MD17G1034200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0012020
Physical Location & Seq
Reverse (-)
2470970 .. 2475122
4153 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1034200.v1.1.491

Sequence Viewer

Length: 1335 bp
ATGGGAAACTTGCTGGACAAGGAGCCACCGCCGCCGATGGTGCTCGTACCGCCGCTTTTCGACTACCCTCCTCTCGCCGCCCGCACCAGGATGTTGGAGTCGTCGTATGACTTGTTGTTTGGGAAGCTTGCTTTGAAATGTCTGTTTGACGATTACTTTGAACAAGCTCGGCATTTCAGCACAATGATCATGCTGAAGCCCATCGACGACCCTCACGTCGATTTGGTTGCAACTGTTGCAGGACCGCTCGATCACAAACCCGAGGAGAATATAATTGGAAATGCATTTTTCCGCTGGCAAAGTGATGTTGATGATCCTCACACATTCATGGACCTCTTCGTGTCAAATGCTGATCCGGTTTTGCAGATGAGGGCCTGTGCTTACTACCCTAGATACGGATTGGGAGCGTTTGGAATTTTCCCAATGTTGCTGAAAAAAAGAATAACTTCTGAAGACTTTGGTGTTATGGGATTGAGATATGGCTCATCAAACTTGTCAGTTGGCGCCACTGTCATGCCCTTCTCCTTGAAAGATGATTGTCCAAGAAGGGCATGGTTGGTGAGCAAGATGGGAAGACTAACAGCTGGGGTGCAGTATGAGCCTGAATATGGGAGTAAAGATACTCAAAAGTACAAAAATTTAAAGAATTGGAGTGCTGCAATTGGTTATGGAGTGGGATCAAGCAGTCCTTTGAGTCCATCATTCAATTTTGGTCTCGAGCTGTCAAGAAGTTCTCAGTTCATTGCCTCATTCTATCAACATGTGGTGGTCCAAAGACGGGTGAAAAACCCCCTCGAAGAAAATGAAGTGGTTGGAATTACAAATTATATCGATTTTGGCTTTGAATTGCAGACAAGGGTTGATGATATCAAATCATCAAGCGATGTACCAGAATCAAATGATGTACCAGAATCAAGCAATGTACCTAAATCGAGCAATGGACCTAATGCCCCAGATTCTACCTTTCAAGTAGCTGCATCTTGGCAAGCCAATAAGAACTTCATGCTGAAGGGAAAGGTGGGCCCTCTCAGCTCATCGATTGCATTGGCATTTAAATCCTGGTGGAAACCTTCTTTCACATTCAGCGTTTCAGCTACGAGAAATCACGTTGTTGGGGAGACAGGTTATGGTTTTGGCCTTCGCGTGGAGAATCTTAGACAAGCCAGTTATCAAAGAGCCGATCCAAATTTCGTCATGTTGACACCGAATAAGGAGCACCTGGCAGAAGGCATTGTTTGGAAAGTTGGGAAAAGACCGATGCTACAGTCAGATATAAATGCTGGAAATTTCGACGGCATTCCGAAGGAGCTAAGACCCCTGGGGAAAATCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

445

Amino Acids

49.49

Weight (kDa)

7.06

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012020)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27930 AT3G27930 AT3G27930
fragaria_vesca FvH4_6g50760
malus_domestica MD09G1032600.v1.1 MD17G1034200.v1.1
prunus_persica Prupe.3G285400_v2.0.a1
pyrus_communis pycom17g03160
rosa_chinensis RchiOBHm_Chr2g0171701
rosa_laevigata RLG00000022030
rosa_multiflora Rmu_ssc0000252.1_g000012
rosa_roxburghii Rroxscaffold_2G00080510
rosa_rugosa Rorug02G0555400
rosa_samantha Rh2BG641000 Rh2BG643500 Rh2CG609200 Rh2DG651900 Rh2DG657200
