 656, 1159
Sau96I GGNCC 7 cut(s) 79, 242, 331, 748, 920, 1000, 1001
SchI GAGTC 2 cut(s) 107, 682
ScrFI CCNGG 4 cut(s) 88, 1039, 1199, 1298
SduI GDGCHC 3 cut(s) 45, 1004, 1197
SfaNI GCATC 2 cut(s) 965, 1227
SfcI CTRYAG 1 cut(s) 1241
SinI GGWCC 4 cut(s) 242, 331, 748, 920
SmiI ATTTAAAT 1 cut(s) 1033
SmiMI CAYNNNNRTG 3 cut(s) 89, 326, 491
SmlI CTYRAG 2 cut(s) 670, 986
SmoI CTYRAG 2 cut(s) 670, 986
SsiI CCGC 9 cut(s) 29, 32, 50, 53, 71, 77, 82, 245, 1202
StyD4I CCNGG 4 cut(s) 86, 1037, 1197, 1296
StyI CCWWGG 1 cut(s) 36
SwaI ATTTAAAT 1 cut(s) 1033
TaaI ACNGT 2 cut(s) 235, 490
TaiI ACGT 2 cut(s) 219, 744
TaqI TCGA 9 cut(s) 60, 204, 219, 249, 344, 659, 911, 1016, 1269
TaqII GACCGA 1 cut(s) 1249
TauI GCSGC 3 cut(s) 34, 55, 80
TfiI GAWTC 4 cut(s) 872, 890, 935, 1129
Tru1I TTAA 2 cut(s) 620, 1032
Tru9I TTAA 2 cut(s) 620, 1032
TscAI CASTG 3 cut(s) 355, 382, 493
TseI GCWGC 4 cut(s) 635, 663, 953, 1240
TspDTI ATGAA 4 cut(s) 316, 709, 798, 970
TspRI CASTG 3 cut(s) 355, 382, 493
Van91I CCANNNNNTGG 1 cut(s) 742
VpaK11BI GGWCC 4 cut(s) 242, 331, 748, 920
XapI RAATTY 4 cut(s) 391, 616, 1165, 1264
XcmI CCANNNNNNNNNTGG 1 cut(s) 528
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.