rosa_wichuraiana Rw2G052180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 215
AccB1I GGYRCC 1 cut(s) 503
AccBSI CCGCTC 1 cut(s) 247
AccII CGCG 1 cut(s) 1143
AciI CCGC 8 cut(s) 29, 32, 50, 53, 78, 82, 245, 292
AclWI GGATC 4 cut(s) 308, 347, 685, 1175
AcsI RAATTY 4 cut(s) 414, 637, 1186, 1285
AcuI CTGAAG 3 cut(s) 215, 471, 1028
AcyI GRCGYC 1 cut(s) 504
AfaI GTAC 5 cut(s) 48, 632, 888, 906, 924
AfiI CCNNNNNNNGG 5 cut(s) 87, 395, 608, 778, 1144
AflIII ACRYGT 1 cut(s) 760
AgsI TTSAA 6 cut(s) 136, 161, 529, 706, 845, 968
AjiI CACGTC 1 cut(s) 217
AjnI CCWGG 4 cut(s) 86, 1058, 1218, 1317
AjuI GAANNNNNNNTTGG 2 cut(s) 983, 1015
AluBI AGCT 8 cut(s) 127, 167, 584, 721, 974, 1032, 1094, 1309
AluI AGCT 8 cut(s) 127, 167, 584, 721, 974, 1032, 1094, 1309
Alw21I GWGCWC 2 cut(s) 45, 1218
Alw26I GTCTC 2 cut(s) 719, 1112
AlwI GGATC 4 cut(s) 308, 347, 685, 1175
Ama87I CYCGRG 2 cut(s) 260, 716
AoxI GGCC 3 cut(s) 372, 1021, 1135
ApaI GGGCCC 1 cut(s) 1025
ApeKI GCWGC 2 cut(s) 656, 974
ApoI RAATTY 4 cut(s) 414, 637, 1186, 1285
ArsI GACNNNNNNTTYG 4 cut(s) 101, 133, 140, 172
Asp700I GAANNNNTTC 1 cut(s) 445
AspLEI GCGC 1 cut(s) 506
AspS9I GGNCC 7 cut(s) 242, 331, 372, 769, 941, 1021, 1022
AsuHPI GGTGA 2 cut(s) 571, 793
AvaI CYCGRG 2 cut(s) 260, 716
AvaII GGWCC 4 cut(s) 242, 331, 769, 941
BaeGI GKGCMC 1 cut(s) 1025
BanI GGYRCC 1 cut(s) 503
BanII GRGCYC 1 cut(s) 1025
BbsI GAAGAC 2 cut(s) 459, 580
Bbv12I GWGCWC 2 cut(s) 45, 1218
BbvI GCAGC 2 cut(s) 643, 961
BccI CCATC 4 cut(s) 31, 209, 562, 706
BceAI ACGGC 1 cut(s) 1309
BcgI CGANNNNNNTGC 2 cut(s) 209, 243
BciT130I CCWGG 4 cut(s) 88, 1060, 1220, 1319
BclI TGATCA 1 cut(s) 186
BcoDI GTCTC 2 cut(s) 719, 1112
BfaI CTAG 1 cut(s) 390
BfmI CTRYAG 1 cut(s) 1262
BfoI RGCGCY 1 cut(s) 507
BisI GCNGC 5 cut(s) 32, 53, 78, 657, 975
BlsI GCNGC 5 cut(s) 33, 54, 79, 658, 976
Bme1390I CCNGG 4 cut(s) 88, 1060, 1220, 1319
Bme18I GGWCC 4 cut(s) 242, 331, 769, 941
BmeT110I CYCGRG 2 cut(s) 260, 716
BmgBI CACGTC 1 cut(s) 217
BmgT120I GGNCC 7 cut(s) 242, 331, 372, 769, 941, 1021, 1022
BmiI GGNNCC 3 cut(s) 24, 505, 1023
BmrFI CCNGG 4 cut(s) 88, 1060, 1220, 1319
BmsI GCATC 2 cut(s) 986, 1248
BpiI GAAGAC 2 cut(s) 459, 580
Bsa29I ATCGAT 2 cut(s) 831, 1037
BsaHI GRCGYC 1 cut(s) 504
BsaI GGTCTC 1 cut(s) 719
BsaJI CCNNGG 3 cut(s) 261, 1317, 1318
BsaWI WCCGGW 1 cut(s) 355
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 5 cut(s) 87, 395, 608, 778, 1144
Bse1I ACTGG 1 cut(s) 1164
Bse3DI GCAATG 3 cut(s) 741, 925, 943
BseBI CCWGG 4 cut(s) 88, 1060, 1220, 1319
BseCI ATCGAT 2 cut(s) 831, 1037
BseDI CCNNGG 3 cut(s) 261, 1317, 1318
BseGI GGATG 1 cut(s) 96
BseLI CCNNNNNNNGG 5 cut(s) 87, 395, 608, 778, 1144
BseMI GCAATG 3 cut(s) 741, 925, 943
BseMII CTCAG 2 cut(s) 749, 1042
BseNI ACTGG 1 cut(s) 1164
BseRI GAGGAG 2 cut(s) 60, 278
BseSI GKGCMC 1 cut(s) 1025
BseXI GCAGC 2 cut(s) 643, 961
BseYI CCCAGC 1 cut(s) 584
BsgI GTGCAG 1 cut(s) 611
Bsh1236I CGCG 1 cut(s) 1143
BshFI GGCC 3 cut(s) 374, 1023, 1137
BshNI GGYRCC 1 cut(s) 503
BshVI ATCGAT 2 cut(s) 831, 1037
BsiHKAI GWGCWC 2 cut(s) 45, 1218
BsiHKCI CYCGRG 2 cut(s) 260, 716
BsiSI CCGG 1 cut(s) 356
BslI CCNNNNNNNGG 5 cut(s) 87, 395, 608, 778, 1144
BsmAI GTCTC 2 cut(s) 719, 1112
BsmI GAATGC 1 cut(s) 1296
BsnI GGCC 3 cut(s) 374, 1023, 1137
Bso31I GGTCTC 1 cut(s) 719
BsoBI CYCGRG 2 cut(s) 260, 716
Bsp120I GGGCCC 1 cut(s) 1021
Bsp1286I GDGCHC 3 cut(s) 45, 1025, 1218
Bsp143I GATC 6 cut(s) 186, 250, 313, 352, 677, 1180
BspACI CCGC 8 cut(s) 29, 32, 50, 53, 78, 82, 245, 292
BspANI GGCC 3 cut(s) 374, 1023, 1137
BspCNI CTCAG 2 cut(s) 748, 1041
BspDI ATCGAT 2 cut(s) 831, 1037
BspFNI CGCG 1 cut(s) 1143
BspLI GGNNCC 3 cut(s) 24, 505, 1023
BspPI GGATC 4 cut(s) 308, 347, 685, 1175
BspT107I GGYRCC 1 cut(s) 503
BspTNI GGTCTC 1 cut(s) 719
BsrBI CCGCTC 1 cut(s) 247
BsrDI GCAATG 3 cut(s) 741, 925, 943
BsrI ACTGG 1 cut(s) 1164
BssECI CCNNGG 3 cut(s) 261, 1317, 1318
BssMI GATC 6 cut(s) 186, 250, 313, 352, 677, 1180
BssNI GRCGYC 1 cut(s) 504
Bst2UI CCWGG 4 cut(s) 88, 1060, 1220, 1319
Bst4CI ACNGT 3 cut(s) 235, 511, 1266
Bst6I CTCTTC 1 cut(s) 341
BstACI GRCGYC 1 cut(s) 504
BstAPI GCANNNNNTGC 1 cut(s) 236
BstC8I GCNNGC 4 cut(s) 82, 129, 296, 987
BstDEI CTNAG 4 cut(s) 735, 1028, 1154, 1310
BstF5I GGATG 1 cut(s) 96
BstFNI CGCG 1 cut(s) 1143
BstH2I RGCGCY 1 cut(s) 507
BstHHI GCGC 1 cut(s) 506
BstKTI GATC 6 cut(s) 189, 253, 316, 355, 680, 1183
BstMAI GTCTC 2 cut(s) 719, 1112
BstMBI GATC 6 cut(s) 186, 250, 313, 352, 677, 1180
BstMWI GCNNNNNNNGC 6 cut(s) 31, 40, 49, 236, 598, 1029
BstNI CCWGG 4 cut(s) 88, 1060, 1220, 1319
BstNSI RCATGY 1 cut(s) 764
BstSCI CCNGG 4 cut(s) 86, 1058, 1218, 1317
BstSFI CTRYAG 1 cut(s) 1262
BstSLI GKGCMC 1 cut(s) 1025
BstUI CGCG 1 cut(s) 1143
BstV1I GCAGC 2 cut(s) 643, 961
BstV2I GAAGAC 2 cut(s) 459, 580
BstXI CCANNNNNNTGG 1 cut(s) 94
Bsu15I ATCGAT 2 cut(s) 831, 1037
BsuRI GGCC 3 cut(s) 374, 1023, 1137
BsuTUI ATCGAT 2 cut(s) 831, 1037
BtgZI GCGATG 1 cut(s) 897
BtrI CACGTC 1 cut(s) 217
BtsCI GGATG 1 cut(s) 96
BtsIMutI CAGTG 1 cut(s) 507
Cac8I GCNNGC 4 cut(s) 82, 129, 296, 987
CfoI GCGC 1 cut(s) 506
Cfr13I GGNCC 7 cut(s) 242, 331, 372, 769, 941, 1021, 1022
ClaI ATCGAT 2 cut(s) 831, 1037
Csp6I GTAC 5 cut(s) 47, 631, 887, 905, 923
CviAII CATG 7 cut(s) 190, 328, 514, 552, 761, 1003, 1195
CviQI GTAC 5 cut(s) 47, 631, 887, 905, 923
DdeI CTNAG 4 cut(s) 735, 1028, 1154, 1310
DinI GGCGCC 1 cut(s) 505
DpnI GATC 6 cut(s) 188, 252, 315, 354, 679, 1182
DpnII GATC 6 cut(s) 186, 250, 313, 352, 677, 1180
DraI TTTAAA 2 cut(s) 642, 1054
DrdI GACNNNNNNGTC 1 cut(s) 215
DseDI GACNNNNNNGTC 1 cut(s) 215
Eam1104I CTCTTC 1 cut(s) 341
EarI CTCTTC 1 cut(s) 341
Eco24I GRGCYC 1 cut(s) 1025
Eco31I GGTCTC 1 cut(s) 719
Eco32I GATATC 1 cut(s) 868
Eco47I GGWCC 4 cut(s) 242, 331, 769, 941
Eco57I CTGAAG 3 cut(s) 215, 471, 1028
Eco88I CYCGRG 2 cut(s) 260, 716
EcoO109I RGGNCCY 2 cut(s) 372, 1022
EcoRII CCWGG 4 cut(s) 86, 1058, 1218, 1317
EcoRV GATATC 1 cut(s) 868
EcoT22I ATGCAT 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 1025
EgeI GGCGCC 1 cut(s) 505
EheI GGCGCC 1 cut(s) 505
FaeI CATG 7 cut(s) 193, 331, 517, 555, 764, 1006, 1198
FalI AAGNNNNNCTT 4 cut(s) 430, 462, 673, 705
FatI CATG 7 cut(s) 189, 327, 513, 551, 760, 1002, 1194
FauI CCCGC 1 cut(s) 89
FbaI TGATCA 1 cut(s) 186
Fnu4HI GCNGC 5 cut(s) 32, 53, 78, 657, 975
FokI GGATG 1 cut(s) 103
FriOI GRGCYC 1 cut(s) 1025
Fsp4HI GCNGC 5 cut(s) 32, 53, 78, 657, 975
FspBI CTAG 1 cut(s) 390
GlaI GCGC 1 cut(s) 505
GluI GCNGC 5 cut(s) 32, 53, 78, 657, 975
GsaI CCCAGC 1 cut(s) 588
HaeII RGCGCY 1 cut(s) 507
HaeIII GGCC 3 cut(s) 374, 1023, 1137
HapII CCGG 1 cut(s) 356
HhaI GCGC 1 cut(s) 506
Hin1I GRCGYC 1 cut(s) 504
Hin1II CATG 7 cut(s) 193, 331, 517, 555, 764, 1006, 1198
Hin6I GCGC 1 cut(s) 504
HinP1I GCGC 1 cut(s) 504
HincII GTYRAC 1 cut(s) 1200
HindII GTYRAC 1 cut(s) 1200
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 6 cut(s) 98, 694, 893, 911, 956, 1150
HpaII CCGG 1 cut(s) 356
HphI GGTGA 2 cut(s) 571, 793
Hpy166II GTNNAC 1 cut(s) 1200
Hpy188I TCNGA 3 cut(s) 451, 1270, 1302
Hpy188III TCNNGA 2 cut(s) 716, 726
Hpy8I GTNNAC 1 cut(s) 1200
Hpy99I CGWCG 4 cut(s) 106, 209, 221, 1295
HpyAV CCTTC 7 cut(s) 529, 540, 1003, 1080, 1148, 1220, 1297
HpyCH4III ACNGT 3 cut(s) 235, 511, 1266
HpyCH4IV ACGT 2 cut(s) 216, 1107
HpyCH4V TGCA 9 cut(s) 230, 239, 284, 364, 592, 659, 850, 977, 1043
HpyF10VI GCNNNNNNNGC 6 cut(s) 31, 40, 49, 236, 598, 1029
HpyF3I CTNAG 4 cut(s) 735, 1028, 1154, 1310
HpySE526I ACGT 2 cut(s) 216, 1107
Hsp92I GRCGYC 1 cut(s) 504
Hsp92II CATG 7 cut(s) 193, 331, 517, 555, 764, 1006, 1198
HspAI GCGC 1 cut(s) 504
KasI GGCGCC 1 cut(s) 503
Ksp22I TGATCA 1 cut(s) 186
Kzo9I GATC 6 cut(s) 186, 250, 313, 352, 677, 1180
LmnI GCTCC 4 cut(s) 22, 404, 1213, 1306
Lsp1109I GCAGC 2 cut(s) 643, 961
LweI GCATC 2 cut(s) 986, 1248
MaeI CTAG 1 cut(s) 390
MaeII ACGT 2 cut(s) 216, 1107
MalI GATC 6 cut(s) 188, 252, 315, 354, 679, 1182
MbiI CCGCTC 1 cut(s) 247
MboI GATC 6 cut(s) 186, 250, 313, 352, 677, 1180
MboII GAAGA 4 cut(s) 328, 464, 585, 809
MfeI CAATTG 1 cut(s) 660
MhlI GDGCHC 3 cut(s) 45, 1025, 1218
Mly113I GGCGCC 1 cut(s) 504
MlyI GAGTC 2 cut(s) 107, 703
MmeI TCCRAC 2 cut(s) 75, 793
Mph1103I ATGCAT 1 cut(s) 286
MroXI GAANNNNTTC 1 cut(s) 445
MseI TTAA 2 cut(s) 641, 1053
MslI CAYNNNNRTG 3 cut(s) 89, 326, 512
MspA1I CMGCKG 2 cut(s) 294, 584
MspI CCGG 1 cut(s) 356
MspR9I CCNGG 4 cut(s) 88, 1060, 1220, 1319
MunI CAATTG 1 cut(s) 660
Mva1269I GAATGC 1 cut(s) 1296
MvaI CCWGG 4 cut(s) 88, 1060, 1220, 1319
MvnI CGCG 1 cut(s) 1143
MwoI GCNNNNNNNGC 6 cut(s) 31, 40, 49, 236, 598, 1029
NarI GGCGCC 1 cut(s) 504
NdeII GATC 6 cut(s) 186, 250, 313, 352, 677, 1180
NlaIII CATG 7 cut(s) 193, 331, 517, 555, 764, 1006, 1198
NlaIV GGNNCC 3 cut(s) 24, 505, 1023
NmeAIII GCCGAG 1 cut(s) 148
NsiI ATGCAT 1 cut(s) 286
NspI RCATGY 1 cut(s) 764
PaeR7I CTCGAG 1 cut(s) 716
PasI CCCWGGG 1 cut(s) 1318
PciI ACATGT 1 cut(s) 760
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PctI GAATGC 1 cut(s) 1296
PdmI GAANNNNTTC 1 cut(s) 445
PfeI GAWTC 4 cut(s) 893, 911, 956, 1150
PkrI GCNGC 5 cut(s) 33, 54, 79, 658, 976
PleI GAGTC 2 cut(s) 106, 702
PluTI GGCGCC 1 cut(s) 507
PpsI GAGTC 2 cut(s) 106, 702
PscI ACATGT 1 cut(s) 760
Psp6I CCWGG 4 cut(s) 86, 1058, 1218, 1317
PspFI CCCAGC 1 cut(s) 584
PspGI CCWGG 4 cut(s) 86, 1058, 1218, 1317
PspN4I GGNNCC 3 cut(s) 24, 505, 1023
PspOMI GGGCCC 1 cut(s) 1021
PspPI GGNCC 7 cut(s) 242, 331, 372, 769, 941, 1021, 1022
PvuII CAGCTG 1 cut(s) 584
RsaI GTAC 5 cut(s) 48, 632, 888, 906, 924
RsaNI GTAC 5 cut(s) 47, 631, 887, 905, 923
RseI CAYNNNNRTG 3 cut(s) 89, 326, 512
SaqAI TTAA 2 cut(s) 641, 1053
SatI GCNGC 5 cut(s) 32, 53, 78, 657, 975
Sau3AI GATC 6 cut(s) 186, 250, 313, 352, 677, 1180
Sau96I GGNCC 7 cut(s) 242, 331, 372, 769, 941, 1021, 1022
SchI GAGTC 2 cut(s) 107, 703
ScrFI CCNGG 4 cut(s) 88, 1060, 1220, 1319
SduI GDGCHC 3 cut(s) 45, 1025, 1218
SfaNI GCATC 2 cut(s) 986, 1248
SfcI CTRYAG 1 cut(s) 1262
SfoI GGCGCC 1 cut(s) 505
Sfr274I CTCGAG 1 cut(s) 716
SinI GGWCC 4 cut(s) 242, 331, 769, 941
SlaI CTCGAG 1 cut(s) 716
SmiI ATTTAAAT 1 cut(s) 1054
SmiMI CAYNNNNRTG 3 cut(s) 89, 326, 512
SmlI CTYRAG 1 cut(s) 716
SmoI CTYRAG 1 cut(s) 716
SsiI CCGC 8 cut(s) 29, 32, 50, 53, 78, 82, 245, 292
SspDI GGCGCC 1 cut(s) 503
SspMI CTAG 1 cut(s) 390
StyD4I CCNGG 4 cut(s) 86, 1058, 1218, 1317
SwaI ATTTAAAT 1 cut(s) 1054
TaaI ACNGT 3 cut(s) 235, 511, 1266
TaiI ACGT 2 cut(s) 219, 1110
TaqII GACCGA 1 cut(s) 1270
TatI WGTACW 1 cut(s) 630
TauI GCSGC 3 cut(s) 34, 55, 80
TfiI GAWTC 4 cut(s) 893, 911, 956, 1150
Tru1I TTAA 2 cut(s) 641, 1053
Tru9I TTAA 2 cut(s) 641, 1053
TscAI CASTG 1 cut(s) 514
TseI GCWGC 2 cut(s) 656, 974
TspDTI ATGAA 4 cut(s) 316, 730, 819, 991
TspGWI ACGGA 1 cut(s) 411
TspRI CASTG 1 cut(s) 514
VpaK11BI GGWCC 4 cut(s) 242, 331, 769, 941
XapI RAATTY 4 cut(s) 414, 637, 1186, 1285
XceI RCATGY 1 cut(s) 764
XcmI CCANNNNNNNNNTGG 1 cut(s) 549
XhoI CTCGAG 1 cut(s) 716
XmnI GAANNNNTTC 1 cut(s) 445
XspI CTAG 1 cut(s) 390
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.